MS002400 (gene) Bitter gourd (TR) v1

BLAST of MS002400 vs. NCBI nr
Match: XP_022135522.1 (solute carrier family 35 member F5-like [Momordica charantia])

HSP 1 Score: 669.1 bits (1725), Expect = 2.1e-188
Identity = 344/344 (100.00%), Postives = 344/344 (100.00%), Query Frame = 0

Query: 1   QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ 60
           QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ
Sbjct: 29  QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ 88

Query: 61  KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG 120
           KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG
Sbjct: 89  KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG 148

Query: 121 FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 180
           FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS
Sbjct: 149 FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 208

Query: 181 MAGVAMTTLGKTSAADEKNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNLDM 240
           MAGVAMTTLGKTSAADEKNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNLDM
Sbjct: 209 MAGVAMTTLGKTSAADEKNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNLDM 268

Query: 241 QKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFWAL 300
           QKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFWAL
Sbjct: 269 QKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFWAL 328

Query: 301 AVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 345
           AVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV
Sbjct: 329 AVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 372

BLAST of MS002400 vs. NCBI nr
Match: XP_022933868.1 (uncharacterized vacuolar membrane protein YML018C-like [Cucurbita moschata])

HSP 1 Score: 612.5 bits (1578), Expect = 2.3e-171
Identity = 315/346 (91.04%), Postives = 331/346 (95.66%), Query Frame = 0

Query: 1   QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ 60
           QSIFADYEQPFAVSYVTTSLLIVYLPIA LKDWL++F Y C+ KCG+N N V QSS+ELQ
Sbjct: 27  QSIFADYEQPFAVSYVTTSLLIVYLPIAFLKDWLMSFFYCCNSKCGDNLNVVYQSSVELQ 86

Query: 61  KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG 120
           K EVN ASEIEHQGQLSCKNCTIDMY+KDEG PLVA+HI KENTLK+DRKFTAKEVAAFG
Sbjct: 87  KVEVNFASEIEHQGQLSCKNCTIDMYSKDEGTPLVAVHIDKENTLKRDRKFTAKEVAAFG 146

Query: 121 FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 180
           FCIAP+WFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS
Sbjct: 147 FCIAPIWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 206

Query: 181 MAGVAMTTLGKTSAADE--KNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 240
           MAGVAMTT GKTSAADE  KNGNG +N+ALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL
Sbjct: 207 MAGVAMTTFGKTSAADELQKNGNGIENNALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 266

Query: 241 DMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFW 300
           DMQKLFGCIGLFTFVALWWLVWPLTAMG+EPRF+FPHSANVE+VILVNAFVGSFLSDYFW
Sbjct: 267 DMQKLFGCIGLFTFVALWWLVWPLTAMGVEPRFVFPHSANVEKVILVNAFVGSFLSDYFW 326

Query: 301 ALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 345
           ALAVVWTSPLVAALGVSLTIPVAMLEDM+IHGRQYSA+YIIGSAQV
Sbjct: 327 ALAVVWTSPLVAALGVSLTIPVAMLEDMIIHGRQYSAIYIIGSAQV 372

BLAST of MS002400 vs. NCBI nr
Match: XP_023007431.1 (uncharacterized vacuolar membrane protein YML018C-like [Cucurbita maxima])

HSP 1 Score: 610.9 bits (1574), Expect = 6.7e-171
Identity = 314/346 (90.75%), Postives = 331/346 (95.66%), Query Frame = 0

Query: 1   QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ 60
           QSIFADYEQPFAVSYVTTSLLIVYLPIA LKDWL++F Y C+ KCG+N N V QSS+ELQ
Sbjct: 27  QSIFADYEQPFAVSYVTTSLLIVYLPIAFLKDWLMSFFYCCNSKCGDNLNVVYQSSVELQ 86

Query: 61  KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG 120
           K EV+ ASEIEHQGQLSCKNCTIDMY+KDEG PLVA+HI KENTLK+DRKFTAKEVAAFG
Sbjct: 87  KVEVSFASEIEHQGQLSCKNCTIDMYSKDEGTPLVAVHIDKENTLKRDRKFTAKEVAAFG 146

Query: 121 FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 180
           FCIAP+WFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS
Sbjct: 147 FCIAPIWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 206

Query: 181 MAGVAMTTLGKTSAADE--KNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 240
           MAGVAMTT GKTSAADE  KNGNG +N+ALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL
Sbjct: 207 MAGVAMTTFGKTSAADELQKNGNGIENNALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 266

Query: 241 DMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFW 300
           DMQKLFGCIGLFTFVALWWLVWPLTAMG+EPRF+FPHSANVE+VILVNAFVGSFLSDYFW
Sbjct: 267 DMQKLFGCIGLFTFVALWWLVWPLTAMGVEPRFLFPHSANVEKVILVNAFVGSFLSDYFW 326

Query: 301 ALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 345
           ALAVVWTSPLVAALGVSLTIPVAMLEDM+IHGRQYSA+YIIGSAQV
Sbjct: 327 ALAVVWTSPLVAALGVSLTIPVAMLEDMIIHGRQYSAIYIIGSAQV 372

BLAST of MS002400 vs. NCBI nr
Match: XP_023531838.1 (uncharacterized transporter C405.03c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 609.4 bits (1570), Expect = 2.0e-170
Identity = 314/346 (90.75%), Postives = 330/346 (95.38%), Query Frame = 0

Query: 1   QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ 60
           QSIFADYEQPFAVSYVTTSLLIVYLPIA LKDWL++F Y C+ KCG+N N V QSS+ELQ
Sbjct: 27  QSIFADYEQPFAVSYVTTSLLIVYLPIAFLKDWLMSFFYCCNSKCGDNLNVVYQSSVELQ 86

Query: 61  KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG 120
           K EVN ASEIEHQ QLSCKNCTIDMY+KDEG PLVA+HI KENTLK+DRKFTAKEVAAFG
Sbjct: 87  KVEVNFASEIEHQEQLSCKNCTIDMYSKDEGTPLVAVHIDKENTLKRDRKFTAKEVAAFG 146

Query: 121 FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 180
           FCIAP+WFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS
Sbjct: 147 FCIAPIWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 206

Query: 181 MAGVAMTTLGKTSAADE--KNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 240
           MAGVAMTT GKTSAADE  KNGNG +N+ALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL
Sbjct: 207 MAGVAMTTFGKTSAADELQKNGNGIENNALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 266

Query: 241 DMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFW 300
           DMQKLFGCIGLFTFVALWWLVWPLTAMG+EPRF+FPHSANVE+VILVNAFVGSFLSDYFW
Sbjct: 267 DMQKLFGCIGLFTFVALWWLVWPLTAMGVEPRFVFPHSANVEKVILVNAFVGSFLSDYFW 326

Query: 301 ALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 345
           ALAVVWTSPLVAALGVSLTIPVAMLEDM+IHGRQYSA+YIIGSAQV
Sbjct: 327 ALAVVWTSPLVAALGVSLTIPVAMLEDMIIHGRQYSAIYIIGSAQV 372

BLAST of MS002400 vs. NCBI nr
Match: KAG6587871.1 (hypothetical protein SDJN03_16436, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 609.0 bits (1569), Expect = 2.5e-170
Identity = 313/346 (90.46%), Postives = 329/346 (95.09%), Query Frame = 0

Query: 1   QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ 60
           QSIFADYEQPFAVSYVTTSLLIVYLPIA LKDWL+ F Y C+ KCG+N N V QSS+ELQ
Sbjct: 27  QSIFADYEQPFAVSYVTTSLLIVYLPIAFLKDWLMRFFYCCNSKCGDNLNVVYQSSVELQ 86

Query: 61  KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG 120
           K EVN ASEIEHQ QLSCKNCTIDMY+KDEG PLVA+H+ KENTLK+DRKFTAKEVAAFG
Sbjct: 87  KVEVNFASEIEHQAQLSCKNCTIDMYSKDEGTPLVAVHVDKENTLKRDRKFTAKEVAAFG 146

Query: 121 FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 180
           FCIAP+WFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS
Sbjct: 147 FCIAPIWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 206

Query: 181 MAGVAMTTLGKTSAADE--KNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 240
           MAGVAMTT GKTSAADE  KNGNG +N+ALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL
Sbjct: 207 MAGVAMTTFGKTSAADELQKNGNGIENNALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 266

Query: 241 DMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFW 300
           DMQKLFGCIGLFTFVALWWLVWPLTAMG+EPRF+FPHSANVE+VILVNAFVGSFLSDYFW
Sbjct: 267 DMQKLFGCIGLFTFVALWWLVWPLTAMGVEPRFVFPHSANVEKVILVNAFVGSFLSDYFW 326

Query: 301 ALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 345
           ALAVVWTSPLVAALGVSLTIPVAMLEDM+IHGRQYSA+YIIGSAQV
Sbjct: 327 ALAVVWTSPLVAALGVSLTIPVAMLEDMIIHGRQYSAIYIIGSAQV 372

BLAST of MS002400 vs. ExPASy Swiss-Prot
Match: Q03730 (Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YML018C PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 3.4e-32
Identity = 111/353 (31.44%), Postives = 177/353 (50.14%), Query Frame = 0

Query: 3   IFAD--YEQPFAVSYVTTSLLIVYL-PIALLKDWLLTFLYRCDPKCGENPNAVDQSSIEL 62
           IF D  Y +PF ++Y  T+  I YL P A                      AV  +  + 
Sbjct: 37  IFEDDSYRKPFFITYTNTAAFIFYLFPTA---------------------KAVVVNYKDT 96

Query: 63  QKNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVA-IHIGKENTLKKDRKFTAKEVAA 122
            +  V+    +E +G  S  N ++DM +     PL+  +  G     KK          +
Sbjct: 97  GRANVHRELIMEEEGTGSDSNRSVDMTS-----PLLTNLEAGTHANQKKRLTLYETIKLS 156

Query: 123 FGFCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVV 182
             FCI  LWF    +TNA+LA TSVAS T+LS+TS  FTL IGA+   ++++  KV+   
Sbjct: 157 AEFCI--LWFTANLVTNASLAFTSVASQTILSTTSSFFTLFIGAICHVESLSKSKVLGSF 216

Query: 183 VSMAGVAMTTLG------KTSAADEKNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFA 242
           +S  G+ M T        +   AD    + +    L+GN+ ++  ++ YG+++ LLK+  
Sbjct: 217 ISFVGIIMVTKSDSHQRYQRHIADVSGDDNDAVQVLIGNLLALAGAVLYGVYSTLLKREV 276

Query: 243 GGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSF 302
           G    ++M+  FG +GLF  + LW  +  L   G EP F  P    V  +I VN  + +F
Sbjct: 277 GDETRVNMKIFFGFVGLFNLLFLWPSLIVLDFFGWEP-FSLPKDPKVVVIIFVNCLI-TF 336

Query: 303 LSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQVI 346
           +SD+ WA A++ TSPL   +G+S+TIP+AM  D++   +  SA+Y+ G+  ++
Sbjct: 337 VSDFCWAKAMLLTSPLTVTVGLSITIPLAMFGDVIFKHKTMSALYLFGATLIL 359

BLAST of MS002400 vs. ExPASy Swiss-Prot
Match: Q04083 (Thiamine-repressible mitochondrial transport protein THI74 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=THI74 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.5e-27
Identity = 78/221 (35.29%), Postives = 129/221 (58.37%), Query Frame = 0

Query: 121 FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 180
           FC+  LWF+     NAAL+ T+VAS+T+LSSTS  FTL +   LG +T +  K++ + VS
Sbjct: 124 FCV--LWFVANLAANAALSYTTVASSTILSSTSSFFTLFLATSLGIETFSTKKLLGLFVS 183

Query: 181 MAGVAMTTLGKTSAADEKNGNGNKNHALLGNVFSVLSSMTYGLFTVLLK-KFAGGGQNLD 240
           + G+ +  +  +   D  + +      L+GN  ++L S+ Y ++T LLK + +  G  LD
Sbjct: 184 LFGIILIVMQSSKQQDSVSASS----FLVGNTLALLGSLGYSVYTTLLKYEISSKGLRLD 243

Query: 241 MQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFWA 300
           +Q   G +G+FTF+  W ++  L    +E  F  P + ++  ++++N  +  F+SDYFW 
Sbjct: 244 IQMFLGYVGIFTFLLFWPILIILDITHME-TFELPSNFHISFLVMLNCII-IFVSDYFWC 303

Query: 301 LAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIG 341
            A++ TSPLV  + ++ TIP+AM  D V     ++  YIIG
Sbjct: 304 KALILTSPLVVTVALTFTIPLAMFADFVWREAFFTPWYIIG 336

BLAST of MS002400 vs. ExPASy Swiss-Prot
Match: O94654 (Uncharacterized transporter C405.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC405.03c PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.6e-26
Identity = 74/227 (32.60%), Postives = 134/227 (59.03%), Query Frame = 0

Query: 118 AFGFCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSV 177
           + GFCI  +WF   Y +N++L  T+VAS T++SS SG FTL +G ++  +   + K++++
Sbjct: 106 SLGFCI--IWFAANYFSNSSLGFTNVASFTIISSMSGFFTLGLGTIVNVERFTLSKLLAL 165

Query: 178 VVSMAGVAMTTLGKTSAADEKNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQN 237
           + S+ GV +        AD  +   ++    LGN +++L+++ YG ++V++K        
Sbjct: 166 MASVGGVIIVVTQDAKQADLNDSPPSR--PALGNAYALLAALLYGCYSVMVKFHITEESC 225

Query: 238 LDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYF 297
           +  +  FG +GLF  + LW  +  L   G+E RF  P +     V+++NA + +F+SDY 
Sbjct: 226 VSTRLFFGLVGLFDLILLWPFLIILHLYGVE-RFSLPSTTAGLIVLIINASI-TFVSDYL 285

Query: 298 WALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 345
           W +A++ TSPL+  +G+SL+IP+A+  D+++ G   +   I+GS  V
Sbjct: 286 WVIAMLMTSPLLVTVGMSLSIPLALFFDILLKGHYLNFSLILGSLLV 326

BLAST of MS002400 vs. ExPASy Swiss-Prot
Match: A6QL92 (Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.8e-26
Identity = 79/247 (31.98%), Postives = 134/247 (54.25%), Query Frame = 0

Query: 101 KENTLKKDRKFTAKEVAAFGFCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLI 160
           +E+ LK   K TA +VA   F    +WFL  +    AL+ T VA   +LSSTSGLFTL++
Sbjct: 226 QESLLKTVGKLTATQVAKISFFFCFVWFLANFSYQEALSDTQVAIVNILSSTSGLFTLIL 285

Query: 161 GALL---GEDTINIVKVVSVVVSMAGVAMTTLGKTSAADEKNGNGNKNHALLGNVFSVLS 220
            A+      D   + K+++V++S+ GV +  L  +  +  +N         +G+++S++ 
Sbjct: 286 AAMFPSNSGDRFTLSKLLAVILSIGGVVLVNLSGSEKSPGRN--------TIGSIWSLVG 345

Query: 221 SMTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSA 280
           +M Y ++ V++K+       LD+   FG +GLF  + LW   + L   G E  F FP+  
Sbjct: 346 AMLYAVYIVMIKRKVDREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFED-FEFPNKV 405

Query: 281 NVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVY 340
            V   I++N  +G+ LS++ W      TS L+  L +SLTIP++++ DM +   Q+S ++
Sbjct: 406 -VLMCIVINGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLF 462

Query: 341 IIGSAQV 345
             G+  V
Sbjct: 466 FAGAIPV 462

BLAST of MS002400 vs. ExPASy Swiss-Prot
Match: Q4R794 (Solute carrier family 35 member F5 OS=Macaca fascicularis OX=9541 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.8e-25
Identity = 81/247 (32.79%), Postives = 133/247 (53.85%), Query Frame = 0

Query: 101 KENTLKKDRKFTAKEVAAFGFCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLI 160
           +E+ LK   K TA +VA   F    +WFL       AL+ T VA   +LSSTSGLFTL++
Sbjct: 134 QESILKTVGKLTATQVAKISFFFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLIL 193

Query: 161 GALL---GEDTINIVKVVSVVVSMAGVAMTTLGKTSAADEKNGNGNKNHALLGNVFSVLS 220
            A+      D   + K+++V++S+ GV +  L    +  EK+   N     +G+++S+  
Sbjct: 194 AAVFPSNSGDRFTLSKLLAVILSIGGVVLVNL----SGSEKSAGRN----TIGSIWSLAG 253

Query: 221 SMTYGLFTVLLKKFAGGGQNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSA 280
           +M Y ++ V++K+       LD+   FG +GLF  + LW   + L   G E  F FP+  
Sbjct: 254 AMLYAVYIVMIKRKVDREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFED-FEFPNKV 313

Query: 281 NVEEVILVNAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVY 340
            V   I++N  +G+ LS++ W      TS L+  L +SLTIP++++ DM +   Q+S ++
Sbjct: 314 -VLMCIIINGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLF 370

Query: 341 IIGSAQV 345
             G+  V
Sbjct: 374 FAGAIPV 370

BLAST of MS002400 vs. ExPASy TrEMBL
Match: A0A6J1C2Y0 (solute carrier family 35 member F5-like OS=Momordica charantia OX=3673 GN=LOC111007457 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.0e-188
Identity = 344/344 (100.00%), Postives = 344/344 (100.00%), Query Frame = 0

Query: 1   QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ 60
           QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ
Sbjct: 29  QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ 88

Query: 61  KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG 120
           KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG
Sbjct: 89  KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG 148

Query: 121 FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 180
           FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS
Sbjct: 149 FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 208

Query: 181 MAGVAMTTLGKTSAADEKNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNLDM 240
           MAGVAMTTLGKTSAADEKNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNLDM
Sbjct: 209 MAGVAMTTLGKTSAADEKNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNLDM 268

Query: 241 QKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFWAL 300
           QKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFWAL
Sbjct: 269 QKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFWAL 328

Query: 301 AVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 345
           AVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV
Sbjct: 329 AVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 372

BLAST of MS002400 vs. ExPASy TrEMBL
Match: A0A6J1F619 (uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita moschata OX=3662 GN=LOC111441155 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.1e-171
Identity = 315/346 (91.04%), Postives = 331/346 (95.66%), Query Frame = 0

Query: 1   QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ 60
           QSIFADYEQPFAVSYVTTSLLIVYLPIA LKDWL++F Y C+ KCG+N N V QSS+ELQ
Sbjct: 27  QSIFADYEQPFAVSYVTTSLLIVYLPIAFLKDWLMSFFYCCNSKCGDNLNVVYQSSVELQ 86

Query: 61  KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG 120
           K EVN ASEIEHQGQLSCKNCTIDMY+KDEG PLVA+HI KENTLK+DRKFTAKEVAAFG
Sbjct: 87  KVEVNFASEIEHQGQLSCKNCTIDMYSKDEGTPLVAVHIDKENTLKRDRKFTAKEVAAFG 146

Query: 121 FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 180
           FCIAP+WFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS
Sbjct: 147 FCIAPIWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 206

Query: 181 MAGVAMTTLGKTSAADE--KNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 240
           MAGVAMTT GKTSAADE  KNGNG +N+ALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL
Sbjct: 207 MAGVAMTTFGKTSAADELQKNGNGIENNALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 266

Query: 241 DMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFW 300
           DMQKLFGCIGLFTFVALWWLVWPLTAMG+EPRF+FPHSANVE+VILVNAFVGSFLSDYFW
Sbjct: 267 DMQKLFGCIGLFTFVALWWLVWPLTAMGVEPRFVFPHSANVEKVILVNAFVGSFLSDYFW 326

Query: 301 ALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 345
           ALAVVWTSPLVAALGVSLTIPVAMLEDM+IHGRQYSA+YIIGSAQV
Sbjct: 327 ALAVVWTSPLVAALGVSLTIPVAMLEDMIIHGRQYSAIYIIGSAQV 372

BLAST of MS002400 vs. ExPASy TrEMBL
Match: A0A6J1L0I2 (uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita maxima OX=3661 GN=LOC111499930 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.2e-171
Identity = 314/346 (90.75%), Postives = 331/346 (95.66%), Query Frame = 0

Query: 1   QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ 60
           QSIFADYEQPFAVSYVTTSLLIVYLPIA LKDWL++F Y C+ KCG+N N V QSS+ELQ
Sbjct: 27  QSIFADYEQPFAVSYVTTSLLIVYLPIAFLKDWLMSFFYCCNSKCGDNLNVVYQSSVELQ 86

Query: 61  KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG 120
           K EV+ ASEIEHQGQLSCKNCTIDMY+KDEG PLVA+HI KENTLK+DRKFTAKEVAAFG
Sbjct: 87  KVEVSFASEIEHQGQLSCKNCTIDMYSKDEGTPLVAVHIDKENTLKRDRKFTAKEVAAFG 146

Query: 121 FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 180
           FCIAP+WFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS
Sbjct: 147 FCIAPIWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 206

Query: 181 MAGVAMTTLGKTSAADE--KNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 240
           MAGVAMTT GKTSAADE  KNGNG +N+ALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL
Sbjct: 207 MAGVAMTTFGKTSAADELQKNGNGIENNALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 266

Query: 241 DMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFW 300
           DMQKLFGCIGLFTFVALWWLVWPLTAMG+EPRF+FPHSANVE+VILVNAFVGSFLSDYFW
Sbjct: 267 DMQKLFGCIGLFTFVALWWLVWPLTAMGVEPRFLFPHSANVEKVILVNAFVGSFLSDYFW 326

Query: 301 ALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 345
           ALAVVWTSPLVAALGVSLTIPVAMLEDM+IHGRQYSA+YIIGSAQV
Sbjct: 327 ALAVVWTSPLVAALGVSLTIPVAMLEDMIIHGRQYSAIYIIGSAQV 372

BLAST of MS002400 vs. ExPASy TrEMBL
Match: A0A0A0LVW6 (EamA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G222870 PE=3 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 1.1e-166
Identity = 307/346 (88.73%), Postives = 329/346 (95.09%), Query Frame = 0

Query: 1   QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ 60
           QSIFADYEQPFA+SYVTTSL IVYLPIALLKDWLL+FL+R + K G+  + V QSS+ELQ
Sbjct: 45  QSIFADYEQPFAISYVTTSLWIVYLPIALLKDWLLSFLHRYNSKRGD-LSVVGQSSVELQ 104

Query: 61  KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG 120
           KNEVN+ASE+E QG+LSCKNCT+D+Y+KDEG PLVA+HIGKE TLKKDRKFTAKEVAAFG
Sbjct: 105 KNEVNIASELEPQGELSCKNCTVDVYSKDEGTPLVAVHIGKETTLKKDRKFTAKEVAAFG 164

Query: 121 FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 180
           FC+AP+WFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINI+KVVSVVVS
Sbjct: 165 FCVAPIWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIIKVVSVVVS 224

Query: 181 MAGVAMTTLGKTSAADE--KNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 240
           MAGVAMTT GKTSAADE  KN NGN+NHALLGNVFSVLSS+TYGLFTVLLKKFAGGGQNL
Sbjct: 225 MAGVAMTTFGKTSAADELQKNANGNENHALLGNVFSVLSSVTYGLFTVLLKKFAGGGQNL 284

Query: 241 DMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFW 300
           D+QKLFGCIGLFTFVALWWLVWPLTAMGIEPRF FPHSANVEEVILVNAFVGSFLSDYFW
Sbjct: 285 DLQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFTFPHSANVEEVILVNAFVGSFLSDYFW 344

Query: 301 ALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 345
           ALAVVWTSPLVAALGVSLTIP+AMLEDMVIHGRQYS +YIIGSAQV
Sbjct: 345 ALAVVWTSPLVAALGVSLTIPIAMLEDMVIHGRQYSVIYIIGSAQV 389

BLAST of MS002400 vs. ExPASy TrEMBL
Match: A0A5D3C3F3 (Putative transporter-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00200 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.1e-166
Identity = 307/346 (88.73%), Postives = 325/346 (93.93%), Query Frame = 0

Query: 1   QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ 60
           QSIF DYEQPFA+SYVTTSL IVYLPIALLKDWLL+FL+R + K G+    V QSS+ELQ
Sbjct: 27  QSIFEDYEQPFAISYVTTSLWIVYLPIALLKDWLLSFLHRYNSKRGD-LCVVGQSSVELQ 86

Query: 61  KNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAAFG 120
           KNEVN+ASE+EHQG+LSCKNCTID+Y+KDEG PLVA+HIGKENTLKKDRKFTAKEVAAFG
Sbjct: 87  KNEVNIASELEHQGELSCKNCTIDVYSKDEGAPLVAVHIGKENTLKKDRKFTAKEVAAFG 146

Query: 121 FCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVVVS 180
           FC+AP+WFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINI+KVVSVVVS
Sbjct: 147 FCVAPIWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIIKVVSVVVS 206

Query: 181 MAGVAMTTLGKTSAADE--KNGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAGGGQNL 240
           MAGV MTT GKTSAADE  KN NGN+NHALLGNVFSVLSS+TYGLFTVLLKKFAGGGQNL
Sbjct: 207 MAGVVMTTFGKTSAADELQKNANGNENHALLGNVFSVLSSVTYGLFTVLLKKFAGGGQNL 266

Query: 241 DMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSDYFW 300
           DMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRF FPHSANVEEVILVNAFVGSF SDYFW
Sbjct: 267 DMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFTFPHSANVEEVILVNAFVGSFFSDYFW 326

Query: 301 ALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 345
           ALAVVWTSPLVAALGVSLTIP AMLEDMVIHGRQYS +YIIGS QV
Sbjct: 327 ALAVVWTSPLVAALGVSLTIPFAMLEDMVIHGRQYSVIYIIGSVQV 371

BLAST of MS002400 vs. TAIR 10
Match: AT4G32140.1 (EamA-like transporter family )

HSP 1 Score: 386.0 bits (990), Expect = 3.3e-107
Identity = 203/349 (58.17%), Postives = 265/349 (75.93%), Query Frame = 0

Query: 1   QSIFADYEQPFAVSYVTTSLLIVYLPIALLKDWLLTFLYRCDPKCGENPNAVDQSSIELQ 60
           Q IF  Y+QPFAV+Y+  SL+IVYLP+A LKDWL  +L R   K  + P   D SS+EL 
Sbjct: 27  QDIFTAYKQPFAVTYLGASLMIVYLPVAFLKDWLCRYLDRRSSKSNKIPALTDDSSVELG 86

Query: 61  KNEVNLASEIEHQGQLSCKNCT--IDMYNKDEGKPLVAIHIGKENTLKKDRKFTAKEVAA 120
               +   E+  QG ++ K+    +  + +++ +PL+     +  TLK+ ++ T K++A 
Sbjct: 87  SPLRHKIIEMGLQGTITKKDSEEHLSSHEEEDERPLIGKVREEVQTLKQRKEITTKQIAM 146

Query: 121 FGFCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFTLLIGALLGEDTINIVKVVSVV 180
           +G  +AP+WF+TEYL+NAALARTSVASTT+LSSTSGLFTL IG  LG+DT+N+ KVV+V 
Sbjct: 147 YGLYLAPIWFVTEYLSNAALARTSVASTTVLSSTSGLFTLFIGVFLGQDTLNLSKVVAVF 206

Query: 181 VSMAGVAMTTLGKTSAADEK--NGNGNKNHALLGNVFSVLSSMTYGLFTVLLKKFAG-GG 240
           VSMAGV MTTLGKT A+DE   N + N   +L+G++F +LS+++YGLFTVLLKKFAG  G
Sbjct: 207 VSMAGVVMTTLGKTWASDESQLNSSLNGERSLMGDLFGLLSAVSYGLFTVLLKKFAGEEG 266

Query: 241 QNLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMFPHSANVEEVILVNAFVGSFLSD 300
           + +D+QKLFG IGLFT VALWWLVWPLTA+GIEP+F  PHS  V+EV+L N F+GS LSD
Sbjct: 267 EGVDVQKLFGYIGLFTLVALWWLVWPLTALGIEPKFTIPHSVKVDEVVLANGFIGSVLSD 326

Query: 301 YFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGRQYSAVYIIGSAQV 345
           YFWAL+VVWT+PLVA LG+SLTIP+AM+ DM+IHGR YSA+YI+GS QV
Sbjct: 327 YFWALSVVWTTPLVATLGMSLTIPLAMVADMMIHGRHYSAIYILGSTQV 375

BLAST of MS002400 vs. TAIR 10
Match: AT3G07080.1 (EamA-like transporter family )

HSP 1 Score: 93.6 bits (231), Expect = 3.4e-19
Identity = 92/374 (24.60%), Postives = 169/374 (45.19%), Query Frame = 0

Query: 1   QSIFADYEQPFAVSYVTTSLLIVYLPI----ALLKDWLLTFLYRCDPK------------ 60
           QS+      PF ++++  SL +VYLP+      L+D   + L+    +            
Sbjct: 30  QSVVEAGVSPFLITFICNSLFVVYLPLFEIGRYLEDAYGSLLFWRSKRSHLMELVESEKA 89

Query: 61  -------CGENPNAVDQSSIELQKNEVNLASEIEHQGQLSCKNCTIDMYNKDEGKPLVAI 120
                   G   +A + S + +++ E++        G  + +       N  +G   V+ 
Sbjct: 90  VLLGKDVSGVKSDATESSGLVVREQEISEGGNGIESGLENIELEIDASVNVSDGASGVS- 149

Query: 121 HIGKENTLKKDRKFTAKEVAAFGFCIAPLWFLTEYLTNAALARTSVASTTLLSSTSGLFT 180
                  L +  ++T   VA     I P WFL +   N +L  T+V S T+LSS S LFT
Sbjct: 150 ----NKGLDEKGRWTRMRVAKVSLVICPFWFLAQLTFNVSLKYTTVTSNTILSSASSLFT 209

Query: 181 LLIGALLGEDTINIVKVVSVVVSMAGVAMTTLGKTSAADEKNGNGNKNHALLGNVFSVLS 240
            L+  +   +    +K+ SV++ M+G  + ++G +    E   N    + LLG++ S++S
Sbjct: 210 FLVSLIFLGEKFTWLKLFSVLLCMSGTIIVSMGDS----ESKSNAVAKNPLLGDILSLVS 269

Query: 241 SMTYGLFTVLLKKFAGGGQ----NLDMQKLFGCIGLFTFVALWWLVWPLTAMGIEPRFMF 300
           +  Y ++  L++K           + M +  G +GLF F   +  + P   +    R  F
Sbjct: 270 AALYAVYITLIRKKLPDDDERNGRVSMAQFLGFLGLFNF---FIFLPPALILNFTKRERF 329

Query: 301 PHSANVEEVILV--NAFVGSFLSDYFWALAVVWTSPLVAALGVSLTIPVAMLEDMVIHGR 346
            ++  +++  LV     + + LSDY WA AV+ T+  VA  G+++ +P+A + D  + G 
Sbjct: 330 -NALTLKQFGLVVGKGLLDNVLSDYLWAKAVLLTTTTVATAGLTIQVPLAAIVDS-LSGN 389