MS002396 (gene) Bitter gourd (TR) v1

BLAST of MS002396 vs. NCBI nr
Match: XP_022134811.1 (uncharacterized protein LOC111006992 [Momordica charantia])

HSP 1 Score: 1016.1 bits (2626), Expect = 1.1e-292
Identity = 532/707 (75.25%), Postives = 537/707 (75.95%), Query Frame = 0

Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK
Sbjct: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60

Query: 61  LRHGLHSWGCFQVIS----------------VEFAL------------------------ 120
           LRHGLHSWGCFQ I+                + FAL                        
Sbjct: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120

Query: 121 ----------------------------LNP---RSGVRGYTANVKLLSEKILKAMARSL 180
                                        NP   R  +  YTANVKLLSEKILKAMARSL
Sbjct: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180

Query: 181 DLDENSFLNQYGERVQLDARFNFYPRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240
           DLDENSFLNQYG+RVQLDARFNFY RCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL
Sbjct: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240

Query: 241 KGNEWWNAPIIPDALLVNVGDQGE------------------------------------ 300
           KGNEWWNAPIIPDALLVNVGDQGE                                    
Sbjct: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300

Query: 301 -------------------------------------------------PSNMAELPDYF 360
                                                            PSNMAELPDYF
Sbjct: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKIISVHLRKPSNMAELPDYF 360

Query: 361 HTKTLQQKLLINGGDTPESYIYKDGYGGGDSNNNPLPLAEIPVVDLAQLSSSPPSTAALE 420
           HTKT QQKLLINGGDTPESYIYKDGYGGGDSNNNPLPLAEIPVVDLAQLSSSPPSTAALE
Sbjct: 361 HTKTFQQKLLINGGDTPESYIYKDGYGGGDSNNNPLPLAEIPVVDLAQLSSSPPSTAALE 420

Query: 421 DLRLALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSLPMEEKNKCCRELYGLEGYGTD 480
           DLRLALTSWGCFQ   INHSI SSFLNKI QISNQFFSLPMEEKNKCCRELYGLEGYGTD
Sbjct: 421 DLRLALTSWGCFQ--AINHSISSSFLNKIHQISNQFFSLPMEEKNKCCRELYGLEGYGTD 480

Query: 481 MVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMA 540
           MVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMA
Sbjct: 481 MVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMA 540

Query: 541 RSINVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGL 552
           RSINVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGL
Sbjct: 541 RSINVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGL 600

BLAST of MS002396 vs. NCBI nr
Match: KAG6589813.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 587.0 bits (1512), Expect = 1.7e-163
Identity = 322/688 (46.80%), Postives = 407/688 (59.16%), Query Frame = 0

Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MAGTNP+G+VQDVASKGEVPERYIHKE DRGA DAPLM APVID+ LLSS S +GPELEK
Sbjct: 20  MAGTNPSGTVQDVASKGEVPERYIHKESDRGARDAPLMAAPVIDMALLSSSSESGPELEK 79

Query: 61  LRHGLHSWGCFQVI----SVEFA------------------------------------- 120
           LRHGL SWGCFQV+    S EF                                      
Sbjct: 80  LRHGLQSWGCFQVVNHGMSAEFLDEIRRLAKQFFDLPMEEKSKYSREEDEIEGYGNDMIL 139

Query: 121 ---------------------------LLNP---RSGVRGYTANVKLLSEKILKAMARSL 180
                                        NP   R+ +  YTANVKLLSEKILKAMA SL
Sbjct: 140 SNQQILDWTDRLYLTVYPKESHRFKYWPTNPERFRAVLHEYTANVKLLSEKILKAMAISL 199

Query: 181 DLDENSFLNQYGERVQLDARFNFYPRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240
           DL+E+SF+ QYGE V+LDARFNFYPRCRNP+LVLGVKPHADGSAITILLQD+EVEGLQFL
Sbjct: 200 DLNEDSFIKQYGEEVKLDARFNFYPRCRNPDLVLGVKPHADGSAITILLQDEEVEGLQFL 259

Query: 241 KGNEWWNAPIIPDALLVNVGDQGEPSN-----------------------MAELPDYF-- 300
            GNEW+NAPI+P ALL+NVGDQ E ++                       +  LPD+   
Sbjct: 260 NGNEWFNAPIVPGALLINVGDQAEITSNGIFKSPVHRVLTNSERERISLAVFYLPDWEKE 319

Query: 301 -------------HTKTLQQKLLI----------------------------NGGDTPES 360
                          + + + L+I                            +  + PE 
Sbjct: 320 IEPLEKLIDETRPRREAITELLIIAAPSLPCLVAAPSSMAGSNPSSSVQEMGSKAEVPEG 379

Query: 361 YIYKDGYGGGDSNNNPLPLAEIPVVDLAQLSSSPPSTAALEDLRLALTSWGCFQVSTINH 420
           YI+K      D      PL +  V+D+  LS+SP S   LE LR  L +WGC  +  INH
Sbjct: 380 YIHKQ----RDRGAPDAPLMQASVIDIGLLSASPNSGPELEKLRHGLQTWGC--ILGINH 439

Query: 421 SIPSSFLNKIRQISNQFFSLPMEEKNKCCRELYGLEGYGTDMVFSEQQILDWTDRLYLKV 480
            +   FL  +R +  QFF+ PMEEK K   E   +EGYG D V S+ QILDW  RL+L +
Sbjct: 440 GMSPEFLEDVRHVIKQFFACPMEEKLKYSMEEAEIEGYGNDKVLSDTQILDWNHRLFLTL 499

Query: 481 NPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMARSINVEANSFTEQVGKRPE 540
            PE+ R++K+WP NP  FRE + E++  ++ + E +L AM RS+++E NSF EQ G+R E
Sbjct: 500 LPEESRRVKHWPSNPHRFREVIDEYSANMRVVCEKILKAMGRSLDLEENSFVEQFGERFE 559

Query: 541 LFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGLQWRKDDQWFRVPVPAMADS 552
           L  RFN YPPC  PDLVLG K H+D SAIT++L D +VEGLQ+ K D+WF  P+  + D+
Sbjct: 560 LAARFNLYPPCPNPDLVLGAKPHADVSAITVLLQDEQVEGLQFLKGDEWFNAPI--LPDA 619

BLAST of MS002396 vs. NCBI nr
Match: KAG6587867.1 (Protein SRG1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021754.1 Protein SRG1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 553.1 bits (1424), Expect = 2.7e-153
Identity = 267/346 (77.17%), Postives = 307/346 (88.73%), Query Frame = 0

Query: 207 KTLQQKLLINGGDTPESYIYKDGYGGGDSNNNPLPLAEIPVVDLAQLSSSPPSTAALEDL 266
           +TLQQ+LLINGGDTPESYIYK GY GGDSNN+PLP+AEIPVVDL+QLSSS    AALE+ 
Sbjct: 9   QTLQQQLLINGGDTPESYIYKGGYSGGDSNNDPLPMAEIPVVDLSQLSSSQGGEAALEEF 68

Query: 267 RLALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSLPMEEKNKCCRELYGLEGYGTDMV 326
           RLAL+SWGCFQ +  NH I SSFL K+RQIS QFF+LPMEEKN+  RE+ G EGYG+D++
Sbjct: 69  RLALSSWGCFQAT--NHGISSSFLEKMRQISEQFFALPMEEKNRYYREIDGHEGYGSDLI 128

Query: 327 FSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMARS 386
            SE QILDWTDRLYL VNPEDER+LK+WP+NP SFREDLHEFTIK+K+IIE VL+AMA S
Sbjct: 129 LSEPQILDWTDRLYLVVNPEDERKLKFWPENPPSFREDLHEFTIKVKEIIENVLIAMAAS 188

Query: 387 INVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGLQW 446
           + VE  SF++QVGKRP L TRFNFYPPCS P LVLGLKEHSDGSA TIVLLD+EVEGL+ 
Sbjct: 189 LKVEPKSFSDQVGKRPVLVTRFNFYPPCSTPHLVLGLKEHSDGSAFTIVLLDKEVEGLEM 248

Query: 447 RKDDQWFRVPVPAMADSLLINIGEQAEIMSNGVFKSAVHRAVTNSEKQRISVACFCCPEK 506
           +KD+QW+R+PVPA+ADSLLIN+GEQ EIMSNG+FKS VHRAVTNSE+QRISVACFCCPEK
Sbjct: 249 KKDNQWYRLPVPAIADSLLINVGEQGEIMSNGIFKSPVHRAVTNSERQRISVACFCCPEK 308

Query: 507 DREIEPIEGLIDERRPRLYRSVKNYV-GSYFQDYQKGQRPVDKLKI 552
           D+EI+PIEGLIDERRPRL+R+VKNYV  +YFQ YQKGQR VD+LKI
Sbjct: 309 DKEIKPIEGLIDERRPRLFRTVKNYVEETYFQYYQKGQRAVDELKI 352

BLAST of MS002396 vs. NCBI nr
Match: XP_011655280.1 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X2 [Cucumis sativus] >KAE8653014.1 hypothetical protein Csa_004557 [Cucumis sativus])

HSP 1 Score: 537.0 bits (1382), Expect = 2.0e-148
Identity = 259/356 (72.75%), Postives = 304/356 (85.39%), Query Frame = 0

Query: 197 MAELPDYFHTKTLQQKLLINGGDTPESYIYKDGY-GGGDSNNNPLPLAEIPVVDLAQLSS 256
           MA+      T+TLQQ+LLINGG TPESYIYK GY GGG +NN PLPLAEIPVVDL+QLSS
Sbjct: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSS 60

Query: 257 SPPSTAALEDLRLALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSLPMEEKNKCCREL 316
                  L DLRLAL++WGCFQ +  NHSI SSFL K+R+IS QFFSLP+EEK +  RE+
Sbjct: 61  PSAGEGPLNDLRLALSTWGCFQAT--NHSISSSFLEKLRKISEQFFSLPIEEKMRYGREV 120

Query: 317 YGLEGYGTDMVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQI 376
            G+EGYG D+ FS QQ LDW+DRLY   +PEDER+L  WP NP SFREDLHE+T+K+ +I
Sbjct: 121 DGMEGYGNDLTFSNQQTLDWSDRLYFVTSPEDERRLDLWPLNPPSFREDLHEYTVKIMEI 180

Query: 377 IETVLMAMARSINVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIV 436
           IETVL+AMARS+NVE NSFT+QVG+RP LFTRFNFYPPCS P LVLGLKEHSDGSAITI+
Sbjct: 181 IETVLIAMARSLNVEPNSFTDQVGERPTLFTRFNFYPPCSTPHLVLGLKEHSDGSAITIL 240

Query: 437 LLDREVEGLQWRKDDQWFRVPVPAMADSLLINIGEQAEIMSNGVFKSAVHRAVTNSEKQR 496
           LLD++VEGLQ RKDDQW+RVPVPA+ADSLL+ IGEQAE+MSNG+FKS+VHRAVTNSE+QR
Sbjct: 241 LLDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSVHRAVTNSERQR 300

Query: 497 ISVACFCCPEKDREIEPIEGLIDERRPRLYRSVKNYVGSYFQDYQKGQRPVDKLKI 552
           ISV CFCCPEKD EI+P+EGLIDE+RPRL+RSVKNY+ +YFQ+YQ+GQR VD L+I
Sbjct: 301 ISVVCFCCPEKDIEIKPVEGLIDEKRPRLFRSVKNYLETYFQNYQEGQRTVDGLRI 354

BLAST of MS002396 vs. NCBI nr
Match: XP_011655698.2 (probable 2-oxoglutarate-dependent dioxygenase ANS [Cucumis sativus] >KAE8653028.1 hypothetical protein Csa_020037 [Cucumis sativus])

HSP 1 Score: 536.2 bits (1380), Expect = 3.4e-148
Identity = 259/356 (72.75%), Postives = 305/356 (85.67%), Query Frame = 0

Query: 197 MAELPDYFHTKTLQQKLLINGGDTPESYIYKDGY-GGGDSNNNPLPLAEIPVVDLAQLSS 256
           MA+      T+TLQQ+LLINGG TPESYIYK GY GGG +NN PLPLAEIPVVDL+QLSS
Sbjct: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSS 60

Query: 257 SPPSTAALEDLRLALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSLPMEEKNKCCREL 316
                  L DLRLAL++WGCFQ   INHSI SSFL K+R+IS QFFSLP+EEK +  RE+
Sbjct: 61  PSAGEGPLNDLRLALSTWGCFQ--AINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREV 120

Query: 317 YGLEGYGTDMVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQI 376
            G+EGYG D++ SEQQILDW+DRLY   NPEDER+L+ WP NP SFREDL E+T+K+ +I
Sbjct: 121 DGMEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFREDLDEYTVKIMEI 180

Query: 377 IETVLMAMARSINVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIV 436
           IETVL+AMA S++VE NSFT+QVGKRP L TRFNFYPPCS P LVLGLKEHSDGSAITI+
Sbjct: 181 IETVLIAMASSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAITIL 240

Query: 437 LLDREVEGLQWRKDDQWFRVPVPAMADSLLINIGEQAEIMSNGVFKSAVHRAVTNSEKQR 496
           LLD++VEGLQ RKDDQW+RVPVPA+ADSLLI IGEQAE+MSNG+FKS++HRAVTNSE+QR
Sbjct: 241 LLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSERQR 300

Query: 497 ISVACFCCPEKDREIEPIEGLIDERRPRLYRSVKNYVGSYFQDYQKGQRPVDKLKI 552
           IS+ CFCCPEKD EI+PIEGLIDE+RPRL++SVKNY+ +YFQ+YQKG+RPVD L+I
Sbjct: 301 ISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGRRPVDGLRI 354

BLAST of MS002396 vs. ExPASy Swiss-Prot
Match: Q94LP4 (2-oxoglutarate-dependent dioxygenase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD11 PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 7.2e-53
Identity = 118/333 (35.44%), Postives = 180/333 (54.05%), Query Frame = 0

Query: 221 PESYIYKDGYGGGDSNNNPLPLAEIPVVDLAQLSSSPPSTAALEDLRLALTSWGCFQVST 280
           PE YI  +       NN    +A IP++DL +L     S      LR A   WG F +  
Sbjct: 30  PERYIRPEASSEEVINNYQGDMA-IPIIDLKKLLCPQSSEEECVKLRSACQYWGFFLL-- 89

Query: 281 INHSIPSSFLNKIRQISNQFFSLPMEEKNKCCRELYGLEGYGTDMVFSEQQILDWTDRLY 340
           INH +P   +  +++    FFS P++ K +  +    LEGYG   VFSE Q LDW D LY
Sbjct: 90  INHGVPDEVIANLKRDIVDFFSQPLDTKKEYTQLPNSLEGYGQSFVFSEDQKLDWADMLY 149

Query: 341 LKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMARSINVEANSFTEQVGK 400
           L V+P D R L++WP +P SFR+ +  ++ + K +   +   MA+++  +  S  +   +
Sbjct: 150 LHVHPSDSRDLRFWPTSPASFRQSIDAYSSETKSLALCLFEFMAKAVGAKPESLLDLFEE 209

Query: 401 RPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGLQWRKDDQWFRVPVPAM 460
           +P    R  +YPPC + D V+GL  HSD   +T++L    V+GLQ +KD +WF +  P  
Sbjct: 210 QPR-GLRMAYYPPCRQADKVMGLSPHSDAGGLTLLLEINNVQGLQIKKDGKWFSIDAPNG 269

Query: 461 ADSLLINIGEQAEIMSNGVFKSAVHRAVTNSEKQRISVACFCCPEKDREIEPIEGLIDER 520
           A  L+ NIG+  EI+SNG F+S  HRAV N  K+RIS A F  P ++  I P+   + + 
Sbjct: 270 A--LIANIGDTLEILSNGKFRSVEHRAVINPNKERISAALFHYPSENMVISPLPEFVKDG 329

Query: 521 RPRLYRSVK--NYVGSYFQDYQKGQRPVDKLKI 552
           + + YRS+   +++   F     G+  V+ LK+
Sbjct: 330 KVK-YRSISYLDFMKQIFTQQLDGKNRVEVLKL 355

BLAST of MS002396 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 9.4e-53
Identity = 108/311 (34.73%), Postives = 182/311 (58.52%), Query Frame = 0

Query: 244 EIPVVDLAQLSSSPPSTAALEDLRLALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSL 303
           EIP++D+ +L SS    + +E L  A   WG FQ+  +NH I SSFL+K++     FF+L
Sbjct: 52  EIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQL--VNHGIDSSFLDKVKSEIQDFFNL 111

Query: 304 PMEEKNKCCRELYGLEGYGTDMVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFRE 363
           PMEEK K  +    +EG+G   V SE Q LDW D  +  V P + R+   +P+ P  FR+
Sbjct: 112 PMEEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRD 171

Query: 364 DLHEFTIKLKQIIETVLMAMARSINVEANSFTEQVGKRPELFT-RFNFYPPCSRPDLVLG 423
            L  ++ +++ + + ++  MAR++ ++     +       + + R N+YPPC +PD V+G
Sbjct: 172 TLEMYSSEVQSVAKILIAKMARALEIKPEELEKLFDDVDSVQSMRMNYYPPCPQPDQVIG 231

Query: 424 LKEHSDGSAITIVLLDREVEGLQWRKDDQWFRVPVPAMADSLLINIGEQAEIMSNGVFKS 483
           L  HSD   +T+++   +VEGLQ +KD +W  VPV  + ++ ++NIG+  EI++NG ++S
Sbjct: 232 LTPHSDSVGLTVLMQVNDVEGLQIKKDGKW--VPVKPLPNAFIVNIGDVLEIITNGTYRS 291

Query: 484 AVHRAVTNSEKQRISVACFCCPEKDREIEPIEGLIDERRPRLYR--SVKNYVGSYFQDYQ 543
             HR V NSEK+R+S+A F      +E+ P + L++ ++   ++  ++K Y    F    
Sbjct: 292 IEHRGVVNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTL 351

Query: 544 KGQRPVDKLKI 552
            G+  +D L+I
Sbjct: 352 DGKAYLDALRI 358

BLAST of MS002396 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.8e-49
Identity = 106/310 (34.19%), Postives = 168/310 (54.19%), Query Frame = 0

Query: 244 EIPVVDLAQLSSSPPSTAALEDLRLALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSL 303
           EIPV+D+  +   P     L  +R A   WG FQ+  +NH +  S + ++R    +FF L
Sbjct: 47  EIPVLDMNDVWGKP---EGLRLVRSACEEWGFFQM--VNHGVTHSLMERVRGAWREFFEL 106

Query: 304 PMEEKNKCCRELYGLEGYGTDMVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFRE 363
           P+EEK K        EGYG+ +   +   LDW+D  +L   P   R    WP  P   RE
Sbjct: 107 PLEEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRE 166

Query: 364 DLHEFTIKLKQIIETVLMAMARSINVEANSFTEQVGKRPEL--FTRFNFYPPCSRPDLVL 423
            + ++  +++++ E +   ++ S+ ++ N   + +G   ++    R NFYP C +P L L
Sbjct: 167 LIEKYGEEVRKLCERLTETLSESLGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTL 226

Query: 424 GLKEHSDGSAITIVLLDREVEGLQWRKDDQWFRVPVPAMADSLLINIGEQAEIMSNGVFK 483
           GL  HSD   ITI+L D +V GLQ R+ D W  V + ++ ++L++NIG+Q +I+SNG++K
Sbjct: 227 GLSSHSDPGGITILLPDEKVAGLQVRRGDGW--VTIKSVPNALIVNIGDQLQILSNGIYK 286

Query: 484 SAVHRAVTNSEKQRISVACFCCPEKDREIEPIEGLIDERRPRLYRSVK--NYVGSYFQDY 543
           S  H+ + NS  +R+S+A F  P  D  + PIE L+   RP LY+ ++   Y     Q  
Sbjct: 287 SVEHQVIVNSGMERVSLAFFYNPRSDIPVGPIEELVTANRPALYKPIRFDEYRSLIRQKG 346

Query: 544 QKGQRPVDKL 550
             G+  VD L
Sbjct: 347 PCGKNQVDSL 349

BLAST of MS002396 vs. ExPASy Swiss-Prot
Match: A0A4D6Q440 (Flavonol synthase 1 OS=Crocosmia x crocosmiiflora OX=1053288 GN=FLS1 PE=1 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.7e-49
Identity = 105/301 (34.88%), Postives = 164/301 (54.49%), Query Frame = 0

Query: 241 PLAEIPVVDLAQLSSSPPSTAALEDLRLALTSWGCFQVSTINHSIPSSFLNKIRQISNQF 300
           P+ EIPV+DL        + A       A   WG FQ+  INH IP   + +++++  +F
Sbjct: 39  PIPEIPVIDLGDKDGIVGAVAE------AAREWGLFQI--INHGIPVEVIKELQRVGKEF 98

Query: 301 FSLPMEEKNKCCRELYGLEGYGTDMVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQS 360
           F LP EEK     ++  +EGYGT +    +    W D L+  + PE      +WPQNP  
Sbjct: 99  FELPQEEKEVYAMDMVTMEGYGTKLQKETEGKKAWVDHLFHNIWPESRINYNFWPQNPPD 158

Query: 361 FREDLHEFTIKLKQIIETVLMAMARSINVEANSFTEQV-GKRPELFTRFNFYPPCSRPDL 420
           +R+   E+   L +++  +L +++  + +E + F E + G   EL  + N+YP C RPDL
Sbjct: 159 YRKANKEYAKHLLEVMGEILTSLSLGLGLEGHVFKEALGGDAIELLLKINYYPTCPRPDL 218

Query: 421 VLGLKEHSDGSAITIVLLDREVEGLQWRKDDQWFRVPVPAMADSLLINIGEQAEIMSNGV 480
            LG+  H+D SAIT+ L+  EV GLQ  KDD WF      + ++++++IG+Q EI+SNG 
Sbjct: 219 ALGVPAHTDMSAITL-LVPNEVPGLQVFKDDHWF--DAKYIPNAIIVHIGDQIEILSNGK 278

Query: 481 FKSAVHRAVTNSEKQRISVACFCCPEKDREIEPIEGLIDERRPRLYRSVKNYVGSYFQDY 540
           +KS +HR   N EK R+S   FC P  +  +  +  L+ E  P  Y++ K      ++DY
Sbjct: 279 YKSVLHRTTVNKEKVRMSWPVFCSPPGEWMVGTLPQLVSEDTPAKYKTKK------YKDY 322

BLAST of MS002396 vs. ExPASy Swiss-Prot
Match: D4N502 (Codeine O-demethylase OS=Papaver somniferum OX=3469 GN=CODM PE=1 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.4e-48
Identity = 107/285 (37.54%), Postives = 162/285 (56.84%), Query Frame = 0

Query: 245 IPVVDLAQLSSSPPSTAALE--DLRLALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFS 304
           +PV+DL  L S  P    LE   L  A   WG FQ+  +NH + +  ++ I+     FF+
Sbjct: 54  VPVIDLQNLLSPEPVVGKLELDKLHSACKEWGFFQL--VNHGVDALLMDNIKSEIKGFFN 113

Query: 305 LPMEEKNKCCRELYGLEGYGTDMVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFR 364
           LPM EK K  ++    EG+G   + SE Q LDWT+   +   P   R+   +P+ P  FR
Sbjct: 114 LPMNEKTKYGQQDGDFEGFGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFR 173

Query: 365 EDLHEFTIKLKQIIETVLMAMARSIN-VEANSFTEQVGKRPELFTRFNFYPPCSRPDLVL 424
           E L  +  K+K++   V   + +S+  VE    T+      +   R N+YPPC RP+LVL
Sbjct: 174 ETLESYLSKMKKLSTVVFEMLEKSLQLVEIKGMTDLFEDGLQTM-RMNYYPPCPRPELVL 233

Query: 425 GLKEHSDGSAITIVLLDREVEGLQWRKDDQWFRVPVPAMADSLLINIGEQAEIMSNGVFK 484
           GL  HSD S +TI+L   EVEGLQ RK+++W  + +  + D+ ++N+G+  EIM+NG+++
Sbjct: 234 GLTSHSDFSGLTILLQLNEVEGLQIRKEERW--ISIKPLPDAFIVNVGDILEIMTNGIYR 293

Query: 485 SAVHRAVTNSEKQRISVACFCCPEKDREIEPIEGLIDERRPRLYR 527
           S  HRAV NS K+R+S+A F   + + EI PI  L+    P L++
Sbjct: 294 SVEHRAVVNSTKERLSIATFHDSKLESEIGPISSLVTPETPALFK 333

BLAST of MS002396 vs. ExPASy TrEMBL
Match: A0A6J1BYU1 (uncharacterized protein LOC111006992 OS=Momordica charantia OX=3673 GN=LOC111006992 PE=4 SV=1)

HSP 1 Score: 1016.1 bits (2626), Expect = 5.4e-293
Identity = 532/707 (75.25%), Postives = 537/707 (75.95%), Query Frame = 0

Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK
Sbjct: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60

Query: 61  LRHGLHSWGCFQVIS----------------VEFAL------------------------ 120
           LRHGLHSWGCFQ I+                + FAL                        
Sbjct: 61  LRHGLHSWGCFQAINHGMSPEFLEEVRQVAKLFFALPMEDKLKHSREEDKMEGYGNDMIF 120

Query: 121 ----------------------------LNP---RSGVRGYTANVKLLSEKILKAMARSL 180
                                        NP   R  +  YTANVKLLSEKILKAMARSL
Sbjct: 121 SNQQILDWTDRLYLTVCPEESRRFKYWPTNPERFREVLHEYTANVKLLSEKILKAMARSL 180

Query: 181 DLDENSFLNQYGERVQLDARFNFYPRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240
           DLDENSFLNQYG+RVQLDARFNFY RCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL
Sbjct: 181 DLDENSFLNQYGKRVQLDARFNFYLRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFL 240

Query: 241 KGNEWWNAPIIPDALLVNVGDQGE------------------------------------ 300
           KGNEWWNAPIIPDALLVNVGDQGE                                    
Sbjct: 241 KGNEWWNAPIIPDALLVNVGDQGEITSNGIFKSRVHRVLTNSERERISLAVFYLPDPQKE 300

Query: 301 -------------------------------------------------PSNMAELPDYF 360
                                                            PSNMAELPDYF
Sbjct: 301 IEPLEKLINETHPRLYRTVKNFVGLFFQYYQQGQRPREAAKIISVHLRKPSNMAELPDYF 360

Query: 361 HTKTLQQKLLINGGDTPESYIYKDGYGGGDSNNNPLPLAEIPVVDLAQLSSSPPSTAALE 420
           HTKT QQKLLINGGDTPESYIYKDGYGGGDSNNNPLPLAEIPVVDLAQLSSSPPSTAALE
Sbjct: 361 HTKTFQQKLLINGGDTPESYIYKDGYGGGDSNNNPLPLAEIPVVDLAQLSSSPPSTAALE 420

Query: 421 DLRLALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSLPMEEKNKCCRELYGLEGYGTD 480
           DLRLALTSWGCFQ   INHSI SSFLNKI QISNQFFSLPMEEKNKCCRELYGLEGYGTD
Sbjct: 421 DLRLALTSWGCFQ--AINHSISSSFLNKIHQISNQFFSLPMEEKNKCCRELYGLEGYGTD 480

Query: 481 MVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMA 540
           MVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMA
Sbjct: 481 MVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMA 540

Query: 541 RSINVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGL 552
           RSINVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGL
Sbjct: 541 RSINVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGL 600

BLAST of MS002396 vs. ExPASy TrEMBL
Match: A0A0A0LW96 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G212830 PE=4 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 4.8e-209
Identity = 371/567 (65.43%), Postives = 437/567 (77.07%), Query Frame = 0

Query: 1   MAGTNPTGSVQDVASKGEVPERYIHKECDRGALDAPLMEAPVIDIGLLSSPSNTGPELEK 60
           MA TNP+G+VQD ASKGEVPERYIHKE DRGA +AP M APVIDI LLSS S +GPELE+
Sbjct: 27  MAVTNPSGNVQDAASKGEVPERYIHKESDRGARNAPTMAAPVIDIALLSSSSKSGPELEE 86

Query: 61  LRHGLHSWGCFQV----------ISVEFALLNPRSGVRGYTANVKLLSEKILKAMARSLD 120
           LRH L SWGCFQ+          + + +   N +  +  YT NVKL+SEKI KAMARSLD
Sbjct: 87  LRHVLQSWGCFQIKLKIFINVCDVLISYKSWNDKEVIDEYTTNVKLISEKIFKAMARSLD 146

Query: 121 LDENSFLNQYGERVQLDARFNFYPRCRNPNLVLGVKPHADGSAITILLQDKEVEGLQFLK 180
           LDE+SFL QYGE+++L ARFNFYPRCRNP+LVLGVKPHADGSAITILLQDKEVEGLQF+K
Sbjct: 147 LDESSFLKQYGEQIKLGARFNFYPRCRNPDLVLGVKPHADGSAITILLQDKEVEGLQFMK 206

Query: 181 GNEWWNAPIIPDALLVNVGDQGEPSNMAELPDYFHTKTLQQKLLINGGDTPESYIYKDGY 240
            NEW+NA I+PDALLVNVGDQ E     +     ++K   + +    GD    +   D  
Sbjct: 207 DNEWFNASIVPDALLVNVGDQVE----VKEETLVYSKITVKHVCKQSGDLIVDHKALD-- 266

Query: 241 GGGDSNNNPLPLAEIPVVDLA------QLSSSPPSTAALEDLRLALTSWGCFQVSTINHS 300
                    L L    V  LA       L S       L DLRLAL++WGCFQ +  NHS
Sbjct: 267 --------DLELIHQVVSRLALKHNNRYLKSPSAGEGPLNDLRLALSTWGCFQAT--NHS 326

Query: 301 IPSSFLNKIRQISNQFFSLPMEEKNKCCRELYGLEGYGTDMVFSEQQILDWTDRLYLKVN 360
           I SSFL K+R+IS QFFSLP+EEK +  RE+ G+EGYG D+ FS QQ LDW+DRLY   +
Sbjct: 327 ISSSFLEKLRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLTFSNQQTLDWSDRLYFVTS 386

Query: 361 PEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMARSINVEANSFTEQVGKRPEL 420
           PEDER+L  WP NP SFREDLHE+T+K+ +IIETVL+AMARS+NVE NSFT+QVG+RP L
Sbjct: 387 PEDERRLDLWPLNPPSFREDLHEYTVKIMEIIETVLIAMARSLNVEPNSFTDQVGERPTL 446

Query: 421 FTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGLQWRKDDQWFRVPVPAMADSL 480
           FTRFNFYPPCS P LVLGLKEHSDGSAITI+LLD++VEGLQ RKDDQW+RVPVPA+ADSL
Sbjct: 447 FTRFNFYPPCSTPHLVLGLKEHSDGSAITILLLDKQVEGLQLRKDDQWYRVPVPAIADSL 506

Query: 481 LINIGEQAEIMSNGVFKSAVHRAVTNSEKQRISVACFCCPEKDREIEPIEGLIDERRPRL 540
           L+ IGEQAE+MSNG+FKS+VHRAVTNSE+QRISV CFCCPEKD EI+P+EGLIDE+RPRL
Sbjct: 507 LLVIGEQAEVMSNGIFKSSVHRAVTNSERQRISVVCFCCPEKDIEIKPVEGLIDEKRPRL 566

Query: 541 YRSVKNYVGSYFQDYQKGQRPVDKLKI 552
           +RSVKNY+ +YFQ+YQ+GQR VD L+I
Sbjct: 567 FRSVKNYLETYFQNYQEGQRTVDGLRI 577

BLAST of MS002396 vs. ExPASy TrEMBL
Match: A0A1S3CP07 (protein SRG1-like OS=Cucumis melo OX=3656 GN=LOC103503159 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.8e-147
Identity = 257/356 (72.19%), Postives = 306/356 (85.96%), Query Frame = 0

Query: 197 MAELPDYFHTKTLQQKLLINGGDTPESYIYKDGYGGGDSNNN-PLPLAEIPVVDLAQLSS 256
           MA+      T+TLQQ+LLINGG TPESYIYK GY GGDSNNN PLPLA+IPV+DL+QLSS
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 257 SPPSTAALEDLRLALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSLPMEEKNKCCREL 316
           +    A L  LRLAL++WGCFQ +  NH I SSFL K+R+IS QFFSLP+EEK +  RE+
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQAT--NHGISSSFLEKMRKISEQFFSLPIEEKMRYGREV 120

Query: 317 YGLEGYGTDMVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQI 376
            G+EGYG D++FS QQILDW+DRLYL  NP+DER+L++WP NP SFREDLHE+T+KL +I
Sbjct: 121 DGMEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFREDLHEYTVKLMKI 180

Query: 377 IETVLMAMARSINVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIV 436
           I+TVL+AMARS+NVE NSFT+QVGKRP LF RFNFYPPCS P LVLGLKEHSDG+AIT++
Sbjct: 181 IDTVLIAMARSLNVEPNSFTDQVGKRPTLFKRFNFYPPCSTPHLVLGLKEHSDGTAITVL 240

Query: 437 LLDREVEGLQWRKDDQWFRVPVPAMADSLLINIGEQAEIMSNGVFKSAVHRAVTNSEKQR 496
           LLD++VEGL+ RKDDQW+RVPVPA+ADSLLI IGEQAEIMSNG+FKS +HRAVTNSE+QR
Sbjct: 241 LLDKQVEGLELRKDDQWYRVPVPAVADSLLIIIGEQAEIMSNGIFKSLIHRAVTNSERQR 300

Query: 497 ISVACFCCPEKDREIEPIEGLIDERRPRLYRSVKNYVGSYFQDYQKGQRPVDKLKI 552
           ISV  FCCPEKD EI+PIEGLIDE+RPRL+RS KNY+ +YFQ+YQKG+R VD L+I
Sbjct: 301 ISVVSFCCPEKDIEIKPIEGLIDEKRPRLFRSTKNYLQTYFQNYQKGERSVDGLRI 354

BLAST of MS002396 vs. ExPASy TrEMBL
Match: A0A5D3CEG2 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00240 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 1.5e-146
Identity = 258/357 (72.27%), Postives = 306/357 (85.71%), Query Frame = 0

Query: 197 MAELPDYFHTKTLQQKLLINGGDTPESYIYKDGYGGGDSNNN-PLPLAEIPVVDLAQLSS 256
           MA+      T+TLQQ+LLINGG TPESYIYK GY GGDSNNN PLPLA+IPV+DL+QLSS
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 257 SPPSTAALEDLRLALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSLPMEEKNKCCREL 316
           +    A L  LRLAL++WGCFQ +  NH I SSFL K+R+IS QFFSLP+EEK +  RE+
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQAT--NHGISSSFLEKMRKISEQFFSLPIEEKMRYGREV 120

Query: 317 YGLEGYGTDMVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSF-REDLHEFTIKLKQ 376
            G+EGYG D++FS QQILDW+DRLYL  NP+DER+L++WP NP SF REDLHE+T+KL +
Sbjct: 121 DGMEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFSREDLHEYTVKLME 180

Query: 377 IIETVLMAMARSINVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITI 436
           IIETVL+AMARS+NVE NSFT+QVGKRP LF RFNFYPPCS P LVLGLKEHSDG+AIT+
Sbjct: 181 IIETVLIAMARSLNVEPNSFTDQVGKRPTLFKRFNFYPPCSTPHLVLGLKEHSDGTAITV 240

Query: 437 VLLDREVEGLQWRKDDQWFRVPVPAMADSLLINIGEQAEIMSNGVFKSAVHRAVTNSEKQ 496
           +LLD++VEGL+ RKDDQW+RVPVPA+ADSLLI IGEQAEIMSNG+FKS +HRAVTNSE+Q
Sbjct: 241 LLLDKQVEGLELRKDDQWYRVPVPAVADSLLIIIGEQAEIMSNGIFKSLIHRAVTNSERQ 300

Query: 497 RISVACFCCPEKDREIEPIEGLIDERRPRLYRSVKNYVGSYFQDYQKGQRPVDKLKI 552
           RISV  FCCPEKD EI+PIEGLIDE+RPRL+RS KNY+ +YFQ+YQKG+R VD L+I
Sbjct: 301 RISVVSFCCPEKDIEIKPIEGLIDEKRPRLFRSTKNYLQTYFQNYQKGERSVDGLRI 355

BLAST of MS002396 vs. ExPASy TrEMBL
Match: A0A5A7U2F3 (Protein SRG1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00670 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 9.8e-146
Identity = 257/357 (71.99%), Postives = 305/357 (85.43%), Query Frame = 0

Query: 197 MAELPDYFHTKTLQQKLLINGGDTPESYIYKDGYGGGDSNNN-PLPLAEIPVVDLAQLSS 256
           MA+      T+TLQQ+LLINGG TPESYIYK GY GGDSNNN PLPLA+IPV+DL+QLSS
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 257 SPPSTAALEDLRLALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSLPMEEKNKCCREL 316
           +    A L  LRLAL++WGCFQ +  NH I SSFL K+R+IS QFFSLP+EEK +  RE+
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQAT--NHGISSSFLEKMRKISEQFFSLPIEEKMRYGREV 120

Query: 317 YGLEGYGTDMVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSF-REDLHEFTIKLKQ 376
            G+EGYG D++FS QQILDW+DRLYL  NPED R+L++WP NP SF REDLHE+T+KL +
Sbjct: 121 DGMEGYGNDLIFSNQQILDWSDRLYLVTNPEDGRRLEFWPLNPPSFSREDLHEYTVKLME 180

Query: 377 IIETVLMAMARSINVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITI 436
           IIETVL+AMARS+NVE NSFT+QVGKRP LF RFNFYPPCS P LVLGLKEHSDG+AIT+
Sbjct: 181 IIETVLIAMARSLNVEPNSFTDQVGKRPTLFKRFNFYPPCSTPHLVLGLKEHSDGTAITV 240

Query: 437 VLLDREVEGLQWRKDDQWFRVPVPAMADSLLINIGEQAEIMSNGVFKSAVHRAVTNSEKQ 496
           +LLD++VEGL+ RKDDQW+RVPVPA+ADSLLI IGEQAEIMSNG+FKS +HRAVTNSE++
Sbjct: 241 LLLDKQVEGLELRKDDQWYRVPVPAVADSLLIIIGEQAEIMSNGIFKSLIHRAVTNSERR 300

Query: 497 RISVACFCCPEKDREIEPIEGLIDERRPRLYRSVKNYVGSYFQDYQKGQRPVDKLKI 552
           RISV  FCCPEKD EI+PIEGLIDE+RPRL+RS KNY+ +YFQ+YQKG+R VD L+I
Sbjct: 301 RISVVSFCCPEKDIEIKPIEGLIDEKRPRLFRSTKNYLQTYFQNYQKGERSVDGLRI 355

BLAST of MS002396 vs. TAIR 10
Match: AT5G20400.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 351.3 bits (900), Expect = 1.4e-96
Identity = 168/339 (49.56%), Postives = 236/339 (69.62%), Query Frame = 0

Query: 211 QKLLINGGDTPESYIY-KDGYGGGDSNNNPLPLAEIPVVDL-AQLSSSPPSTAALEDLRL 270
           Q+++  G   PE Y++   G G     N  +P  +IP +DL   LSSS      L  L  
Sbjct: 10  QEVVAAGEGLPERYLHAPTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAGQQELSKLHS 69

Query: 271 ALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSLPMEEKNKCCRELYGLEGYGTDMVFS 330
           AL++WG  QV  +NH I  +FL+KI +++ +FF+LP EEK KC RE+  ++GYG DM+  
Sbjct: 70  ALSTWGVVQV--MNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGYGNDMILW 129

Query: 331 EQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMARSIN 390
           + Q+LDW DRLY+   PED+RQL +WP+ P  FRE LHE+T+K + +IE    AMARS+ 
Sbjct: 130 DDQVLDWIDRLYITTYPEDQRQLNFWPEVPLGFRETLHEYTMKQRIVIEQFFKAMARSLE 189

Query: 391 VEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGLQWRK 450
           +E NSF +  G+   L TRFN YPPC  PD V+G+K H+DGSAIT++L D++V GLQ++K
Sbjct: 190 LEENSFLDMYGESATLDTRFNMYPPCPSPDKVIGVKPHADGSAITLLLPDKDVGGLQFQK 249

Query: 451 DDQWFRVPVPAMADSLLINIGEQAEIMSNGVFKSAVHRAVTNSEKQRISVACFCCPEKDR 510
           D +W++ P+  + D++LIN+G+Q EIMSNG++KS VHR VTN EK+RISVA FC P  D+
Sbjct: 250 DGKWYKAPI--VPDTILINVGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCIPGADK 309

Query: 511 EIEPIEGLIDERRPRLYRSVKNYVGSYFQDYQKGQRPVD 548
           EI+P+  L+ E RPRLY++VK YV  YF+ YQ+G+RP++
Sbjct: 310 EIQPVNELVSEARPRLYKTVKKYVELYFKYYQQGRRPIE 344

BLAST of MS002396 vs. TAIR 10
Match: AT1G49390.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 344.7 bits (883), Expect = 1.3e-94
Identity = 165/339 (48.67%), Postives = 236/339 (69.62%), Query Frame = 0

Query: 211 QKLLINGGDTPESYIY-KDGYGGGDSNNNPLPLAEIPVVDLAQL-SSSPPSTAALEDLRL 270
           Q+++  G   PE Y++   G G     N  +P  +IP +DL+ L SSS      ++ L  
Sbjct: 10  QEVVAAGQGLPERYLHAPTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEEMKKLHS 69

Query: 271 ALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSLPMEEKNKCCRELYGLEGYGTDMVFS 330
           AL++WG  QV  +NH I  +FL+KI +++ QFF+LP EEK+KC RE   ++GYG DM+ S
Sbjct: 70  ALSTWGVVQV--MNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGNDMILS 129

Query: 331 EQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMARSIN 390
           + Q+LDW DRL+L   PED+RQLK+WPQ P  F E L E+T+K + +IE    AMARS+ 
Sbjct: 130 DNQVLDWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDEYTMKQRVLIEKFFKAMARSLE 189

Query: 391 VEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGLQWRK 450
           +E N F E  G+   + +RFNF+PPC RPD V+G+K H+DGSAIT++L D++VEGLQ+ K
Sbjct: 190 LEENCFLEMYGENAVMNSRFNFFPPCPRPDKVIGIKPHADGSAITLLLPDKDVEGLQFLK 249

Query: 451 DDQWFRVPVPAMADSLLINIGEQAEIMSNGVFKSAVHRAVTNSEKQRISVACFCCPEKDR 510
           D +W++ P+  + D++LI +G+Q EIMSNG++KS VHR VTN EK+RISVA FC P  D+
Sbjct: 250 DGKWYKAPI--VPDTILITLGDQMEIMSNGIYKSPVHRVVTNREKERISVATFCVPGLDK 309

Query: 511 EIEPIEGLIDERRPRLYRSVKNYVGSYFQDYQKGQRPVD 548
           EI P +GL+ E RPRLY++V  YV  +++ YQ+G+R ++
Sbjct: 310 EIHPADGLVTEARPRLYKTVTKYVDLHYKYYQQGRRTIE 344

BLAST of MS002396 vs. TAIR 10
Match: AT5G54000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 337.0 bits (863), Expect = 2.8e-92
Identity = 164/344 (47.67%), Postives = 231/344 (67.15%), Query Frame = 0

Query: 211 QKLLINGGDTPESYIY-KDGYGGGDSN-NNPLPLAEIPVVDLAQL-SSSPPSTAALEDLR 270
           Q+++  G   PE Y+Y   G G GD   N  LP  +I ++DL  L SSS      L  L 
Sbjct: 10  QEVVAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGREELSKLH 69

Query: 271 LALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSLPMEEKNKCCRELYGLEGYGTDMVF 330
            A+++WG  QV  +NH I  + L+KI +++ QFF LP +EK K  RE+   +G+G DM+ 
Sbjct: 70  SAISTWGVVQV--MNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGNDMIL 129

Query: 331 SEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMARSI 390
           S+ Q+LDW DRLYL   PED+RQLK+WP+NP  FRE LHE+T+K + ++E    A+ARS+
Sbjct: 130 SDDQVLDWVDRLYLITYPEDQRQLKFWPENPSGFRETLHEYTMKQQLVVEKFFKALARSL 189

Query: 391 NVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGLQWR 450
            +E N F E  G+   L TRFN YPPC RPD VLGLK HSDGSA T++L D+ VEGLQ+ 
Sbjct: 190 ELEDNCFLEMHGENATLETRFNIYPPCPRPDKVLGLKPHSDGSAFTLILPDKNVEGLQFL 249

Query: 451 KDDQWFRVPVPAMADSLLINIGEQAEIMSNGVFKSAVHRAVTNSEKQRISVACFCCPEKD 510
           KD +W++  +  +  ++LIN+G+  E+MSNG++KS VHR V N +K+RI VA FC  ++D
Sbjct: 250 KDGKWYKASI--LPHTILINVGDTMEVMSNGIYKSPVHRVVLNGKKERIYVATFCNADED 309

Query: 511 REIEPIEGLIDERRPRLYRSVKNYVGSYFQDYQKGQRPVDKLKI 552
           +EI+P+ GL+ E RPRLY++VK    ++F  YQ+G+RP++   I
Sbjct: 310 KEIQPLNGLVSEARPRLYKAVKKSEKNFFDYYQQGRRPIEAAMI 349

BLAST of MS002396 vs. TAIR 10
Match: AT5G20550.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 332.8 bits (852), Expect = 5.2e-91
Identity = 162/338 (47.93%), Postives = 226/338 (66.86%), Query Frame = 0

Query: 211 QKLLINGGDTPESYIYKDGY-GGGDSNNNPLPLAEIPVVDLA-QLSSSPPSTAALEDLRL 270
           Q+++  G   PE Y+        G   N  +P+ +IP +DL+  LS S      L  L  
Sbjct: 10  QEVVAAGEGIPERYLQPPAVDDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREELSKLHS 69

Query: 271 ALTSWGCFQVSTINHSIPSSFLNKIRQISNQFFSLPMEEKNKCCRELYGLEGYGTDMVFS 330
           AL++WG  QV  INH I  + L+KI +++ +F +LP EEK K  RE+  ++GYG DM+  
Sbjct: 70  ALSTWGVVQV--INHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGNDMILW 129

Query: 331 EQQILDWTDRLYLKVNPEDERQLKYWPQNPQSFREDLHEFTIKLKQIIETVLMAMARSIN 390
           + Q+LDW DRLY+   PED+RQLK+WP  P  FRE LHE+T+K   +   V  AMA S+ 
Sbjct: 130 DDQVLDWIDRLYITTYPEDQRQLKFWPDVPVGFRETLHEYTMKQHLVFNQVFKAMAISLE 189

Query: 391 VEANSFTEQVGKRPELFTRFNFYPPCSRPDLVLGLKEHSDGSAITIVLLDREVEGLQWRK 450
           +E N F +  G+   + TRFN YPPC RPD V+G++ H+D SA T++L D+ VEGLQ+ K
Sbjct: 190 LEENCFLDMCGENATMDTRFNMYPPCPRPDKVIGVRPHADKSAFTLLLPDKNVEGLQFLK 249

Query: 451 DDQWFRVPVPAMADSLLINIGEQAEIMSNGVFKSAVHRAVTNSEKQRISVACFCCPEKDR 510
           D +W++ PV A +D++LIN+G+Q EIMSNG++KS VHR VTN+EK+RISVA FC P  D+
Sbjct: 250 DGKWYKAPVVA-SDTILINVGDQMEIMSNGIYKSPVHRVVTNTEKERISVATFCIPGADK 309

Query: 511 EIEPIEGLIDERRPRLYRSVKNYVGSYFQDYQKGQRPV 547
           EI+P++GL+ E RPRLY+ VKNYV    + Y +GQRP+
Sbjct: 310 EIQPVDGLVSEARPRLYKPVKNYVDLLNKYYIQGQRPI 344

BLAST of MS002396 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 225.3 bits (573), Expect = 1.2e-58
Identity = 125/314 (39.81%), Postives = 185/314 (58.92%), Query Frame = 0

Query: 244 EIPVVDLAQLSSSPPSTAALEDLRL--ALTSWGCFQVSTINHSIPSSFLNKIRQISNQFF 303
           +IPV+DL++LS         E L+L  A   WG FQV  INH I    +  I +++++FF
Sbjct: 54  QIPVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQV--INHGIEVEVVEDIEEVASEFF 113

Query: 304 SLPMEEKNKCCRELYGLEGYGTDMVFSEQQILDWTDRLYLKVNPEDERQLKYWPQNPQSF 363
            +P+EEK K   E   ++GYG   +FSE Q LDW +   L V+P   R  K WP  P  F
Sbjct: 114 DMPLEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARF 173

Query: 364 REDLHEFTIKLKQIIETVLMAMARSINVEANSFTEQVGKRPELFTRFNFYPPCSRPDLVL 423
            E L  ++ +++++ + +L  +A S+ ++   F E  G+  +   R N+YPPCS PDLVL
Sbjct: 174 SESLEGYSKEIRELCKRLLKYIAISLGLKEERFEEMFGEAVQA-VRMNYYPPCSSPDLVL 233

Query: 424 GLKEHSDGSAITIVLLDR-EVEGLQWRKDDQWFRVPVPAMADSLLINIGEQAEIMSNGVF 483
           GL  HSDGSA+T++   +    GLQ  KD+ W  VPV  + ++L+INIG+  E++SNG +
Sbjct: 234 GLSPHSDGSALTVLQQSKNSCVGLQILKDNTW--VPVKPLPNALVINIGDTIEVLSNGKY 293

Query: 484 KSAVHRAVTNSEKQRISVACFCCPEKDREIEPIEGLI-DERRPRLYRSVK--NYVGSYFQ 543
           KS  HRAVTN EK+R+++  F  P  + EIEP+  L+ DE  P  YRS    +Y   Y  
Sbjct: 294 KSVEHRAVTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDYSYHYVS 353

Query: 544 DYQKGQRPVDKLKI 552
           +  +G++ +D  KI
Sbjct: 354 NKLQGKKSLDFAKI 362