MS002380 (gene) Bitter gourd (TR) v1

Overview
NameMS002380
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionE3 ubiquitin-protein ligase RNF12-A-like isoform X1
Locationscaffold30: 4487271 .. 4489632 (-)
RNA-Seq ExpressionMS002380
SyntenyMS002380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGATTCTACAGCTCAATTTCCCGTCCATTCGACAGAACATTGTAGTCACAATATTGATGATGTTCAAAGCCTCACAAGTCGATCTTACTCGATCTCCAGTACGAGAACGGGACTTCCTAGTGTAAGCACTAATTCTCAGAATGCATATGAGAATGGCATTGCAGTCGGTGAGGAGCAATGTGCTTTTCATGATCCAAGAAATAATGATGTCAATAGTCATGGGAAGTGTTTGTCTCAGAATAAGGAATCAGCTCCTTTCCAACAGGTTAGTGCTGATTACAGTCATGATGAATCTCATAGAAATAGTAATACTACCAATGGTACTTCATTTAAAGGACGTCAATCTTCGGAACCAGTCTCTGTAAATATTTTGGCTAACGAAGATGCAGTAAATGGCATTGATAGTTCAGTGGACAAGAGTGCATCTCCAGTCTGCACAAAGGTCATGCTTACAGCAAGCAGTTCGACTACTCAAGAGGTCGGAGACTTGCGTTCCAGTGAGTTGACAGGTTATATTCAAGAGGACGAGGCGACAGCTTACTGCAACCCGGACTCTGAAGCTTGTCCTTGTGGTTCTGATCTTCCAGTAACTTCTCAGTCTCTTGGAGATGAATCTTCTCAAGAGGTACACTCAAGTTTAGAATCTCTCGTGTCTGATGGTAGGGAACAAGGCCAAGAAGATGTGGGTATGCCTCTTGTTGATATGGTCGATATTTCTTCCAATGTTTTATCTACGACTAGCACTGATGCAAGTTATATTGAGGCCAGAAGAAATAGCAGAAGACTGTTTTGGGATGCCTTCTCACGACGTAGTTTTAGAAGGCATAGTGAATCTCCGTTTCTTTTCCCATCTAATAACATTAGTAATACAGGAGATACAGACCGATGGCTCCTCAATATAAGTGGTGATTTTATGGATGATGAAGTGGGTGCCGATTCAAGACATTTTGCCAGCAGAATTCACATCTTGAATGAGCAACAAAGAAACTCAAGATCTCAGGTTGCATTCTGAATGTTAACAGTTATGTTTTTTTTTTTTTAATCTACAATATCTCGTTCTTGAAAATGCAGAGTACACATACCGTGATCCTCTGTTAGAAGCAACATATAACTGAGATCAGCGTCATTTAGCACTCTACCAAACTGAAATGTTCTAGTGTTGTCACTTTAAGCAATAATTGATTCTATTTTCAACCTTCCATTGCGTTGTGTGCAGATATGGGAAAGACTTCGTAGTAGTCTCAGTGAAAACAGTCAGCAAAATTCATCTTGCCCATCAGGCATTCACCCTGACGGTGCTTGCACGTGTGACTCATTTTCAAGGATCGAACACTCGAGTACCCGTGCAAGCATATCACGAATAATCATGCTTGCTGAAGCATTATTTGAGGTAGTTTTCCCCTGCTCCATTCATTGAACTTTTGGATGATATTATGTTATGCAAACCATACATCACCAATATGTTGGTGTAGGCAAAGAATGTATATTGGCTGTTCATTCACTTCAACTGGAATTCTATGCTATCAATTAATTTAGTGAATTGGGTGTTTCAGATAAACGCTTTTGCACTCAAGTATTGCCTACTTTTTGGAGTTCTTGTGCTGCATTTCAACAGTTATCTCACATCTTGCTTTCTTTCTGTATCTGAAGGTCTTGGATGAAATTCATGGTCAGCCTGTTTCTCTTGCCCTATCCATGGTTTCACTCCCAGCACCAGAGTCAGTTGTTGACTCTTTTCCTCTGAAGAACCACGAAAAAACGACAAATGGAGGGGATGATATTGAGCAGTATGTTTCTTCTTTCAATCAAATAATAGTATTGTTACTGAGCATTAATATGGCATGTGATACAAGCCTCTTATCAAGTGTGTTTACAAACATTCCAATGCGATGTTTTAGTTAGCAATCACCTCTCTCTTATTGTATTGACTAATGCGATGCTTGCTTTAAGAGAAGGTACGAGACTCGTGGAACCGGAATGTTTTAAATTCGAACATTTACTTGTTTGTAGTTGTGCTTTTTAGCCTCTAATTTGGAAATGCTTTAAAATATTTGATTAGATGCTACATTTGCCTGGCTGAATACGAAGACGGTGACAAAATAAGAGTTCTTCCTTGTCGCCATGAATATCATATGTCATGTGTTGATAAATGGTTGAAGGAGATTCATGGGTATGTCATCTATACTTCGATTACCATTCGTTCTTTTACCTACATCGTGTCCTGCAATCTTTTCATTCATCAACTCATCTCTCCCTGTCTCGCTGTGTTTAACAGAGTATGCCCACTTTGTAGAGGTGATGTTCGTGCAGGCTCAAACGACGAGTGTTCGGTTTCAAACCCAGAAATTCCAACAATT

mRNA sequence

ATGGAAGATTCTACAGCTCAATTTCCCGTCCATTCGACAGAACATTGTAGTCACAATATTGATGATGTTCAAAGCCTCACAAGTCGATCTTACTCGATCTCCAGTACGAGAACGGGACTTCCTAGTGTAAGCACTAATTCTCAGAATGCATATGAGAATGGCATTGCAGTCGGTGAGGAGCAATGTGCTTTTCATGATCCAAGAAATAATGATGTCAATAGTCATGGGAAGTGTTTGTCTCAGAATAAGGAATCAGCTCCTTTCCAACAGGTTAGTGCTGATTACAGTCATGATGAATCTCATAGAAATAGTAATACTACCAATGGTACTTCATTTAAAGGACGTCAATCTTCGGAACCAGTCTCTGTAAATATTTTGGCTAACGAAGATGCAGTAAATGGCATTGATAGTTCAGTGGACAAGAGTGCATCTCCAGTCTGCACAAAGGTCATGCTTACAGCAAGCAGTTCGACTACTCAAGAGGTCGGAGACTTGCGTTCCAGTGAGTTGACAGGTTATATTCAAGAGGACGAGGCGACAGCTTACTGCAACCCGGACTCTGAAGCTTGTCCTTGTGGTTCTGATCTTCCAGTAACTTCTCAGTCTCTTGGAGATGAATCTTCTCAAGAGGTACACTCAAGTTTAGAATCTCTCGTGTCTGATGGTAGGGAACAAGGCCAAGAAGATGTGGGTATGCCTCTTGTTGATATGGTCGATATTTCTTCCAATGTTTTATCTACGACTAGCACTGATGCAAGTTATATTGAGGCCAGAAGAAATAGCAGAAGACTGTTTTGGGATGCCTTCTCACGACGTAGTTTTAGAAGGCATAGTGAATCTCCGTTTCTTTTCCCATCTAATAACATTAGTAATACAGGAGATACAGACCGATGGCTCCTCAATATAAGTGGTGATTTTATGGATGATGAAGTGGGTGCCGATTCAAGACATTTTGCCAGCAGAATTCACATCTTGAATGAGCAACAAAGAAACTCAAGATCTCAGATATGGGAAAGACTTCGTAGTAGTCTCAGTGAAAACAGTCAGCAAAATTCATCTTGCCCATCAGGCATTCACCCTGACGGTGCTTGCACGTGTGACTCATTTTCAAGGATCGAACACTCGAGTACCCGTGCAAGCATATCACGAATAATCATGCTTGCTGAAGCATTATTTGAGGTAGTCTTGGATGAAATTCATGGTCAGCCTGTTTCTCTTGCCCTATCCATGGTTTCACTCCCAGCACCAGAGTCAGTTGTTGACTCTTTTCCTCTGAAGAACCACGAAAAAACGACAAATGGAGGGGATGATATTGAGCAATGCTACATTTGCCTGGCTGAATACGAAGACGGTGACAAAATAAGAGTTCTTCCTTGTCGCCATGAATATCATATGTCATGTGTTGATAAATGGTTGAAGGAGATTCATGGAGTATGCCCACTTTGTAGAGGTGATGTTCGTGCAGGCTCAAACGACGAGTGTTCGGTTTCAAACCCAGAAATTCCAACAATT

Coding sequence (CDS)

ATGGAAGATTCTACAGCTCAATTTCCCGTCCATTCGACAGAACATTGTAGTCACAATATTGATGATGTTCAAAGCCTCACAAGTCGATCTTACTCGATCTCCAGTACGAGAACGGGACTTCCTAGTGTAAGCACTAATTCTCAGAATGCATATGAGAATGGCATTGCAGTCGGTGAGGAGCAATGTGCTTTTCATGATCCAAGAAATAATGATGTCAATAGTCATGGGAAGTGTTTGTCTCAGAATAAGGAATCAGCTCCTTTCCAACAGGTTAGTGCTGATTACAGTCATGATGAATCTCATAGAAATAGTAATACTACCAATGGTACTTCATTTAAAGGACGTCAATCTTCGGAACCAGTCTCTGTAAATATTTTGGCTAACGAAGATGCAGTAAATGGCATTGATAGTTCAGTGGACAAGAGTGCATCTCCAGTCTGCACAAAGGTCATGCTTACAGCAAGCAGTTCGACTACTCAAGAGGTCGGAGACTTGCGTTCCAGTGAGTTGACAGGTTATATTCAAGAGGACGAGGCGACAGCTTACTGCAACCCGGACTCTGAAGCTTGTCCTTGTGGTTCTGATCTTCCAGTAACTTCTCAGTCTCTTGGAGATGAATCTTCTCAAGAGGTACACTCAAGTTTAGAATCTCTCGTGTCTGATGGTAGGGAACAAGGCCAAGAAGATGTGGGTATGCCTCTTGTTGATATGGTCGATATTTCTTCCAATGTTTTATCTACGACTAGCACTGATGCAAGTTATATTGAGGCCAGAAGAAATAGCAGAAGACTGTTTTGGGATGCCTTCTCACGACGTAGTTTTAGAAGGCATAGTGAATCTCCGTTTCTTTTCCCATCTAATAACATTAGTAATACAGGAGATACAGACCGATGGCTCCTCAATATAAGTGGTGATTTTATGGATGATGAAGTGGGTGCCGATTCAAGACATTTTGCCAGCAGAATTCACATCTTGAATGAGCAACAAAGAAACTCAAGATCTCAGATATGGGAAAGACTTCGTAGTAGTCTCAGTGAAAACAGTCAGCAAAATTCATCTTGCCCATCAGGCATTCACCCTGACGGTGCTTGCACGTGTGACTCATTTTCAAGGATCGAACACTCGAGTACCCGTGCAAGCATATCACGAATAATCATGCTTGCTGAAGCATTATTTGAGGTAGTCTTGGATGAAATTCATGGTCAGCCTGTTTCTCTTGCCCTATCCATGGTTTCACTCCCAGCACCAGAGTCAGTTGTTGACTCTTTTCCTCTGAAGAACCACGAAAAAACGACAAATGGAGGGGATGATATTGAGCAATGCTACATTTGCCTGGCTGAATACGAAGACGGTGACAAAATAAGAGTTCTTCCTTGTCGCCATGAATATCATATGTCATGTGTTGATAAATGGTTGAAGGAGATTCATGGAGTATGCCCACTTTGTAGAGGTGATGTTCGTGCAGGCTCAAACGACGAGTGTTCGGTTTCAAACCCAGAAATTCCAACAATT

Protein sequence

MEDSTAQFPVHSTEHCSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVGEEQCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSSEPVSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDEATAYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMVDISSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRWLLNISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGIHPDGACTCDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAPESVVDSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCPLCRGDVRAGSNDECSVSNPEIPTI
Homology
BLAST of MS002380 vs. NCBI nr
Match: XP_022135152.1 (E3 ubiquitin ligase BIG BROTHER-related-like isoform X2 [Momordica charantia])

HSP 1 Score: 988.4 bits (2554), Expect = 2.3e-284
Identity = 503/504 (99.80%), Postives = 503/504 (99.80%), Query Frame = 0

Query: 1   MEDSTAQFPVHSTEHCSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVGEE 60
           MEDSTAQFPVHSTEHCSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVGEE
Sbjct: 1   MEDSTAQFPVHSTEHCSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVGEE 60

Query: 61  QCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSSEP 120
           QCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSSEP
Sbjct: 61  QCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSSEP 120

Query: 121 VSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDEAT 180
           VSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDEAT
Sbjct: 121 VSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDEAT 180

Query: 181 AYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMVDI 240
           AYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMVDI
Sbjct: 181 AYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMVDI 240

Query: 241 SSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRWLL 300
           SSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRWLL
Sbjct: 241 SSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRWLL 300

Query: 301 NISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGIHP 360
           NISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGIHP
Sbjct: 301 NISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGIHP 360

Query: 361 DGACTCDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAPESVV 420
           DGACTCDSFSRIEHSSTRASISRIIMLAEALFE VLDEIHGQPVSLALSMVSLPAPESVV
Sbjct: 361 DGACTCDSFSRIEHSSTRASISRIIMLAEALFE-VLDEIHGQPVSLALSMVSLPAPESVV 420

Query: 421 DSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCP 480
           DSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCP
Sbjct: 421 DSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCP 480

Query: 481 LCRGDVRAGSNDECSVSNPEIPTI 505
           LCRGDVRAGSNDECSVSNPEIPTI
Sbjct: 481 LCRGDVRAGSNDECSVSNPEIPTI 503

BLAST of MS002380 vs. NCBI nr
Match: XP_022135151.1 (uncharacterized protein LOC111007190 isoform X1 [Momordica charantia])

HSP 1 Score: 988.4 bits (2554), Expect = 2.3e-284
Identity = 503/504 (99.80%), Postives = 503/504 (99.80%), Query Frame = 0

Query: 1   MEDSTAQFPVHSTEHCSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVGEE 60
           MEDSTAQFPVHSTEHCSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVGEE
Sbjct: 41  MEDSTAQFPVHSTEHCSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVGEE 100

Query: 61  QCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSSEP 120
           QCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSSEP
Sbjct: 101 QCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSSEP 160

Query: 121 VSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDEAT 180
           VSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDEAT
Sbjct: 161 VSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDEAT 220

Query: 181 AYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMVDI 240
           AYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMVDI
Sbjct: 221 AYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMVDI 280

Query: 241 SSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRWLL 300
           SSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRWLL
Sbjct: 281 SSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRWLL 340

Query: 301 NISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGIHP 360
           NISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGIHP
Sbjct: 341 NISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGIHP 400

Query: 361 DGACTCDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAPESVV 420
           DGACTCDSFSRIEHSSTRASISRIIMLAEALFE VLDEIHGQPVSLALSMVSLPAPESVV
Sbjct: 401 DGACTCDSFSRIEHSSTRASISRIIMLAEALFE-VLDEIHGQPVSLALSMVSLPAPESVV 460

Query: 421 DSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCP 480
           DSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCP
Sbjct: 461 DSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCP 520

Query: 481 LCRGDVRAGSNDECSVSNPEIPTI 505
           LCRGDVRAGSNDECSVSNPEIPTI
Sbjct: 521 LCRGDVRAGSNDECSVSNPEIPTI 543

BLAST of MS002380 vs. NCBI nr
Match: XP_038876907.1 (uncharacterized protein LOC120069259 isoform X2 [Benincasa hispida])

HSP 1 Score: 785.8 bits (2028), Expect = 2.2e-223
Identity = 414/507 (81.66%), Postives = 439/507 (86.59%), Query Frame = 0

Query: 1   MEDSTAQFPVHSTEH--CSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVG 60
           MEDST QF VH TEH  C+HNID+VQ  T+      S R GLPS S  S+ +YEN IAV 
Sbjct: 41  MEDSTVQFLVHPTEHQQCNHNIDEVQLPTN-----GSIRLGLPSASAYSETSYENHIAVS 100

Query: 61  EEQCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSS 120
           EE+CA  DPRN ++N HGKCLSQNKE  P QQVSADYSHDESHR SN +NGTSFKG QSS
Sbjct: 101 EERCAVDDPRNININVHGKCLSQNKELTPLQQVSADYSHDESHRYSNASNGTSFKGHQSS 160

Query: 121 EPVSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDE 180
           EPVSVNILANEDAVNGIDSSVDK ASPVCTKVM  ASSSTTQE GD  S+ELTG IQEDE
Sbjct: 161 EPVSVNILANEDAVNGIDSSVDKGASPVCTKVMFAASSSTTQEAGDSCSNELTGSIQEDE 220

Query: 181 ATAYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLE-SLVSDGREQGQEDVGMPLVDM 240
            T Y  PDSE  P GS LP+T QSLGDESSQEV SSL+ +LVSD R+ GQEDVGMP VDM
Sbjct: 221 VTVYQVPDSEWSPSGSGLPLTFQSLGDESSQEVPSSLDITLVSDNRDLGQEDVGMPHVDM 280

Query: 241 VDISSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDR 300
           V ISSNVLST+STDAS +E RRNSRRLFWDAFSRRSFRR SESPF+FPSNN  NTGD DR
Sbjct: 281 VGISSNVLSTSSTDASSLEVRRNSRRLFWDAFSRRSFRRQSESPFVFPSNNFRNTGDPDR 340

Query: 301 WLLNISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSG 360
           WLL+ISGDF+DDEV  DSRHF+SRIHILNEQQRNSRSQIWERLRSSLSENS+QN SCPSG
Sbjct: 341 WLLDISGDFIDDEVSGDSRHFSSRIHILNEQQRNSRSQIWERLRSSLSENSRQNLSCPSG 400

Query: 361 IHPDGACTCDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAPE 420
           IHPDG C+CDSFSRIEHSSTRASISRIIMLAEALFE VLDEIHGQPVSLALSMVSLPAPE
Sbjct: 401 IHPDGPCSCDSFSRIEHSSTRASISRIIMLAEALFE-VLDEIHGQPVSLALSMVSLPAPE 460

Query: 421 SVVDSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHG 480
           SVVDSFPLKNHEKTTNGGD+IEQCYICLAEYE+GDKIRVLPCRHEYHM CVDKWLKEIHG
Sbjct: 461 SVVDSFPLKNHEKTTNGGDEIEQCYICLAEYEEGDKIRVLPCRHEYHMLCVDKWLKEIHG 520

Query: 481 VCPLCRGDVRAGSNDECSVSNPEIPTI 505
           VCPLCRGDVRAGSN ECSV N E+PTI
Sbjct: 521 VCPLCRGDVRAGSNTECSVPNSEVPTI 541

BLAST of MS002380 vs. NCBI nr
Match: XP_038876906.1 (uncharacterized protein LOC120069259 isoform X1 [Benincasa hispida])

HSP 1 Score: 780.8 bits (2015), Expect = 7.1e-222
Identity = 414/509 (81.34%), Postives = 439/509 (86.25%), Query Frame = 0

Query: 1   MEDSTAQFPVHSTEH--CSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVG 60
           MEDST QF VH TEH  C+HNID+VQ  T+      S R GLPS S  S+ +YEN IAV 
Sbjct: 41  MEDSTVQFLVHPTEHQQCNHNIDEVQLPTN-----GSIRLGLPSASAYSETSYENHIAVS 100

Query: 61  EEQCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSS 120
           EE+CA  DPRN ++N HGKCLSQNKE  P QQVSADYSHDESHR SN +NGTSFKG QSS
Sbjct: 101 EERCAVDDPRNININVHGKCLSQNKELTPLQQVSADYSHDESHRYSNASNGTSFKGHQSS 160

Query: 121 EPVSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDE 180
           EPVSVNILANEDAVNGIDSSVDK ASPVCTKVM  ASSSTTQE GD  S+ELTG IQEDE
Sbjct: 161 EPVSVNILANEDAVNGIDSSVDKGASPVCTKVMFAASSSTTQEAGDSCSNELTGSIQEDE 220

Query: 181 ATAYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLE-SLVSDGREQGQEDVGMPLVDM 240
            T Y  PDSE  P GS LP+T QSLGDESSQEV SSL+ +LVSD R+ GQEDVGMP VDM
Sbjct: 221 VTVYQVPDSEWSPSGSGLPLTFQSLGDESSQEVPSSLDITLVSDNRDLGQEDVGMPHVDM 280

Query: 241 VDISSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDR 300
           V ISSNVLST+STDAS +E RRNSRRLFWDAFSRRSFRR SESPF+FPSNN  NTGD DR
Sbjct: 281 VGISSNVLSTSSTDASSLEVRRNSRRLFWDAFSRRSFRRQSESPFVFPSNNFRNTGDPDR 340

Query: 301 WLLNISGDFMDDEVGADSRHFASRIHILNEQQRNSRS--QIWERLRSSLSENSQQNSSCP 360
           WLL+ISGDF+DDEV  DSRHF+SRIHILNEQQRNSRS  QIWERLRSSLSENS+QN SCP
Sbjct: 341 WLLDISGDFIDDEVSGDSRHFSSRIHILNEQQRNSRSQMQIWERLRSSLSENSRQNLSCP 400

Query: 361 SGIHPDGACTCDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPA 420
           SGIHPDG C+CDSFSRIEHSSTRASISRIIMLAEALFE VLDEIHGQPVSLALSMVSLPA
Sbjct: 401 SGIHPDGPCSCDSFSRIEHSSTRASISRIIMLAEALFE-VLDEIHGQPVSLALSMVSLPA 460

Query: 421 PESVVDSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEI 480
           PESVVDSFPLKNHEKTTNGGD+IEQCYICLAEYE+GDKIRVLPCRHEYHM CVDKWLKEI
Sbjct: 461 PESVVDSFPLKNHEKTTNGGDEIEQCYICLAEYEEGDKIRVLPCRHEYHMLCVDKWLKEI 520

Query: 481 HGVCPLCRGDVRAGSNDECSVSNPEIPTI 505
           HGVCPLCRGDVRAGSN ECSV N E+PTI
Sbjct: 521 HGVCPLCRGDVRAGSNTECSVPNSEVPTI 543

BLAST of MS002380 vs. NCBI nr
Match: KAA0050044.1 (E3 ubiquitin-protein ligase RNF12-A-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 748.0 bits (1930), Expect = 5.1e-212
Identity = 399/508 (78.54%), Postives = 429/508 (84.45%), Query Frame = 0

Query: 1   MEDSTAQFPVHSTEH--CSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVG 60
           MEDS+ QF VH T+H  C+HNID+VQ  T+      S R GLP+ S  S+ +YENGIA  
Sbjct: 1   MEDSSVQFLVHPTDHQQCNHNIDEVQLPTN-----GSIRMGLPNASAYSETSYENGIADS 60

Query: 61  EEQCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSS 120
           EE  A  DPRN D+N HGKCL QNKE AP QQVSADYS DESHR SN +NGTSFKG QSS
Sbjct: 61  EE-IAVDDPRNIDINVHGKCLFQNKELAPPQQVSADYSPDESHRYSNASNGTSFKGHQSS 120

Query: 121 EPVSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDE 180
           EPVSVNILANEDAVNGID SVDK  SPVC+KV   ASSSTTQEVG+L SS LTG IQEDE
Sbjct: 121 EPVSVNILANEDAVNGIDGSVDKGVSPVCSKVTFAASSSTTQEVGNLCSSGLTGSIQEDE 180

Query: 181 ATAYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMV 240
            +    P SE+ P GS LP+T QSL DESSQE  SSL++L SD RE GQ D+GMP VDMV
Sbjct: 181 VSVNQGPFSESSPSGSGLPLTFQSLEDESSQEEPSSLDTLDSDNRELGQGDLGMPHVDMV 240

Query: 241 DISSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRW 300
            ISSNVLST+STD S +E RRNSRRLFWDAFSRRSFRR  ESPF+FPSNN SNTGDTDRW
Sbjct: 241 GISSNVLSTSSTDTSSLEVRRNSRRLFWDAFSRRSFRRQRESPFVFPSNNFSNTGDTDRW 300

Query: 301 LLNISGDFMDDEVGADSRHFASRIHILNEQQRNSRS--QIWERLRSSLSENSQQNSSCPS 360
           LL+ISGDF+DDEV  DSRHF+SRIHILNEQQRNSRS  QIWERLRSSL+E SQQN SCPS
Sbjct: 301 LLDISGDFIDDEVSGDSRHFSSRIHILNEQQRNSRSQVQIWERLRSSLTETSQQNVSCPS 360

Query: 361 GIHPDGACTCDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAP 420
           GIHP+G C+CDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAP
Sbjct: 361 GIHPNGPCSCDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAP 420

Query: 421 ESVVDSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIH 480
           ESVVDSFPLKNHEKTTNGGD+IEQCYICLAEYE+GDKIRVLPCRHEYHM CVDKWLKEIH
Sbjct: 421 ESVVDSFPLKNHEKTTNGGDEIEQCYICLAEYEEGDKIRVLPCRHEYHMLCVDKWLKEIH 480

Query: 481 GVCPLCRGDVRAGSNDECSVSNPEIPTI 505
           GVCPLCRGDVRAGSNDECSV N E+ TI
Sbjct: 481 GVCPLCRGDVRAGSNDECSVPNNEVSTI 502

BLAST of MS002380 vs. ExPASy Swiss-Prot
Match: Q91XF4 (E3 ubiquitin-protein ligase RNF167 OS=Mus musculus OX=10090 GN=Rnf167 PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 2.5e-15
Identity = 33/59 (55.93%), Postives = 39/59 (66.10%), Query Frame = 0

Query: 435 GDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCPLCRGDVRAGSNDE 494
           GD+ + C ICL EYEDGDK+RVLPC H YH  CVD WL +    CP+C+  V  G  DE
Sbjct: 224 GDEYDVCAICLDEYEDGDKLRVLPCAHAYHSRCVDPWLTQTRKTCPICKQPVHRGPGDE 282

BLAST of MS002380 vs. ExPASy Swiss-Prot
Match: Q5XIL0 (E3 ubiquitin-protein ligase RNF167 OS=Rattus norvegicus OX=10116 GN=Rnf167 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 3.2e-15
Identity = 32/59 (54.24%), Postives = 39/59 (66.10%), Query Frame = 0

Query: 435 GDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCPLCRGDVRAGSNDE 494
           GD+ + C ICL EYEDGDK+R+LPC H YH  CVD WL +    CP+C+  V  G  DE
Sbjct: 224 GDEYDVCAICLDEYEDGDKLRILPCAHAYHSRCVDPWLTQTRKTCPICKQPVHRGPGDE 282

BLAST of MS002380 vs. ExPASy Swiss-Prot
Match: Q9H6Y7 (E3 ubiquitin-protein ligase RNF167 OS=Homo sapiens OX=9606 GN=RNF167 PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 4.2e-15
Identity = 33/59 (55.93%), Postives = 38/59 (64.41%), Query Frame = 0

Query: 435 GDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCPLCRGDVRAGSNDE 494
           GD  + C ICL EYEDGDK+RVLPC H YH  CVD WL +    CP+C+  V  G  DE
Sbjct: 224 GDQYDVCAICLDEYEDGDKLRVLPCAHAYHSRCVDPWLTQTRKTCPICKQPVHRGPGDE 282

BLAST of MS002380 vs. ExPASy Swiss-Prot
Match: O54965 (E3 ubiquitin-protein ligase RNF13 OS=Mus musculus OX=10090 GN=Rnf13 PE=1 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 1.3e-13
Identity = 31/61 (50.82%), Postives = 38/61 (62.30%), Query Frame = 0

Query: 435 GDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCPLCRGDVRAGSNDEC 494
           GD+ + C ICL EYEDGDK+R+LPC H YH  CVD WL +    CP+C+  V     D  
Sbjct: 234 GDEYDVCAICLEEYEDGDKLRILPCSHAYHCKCVDPWLTKTKKTCPVCKQKVVPSQGDSD 293

Query: 495 S 496
           S
Sbjct: 294 S 294

BLAST of MS002380 vs. ExPASy Swiss-Prot
Match: Q66HG0 (E3 ubiquitin-protein ligase RNF13 OS=Rattus norvegicus OX=10116 GN=Rnf13 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.3e-13
Identity = 31/61 (50.82%), Postives = 38/61 (62.30%), Query Frame = 0

Query: 435 GDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCPLCRGDVRAGSNDEC 494
           GD+ + C ICL EYEDGDK+R+LPC H YH  CVD WL +    CP+C+  V     D  
Sbjct: 234 GDEYDVCAICLEEYEDGDKLRILPCSHAYHCKCVDPWLTKTKKTCPVCKQKVVPSQGDSD 293

Query: 495 S 496
           S
Sbjct: 294 S 294

BLAST of MS002380 vs. ExPASy TrEMBL
Match: A0A6J1BZU1 (uncharacterized protein LOC111007190 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007190 PE=4 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 1.1e-284
Identity = 503/504 (99.80%), Postives = 503/504 (99.80%), Query Frame = 0

Query: 1   MEDSTAQFPVHSTEHCSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVGEE 60
           MEDSTAQFPVHSTEHCSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVGEE
Sbjct: 41  MEDSTAQFPVHSTEHCSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVGEE 100

Query: 61  QCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSSEP 120
           QCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSSEP
Sbjct: 101 QCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSSEP 160

Query: 121 VSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDEAT 180
           VSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDEAT
Sbjct: 161 VSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDEAT 220

Query: 181 AYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMVDI 240
           AYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMVDI
Sbjct: 221 AYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMVDI 280

Query: 241 SSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRWLL 300
           SSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRWLL
Sbjct: 281 SSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRWLL 340

Query: 301 NISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGIHP 360
           NISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGIHP
Sbjct: 341 NISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGIHP 400

Query: 361 DGACTCDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAPESVV 420
           DGACTCDSFSRIEHSSTRASISRIIMLAEALFE VLDEIHGQPVSLALSMVSLPAPESVV
Sbjct: 401 DGACTCDSFSRIEHSSTRASISRIIMLAEALFE-VLDEIHGQPVSLALSMVSLPAPESVV 460

Query: 421 DSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCP 480
           DSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCP
Sbjct: 461 DSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCP 520

Query: 481 LCRGDVRAGSNDECSVSNPEIPTI 505
           LCRGDVRAGSNDECSVSNPEIPTI
Sbjct: 521 LCRGDVRAGSNDECSVSNPEIPTI 543

BLAST of MS002380 vs. ExPASy TrEMBL
Match: A0A6J1C1V5 (E3 ubiquitin ligase BIG BROTHER-related-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007190 PE=4 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 1.1e-284
Identity = 503/504 (99.80%), Postives = 503/504 (99.80%), Query Frame = 0

Query: 1   MEDSTAQFPVHSTEHCSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVGEE 60
           MEDSTAQFPVHSTEHCSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVGEE
Sbjct: 1   MEDSTAQFPVHSTEHCSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVGEE 60

Query: 61  QCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSSEP 120
           QCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSSEP
Sbjct: 61  QCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSSEP 120

Query: 121 VSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDEAT 180
           VSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDEAT
Sbjct: 121 VSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDEAT 180

Query: 181 AYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMVDI 240
           AYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMVDI
Sbjct: 181 AYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMVDI 240

Query: 241 SSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRWLL 300
           SSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRWLL
Sbjct: 241 SSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRWLL 300

Query: 301 NISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGIHP 360
           NISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGIHP
Sbjct: 301 NISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGIHP 360

Query: 361 DGACTCDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAPESVV 420
           DGACTCDSFSRIEHSSTRASISRIIMLAEALFE VLDEIHGQPVSLALSMVSLPAPESVV
Sbjct: 361 DGACTCDSFSRIEHSSTRASISRIIMLAEALFE-VLDEIHGQPVSLALSMVSLPAPESVV 420

Query: 421 DSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCP 480
           DSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCP
Sbjct: 421 DSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCP 480

Query: 481 LCRGDVRAGSNDECSVSNPEIPTI 505
           LCRGDVRAGSNDECSVSNPEIPTI
Sbjct: 481 LCRGDVRAGSNDECSVSNPEIPTI 503

BLAST of MS002380 vs. ExPASy TrEMBL
Match: A0A5A7U484 (E3 ubiquitin-protein ligase RNF12-A-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00230 PE=4 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 2.5e-212
Identity = 399/508 (78.54%), Postives = 429/508 (84.45%), Query Frame = 0

Query: 1   MEDSTAQFPVHSTEH--CSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVG 60
           MEDS+ QF VH T+H  C+HNID+VQ  T+      S R GLP+ S  S+ +YENGIA  
Sbjct: 1   MEDSSVQFLVHPTDHQQCNHNIDEVQLPTN-----GSIRMGLPNASAYSETSYENGIADS 60

Query: 61  EEQCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSS 120
           EE  A  DPRN D+N HGKCL QNKE AP QQVSADYS DESHR SN +NGTSFKG QSS
Sbjct: 61  EE-IAVDDPRNIDINVHGKCLFQNKELAPPQQVSADYSPDESHRYSNASNGTSFKGHQSS 120

Query: 121 EPVSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDE 180
           EPVSVNILANEDAVNGID SVDK  SPVC+KV   ASSSTTQEVG+L SS LTG IQEDE
Sbjct: 121 EPVSVNILANEDAVNGIDGSVDKGVSPVCSKVTFAASSSTTQEVGNLCSSGLTGSIQEDE 180

Query: 181 ATAYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMV 240
            +    P SE+ P GS LP+T QSL DESSQE  SSL++L SD RE GQ D+GMP VDMV
Sbjct: 181 VSVNQGPFSESSPSGSGLPLTFQSLEDESSQEEPSSLDTLDSDNRELGQGDLGMPHVDMV 240

Query: 241 DISSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRW 300
            ISSNVLST+STD S +E RRNSRRLFWDAFSRRSFRR  ESPF+FPSNN SNTGDTDRW
Sbjct: 241 GISSNVLSTSSTDTSSLEVRRNSRRLFWDAFSRRSFRRQRESPFVFPSNNFSNTGDTDRW 300

Query: 301 LLNISGDFMDDEVGADSRHFASRIHILNEQQRNSRS--QIWERLRSSLSENSQQNSSCPS 360
           LL+ISGDF+DDEV  DSRHF+SRIHILNEQQRNSRS  QIWERLRSSL+E SQQN SCPS
Sbjct: 301 LLDISGDFIDDEVSGDSRHFSSRIHILNEQQRNSRSQVQIWERLRSSLTETSQQNVSCPS 360

Query: 361 GIHPDGACTCDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAP 420
           GIHP+G C+CDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAP
Sbjct: 361 GIHPNGPCSCDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAP 420

Query: 421 ESVVDSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIH 480
           ESVVDSFPLKNHEKTTNGGD+IEQCYICLAEYE+GDKIRVLPCRHEYHM CVDKWLKEIH
Sbjct: 421 ESVVDSFPLKNHEKTTNGGDEIEQCYICLAEYEEGDKIRVLPCRHEYHMLCVDKWLKEIH 480

Query: 481 GVCPLCRGDVRAGSNDECSVSNPEIPTI 505
           GVCPLCRGDVRAGSNDECSV N E+ TI
Sbjct: 481 GVCPLCRGDVRAGSNDECSVPNNEVSTI 502

BLAST of MS002380 vs. ExPASy TrEMBL
Match: A0A1S3B977 (uncharacterized protein LOC103487388 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487388 PE=4 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 4.2e-212
Identity = 398/506 (78.66%), Postives = 428/506 (84.58%), Query Frame = 0

Query: 1   MEDSTAQFPVHSTEH--CSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVG 60
           MEDS+ QF VH T+H  C+HNID+VQ  T+      S R GLP+ S  S+ +YENGIA  
Sbjct: 41  MEDSSVQFLVHPTDHQQCNHNIDEVQLPTN-----GSIRMGLPNASAYSETSYENGIADS 100

Query: 61  EEQCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSS 120
           EE  A  DPRN D+N HGKCL QNKE AP QQVSADYS DESHR SN +NGTSFKG QSS
Sbjct: 101 EES-AVDDPRNIDINVHGKCLFQNKELAPPQQVSADYSPDESHRYSNASNGTSFKGHQSS 160

Query: 121 EPVSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDE 180
           EPVSVNILANEDAVNGID SVDK  SPVC+KV   ASSSTTQEVG+L SS LTG IQEDE
Sbjct: 161 EPVSVNILANEDAVNGIDGSVDKGVSPVCSKVTFAASSSTTQEVGNLCSSGLTGSIQEDE 220

Query: 181 ATAYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMV 240
            +    P SE+ P GS LP+T QSL DESSQE  SSL++L SD RE GQ D+GMP VDMV
Sbjct: 221 VSVNQGPFSESSPSGSGLPLTFQSLEDESSQEEPSSLDTLDSDNRELGQGDLGMPHVDMV 280

Query: 241 DISSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRW 300
            ISSNVLST+STD S +E RRNSRRLFWDAFSRRSFRR  ESPF+FPSNN SNTGDTDRW
Sbjct: 281 GISSNVLSTSSTDTSSLEVRRNSRRLFWDAFSRRSFRRQRESPFVFPSNNFSNTGDTDRW 340

Query: 301 LLNISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGI 360
           LL+ISGDF+DDEV  DSRHF+SRIHILNEQQRNSRSQIWERLRSSL+E SQQN SCPSGI
Sbjct: 341 LLDISGDFIDDEVSGDSRHFSSRIHILNEQQRNSRSQIWERLRSSLTETSQQNVSCPSGI 400

Query: 361 HPDGACTCDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAPES 420
           HP+G C+CDSFSRIEHSSTRASISRIIMLAEALFE VLDEIHGQPVSLALSMVSLPAPES
Sbjct: 401 HPNGPCSCDSFSRIEHSSTRASISRIIMLAEALFE-VLDEIHGQPVSLALSMVSLPAPES 460

Query: 421 VVDSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGV 480
           VVDSFPLKNHEKTTNGGD+IEQCYICLAEYE+GDKIRVLPCRHEYHM CVDKWLKEIHGV
Sbjct: 461 VVDSFPLKNHEKTTNGGDEIEQCYICLAEYEEGDKIRVLPCRHEYHMLCVDKWLKEIHGV 520

Query: 481 CPLCRGDVRAGSNDECSVSNPEIPTI 505
           CPLCRGDVRAGSNDECSV N E+ TI
Sbjct: 521 CPLCRGDVRAGSNDECSVPNNEVSTI 539

BLAST of MS002380 vs. ExPASy TrEMBL
Match: A0A0A0LTU1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G265080 PE=4 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 4.7e-211
Identity = 397/507 (78.30%), Postives = 427/507 (84.22%), Query Frame = 0

Query: 1   MEDSTAQFPVHSTEH--CSHNIDDVQSLTSRSYSISSTRTGLPSVSTNSQNAYENGIAVG 60
           MEDS+ QF VH TEH  C+HNID+VQ  T+      S R  LP+ ST S+ +YENGIA G
Sbjct: 41  MEDSSVQFLVHPTEHQQCNHNIDEVQLPTN-----GSIRMELPNASTYSETSYENGIADG 100

Query: 61  EEQCAFHDPRNNDVNSHGKCLSQNKESAPFQQVSADYSHDESHRNSNTTNGTSFKGRQSS 120
           EE+ A  DPRN D+N HGKCL QNKE A  QQVSADYS DESHR SN +NGTSFKG QSS
Sbjct: 101 EERTAVDDPRNIDINVHGKCLFQNKELASPQQVSADYSPDESHRYSNASNGTSFKGHQSS 160

Query: 121 EPVSVNILANEDAVNGIDSSVDKSASPVCTKVMLTASSSTTQEVGDLRSSELTGYIQEDE 180
           EPVSVNILANEDAVNGID SVDK  SPVCTKVM  ASSSTTQEVG+  SS LTG IQEDE
Sbjct: 161 EPVSVNILANEDAVNGIDGSVDKDISPVCTKVMFAASSSTTQEVGNSCSSGLTGSIQEDE 220

Query: 181 ATAYCNPDSEACPCGSDLPVTSQSLGDESSQEVHSSLESLVSDGREQGQEDVGMPLVDMV 240
            +    P  E+ P GS LP+T QSLGDESSQE  S L++L SD RE    DVG+P VDMV
Sbjct: 221 VSVIQGPFLESSPSGSGLPLTFQSLGDESSQEEPSILDTLESDDRELDHGDVGLPHVDMV 280

Query: 241 DISSNVLSTTSTDASYIEARRNSRRLFWDAFSRRSFRRHSESPFLFPSNNISNTGDTDRW 300
            ISSNVLST+STD S +E RRNSRRLFWDAFSRRSFRR  ESPF+FPSNN SNTGDTDRW
Sbjct: 281 GISSNVLSTSSTDTSSLEVRRNSRRLFWDAFSRRSFRRQRESPFVFPSNNFSNTGDTDRW 340

Query: 301 LLNISGDFMDDEVGADSRHFASRIHILNEQQRNSRSQIWERLRSSLSENSQQNSSCPSGI 360
           LL+ISGDF+DDEV  DSRHF+SRIHILNEQQRNSRSQIWERLRSSL+E SQQN SCPSGI
Sbjct: 341 LLDISGDFIDDEVSGDSRHFSSRIHILNEQQRNSRSQIWERLRSSLTETSQQNVSCPSGI 400

Query: 361 HPDGACTCDSFSRIEHSSTRASISRIIMLAEALFEVVLDEIHGQPVSLALSMVSLPAPES 420
           HP+G C+CDSFSRIEHSSTRASISRIIMLAEALFE VLDEIHGQPVSLALSMVSLPAPE+
Sbjct: 401 HPNGPCSCDSFSRIEHSSTRASISRIIMLAEALFE-VLDEIHGQPVSLALSMVSLPAPEA 460

Query: 421 VVDSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGV 480
           VVDSFPLKNHEKTTNGGD+IEQCYICLAEYE+GDKIRVLPCRHEYHM CVDKWLKEIHGV
Sbjct: 461 VVDSFPLKNHEKTTNGGDEIEQCYICLAEYEEGDKIRVLPCRHEYHMLCVDKWLKEIHGV 520

Query: 481 CPLCRGDVRAGSNDECS-VSNPEIPTI 505
           CPLCRGDVRAGSNDECS V N E+PTI
Sbjct: 521 CPLCRGDVRAGSNDECSVVPNNEVPTI 541

BLAST of MS002380 vs. TAIR 10
Match: AT5G45290.1 (RING/U-box superfamily protein )

HSP 1 Score: 145.2 bits (365), Expect = 1.4e-34
Identity = 87/177 (49.15%), Postives = 112/177 (63.28%), Query Frame = 0

Query: 327 EQQRNSRSQI--WERLRSSLSENS-QQNSSCP-SGIHPDGACTC-------DSFSRIEHS 386
           E++R  RSQ+   +RL S     +   + SC  SG    G CTC        + +  + +
Sbjct: 362 ERRRRIRSQVRALQRLGSRFENVAVHHDRSCVLSGQDQAGRCTCRAVTNRGSTTATTDET 421

Query: 387 STRASISRIIMLAEALFEVVLDEIHGQPVSLA------LSMVSLPAPESVVDSFPLKNHE 446
           + RASISRI++LAEALFE VLDEIH Q V L+       S+ S+PAP  VVD  P+K + 
Sbjct: 422 NARASISRIVLLAEALFE-VLDEIHQQSVVLSSQQPSVSSIGSVPAPNDVVDLLPIKLYT 481

Query: 447 KTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCPLCRGDV 487
           K+ +  +D  QCYICL EYE+ D IR LPC HE+H +CVDKWLKEIH VCPLCRGD+
Sbjct: 482 KSQS--EDPSQCYICLVEYEEADSIRTLPCHHEFHKTCVDKWLKEIHRVCPLCRGDI 535

BLAST of MS002380 vs. TAIR 10
Match: AT5G45290.2 (RING/U-box superfamily protein )

HSP 1 Score: 141.4 bits (355), Expect = 2.0e-33
Identity = 87/178 (48.88%), Postives = 112/178 (62.92%), Query Frame = 0

Query: 327 EQQRNSRSQI--WERLRSSLSENS-QQNSSCP-SGIHPDGACTC-------DSFSRIEHS 386
           E++R  RSQ+   +RL S     +   + SC  SG    G CTC        + +  + +
Sbjct: 362 ERRRRIRSQVRALQRLGSRFENVAVHHDRSCVLSGQDQAGRCTCRAVTNRGSTTATTDET 421

Query: 387 STRASISRIIMLAEALFEVVLDEIHGQPVSLA------LSMVSLPAPESVVDSFPLKNHE 446
           + RASISRI++LAEALFE VLDEIH Q V L+       S+ S+PAP  VVD  P+K + 
Sbjct: 422 NARASISRIVLLAEALFE-VLDEIHQQSVVLSSQQPSVSSIGSVPAPNDVVDLLPIKLYT 481

Query: 447 KTTNGGDDIEQCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHG-VCPLCRGDV 487
           K+ +  +D  QCYICL EYE+ D IR LPC HE+H +CVDKWLKEIH  VCPLCRGD+
Sbjct: 482 KSQS--EDPSQCYICLVEYEEADSIRTLPCHHEFHKTCVDKWLKEIHSRVCPLCRGDI 536

BLAST of MS002380 vs. TAIR 10
Match: AT4G09560.1 (Protease-associated (PA) RING/U-box zinc finger family protein )

HSP 1 Score: 75.1 bits (183), Expect = 1.8e-13
Identity = 27/53 (50.94%), Postives = 38/53 (71.70%), Query Frame = 0

Query: 441 CYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCPLCRGDVRAGSNDE 494
           C ICL  YE GDK+R+LPC H++H++CVD WL +    CP+C+ D R+ S D+
Sbjct: 234 CCICLENYEKGDKLRILPCHHKFHVACVDLWLGQRKSFCPVCKRDARSISTDK 286

BLAST of MS002380 vs. TAIR 10
Match: AT4G11680.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 72.0 bits (175), Expect = 1.5e-12
Identity = 27/54 (50.00%), Postives = 39/54 (72.22%), Query Frame = 0

Query: 440 QCYICLAEYEDGDKIRVLPCRHEYHMSCVDKWLKEIHGVCPLCRGDVRAGSNDE 494
           +C ICL EYEDG ++R LPC H +H +C+DKWL  I+  CPLC+ ++   +N+E
Sbjct: 337 ECCICLCEYEDGVELRELPCNHHFHCTCIDKWL-HINSRCPLCKFNILKNANNE 389

BLAST of MS002380 vs. TAIR 10
Match: AT1G71980.1 (Protease-associated (PA) RING/U-box zinc finger family protein )

HSP 1 Score: 71.2 bits (173), Expect = 2.6e-12
Identity = 36/95 (37.89%), Postives = 48/95 (50.53%), Query Frame = 0

Query: 398 EIHGQPVSLALSMVSLPAPESVVDSFPLKNHEKTTNGGDDIEQCYICLAEYEDGDKIRVL 457
           E HG    L  +M SL     +  SF    HE  T        C ICL +Y  GDK+R+L
Sbjct: 202 EFHGMSRRLVKAMPSL-----IFSSF----HEDNTTA----FTCAICLEDYTVGDKLRLL 261

Query: 458 PCRHEYHMSCVDKWLKEIHGVCPLCRGDVRAGSND 493
           PC H++H +CVD WL      CP+C+ D R  + +
Sbjct: 262 PCCHKFHAACVDSWLTSWRTFCPVCKRDARTSTGE 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135152.12.3e-28499.80E3 ubiquitin ligase BIG BROTHER-related-like isoform X2 [Momordica charantia][more]
XP_022135151.12.3e-28499.80uncharacterized protein LOC111007190 isoform X1 [Momordica charantia][more]
XP_038876907.12.2e-22381.66uncharacterized protein LOC120069259 isoform X2 [Benincasa hispida][more]
XP_038876906.17.1e-22281.34uncharacterized protein LOC120069259 isoform X1 [Benincasa hispida][more]
KAA0050044.15.1e-21278.54E3 ubiquitin-protein ligase RNF12-A-like isoform X1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q91XF42.5e-1555.93E3 ubiquitin-protein ligase RNF167 OS=Mus musculus OX=10090 GN=Rnf167 PE=2 SV=1[more]
Q5XIL03.2e-1554.24E3 ubiquitin-protein ligase RNF167 OS=Rattus norvegicus OX=10116 GN=Rnf167 PE=2 ... [more]
Q9H6Y74.2e-1555.93E3 ubiquitin-protein ligase RNF167 OS=Homo sapiens OX=9606 GN=RNF167 PE=1 SV=1[more]
O549651.3e-1350.82E3 ubiquitin-protein ligase RNF13 OS=Mus musculus OX=10090 GN=Rnf13 PE=1 SV=2[more]
Q66HG01.3e-1350.82E3 ubiquitin-protein ligase RNF13 OS=Rattus norvegicus OX=10116 GN=Rnf13 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1BZU11.1e-28499.80uncharacterized protein LOC111007190 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1C1V51.1e-28499.80E3 ubiquitin ligase BIG BROTHER-related-like isoform X2 OS=Momordica charantia O... [more]
A0A5A7U4842.5e-21278.54E3 ubiquitin-protein ligase RNF12-A-like isoform X1 OS=Cucumis melo var. makuwa ... [more]
A0A1S3B9774.2e-21278.66uncharacterized protein LOC103487388 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0LTU14.7e-21178.30RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G265080 P... [more]
Match NameE-valueIdentityDescription
AT5G45290.11.4e-3449.15RING/U-box superfamily protein [more]
AT5G45290.22.0e-3348.88RING/U-box superfamily protein [more]
AT4G09560.11.8e-1350.94Protease-associated (PA) RING/U-box zinc finger family protein [more]
AT4G11680.11.5e-1250.00Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G71980.12.6e-1237.89Protease-associated (PA) RING/U-box zinc finger family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 441..482
e-value: 1.8E-6
score: 37.5
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 440..483
e-value: 2.0E-11
score: 44.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 441..483
score: 12.54829
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 407..489
e-value: 2.6E-20
score: 73.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..118
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..94
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 102..118
NoneNo IPR availablePANTHERPTHR47168RING ZINC FINGER DOMAIN SUPERFAMILY PROTEIN-RELATEDcoord: 1..502
NoneNo IPR availablePANTHERPTHR47168:SF1RING ZINC FINGER DOMAIN SUPERFAMILY PROTEIN-RELATEDcoord: 1..502
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 430..487

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002380.1MS002380.1mRNA