MS002351 (gene) Bitter gourd (TR) v1

Overview
NameMS002351
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionReplication termination factor 2
Locationscaffold30: 4331487 .. 4332644 (+)
RNA-Seq ExpressionMS002351
SyntenyMS002351
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATCCGAAAAATCAACAACGTCTTCAGATCTTCGTTCATTCTCCCGACCTCCAAATTCAATCAAAAATCCTAGACCTAGCACAAACTTCCGCCCAAACCCTAGAGGATCTTAAATTCTCTCTTCTCCCGCAAACGCTTGCTCCACGGTTTGCGTCATCTTTCTACTTCACCTTGAATGGAAAACCCCTCCTTGATTCCACCCCAGTTACCAGTTCTCTAATTTCCCCTCTGTCCACCTTAATTTTAAGAACCAGGATCCATGGCGGTGGTGGTGACGGCGGTGCAACCGGGGCCGAATCCCGTGACTGCTACCTTAATATGTACGCAGAGAAGAAGCCCGATAAGGTCGACCCAAACGAGCAGAGGCTGTCCAAGTGGTTGAACTGCGCCCTTTCTAACGAGCCTTTGAGGGAACCTTGCGTGATCGATTGGCTCGGAAATGTCTTTAATAAGGAGTCGCTCGTGCAGGCTTTGCTAGAGAAGAAGCTGCCAAAGGGATTCGGGCATGTTAAGGGTCTGAAGGATATGATCAAGATAAGTCTTTCGGCAATTCCTGGTACAGAATCGGGTAGCAATGCGATTTCAGGTCCACGGTTTCAGTGCCCGATTACTGGGCTCGAGTTCAATGGTAAGTACAAGTTTTTTGCTCTGAGAACTTGTGGACACGTTCTGAGTGCAAAGGCCTTGAAGGAGGTTAAATCTTCCTCATGCCTTGTCTGTCATGCTGAGTTTAAGGACAGAGACAAGTTAGTGATCAATGGGAGCGAGGAGGAGGTAGCAGTACTGAGAGAGAGGATGGAGGAAGAGAAATCAAAATCAAAGGAGAAGAGGCTGAAGAAAGTGAAGAATGGGGAAGTGGGGATGAATGGAGATGTGAGTGTGGATTTGGCGTTATCCCGTTTGTCTGGTAAAAAGCATGGTATTGAAGTTAAGGCATCGGAGAAGGTTTCAGCTAAGCTTGAAAGGAATGAGCGGCCGGATGGTGGTATTCAGGTAAAGGTTTCAGCAAATAATGGTGCTGTAAAGCGATTCAAAGCAGCAGACATGGCCCCTCCCAATGCTACCAAAGAAGTCTATGCTTCGATATTCACCTCATCCAGGAAGTCAGATTTTAAGGAAACGTATTCTTGTAGATCTCTTCCACTGGGTCGGAAC

mRNA sequence

ATGCATCCGAAAAATCAACAACGTCTTCAGATCTTCGTTCATTCTCCCGACCTCCAAATTCAATCAAAAATCCTAGACCTAGCACAAACTTCCGCCCAAACCCTAGAGGATCTTAAATTCTCTCTTCTCCCGCAAACGCTTGCTCCACGGTTTGCGTCATCTTTCTACTTCACCTTGAATGGAAAACCCCTCCTTGATTCCACCCCAGTTACCAGTTCTCTAATTTCCCCTCTGTCCACCTTAATTTTAAGAACCAGGATCCATGGCGGTGGTGGTGACGGCGGTGCAACCGGGGCCGAATCCCGTGACTGCTACCTTAATATGTACGCAGAGAAGAAGCCCGATAAGGTCGACCCAAACGAGCAGAGGCTGTCCAAGTGGTTGAACTGCGCCCTTTCTAACGAGCCTTTGAGGGAACCTTGCGTGATCGATTGGCTCGGAAATGTCTTTAATAAGGAGTCGCTCGTGCAGGCTTTGCTAGAGAAGAAGCTGCCAAAGGGATTCGGGCATGTTAAGGGTCTGAAGGATATGATCAAGATAAGTCTTTCGGCAATTCCTGGTACAGAATCGGGTAGCAATGCGATTTCAGGTCCACGGTTTCAGTGCCCGATTACTGGGCTCGAGTTCAATGGTAAGTACAAGTTTTTTGCTCTGAGAACTTGTGGACACGTTCTGAGTGCAAAGGCCTTGAAGGAGGTTAAATCTTCCTCATGCCTTGTCTGTCATGCTGAGTTTAAGGACAGAGACAAGTTAGTGATCAATGGGAGCGAGGAGGAGGTAGCAGTACTGAGAGAGAGGATGGAGGAAGAGAAATCAAAATCAAAGGAGAAGAGGCTGAAGAAAGTGAAGAATGGGGAAGTGGGGATGAATGGAGATGTGAGTGTGGATTTGGCGTTATCCCGTTTGTCTGGTAAAAAGCATGGTATTGAAGTTAAGGCATCGGAGAAGGTTTCAGCTAAGCTTGAAAGGAATGAGCGGCCGGATGGTGGTATTCAGGTAAAGGTTTCAGCAAATAATGGTGCTGTAAAGCGATTCAAAGCAGCAGACATGGCCCCTCCCAATGCTACCAAAGAAGTCTATGCTTCGATATTCACCTCATCCAGGAAGTCAGATTTTAAGGAAACGTATTCTTGTAGATCTCTTCCACTGGGTCGGAAC

Coding sequence (CDS)

ATGCATCCGAAAAATCAACAACGTCTTCAGATCTTCGTTCATTCTCCCGACCTCCAAATTCAATCAAAAATCCTAGACCTAGCACAAACTTCCGCCCAAACCCTAGAGGATCTTAAATTCTCTCTTCTCCCGCAAACGCTTGCTCCACGGTTTGCGTCATCTTTCTACTTCACCTTGAATGGAAAACCCCTCCTTGATTCCACCCCAGTTACCAGTTCTCTAATTTCCCCTCTGTCCACCTTAATTTTAAGAACCAGGATCCATGGCGGTGGTGGTGACGGCGGTGCAACCGGGGCCGAATCCCGTGACTGCTACCTTAATATGTACGCAGAGAAGAAGCCCGATAAGGTCGACCCAAACGAGCAGAGGCTGTCCAAGTGGTTGAACTGCGCCCTTTCTAACGAGCCTTTGAGGGAACCTTGCGTGATCGATTGGCTCGGAAATGTCTTTAATAAGGAGTCGCTCGTGCAGGCTTTGCTAGAGAAGAAGCTGCCAAAGGGATTCGGGCATGTTAAGGGTCTGAAGGATATGATCAAGATAAGTCTTTCGGCAATTCCTGGTACAGAATCGGGTAGCAATGCGATTTCAGGTCCACGGTTTCAGTGCCCGATTACTGGGCTCGAGTTCAATGGTAAGTACAAGTTTTTTGCTCTGAGAACTTGTGGACACGTTCTGAGTGCAAAGGCCTTGAAGGAGGTTAAATCTTCCTCATGCCTTGTCTGTCATGCTGAGTTTAAGGACAGAGACAAGTTAGTGATCAATGGGAGCGAGGAGGAGGTAGCAGTACTGAGAGAGAGGATGGAGGAAGAGAAATCAAAATCAAAGGAGAAGAGGCTGAAGAAAGTGAAGAATGGGGAAGTGGGGATGAATGGAGATGTGAGTGTGGATTTGGCGTTATCCCGTTTGTCTGGTAAAAAGCATGGTATTGAAGTTAAGGCATCGGAGAAGGTTTCAGCTAAGCTTGAAAGGAATGAGCGGCCGGATGGTGGTATTCAGGTAAAGGTTTCAGCAAATAATGGTGCTGTAAAGCGATTCAAAGCAGCAGACATGGCCCCTCCCAATGCTACCAAAGAAGTCTATGCTTCGATATTCACCTCATCCAGGAAGTCAGATTTTAAGGAAACGTATTCTTGTAGATCTCTTCCACTGGGTCGGAAC

Protein sequence

MHPKNQQRLQIFVHSPDLQIQSKILDLAQTSAQTLEDLKFSLLPQTLAPRFASSFYFTLNGKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKISLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLVCHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLALSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVSANNGAVKRFKAADMAPPNATKEVYASIFTSSRKSDFKETYSCRSLPLGRN
Homology
BLAST of MS002351 vs. NCBI nr
Match: XP_022135450.1 (protein RTF2 homolog [Momordica charantia])

HSP 1 Score: 745.7 bits (1924), Expect = 2.0e-211
Identity = 381/386 (98.70%), Postives = 384/386 (99.48%), Query Frame = 0

Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILDLAQTSAQTLEDLKFSLLPQTLAPRFASSFYFTLN 60
           MHPKNQQRLQIFVHSPDLQIQSKIL+LA+TSAQTLEDLKFSLLPQTLAPR ASSFYFTLN
Sbjct: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLALS 300
           CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLA S
Sbjct: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVSANNGAVKRFKAADMAPPNATKEVY 360
           RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKV+ANNGAVKRFKAADMAPPNATKEVY
Sbjct: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY 360

Query: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 387
           ASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of MS002351 vs. NCBI nr
Match: XP_038879920.1 (replication termination factor 2 [Benincasa hispida])

HSP 1 Score: 655.6 bits (1690), Expect = 2.7e-184
Identity = 335/388 (86.34%), Postives = 357/388 (92.01%), Query Frame = 0

Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILDLAQTSAQTLEDLKFSLLPQTLAPRFASSFYFTLN 60
           MHPKN+ RLQIF+HS DLQIQS+I++L Q+ AQTLEDLKFSLLP+TLA R ASSFYFTLN
Sbjct: 1   MHPKNEHRLQIFLHSLDLQIQSQIVNLPQSPAQTLEDLKFSLLPETLASRVASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL DST + SSL+ PLSTLILRTR+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLPDSTTIPSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLV ALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           +LS IPGTES  NAIS PRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSVIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSK--SKEKRLKKVKNGEVGMNGDVSVDLA 300
           CHAEF +RDK VINGSEEEV  +RERMEEEKSK  SKEK+ KKV+N E G+NGD+SVD A
Sbjct: 241 CHAEFTNRDKFVINGSEEEVEEMRERMEEEKSKSRSKEKKTKKVRNEEGGINGDMSVDFA 300

Query: 301 LSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVSANNGAVKRFKAADMAPPNATKE 360
            SRLSGKKHGIEVKA EKVSAK ER+ERPDGG+QVKV+A+NGAVKRFKAADM P NATKE
Sbjct: 301 ASRLSGKKHGIEVKALEKVSAKPERHERPDGGVQVKVAASNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 387
           VYASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 388

BLAST of MS002351 vs. NCBI nr
Match: XP_008444038.1 (PREDICTED: protein RTF2 homolog [Cucumis melo] >KAA0035058.1 protein RTF2-like protein [Cucumis melo var. makuwa] >TYK03620.1 protein RTF2-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 652.5 bits (1682), Expect = 2.2e-183
Identity = 337/388 (86.86%), Postives = 355/388 (91.49%), Query Frame = 0

Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILDLAQTSAQTLEDLKFSLLPQTLAPRFASSFYFTLN 60
           MHPKNQ RLQIF+HSPDLQIQSKI+DL QTSA+TLEDLKFSLL +TLA R ASS YFTLN
Sbjct: 1   MHPKNQHRLQIFLHSPDLQIQSKIVDLPQTSAKTLEDLKFSLLSETLASRVASSLYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDST  T SLI PLSTLILRTR+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDST--TISLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPL EPCVIDWLGNVFNKESLVQALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLSEPCVIDWLGNVFNKESLVQALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           + S IPGTES  NAIS PRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NFSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEE--KSKSKEKRLKKVKNGEVGMNGDVSVDLA 300
           CH EF +RDK VINGSEEEV  +RERMEEE  KSKSKEK+ KKV+NGEVGMNGDV++DLA
Sbjct: 241 CHTEFTERDKFVINGSEEEVEEMRERMEEEKSKSKSKEKKTKKVRNGEVGMNGDVTMDLA 300

Query: 301 LSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVSANNGAVKRFKAADMAPPNATKE 360
            SRLSGKKHGIE++  EKVSAK ER+ER DGG QVKV+A+NGAVKRFKAADM P NATKE
Sbjct: 301 TSRLSGKKHGIELQTLEKVSAKPERHERLDGGAQVKVAASNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 387
           VYASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of MS002351 vs. NCBI nr
Match: XP_022933589.1 (protein RTF2 homolog [Cucurbita moschata])

HSP 1 Score: 649.8 bits (1675), Expect = 1.5e-182
Identity = 330/386 (85.49%), Postives = 352/386 (91.19%), Query Frame = 0

Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILDLAQTSAQTLEDLKFSLLPQTLAPRFASSFYFTLN 60
           M+PKNQ RLQ FVHSPDLQIQSKI++LAQTSAQ+L DLK SL+  T A R ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDS  ++SSL+ PLSTLILRTR+ GGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGN+FNKE LV ALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           +LS +PGTES  NAIS  RFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMVPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLALS 300
           CHAEF DRDK VINGSEEEV  +RERMEEEK+KSKEK+ KKV+ GEVGMNGDVSVDLA S
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVSANNGAVKRFKAADMAPPNATKEVY 360
           RLSGKKHGIE KA  KVSAK E++ERPD G+QVKV+A+NGAVKRFKAADMAP NATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKPEKHERPDSGVQVKVAASNGAVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 387
           ASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of MS002351 vs. NCBI nr
Match: KAG7035670.1 (Protein RTF2-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 649.4 bits (1674), Expect = 1.9e-182
Identity = 331/386 (85.75%), Postives = 351/386 (90.93%), Query Frame = 0

Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILDLAQTSAQTLEDLKFSLLPQTLAPRFASSFYFTLN 60
           M+PKNQ RLQ FVHSPDLQIQSKI++LAQTSAQ+L DLK SL+  T A R ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDS  ++SSL+ PLSTLILRTR+ GGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGN+FNKE LV ALLEKKLPKGFGHVKGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHVKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           +LS IPGTES  NAIS  RFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMIPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLALS 300
           CHAEF DRDK VINGSEEEV  +RERMEEEK+KSKEK+ KKV+ GEVGMNGDVSVDLA S
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVSANNGAVKRFKAADMAPPNATKEVY 360
           RLSGKKHGIE KA  KVSAK E++ERPD G+QVKV+A+NG VKRFKAADMAP NATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKSEKHERPDSGVQVKVAASNGGVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 387
           ASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of MS002351 vs. ExPASy Swiss-Prot
Match: Q3T1J8 (Replication termination factor 2 OS=Rattus norvegicus OX=10116 GN=Rtf2 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 4.0e-34
Identity = 97/271 (35.79%), Postives = 145/271 (53.51%), Query Frame = 0

Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK+++++ LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEVLRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 172 --HVKGLKDMIKISLSAIPGTESGSNAISG--------PRFQCPITGLEFNGKYKFFALR 231
             H++ +K++ ++ LS  P  E       G         RF CP+ GLE NG+++F  LR
Sbjct: 79  TSHIRSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 232 TCGHVLSAKALKEVKSSSCLVCHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSK-EKR 291
            CG V S +ALKE+K+  C  C A F++ D +V+NG++E+V +L+ RMEE + ++K EK+
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEEDIIVLNGTKEDVEMLKRRMEERRLRAKLEKK 198

Query: 292 LKKVKNGEVGMNGDVSVDLA--LSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVS 351
            KK K  E      +S D A      +GK    +    EK S+   R    +G    KV 
Sbjct: 199 TKKPKTAESASKLGISQDSAGPSKAKAGKSEEADPDPREKKSSLAPRGTASNGSASGKVG 258

Query: 352 ANN-GAVKRFKAADMAPPNATKEVYASIFTS 367
               GA+KR  A      +   E Y SIFTS
Sbjct: 259 KPPCGALKRSIA-----DSEESETYKSIFTS 284

BLAST of MS002351 vs. ExPASy Swiss-Prot
Match: Q99K95 (Replication termination factor 2 OS=Mus musculus OX=10090 GN=Rtf2 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.2e-32
Identity = 96/272 (35.29%), Postives = 144/272 (52.94%), Query Frame = 0

Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK+++++ LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 172 --HVKGLKDMIKISLSAIPGTESGSNAISG--------PRFQCPITGLEFNGKYKFFALR 231
             H++ +K++ ++ LS  P  E       G         RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIRSIKNVTELRLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 232 TCGHVLSAKALKEVKSSSCLVCHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSK-EKR 291
            CG V S +ALKE+K+  C  C A F++ D +V+NG++E+V +L++RMEE + ++K EK+
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEEDIIVLNGTKEDVEMLKKRMEERRLRAKLEKK 198

Query: 292 LKKVKNGE--VGMNGDVSVDLALSRL-SGKKHGIEVKASEKVSAKLERNERPDGGIQVKV 351
            KK K         G        S++ SGK    +    EK S    R    +G    KV
Sbjct: 199 TKKPKTATECASKPGTTQDSAGPSKVKSGKPEEADPDPREKKSTPAPRGAATNGSASGKV 258

Query: 352 SANN-GAVKRFKAADMAPPNATKEVYASIFTS 367
                GA+KR  A      +   E Y SIFTS
Sbjct: 259 GKPPCGALKRSIA-----DSEESETYKSIFTS 285

BLAST of MS002351 vs. ExPASy Swiss-Prot
Match: A5GFW7 (Replication termination factor 2 OS=Sus scrofa OX=9823 GN=RTF2 PE=3 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 3.8e-32
Identity = 94/281 (33.45%), Postives = 148/281 (52.67%), Query Frame = 0

Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK+++++ LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSSEKALGKA 78

Query: 172 --HVKGLKDMIKISLSAIPGTESGSNAISG--------PRFQCPITGLEFNGKYKFFALR 231
             H+K +K++ ++ LS  P  E    +  G         RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIKSIKNVTELRLSDNPAWEGDKGSTKGDKHDDLQRARFICPVVGLEMNGRHRFCYLR 138

Query: 232 TCGHVLSAKALKEVKSSSCLVCHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSK-EKR 291
            CG V S +ALKE+K+  C  C A F++ D +V+NG++E+VA+L+ RMEE + ++K  K+
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEDDVIVLNGTKEDVAMLQTRMEERRLRAKLGKK 198

Query: 292 LKKVKNGEVGMNGDVSVDL--ALSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVS 351
            KK K  E     D+S +        +GK     + + EK ++   R+    G    K  
Sbjct: 199 TKKPKAAESVSKSDISEEAPGPSKMKAGKPEETSLDSREKKTSSAPRSAAAHGSSSGKAG 258

Query: 352 ANNGAVKRFKAADMAPPNATKEVYASIFT--SSRKSDFKET 376
                  +   AD    +   E Y S+FT  SS K   +E+
Sbjct: 259 KPVCGAPKRSIAD----SGESEAYKSLFTTHSSAKRSKEES 295

BLAST of MS002351 vs. ExPASy Swiss-Prot
Match: Q5R9P9 (Replication termination factor 2 OS=Pongo abelii OX=9601 GN=RTF2 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.9e-31
Identity = 98/288 (34.03%), Postives = 148/288 (51.39%), Query Frame = 0

Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK+++++ LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 172 --HVKGLKDMIKISLSAIPGTESGSNAISG--------PRFQCPITGLEFNGKYKFFALR 231
             H+K +K++ ++ LS  P  E       G         RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 232 TCGHVLSAKALKEVKSSSCLVCHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSK-EKR 291
            CG V S +ALKE+K+  C  C A F++ D +V+NG++E+V VL+ RMEE + ++K EK+
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEDDVIVLNGTKEDVDVLKTRMEERRLRAKLEKK 198

Query: 292 LKKVKNGEVGMNGDVSVDLALSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVSAN 351
            KK K  E     DVS +             +VK  +   A L+  E+         + N
Sbjct: 199 TKKPKAAESVSKPDVSEE--------APGPSKVKTGKPEEASLDSREKKTNLAPKSTATN 258

Query: 352 NGAVKRFKAADMAPPNATK---------EVYASIFT--SSRKSDFKET 376
             +  +   A   P  ATK         E Y S+FT  SS K   +E+
Sbjct: 259 ESSSGK---AGKPPCGATKRSIADSEESEAYKSLFTTHSSAKRSKEES 295

BLAST of MS002351 vs. ExPASy Swiss-Prot
Match: Q9BY42 (Replication termination factor 2 OS=Homo sapiens OX=9606 GN=RTF2 PE=1 SV=3)

HSP 1 Score: 136.7 bits (343), Expect = 5.4e-31
Identity = 97/288 (33.68%), Postives = 148/288 (51.39%), Query Frame = 0

Query: 112 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFG-- 171
           KK +KVD + + +++W  C LS E LR P V   LG ++NK+++++ LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 172 --HVKGLKDMIKISLSAIPGTESGSNAISG--------PRFQCPITGLEFNGKYKFFALR 231
             H+K +K++ ++ LS  P  E       G         RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 232 TCGHVLSAKALKEVKSSSCLVCHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSK-EKR 291
            CG V S +ALKE+K+  C  C A F++ D +++NG++E+V VL+ RMEE + ++K EK+
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEDDVIMLNGTKEDVDVLKTRMEERRLRAKLEKK 198

Query: 292 LKKVKNGEVGMNGDVSVDLALSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVSAN 351
            KK K  E     DVS +             +VK  +   A L+  E+         + N
Sbjct: 199 TKKPKAAESVSKPDVSEE--------APGPSKVKTGKPEEASLDSREKKTNLAPKSTAMN 258

Query: 352 NGAVKRFKAADMAPPNATK---------EVYASIFT--SSRKSDFKET 376
             +  +   A   P  ATK         E Y S+FT  SS K   +E+
Sbjct: 259 ESSSGK---AGKPPCGATKRSIADSEESEAYKSLFTTHSSAKRSKEES 295

BLAST of MS002351 vs. ExPASy TrEMBL
Match: A0A6J1C136 (Replication termination factor 2 OS=Momordica charantia OX=3673 GN=LOC111007406 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 9.5e-212
Identity = 381/386 (98.70%), Postives = 384/386 (99.48%), Query Frame = 0

Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILDLAQTSAQTLEDLKFSLLPQTLAPRFASSFYFTLN 60
           MHPKNQQRLQIFVHSPDLQIQSKIL+LA+TSAQTLEDLKFSLLPQTLAPR ASSFYFTLN
Sbjct: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLALS 300
           CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLA S
Sbjct: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVSANNGAVKRFKAADMAPPNATKEVY 360
           RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKV+ANNGAVKRFKAADMAPPNATKEVY
Sbjct: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY 360

Query: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 387
           ASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of MS002351 vs. ExPASy TrEMBL
Match: A0A5A7SWS4 (Replication termination factor 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00470 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.1e-183
Identity = 337/388 (86.86%), Postives = 355/388 (91.49%), Query Frame = 0

Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILDLAQTSAQTLEDLKFSLLPQTLAPRFASSFYFTLN 60
           MHPKNQ RLQIF+HSPDLQIQSKI+DL QTSA+TLEDLKFSLL +TLA R ASS YFTLN
Sbjct: 1   MHPKNQHRLQIFLHSPDLQIQSKIVDLPQTSAKTLEDLKFSLLSETLASRVASSLYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDST  T SLI PLSTLILRTR+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDST--TISLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPL EPCVIDWLGNVFNKESLVQALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLSEPCVIDWLGNVFNKESLVQALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           + S IPGTES  NAIS PRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NFSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEE--KSKSKEKRLKKVKNGEVGMNGDVSVDLA 300
           CH EF +RDK VINGSEEEV  +RERMEEE  KSKSKEK+ KKV+NGEVGMNGDV++DLA
Sbjct: 241 CHTEFTERDKFVINGSEEEVEEMRERMEEEKSKSKSKEKKTKKVRNGEVGMNGDVTMDLA 300

Query: 301 LSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVSANNGAVKRFKAADMAPPNATKE 360
            SRLSGKKHGIE++  EKVSAK ER+ER DGG QVKV+A+NGAVKRFKAADM P NATKE
Sbjct: 301 TSRLSGKKHGIELQTLEKVSAKPERHERLDGGAQVKVAASNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 387
           VYASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of MS002351 vs. ExPASy TrEMBL
Match: A0A1S3B9F3 (Replication termination factor 2 OS=Cucumis melo OX=3656 GN=LOC103487489 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.1e-183
Identity = 337/388 (86.86%), Postives = 355/388 (91.49%), Query Frame = 0

Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILDLAQTSAQTLEDLKFSLLPQTLAPRFASSFYFTLN 60
           MHPKNQ RLQIF+HSPDLQIQSKI+DL QTSA+TLEDLKFSLL +TLA R ASS YFTLN
Sbjct: 1   MHPKNQHRLQIFLHSPDLQIQSKIVDLPQTSAKTLEDLKFSLLSETLASRVASSLYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDST  T SLI PLSTLILRTR+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDST--TISLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPL EPCVIDWLGNVFNKESLVQALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLSEPCVIDWLGNVFNKESLVQALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           + S IPGTES  NAIS PRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NFSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEE--KSKSKEKRLKKVKNGEVGMNGDVSVDLA 300
           CH EF +RDK VINGSEEEV  +RERMEEE  KSKSKEK+ KKV+NGEVGMNGDV++DLA
Sbjct: 241 CHTEFTERDKFVINGSEEEVEEMRERMEEEKSKSKSKEKKTKKVRNGEVGMNGDVTMDLA 300

Query: 301 LSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVSANNGAVKRFKAADMAPPNATKE 360
            SRLSGKKHGIE++  EKVSAK ER+ER DGG QVKV+A+NGAVKRFKAADM P NATKE
Sbjct: 301 TSRLSGKKHGIELQTLEKVSAKPERHERLDGGAQVKVAASNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 387
           VYASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of MS002351 vs. ExPASy TrEMBL
Match: A0A6J1F067 (Replication termination factor 2 OS=Cucurbita moschata OX=3662 GN=LOC111440971 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 7.1e-183
Identity = 330/386 (85.49%), Postives = 352/386 (91.19%), Query Frame = 0

Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILDLAQTSAQTLEDLKFSLLPQTLAPRFASSFYFTLN 60
           M+PKNQ RLQ FVHSPDLQIQSKI++LAQTSAQ+L DLK SL+  T A R ASSFYFTLN
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDS  ++SSL+ PLSTLILRTR+ GGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGN+FNKE LV ALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           +LS +PGTES  NAIS  RFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NLSMVPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLALS 300
           CHAEF DRDK VINGSEEEV  +RERMEEEK+KSKEK+ KKV+ GEVGMNGDVSVDLA S
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVSANNGAVKRFKAADMAPPNATKEVY 360
           RLSGKKHGIE KA  KVSAK E++ERPD G+QVKV+A+NGAVKRFKAADMAP NATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKPEKHERPDSGVQVKVAASNGAVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 387
           ASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of MS002351 vs. ExPASy TrEMBL
Match: A0A0A0LTI5 (Replication termination factor 2 OS=Cucumis sativus OX=3659 GN=Csa_1G269330 PE=3 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.7e-181
Identity = 333/388 (85.82%), Postives = 352/388 (90.72%), Query Frame = 0

Query: 1   MHPKNQQRLQIFVHSPDLQIQSKILDLAQTSAQTLEDLKFSLLPQTLAPRFASSFYFTLN 60
           MHPKNQ R QIF+ SPDLQI+SKI++L QT A+TLEDLKFSLL + LA R ASSFYFTLN
Sbjct: 1   MHPKNQHRFQIFLQSPDLQIESKIVNLPQTPAKTLEDLKFSLLTEILASRIASSFYFTLN 60

Query: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPLLDST  T SLI PLSTLILRTR+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDST--TISLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGH+KGLKDMIKI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240
           + S IPGTES  NAIS PR+QCP+TGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV
Sbjct: 181 NFSMIPGTESRGNAISEPRYQCPVTGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEE--KSKSKEKRLKKVKNGEVGMNGDVSVDLA 300
           CHAEF +RDK VINGSEEEV  +RERMEEE  KSKSKEK+ KKV+NGEVGMNGDVSVDLA
Sbjct: 241 CHAEFAERDKFVINGSEEEVEEMRERMEEEKSKSKSKEKKTKKVRNGEVGMNGDVSVDLA 300

Query: 301 LSRLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVSANNGAVKRFKAADMAPPNATKE 360
           +SRLSGKKH IEVK+ EKVSAK ER ER DGG QVK + +NGAVKRFKAADM P NATKE
Sbjct: 301 ISRLSGKKHAIEVKSLEKVSAKPERQERLDGGAQVKDATSNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 387
           VYASIFTSSRKSDFKETYSCRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of MS002351 vs. TAIR 10
Match: AT5G58020.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF602 (InterPro:IPR006735); Has 385 Blast hits to 385 proteins in 189 species: Archae - 0; Bacteria - 0; Metazoa - 153; Fungi - 117; Plants - 50; Viruses - 0; Other Eukaryotes - 65 (source: NCBI BLink). )

HSP 1 Score: 397.1 bits (1019), Expect = 1.6e-110
Identity = 229/384 (59.64%), Postives = 268/384 (69.79%), Query Frame = 0

Query: 8   RLQIFVHSPDLQIQSKILDLAQTSAQTLEDLK--FSLLPQTLAPRFASSFYFTLNGKPLL 67
           R QIFV SPD Q   K++ L    AQ+L  L    SLL  +    F S+   TL+GK L 
Sbjct: 4   RRQIFVKSPDCQ---KVVALQLDPAQSLLTLSGITSLLESSQRISF-SACSITLDGKLLN 63

Query: 68  DSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLS 127
            ST +  S +  +S L L  R+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLS
Sbjct: 64  GSTRIQVSKLPSVSMLTLFPRLRGGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLS 123

Query: 128 KWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKISLSAI 187
           KWLNCALSNEPL EPCVID LGN+FNKE LV ALL K+LPK F ++KGLKDM+ I L+ +
Sbjct: 124 KWLNCALSNEPLAEPCVIDLLGNLFNKEVLVHALLSKRLPKQFSYIKGLKDMVNIKLTPV 183

Query: 188 PGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLVCHAEF 247
            G++  S   +  +FQCP++GLEFNGKYKFFALR CGHV+SAKALKEVKSSSCLVCHA+ 
Sbjct: 184 AGSDGSSQDTTSAQFQCPVSGLEFNGKYKFFALRGCGHVMSAKALKEVKSSSCLVCHADV 243

Query: 248 KDRDKLVINGSEEEVAVLRERMEEEKSKSKEKR--LKKVKNGEVGMNGDVSVDLALSRLS 307
           KD DK+VING+EEEV +LRERMEEEK+K +EK+   KK KNG       V  D       
Sbjct: 244 KDSDKIVINGTEEEVDLLRERMEEEKAKLREKKGVSKKSKNGAA-----VVAD------- 303

Query: 308 GKKHGIEVKASEKVSAKLERNERPDGGIQVKVSANNGAVKRFKAADMAPPNATKEVYASI 367
                          AK+ + +  DG     V+ N   VK+FKAAD  P NATKEVYAS+
Sbjct: 304 -------------TGAKVAKRQIEDG----NVNGNGITVKKFKAADKVPVNATKEVYASL 354

Query: 368 FTSS-RKSDFKETYSCRSLPLGRN 387
           FTSS +KSDF+ETYSCRSLPLGRN
Sbjct: 364 FTSSKKKSDFRETYSCRSLPLGRN 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135450.12.0e-21198.70protein RTF2 homolog [Momordica charantia][more]
XP_038879920.12.7e-18486.34replication termination factor 2 [Benincasa hispida][more]
XP_008444038.12.2e-18386.86PREDICTED: protein RTF2 homolog [Cucumis melo] >KAA0035058.1 protein RTF2-like p... [more]
XP_022933589.11.5e-18285.49protein RTF2 homolog [Cucurbita moschata][more]
KAG7035670.11.9e-18285.75Protein RTF2-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q3T1J84.0e-3435.79Replication termination factor 2 OS=Rattus norvegicus OX=10116 GN=Rtf2 PE=2 SV=1[more]
Q99K952.2e-3235.29Replication termination factor 2 OS=Mus musculus OX=10090 GN=Rtf2 PE=1 SV=1[more]
A5GFW73.8e-3233.45Replication termination factor 2 OS=Sus scrofa OX=9823 GN=RTF2 PE=3 SV=1[more]
Q5R9P91.9e-3134.03Replication termination factor 2 OS=Pongo abelii OX=9601 GN=RTF2 PE=2 SV=1[more]
Q9BY425.4e-3133.68Replication termination factor 2 OS=Homo sapiens OX=9606 GN=RTF2 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1C1369.5e-21298.70Replication termination factor 2 OS=Momordica charantia OX=3673 GN=LOC111007406 ... [more]
A0A5A7SWS41.1e-18386.86Replication termination factor 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3B9F31.1e-18386.86Replication termination factor 2 OS=Cucumis melo OX=3656 GN=LOC103487489 PE=3 SV... [more]
A0A6J1F0677.1e-18385.49Replication termination factor 2 OS=Cucurbita moschata OX=3662 GN=LOC111440971 P... [more]
A0A0A0LTI51.7e-18185.82Replication termination factor 2 OS=Cucumis sativus OX=3659 GN=Csa_1G269330 PE=3... [more]
Match NameE-valueIdentityDescription
AT5G58020.11.6e-11059.64unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF602 ... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 253..280
NoneNo IPR availablePFAMPF04641Rtf2coord: 90..370
e-value: 6.8E-72
score: 242.0
NoneNo IPR availablePANTHERPTHR12775:SF1BNACNNG39770D PROTEINcoord: 7..298
NoneNo IPR availablePANTHERPTHR12775:SF1BNACNNG39770D PROTEINcoord: 332..386
IPR006735Replication termination factor 2PANTHERPTHR12775PROTEIN C20ORF43 HOMOLOGcoord: 332..386
IPR006735Replication termination factor 2PANTHERPTHR12775PROTEIN C20ORF43 HOMOLOGcoord: 7..298
IPR000626Ubiquitin-like domainPROSITEPS50053UBIQUITIN_2coord: 9..90
score: 8.633156
IPR027799Replication termination factor 2, RING-fingerCDDcd16653RING-like_Rtf2coord: 200..245
e-value: 8.04194E-27
score: 99.2375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002351.1MS002351.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902979 mitotic DNA replication termination
molecular_function GO:0005515 protein binding