MS002336 (gene) Bitter gourd (TR) v1

Overview
NameMS002336
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionWRKY protein
Locationscaffold30: 4199923 .. 4204696 (-)
RNA-Seq ExpressionMS002336
SyntenyMS002336
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGAGCGCGAAGGCTGTGAAACTGACCAACTCGGAAGCTCCAAGGCTACTGGAGGGCAGGAAGACGATGAGGAAGAAGAGATGGAAGATTCGGAGGATGAATCAGAGGTTGAGTTGGAAGATGGAGAAGGACGGGTCAGTGAGTCGCAACCGGCTGAGTTGAGGGCGGTTTCGTCGGTTAATGGAACTGCGGCGAGTGGGTCTCGTCCTGAAACCCTAGCGGCGCCTTCTGCGATTCAGTCATCGGAGAACGGTCGGTCTGATGGTAGGTTTGTTTGAGAGAGATTTGATTCTGTCTCGGTGAATGAGTTATTCGGTTTCTGAATCATTTCTAAGTTTTGGTGGAATTTTTCTGTGGGTGATGTTCTGCTGTTGATTCTACAGGTTTTCTTGTCAATTCTGCTACGCAGTCGCTCCAAGGAGCTGAATTGAAGGTAAGGAGCTCGATCATTTCTGTGAGGACCCAATTTTTTTTCTTTCATGTCTTTATTTTCTCTCTCTACTCGCTAAACTCATTAACAATTGTTTGCTCGTGTGGGTTTTATTGGTTTGATATTGTATTCGCTATTTCAATAGTGAAAGTTAAACTGGAAAATTGAAGTAGTTTTAAAGTAATTATCATTGGTTTTAATTCCCGAATCACCCTTCAGTTGTATCTTTGTCAAAAGAAAAAGGGTTTGCGCAGCACGGGAACATTGTTGTTTACGAGGAATATTTTTTGCTAATTATTCGGGGTTTGAGAGTAATGAGAGGCAGGTAACTTCCGTTTCTCCTTGTATACCCTCCTGTTGAAGTTGAACCGATCTTTCTCAAATAAAGCTCCAGCCAGTCAGCTACTTATCTCAATCTGCAAATTACTCAAGTACTACTGAGCTTCTGTCTCTGTGCCTGGCTAATTCTTTGTGCTTGAAAAGGGAAAACTTGAATAATATGAAACGTTGGTTTTCTTGCGATCATATTTTTTGTGATCCTGTGTTAAAGCCAACCACGAGCATGATACGCAAAACATCATGTCAGTTTTTACATTTACTGAGCTAGAACATCTTCTTTCAGATCTTATTGGAAGGAACAAAGTCTGCAAGTATTTTAAATTCTCATTTGAAATGTATCTCCTTCATATATTAGTCTCGGTTGTGCTTGTTGGTGTCTTTCCTTAGTTCAATGGCATGATTCTGCAGAAATATGACCTAAATATTTGTTGCGAGCTGATTTTTTTCTAGGCACAGTGGCATGATTATGCAGGTATATGCTGCCAATGAAATTTCCATGAATAAAACAGAATGTGATAATTGGTTTTATTTTTTAAACAAAAAAAGAAAAACATTGGATCTACTATCTTACTTTTAATCCTTTGCTTTTGATTCACTGAGAGAGGAATTACTCCCGTTCCTAATTCAGATGTTAAAGCTTCATTTTGTCAATTGTGAATCTTGTATGAATGAGCAGCCTAGTTCAGAGTAAATGCCATATAAAGCATGCATGCGAATACTTTGTAGCACTATATTCATATCTAATGCAAAATGATATACATTTTCACTGGAATTCCATAAAGATGCAAGAGTTTTGCTTTTTCTTTAATGCATTTCTATGTAGGGAATTGCTTGAAATGGTATACGTAAAAGAACCAGGAAGCCTGAAAATTGAATAGGTGAACAAAATTAAAAGCAGAACAATAAATAGCATTTTGATGTGCATGGAGATTAACATTATTCTCTTCATCCAAGTTCTATGCAATCAAAGTACTGCCAAGAATGCGCAGTTTCCTCCAAGGAGGGAAGAGGTGCTTCCCTAGGTTTTGGTCTCTGATTATAATTCAAAAGTGGGTATCTATTCTTTTGAAGGCTTAAGCTCTTCAATGCAGTAGCAATATTTAGAAAGTGTCCTTTATGCTTGTGCCTGTAGGATATTTAGTCAATATGAGAATACCTTCCTTAAAGACATTCTTTCTGTTTTCTCATTATTACATTAATGTGGGTGCATTACAAGTTAGTTCTGTCATCAAGTATGTTGCATCGCATTTCATCACAATAGCATTATATGATGTGGGTGTCCTGTAATTGAGAAGTTTGAGATTTGAAGGCGAATGATTGGGACATAGATCTTTTGGCCTTACTTATTTTAGCCTTGTACAGTTTCTCTCGCTTAACTGTGTACCATTTTGCTTCTTTTGTGGGTCATCTCCTCGAAAATGTAATTACTCCTACTTCACAAGTTAGCTAATCTAGAATCCCAGCCAAAAAAATGAAAAATATACGGTAGGTGGCTATTAGGAAAATAAATACTTCAGATAGAATGTAATAGATATTTACTCTCTGGCTGACAACCTGCAGATATGTGTGCTTTGTGAGAGTGCTGCTGGTACTATTGAATACGTTTCTTCCAATAGTCATTTTTGTGTGGGGTTTGTTTTTGAACTTTTTTTGTTGCTTTTGTGTCCTCCGGGTAGCCTTTAGGAAAGAGAGTTGGGGTTTCTTTACAAGTCTTATAAGAAGTAAGTGTATGATTGAAGAGACTGCAGTTTCCACTCAACTAATTATCATATTAATACTGATCTTTTGTGATCGAACATGAAGCATGTATGAATCCTTGGTCATATTTTTTATGTTTTCCCCATATTATTTAAAACTATGGTTCATGTCCTGTTGTTTAAATACTTTCACTTTCTGGTTTTGAAGTTCATATTTTCCTTCATTACTAGTAGATTGTAGTTTCCCATCAACATATGTTCTTGTTGCAAAGCTTACTCACTGGGTATTTATCCCCCTCAAAGATTCAGTTTAAGTTGTTCATTTGAGGGGTAAGTGATTAGTCATTGATGATGTTTTCTCTATGAGCCTGGAAGATTTGTGATAACTTGAAATGTGAAAATTTCAAATGAACAATCTGATGTAACTTAAATTTCTTCGAGACACATATTTTGAATTAATAAGTTCCTTACGATGAAGTCAGGAAAGTTTGTAGAACGGCTGAGAGCTAGACTCATTGTTGATGATTTGATGCCTTAGTATATTGCTGTCCTCACTGTCTAATTTATTTTTCTCATTCTCCTATAATCAGCCGGTTCTATCTTCCTGCAATGAGCCTTTGGCTGTTGAAGCAGTCCAGACAGATCAGGTGCAAGAACAAGGTGCAACTTGTAAAGGACCTGGTTCGGATCAGTCGCCAACTTCAGTAACCCAGTCTATCTCATCCTCTGCAAGCCCAAGTTTATCTGAACATAAACTGTCACCCAAGAAGGTCCATAAAGAATGTCAGCCAGAACCGAGCCAGAAAAATTCTTCCGACCGTAGAACTGTATCTCCTGTTGTTAATGTTAGGACACCTGCTTCTGATGGTTACAATTGGAGGAAATATGGTCAGAAGCAAGTGAAGAGTCCCAAGGGTTCACGTAGCTATTACAAGTGTACATATTCTGAATGCTGTGCTAAGAAGATTGAATGTTGTGATCACTCAGGCCACGTAACAGAGATTGTTTACAAGAGCCAACATAGCCATGATCCACCCAGGAAGATTAACAACCCAAAGGAAAGCAAGCTTGTACCATATGTTGAACCTGTGGTTAAAAAAATCATTGCAGAACATTCCAGAAGAATAATTAGTGATTCAGATCCTCCCACGCCTTCAAAAGAACCTTTACGGGAAACAGCTATAGTCCTCGAAAGAAAACGACAGTACTCAAACGACTCTGATGGAAATGAAGAATTTAAGGTCAAAGATGAGAATGTCAATGAACCTGAGACAAAACTAAAGTAAGTCGGCACCTCCAGTTGCAGAGCCCTTGTTCTTATTTCTACTCCTGTATAAAATGCTTAGAAAGTATTAGCTTTGACATAGTGCTAACCTTCTATTTCCTTACTGGTTATTAGAGTTAAGAAAAGCAGTGCCGGGTACTCAGGTTCTCCCTTAAAACCTGGAAAGAAACCAAAATTTGTGGTGCATGCTGCAGGCGATGTGGGAATCTCGGGTGATGGATATAGATGGCGCAAGTATGGTCAAAAAATGGTGAAAGGAAATCCTCATCCTAGGTGAATACCTTTTTATAATTTATGTCACACTGCTTATTTGATGGAAAACAAGACAGTTTTTTAATTAGACTAGTTTTGTTTATTCCCGATTGTCCTGGACCATTCTGCTGGTGCTGAAATATATGTGTTTTTGTTATATTACATGCTTATTGTCAGATTTTGCTTTGGTTCCATGGAATTGTCTCCAGTTCTGTTATTCCATTGCGTCTATCAATTGAACTTAATCTTATTGCAGTCATCATGTGAAATTACCGCTCATACTCCATAATTTGGAAAACTATCTTCATGTTAAGAACAGAAAGAGAACAAACATCTCCAATTAATTTTGACTAAATCATTCTCTGTTCCTTTGCATCGTCTGATCTCGATGTTGGTGGAATAAAATTTTCAGGAACTACTACAGATGTACCTCTGCTGGGTGTCCCGTCCGCAAGCACATTGAATCAGCTGTGGAAAATCCAAACGCAGTGATTATAACATACAAGGGAATTCATGACCATGACACGCCCGTTCCAAAGAAACGACACGGTCCACCAAGCGCTCCTCTTGTAGCTGCTGCAGCTCCAGCCTCCATGAACAATATGCAACCGAAAAAAACAGACGCTGTTCAGAGCCAAATTTCTTCGACACAGTGGTCTGTGGATGCTGAAGGAGAGCTGACTGGTGAGGCCTTGGACCTTGGAGGTGAGAAAGCCATGGAATCTGCTCGAACTCTTCTAAGCATTGGATTTGAAATCAAGCCCTGC

mRNA sequence

ATGGCTGAGCGCGAAGGCTGTGAAACTGACCAACTCGGAAGCTCCAAGGCTACTGGAGGGCAGGAAGACGATGAGGAAGAAGAGATGGAAGATTCGGAGGATGAATCAGAGGTTGAGTTGGAAGATGGAGAAGGACGGGTCAGTGAGTCGCAACCGGCTGAGTTGAGGGCGGTTTCGTCGGTTAATGGAACTGCGGCGAGTGGGTCTCGTCCTGAAACCCTAGCGGCGCCTTCTGCGATTCAGTCATCGGAGAACGGTCGGTCTGATGGTAGTATATTGCTGTCCTCACTGTCTAATTTATTTTTCTCATTCTCCTATAATCAGCCGGTTCTATCTTCCTGCAATGAGCCTTTGGCTGTTGAAGCAGTCCAGACAGATCAGGTGCAAGAACAAGGTGCAACTTGTAAAGGACCTGGTTCGGATCAGTCGCCAACTTCAGTAACCCAGTCTATCTCATCCTCTGCAAGCCCAAGTTTATCTGAACATAAACTGTCACCCAAGAAGGTCCATAAAGAATGTCAGCCAGAACCGAGCCAGAAAAATTCTTCCGACCGTAGAACTGTATCTCCTGTTGTTAATGTTAGGACACCTGCTTCTGATGGTTACAATTGGAGGAAATATGGTCAGAAGCAAGTGAAGAGTCCCAAGGGTTCACGTAGCTATTACAAGTGTACATATTCTGAATGCTGTGCTAAGAAGATTGAATGTTGTGATCACTCAGGCCACGTAACAGAGATTGTTTACAAGAGCCAACATAGCCATGATCCACCCAGGAAGATTAACAACCCAAAGGAAAGCAAGCTTGTACCATATGTTGAACCTGTGGTTAAAAAAATCATTGCAGAACATTCCAGAAGAATAATTAGTGATTCAGATCCTCCCACGCCTTCAAAAGAACCTTTACGGGAAACAGCTATAGTCCTCGAAAGAAAACGACAGTACTCAAACGACTCTGATGGAAATGAAGAATTTAAGGTCAAAGATGAGAATGTCAATGAACCTGAGACAAAACTAAAAGTTAAGAAAAGCAGTGCCGGGTACTCAGGTTCTCCCTTAAAACCTGGAAAGAAACCAAAATTTGTGGTGCATGCTGCAGGCGATGTGGGAATCTCGGGTGATGGATATAGATGGCGCAAGTATGGTCAAAAAATGGTGAAAGGAAATCCTCATCCTAGGAACTACTACAGATGTACCTCTGCTGGGTGTCCCGTCCGCAAGCACATTGAATCAGCTGTGGAAAATCCAAACGCAGTGATTATAACATACAAGGGAATTCATGACCATGACACGCCCGTTCCAAAGAAACGACACGGTCCACCAAGCGCTCCTCTTGTAGCTGCTGCAGCTCCAGCCTCCATGAACAATATGCAACCGAAAAAAACAGACGCTGTTCAGAGCCAAATTTCTTCGACACAGTGGTCTGTGGATGCTGAAGGAGAGCTGACTGGTGAGGCCTTGGACCTTGGAGGTGAGAAAGCCATGGAATCTGCTCGAACTCTTCTAAGCATTGGATTTGAAATCAAGCCCTGC

Coding sequence (CDS)

ATGGCTGAGCGCGAAGGCTGTGAAACTGACCAACTCGGAAGCTCCAAGGCTACTGGAGGGCAGGAAGACGATGAGGAAGAAGAGATGGAAGATTCGGAGGATGAATCAGAGGTTGAGTTGGAAGATGGAGAAGGACGGGTCAGTGAGTCGCAACCGGCTGAGTTGAGGGCGGTTTCGTCGGTTAATGGAACTGCGGCGAGTGGGTCTCGTCCTGAAACCCTAGCGGCGCCTTCTGCGATTCAGTCATCGGAGAACGGTCGGTCTGATGGTAGTATATTGCTGTCCTCACTGTCTAATTTATTTTTCTCATTCTCCTATAATCAGCCGGTTCTATCTTCCTGCAATGAGCCTTTGGCTGTTGAAGCAGTCCAGACAGATCAGGTGCAAGAACAAGGTGCAACTTGTAAAGGACCTGGTTCGGATCAGTCGCCAACTTCAGTAACCCAGTCTATCTCATCCTCTGCAAGCCCAAGTTTATCTGAACATAAACTGTCACCCAAGAAGGTCCATAAAGAATGTCAGCCAGAACCGAGCCAGAAAAATTCTTCCGACCGTAGAACTGTATCTCCTGTTGTTAATGTTAGGACACCTGCTTCTGATGGTTACAATTGGAGGAAATATGGTCAGAAGCAAGTGAAGAGTCCCAAGGGTTCACGTAGCTATTACAAGTGTACATATTCTGAATGCTGTGCTAAGAAGATTGAATGTTGTGATCACTCAGGCCACGTAACAGAGATTGTTTACAAGAGCCAACATAGCCATGATCCACCCAGGAAGATTAACAACCCAAAGGAAAGCAAGCTTGTACCATATGTTGAACCTGTGGTTAAAAAAATCATTGCAGAACATTCCAGAAGAATAATTAGTGATTCAGATCCTCCCACGCCTTCAAAAGAACCTTTACGGGAAACAGCTATAGTCCTCGAAAGAAAACGACAGTACTCAAACGACTCTGATGGAAATGAAGAATTTAAGGTCAAAGATGAGAATGTCAATGAACCTGAGACAAAACTAAAAGTTAAGAAAAGCAGTGCCGGGTACTCAGGTTCTCCCTTAAAACCTGGAAAGAAACCAAAATTTGTGGTGCATGCTGCAGGCGATGTGGGAATCTCGGGTGATGGATATAGATGGCGCAAGTATGGTCAAAAAATGGTGAAAGGAAATCCTCATCCTAGGAACTACTACAGATGTACCTCTGCTGGGTGTCCCGTCCGCAAGCACATTGAATCAGCTGTGGAAAATCCAAACGCAGTGATTATAACATACAAGGGAATTCATGACCATGACACGCCCGTTCCAAAGAAACGACACGGTCCACCAAGCGCTCCTCTTGTAGCTGCTGCAGCTCCAGCCTCCATGAACAATATGCAACCGAAAAAAACAGACGCTGTTCAGAGCCAAATTTCTTCGACACAGTGGTCTGTGGATGCTGAAGGAGAGCTGACTGGTGAGGCCTTGGACCTTGGAGGTGAGAAAGCCATGGAATCTGCTCGAACTCTTCTAAGCATTGGATTTGAAATCAAGCCCTGC

Protein sequence

MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQPAELRAVSSVNGTAASGSRPETLAAPSAIQSSENGRSDGSILLSSLSNLFFSFSYNQPVLSSCNEPLAVEAVQTDQVQEQGATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPEPSQKNSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHSGHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTPSKEPLRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPGKKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVIITYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSVDAEGELTGEALDLGGEKAMESARTLLSIGFEIKPC
Homology
BLAST of MS002336 vs. NCBI nr
Match: XP_022135102.1 (probable WRKY transcription factor 32 [Momordica charantia])

HSP 1 Score: 944.1 bits (2439), Expect = 4.9e-271
Identity = 493/510 (96.67%), Postives = 499/510 (97.84%), Query Frame = 0

Query: 1   MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQPAELRAVSS 60
           MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQ AELRAVSS
Sbjct: 1   MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQSAELRAVSS 60

Query: 61  VNGTAASGSRPETLAAPSAIQSSENGRSDGSILLSSLSNLFFSFSYNQPVLSSCNEPLAV 120
           VNGTAASGSRPETLAAPSAIQSSENGRSDG ++ S+  +L    +  +PVLSSCNEPLAV
Sbjct: 61  VNGTAASGSRPETLAAPSAIQSSENGRSDGFLVNSATQSL--QGAELKPVLSSCNEPLAV 120

Query: 121 EAVQTDQVQEQGATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPEPSQK 180
           EAVQTDQVQEQGATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPEPSQK
Sbjct: 121 EAVQTDQVQEQGATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPEPSQK 180

Query: 181 NSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHS 240
           NSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHS
Sbjct: 181 NSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHS 240

Query: 241 GHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTPSKEP 300
           GHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTPSKEP
Sbjct: 241 GHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTPSKEP 300

Query: 301 LRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPGKKPKF 360
           LRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPGKKPKF
Sbjct: 301 LRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPGKKPKF 360

Query: 361 VVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVII 420
           VVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVII
Sbjct: 361 VVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVII 420

Query: 421 TYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSVDAEGE 480
           TYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSVDAEGE
Sbjct: 421 TYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSVDAEGE 480

Query: 481 LTGEALDLGGEKAMESARTLLSIGFEIKPC 511
           LTGEALDLGGEKAMESARTLLSIGFEIKPC
Sbjct: 481 LTGEALDLGGEKAMESARTLLSIGFEIKPC 508

BLAST of MS002336 vs. NCBI nr
Match: XP_038879508.1 (probable WRKY transcription factor 32 [Benincasa hispida])

HSP 1 Score: 763.8 bits (1971), Expect = 9.2e-217
Identity = 419/517 (81.04%), Postives = 444/517 (85.88%), Query Frame = 0

Query: 1   MAEREGCETDQLGSSK-ATGGQEDDEEEEMEDSEDESEVELE--DGEGRVSESQPAELRA 60
           MAE E  ETDQLGSSK AT GQEDD EEEMEDSE+ESEVELE   G G VSE QP ELR 
Sbjct: 1   MAEPESFETDQLGSSKAATAGQEDD-EEEMEDSENESEVELERGGGGGVVSELQPTELRT 60

Query: 61  VSSVNGTAASGSRPETLAAPSAIQSSENGRSDGSILLSSLSNLFFSFSYNQPVLSSCNEP 120
            SSV+     GS  ETL   S  QSSENGR DG + ++S +             SS NEP
Sbjct: 61  GSSVSEAVVRGSCSETLTVLSMNQSSENGRCDG-LPVNSAAQSLEGAELKPQARSSRNEP 120

Query: 121 LAVEAVQTDQVQE----QGATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKEC 180
           +AVEA QTD+VQE    Q +TCKGP SDQSPTSVTQSISSSASPSLSEHK+SPKKV KEC
Sbjct: 121 VAVEATQTDKVQEESQLQVSTCKGPDSDQSPTSVTQSISSSASPSLSEHKMSPKKVQKEC 180

Query: 181 QPEPSQKNSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKK 240
           + EPSQKNSS+ +T S V NVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSEC AKK
Sbjct: 181 KAEPSQKNSSNHKTASSVPNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECFAKK 240

Query: 241 IECCDHSGHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDP 300
           IECCDHSG +T  VYKSQHSHDPPRKI+ PKES+LVPYVEPVVKKIIAEHSRR+I+DSDP
Sbjct: 241 IECCDHSGKITGTVYKSQHSHDPPRKISIPKESRLVPYVEPVVKKIIAEHSRRVINDSDP 300

Query: 301 PTPSKEPLRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLK 360
           P PSKEPL+ETAIVLERKRQYSNDSDGN+EFK+KDEN +EPETK KVKKSSAG SG+PLK
Sbjct: 301 PMPSKEPLQETAIVLERKRQYSNDSDGNDEFKIKDENDDEPETKQKVKKSSAGNSGTPLK 360

Query: 361 PGKKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVE 420
           PGKKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGN HPRNYYRCTSAGCPVRKHIESAVE
Sbjct: 361 PGKKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNAHPRNYYRCTSAGCPVRKHIESAVE 420

Query: 421 NPNAVIITYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQW 480
           NPNAVIITYKG+HDHD PVPKKRHGPPSAPLVAAAAP SM   QPKKTDAVQSQISSTQW
Sbjct: 421 NPNAVIITYKGVHDHDMPVPKKRHGPPSAPLVAAAAPVSMIYTQPKKTDAVQSQISSTQW 480

Query: 481 SVDAEGELTGEALDLGGEKAMESARTLLSIGFEIKPC 511
           SVDAEGELTGEAL+LGGEKAMESARTLLSIGFEIKPC
Sbjct: 481 SVDAEGELTGEALELGGEKAMESARTLLSIGFEIKPC 515

BLAST of MS002336 vs. NCBI nr
Match: XP_022988569.1 (probable WRKY transcription factor 32 [Cucurbita maxima])

HSP 1 Score: 759.6 bits (1960), Expect = 1.7e-215
Identity = 411/514 (79.96%), Postives = 444/514 (86.38%), Query Frame = 0

Query: 1   MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQPAELRAVSS 60
           MAER+  ETDQLGSSK T GQEDD EEEMEDSEDESEVELEDG G +SE QP ELR  SS
Sbjct: 1   MAERQCFETDQLGSSKVTAGQEDD-EEEMEDSEDESEVELEDG-GALSELQPIELRIDSS 60

Query: 61  VNGTAASGSRPETLAAPSAIQSSENGRSDGSILLSSLSNLFFSFSYNQPVLSSCNEPLAV 120
           V   AA  S  ETL APSA  SSENG      + S+  +L  +     P  SSC+EPLAV
Sbjct: 61  VTEAAARESPSETLTAPSANHSSENGLP----VDSAAQSLEEAELKRSP--SSCHEPLAV 120

Query: 121 EAVQTDQVQEQG----ATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPE 180
           EA QTD+VQEQ     +  KGP  +QSPTSVTQSISSSASPSLS+HKLSPKKV KEC+PE
Sbjct: 121 EATQTDKVQEQSQLQLSIYKGPDPEQSPTSVTQSISSSASPSLSKHKLSPKKVQKECKPE 180

Query: 181 PSQKNSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIEC 240
           PSQKNSS+ +T S V NVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIEC
Sbjct: 181 PSQKNSSNHKTASSVPNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIEC 240

Query: 241 CDHSGHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTP 300
           CDHSGH TEIVY+SQHSHDPPR I+NPKE +LVPYVEPVVK+II +H+RR+I+DSDPPTP
Sbjct: 241 CDHSGHRTEIVYRSQHSHDPPRMISNPKERQLVPYVEPVVKQIITQHTRRVINDSDPPTP 300

Query: 301 SKEPLRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPGK 360
           SKEP RETA+VL RKRQYS+DSDGN+EFK+KDEN  EPETK KVKKSSAGYSG+PLKPGK
Sbjct: 301 SKEPSRETALVLVRKRQYSSDSDGNDEFKIKDENDGEPETKQKVKKSSAGYSGTPLKPGK 360

Query: 361 KPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPN 420
           KPKFVVHAAGDVGISGDGYRWRKYGQKMVKG+PHPRNYYRCTSAGCPVRKHIESAVENP 
Sbjct: 361 KPKFVVHAAGDVGISGDGYRWRKYGQKMVKGSPHPRNYYRCTSAGCPVRKHIESAVENPY 420

Query: 421 AVIITYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSVD 480
           AVIITYKG+HDHD PVPKKRH PPSAP VAAAAPASM+N QPKKT+  +SQISSTQWSVD
Sbjct: 421 AVIITYKGVHDHDMPVPKKRHSPPSAPRVAAAAPASMSNTQPKKTNLTESQISSTQWSVD 480

Query: 481 AEGELTGEALDLGGEKAMESARTLLSIGFEIKPC 511
           AEGELTGEAL+LGGEKAMESARTLLSIGFEIKPC
Sbjct: 481 AEGELTGEALELGGEKAMESARTLLSIGFEIKPC 506

BLAST of MS002336 vs. NCBI nr
Match: KAG6589772.1 (putative WRKY transcription factor 32, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 755.0 bits (1948), Expect = 4.3e-214
Identity = 409/514 (79.57%), Postives = 438/514 (85.21%), Query Frame = 0

Query: 1   MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQPAELRAVSS 60
           MAER+  ETDQLGSSK T G EDD EEEMEDSEDESEVELEDG G +SE QP E R  SS
Sbjct: 1   MAERQCFETDQLGSSKVTAGLEDD-EEEMEDSEDESEVELEDG-GALSELQPVESRIDSS 60

Query: 61  VNGTAASGSRPETLAAPSAIQSSENGRSDGSILLSSLSNLFFSFSYNQPVLSSCNEPLAV 120
           V   AA  S  ETL APSA  SSENG     + + S +         Q   SSC+EPLAV
Sbjct: 61  VTEAAARESPSETLTAPSANHSSENG-----LPVDSAAQSLEEAELKQSP-SSCHEPLAV 120

Query: 121 EAVQTDQVQEQG----ATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPE 180
           EA QTD+VQEQ     +  KGP  +QSPTSVTQSISSSASPSLS+HKLSPKKV KEC+PE
Sbjct: 121 EATQTDKVQEQSQLQLSIYKGPDPEQSPTSVTQSISSSASPSLSKHKLSPKKVQKECKPE 180

Query: 181 PSQKNSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIEC 240
           PSQKNSS+ +T S V NVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIEC
Sbjct: 181 PSQKNSSNHKTASSVPNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIEC 240

Query: 241 CDHSGHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTP 300
           CDHSGH TEIVY+SQHSHDPPR I+NPKE +LVPYVEPVVK+II EHSRR+I+DSDPPTP
Sbjct: 241 CDHSGHRTEIVYRSQHSHDPPRMISNPKERQLVPYVEPVVKQIITEHSRRVINDSDPPTP 300

Query: 301 SKEPLRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPGK 360
           SKEP RETA VL RKRQYS+DSDGN+EFK+KDEN  EPETK KVKKSSAGYSG+PLKPGK
Sbjct: 301 SKEPSRETASVLVRKRQYSSDSDGNDEFKIKDENDGEPETKQKVKKSSAGYSGTPLKPGK 360

Query: 361 KPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPN 420
           KPKFVVHA GDVGISGDGYRWRKYGQKMVKG+PHPRNYYRCTSAGCPVRKHIESAVENP 
Sbjct: 361 KPKFVVHAVGDVGISGDGYRWRKYGQKMVKGSPHPRNYYRCTSAGCPVRKHIESAVENPY 420

Query: 421 AVIITYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSVD 480
           AVIITYKG+HDHD PVPKKRH PPSAP VAAAAPASM+NMQPK TD  ++QISSTQWSVD
Sbjct: 421 AVIITYKGVHDHDMPVPKKRHSPPSAPHVAAAAPASMSNMQPKTTDLTENQISSTQWSVD 480

Query: 481 AEGELTGEALDLGGEKAMESARTLLSIGFEIKPC 511
           AEGELTGEAL+LGGEKAMESARTLLSIGFEIKPC
Sbjct: 481 AEGELTGEALELGGEKAMESARTLLSIGFEIKPC 506

BLAST of MS002336 vs. NCBI nr
Match: XP_023004739.1 (probable WRKY transcription factor 32 [Cucurbita maxima])

HSP 1 Score: 753.4 bits (1944), Expect = 1.2e-213
Identity = 410/515 (79.61%), Postives = 440/515 (85.44%), Query Frame = 0

Query: 1   MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQPAELRAVSS 60
           MAE EG +++QLGSSKA+ GQ+DDEEEEMEDSE+ESEVELE       E + +ELR  SS
Sbjct: 1   MAEPEGFDSNQLGSSKASAGQQDDEEEEMEDSENESEVELE-------EHKLSELRTGSS 60

Query: 61  VNGTAASGSRPETLAAPSAIQSSENGRSDGSILLSSLSNL-FFSFSYNQPVLSSCNEPLA 120
           V   A  GS  +TLA PSA QSSENGRSDG    S+  +L      +  P  S+      
Sbjct: 61  VTEAAVKGSCSQTLAPPSANQSSENGRSDGLPDNSAAESLEGVELKHQAPSASN------ 120

Query: 121 VEAVQTDQVQEQG----ATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQP 180
           VEA+QTDQVQEQ         GP S+QSPTSVTQSISSSASPSLSE+KL+PKKVHKEC+P
Sbjct: 121 VEAIQTDQVQEQSQLQIIVYNGPDSEQSPTSVTQSISSSASPSLSEYKLAPKKVHKECKP 180

Query: 181 EPSQKNSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIE 240
           EPSQKNSSDR+TV  V NVRTPASDGYNWRKYGQKQVK PKGSRSYYKCTYS CCAKKIE
Sbjct: 181 EPSQKNSSDRKTVFSVPNVRTPASDGYNWRKYGQKQVKIPKGSRSYYKCTYSGCCAKKIE 240

Query: 241 CCDHSGHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPT 300
           CCDHSG VTE+VYKSQHSHDPPRKI+NPKES LVPYVEPVVKKI+ EHS RII+DSDPP 
Sbjct: 241 CCDHSGLVTEVVYKSQHSHDPPRKISNPKESMLVPYVEPVVKKIMTEHSVRIINDSDPPI 300

Query: 301 PSKEPLRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPG 360
            SKEPLRETA V+ERKRQYSNDSDGN+E K+K+EN NE ETK KVKKSS GYSG+PLKPG
Sbjct: 301 SSKEPLRETASVVERKRQYSNDSDGNDESKIKNENDNEYETKQKVKKSSGGYSGTPLKPG 360

Query: 361 KKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENP 420
           KKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENP
Sbjct: 361 KKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENP 420

Query: 421 NAVIITYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSV 480
           + VIITYKG+HDHD PVPKKRHGPPSAPLVAAAAPASM NMQPKKTDAVQSQISSTQWSV
Sbjct: 421 SVVIITYKGVHDHDMPVPKKRHGPPSAPLVAAAAPASMGNMQPKKTDAVQSQISSTQWSV 480

Query: 481 DAEGELTGEALDLGGEKAMESARTLLSIGFEIKPC 511
           DAEGELTGEALDLGGEKAMESARTLLSIGFEIKPC
Sbjct: 481 DAEGELTGEALDLGGEKAMESARTLLSIGFEIKPC 502

BLAST of MS002336 vs. ExPASy Swiss-Prot
Match: P59583 (Probable WRKY transcription factor 32 OS=Arabidopsis thaliana OX=3702 GN=WRKY32 PE=2 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.6e-102
Identity = 223/409 (54.52%), Postives = 269/409 (65.77%), Query Frame = 0

Query: 113 SCNEPLAVEAVQTDQVQEQGATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVH-K 172
           S  E +    V  D+V+E          + SP+    S S + +P LS   L P      
Sbjct: 83  SVKETVVSAIVPVDEVEENRQV------ETSPSLAASSDSLTVTPCLS---LDPATASTA 142

Query: 173 ECQPEPSQKNSSDRRTVSPVVN-------VRTPASDGYNWRKYGQKQVKSPKGSRSYYKC 232
           +  P  S     ++R+ SPVVN        RTPA DGYNWRKYGQKQVKSPKGSRSYY+C
Sbjct: 143 QDLPLVSVPTKQEQRSDSPVVNRLSVTPVPRTPARDGYNWRKYGQKQVKSPKGSRSYYRC 202

Query: 233 TYSECCAKKIECCDHSGHVTEIVYKSQHSHDPPRKIN-NPKESKLVPYVEPVVK-KIIAE 292
           TY+ECCAKKIEC + SG+V EIV K  H+H+PPRK + +P+E ++   + PV +   + E
Sbjct: 203 TYTECCAKKIECSNDSGNVVEIVNKGLHTHEPPRKTSFSPREIRVTTAIRPVSEDDTVVE 262

Query: 293 HSRRIISDSDPPTPSKEPLRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKK 352
               + S SDP   +KE + E+  +++RKR   N            E V EPE K ++KK
Sbjct: 263 ELSIVPSGSDPSASTKEYICESQTLVDRKRHCEN------------EAVEEPEPKRRLKK 322

Query: 353 SSAGYSGSPLKPGKKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGC 412
            ++  S S  KPGKK KFVVHAAGDVGI GDGYRWRKYGQKMVKGNPHPRNYYRCTSAGC
Sbjct: 323 DNSQSSDSVSKPGKKNKFVVHAAGDVGICGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGC 382

Query: 413 PVRKHIESAVENPNAVIITYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTD 472
           PVRKHIE+AVEN  AVIITYKG+H+HD PVPKKRHGPPS+ LVAAAAP SM      +TD
Sbjct: 383 PVRKHIETAVENTKAVIITYKGVHNHDMPVPKKRHGPPSSMLVAAAAPTSMRT----RTD 442

Query: 473 AVQSQISSTQWSVDAEGE-LTGEALDLGGEKAMESARTLLSIGFEIKPC 511
              +  +S+Q SV  E E  + EALD+GGEK MESARTLLSIGFEIK C
Sbjct: 443 DQVNIPTSSQCSVGRESEKQSKEALDVGGEKVMESARTLLSIGFEIKQC 466

BLAST of MS002336 vs. ExPASy Swiss-Prot
Match: Q8S8P5 (Probable WRKY transcription factor 33 OS=Arabidopsis thaliana OX=3702 GN=WRKY33 PE=1 SV=2)

HSP 1 Score: 179.5 bits (454), Expect = 9.7e-44
Identity = 111/313 (35.46%), Postives = 166/313 (53.04%), Query Frame = 0

Query: 131 QGATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPEPSQKNSSDRRTVSP 190
           Q +    P +  + T+ T + +SS   S  + K +  +   + +  P      + + VS 
Sbjct: 122 QSSGVSAPTTTTTTTTTTTTTNSSIFQSQEQQKKNQSEQWSQTETRP------NNQAVSY 181

Query: 191 VVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHSGHVTEIVYKS 250
               +    DGYNWRKYGQKQVK  +  RSYYKCT+  C  KK       G +TEIVYK 
Sbjct: 182 NGREQRKGEDGYNWRKYGQKQVKGSENPRSYYKCTFPNCPTKKKVERSLEGQITEIVYKG 241

Query: 251 QHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTPSKEPLRETAIVLER 310
            H+H  P+       S         V     +H+R+  + SD P  +    +  +  +++
Sbjct: 242 SHNHPKPQSTRRSSSSSST--FHSAVYNASLDHNRQ--ASSDQPNSNNSFHQSDSFGMQQ 301

Query: 311 KRQYSNDSDGNEEF--------KVKDENVNEPETK-LKVKKSSAGYSGSPLKPGKKPKFV 370
           +   ++DS G++EF        + +++  +EPE K  K    + G +G   K  ++P+ V
Sbjct: 302 EDNTTSDSVGDDEFEQGSSIVSRDEEDCGSEPEAKRWKGDNETNGGNGGGSKTVREPRIV 361

Query: 371 VHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVIIT 430
           V    D+ I  DGYRWRKYGQK+VKGNP+PR+YY+CT+ GCPVRKH+E A  +  AVI T
Sbjct: 362 VQTTSDIDILDDGYRWRKYGQKVVKGNPNPRSYYKCTTIGCPVRKHVERASHDMRAVITT 421

Query: 431 YKGIHDHDTPVPK 435
           Y+G H+HD P  +
Sbjct: 422 YEGKHNHDVPAAR 424

BLAST of MS002336 vs. ExPASy Swiss-Prot
Match: Q9XI90 (Probable WRKY transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=WRKY4 PE=1 SV=2)

HSP 1 Score: 172.9 bits (437), Expect = 9.0e-42
Identity = 115/331 (34.74%), Postives = 159/331 (48.04%), Query Frame = 0

Query: 138 PGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPEPSQKNSSDRRTVSPVVNVRTP 197
           P ++  P S  QS SS  +         P       Q E S     + R+  P +NV  P
Sbjct: 176 PQTEYPPPSQVQSFSSGQAQ-------IPTSAPLPAQRETSDVTIIEHRSQQP-LNVDKP 235

Query: 198 ASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHSGHVTEIVYKSQHSHDPP 257
           A DGYNWRKYGQKQVK  +  RSYYKCT   C  KK       G VTEI+YK QH+H+PP
Sbjct: 236 ADDGYNWRKYGQKQVKGSEFPRSYYKCTNPGCPVKKKVERSLDGQVTEIIYKGQHNHEPP 295

Query: 258 RKINNPKESKLVPYVEPVVKKIIAEHSRRIIS-DSDPPTPSKEPLRETAIVLERKRQYSN 317
           +      +          +               ++    +K    E           S 
Sbjct: 296 QNTKRGNKDNTANINGSSINNNRGSSELGASQFQTNSSNKTKREQHEAVSQATTTEHLSE 355

Query: 318 DSDGNE------EFKVKDENVNEPETKLKVKKSSAGYSGSPLKPGKKPKFVVHAAGDVGI 377
            SDG E      + + KDEN  +P+ +    + S     +  +   +P+ +V    +V +
Sbjct: 356 ASDGEEVGNGETDVREKDENEPDPKRRSTEVRISEPAPAASHRTVTEPRIIVQTTSEVDL 415

Query: 378 SGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVIITYKGIHDHDT 437
             DGYRWRKYGQK+VKGNP+PR+YY+CT+ GC VRKH+E A  +P AV+ TY+G H+HD 
Sbjct: 416 LDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCGVRKHVERAATDPKAVVTTYEGKHNHDL 475

Query: 438 PVPK-KRHGPPSAPLVAAAAPASMNNMQPKK 461
           P  K   H   +A L     P  + N+  ++
Sbjct: 476 PAAKSSSHAAAAAQLRPDNRPGGLANLNQQQ 498

BLAST of MS002336 vs. ExPASy Swiss-Prot
Match: Q6VWJ6 (WRKY transcription factor SUSIBA2 OS=Hordeum vulgare OX=4513 GN=WRKY46 PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.6e-41
Identity = 120/323 (37.15%), Postives = 164/323 (50.77%), Query Frame = 0

Query: 176 EPSQKNSSDRRTVSPVVN--VRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKK 235
           EP Q NSSD     P         A DGYNWRKYGQK VK  +  RSYYKCT+  C  KK
Sbjct: 164 EPQQMNSSDNAMQEPQSENVADKSADDGYNWRKYGQKHVKGSENPRSYYKCTHPNCEVKK 223

Query: 236 IECCDHSGHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDP 295
           +      G +TE+VYK +H+H  P+  N       VP  +   +   A  S      S+ 
Sbjct: 224 LLERAVDGLITEVVYKGRHNHPKPQP-NRRLAGGAVPSNQGEERYDGA--SAADDKSSNA 283

Query: 296 PTPSKEPLRETAIVLERKRQYSN---DSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGS 355
            +    P+    +V       S+   D+ G   +   D    E     + K  SAG   +
Sbjct: 284 LSNLANPVHSPGMVEPVPASVSDDDIDAGGGRPYPGDDATEEEDLESKRRKMESAGIDAA 343

Query: 356 PL-KPGKKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIE 415
            + KP ++P+ VV    +V I  DGYRWRKYGQK+VKGNP+PR+YY+CTS GCPVRKH+E
Sbjct: 344 LMGKPNREPRVVVQTVSEVDILDDGYRWRKYGQKVVKGNPNPRSYYKCTSTGCPVRKHVE 403

Query: 416 SAVENPNAVIITYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPK---KTDAVQS 475
            A  +P +VI TY+G H+H+ P  +      SAP +        +NM         A ++
Sbjct: 404 RASHDPKSVITTYEGKHNHEVPAARNATHEMSAPPMKNVVHQINSNMPSSIGGMMRACEA 463

Query: 476 QISSTQWSVDAEGELTGEALDLG 490
           +  + Q+S  AE +    +LDLG
Sbjct: 464 RNYTNQYSQAAETDTV--SLDLG 481

BLAST of MS002336 vs. ExPASy Swiss-Prot
Match: Q93WV0 (Probable WRKY transcription factor 20 OS=Arabidopsis thaliana OX=3702 GN=WRKY20 PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 5.9e-41
Identity = 117/314 (37.26%), Postives = 165/314 (52.55%), Query Frame = 0

Query: 136 KGPGSDQSPTSVTQSISSSA-----SPSLSEHKLSPKKVHKECQPEPSQKNSSDRRTVSP 195
           +G GS  SP+S++ +  SS+     +P       S        Q E  Q + +D R  +P
Sbjct: 147 QGHGSSHSPSSISDAAGSSSELSRPTPPCQMTPTSSDIPAGSDQEESIQTSQNDSRGSTP 206

Query: 196 VVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHSGHVTEIVYKS 255
            +     A DGYNWRKYGQK VK  +  RSYYKCT+  C  KK+    H G +T+I+YK 
Sbjct: 207 SI----LADDGYNWRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERSHDGQITDIIYKG 266

Query: 256 QHSHDPPR--KINN----PKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTPSKEPLRET 315
            H H  P+  + N+     +E +L  Y     +    E    + + S+P   +  P  E 
Sbjct: 267 THDHPKPQPGRRNSGGMAAQEERLDKYPSSTGRD---EKGSGVYNLSNPNEQTGNP--EV 326

Query: 316 AIVLERKRQYSNDSDGNEEFKVKDENVNEPETK---LKVKKSSAGYSGSPL-KPGKKPKF 375
             +        + SD   E    + N +EP+      K ++       +PL KP ++P+ 
Sbjct: 327 PPI--------SASDDGGEAAASNRNKDEPDDDDPFSKRRRMEGAMEITPLVKPIREPRV 386

Query: 376 VVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVII 435
           VV    +V I  DGYRWRKYGQK+V+GNP+PR+YY+CT+ GCPVRKH+E A  +P AVI 
Sbjct: 387 VVQTLSEVDILDDGYRWRKYGQKVVRGNPNPRSYYKCTAHGCPVRKHVERASHDPKAVIT 443

BLAST of MS002336 vs. ExPASy TrEMBL
Match: A0A6J1C1P9 (probable WRKY transcription factor 32 OS=Momordica charantia OX=3673 GN=LOC111007163 PE=4 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 2.4e-271
Identity = 493/510 (96.67%), Postives = 499/510 (97.84%), Query Frame = 0

Query: 1   MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQPAELRAVSS 60
           MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQ AELRAVSS
Sbjct: 1   MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQSAELRAVSS 60

Query: 61  VNGTAASGSRPETLAAPSAIQSSENGRSDGSILLSSLSNLFFSFSYNQPVLSSCNEPLAV 120
           VNGTAASGSRPETLAAPSAIQSSENGRSDG ++ S+  +L    +  +PVLSSCNEPLAV
Sbjct: 61  VNGTAASGSRPETLAAPSAIQSSENGRSDGFLVNSATQSL--QGAELKPVLSSCNEPLAV 120

Query: 121 EAVQTDQVQEQGATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPEPSQK 180
           EAVQTDQVQEQGATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPEPSQK
Sbjct: 121 EAVQTDQVQEQGATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPEPSQK 180

Query: 181 NSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHS 240
           NSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHS
Sbjct: 181 NSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHS 240

Query: 241 GHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTPSKEP 300
           GHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTPSKEP
Sbjct: 241 GHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTPSKEP 300

Query: 301 LRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPGKKPKF 360
           LRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPGKKPKF
Sbjct: 301 LRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPGKKPKF 360

Query: 361 VVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVII 420
           VVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVII
Sbjct: 361 VVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVII 420

Query: 421 TYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSVDAEGE 480
           TYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSVDAEGE
Sbjct: 421 TYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSVDAEGE 480

Query: 481 LTGEALDLGGEKAMESARTLLSIGFEIKPC 511
           LTGEALDLGGEKAMESARTLLSIGFEIKPC
Sbjct: 481 LTGEALDLGGEKAMESARTLLSIGFEIKPC 508

BLAST of MS002336 vs. ExPASy TrEMBL
Match: A0A6J1JLY1 (probable WRKY transcription factor 32 OS=Cucurbita maxima OX=3661 GN=LOC111485771 PE=4 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 8.4e-216
Identity = 411/514 (79.96%), Postives = 444/514 (86.38%), Query Frame = 0

Query: 1   MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQPAELRAVSS 60
           MAER+  ETDQLGSSK T GQEDD EEEMEDSEDESEVELEDG G +SE QP ELR  SS
Sbjct: 1   MAERQCFETDQLGSSKVTAGQEDD-EEEMEDSEDESEVELEDG-GALSELQPIELRIDSS 60

Query: 61  VNGTAASGSRPETLAAPSAIQSSENGRSDGSILLSSLSNLFFSFSYNQPVLSSCNEPLAV 120
           V   AA  S  ETL APSA  SSENG      + S+  +L  +     P  SSC+EPLAV
Sbjct: 61  VTEAAARESPSETLTAPSANHSSENGLP----VDSAAQSLEEAELKRSP--SSCHEPLAV 120

Query: 121 EAVQTDQVQEQG----ATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPE 180
           EA QTD+VQEQ     +  KGP  +QSPTSVTQSISSSASPSLS+HKLSPKKV KEC+PE
Sbjct: 121 EATQTDKVQEQSQLQLSIYKGPDPEQSPTSVTQSISSSASPSLSKHKLSPKKVQKECKPE 180

Query: 181 PSQKNSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIEC 240
           PSQKNSS+ +T S V NVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIEC
Sbjct: 181 PSQKNSSNHKTASSVPNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIEC 240

Query: 241 CDHSGHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTP 300
           CDHSGH TEIVY+SQHSHDPPR I+NPKE +LVPYVEPVVK+II +H+RR+I+DSDPPTP
Sbjct: 241 CDHSGHRTEIVYRSQHSHDPPRMISNPKERQLVPYVEPVVKQIITQHTRRVINDSDPPTP 300

Query: 301 SKEPLRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPGK 360
           SKEP RETA+VL RKRQYS+DSDGN+EFK+KDEN  EPETK KVKKSSAGYSG+PLKPGK
Sbjct: 301 SKEPSRETALVLVRKRQYSSDSDGNDEFKIKDENDGEPETKQKVKKSSAGYSGTPLKPGK 360

Query: 361 KPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPN 420
           KPKFVVHAAGDVGISGDGYRWRKYGQKMVKG+PHPRNYYRCTSAGCPVRKHIESAVENP 
Sbjct: 361 KPKFVVHAAGDVGISGDGYRWRKYGQKMVKGSPHPRNYYRCTSAGCPVRKHIESAVENPY 420

Query: 421 AVIITYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSVD 480
           AVIITYKG+HDHD PVPKKRH PPSAP VAAAAPASM+N QPKKT+  +SQISSTQWSVD
Sbjct: 421 AVIITYKGVHDHDMPVPKKRHSPPSAPRVAAAAPASMSNTQPKKTNLTESQISSTQWSVD 480

Query: 481 AEGELTGEALDLGGEKAMESARTLLSIGFEIKPC 511
           AEGELTGEAL+LGGEKAMESARTLLSIGFEIKPC
Sbjct: 481 AEGELTGEALELGGEKAMESARTLLSIGFEIKPC 506

BLAST of MS002336 vs. ExPASy TrEMBL
Match: A0A6J1KX67 (probable WRKY transcription factor 32 OS=Cucurbita maxima OX=3661 GN=LOC111497950 PE=4 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 6.0e-214
Identity = 410/515 (79.61%), Postives = 440/515 (85.44%), Query Frame = 0

Query: 1   MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQPAELRAVSS 60
           MAE EG +++QLGSSKA+ GQ+DDEEEEMEDSE+ESEVELE       E + +ELR  SS
Sbjct: 1   MAEPEGFDSNQLGSSKASAGQQDDEEEEMEDSENESEVELE-------EHKLSELRTGSS 60

Query: 61  VNGTAASGSRPETLAAPSAIQSSENGRSDGSILLSSLSNL-FFSFSYNQPVLSSCNEPLA 120
           V   A  GS  +TLA PSA QSSENGRSDG    S+  +L      +  P  S+      
Sbjct: 61  VTEAAVKGSCSQTLAPPSANQSSENGRSDGLPDNSAAESLEGVELKHQAPSASN------ 120

Query: 121 VEAVQTDQVQEQG----ATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQP 180
           VEA+QTDQVQEQ         GP S+QSPTSVTQSISSSASPSLSE+KL+PKKVHKEC+P
Sbjct: 121 VEAIQTDQVQEQSQLQIIVYNGPDSEQSPTSVTQSISSSASPSLSEYKLAPKKVHKECKP 180

Query: 181 EPSQKNSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIE 240
           EPSQKNSSDR+TV  V NVRTPASDGYNWRKYGQKQVK PKGSRSYYKCTYS CCAKKIE
Sbjct: 181 EPSQKNSSDRKTVFSVPNVRTPASDGYNWRKYGQKQVKIPKGSRSYYKCTYSGCCAKKIE 240

Query: 241 CCDHSGHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPT 300
           CCDHSG VTE+VYKSQHSHDPPRKI+NPKES LVPYVEPVVKKI+ EHS RII+DSDPP 
Sbjct: 241 CCDHSGLVTEVVYKSQHSHDPPRKISNPKESMLVPYVEPVVKKIMTEHSVRIINDSDPPI 300

Query: 301 PSKEPLRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPG 360
            SKEPLRETA V+ERKRQYSNDSDGN+E K+K+EN NE ETK KVKKSS GYSG+PLKPG
Sbjct: 301 SSKEPLRETASVVERKRQYSNDSDGNDESKIKNENDNEYETKQKVKKSSGGYSGTPLKPG 360

Query: 361 KKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENP 420
           KKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENP
Sbjct: 361 KKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENP 420

Query: 421 NAVIITYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSV 480
           + VIITYKG+HDHD PVPKKRHGPPSAPLVAAAAPASM NMQPKKTDAVQSQISSTQWSV
Sbjct: 421 SVVIITYKGVHDHDMPVPKKRHGPPSAPLVAAAAPASMGNMQPKKTDAVQSQISSTQWSV 480

Query: 481 DAEGELTGEALDLGGEKAMESARTLLSIGFEIKPC 511
           DAEGELTGEALDLGGEKAMESARTLLSIGFEIKPC
Sbjct: 481 DAEGELTGEALDLGGEKAMESARTLLSIGFEIKPC 502

BLAST of MS002336 vs. ExPASy TrEMBL
Match: A0A6J1F0K1 (probable WRKY transcription factor 32 OS=Cucurbita moschata OX=3662 GN=LOC111438384 PE=4 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 2.5e-212
Identity = 408/515 (79.22%), Postives = 440/515 (85.44%), Query Frame = 0

Query: 1   MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQPAELRAVSS 60
           MAE EG +++QLGSSKA+  Q+DDEEEEMEDSE+ESEVELE       E + +ELR  S 
Sbjct: 1   MAEPEGFDSNQLGSSKASAEQQDDEEEEMEDSENESEVELE-------EHKLSELRTGSL 60

Query: 61  VNGTAASGSRPETLAAPSAIQSSENGRSDGSILLSSLSNL-FFSFSYNQPVLSSCNEPLA 120
           V+  A  GS  +TLA PSA QSSENGRSDG    S+  +L      +  P  S+      
Sbjct: 61  VSEAAVKGSCSQTLAPPSANQSSENGRSDGLPNNSAAESLEGVELKHQAPSASN------ 120

Query: 121 VEAVQTDQVQEQG----ATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQP 180
           VEA+QTDQVQEQ        KGP S+QSPTSVTQSISSSASPSLSE+KL PKKVH+EC+P
Sbjct: 121 VEAIQTDQVQEQSQLQIIVYKGPDSEQSPTSVTQSISSSASPSLSEYKLVPKKVHEECKP 180

Query: 181 EPSQKNSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIE 240
           EPSQKNSSDR+TV  V NVRTPASDGYNWRKYGQKQVK PKGSRSYYKCTYS CCAKKIE
Sbjct: 181 EPSQKNSSDRKTVFSVPNVRTPASDGYNWRKYGQKQVKIPKGSRSYYKCTYSGCCAKKIE 240

Query: 241 CCDHSGHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPT 300
           CCDHSG VTE+VYKSQHSHDPPRKI+NPKES LVPYVEPVVKKI+AEHS RII+DSDPP 
Sbjct: 241 CCDHSGLVTEVVYKSQHSHDPPRKISNPKESMLVPYVEPVVKKIMAEHSVRIINDSDPPM 300

Query: 301 PSKEPLRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPG 360
            SKEPLRETA V+ERKRQ+SNDSDGN+E K+K+EN NE ETK KVKKSS GYSG+PLKPG
Sbjct: 301 SSKEPLRETASVVERKRQFSNDSDGNDESKIKNENDNEYETKQKVKKSSGGYSGTPLKPG 360

Query: 361 KKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENP 420
           KKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENP
Sbjct: 361 KKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENP 420

Query: 421 NAVIITYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSV 480
           + VIITYKG+HDHD PVPKKRHGPPSAPLVAAAAPASM NMQPKKTDAVQSQISSTQWSV
Sbjct: 421 SVVIITYKGVHDHDMPVPKKRHGPPSAPLVAAAAPASMGNMQPKKTDAVQSQISSTQWSV 480

Query: 481 DAEGELTGEALDLGGEKAMESARTLLSIGFEIKPC 511
           DAEGELTGEALDLGGEKAMESARTLLSIGFEIKPC
Sbjct: 481 DAEGELTGEALDLGGEKAMESARTLLSIGFEIKPC 502

BLAST of MS002336 vs. ExPASy TrEMBL
Match: A0A6J1E1D3 (probable WRKY transcription factor 32 OS=Cucurbita moschata OX=3662 GN=LOC111429894 PE=4 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 4.4e-209
Identity = 404/514 (78.60%), Postives = 433/514 (84.24%), Query Frame = 0

Query: 1   MAEREGCETDQLGSSKATGGQEDDEEEEMEDSEDESEVELEDGEGRVSESQPAELRAVSS 60
           MAER+  ETD+LGSSK T GQEDD EEEMED    SEVELEDGE  +SE QP E R  S 
Sbjct: 1   MAERQCFETDRLGSSKVTAGQEDD-EEEMED----SEVELEDGEA-LSELQPIESRIDSL 60

Query: 61  VNGTAASGSRPETLAAPSAIQSSENGRSDGSILLSSLSNLFFSFSYNQPVLSSCNEPLAV 120
           V   A   S  ETL APSA  SSENG    S    SL    F  S      SSC+EPLAV
Sbjct: 61  VTEAAVRESPSETLTAPSANHSSENGLPVDS-AAQSLEEAEFKQS-----PSSCHEPLAV 120

Query: 121 EAVQTDQVQEQG----ATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPE 180
           EA +TD+VQEQ     +  KGP  +QSPTSVTQSISSSASPSLS+HKLSPKKV KEC+PE
Sbjct: 121 EATETDKVQEQSQLQLSIYKGPDPEQSPTSVTQSISSSASPSLSKHKLSPKKVQKECKPE 180

Query: 181 PSQKNSSDRRTVSPVVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIEC 240
           PSQKNSS+ +T S V NVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIEC
Sbjct: 181 PSQKNSSNHKTASSVPNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIEC 240

Query: 241 CDHSGHVTEIVYKSQHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTP 300
           CDHSGH TEIVY+SQHSHDPPR I+NPKE +LVPYVEPVVK+II EHSRR+I+DS+PPTP
Sbjct: 241 CDHSGHRTEIVYRSQHSHDPPRMISNPKERQLVPYVEPVVKQIITEHSRRVINDSNPPTP 300

Query: 301 SKEPLRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKKSSAGYSGSPLKPGK 360
           SKEP RETA VL RKRQYS+DSDGN+EFK+KDEN  EPETK KVKKSSAGYSG+PLKPGK
Sbjct: 301 SKEPSRETASVLVRKRQYSSDSDGNDEFKIKDENDGEPETKQKVKKSSAGYSGTPLKPGK 360

Query: 361 KPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPN 420
           KPKFVVHAAGDVGISGDGYRWRKYGQKMVKG+PHPRNYYRCTSAGCPVRKHIESAVENP 
Sbjct: 361 KPKFVVHAAGDVGISGDGYRWRKYGQKMVKGSPHPRNYYRCTSAGCPVRKHIESAVENPY 420

Query: 421 AVIITYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTDAVQSQISSTQWSVD 480
           AVIITYKG+HDHD PVPKKRH PPSAP VAAAAPASM+N QPK TD  ++QISSTQWSVD
Sbjct: 421 AVIITYKGVHDHDMPVPKKRHSPPSAPHVAAAAPASMSNTQPKTTDLTENQISSTQWSVD 480

Query: 481 AEGELTGEALDLGGEKAMESARTLLSIGFEIKPC 511
           AEGELTGEAL+LGGEKAMESARTLLSIGFEIKPC
Sbjct: 481 AEGELTGEALELGGEKAMESARTLLSIGFEIKPC 502

BLAST of MS002336 vs. TAIR 10
Match: AT4G30935.1 (WRKY DNA-binding protein 32 )

HSP 1 Score: 374.8 bits (961), Expect = 1.1e-103
Identity = 223/409 (54.52%), Postives = 269/409 (65.77%), Query Frame = 0

Query: 113 SCNEPLAVEAVQTDQVQEQGATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVH-K 172
           S  E +    V  D+V+E          + SP+    S S + +P LS   L P      
Sbjct: 83  SVKETVVSAIVPVDEVEENRQV------ETSPSLAASSDSLTVTPCLS---LDPATASTA 142

Query: 173 ECQPEPSQKNSSDRRTVSPVVN-------VRTPASDGYNWRKYGQKQVKSPKGSRSYYKC 232
           +  P  S     ++R+ SPVVN        RTPA DGYNWRKYGQKQVKSPKGSRSYY+C
Sbjct: 143 QDLPLVSVPTKQEQRSDSPVVNRLSVTPVPRTPARDGYNWRKYGQKQVKSPKGSRSYYRC 202

Query: 233 TYSECCAKKIECCDHSGHVTEIVYKSQHSHDPPRKIN-NPKESKLVPYVEPVVK-KIIAE 292
           TY+ECCAKKIEC + SG+V EIV K  H+H+PPRK + +P+E ++   + PV +   + E
Sbjct: 203 TYTECCAKKIECSNDSGNVVEIVNKGLHTHEPPRKTSFSPREIRVTTAIRPVSEDDTVVE 262

Query: 293 HSRRIISDSDPPTPSKEPLRETAIVLERKRQYSNDSDGNEEFKVKDENVNEPETKLKVKK 352
               + S SDP   +KE + E+  +++RKR   N            E V EPE K ++KK
Sbjct: 263 ELSIVPSGSDPSASTKEYICESQTLVDRKRHCEN------------EAVEEPEPKRRLKK 322

Query: 353 SSAGYSGSPLKPGKKPKFVVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGC 412
            ++  S S  KPGKK KFVVHAAGDVGI GDGYRWRKYGQKMVKGNPHPRNYYRCTSAGC
Sbjct: 323 DNSQSSDSVSKPGKKNKFVVHAAGDVGICGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGC 382

Query: 413 PVRKHIESAVENPNAVIITYKGIHDHDTPVPKKRHGPPSAPLVAAAAPASMNNMQPKKTD 472
           PVRKHIE+AVEN  AVIITYKG+H+HD PVPKKRHGPPS+ LVAAAAP SM      +TD
Sbjct: 383 PVRKHIETAVENTKAVIITYKGVHNHDMPVPKKRHGPPSSMLVAAAAPTSMRT----RTD 442

Query: 473 AVQSQISSTQWSVDAEGE-LTGEALDLGGEKAMESARTLLSIGFEIKPC 511
              +  +S+Q SV  E E  + EALD+GGEK MESARTLLSIGFEIK C
Sbjct: 443 DQVNIPTSSQCSVGRESEKQSKEALDVGGEKVMESARTLLSIGFEIKQC 466

BLAST of MS002336 vs. TAIR 10
Match: AT2G38470.1 (WRKY DNA-binding protein 33 )

HSP 1 Score: 179.5 bits (454), Expect = 6.9e-45
Identity = 111/313 (35.46%), Postives = 166/313 (53.04%), Query Frame = 0

Query: 131 QGATCKGPGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPEPSQKNSSDRRTVSP 190
           Q +    P +  + T+ T + +SS   S  + K +  +   + +  P      + + VS 
Sbjct: 122 QSSGVSAPTTTTTTTTTTTTTNSSIFQSQEQQKKNQSEQWSQTETRP------NNQAVSY 181

Query: 191 VVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHSGHVTEIVYKS 250
               +    DGYNWRKYGQKQVK  +  RSYYKCT+  C  KK       G +TEIVYK 
Sbjct: 182 NGREQRKGEDGYNWRKYGQKQVKGSENPRSYYKCTFPNCPTKKKVERSLEGQITEIVYKG 241

Query: 251 QHSHDPPRKINNPKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTPSKEPLRETAIVLER 310
            H+H  P+       S         V     +H+R+  + SD P  +    +  +  +++
Sbjct: 242 SHNHPKPQSTRRSSSSSST--FHSAVYNASLDHNRQ--ASSDQPNSNNSFHQSDSFGMQQ 301

Query: 311 KRQYSNDSDGNEEF--------KVKDENVNEPETK-LKVKKSSAGYSGSPLKPGKKPKFV 370
           +   ++DS G++EF        + +++  +EPE K  K    + G +G   K  ++P+ V
Sbjct: 302 EDNTTSDSVGDDEFEQGSSIVSRDEEDCGSEPEAKRWKGDNETNGGNGGGSKTVREPRIV 361

Query: 371 VHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVIIT 430
           V    D+ I  DGYRWRKYGQK+VKGNP+PR+YY+CT+ GCPVRKH+E A  +  AVI T
Sbjct: 362 VQTTSDIDILDDGYRWRKYGQKVVKGNPNPRSYYKCTTIGCPVRKHVERASHDMRAVITT 421

Query: 431 YKGIHDHDTPVPK 435
           Y+G H+HD P  +
Sbjct: 422 YEGKHNHDVPAAR 424

BLAST of MS002336 vs. TAIR 10
Match: AT1G13960.1 (WRKY DNA-binding protein 4 )

HSP 1 Score: 172.9 bits (437), Expect = 6.4e-43
Identity = 115/331 (34.74%), Postives = 159/331 (48.04%), Query Frame = 0

Query: 138 PGSDQSPTSVTQSISSSASPSLSEHKLSPKKVHKECQPEPSQKNSSDRRTVSPVVNVRTP 197
           P ++  P S  QS SS  +         P       Q E S     + R+  P +NV  P
Sbjct: 176 PQTEYPPPSQVQSFSSGQAQ-------IPTSAPLPAQRETSDVTIIEHRSQQP-LNVDKP 235

Query: 198 ASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHSGHVTEIVYKSQHSHDPP 257
           A DGYNWRKYGQKQVK  +  RSYYKCT   C  KK       G VTEI+YK QH+H+PP
Sbjct: 236 ADDGYNWRKYGQKQVKGSEFPRSYYKCTNPGCPVKKKVERSLDGQVTEIIYKGQHNHEPP 295

Query: 258 RKINNPKESKLVPYVEPVVKKIIAEHSRRIIS-DSDPPTPSKEPLRETAIVLERKRQYSN 317
           +      +          +               ++    +K    E           S 
Sbjct: 296 QNTKRGNKDNTANINGSSINNNRGSSELGASQFQTNSSNKTKREQHEAVSQATTTEHLSE 355

Query: 318 DSDGNE------EFKVKDENVNEPETKLKVKKSSAGYSGSPLKPGKKPKFVVHAAGDVGI 377
            SDG E      + + KDEN  +P+ +    + S     +  +   +P+ +V    +V +
Sbjct: 356 ASDGEEVGNGETDVREKDENEPDPKRRSTEVRISEPAPAASHRTVTEPRIIVQTTSEVDL 415

Query: 378 SGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVIITYKGIHDHDT 437
             DGYRWRKYGQK+VKGNP+PR+YY+CT+ GC VRKH+E A  +P AV+ TY+G H+HD 
Sbjct: 416 LDDGYRWRKYGQKVVKGNPYPRSYYKCTTPGCGVRKHVERAATDPKAVVTTYEGKHNHDL 475

Query: 438 PVPK-KRHGPPSAPLVAAAAPASMNNMQPKK 461
           P  K   H   +A L     P  + N+  ++
Sbjct: 476 PAAKSSSHAAAAAQLRPDNRPGGLANLNQQQ 498

BLAST of MS002336 vs. TAIR 10
Match: AT4G26640.1 (WRKY family transcription factor family protein )

HSP 1 Score: 170.2 bits (430), Expect = 4.2e-42
Identity = 117/314 (37.26%), Postives = 165/314 (52.55%), Query Frame = 0

Query: 136 KGPGSDQSPTSVTQSISSSA-----SPSLSEHKLSPKKVHKECQPEPSQKNSSDRRTVSP 195
           +G GS  SP+S++ +  SS+     +P       S        Q E  Q + +D R  +P
Sbjct: 75  QGHGSSHSPSSISDAAGSSSELSRPTPPCQMTPTSSDIPAGSDQEESIQTSQNDSRGSTP 134

Query: 196 VVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHSGHVTEIVYKS 255
            +     A DGYNWRKYGQK VK  +  RSYYKCT+  C  KK+    H G +T+I+YK 
Sbjct: 135 SI----LADDGYNWRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERSHDGQITDIIYKG 194

Query: 256 QHSHDPPR--KINN----PKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTPSKEPLRET 315
            H H  P+  + N+     +E +L  Y     +    E    + + S+P   +  P  E 
Sbjct: 195 THDHPKPQPGRRNSGGMAAQEERLDKYPSSTGRD---EKGSGVYNLSNPNEQTGNP--EV 254

Query: 316 AIVLERKRQYSNDSDGNEEFKVKDENVNEPETK---LKVKKSSAGYSGSPL-KPGKKPKF 375
             +        + SD   E    + N +EP+      K ++       +PL KP ++P+ 
Sbjct: 255 PPI--------SASDDGGEAAASNRNKDEPDDDDPFSKRRRMEGAMEITPLVKPIREPRV 314

Query: 376 VVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVII 435
           VV    +V I  DGYRWRKYGQK+V+GNP+PR+YY+CT+ GCPVRKH+E A  +P AVI 
Sbjct: 315 VVQTLSEVDILDDGYRWRKYGQKVVRGNPNPRSYYKCTAHGCPVRKHVERASHDPKAVIT 371

BLAST of MS002336 vs. TAIR 10
Match: AT4G26640.2 (WRKY family transcription factor family protein )

HSP 1 Score: 170.2 bits (430), Expect = 4.2e-42
Identity = 117/314 (37.26%), Postives = 165/314 (52.55%), Query Frame = 0

Query: 136 KGPGSDQSPTSVTQSISSSA-----SPSLSEHKLSPKKVHKECQPEPSQKNSSDRRTVSP 195
           +G GS  SP+S++ +  SS+     +P       S        Q E  Q + +D R  +P
Sbjct: 147 QGHGSSHSPSSISDAAGSSSELSRPTPPCQMTPTSSDIPAGSDQEESIQTSQNDSRGSTP 206

Query: 196 VVNVRTPASDGYNWRKYGQKQVKSPKGSRSYYKCTYSECCAKKIECCDHSGHVTEIVYKS 255
            +     A DGYNWRKYGQK VK  +  RSYYKCT+  C  KK+    H G +T+I+YK 
Sbjct: 207 SI----LADDGYNWRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERSHDGQITDIIYKG 266

Query: 256 QHSHDPPR--KINN----PKESKLVPYVEPVVKKIIAEHSRRIISDSDPPTPSKEPLRET 315
            H H  P+  + N+     +E +L  Y     +    E    + + S+P   +  P  E 
Sbjct: 267 THDHPKPQPGRRNSGGMAAQEERLDKYPSSTGRD---EKGSGVYNLSNPNEQTGNP--EV 326

Query: 316 AIVLERKRQYSNDSDGNEEFKVKDENVNEPETK---LKVKKSSAGYSGSPL-KPGKKPKF 375
             +        + SD   E    + N +EP+      K ++       +PL KP ++P+ 
Sbjct: 327 PPI--------SASDDGGEAAASNRNKDEPDDDDPFSKRRRMEGAMEITPLVKPIREPRV 386

Query: 376 VVHAAGDVGISGDGYRWRKYGQKMVKGNPHPRNYYRCTSAGCPVRKHIESAVENPNAVII 435
           VV    +V I  DGYRWRKYGQK+V+GNP+PR+YY+CT+ GCPVRKH+E A  +P AVI 
Sbjct: 387 VVQTLSEVDILDDGYRWRKYGQKVVRGNPNPRSYYKCTAHGCPVRKHVERASHDPKAVIT 443

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135102.14.9e-27196.67probable WRKY transcription factor 32 [Momordica charantia][more]
XP_038879508.19.2e-21781.04probable WRKY transcription factor 32 [Benincasa hispida][more]
XP_022988569.11.7e-21579.96probable WRKY transcription factor 32 [Cucurbita maxima][more]
KAG6589772.14.3e-21479.57putative WRKY transcription factor 32, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023004739.11.2e-21379.61probable WRKY transcription factor 32 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P595831.6e-10254.52Probable WRKY transcription factor 32 OS=Arabidopsis thaliana OX=3702 GN=WRKY32 ... [more]
Q8S8P59.7e-4435.46Probable WRKY transcription factor 33 OS=Arabidopsis thaliana OX=3702 GN=WRKY33 ... [more]
Q9XI909.0e-4234.74Probable WRKY transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=WRKY4 PE... [more]
Q6VWJ62.6e-4137.15WRKY transcription factor SUSIBA2 OS=Hordeum vulgare OX=4513 GN=WRKY46 PE=1 SV=1[more]
Q93WV05.9e-4137.26Probable WRKY transcription factor 20 OS=Arabidopsis thaliana OX=3702 GN=WRKY20 ... [more]
Match NameE-valueIdentityDescription
A0A6J1C1P92.4e-27196.67probable WRKY transcription factor 32 OS=Momordica charantia OX=3673 GN=LOC11100... [more]
A0A6J1JLY18.4e-21679.96probable WRKY transcription factor 32 OS=Cucurbita maxima OX=3661 GN=LOC11148577... [more]
A0A6J1KX676.0e-21479.61probable WRKY transcription factor 32 OS=Cucurbita maxima OX=3661 GN=LOC11149795... [more]
A0A6J1F0K12.5e-21279.22probable WRKY transcription factor 32 OS=Cucurbita moschata OX=3662 GN=LOC111438... [more]
A0A6J1E1D34.4e-20978.60probable WRKY transcription factor 32 OS=Cucurbita moschata OX=3662 GN=LOC111429... [more]
Match NameE-valueIdentityDescription
AT4G30935.11.1e-10354.52WRKY DNA-binding protein 32 [more]
AT2G38470.16.9e-4535.46WRKY DNA-binding protein 33 [more]
AT1G13960.16.4e-4334.74WRKY DNA-binding protein 4 [more]
AT4G26640.14.2e-4237.26WRKY family transcription factor family protein [more]
AT4G26640.24.2e-4237.26WRKY family transcription factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 22..42
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 163..179
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..91
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..331
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..202
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..91
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 126..161
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 180..196
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..42
NoneNo IPR availablePANTHERPTHR31221:SF150WRKY TRANSCRIPTION FACTOR 32-RELATEDcoord: 24..508
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 371..430
e-value: 5.2E-35
score: 132.3
coord: 198..256
e-value: 4.1E-23
score: 92.8
IPR003657WRKY domainPFAMPF03106WRKYcoord: 373..428
e-value: 1.0E-23
score: 83.4
coord: 200..254
e-value: 1.2E-16
score: 60.7
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 199..257
score: 17.212719
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 366..431
score: 32.110386
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 184..258
e-value: 1.9E-19
score: 71.2
coord: 356..432
e-value: 3.1E-29
score: 102.6
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 367..431
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 196..257
IPR044810WRKY transcription factor, plantPANTHERPTHR31221WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATEDcoord: 24..508

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002336.1MS002336.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding