MS002321 (gene) Bitter gourd (TR) v1

Overview
NameMS002321
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationscaffold30: 4135502 .. 4137634 (-)
RNA-Seq ExpressionMS002321
SyntenyMS002321
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTATTCATGTTCAATGTCCTTATAAGGGGCTTCTCGGACAATAACTTGCCCAAATCTTCGATCTCTATCTATACCCATTTGAGAAAAAGGACTAATCTAAGGCCAGACAATTTCACTTATGCATTTGCTATATCAGCTGCTTCGAGGTTTGAAGATGAAAGGATTGGCACCTTGTTGCACGCACATTCAATTGTTGATGGTGTGGCATCGAACTTGTTTGTTGGGTCTGCGACTGTTGATTTATACTTTAAATTTATGCGCGCGGACTTGGCGCGGAAGGTGTTTGATGTAATACCTGAGCGAGATACGGTTTTGTGGAACACGATGATATCCGGGTTTTCTAGGAACTCATGTTTTGAGGACTCGATTCGTGTTTTTGCAGATATGCTTGACGTTGGGCTGCCATTTGATTCTACAACTTTGGCTGCGGTGCTTACAGCAGTGGCGGAGTTGCAGGAATATAGATTAGGGATGAGTATTCAATGTTTGGCTTCCAAAAAAGGGTTCCATTATGATGTCTACGTGCTTACAGGTTTGATATCATTGTACTCAAAATGTGGGGAGAGTGACAAAGGAAGGTCGTTGTTTGATCAGATTGGTCAGCCAGATTTGATATCTTATAACGCAATGATTTCTGGTTATACTTTCAATCATGAAACTGGGTCGGCAGTGGCACTCTTCAGGGAATTGCTTGCTTCCGGACAGGAAGTTAATTCAAGCACATTGGTGGGCTTAATTCCAGTTTTTTCGCCCTTTGGCCATCTGCAGCTTACTTGCTCTATTCAAACTTTAAGCACGAAGATTGGTATTGTTTCACAACCTTCTGTTTCAACTGCACTCACTACTGTTTACTGTCGACTAAACGAAATTCAATGTGCAAGGCAGCTGTTTGATGAATCTTCGGAGAAAAGTTTGGCTTCCTGGAATGCCATGATATCAGGATACACTCAAAATGGGTTGACAGAGAGAGCAATTTCTCTTTTCCAGGAAATGATGCCTCAACTCAGTCCAAATCCTGTCACCATTACCAGTGTACTTTCGGCTTGCGCTCAGCTTGGAGCTCTAAGTATTGGCAAATGGGTCCATGGATTGATCAAGAGTGAAAAACTTGAATCAAATGTATATGTTGCAACTGCTTTAGTTGATATGCATGCAAAGTGTGGTAGCATTGCAGAGGCTCGACAGTTATTTGACATGATGGCAGAAAAGAATGTGGTAACTTGGAATGCTATGATAACTGGTTATGGTCTTCATGGATATGGAAAAGAAGCACTAAACCTCTTTAATGAGATGTTGCAATCAGGGACTCCACCGACTAGTGTTACTTTTCTATCTATCTTGTATGCTTGCAGTCATTCTGGCTTGGTGAGAGAGGGTAATGAAATTTTCCACTCTATGGTTAACAATTATGGTTTTCAGCCCATGAGCGAGCACTATGCTTGCATGGTCGACATACTTGGGAGAGCTGGACAGCTAAAGAATGCCTTGGAGTTCATTGAAAGAATGCCACTTGAGCCCGGCCCTGCTGTTTGGGGTGCATTGCTTGGTGCCTGCATGATTCACAAGAATACAGACATAGCCCATGTTGCTTCAGAAAGACTTTTCCAATTGGACCCAGAAAATGTAGGATACTATGTCCTGCTTTCAAATATTTATTCTGCTGATAGGAACTTCCCCAAAGCTGCTTCTGTCCGACAAGTTGTTAAGAAGAGAAAACTGGCAAAGACGCCAGGGTGCACTCTTATTGAGATCAGTAATCAACCATATGTTTTCACATCTGGTGATCAGTCCCATCCTCAGGCCACAGCCATTTATGCCATGCTAGAGAAGTTAGCAGGAAAGATGAGAGAGGCTGGATATCAATCGGAGACTGTTACTGCTTTGCATGATGTAGAGGATGAAGAGAAGGAGTTAATGGTGAATGTCCACAGTGAAAAATTAGCAATTGCTTTTGGGCTCATTTCAACTGAACCTGGAACTGAAATTAGGATTATCAAGAACCTCCGAGTTTGTTTAGACTGTCATACTGTAACTAAATTTATATCTAAGATCACAGAGAGAGTGATTGTGGTCAGGGATGCTAATAGGTTCCATCATTTCAAAAATGGTATTTGTTCATGTGGAGACTATTGG

mRNA sequence

CTATTCATGTTCAATGTCCTTATAAGGGGCTTCTCGGACAATAACTTGCCCAAATCTTCGATCTCTATCTATACCCATTTGAGAAAAAGGACTAATCTAAGGCCAGACAATTTCACTTATGCATTTGCTATATCAGCTGCTTCGAGGTTTGAAGATGAAAGGATTGGCACCTTGTTGCACGCACATTCAATTGTTGATGGTGTGGCATCGAACTTGTTTGTTGGGTCTGCGACTGTTGATTTATACTTTAAATTTATGCGCGCGGACTTGGCGCGGAAGGTGTTTGATGTAATACCTGAGCGAGATACGGTTTTGTGGAACACGATGATATCCGGGTTTTCTAGGAACTCATGTTTTGAGGACTCGATTCGTGTTTTTGCAGATATGCTTGACGTTGGGCTGCCATTTGATTCTACAACTTTGGCTGCGGTGCTTACAGCAGTGGCGGAGTTGCAGGAATATAGATTAGGGATGAGTATTCAATGTTTGGCTTCCAAAAAAGGGTTCCATTATGATGTCTACGTGCTTACAGGTTTGATATCATTGTACTCAAAATGTGGGGAGAGTGACAAAGGAAGGTCGTTGTTTGATCAGATTGGTCAGCCAGATTTGATATCTTATAACGCAATGATTTCTGGTTATACTTTCAATCATGAAACTGGGTCGGCAGTGGCACTCTTCAGGGAATTGCTTGCTTCCGGACAGGAAGTTAATTCAAGCACATTGGTGGGCTTAATTCCAGTTTTTTCGCCCTTTGGCCATCTGCAGCTTACTTGCTCTATTCAAACTTTAAGCACGAAGATTGGTATTGTTTCACAACCTTCTGTTTCAACTGCACTCACTACTGTTTACTGTCGACTAAACGAAATTCAATGTGCAAGGCAGCTGTTTGATGAATCTTCGGAGAAAAGTTTGGCTTCCTGGAATGCCATGATATCAGGATACACTCAAAATGGGTTGACAGAGAGAGCAATTTCTCTTTTCCAGGAAATGATGCCTCAACTCAGTCCAAATCCTGTCACCATTACCAGTGTACTTTCGGCTTGCGCTCAGCTTGGAGCTCTAAGTATTGGCAAATGGGTCCATGGATTGATCAAGAGTGAAAAACTTGAATCAAATGTATATGTTGCAACTGCTTTAGTTGATATGCATGCAAAGTGTGGTAGCATTGCAGAGGCTCGACAGTTATTTGACATGATGGCAGAAAAGAATGTGGTAACTTGGAATGCTATGATAACTGGTTATGGTCTTCATGGATATGGAAAAGAAGCACTAAACCTCTTTAATGAGATGTTGCAATCAGGGACTCCACCGACTAGTGTTACTTTTCTATCTATCTTGTATGCTTGCAGTCATTCTGGCTTGGTGAGAGAGGGTAATGAAATTTTCCACTCTATGGTTAACAATTATGGTTTTCAGCCCATGAGCGAGCACTATGCTTGCATGGTCGACATACTTGGGAGAGCTGGACAGCTAAAGAATGCCTTGGAGTTCATTGAAAGAATGCCACTTGAGCCCGGCCCTGCTGTTTGGGGTGCATTGCTTGGTGCCTGCATGATTCACAAGAATACAGACATAGCCCATGTTGCTTCAGAAAGACTTTTCCAATTGGACCCAGAAAATGTAGGATACTATGTCCTGCTTTCAAATATTTATTCTGCTGATAGGAACTTCCCCAAAGCTGCTTCTGTCCGACAAGTTGTTAAGAAGAGAAAACTGGCAAAGACGCCAGGGTGCACTCTTATTGAGATCAGTAATCAACCATATGTTTTCACATCTGGTGATCAGTCCCATCCTCAGGCCACAGCCATTTATGCCATGCTAGAGAAGTTAGCAGGAAAGATGAGAGAGGCTGGATATCAATCGGAGACTGTTACTGCTTTGCATGATGTAGAGGATGAAGAGAAGGAGTTAATGGTGAATGTCCACAGTGAAAAATTAGCAATTGCTTTTGGGCTCATTTCAACTGAACCTGGAACTGAAATTAGGATTATCAAGAACCTCCGAGTTTGTTTAGACTGTCATACTGTAACTAAATTTATATCTAAGATCACAGAGAGAGTGATTGTGGTCAGGGATGCTAATAGGTTCCATCATTTCAAAAATGGTATTTGTTCATGTGGAGACTATTGG

Coding sequence (CDS)

CTATTCATGTTCAATGTCCTTATAAGGGGCTTCTCGGACAATAACTTGCCCAAATCTTCGATCTCTATCTATACCCATTTGAGAAAAAGGACTAATCTAAGGCCAGACAATTTCACTTATGCATTTGCTATATCAGCTGCTTCGAGGTTTGAAGATGAAAGGATTGGCACCTTGTTGCACGCACATTCAATTGTTGATGGTGTGGCATCGAACTTGTTTGTTGGGTCTGCGACTGTTGATTTATACTTTAAATTTATGCGCGCGGACTTGGCGCGGAAGGTGTTTGATGTAATACCTGAGCGAGATACGGTTTTGTGGAACACGATGATATCCGGGTTTTCTAGGAACTCATGTTTTGAGGACTCGATTCGTGTTTTTGCAGATATGCTTGACGTTGGGCTGCCATTTGATTCTACAACTTTGGCTGCGGTGCTTACAGCAGTGGCGGAGTTGCAGGAATATAGATTAGGGATGAGTATTCAATGTTTGGCTTCCAAAAAAGGGTTCCATTATGATGTCTACGTGCTTACAGGTTTGATATCATTGTACTCAAAATGTGGGGAGAGTGACAAAGGAAGGTCGTTGTTTGATCAGATTGGTCAGCCAGATTTGATATCTTATAACGCAATGATTTCTGGTTATACTTTCAATCATGAAACTGGGTCGGCAGTGGCACTCTTCAGGGAATTGCTTGCTTCCGGACAGGAAGTTAATTCAAGCACATTGGTGGGCTTAATTCCAGTTTTTTCGCCCTTTGGCCATCTGCAGCTTACTTGCTCTATTCAAACTTTAAGCACGAAGATTGGTATTGTTTCACAACCTTCTGTTTCAACTGCACTCACTACTGTTTACTGTCGACTAAACGAAATTCAATGTGCAAGGCAGCTGTTTGATGAATCTTCGGAGAAAAGTTTGGCTTCCTGGAATGCCATGATATCAGGATACACTCAAAATGGGTTGACAGAGAGAGCAATTTCTCTTTTCCAGGAAATGATGCCTCAACTCAGTCCAAATCCTGTCACCATTACCAGTGTACTTTCGGCTTGCGCTCAGCTTGGAGCTCTAAGTATTGGCAAATGGGTCCATGGATTGATCAAGAGTGAAAAACTTGAATCAAATGTATATGTTGCAACTGCTTTAGTTGATATGCATGCAAAGTGTGGTAGCATTGCAGAGGCTCGACAGTTATTTGACATGATGGCAGAAAAGAATGTGGTAACTTGGAATGCTATGATAACTGGTTATGGTCTTCATGGATATGGAAAAGAAGCACTAAACCTCTTTAATGAGATGTTGCAATCAGGGACTCCACCGACTAGTGTTACTTTTCTATCTATCTTGTATGCTTGCAGTCATTCTGGCTTGGTGAGAGAGGGTAATGAAATTTTCCACTCTATGGTTAACAATTATGGTTTTCAGCCCATGAGCGAGCACTATGCTTGCATGGTCGACATACTTGGGAGAGCTGGACAGCTAAAGAATGCCTTGGAGTTCATTGAAAGAATGCCACTTGAGCCCGGCCCTGCTGTTTGGGGTGCATTGCTTGGTGCCTGCATGATTCACAAGAATACAGACATAGCCCATGTTGCTTCAGAAAGACTTTTCCAATTGGACCCAGAAAATGTAGGATACTATGTCCTGCTTTCAAATATTTATTCTGCTGATAGGAACTTCCCCAAAGCTGCTTCTGTCCGACAAGTTGTTAAGAAGAGAAAACTGGCAAAGACGCCAGGGTGCACTCTTATTGAGATCAGTAATCAACCATATGTTTTCACATCTGGTGATCAGTCCCATCCTCAGGCCACAGCCATTTATGCCATGCTAGAGAAGTTAGCAGGAAAGATGAGAGAGGCTGGATATCAATCGGAGACTGTTACTGCTTTGCATGATGTAGAGGATGAAGAGAAGGAGTTAATGGTGAATGTCCACAGTGAAAAATTAGCAATTGCTTTTGGGCTCATTTCAACTGAACCTGGAACTGAAATTAGGATTATCAAGAACCTCCGAGTTTGTTTAGACTGTCATACTGTAACTAAATTTATATCTAAGATCACAGAGAGAGTGATTGTGGTCAGGGATGCTAATAGGTTCCATCATTTCAAAAATGGTATTTGTTCATGTGGAGACTATTGG

Protein sequence

LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLHAHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFEDSIRVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLISLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSSTLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDESSEKSLASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKWVHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGYGKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYACMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPENVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQATAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPGTEIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW
Homology
BLAST of MS002321 vs. NCBI nr
Match: XP_022135485.1 (pentatricopeptide repeat-containing protein At4g30700 [Momordica charantia] >XP_022135486.1 pentatricopeptide repeat-containing protein At4g30700 [Momordica charantia])

HSP 1 Score: 1406.0 bits (3638), Expect = 0.0e+00
Identity = 701/711 (98.59%), Postives = 705/711 (99.16%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           LFMFNVLIRGFSDNNLPKSSIS+YTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH
Sbjct: 77  LFMFNVLIRGFSDNNLPKSSISVYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 136

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
           AHSIVDGVASNLFVGSATVDLYFKF RADLARKVFDVIPERDTVLWNTMISGFSRNSCFE
Sbjct: 137 AHSIVDGVASNLFVGSATVDLYFKFTRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 196

Query: 121 DSIRVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLI 180
           DSIRVFADMLDVGLPFDSTTLA VLTAV ELQEYRLGMSIQCLASKKGFHYDVYVLTGLI
Sbjct: 197 DSIRVFADMLDVGLPFDSTTLATVLTAVTELQEYRLGMSIQCLASKKGFHYDVYVLTGLI 256

Query: 181 SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS 240
           SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS
Sbjct: 257 SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS 316

Query: 241 TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES 300
           TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES
Sbjct: 317 TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES 376

Query: 301 SEKSLASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW 360
           SEK+LASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW
Sbjct: 377 SEKTLASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW 436

Query: 361 VHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY 420
           VHGLIKSEKLESNVYV+TALVDM+AKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY
Sbjct: 437 VHGLIKSEKLESNVYVSTALVDMYAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY 496

Query: 421 GKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA 480
           GKEALNLFNEMLQSGTPPT VTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA
Sbjct: 497 GKEALNLFNEMLQSGTPPTRVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA 556

Query: 481 CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 540
           CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE
Sbjct: 557 CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 616

Query: 541 NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQ 600
           NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEI NQPYVFTSGDQ HPQ
Sbjct: 617 NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEIGNQPYVFTSGDQCHPQ 676

Query: 601 ATAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPGT 660
           ATAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPGT
Sbjct: 677 ATAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPGT 736

Query: 661 EIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           EIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW
Sbjct: 737 EIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 787

BLAST of MS002321 vs. NCBI nr
Match: XP_023521013.1 (pentatricopeptide repeat-containing protein At4g30700 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 650/712 (91.29%), Postives = 676/712 (94.94%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           LFMFNVLIRGFSDNNLPKSSIS+YTHLRK TNLRPDNFTYAFAISAAS+FEDER+G L+H
Sbjct: 77  LFMFNVLIRGFSDNNLPKSSISVYTHLRKWTNLRPDNFTYAFAISAASKFEDERLGVLMH 136

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
           AHSIVDGVASNLFVGSA VDLYF+F RADLARKVFD +PERDTVLWNTMISGFSRNS FE
Sbjct: 137 AHSIVDGVASNLFVGSAIVDLYFRFTRADLARKVFDAMPERDTVLWNTMISGFSRNSYFE 196

Query: 121 DSIRVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLI 180
           DSIRVF DMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKG H DVYVLTGLI
Sbjct: 197 DSIRVFVDMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGLHSDVYVLTGLI 256

Query: 181 SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS 240
           SL+SKCGESDK R LFDQI QPDLISYNAMISGYTFNHETGSAV LFRELLASGQ V+SS
Sbjct: 257 SLFSKCGESDKARLLFDQIDQPDLISYNAMISGYTFNHETGSAVTLFRELLASGQGVSSS 316

Query: 241 TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES 300
           TLVGLIPVFSPF HLQLT SIQTLS KIGI+S+PSVSTALTTVYCRLNEIQ ARQLFDES
Sbjct: 317 TLVGLIPVFSPFSHLQLTRSIQTLSIKIGIISKPSVSTALTTVYCRLNEIQYARQLFDES 376

Query: 301 SEKSLASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW 360
            EKSLASWNAMISGYTQNGLTE AISLFQEMMPQLSPNPVT+TS+LSACAQLGALS+GKW
Sbjct: 377 PEKSLASWNAMISGYTQNGLTESAISLFQEMMPQLSPNPVTVTSILSACAQLGALSLGKW 436

Query: 361 VHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY 420
           VHGLIKSEKLESN+YV TALVDM+AKCGS+ EARQLFD+ AEKN VTWNAMITGYGLHGY
Sbjct: 437 VHGLIKSEKLESNIYVTTALVDMYAKCGSVVEARQLFDLTAEKNAVTWNAMITGYGLHGY 496

Query: 421 GKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA 480
           G EALNLFN+MLQSG PPT VTFLSILYACSHSGLVREGNEIFHSMVNN+GFQPMSEHYA
Sbjct: 497 GNEALNLFNKMLQSGIPPTGVTFLSILYACSHSGLVREGNEIFHSMVNNFGFQPMSEHYA 556

Query: 481 CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 540
           CMVDI GRAGQL NALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE
Sbjct: 557 CMVDIFGRAGQLTNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 616

Query: 541 NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQ 600
           NVGYYVLLSNIYS DRNFPKAASVRQVVKKR LAKTPGCTLIEI +Q +VFTSGD+SHP+
Sbjct: 617 NVGYYVLLSNIYSTDRNFPKAASVRQVVKKRNLAKTPGCTLIEIDDQQHVFTSGDRSHPR 676

Query: 601 ATAIYAMLEKLAGKMREAGYQSETV-TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 660
           ATAIYAMLEKL GKMREAGYQ+ETV TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG
Sbjct: 677 ATAIYAMLEKLIGKMREAGYQAETVTTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 736

Query: 661 TEIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           TEIRIIKNLRVCLDCHT TKFISKITERVIVVRDANRFHHFK+G+CSCGDYW
Sbjct: 737 TEIRIIKNLRVCLDCHTATKFISKITERVIVVRDANRFHHFKDGLCSCGDYW 788

BLAST of MS002321 vs. NCBI nr
Match: XP_022973439.1 (pentatricopeptide repeat-containing protein At4g30700 [Cucurbita maxima])

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 650/712 (91.29%), Postives = 675/712 (94.80%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           LFMFNVLIRGFSDNNLPKSSIS+YTHLRK TNLRPDNFTYAFAISAAS+FEDER+G LLH
Sbjct: 77  LFMFNVLIRGFSDNNLPKSSISVYTHLRKWTNLRPDNFTYAFAISAASKFEDERLGVLLH 136

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
           AHSIVDGVASNLFVGSA VDLYFKF RAD+ARKVFD +PERDTVLWNTMISGFSRNS FE
Sbjct: 137 AHSIVDGVASNLFVGSAIVDLYFKFTRADMARKVFDAMPERDTVLWNTMISGFSRNSYFE 196

Query: 121 DSIRVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLI 180
           DSIRVF DML VGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKG H DVYVLTGLI
Sbjct: 197 DSIRVFVDMLHVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGLHSDVYVLTGLI 256

Query: 181 SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS 240
           SL+SKCGESDK R LFDQI QPDLISYNAMISGYTFNHETGSAV LFRELLASGQ V+SS
Sbjct: 257 SLFSKCGESDKARLLFDQIDQPDLISYNAMISGYTFNHETGSAVTLFRELLASGQGVSSS 316

Query: 241 TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES 300
           TLVGLIPVFSPF HLQLT SIQTLS KIGI+S+PSVSTALTTVYCRLNEIQ ARQLFDES
Sbjct: 317 TLVGLIPVFSPFSHLQLTRSIQTLSIKIGIISKPSVSTALTTVYCRLNEIQYARQLFDES 376

Query: 301 SEKSLASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW 360
            EKSLASWNAMISGYTQNGLTE AISLFQEM+PQLSPNPVT+TS+LSACAQLGALS+GKW
Sbjct: 377 PEKSLASWNAMISGYTQNGLTESAISLFQEMLPQLSPNPVTVTSILSACAQLGALSLGKW 436

Query: 361 VHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY 420
           VHGLIKSEKLESN+YV TAL+DM+AKCGS+ EARQLFD+MAEKN VTWNAMITGYGLHGY
Sbjct: 437 VHGLIKSEKLESNIYVTTALIDMYAKCGSVVEARQLFDLMAEKNAVTWNAMITGYGLHGY 496

Query: 421 GKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA 480
           G EALNLFN+MLQSG PPT VTFLSILYACSHSGLVREGNEIFHSMVNN+GFQPMSEHYA
Sbjct: 497 GNEALNLFNKMLQSGIPPTGVTFLSILYACSHSGLVREGNEIFHSMVNNFGFQPMSEHYA 556

Query: 481 CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 540
           CMVDI GRAGQL NALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE
Sbjct: 557 CMVDIFGRAGQLTNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 616

Query: 541 NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQ 600
           NVGYYVLLSNIYS DRNFPKAASVRQVVKKR LAKTPGCTLIEI +Q +VFTSGDQSHP+
Sbjct: 617 NVGYYVLLSNIYSTDRNFPKAASVRQVVKKRNLAKTPGCTLIEIDDQQHVFTSGDQSHPR 676

Query: 601 ATAIYAMLEKLAGKMREAGYQSETV-TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 660
           ATAIYAMLEKL GKMREAGYQ+ETV TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG
Sbjct: 677 ATAIYAMLEKLIGKMREAGYQAETVTTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 736

Query: 661 TEIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           TEIRIIKNLRVCLDCHT TKFISKITERVIVVRDANRFHHFK+G CSCGDYW
Sbjct: 737 TEIRIIKNLRVCLDCHTATKFISKITERVIVVRDANRFHHFKDGFCSCGDYW 788

BLAST of MS002321 vs. NCBI nr
Match: XP_022925824.1 (pentatricopeptide repeat-containing protein At4g30700 [Cucurbita moschata])

HSP 1 Score: 1305.4 bits (3377), Expect = 0.0e+00
Identity = 650/712 (91.29%), Postives = 675/712 (94.80%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           LFMFNVLIRGFSDNNLPKSSIS+YTHLRK TNLRPDNFTYAFAISAAS+FEDER+G LLH
Sbjct: 77  LFMFNVLIRGFSDNNLPKSSISVYTHLRKWTNLRPDNFTYAFAISAASKFEDERLGVLLH 136

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
           AHSIVDGVASNLFVGSA VDLYFKF RADLARKVFD +PERDTVLWNTMISGFSRNS FE
Sbjct: 137 AHSIVDGVASNLFVGSAIVDLYFKFTRADLARKVFDAMPERDTVLWNTMISGFSRNSYFE 196

Query: 121 DSIRVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLI 180
           DSIRVF DMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKG H DVYVLTGLI
Sbjct: 197 DSIRVFVDMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGLHSDVYVLTGLI 256

Query: 181 SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS 240
           SL+SKCGESDK R LFDQI QPDLISYNAMISGYTFNHETGSAV LFRELLASGQ V+SS
Sbjct: 257 SLFSKCGESDKARLLFDQIDQPDLISYNAMISGYTFNHETGSAVTLFRELLASGQGVSSS 316

Query: 241 TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES 300
           TLVGLIPVFSPF HLQLT SIQTLS K+GI+S+PSVSTALTTVYCRLNEIQ ARQLFDES
Sbjct: 317 TLVGLIPVFSPFSHLQLTRSIQTLSIKLGIISKPSVSTALTTVYCRLNEIQYARQLFDES 376

Query: 301 SEKSLASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW 360
            EKSLASWNAMISGYTQNGLTE AISLFQEMMPQLSPNPVT+TS+LSACAQLGALS+GKW
Sbjct: 377 PEKSLASWNAMISGYTQNGLTESAISLFQEMMPQLSPNPVTVTSILSACAQLGALSLGKW 436

Query: 361 VHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY 420
           VHGLIKSEKLESN+YV TALVDM+AKCGS+ EARQLFD+ AEKN VTWNAMITGYGLHGY
Sbjct: 437 VHGLIKSEKLESNIYVTTALVDMYAKCGSVVEARQLFDLTAEKNAVTWNAMITGYGLHGY 496

Query: 421 GKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA 480
           G EALNLFN+MLQSG PPT VTFLSILYACSHSGLVREGNEIFHSMVNN+GFQPMSEHYA
Sbjct: 497 GNEALNLFNKMLQSGIPPTGVTFLSILYACSHSGLVREGNEIFHSMVNNFGFQPMSEHYA 556

Query: 481 CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 540
           CMVDI GRAGQL NALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE
Sbjct: 557 CMVDIFGRAGQLTNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 616

Query: 541 NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQ 600
           NVGYYVLLSNIYS DRNFPKAASVRQVVKKR LAKTPGCTLIEI +Q +VFTSGD+SHP+
Sbjct: 617 NVGYYVLLSNIYSTDRNFPKAASVRQVVKKRNLAKTPGCTLIEIDDQQHVFTSGDRSHPR 676

Query: 601 ATAIYAMLEKLAGKMREAGYQSETV-TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 660
           A AIYAMLEKL GKMREAGYQ+ETV TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG
Sbjct: 677 AMAIYAMLEKLIGKMREAGYQAETVTTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 736

Query: 661 TEIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           TEIRIIKNLRVCLDCHT TKFISKITERVIVVRDANRFHHFK+G+CSCGDYW
Sbjct: 737 TEIRIIKNLRVCLDCHTATKFISKITERVIVVRDANRFHHFKDGLCSCGDYW 788

BLAST of MS002321 vs. NCBI nr
Match: XP_038889958.1 (pentatricopeptide repeat-containing protein At4g30700-like isoform X2 [Benincasa hispida] >XP_038889959.1 pentatricopeptide repeat-containing protein At4g30700-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1305.0 bits (3376), Expect = 0.0e+00
Identity = 650/712 (91.29%), Postives = 678/712 (95.22%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           LF+FNVLIRGFSDN+LPKSSI +YTHLRK TNLRPDNFT+AFAISAASRFEDER+G LLH
Sbjct: 77  LFLFNVLIRGFSDNSLPKSSIFLYTHLRKGTNLRPDNFTFAFAISAASRFEDERVGVLLH 136

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
           AHSIVDGVASNLFVGSA VDLYFKF RA+LARKVFDV+PERDTVLWNTMISGFSRNS FE
Sbjct: 137 AHSIVDGVASNLFVGSAIVDLYFKFTRAELARKVFDVMPERDTVLWNTMISGFSRNSYFE 196

Query: 121 DSIRVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLI 180
           DSIRVF DML+ GL FDSTTLAAVLTAVAELQEYRLGM IQCLASKKG H DVYVLTGLI
Sbjct: 197 DSIRVFVDMLNAGLSFDSTTLAAVLTAVAELQEYRLGMGIQCLASKKGLHSDVYVLTGLI 256

Query: 181 SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS 240
           SLYSKCG+SDKGR LFDQI QPDLISYNAMISGYTFNHET SAV LF+ELLASGQ VNSS
Sbjct: 257 SLYSKCGKSDKGRLLFDQIDQPDLISYNAMISGYTFNHETESAVTLFKELLASGQGVNSS 316

Query: 241 TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES 300
           TLVGL+PVFSPF HLQLTC IQ LS KIGI+SQPSVSTALTTVYCRLNE+Q AR+LFDES
Sbjct: 317 TLVGLVPVFSPFNHLQLTCLIQNLSMKIGIISQPSVSTALTTVYCRLNEVQFARKLFDES 376

Query: 301 SEKSLASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW 360
            EKSLASWNAMISGYTQNGLTERAISLFQEM+PQLSPNPVT+TS+LSACAQLGALSIGKW
Sbjct: 377 PEKSLASWNAMISGYTQNGLTERAISLFQEMVPQLSPNPVTVTSILSACAQLGALSIGKW 436

Query: 361 VHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY 420
           VHGLIKSE+LESN+YV+TALVDM+AKCGSI EARQLFD+MAEKNVVTWNAMITGYGLHG+
Sbjct: 437 VHGLIKSERLESNLYVSTALVDMYAKCGSIVEARQLFDLMAEKNVVTWNAMITGYGLHGH 496

Query: 421 GKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA 480
           GKEALNLFNEML+SG P T VTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA
Sbjct: 497 GKEALNLFNEMLRSGIPLTRVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA 556

Query: 481 CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 540
           CMVDILGRAGQL NALEFIERMPLEPGPAVWGALLGACMIHKNT+IAHVAS+RLFQLDPE
Sbjct: 557 CMVDILGRAGQLTNALEFIERMPLEPGPAVWGALLGACMIHKNTEIAHVASKRLFQLDPE 616

Query: 541 NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQ 600
           NVGYYVLLSNIYS DRNFPKAASVRQVVKKRKLAKTPGCTLIEI NQ YVFTSGDQSHPQ
Sbjct: 617 NVGYYVLLSNIYSTDRNFPKAASVRQVVKKRKLAKTPGCTLIEIGNQQYVFTSGDQSHPQ 676

Query: 601 ATAIYAMLEKLAGKMREAGYQSETV-TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 660
           ATAI+AMLEKL GKMREAGYQ+ETV TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG
Sbjct: 677 ATAIFAMLEKLTGKMREAGYQAETVTTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 736

Query: 661 TEIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           TEIRIIKNLRVCLDCHT TKFISKITERVIVVRDANRFHHFKNGICSCGDYW
Sbjct: 737 TEIRIIKNLRVCLDCHTATKFISKITERVIVVRDANRFHHFKNGICSCGDYW 788

BLAST of MS002321 vs. ExPASy Swiss-Prot
Match: Q9SUH6 (Pentatricopeptide repeat-containing protein At4g30700 OS=Arabidopsis thaliana OX=3702 GN=DYW9 PE=2 SV=1)

HSP 1 Score: 961.1 bits (2483), Expect = 7.1e-279
Identity = 474/713 (66.48%), Postives = 574/713 (80.50%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           +F+FNVL+RGFS N  P SS+S++ HLRK T+L+P++ TYAFAISAAS F D+R G ++H
Sbjct: 83  VFLFNVLMRGFSVNESPHSSLSVFAHLRKSTDLKPNSSTYAFAISAASGFRDDRAGRVIH 142

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
             ++VDG  S L +GS  V +YFKF R + ARKVFD +PE+DT+LWNTMISG+ +N  + 
Sbjct: 143 GQAVVDGCDSELLLGSNIVKMYFKFWRVEDARKVFDRMPEKDTILWNTMISGYRKNEMYV 202

Query: 121 DSIRVFADMLDVGLP-FDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGL 180
           +SI+VF D+++      D+TTL  +L AVAELQE RLGM I  LA+K G +   YVLTG 
Sbjct: 203 ESIQVFRDLINESCTRLDTTTLLDILPAVAELQELRLGMQIHSLATKTGCYSHDYVLTGF 262

Query: 181 ISLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNS 240
           ISLYSKCG+   G +LF +  +PD+++YNAMI GYT N ET  +++LF+EL+ SG  + S
Sbjct: 263 ISLYSKCGKIKMGSALFREFRKPDIVAYNAMIHGYTSNGETELSLSLFKELMLSGARLRS 322

Query: 241 STLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDE 300
           STLV L+PV    GHL L  +I     K   +S  SVSTALTTVY +LNEI+ AR+LFDE
Sbjct: 323 STLVSLVPV---SGHLMLIYAIHGYCLKSNFLSHASVSTALTTVYSKLNEIESARKLFDE 382

Query: 301 SSEKSLASWNAMISGYTQNGLTERAISLFQEMM-PQLSPNPVTITSVLSACAQLGALSIG 360
           S EKSL SWNAMISGYTQNGLTE AISLF+EM   + SPNPVTIT +LSACAQLGALS+G
Sbjct: 383 SPEKSLPSWNAMISGYTQNGLTEDAISLFREMQKSEFSPNPVTITCILSACAQLGALSLG 442

Query: 361 KWVHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLH 420
           KWVH L++S   ES++YV+TAL+ M+AKCGSIAEAR+LFD+M +KN VTWN MI+GYGLH
Sbjct: 443 KWVHDLVRSTDFESSIYVSTALIGMYAKCGSIAEARRLFDLMTKKNEVTWNTMISGYGLH 502

Query: 421 GYGKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEH 480
           G G+EALN+F EML SG  PT VTFL +LYACSH+GLV+EG+EIF+SM++ YGF+P  +H
Sbjct: 503 GQGQEALNIFYEMLNSGITPTPVTFLCVLYACSHAGLVKEGDEIFNSMIHRYGFEPSVKH 562

Query: 481 YACMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLD 540
           YACMVDILGRAG L+ AL+FIE M +EPG +VW  LLGAC IHK+T++A   SE+LF+LD
Sbjct: 563 YACMVDILGRAGHLQRALQFIEAMSIEPGSSVWETLLGACRIHKDTNLARTVSEKLFELD 622

Query: 541 PENVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSH 600
           P+NVGY+VLLSNI+SADRN+P+AA+VRQ  KKRKLAK PG TLIEI   P+VFTSGDQSH
Sbjct: 623 PDNVGYHVLLSNIHSADRNYPQAATVRQTAKKRKLAKAPGYTLIEIGETPHVFTSGDQSH 682

Query: 601 PQATAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEP 660
           PQ   IY  LEKL GKMREAGYQ ET  ALHDVE+EE+ELMV VHSE+LAIAFGLI+TEP
Sbjct: 683 PQVKEIYEKLEKLEGKMREAGYQPETELALHDVEEEERELMVKVHSERLAIAFGLIATEP 742

Query: 661 GTEIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           GTEIRIIKNLRVCLDCHTVTK ISKITERVIVVRDANRFHHFK+G+CSCGDYW
Sbjct: 743 GTEIRIIKNLRVCLDCHTVTKLISKITERVIVVRDANRFHHFKDGVCSCGDYW 792

BLAST of MS002321 vs. ExPASy Swiss-Prot
Match: Q3E6Q1 (Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H40 PE=2 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 3.7e-166
Identity = 292/710 (41.13%), Postives = 443/710 (62.39%), Query Frame = 0

Query: 3   MFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLHAH 62
           +++ +++GF+  +    ++  +  +R   ++ P  + + + +       + R+G  +H  
Sbjct: 102 LYHTMLKGFAKVSDLDKALQFFVRMR-YDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGL 161

Query: 63  SIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFEDS 122
            +  G + +LF  +   ++Y K  + + ARKVFD +PERD V WNT+++G+S+N     +
Sbjct: 162 LVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMA 221

Query: 123 IRVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLISL 182
           + +   M +  L     T+ +VL AV+ L+   +G  I   A + GF   V + T L+ +
Sbjct: 222 LEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNISTALVDM 281

Query: 183 YSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSSTL 242
           Y+KCG  +  R LFD + + +++S+N+MI  Y  N     A+ +F+++L  G +    ++
Sbjct: 282 YAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSV 341

Query: 243 VGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDESSE 302
           +G +   +  G L+    I  LS ++G+    SV  +L ++YC+  E+  A  +F +   
Sbjct: 342 MGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMFGKLQS 401

Query: 303 KSLASWNAMISGYTQNGLTERAISLFQEMMPQ-LSPNPVTITSVLSACAQLGALSIGKWV 362
           ++L SWNAMI G+ QNG    A++ F +M  + + P+  T  SV++A A+L      KW+
Sbjct: 402 RTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELSITHHAKWI 461

Query: 363 HGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGYG 422
           HG++    L+ NV+V TALVDM+AKCG+I  AR +FDMM+E++V TWNAMI GYG HG+G
Sbjct: 462 HGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMIDGYGTHGFG 521

Query: 423 KEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYAC 482
           K AL LF EM +    P  VTFLS++ ACSHSGLV  G + F+ M  NY  +   +HY  
Sbjct: 522 KAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKENYSIELSMDHYGA 581

Query: 483 MVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPEN 542
           MVD+LGRAG+L  A +FI +MP++P   V+GA+LGAC IHKN + A  A+ERLF+L+P++
Sbjct: 582 MVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQIHKNVNFAEKAAERLFELNPDD 641

Query: 543 VGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQA 602
            GY+VLL+NIY A   + K   VR  + ++ L KTPGC+++EI N+ + F SG  +HP +
Sbjct: 642 GGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGCSMVEIKNEVHSFFSGSTAHPDS 701

Query: 603 TAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPGTE 662
             IYA LEKL   ++EAGY  +T   L  VE++ KE +++ HSEKLAI+FGL++T  GT 
Sbjct: 702 KKIYAFLEKLICHIKEAGYVPDTNLVL-GVENDVKEQLLSTHSEKLAISFGLLNTTAGTT 761

Query: 663 IRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           I + KNLRVC DCH  TK+IS +T R IVVRD  RFHHFKNG CSCGDYW
Sbjct: 762 IHVRKNLRVCADCHNATKYISLVTGREIVVRDMQRFHHFKNGACSCGDYW 809

BLAST of MS002321 vs. ExPASy Swiss-Prot
Match: O81767 (Pentatricopeptide repeat-containing protein At4g33990 OS=Arabidopsis thaliana OX=3702 GN=EMB2758 PE=3 SV=2)

HSP 1 Score: 537.3 bits (1383), Expect = 2.5e-151
Identity = 280/715 (39.16%), Postives = 426/715 (59.58%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           ++ +N++I G+         I  ++     + L PD  T+   + A     D   G  +H
Sbjct: 117 VYAWNLMISGYGRAGNSSEVIRCFSLFMLSSGLTPDYRTFPSVLKACRTVID---GNKIH 176

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
             ++  G   +++V ++ + LY ++     AR +FD +P RD   WN MISG+ ++   +
Sbjct: 177 CLALKFGFMWDVYVAASLIHLYSRYKAVGNARILFDEMPVRDMGSWNAMISGYCQSGNAK 236

Query: 121 DSIRVFADMLDVGL-PFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGL 180
           +++      L  GL   DS T+ ++L+A  E  ++  G++I   + K G   +++V   L
Sbjct: 237 EAL-----TLSNGLRAMDSVTVVSLLSACTEAGDFNRGVTIHSYSIKHGLESELFVSNKL 296

Query: 181 ISLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNS 240
           I LY++ G     + +FD++   DLIS+N++I  Y  N +   A++LF+E+  S  + + 
Sbjct: 297 IDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDC 356

Query: 241 STLVGLIPVFSPFGHLQLTCSIQTLSTKIG-IVSQPSVSTALTTVYCRLNEIQCARQLFD 300
            TL+ L  + S  G ++   S+Q  + + G  +   ++  A+  +Y +L  +  AR +F+
Sbjct: 357 LTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFN 416

Query: 301 ESSEKSLASWNAMISGYTQNGLTERAISLF--QEMMPQLSPNPVTITSVLSACAQLGALS 360
                 + SWN +ISGY QNG    AI ++   E   +++ N  T  SVL AC+Q GAL 
Sbjct: 417 WLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALR 476

Query: 361 IGKWVHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYG 420
            G  +HG +    L  +V+V T+L DM+ KCG + +A  LF  +   N V WN +I  +G
Sbjct: 477 QGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHG 536

Query: 421 LHGYGKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMS 480
            HG+G++A+ LF EML  G  P  +TF+++L ACSHSGLV EG   F  M  +YG  P  
Sbjct: 537 FHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHSGLVDEGQWCFEMMQTDYGITPSL 596

Query: 481 EHYACMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQ 540
           +HY CMVD+ GRAGQL+ AL+FI+ M L+P  ++WGALL AC +H N D+  +ASE LF+
Sbjct: 597 KHYGCMVDMYGRAGQLETALKFIKSMSLQPDASIWGALLSACRVHGNVDLGKIASEHLFE 656

Query: 541 LDPENVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQ 600
           ++PE+VGY+VLLSN+Y++   +     +R +   + L KTPG + +E+ N+  VF +G+Q
Sbjct: 657 VEPEHVGYHVLLSNMYASAGKWEGVDEIRSIAHGKGLRKTPGWSSMEVDNKVEVFYTGNQ 716

Query: 601 SHPQATAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLIST 660
           +HP    +Y  L  L  K++  GY  +    L DVED+EKE ++  HSE+LAIAF LI+T
Sbjct: 717 THPMYEEMYRELTALQAKLKMIGYVPDHRFVLQDVEDDEKEHILMSHSERLAIAFALIAT 776

Query: 661 EPGTEIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
              T IRI KNLRVC DCH+VTKFISKITER I+VRD+NRFHHFKNG+CSCGDYW
Sbjct: 777 PAKTTIRIFKNLRVCGDCHSVTKFISKITEREIIVRDSNRFHHFKNGVCSCGDYW 823

BLAST of MS002321 vs. ExPASy Swiss-Prot
Match: Q9LTV8 (Pentatricopeptide repeat-containing protein At3g12770 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H43 PE=2 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 9.1e-149
Identity = 266/688 (38.66%), Postives = 420/688 (61.05%), Query Frame = 0

Query: 27  LRKRTNLRPDNFTYAFAISAASRFEDERIGTLLHAHSIVDGVASNLFVGSATVDLYFKFM 86
           L   + +  D+F  +   SA  + + ++I    HA  +V G+  + F+ +  +     F 
Sbjct: 12  LYTNSGIHSDSFYASLIDSATHKAQLKQI----HARLLVLGLQFSGFLITKLIHASSSFG 71

Query: 87  RADLARKVFDVIPERDTVLWNTMISGFSRNSCFEDSIRVFADMLDVGLPFDSTTLAAVLT 146
               AR+VFD +P      WN +I G+SRN+ F+D++ ++++M    +  DS T   +L 
Sbjct: 72  DITFARQVFDDLPRPQIFPWNAIIRGYSRNNHFQDALLMYSNMQLARVSPDSFTFPHLLK 131

Query: 147 AVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLISLYSKCGESDKGRSLFDQIGQPD--L 206
           A + L   ++G  +     + GF  DV+V  GLI+LY+KC      R++F+ +  P+  +
Sbjct: 132 ACSGLSHLQMGRFVHAQVFRLGFDADVFVQNGLIALYAKCRRLGSARTVFEGLPLPERTI 191

Query: 207 ISYNAMISGYTFNHETGSAVALFRELLASGQEVNSSTLVGLIPVFSPFGHLQLTCSIQTL 266
           +S+ A++S Y  N E   A+ +F ++     + +   LV ++  F+    L+   SI   
Sbjct: 192 VSWTAIVSAYAQNGEPMEALEIFSQMRKMDVKPDWVALVSVLNAFTCLQDLKQGRSIHAS 251

Query: 267 STKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDESSEKSLASWNAMISGYTQNGLTERA 326
             K+G+  +P +  +L T+Y +  ++  A+ LFD+    +L  WNAMISGY +NG    A
Sbjct: 252 VVKMGLEIEPDLLISLNTMYAKCGQVATAKILFDKMKSPNLILWNAMISGYAKNGYAREA 311

Query: 327 ISLFQEMM-PQLSPNPVTITSVLSACAQLGALSIGKWVHGLIKSEKLESNVYVATALVDM 386
           I +F EM+   + P+ ++ITS +SACAQ+G+L   + ++  +       +V++++AL+DM
Sbjct: 312 IDMFHEMINKDVRPDTISITSAISACAQVGSLEQARSMYEYVGRSDYRDDVFISSALIDM 371

Query: 387 HAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGYGKEALNLFNEMLQSGTPPTSVTF 446
            AKCGS+  AR +FD   +++VV W+AMI GYGLHG  +EA++L+  M + G  P  VTF
Sbjct: 372 FAKCGSVEGARLVFDRTLDRDVVVWSAMIVGYGLHGRAREAISLYRAMERGGVHPNDVTF 431

Query: 447 LSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYACMVDILGRAGQLKNALEFIERMP 506
           L +L AC+HSG+VREG   F+ M  ++   P  +HYAC++D+LGRAG L  A E I+ MP
Sbjct: 432 LGLLMACNHSGMVREGWWFFNRMA-DHKINPQQQHYACVIDLLGRAGHLDQAYEVIKCMP 491

Query: 507 LEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPENVGYYVLLSNIYSADRNFPKAAS 566
           ++PG  VWGALL AC  H++ ++   A+++LF +DP N G+YV LSN+Y+A R + + A 
Sbjct: 492 VQPGVTVWGALLSACKKHRHVELGEYAAQQLFSIDPSNTGHYVQLSNLYAAARLWDRVAE 551

Query: 567 VRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQATAIYAMLEKLAGKMREAGYQSE 626
           VR  +K++ L K  GC+ +E+  +   F  GD+SHP+   I   +E +  +++E G+ + 
Sbjct: 552 VRVRMKEKGLNKDVGCSWVEVRGRLEAFRVGDKSHPRYEEIERQVEWIESRLKEGGFVAN 611

Query: 627 TVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPGTEIRIIKNLRVCLDCHTVTKFISK 686
              +LHD+ DEE E  +  HSE++AIA+GLIST  GT +RI KNLR C++CH  TK ISK
Sbjct: 612 KDASLHDLNDEEAEETLCSHSERIAIAYGLISTPQGTPLRITKNLRACVNCHAATKLISK 671

Query: 687 ITERVIVVRDANRFHHFKNGICSCGDYW 712
           + +R IVVRD NRFHHFK+G+CSCGDYW
Sbjct: 672 LVDREIVVRDTNRFHHFKDGVCSCGDYW 694

BLAST of MS002321 vs. ExPASy Swiss-Prot
Match: Q9SN39 (Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DOT4 PE=2 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.1e-146
Identity = 272/710 (38.31%), Postives = 407/710 (57.32%), Query Frame = 0

Query: 4   FNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLHAHS 63
           +N+L+   + +     SI ++  +   + +  D++T++    + S       G  LH   
Sbjct: 163 WNILMNELAKSGDFSGSIGLFKKMMS-SGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFI 222

Query: 64  IVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFEDSI 123
           +  G      VG++ V  Y K  R D ARKVFD + ERD + WN++I+G+  N   E  +
Sbjct: 223 LKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGL 282

Query: 124 RVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLISLY 183
            VF  ML  G+  D  T+ +V    A+ +   LG ++  +  K  F  +      L+ +Y
Sbjct: 283 SVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSREDRFCNTLLDMY 342

Query: 184 SKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSSTLV 243
           SKCG+ D  +++F ++    ++SY +MI+GY      G AV LF E+   G   +  T+ 
Sbjct: 343 SKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVT 402

Query: 244 GLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDESSEK 303
            ++   + +  L     +     +  +     VS AL  +Y +   +Q A  +F E   K
Sbjct: 403 AVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVK 462

Query: 304 SLASWNAMISGYTQNGLTERAISLFQEMMPQ--LSPNPVTITSVLSACAQLGALSIGKWV 363
            + SWN +I GY++N     A+SLF  ++ +   SP+  T+  VL ACA L A   G+ +
Sbjct: 463 DIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREI 522

Query: 364 HGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGYG 423
           HG I      S+ +VA +LVDM+AKCG++  A  LFD +A K++V+W  MI GYG+HG+G
Sbjct: 523 HGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFG 582

Query: 424 KEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYAC 483
           KEA+ LFN+M Q+G     ++F+S+LYACSHSGLV EG   F+ M +    +P  EHYAC
Sbjct: 583 KEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEHYAC 642

Query: 484 MVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPEN 543
           +VD+L R G L  A  FIE MP+ P   +WGALL  C IH +  +A   +E++F+L+PEN
Sbjct: 643 IVDMLARTGDLIKAYRFIENMPIPPDATIWGALLCGCRIHHDVKLAEKVAEKVFELEPEN 702

Query: 544 VGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQA 603
            GYYVL++NIY+    + +   +R+ + +R L K PGC+ IEI  +  +F +GD S+P+ 
Sbjct: 703 TGYYVLMANIYAEAEKWEQVKRLRKRIGQRGLRKNPGCSWIEIKGRVNIFVAGDSSNPET 762

Query: 604 TAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPGTE 663
             I A L K+  +M E GY   T  AL D E+ EKE  +  HSEKLA+A G+IS+  G  
Sbjct: 763 ENIEAFLRKVRARMIEEGYSPLTKYALIDAEEMEKEEALCGHSEKLAMALGIISSGHGKI 822

Query: 664 IRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           IR+ KNLRVC DCH + KF+SK+T R IV+RD+NRFH FK+G CSC  +W
Sbjct: 823 IRVTKNLRVCGDCHEMAKFMSKLTRREIVLRDSNRFHQFKDGHCSCRGFW 871

BLAST of MS002321 vs. ExPASy TrEMBL
Match: A0A6J1C0W7 (pentatricopeptide repeat-containing protein At4g30700 OS=Momordica charantia OX=3673 GN=LOC111007429 PE=3 SV=1)

HSP 1 Score: 1406.0 bits (3638), Expect = 0.0e+00
Identity = 701/711 (98.59%), Postives = 705/711 (99.16%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           LFMFNVLIRGFSDNNLPKSSIS+YTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH
Sbjct: 77  LFMFNVLIRGFSDNNLPKSSISVYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 136

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
           AHSIVDGVASNLFVGSATVDLYFKF RADLARKVFDVIPERDTVLWNTMISGFSRNSCFE
Sbjct: 137 AHSIVDGVASNLFVGSATVDLYFKFTRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 196

Query: 121 DSIRVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLI 180
           DSIRVFADMLDVGLPFDSTTLA VLTAV ELQEYRLGMSIQCLASKKGFHYDVYVLTGLI
Sbjct: 197 DSIRVFADMLDVGLPFDSTTLATVLTAVTELQEYRLGMSIQCLASKKGFHYDVYVLTGLI 256

Query: 181 SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS 240
           SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS
Sbjct: 257 SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS 316

Query: 241 TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES 300
           TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES
Sbjct: 317 TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES 376

Query: 301 SEKSLASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW 360
           SEK+LASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW
Sbjct: 377 SEKTLASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW 436

Query: 361 VHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY 420
           VHGLIKSEKLESNVYV+TALVDM+AKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY
Sbjct: 437 VHGLIKSEKLESNVYVSTALVDMYAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY 496

Query: 421 GKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA 480
           GKEALNLFNEMLQSGTPPT VTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA
Sbjct: 497 GKEALNLFNEMLQSGTPPTRVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA 556

Query: 481 CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 540
           CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE
Sbjct: 557 CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 616

Query: 541 NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQ 600
           NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEI NQPYVFTSGDQ HPQ
Sbjct: 617 NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEIGNQPYVFTSGDQCHPQ 676

Query: 601 ATAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPGT 660
           ATAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPGT
Sbjct: 677 ATAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPGT 736

Query: 661 EIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           EIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW
Sbjct: 737 EIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 787

BLAST of MS002321 vs. ExPASy TrEMBL
Match: A0A6J1IEL0 (pentatricopeptide repeat-containing protein At4g30700 OS=Cucurbita maxima OX=3661 GN=LOC111471980 PE=3 SV=1)

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 650/712 (91.29%), Postives = 675/712 (94.80%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           LFMFNVLIRGFSDNNLPKSSIS+YTHLRK TNLRPDNFTYAFAISAAS+FEDER+G LLH
Sbjct: 77  LFMFNVLIRGFSDNNLPKSSISVYTHLRKWTNLRPDNFTYAFAISAASKFEDERLGVLLH 136

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
           AHSIVDGVASNLFVGSA VDLYFKF RAD+ARKVFD +PERDTVLWNTMISGFSRNS FE
Sbjct: 137 AHSIVDGVASNLFVGSAIVDLYFKFTRADMARKVFDAMPERDTVLWNTMISGFSRNSYFE 196

Query: 121 DSIRVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLI 180
           DSIRVF DML VGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKG H DVYVLTGLI
Sbjct: 197 DSIRVFVDMLHVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGLHSDVYVLTGLI 256

Query: 181 SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS 240
           SL+SKCGESDK R LFDQI QPDLISYNAMISGYTFNHETGSAV LFRELLASGQ V+SS
Sbjct: 257 SLFSKCGESDKARLLFDQIDQPDLISYNAMISGYTFNHETGSAVTLFRELLASGQGVSSS 316

Query: 241 TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES 300
           TLVGLIPVFSPF HLQLT SIQTLS KIGI+S+PSVSTALTTVYCRLNEIQ ARQLFDES
Sbjct: 317 TLVGLIPVFSPFSHLQLTRSIQTLSIKIGIISKPSVSTALTTVYCRLNEIQYARQLFDES 376

Query: 301 SEKSLASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW 360
            EKSLASWNAMISGYTQNGLTE AISLFQEM+PQLSPNPVT+TS+LSACAQLGALS+GKW
Sbjct: 377 PEKSLASWNAMISGYTQNGLTESAISLFQEMLPQLSPNPVTVTSILSACAQLGALSLGKW 436

Query: 361 VHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY 420
           VHGLIKSEKLESN+YV TAL+DM+AKCGS+ EARQLFD+MAEKN VTWNAMITGYGLHGY
Sbjct: 437 VHGLIKSEKLESNIYVTTALIDMYAKCGSVVEARQLFDLMAEKNAVTWNAMITGYGLHGY 496

Query: 421 GKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA 480
           G EALNLFN+MLQSG PPT VTFLSILYACSHSGLVREGNEIFHSMVNN+GFQPMSEHYA
Sbjct: 497 GNEALNLFNKMLQSGIPPTGVTFLSILYACSHSGLVREGNEIFHSMVNNFGFQPMSEHYA 556

Query: 481 CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 540
           CMVDI GRAGQL NALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE
Sbjct: 557 CMVDIFGRAGQLTNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 616

Query: 541 NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQ 600
           NVGYYVLLSNIYS DRNFPKAASVRQVVKKR LAKTPGCTLIEI +Q +VFTSGDQSHP+
Sbjct: 617 NVGYYVLLSNIYSTDRNFPKAASVRQVVKKRNLAKTPGCTLIEIDDQQHVFTSGDQSHPR 676

Query: 601 ATAIYAMLEKLAGKMREAGYQSETV-TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 660
           ATAIYAMLEKL GKMREAGYQ+ETV TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG
Sbjct: 677 ATAIYAMLEKLIGKMREAGYQAETVTTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 736

Query: 661 TEIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           TEIRIIKNLRVCLDCHT TKFISKITERVIVVRDANRFHHFK+G CSCGDYW
Sbjct: 737 TEIRIIKNLRVCLDCHTATKFISKITERVIVVRDANRFHHFKDGFCSCGDYW 788

BLAST of MS002321 vs. ExPASy TrEMBL
Match: A0A6J1EDA0 (pentatricopeptide repeat-containing protein At4g30700 OS=Cucurbita moschata OX=3662 GN=LOC111433113 PE=3 SV=1)

HSP 1 Score: 1305.4 bits (3377), Expect = 0.0e+00
Identity = 650/712 (91.29%), Postives = 675/712 (94.80%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           LFMFNVLIRGFSDNNLPKSSIS+YTHLRK TNLRPDNFTYAFAISAAS+FEDER+G LLH
Sbjct: 77  LFMFNVLIRGFSDNNLPKSSISVYTHLRKWTNLRPDNFTYAFAISAASKFEDERLGVLLH 136

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
           AHSIVDGVASNLFVGSA VDLYFKF RADLARKVFD +PERDTVLWNTMISGFSRNS FE
Sbjct: 137 AHSIVDGVASNLFVGSAIVDLYFKFTRADLARKVFDAMPERDTVLWNTMISGFSRNSYFE 196

Query: 121 DSIRVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLI 180
           DSIRVF DMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKG H DVYVLTGLI
Sbjct: 197 DSIRVFVDMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGLHSDVYVLTGLI 256

Query: 181 SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS 240
           SL+SKCGESDK R LFDQI QPDLISYNAMISGYTFNHETGSAV LFRELLASGQ V+SS
Sbjct: 257 SLFSKCGESDKARLLFDQIDQPDLISYNAMISGYTFNHETGSAVTLFRELLASGQGVSSS 316

Query: 241 TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES 300
           TLVGLIPVFSPF HLQLT SIQTLS K+GI+S+PSVSTALTTVYCRLNEIQ ARQLFDES
Sbjct: 317 TLVGLIPVFSPFSHLQLTRSIQTLSIKLGIISKPSVSTALTTVYCRLNEIQYARQLFDES 376

Query: 301 SEKSLASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW 360
            EKSLASWNAMISGYTQNGLTE AISLFQEMMPQLSPNPVT+TS+LSACAQLGALS+GKW
Sbjct: 377 PEKSLASWNAMISGYTQNGLTESAISLFQEMMPQLSPNPVTVTSILSACAQLGALSLGKW 436

Query: 361 VHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY 420
           VHGLIKSEKLESN+YV TALVDM+AKCGS+ EARQLFD+ AEKN VTWNAMITGYGLHGY
Sbjct: 437 VHGLIKSEKLESNIYVTTALVDMYAKCGSVVEARQLFDLTAEKNAVTWNAMITGYGLHGY 496

Query: 421 GKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA 480
           G EALNLFN+MLQSG PPT VTFLSILYACSHSGLVREGNEIFHSMVNN+GFQPMSEHYA
Sbjct: 497 GNEALNLFNKMLQSGIPPTGVTFLSILYACSHSGLVREGNEIFHSMVNNFGFQPMSEHYA 556

Query: 481 CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 540
           CMVDI GRAGQL NALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE
Sbjct: 557 CMVDIFGRAGQLTNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 616

Query: 541 NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQ 600
           NVGYYVLLSNIYS DRNFPKAASVRQVVKKR LAKTPGCTLIEI +Q +VFTSGD+SHP+
Sbjct: 617 NVGYYVLLSNIYSTDRNFPKAASVRQVVKKRNLAKTPGCTLIEIDDQQHVFTSGDRSHPR 676

Query: 601 ATAIYAMLEKLAGKMREAGYQSETV-TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 660
           A AIYAMLEKL GKMREAGYQ+ETV TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG
Sbjct: 677 AMAIYAMLEKLIGKMREAGYQAETVTTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 736

Query: 661 TEIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           TEIRIIKNLRVCLDCHT TKFISKITERVIVVRDANRFHHFK+G+CSCGDYW
Sbjct: 737 TEIRIIKNLRVCLDCHTATKFISKITERVIVVRDANRFHHFKDGLCSCGDYW 788

BLAST of MS002321 vs. ExPASy TrEMBL
Match: A0A5A7U078 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold648G00560 PE=3 SV=1)

HSP 1 Score: 1287.3 bits (3330), Expect = 0.0e+00
Identity = 642/712 (90.17%), Postives = 670/712 (94.10%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           LF+FNVLIRGFSDN LPKSSI +YTHLRKRTNLRPDNFTYAFAISAASR EDER+G LLH
Sbjct: 77  LFLFNVLIRGFSDNGLPKSSIFLYTHLRKRTNLRPDNFTYAFAISAASRLEDERVGVLLH 136

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
           AHSIVDGVASNLFVGSA VDLYFKF RA+LARKVFDV+PERDTVLWNTMISGFSRNS FE
Sbjct: 137 AHSIVDGVASNLFVGSAIVDLYFKFTRAELARKVFDVMPERDTVLWNTMISGFSRNSYFE 196

Query: 121 DSIRVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLI 180
           DSIRVF DMLDVGL FDSTTLA VLTAVAELQEYRLGM IQCLASKKG H DVYVLTGLI
Sbjct: 197 DSIRVFVDMLDVGLSFDSTTLATVLTAVAELQEYRLGMGIQCLASKKGLHSDVYVLTGLI 256

Query: 181 SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS 240
           SLYSKCG+S KGR LFDQI QPDLISYNAMISGYTFNHET SAV LFRELLASGQ VNSS
Sbjct: 257 SLYSKCGKSHKGRILFDQIDQPDLISYNAMISGYTFNHETESAVTLFRELLASGQGVNSS 316

Query: 241 TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES 300
           TLVGLIPV+SPF HLQLT  IQ LS K+GI+ QPSVSTALTTVYCRLNE+Q ARQLFDES
Sbjct: 317 TLVGLIPVYSPFNHLQLTLLIQNLSLKLGIILQPSVSTALTTVYCRLNEVQFARQLFDES 376

Query: 301 SEKSLASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW 360
            EKSLASWNAMISGYTQNGLT+RAISLFQEMMPQLSPNPVT+TS+LSACAQLGALSIGKW
Sbjct: 377 PEKSLASWNAMISGYTQNGLTDRAISLFQEMMPQLSPNPVTVTSILSACAQLGALSIGKW 436

Query: 361 VHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY 420
           VHGLIKSE+LESN+YV+TALVDM+AKCGSI EARQLFD+M +KNVVTWNAMITGYGLHG+
Sbjct: 437 VHGLIKSERLESNLYVSTALVDMYAKCGSIVEARQLFDLMVDKNVVTWNAMITGYGLHGH 496

Query: 421 GKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA 480
           GKEAL LF EMLQSG PPT VTFLSILYACSHSGLVREGNEIFHSM N+YGFQPMSEHYA
Sbjct: 497 GKEALKLFYEMLQSGIPPTGVTFLSILYACSHSGLVREGNEIFHSMANDYGFQPMSEHYA 556

Query: 481 CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 540
           CMVDILGRAGQL NALEFIERMPLEPGPAVWGALLGACMIHKNT+IA+VAS+RLFQLDPE
Sbjct: 557 CMVDILGRAGQLTNALEFIERMPLEPGPAVWGALLGACMIHKNTEIANVASKRLFQLDPE 616

Query: 541 NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQ 600
           NVGYYVLLSNIYS DRNFPKAASVRQVVKKRKLAKTPGCTLIEI +Q YVFTSGD+SHPQ
Sbjct: 617 NVGYYVLLSNIYSTDRNFPKAASVRQVVKKRKLAKTPGCTLIEIGDQQYVFTSGDRSHPQ 676

Query: 601 ATAIYAMLEKLAGKMREAGYQSETV-TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 660
           ATAI+ MLEKL GKMREAGYQ+ETV TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG
Sbjct: 677 ATAIFEMLEKLTGKMREAGYQAETVTTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 736

Query: 661 TEIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           TEIRIIKNLRVCLDCHT TKFISKITERVIVVRDANRFHHFKNGICSCGDYW
Sbjct: 737 TEIRIIKNLRVCLDCHTATKFISKITERVIVVRDANRFHHFKNGICSCGDYW 788

BLAST of MS002321 vs. ExPASy TrEMBL
Match: A0A1S4E1Q1 (pentatricopeptide repeat-containing protein At4g30700 OS=Cucumis melo OX=3656 GN=LOC103492100 PE=3 SV=1)

HSP 1 Score: 1287.3 bits (3330), Expect = 0.0e+00
Identity = 642/712 (90.17%), Postives = 670/712 (94.10%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           LF+FNVLIRGFSDN LPKSSI +YTHLRKRTNLRPDNFTYAFAISAASR EDER+G LLH
Sbjct: 77  LFLFNVLIRGFSDNGLPKSSIFLYTHLRKRTNLRPDNFTYAFAISAASRLEDERVGVLLH 136

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
           AHSIVDGVASNLFVGSA VDLYFKF RA+LARKVFDV+PERDTVLWNTMISGFSRNS FE
Sbjct: 137 AHSIVDGVASNLFVGSAIVDLYFKFTRAELARKVFDVMPERDTVLWNTMISGFSRNSYFE 196

Query: 121 DSIRVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLI 180
           DSIRVF DMLDVGL FDSTTLA VLTAVAELQEYRLGM IQCLASKKG H DVYVLTGLI
Sbjct: 197 DSIRVFVDMLDVGLSFDSTTLATVLTAVAELQEYRLGMGIQCLASKKGLHSDVYVLTGLI 256

Query: 181 SLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSS 240
           SLYSKCG+S KGR LFDQI QPDLISYNAMISGYTFNHET SAV LFRELLASGQ VNSS
Sbjct: 257 SLYSKCGKSHKGRILFDQIDQPDLISYNAMISGYTFNHETESAVTLFRELLASGQGVNSS 316

Query: 241 TLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDES 300
           TLVGLIPV+SPF HLQLT  IQ LS K+GI+ QPSVSTALTTVYCRLNE+Q ARQLFDES
Sbjct: 317 TLVGLIPVYSPFNHLQLTLLIQNLSLKLGIILQPSVSTALTTVYCRLNEVQFARQLFDES 376

Query: 301 SEKSLASWNAMISGYTQNGLTERAISLFQEMMPQLSPNPVTITSVLSACAQLGALSIGKW 360
            EKSLASWNAMISGYTQNGLT+RAISLFQEMMPQLSPNPVT+TS+LSACAQLGALSIGKW
Sbjct: 377 PEKSLASWNAMISGYTQNGLTDRAISLFQEMMPQLSPNPVTVTSILSACAQLGALSIGKW 436

Query: 361 VHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGY 420
           VHGLIKSE+LESN+YV+TALVDM+AKCGSI EARQLFD+M +KNVVTWNAMITGYGLHG+
Sbjct: 437 VHGLIKSERLESNLYVSTALVDMYAKCGSIVEARQLFDLMVDKNVVTWNAMITGYGLHGH 496

Query: 421 GKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYA 480
           GKEAL LF EMLQSG PPT VTFLSILYACSHSGLVREGNEIFHSM N+YGFQPMSEHYA
Sbjct: 497 GKEALKLFYEMLQSGIPPTGVTFLSILYACSHSGLVREGNEIFHSMANDYGFQPMSEHYA 556

Query: 481 CMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPE 540
           CMVDILGRAGQL NALEFIERMPLEPGPAVWGALLGACMIHKNT+IA+VAS+RLFQLDPE
Sbjct: 557 CMVDILGRAGQLTNALEFIERMPLEPGPAVWGALLGACMIHKNTEIANVASKRLFQLDPE 616

Query: 541 NVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQ 600
           NVGYYVLLSNIYS DRNFPKAASVRQVVKKRKLAKTPGCTLIEI +Q YVFTSGD+SHPQ
Sbjct: 617 NVGYYVLLSNIYSTDRNFPKAASVRQVVKKRKLAKTPGCTLIEIGDQQYVFTSGDRSHPQ 676

Query: 601 ATAIYAMLEKLAGKMREAGYQSETV-TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 660
           ATAI+ MLEKL GKMREAGYQ+ETV TALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG
Sbjct: 677 ATAIFEMLEKLTGKMREAGYQAETVTTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPG 736

Query: 661 TEIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           TEIRIIKNLRVCLDCHT TKFISKITERVIVVRDANRFHHFKNGICSCGDYW
Sbjct: 737 TEIRIIKNLRVCLDCHTATKFISKITERVIVVRDANRFHHFKNGICSCGDYW 788

BLAST of MS002321 vs. TAIR 10
Match: AT4G30700.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 961.1 bits (2483), Expect = 5.1e-280
Identity = 474/713 (66.48%), Postives = 574/713 (80.50%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           +F+FNVL+RGFS N  P SS+S++ HLRK T+L+P++ TYAFAISAAS F D+R G ++H
Sbjct: 83  VFLFNVLMRGFSVNESPHSSLSVFAHLRKSTDLKPNSSTYAFAISAASGFRDDRAGRVIH 142

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
             ++VDG  S L +GS  V +YFKF R + ARKVFD +PE+DT+LWNTMISG+ +N  + 
Sbjct: 143 GQAVVDGCDSELLLGSNIVKMYFKFWRVEDARKVFDRMPEKDTILWNTMISGYRKNEMYV 202

Query: 121 DSIRVFADMLDVGLP-FDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGL 180
           +SI+VF D+++      D+TTL  +L AVAELQE RLGM I  LA+K G +   YVLTG 
Sbjct: 203 ESIQVFRDLINESCTRLDTTTLLDILPAVAELQELRLGMQIHSLATKTGCYSHDYVLTGF 262

Query: 181 ISLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNS 240
           ISLYSKCG+   G +LF +  +PD+++YNAMI GYT N ET  +++LF+EL+ SG  + S
Sbjct: 263 ISLYSKCGKIKMGSALFREFRKPDIVAYNAMIHGYTSNGETELSLSLFKELMLSGARLRS 322

Query: 241 STLVGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDE 300
           STLV L+PV    GHL L  +I     K   +S  SVSTALTTVY +LNEI+ AR+LFDE
Sbjct: 323 STLVSLVPV---SGHLMLIYAIHGYCLKSNFLSHASVSTALTTVYSKLNEIESARKLFDE 382

Query: 301 SSEKSLASWNAMISGYTQNGLTERAISLFQEMM-PQLSPNPVTITSVLSACAQLGALSIG 360
           S EKSL SWNAMISGYTQNGLTE AISLF+EM   + SPNPVTIT +LSACAQLGALS+G
Sbjct: 383 SPEKSLPSWNAMISGYTQNGLTEDAISLFREMQKSEFSPNPVTITCILSACAQLGALSLG 442

Query: 361 KWVHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLH 420
           KWVH L++S   ES++YV+TAL+ M+AKCGSIAEAR+LFD+M +KN VTWN MI+GYGLH
Sbjct: 443 KWVHDLVRSTDFESSIYVSTALIGMYAKCGSIAEARRLFDLMTKKNEVTWNTMISGYGLH 502

Query: 421 GYGKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEH 480
           G G+EALN+F EML SG  PT VTFL +LYACSH+GLV+EG+EIF+SM++ YGF+P  +H
Sbjct: 503 GQGQEALNIFYEMLNSGITPTPVTFLCVLYACSHAGLVKEGDEIFNSMIHRYGFEPSVKH 562

Query: 481 YACMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLD 540
           YACMVDILGRAG L+ AL+FIE M +EPG +VW  LLGAC IHK+T++A   SE+LF+LD
Sbjct: 563 YACMVDILGRAGHLQRALQFIEAMSIEPGSSVWETLLGACRIHKDTNLARTVSEKLFELD 622

Query: 541 PENVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSH 600
           P+NVGY+VLLSNI+SADRN+P+AA+VRQ  KKRKLAK PG TLIEI   P+VFTSGDQSH
Sbjct: 623 PDNVGYHVLLSNIHSADRNYPQAATVRQTAKKRKLAKAPGYTLIEIGETPHVFTSGDQSH 682

Query: 601 PQATAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEP 660
           PQ   IY  LEKL GKMREAGYQ ET  ALHDVE+EE+ELMV VHSE+LAIAFGLI+TEP
Sbjct: 683 PQVKEIYEKLEKLEGKMREAGYQPETELALHDVEEEERELMVKVHSERLAIAFGLIATEP 742

Query: 661 GTEIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           GTEIRIIKNLRVCLDCHTVTK ISKITERVIVVRDANRFHHFK+G+CSCGDYW
Sbjct: 743 GTEIRIIKNLRVCLDCHTVTKLISKITERVIVVRDANRFHHFKDGVCSCGDYW 792

BLAST of MS002321 vs. TAIR 10
Match: AT1G11290.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 586.6 bits (1511), Expect = 2.6e-167
Identity = 292/710 (41.13%), Postives = 443/710 (62.39%), Query Frame = 0

Query: 3   MFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLHAH 62
           +++ +++GF+  +    ++  +  +R   ++ P  + + + +       + R+G  +H  
Sbjct: 102 LYHTMLKGFAKVSDLDKALQFFVRMR-YDDVEPVVYNFTYLLKVCGDEAELRVGKEIHGL 161

Query: 63  SIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFEDS 122
            +  G + +LF  +   ++Y K  + + ARKVFD +PERD V WNT+++G+S+N     +
Sbjct: 162 LVKSGFSLDLFAMTGLENMYAKCRQVNEARKVFDRMPERDLVSWNTIVAGYSQNGMARMA 221

Query: 123 IRVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLISL 182
           + +   M +  L     T+ +VL AV+ L+   +G  I   A + GF   V + T L+ +
Sbjct: 222 LEMVKSMCEENLKPSFITIVSVLPAVSALRLISVGKEIHGYAMRSGFDSLVNISTALVDM 281

Query: 183 YSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSSTL 242
           Y+KCG  +  R LFD + + +++S+N+MI  Y  N     A+ +F+++L  G +    ++
Sbjct: 282 YAKCGSLETARQLFDGMLERNVVSWNSMIDAYVQNENPKEAMLIFQKMLDEGVKPTDVSV 341

Query: 243 VGLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDESSE 302
           +G +   +  G L+    I  LS ++G+    SV  +L ++YC+  E+  A  +F +   
Sbjct: 342 MGALHACADLGDLERGRFIHKLSVELGLDRNVSVVNSLISMYCKCKEVDTAASMFGKLQS 401

Query: 303 KSLASWNAMISGYTQNGLTERAISLFQEMMPQ-LSPNPVTITSVLSACAQLGALSIGKWV 362
           ++L SWNAMI G+ QNG    A++ F +M  + + P+  T  SV++A A+L      KW+
Sbjct: 402 RTLVSWNAMILGFAQNGRPIDALNYFSQMRSRTVKPDTFTYVSVITAIAELSITHHAKWI 461

Query: 363 HGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGYG 422
           HG++    L+ NV+V TALVDM+AKCG+I  AR +FDMM+E++V TWNAMI GYG HG+G
Sbjct: 462 HGVVMRSCLDKNVFVTTALVDMYAKCGAIMIARLIFDMMSERHVTTWNAMIDGYGTHGFG 521

Query: 423 KEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYAC 482
           K AL LF EM +    P  VTFLS++ ACSHSGLV  G + F+ M  NY  +   +HY  
Sbjct: 522 KAALELFEEMQKGTIKPNGVTFLSVISACSHSGLVEAGLKCFYMMKENYSIELSMDHYGA 581

Query: 483 MVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPEN 542
           MVD+LGRAG+L  A +FI +MP++P   V+GA+LGAC IHKN + A  A+ERLF+L+P++
Sbjct: 582 MVDLLGRAGRLNEAWDFIMQMPVKPAVNVYGAMLGACQIHKNVNFAEKAAERLFELNPDD 641

Query: 543 VGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQA 602
            GY+VLL+NIY A   + K   VR  + ++ L KTPGC+++EI N+ + F SG  +HP +
Sbjct: 642 GGYHVLLANIYRAASMWEKVGQVRVSMLRQGLRKTPGCSMVEIKNEVHSFFSGSTAHPDS 701

Query: 603 TAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPGTE 662
             IYA LEKL   ++EAGY  +T   L  VE++ KE +++ HSEKLAI+FGL++T  GT 
Sbjct: 702 KKIYAFLEKLICHIKEAGYVPDTNLVL-GVENDVKEQLLSTHSEKLAISFGLLNTTAGTT 761

Query: 663 IRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           I + KNLRVC DCH  TK+IS +T R IVVRD  RFHHFKNG CSCGDYW
Sbjct: 762 IHVRKNLRVCADCHNATKYISLVTGREIVVRDMQRFHHFKNGACSCGDYW 809

BLAST of MS002321 vs. TAIR 10
Match: AT4G33990.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 537.3 bits (1383), Expect = 1.8e-152
Identity = 280/715 (39.16%), Postives = 426/715 (59.58%), Query Frame = 0

Query: 1   LFMFNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLH 60
           ++ +N++I G+         I  ++     + L PD  T+   + A     D   G  +H
Sbjct: 117 VYAWNLMISGYGRAGNSSEVIRCFSLFMLSSGLTPDYRTFPSVLKACRTVID---GNKIH 176

Query: 61  AHSIVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFE 120
             ++  G   +++V ++ + LY ++     AR +FD +P RD   WN MISG+ ++   +
Sbjct: 177 CLALKFGFMWDVYVAASLIHLYSRYKAVGNARILFDEMPVRDMGSWNAMISGYCQSGNAK 236

Query: 121 DSIRVFADMLDVGL-PFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGL 180
           +++      L  GL   DS T+ ++L+A  E  ++  G++I   + K G   +++V   L
Sbjct: 237 EAL-----TLSNGLRAMDSVTVVSLLSACTEAGDFNRGVTIHSYSIKHGLESELFVSNKL 296

Query: 181 ISLYSKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNS 240
           I LY++ G     + +FD++   DLIS+N++I  Y  N +   A++LF+E+  S  + + 
Sbjct: 297 IDLYAEFGRLRDCQKVFDRMYVRDLISWNSIIKAYELNEQPLRAISLFQEMRLSRIQPDC 356

Query: 241 STLVGLIPVFSPFGHLQLTCSIQTLSTKIG-IVSQPSVSTALTTVYCRLNEIQCARQLFD 300
            TL+ L  + S  G ++   S+Q  + + G  +   ++  A+  +Y +L  +  AR +F+
Sbjct: 357 LTLISLASILSQLGDIRACRSVQGFTLRKGWFLEDITIGNAVVVMYAKLGLVDSARAVFN 416

Query: 301 ESSEKSLASWNAMISGYTQNGLTERAISLF--QEMMPQLSPNPVTITSVLSACAQLGALS 360
                 + SWN +ISGY QNG    AI ++   E   +++ N  T  SVL AC+Q GAL 
Sbjct: 417 WLPNTDVISWNTIISGYAQNGFASEAIEMYNIMEEEGEIAANQGTWVSVLPACSQAGALR 476

Query: 361 IGKWVHGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYG 420
            G  +HG +    L  +V+V T+L DM+ KCG + +A  LF  +   N V WN +I  +G
Sbjct: 477 QGMKLHGRLLKNGLYLDVFVVTSLADMYGKCGRLEDALSLFYQIPRVNSVPWNTLIACHG 536

Query: 421 LHGYGKEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMS 480
            HG+G++A+ LF EML  G  P  +TF+++L ACSHSGLV EG   F  M  +YG  P  
Sbjct: 537 FHGHGEKAVMLFKEMLDEGVKPDHITFVTLLSACSHSGLVDEGQWCFEMMQTDYGITPSL 596

Query: 481 EHYACMVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQ 540
           +HY CMVD+ GRAGQL+ AL+FI+ M L+P  ++WGALL AC +H N D+  +ASE LF+
Sbjct: 597 KHYGCMVDMYGRAGQLETALKFIKSMSLQPDASIWGALLSACRVHGNVDLGKIASEHLFE 656

Query: 541 LDPENVGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQ 600
           ++PE+VGY+VLLSN+Y++   +     +R +   + L KTPG + +E+ N+  VF +G+Q
Sbjct: 657 VEPEHVGYHVLLSNMYASAGKWEGVDEIRSIAHGKGLRKTPGWSSMEVDNKVEVFYTGNQ 716

Query: 601 SHPQATAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLIST 660
           +HP    +Y  L  L  K++  GY  +    L DVED+EKE ++  HSE+LAIAF LI+T
Sbjct: 717 THPMYEEMYRELTALQAKLKMIGYVPDHRFVLQDVEDDEKEHILMSHSERLAIAFALIAT 776

Query: 661 EPGTEIRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
              T IRI KNLRVC DCH+VTKFISKITER I+VRD+NRFHHFKNG+CSCGDYW
Sbjct: 777 PAKTTIRIFKNLRVCGDCHSVTKFISKITEREIIVRDSNRFHHFKNGVCSCGDYW 823

BLAST of MS002321 vs. TAIR 10
Match: AT3G12770.1 (mitochondrial editing factor 22 )

HSP 1 Score: 528.9 bits (1361), Expect = 6.4e-150
Identity = 266/688 (38.66%), Postives = 420/688 (61.05%), Query Frame = 0

Query: 27  LRKRTNLRPDNFTYAFAISAASRFEDERIGTLLHAHSIVDGVASNLFVGSATVDLYFKFM 86
           L   + +  D+F  +   SA  + + ++I    HA  +V G+  + F+ +  +     F 
Sbjct: 12  LYTNSGIHSDSFYASLIDSATHKAQLKQI----HARLLVLGLQFSGFLITKLIHASSSFG 71

Query: 87  RADLARKVFDVIPERDTVLWNTMISGFSRNSCFEDSIRVFADMLDVGLPFDSTTLAAVLT 146
               AR+VFD +P      WN +I G+SRN+ F+D++ ++++M    +  DS T   +L 
Sbjct: 72  DITFARQVFDDLPRPQIFPWNAIIRGYSRNNHFQDALLMYSNMQLARVSPDSFTFPHLLK 131

Query: 147 AVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLISLYSKCGESDKGRSLFDQIGQPD--L 206
           A + L   ++G  +     + GF  DV+V  GLI+LY+KC      R++F+ +  P+  +
Sbjct: 132 ACSGLSHLQMGRFVHAQVFRLGFDADVFVQNGLIALYAKCRRLGSARTVFEGLPLPERTI 191

Query: 207 ISYNAMISGYTFNHETGSAVALFRELLASGQEVNSSTLVGLIPVFSPFGHLQLTCSIQTL 266
           +S+ A++S Y  N E   A+ +F ++     + +   LV ++  F+    L+   SI   
Sbjct: 192 VSWTAIVSAYAQNGEPMEALEIFSQMRKMDVKPDWVALVSVLNAFTCLQDLKQGRSIHAS 251

Query: 267 STKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDESSEKSLASWNAMISGYTQNGLTERA 326
             K+G+  +P +  +L T+Y +  ++  A+ LFD+    +L  WNAMISGY +NG    A
Sbjct: 252 VVKMGLEIEPDLLISLNTMYAKCGQVATAKILFDKMKSPNLILWNAMISGYAKNGYAREA 311

Query: 327 ISLFQEMM-PQLSPNPVTITSVLSACAQLGALSIGKWVHGLIKSEKLESNVYVATALVDM 386
           I +F EM+   + P+ ++ITS +SACAQ+G+L   + ++  +       +V++++AL+DM
Sbjct: 312 IDMFHEMINKDVRPDTISITSAISACAQVGSLEQARSMYEYVGRSDYRDDVFISSALIDM 371

Query: 387 HAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGYGKEALNLFNEMLQSGTPPTSVTF 446
            AKCGS+  AR +FD   +++VV W+AMI GYGLHG  +EA++L+  M + G  P  VTF
Sbjct: 372 FAKCGSVEGARLVFDRTLDRDVVVWSAMIVGYGLHGRAREAISLYRAMERGGVHPNDVTF 431

Query: 447 LSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYACMVDILGRAGQLKNALEFIERMP 506
           L +L AC+HSG+VREG   F+ M  ++   P  +HYAC++D+LGRAG L  A E I+ MP
Sbjct: 432 LGLLMACNHSGMVREGWWFFNRMA-DHKINPQQQHYACVIDLLGRAGHLDQAYEVIKCMP 491

Query: 507 LEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPENVGYYVLLSNIYSADRNFPKAAS 566
           ++PG  VWGALL AC  H++ ++   A+++LF +DP N G+YV LSN+Y+A R + + A 
Sbjct: 492 VQPGVTVWGALLSACKKHRHVELGEYAAQQLFSIDPSNTGHYVQLSNLYAAARLWDRVAE 551

Query: 567 VRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQATAIYAMLEKLAGKMREAGYQSE 626
           VR  +K++ L K  GC+ +E+  +   F  GD+SHP+   I   +E +  +++E G+ + 
Sbjct: 552 VRVRMKEKGLNKDVGCSWVEVRGRLEAFRVGDKSHPRYEEIERQVEWIESRLKEGGFVAN 611

Query: 627 TVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPGTEIRIIKNLRVCLDCHTVTKFISK 686
              +LHD+ DEE E  +  HSE++AIA+GLIST  GT +RI KNLR C++CH  TK ISK
Sbjct: 612 KDASLHDLNDEEAEETLCSHSERIAIAYGLISTPQGTPLRITKNLRACVNCHAATKLISK 671

Query: 687 ITERVIVVRDANRFHHFKNGICSCGDYW 712
           + +R IVVRD NRFHHFK+G+CSCGDYW
Sbjct: 672 LVDREIVVRDTNRFHHFKDGVCSCGDYW 694

BLAST of MS002321 vs. TAIR 10
Match: AT4G18750.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 521.9 bits (1343), Expect = 7.9e-148
Identity = 272/710 (38.31%), Postives = 407/710 (57.32%), Query Frame = 0

Query: 4   FNVLIRGFSDNNLPKSSISIYTHLRKRTNLRPDNFTYAFAISAASRFEDERIGTLLHAHS 63
           +N+L+   + +     SI ++  +   + +  D++T++    + S       G  LH   
Sbjct: 163 WNILMNELAKSGDFSGSIGLFKKMMS-SGVEMDSYTFSCVSKSFSSLRSVHGGEQLHGFI 222

Query: 64  IVDGVASNLFVGSATVDLYFKFMRADLARKVFDVIPERDTVLWNTMISGFSRNSCFEDSI 123
           +  G      VG++ V  Y K  R D ARKVFD + ERD + WN++I+G+  N   E  +
Sbjct: 223 LKSGFGERNSVGNSLVAFYLKNQRVDSARKVFDEMTERDVISWNSIINGYVSNGLAEKGL 282

Query: 124 RVFADMLDVGLPFDSTTLAAVLTAVAELQEYRLGMSIQCLASKKGFHYDVYVLTGLISLY 183
            VF  ML  G+  D  T+ +V    A+ +   LG ++  +  K  F  +      L+ +Y
Sbjct: 283 SVFVQMLVSGIEIDLATIVSVFAGCADSRLISLGRAVHSIGVKACFSREDRFCNTLLDMY 342

Query: 184 SKCGESDKGRSLFDQIGQPDLISYNAMISGYTFNHETGSAVALFRELLASGQEVNSSTLV 243
           SKCG+ D  +++F ++    ++SY +MI+GY      G AV LF E+   G   +  T+ 
Sbjct: 343 SKCGDLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVT 402

Query: 244 GLIPVFSPFGHLQLTCSIQTLSTKIGIVSQPSVSTALTTVYCRLNEIQCARQLFDESSEK 303
            ++   + +  L     +     +  +     VS AL  +Y +   +Q A  +F E   K
Sbjct: 403 AVLNCCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVK 462

Query: 304 SLASWNAMISGYTQNGLTERAISLFQEMMPQ--LSPNPVTITSVLSACAQLGALSIGKWV 363
            + SWN +I GY++N     A+SLF  ++ +   SP+  T+  VL ACA L A   G+ +
Sbjct: 463 DIISWNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREI 522

Query: 364 HGLIKSEKLESNVYVATALVDMHAKCGSIAEARQLFDMMAEKNVVTWNAMITGYGLHGYG 423
           HG I      S+ +VA +LVDM+AKCG++  A  LFD +A K++V+W  MI GYG+HG+G
Sbjct: 523 HGYIMRNGYFSDRHVANSLVDMYAKCGALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFG 582

Query: 424 KEALNLFNEMLQSGTPPTSVTFLSILYACSHSGLVREGNEIFHSMVNNYGFQPMSEHYAC 483
           KEA+ LFN+M Q+G     ++F+S+LYACSHSGLV EG   F+ M +    +P  EHYAC
Sbjct: 583 KEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTVEHYAC 642

Query: 484 MVDILGRAGQLKNALEFIERMPLEPGPAVWGALLGACMIHKNTDIAHVASERLFQLDPEN 543
           +VD+L R G L  A  FIE MP+ P   +WGALL  C IH +  +A   +E++F+L+PEN
Sbjct: 643 IVDMLARTGDLIKAYRFIENMPIPPDATIWGALLCGCRIHHDVKLAEKVAEKVFELEPEN 702

Query: 544 VGYYVLLSNIYSADRNFPKAASVRQVVKKRKLAKTPGCTLIEISNQPYVFTSGDQSHPQA 603
            GYYVL++NIY+    + +   +R+ + +R L K PGC+ IEI  +  +F +GD S+P+ 
Sbjct: 703 TGYYVLMANIYAEAEKWEQVKRLRKRIGQRGLRKNPGCSWIEIKGRVNIFVAGDSSNPET 762

Query: 604 TAIYAMLEKLAGKMREAGYQSETVTALHDVEDEEKELMVNVHSEKLAIAFGLISTEPGTE 663
             I A L K+  +M E GY   T  AL D E+ EKE  +  HSEKLA+A G+IS+  G  
Sbjct: 763 ENIEAFLRKVRARMIEEGYSPLTKYALIDAEEMEKEEALCGHSEKLAMALGIISSGHGKI 822

Query: 664 IRIIKNLRVCLDCHTVTKFISKITERVIVVRDANRFHHFKNGICSCGDYW 712
           IR+ KNLRVC DCH + KF+SK+T R IV+RD+NRFH FK+G CSC  +W
Sbjct: 823 IRVTKNLRVCGDCHEMAKFMSKLTRREIVLRDSNRFHQFKDGHCSCRGFW 871

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135485.10.0e+0098.59pentatricopeptide repeat-containing protein At4g30700 [Momordica charantia] >XP_... [more]
XP_023521013.10.0e+0091.29pentatricopeptide repeat-containing protein At4g30700 [Cucurbita pepo subsp. pep... [more]
XP_022973439.10.0e+0091.29pentatricopeptide repeat-containing protein At4g30700 [Cucurbita maxima][more]
XP_022925824.10.0e+0091.29pentatricopeptide repeat-containing protein At4g30700 [Cucurbita moschata][more]
XP_038889958.10.0e+0091.29pentatricopeptide repeat-containing protein At4g30700-like isoform X2 [Benincasa... [more]
Match NameE-valueIdentityDescription
Q9SUH67.1e-27966.48Pentatricopeptide repeat-containing protein At4g30700 OS=Arabidopsis thaliana OX... [more]
Q3E6Q13.7e-16641.13Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidop... [more]
O817672.5e-15139.16Pentatricopeptide repeat-containing protein At4g33990 OS=Arabidopsis thaliana OX... [more]
Q9LTV89.1e-14938.66Pentatricopeptide repeat-containing protein At3g12770 OS=Arabidopsis thaliana OX... [more]
Q9SN391.1e-14638.31Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis t... [more]
Match NameE-valueIdentityDescription
A0A6J1C0W70.0e+0098.59pentatricopeptide repeat-containing protein At4g30700 OS=Momordica charantia OX=... [more]
A0A6J1IEL00.0e+0091.29pentatricopeptide repeat-containing protein At4g30700 OS=Cucurbita maxima OX=366... [more]
A0A6J1EDA00.0e+0091.29pentatricopeptide repeat-containing protein At4g30700 OS=Cucurbita moschata OX=3... [more]
A0A5A7U0780.0e+0090.17Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S4E1Q10.0e+0090.17pentatricopeptide repeat-containing protein At4g30700 OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT4G30700.15.1e-28066.48Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G11290.12.6e-16741.13Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G33990.11.8e-15239.16Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G12770.16.4e-15038.66mitochondrial editing factor 22 [more]
AT4G18750.17.9e-14838.31Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 2..160
e-value: 6.7E-21
score: 77.0
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 357..471
e-value: 5.6E-28
score: 100.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 161..256
e-value: 1.4E-14
score: 55.8
coord: 257..356
e-value: 7.7E-16
score: 59.9
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 278..564
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 104..137
e-value: 2.6E-6
score: 25.3
coord: 406..439
e-value: 2.7E-7
score: 28.4
coord: 307..332
e-value: 1.5E-6
score: 26.0
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 177..198
e-value: 0.16
score: 12.3
coord: 205..234
e-value: 0.0035
score: 17.5
coord: 479..502
e-value: 0.17
score: 12.2
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 306..350
e-value: 8.7E-9
score: 35.5
coord: 403..450
e-value: 9.5E-13
score: 48.1
coord: 102..148
e-value: 2.8E-7
score: 30.6
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 102..136
score: 10.775016
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 304..339
score: 10.073492
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 203..237
score: 9.251395
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 404..438
score: 12.627475
IPR032867DYW domainPFAMPF14432DYW_deaminasecoord: 577..701
e-value: 3.2E-42
score: 143.4
NoneNo IPR availablePANTHERPTHR47924PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 1..705
NoneNo IPR availablePANTHERPTHR47924:SF42PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 1..705

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002321.1MS002321.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding