MS002290 (gene) Bitter gourd (TR) v1

Overview
NameMS002290
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMannosyltransferase
Locationscaffold30: 3873400 .. 3876500 (-)
RNA-Seq ExpressionMS002290
SyntenyMS002290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTCAGAAGAGACCCAGGTCTTCAGCTGCAGAATCAAAATCAGACAGAATCGAAGACTCACCAGAATTTCTTGGTTCCGAGAATCCCAATTCTCAAAAGCATGGTCTTTTCTCTTCGCCGAACAAGGTGTTTGTGTTCTGCTTGGCATTTCGAATTGCAAATGCGCTTTTATTGCAGACTTATTTCAACCCAGATGAACACTGGCAAGCCCTAGAAGTTGCCCACGGCATCACCTTTGGGTAACCTTTCTCTTGGTCCAATCGCTTCCCTGTTATTTTTCTCCTTGCCATTTTCATTGCTATTTCTCTCTGTTCTTACTCTTTCCATTTCTTTTTCCTGGTTTCATGGCTATAATTTATTGATGAATTAGCTTTTGGAAGCTGTAGAACACTTCATGTAATTCAATTGTGGGTTTCCATTTCGCAGGTATGGCCATCTGACGTGGGAATGGAAGAGGGGAATTCGTAGCTACTTGCATCCTTTGTTGTTTGCTTTTCTTTACAAAGTTCTGGCTCTACTCGGCCTTGATACTCCATTGTTCATGGTAAAATGTTAAACTGCATACTTAAACTACTGAAACTTTTATATTGCCTCTGGCAAATTGAATTGTTAAATCCTTGACTATTATATTTGATCAGGTGGGCTTAGATTTTGAGAGATCGACTTTATTCAATTCCATAACACATCTCCAGAAATTAGTTTTACATTGTTGCAAGTGAAATGACATCGATCTTTTGATGGAGTTTCTTTGTTATTGGCCTTTCCCTTAATTTGATTTTATACTCTCTTCACTATGCTTCATAGATGAAAGCTCCGAGGCTGATGCAGTCCATATTCTCAGCTATTGGTGATTTTTACTTGTACAAACTCTCCAGTGTCCTCTTTGGGGATTACGTTGCAAAATGGGCAGTATCCTTCTGTTGGCGATTGGCATGTATATAATCTTCAGTTTCTCTCTTTCCATCTTGGAACTTTCATCCAATCATATCTTCTTAATGACAAAAGGAAAGAAATGTGTTGTGGTTTTTAAACATGATATACTTAACTCTATTCCAGCTGTTTTCCCAACTCACAAACTGGTTTATGTTCTTCTGCTTCAATCGAACTCTATCAAACAGCTTAGAGACTGTTCTTACACTTGTCAGCTTGTATTATTGGCCTTGTATAAGAATTGCTCCCACCAAATTTTCCAAGGTTTCAAGGAAGTTGGCTTTGTTTATGGCAGCATTATCATGTGCTGTTCGCCCAACAAGTGCGATTACTTGGCTCTATGTTGGTCTCCTCGAGCTGTGTACGGCACATGATCGACTAAGATTTATCTTCTTAGAGGCTGCTCCTATTGGGTATGTGTCCCATATCCTCCTATATGTTATAGCATGTTTTACTTATTGTTCTTGGTTTCTTGAATAGGTTTGGCTTTTTCCCCATCTTTATGATAGTTTTATTTGAACTTCATTTTTTTAATTAGTGATGTAAAAACCAATATTACAGAGATATTATTTAACAATAGGATTGAATTTCTTATTAATTATGAGATTGTGGTTTAGAGGATGCATTTTTTGCGAGATTGTAAGCATACAGATTATATGAAGAGTCGGTTCCTAATTTAATATGAATGGTGTATTGCGATTTTCTTTTATGGGCTGCCCCTTTGTTTATCTTTTTCCTCATTTGATAAAAAGATCATTAGTCCCCATTGGTTATCTGGATAGACCGTGTCTTATTTTGGGTCAAATAGTTAAAATTGCACCCCGTGGTATATTGAGGAGATTCTCGCACTTATTTTATTAACCTCTCTAAGATTTCATAGTGTTTCCATCTCTCTTATCCCTCTAACTTGTTATTTCAGGACGTTGGTGCTTGGGATTACATGTTTGTTGGATAGATTAATGTATGGCTTCTGGATCTTAGTACCTCTGAATTTTCTAAAGTTCAATGTTCTCTCCTCTGGTGGAGACTTTTATGGAACTCACAAGTGGCACTGGTACTTCACCCAGGGTTTTACTGCCATGCTCTTCAGTTTCGTTCCATTTTCCATTTCTGGCATCATCACCTCGAGAAATTGGAAACTTACAGGTCTTATCATCTGGGTTCTGGGACTTCACAGTCTACTAGGTCATAAAGAATTCAGGTATCTCTTTCAAGGGAAGCTTGTTTCGCTCAATGAAAATCCCGACCTAAATCGGTTTGGCATTAGTTTGAAGTAATGTGTTTGTTATTTACTATGGTACAGGTTTGTTCTGTCCATTCTTCCAATAACATTGATGTTCTCTGGCTATTCATTAGCCGCCCTCAGATATCCCGCTTCTCCAAATGGTAGAAGGAAAAAAACGGGAGACGTCCACTCCAAACGCCCTATCAAAATTAAGTTGGCCATAATATTTTTGCTAGCTACAAATATCCCAATGGGTTTATACATGAGTTTGGTTCATCAGGTATCTATATTGGCCAATCAGTAAAACAAATATACAAAGTTGGCTGCCATTTTGTTTCTTCTGTTTGATATCTAACCAGCAGACATGGTGTGCAGAGAGGAACAGAGGATGTCATGAATCATCTGGCTCGGAAAGCTGTGAATGAGAAAGTCAAAAGCATCCTCTTTTTGATGCCCTGTCATTCAACGCCTTACTATTCCACTCTGCACCAAAATTTACCAATGCGGTTTCTGGACTGCACACCAAGGTGCGTAAACTCGACAAATTACAGTACTCTTTCCCTTGTACTTCTAGTTATTTTTGTTCATTTGTGATGCTGGCTATTTATCTTACAGCGAAGAGAAAGAAATCCCAGTCGAGTCAGACCGTTTCTTGACGAACCCGCTTGATTTTGCAACGGAATTTGCTAAAAATTGGTCTGTCCCAAGTCACATTGTACTGTTTGATTCAGAAGAGAGACTACTCAAGGACTTTCTGATGTCACATTCTTTCAAGGAGGTTCGATGTGTTGTATTGTATTTGCTAATCATTAATCATCATGGTTCCTGTTGTCGGAAACTAAACTTTGTGTTGTGTGGAATACAGGATAGGAGATTCTTCCACGCTCACTTCAAGGTGGACCGCGATCTTCAATCTTCGGTGGTTTTGTATGTCTTGGCCAAC

mRNA sequence

ATGATTCAGAAGAGACCCAGGTCTTCAGCTGCAGAATCAAAATCAGACAGAATCGAAGACTCACCAGAATTTCTTGGTTCCGAGAATCCCAATTCTCAAAAGCATGGTCTTTTCTCTTCGCCGAACAAGGTGTTTGTGTTCTGCTTGGCATTTCGAATTGCAAATGCGCTTTTATTGCAGACTTATTTCAACCCAGATGAACACTGGCAAGCCCTAGAAGTTGCCCACGGCATCACCTTTGGGTATGGCCATCTGACGTGGGAATGGAAGAGGGGAATTCGTAGCTACTTGCATCCTTTGTTGTTTGCTTTTCTTTACAAAGTTCTGGCTCTACTCGGCCTTGATACTCCATTGTTCATGATGAAAGCTCCGAGGCTGATGCAGTCCATATTCTCAGCTATTGGTGATTTTTACTTGTACAAACTCTCCAGTGTCCTCTTTGGGGATTACGTTGCAAAATGGGCACTGTTTTCCCAACTCACAAACTGGTTTATGTTCTTCTGCTTCAATCGAACTCTATCAAACAGCTTAGAGACTGTTCTTACACTTGTCAGCTTGTATTATTGGCCTTGTATAAGAATTGCTCCCACCAAATTTTCCAAGGTTTCAAGGAAGTTGGCTTTGTTTATGGCAGCATTATCATGTGCTGTTCGCCCAACAAGTGCGATTACTTGGCTCTATGTTGGTCTCCTCGAGCTGTGTACGGCACATGATCGACTAAGATTTATCTTCTTAGAGGCTGCTCCTATTGGGACGTTGGTGCTTGGGATTACATGTTTGTTGGATAGATTAATGTATGGCTTCTGGATCTTAGTACCTCTGAATTTTCTAAAGTTCAATGTTCTCTCCTCTGGTGGAGACTTTTATGGAACTCACAAGTGGCACTGGTACTTCACCCAGGGTTTTACTGCCATGCTCTTCAGTTTCGTTCCATTTTCCATTTCTGGCATCATCACCTCGAGAAATTGGAAACTTACAGGTCTTATCATCTGGGTTCTGGGACTTCACAGTCTACTAGGTCATAAAGAATTCAGGTTTGTTCTGTCCATTCTTCCAATAACATTGATGTTCTCTGGCTATTCATTAGCCGCCCTCAGATATCCCGCTTCTCCAAATGGTAGAAGGAAAAAAACGGGAGACGTCCACTCCAAACGCCCTATCAAAATTAAGTTGGCCATAATATTTTTGCTAGCTACAAATATCCCAATGGGTTTATACATGAGTTTGGTTCATCAGAGAGGAACAGAGGATGTCATGAATCATCTGGCTCGGAAAGCTGTGAATGAGAAAGTCAAAAGCATCCTCTTTTTGATGCCCTGTCATTCAACGCCTTACTATTCCACTCTGCACCAAAATTTACCAATGCGGTTTCTGGACTGCACACCAAGCGAAGAGAAAGAAATCCCAGTCGAGTCAGACCGTTTCTTGACGAACCCGCTTGATTTTGCAACGGAATTTGCTAAAAATTGGTCTGTCCCAAGTCACATTGTACTGTTTGATTCAGAAGAGAGACTACTCAAGGACTTTCTGATGTCACATTCTTTCAAGGAGGATAGGAGATTCTTCCACGCTCACTTCAAGGTGGACCGCGATCTTCAATCTTCGGTGGTTTTGTATGTCTTGGCCAAC

Coding sequence (CDS)

ATGATTCAGAAGAGACCCAGGTCTTCAGCTGCAGAATCAAAATCAGACAGAATCGAAGACTCACCAGAATTTCTTGGTTCCGAGAATCCCAATTCTCAAAAGCATGGTCTTTTCTCTTCGCCGAACAAGGTGTTTGTGTTCTGCTTGGCATTTCGAATTGCAAATGCGCTTTTATTGCAGACTTATTTCAACCCAGATGAACACTGGCAAGCCCTAGAAGTTGCCCACGGCATCACCTTTGGGTATGGCCATCTGACGTGGGAATGGAAGAGGGGAATTCGTAGCTACTTGCATCCTTTGTTGTTTGCTTTTCTTTACAAAGTTCTGGCTCTACTCGGCCTTGATACTCCATTGTTCATGATGAAAGCTCCGAGGCTGATGCAGTCCATATTCTCAGCTATTGGTGATTTTTACTTGTACAAACTCTCCAGTGTCCTCTTTGGGGATTACGTTGCAAAATGGGCACTGTTTTCCCAACTCACAAACTGGTTTATGTTCTTCTGCTTCAATCGAACTCTATCAAACAGCTTAGAGACTGTTCTTACACTTGTCAGCTTGTATTATTGGCCTTGTATAAGAATTGCTCCCACCAAATTTTCCAAGGTTTCAAGGAAGTTGGCTTTGTTTATGGCAGCATTATCATGTGCTGTTCGCCCAACAAGTGCGATTACTTGGCTCTATGTTGGTCTCCTCGAGCTGTGTACGGCACATGATCGACTAAGATTTATCTTCTTAGAGGCTGCTCCTATTGGGACGTTGGTGCTTGGGATTACATGTTTGTTGGATAGATTAATGTATGGCTTCTGGATCTTAGTACCTCTGAATTTTCTAAAGTTCAATGTTCTCTCCTCTGGTGGAGACTTTTATGGAACTCACAAGTGGCACTGGTACTTCACCCAGGGTTTTACTGCCATGCTCTTCAGTTTCGTTCCATTTTCCATTTCTGGCATCATCACCTCGAGAAATTGGAAACTTACAGGTCTTATCATCTGGGTTCTGGGACTTCACAGTCTACTAGGTCATAAAGAATTCAGGTTTGTTCTGTCCATTCTTCCAATAACATTGATGTTCTCTGGCTATTCATTAGCCGCCCTCAGATATCCCGCTTCTCCAAATGGTAGAAGGAAAAAAACGGGAGACGTCCACTCCAAACGCCCTATCAAAATTAAGTTGGCCATAATATTTTTGCTAGCTACAAATATCCCAATGGGTTTATACATGAGTTTGGTTCATCAGAGAGGAACAGAGGATGTCATGAATCATCTGGCTCGGAAAGCTGTGAATGAGAAAGTCAAAAGCATCCTCTTTTTGATGCCCTGTCATTCAACGCCTTACTATTCCACTCTGCACCAAAATTTACCAATGCGGTTTCTGGACTGCACACCAAGCGAAGAGAAAGAAATCCCAGTCGAGTCAGACCGTTTCTTGACGAACCCGCTTGATTTTGCAACGGAATTTGCTAAAAATTGGTCTGTCCCAAGTCACATTGTACTGTTTGATTCAGAAGAGAGACTACTCAAGGACTTTCTGATGTCACATTCTTTCAAGGAGGATAGGAGATTCTTCCACGCTCACTTCAAGGTGGACCGCGATCTTCAATCTTCGGTGGTTTTGTATGTCTTGGCCAAC

Protein sequence

MIQKRPRSSAAESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQTYFNPDEHWQALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFMMKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETVLTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRLRFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQGFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGYSLAALRYPASPNGRRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMNHLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNPLDFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLYVLAN
Homology
BLAST of MS002290 vs. NCBI nr
Match: XP_022135464.1 (GPI mannosyltransferase 3 [Momordica charantia])

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 535/543 (98.53%), Postives = 536/543 (98.71%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQ 60
           MIQKRPRSSAAESKSDRIEDSPEFLGSE PNSQKHGLFS PNKVFVFCLAFRIANALLLQ
Sbjct: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAHGI FGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM
Sbjct: 61  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120

Query: 121 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL
Sbjct: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGY 360
           GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVL ILPITLMFSGY
Sbjct: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRYPASPNGRRKKTGDVHSKRPIK+KL IIFLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480
           HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEK IPVESDRFLTNPL
Sbjct: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSH FKEDRRFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 540

Query: 541 LAN 544
           LAN
Sbjct: 541 LAN 543

BLAST of MS002290 vs. NCBI nr
Match: XP_038884088.1 (GPI mannosyltransferase 3 isoform X2 [Benincasa hispida])

HSP 1 Score: 960.7 bits (2482), Expect = 5.4e-276
Identity = 468/541 (86.51%), Postives = 504/541 (93.16%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQ 60
           M+QKRPR+S A SK  RIEDSPEFL SE P S+K+ LFSS NKV V CLAFR ANALLLQ
Sbjct: 1   MVQKRPRTSVAASKLVRIEDSPEFLRSEKPKSKKYDLFSSTNKVLVLCLAFRFANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH ITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLG+DTPL M
Sbjct: 61  TYFNPDEHWQALEVAHNITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGIDTPLIM 120

Query: 121 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +KAPRL+QSIFSA+GDFYLY+LS  LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLQSIFSAVGDFYLYRLSGALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTL SLYYWPCI +A T++SKVSRKLALFMAALSCA+RPTSA TWLYVGLLEL +AHDRL
Sbjct: 181 LTLASLYYWPCISVATTRYSKVSRKLALFMAALSCAIRPTSAATWLYVGLLELFSAHDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLGIT LLDRLMYG WILVP NFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGITFLLDRLMYGSWILVPFNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGY 360
           GFTAMLFSF+PFSISGIITS+ WKL+GLIIWVLG+HSLLGHKEFRFVL ILPI LMFSGY
Sbjct: 301 GFTAMLFSFIPFSISGIITSKRWKLSGLIIWVLGIHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRY ASPNG+R+K+ D+H+KRP+K+ LAII LLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYHASPNGKREKSRDLHTKRPMKMNLAIILLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480
           HLAR+A  EKVK ILFLMPCHSTPYYSTLH+NLPM+FLDCTPSEE  IPVESDRFLTNPL
Sbjct: 421 HLAREAATEKVKRILFLMPCHSTPYYSTLHRNLPMQFLDCTPSEENRIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLYV 540
            FATEFAKNW+VPSHIVLFDSEER+LK+FL+SHSFKED+RFFH+HFKVDRDLQSSVVLYV
Sbjct: 481 AFATEFAKNWTVPSHIVLFDSEERVLKEFLISHSFKEDKRFFHSHFKVDRDLQSSVVLYV 540

Query: 541 L 542
           +
Sbjct: 541 M 541

BLAST of MS002290 vs. NCBI nr
Match: XP_023535348.1 (GPI mannosyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 958.0 bits (2475), Expect = 3.5e-275
Identity = 469/543 (86.37%), Postives = 504/543 (92.82%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQ 60
           M+Q R RSS A S S  I+DSPEFL SE P SQ +  F S NK+FV CLAFR ANALLLQ
Sbjct: 1   MVQIRQRSSVAASNSGGIDDSPEFLPSEKPKSQTYDDFCSTNKLFVLCLAFRFANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH I FGYGHLTWEWKRGIRSY HPL FA LYK+LA+LGLDTPLFM
Sbjct: 61  TYFNPDEHWQALEVAHRIAFGYGHLTWEWKRGIRSYFHPLPFACLYKLLAILGLDTPLFM 120

Query: 121 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +KAPRL++SIFSA+GDFY YKLSS LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLESIFSAVGDFYFYKLSSALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTLVSLYYWPCIR+APT+FSKV RK+ALFMAALSCAVRPTSAITWLYVG+LEL +A DRL
Sbjct: 181 LTLVSLYYWPCIRVAPTRFSKVWRKMALFMAALSCAVRPTSAITWLYVGMLELFSARDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLGIT LLDRLMYG W+ VPLNFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGITFLLDRLMYGSWVFVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGY 360
           GFTAMLFSF+PFSISGII+S+ WKLTGLIIWVLG+HSLLGHKEFRFVL ILPI LMFSGY
Sbjct: 301 GFTAMLFSFIPFSISGIISSKKWKLTGLIIWVLGVHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRYPASPNG+R+K+ DVHSKRP+K+KLA+IFLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYPASPNGKREKSRDVHSKRPMKMKLAVIFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480
           HLA++AV+EKVKSILFLMPCHSTPYYSTLH+NLPMRFLDCTPSEEKEIPVESDRFLTNPL
Sbjct: 421 HLAKEAVSEKVKSILFLMPCHSTPYYSTLHRNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DFA+EFAKNW+  SHIVLF+SEER LKDFL SHSFKED+RFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFASEFAKNWTETSHIVLFESEERPLKDFLKSHSFKEDKRFFHAHFKVDRDLQSSVVLYV 540

Query: 541 LAN 544
           L N
Sbjct: 541 LTN 543

BLAST of MS002290 vs. NCBI nr
Match: XP_038884087.1 (GPI mannosyltransferase 3 isoform X1 [Benincasa hispida])

HSP 1 Score: 956.1 bits (2470), Expect = 1.3e-274
Identity = 468/542 (86.35%), Postives = 504/542 (92.99%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQ 60
           M+QKRPR+S A SK  RIEDSPEFL SE P S+K+ LFSS NKV V CLAFR ANALLLQ
Sbjct: 1   MVQKRPRTSVAASKLVRIEDSPEFLRSEKPKSKKYDLFSSTNKVLVLCLAFRFANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGITFG-YGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLF 120
           TYFNPDEHWQALEVAH ITFG YGHLTWEWKRGIRSYLHPLLFAFLYKVLALLG+DTPL 
Sbjct: 61  TYFNPDEHWQALEVAHNITFGRYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGIDTPLI 120

Query: 121 MMKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLET 180
           M+KAPRL+QSIFSA+GDFYLY+LS  LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLET
Sbjct: 121 MIKAPRLLQSIFSAVGDFYLYRLSGALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLET 180

Query: 181 VLTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDR 240
           VLTL SLYYWPCI +A T++SKVSRKLALFMAALSCA+RPTSA TWLYVGLLEL +AHDR
Sbjct: 181 VLTLASLYYWPCISVATTRYSKVSRKLALFMAALSCAIRPTSAATWLYVGLLELFSAHDR 240

Query: 241 LRFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFT 300
           LRFIFLEAAPIG+LVLGIT LLDRLMYG WILVP NFLKFNVLSSGGDFYGTHKWHWYFT
Sbjct: 241 LRFIFLEAAPIGSLVLGITFLLDRLMYGSWILVPFNFLKFNVLSSGGDFYGTHKWHWYFT 300

Query: 301 QGFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSG 360
           QGFTAMLFSF+PFSISGIITS+ WKL+GLIIWVLG+HSLLGHKEFRFVL ILPI LMFSG
Sbjct: 301 QGFTAMLFSFIPFSISGIITSKRWKLSGLIIWVLGIHSLLGHKEFRFVLPILPIALMFSG 360

Query: 361 YSLAALRYPASPNGRRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVM 420
           YSLAALRY ASPNG+R+K+ D+H+KRP+K+ LAII LLATNIPMGLYMSLVHQRGTEDVM
Sbjct: 361 YSLAALRYHASPNGKREKSRDLHTKRPMKMNLAIILLLATNIPMGLYMSLVHQRGTEDVM 420

Query: 421 NHLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNP 480
           NHLAR+A  EKVK ILFLMPCHSTPYYSTLH+NLPM+FLDCTPSEE  IPVESDRFLTNP
Sbjct: 421 NHLAREAATEKVKRILFLMPCHSTPYYSTLHRNLPMQFLDCTPSEENRIPVESDRFLTNP 480

Query: 481 LDFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLY 540
           L FATEFAKNW+VPSHIVLFDSEER+LK+FL+SHSFKED+RFFH+HFKVDRDLQSSVVLY
Sbjct: 481 LAFATEFAKNWTVPSHIVLFDSEERVLKEFLISHSFKEDKRFFHSHFKVDRDLQSSVVLY 540

Query: 541 VL 542
           V+
Sbjct: 541 VM 542

BLAST of MS002290 vs. NCBI nr
Match: XP_022974975.1 (GPI mannosyltransferase 3 [Cucurbita maxima])

HSP 1 Score: 954.5 bits (2466), Expect = 3.9e-274
Identity = 467/543 (86.00%), Postives = 503/543 (92.63%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQ 60
           M+Q R RSS A S S  IEDSPEFL SE   S+ +  F S NK+FV CLAFR+ANALLLQ
Sbjct: 1   MVQIRQRSSVAASNSGGIEDSPEFLPSEELKSKTYDDFCSTNKLFVLCLAFRVANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH ITFGYGHLTWEWKRGIRSY HPL FA LYK+LA+LGLDTPL M
Sbjct: 61  TYFNPDEHWQALEVAHRITFGYGHLTWEWKRGIRSYFHPLPFACLYKLLAILGLDTPLLM 120

Query: 121 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +KAPRL++SIFSA+GDFY YKLSS LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLESIFSAVGDFYFYKLSSALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTL+SLYYWPCIR+APT+FSKVSRK+ALFMAALSCAVRPTSAITWLYVGLLEL +A DRL
Sbjct: 181 LTLISLYYWPCIRVAPTRFSKVSRKMALFMAALSCAVRPTSAITWLYVGLLELFSARDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLG T +LDRLMYG W+ VPLNFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGFTFVLDRLMYGSWVFVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGY 360
           GFTAMLFSF+PFSISGIITSR WKLTGLIIWVLG+HSLLGHKEFRFVL ILPI LMFSGY
Sbjct: 301 GFTAMLFSFIPFSISGIITSRKWKLTGLIIWVLGIHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRYPAS NG+R+K+ D H K P+K+KLA++FLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYPASLNGKREKSRDAHGKSPMKMKLAVMFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480
           HLA++AV+EKVKSILFLMPCHSTPYYSTLH+NLPMRFLDCTPSEEKEIPVESDRFLTNPL
Sbjct: 421 HLAKEAVSEKVKSILFLMPCHSTPYYSTLHRNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DFA+EFAKNW+VPSHIVLF+SEER LKDFL SHSFKED+RFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFASEFAKNWTVPSHIVLFESEERPLKDFLKSHSFKEDKRFFHAHFKVDRDLQSSVVLYV 540

Query: 541 LAN 544
           LAN
Sbjct: 541 LAN 543

BLAST of MS002290 vs. ExPASy Swiss-Prot
Match: Q94A15 (Mannosyltransferase APTG1 OS=Arabidopsis thaliana OX=3702 GN=APTG1 PE=2 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 4.0e-205
Identity = 349/537 (64.99%), Postives = 422/537 (78.58%), Query Frame = 0

Query: 11  AESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQTYFNPDEHWQ 70
           A+  S  + D P+  G         G   SP ++F+FCLAFR+ NALL+QTYFNPDEHWQ
Sbjct: 17  ADGASVNVGDEPDSFG---------GRIGSPRRIFLFCLAFRVVNALLIQTYFNPDEHWQ 76

Query: 71  ALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFMMKAPRLMQSI 130
           +LEVAH   FGYG++TWEWKRGIRSYLHP+LFAFLYK+L + GLDTP  M+KAPRLMQSI
Sbjct: 77  SLEVAHRTIFGYGYMTWEWKRGIRSYLHPMLFAFLYKLLQVTGLDTPYIMIKAPRLMQSI 136

Query: 131 FSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETVLTLVSLYYWP 190
           FSAIGD YLYKLS  L+G  VA W+LF Q+ NWF+FFC NRT SN LETVLT++ LYYWP
Sbjct: 137 FSAIGDLYLYKLSDALYGGNVATWSLFCQMANWFIFFCLNRTFSNCLETVLTIMGLYYWP 196

Query: 191 CIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRLRFIFLEAAPI 250
           CIR +   +  V+RK  L +AAL+CA+RPTSA+ WLYVG+LEL    ++++FI LE  PI
Sbjct: 197 CIRDSSIDY-PVNRKWGLVIAALACAIRPTSAVIWLYVGMLELFLTPNKVKFIILEVIPI 256

Query: 251 GTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQGFTAMLFSFV 310
           G+LVLG TCLLDRLMYG W++VPLNFLKFN LSSGGD+YGTH WHWYFTQGF  MLF+F 
Sbjct: 257 GSLVLGFTCLLDRLMYGSWVIVPLNFLKFNFLSSGGDYYGTHPWHWYFTQGFLVMLFTFT 316

Query: 311 PFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGYSLAALRYPAS 370
           PFSI+GII S+N KL+ LI+WVL ++S+LGHKEFRFVL +LPI L+FSGY+ A +    S
Sbjct: 317 PFSIAGIIKSKNQKLSALILWVLAIYSILGHKEFRFVLPVLPIALIFSGYAFAQMEVSGS 376

Query: 371 PNG-----RRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMNHLARK 430
            +      +++     H+K   K++L++ FLLATNIPM LYMSL HQRGTED MN+L+ +
Sbjct: 377 SSSSSVTKKKQVPRQNHTKWSPKLRLSVYFLLATNIPMALYMSLFHQRGTEDAMNYLSDE 436

Query: 431 AVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNPLDFATE 490
           A   +VKSILFLMPCHSTPYYSTLH+N+PM+FLDCTPS EK    ESD+FL NPL FA+E
Sbjct: 437 AYKGRVKSILFLMPCHSTPYYSTLHRNIPMQFLDCTPSAEKGELDESDQFLVNPLGFASE 496

Query: 491 FAKNWS-VPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLYVL 542
            A+NWS  PSHIVLF SEE  L+DF++ HSFKE RRFFHAHFKVDRDLQSSVV+YV+
Sbjct: 497 LARNWSEPPSHIVLFASEETKLRDFMIQHSFKEVRRFFHAHFKVDRDLQSSVVVYVV 543

BLAST of MS002290 vs. ExPASy Swiss-Prot
Match: Q9JJQ0 (GPI mannosyltransferase 3 OS=Mus musculus OX=10090 GN=Pigb PE=2 SV=2)

HSP 1 Score: 378.3 bits (970), Expect = 1.5e-103
Identity = 209/526 (39.73%), Postives = 302/526 (57.41%), Query Frame = 0

Query: 9   SAAESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQTYFNPDEH 68
           S+ E K  R   S +++ ++   S + GL      + +F +A RI N  L+QT F PDE+
Sbjct: 18  SSGEMKLRR-RKSRQYVSAQEKRSPRRGLLGENTYLVLFTIALRILNCFLVQTSFVPDEY 77

Query: 69  WQALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFMMKAPRLMQ 128
           WQ+LEVAH + F YG+LTWEW   +R Y +PL+FA +YKVL LLG D+  F++  PRL Q
Sbjct: 78  WQSLEVAHRMVFSYGYLTWEWTERLRGYTYPLIFASIYKVLHLLGKDSVQFLIWIPRLGQ 137

Query: 129 SIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETVLTLVSLYY 188
           ++ SA+ D  LY L   L    VA+W    QL +WF ++C  RTL+N++ET LT ++L+Y
Sbjct: 138 ALLSAVADIRLYSLLKQLENQEVAQWVFLCQLCSWFTWYCCTRTLTNTMETSLTALALFY 197

Query: 189 WPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRLRFIFLEAA 248
           +P          K S      + AL+C VRPT+ I W+ +          +L        
Sbjct: 198 YPLEGSRSVNSVKYS-----LLVALACVVRPTALIPWVPLLFRHFYQEQRKLHLTLHHFL 257

Query: 249 PIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQGFTAMLFS 308
           P+G +   ++ ++DR+ +G W LV LNFLKFNVL + G FYG+H WHWY +QGF  +L +
Sbjct: 258 PVGFITFSLSLIIDRIFFGQWTLVQLNFLKFNVLQNLGTFYGSHPWHWYLSQGFPVVLGT 317

Query: 309 FVPFSISG--IITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGYSLAALR 368
            +PF I G  +   R   L   ++W L ++S+LGHKEFRF+  +LP  ++F GYSLA L+
Sbjct: 318 HLPFFIHGCFLAPRRLHILLLTVLWTLLVYSMLGHKEFRFIYPVLPFCMVFCGYSLAHLK 377

Query: 369 YPASPNGRRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMNHLAR-- 428
                                  K A+ FLL +N+P+  Y  LVHQRGT DVMNH+ +  
Sbjct: 378 --------------------TWRKAALSFLLLSNVPLAFYTGLVHQRGTLDVMNHIQKVC 437

Query: 429 -KAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTP--SEEKEIPVESDRFLTNPLD 488
            +  +    S+  +MPCHSTPYYS +H  L MRFL C P  + + +   E+D F  NPL 
Sbjct: 438 PRGPDPASASVFIMMPCHSTPYYSHVHCPLSMRFLQCPPDLTGKTQYLDEADMFYLNPLR 497

Query: 489 F-ATEFAKNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHF 527
           +   EF  N S+P+H+V F+  E+ +  FL S +++    FFH H+
Sbjct: 498 WLQQEFHSNASLPTHLVTFNVLEKEINTFLTSGNYERAATFFHTHW 517

BLAST of MS002290 vs. ExPASy Swiss-Prot
Match: Q1LZA0 (GPI mannosyltransferase 3 OS=Bos taurus OX=9913 GN=PIGB PE=2 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 5.7e-103
Identity = 205/520 (39.42%), Postives = 300/520 (57.69%), Query Frame = 0

Query: 32  SQKHGLFSSPNK-------------VFVFCLAFRIANALLLQTYFNPDEHWQALEVAHGI 91
           ++K  L+ SP K             + +F +A RI N  L+QT F PDE+WQ+LEVAH +
Sbjct: 28  TRKSTLYLSPQKSTGRCGDLGENIYLVLFIIALRICNCFLVQTSFVPDEYWQSLEVAHRM 87

Query: 92  TFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFMMKAPRLMQSIFSAIGDFY 151
            F YG+LTWEW   +R Y +PL+FA +YK+L LLG D+   ++  PRL Q++ SAI D  
Sbjct: 88  VFNYGYLTWEWTERLRGYTYPLIFASIYKILHLLGKDSVHLLIWIPRLAQALLSAIADLR 147

Query: 152 LYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETVLTLVSLYYWPCIRIAPTK 211
           LY L   L    VA+W  F QL +WF ++C  RTL+N++ETVLT+++L+Y+P        
Sbjct: 148 LYSLMKQLENQQVARWVFFCQLCSWFTWYCCTRTLTNTMETVLTIIALFYYPLEGSKSMN 207

Query: 212 FSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRLRFIFLEAAPIGTLVLGIT 271
             K S      + AL+  +RPT+ I W+ +          +L  I  +  P+G + L ++
Sbjct: 208 SVKYSS-----LVALAFIIRPTAVIPWIPLLFRHFWQEQRKLDLILHQFLPVGFVTLSLS 267

Query: 272 CLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQGFTAMLFSFVPFSISG-I 331
            ++DR+ +G W LV  NFLKFNVL   G FYG+H WHWYF+QGF A+L + +PF I G  
Sbjct: 268 LIIDRIFFGQWTLVQYNFLKFNVLQDLGSFYGSHPWHWYFSQGFPAVLGTHLPFFIHGCF 327

Query: 332 ITSRNWKL-TGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGYSLAALRYPASPNGRRK 391
           +  + +++    ++W L ++S+L HKEFRF+  +LP  ++F GYSL  L+    P     
Sbjct: 328 LAPKRYRIFLVTVLWTLLVYSMLSHKEFRFIYPVLPFCMVFCGYSLNNLKTWKKP----- 387

Query: 392 KTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMNH---LARKAVNEKVKS 451
                          A+ FL  +N+ + LY  LVHQRGT DVM +   L+    N    S
Sbjct: 388 ---------------ALSFLFLSNMLLALYTGLVHQRGTLDVMTNIQELSYNNTNVSSAS 447

Query: 452 ILFLMPCHSTPYYSTLHQNLPMRFLDCTP--SEEKEIPVESDRFLTNPLDFA-TEFAKNW 511
           +L +MPCHSTPYYS +H  LPMRFL C P  + + +  VE+D F  NPL +   EF  + 
Sbjct: 448 VLMMMPCHSTPYYSHVHYPLPMRFLQCPPDLTGKTDYLVEADMFYLNPLKWLYMEFQNDS 507

Query: 512 SVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDR 531
            +P+H+++F   E  +  FL+S++++    FFH HF   R
Sbjct: 508 QLPTHLIMFSVLEEEISPFLISNNYERTAVFFHTHFPESR 522

BLAST of MS002290 vs. ExPASy Swiss-Prot
Match: Q92521 (GPI mannosyltransferase 3 OS=Homo sapiens OX=9606 GN=PIGB PE=1 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 5.7e-103
Identity = 208/532 (39.10%), Postives = 304/532 (57.14%), Query Frame = 0

Query: 17  RIEDSPEFLGSENPNSQKHG-LFSSPNKVFVFCLAFRIANALLLQTYFNPDEHWQALEVA 76
           R   S  +  ++  ++++ G L      + +F +A RI N  L+QT F PDE+WQ+LEV+
Sbjct: 35  RKRKSTLYFNTQEKSARRRGDLLGENIYLLLFTIALRILNCFLVQTSFVPDEYWQSLEVS 94

Query: 77  HGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFMMKAPRLMQSIFSAIG 136
           H + F YG+LTWEW   +RSY +PL+FA +YK+L LLG D+   ++  PRL Q++ SA+ 
Sbjct: 95  HHMVFNYGYLTWEWTERLRSYTYPLIFASIYKILHLLGKDSVQLLIWIPRLAQALLSAVA 154

Query: 137 DFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETVLTLVSLYYWPCIRIA 196
           D  LY L   L    VA+W  F QL +WF ++C  RTL+N++ETVLT+++L+Y+P     
Sbjct: 155 DVRLYSLMKQLENQEVARWVFFCQLCSWFTWYCCTRTLTNTMETVLTIIALFYYPLEGSK 214

Query: 197 PTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRLRFIFLEAAPIGTLVL 256
                K S      + AL+  +RPT+ I W  +     C    +L  I     P+G + L
Sbjct: 215 SMNSVKYSS-----LVALAFIIRPTAVILWTPLLFRHFCQEPRKLDLILHHFLPVGFVTL 274

Query: 257 GITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQGFTAMLFSFVPFSIS 316
            ++ ++DR+ +G W LV  NFLKFNVL + G FYG+H WHWYF+QGF  +L + +PF I 
Sbjct: 275 SLSLMIDRIFFGQWTLVQFNFLKFNVLQNWGTFYGSHPWHWYFSQGFPVILGTHLPFFIH 334

Query: 317 G--IITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGYSLAALRYPASPNG 376
           G  +   R   L   ++W L ++S+L HKEFRF+  +LP  ++F GYSL  L+    P  
Sbjct: 335 GCYLAPKRYRILLVTVLWTLLVYSMLSHKEFRFIYPVLPFCMVFCGYSLTHLKTWKKP-- 394

Query: 377 RRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMNHLARKAVNEKVK- 436
                             A+ FL  +N+ + LY  LVHQRGT DVM+H+ +   N   K 
Sbjct: 395 ------------------ALSFLFLSNLFLALYTGLVHQRGTLDVMSHIQKVCYNNPNKS 454

Query: 437 --SILFLMPCHSTPYYSTLHQNLPMRFLDCTP--SEEKEIPVESDRFLTNPLDFA-TEFA 496
             SI  +MPCHSTPYYS +H  LPMRFL C P  + +     E+D F  NPL++   EF 
Sbjct: 455 SASIFIMMPCHSTPYYSHVHCPLPMRFLQCPPDLTGKSHYLDEADVFYLNPLNWLHREFH 514

Query: 497 KNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLY 540
            + S+P+H++ F   E  +  FL+S ++K    FFH H    R + S + +Y
Sbjct: 515 DDASLPTHLITFSILEEEISAFLISSNYKRTAVFFHTHLPEGR-IGSHIYVY 540

BLAST of MS002290 vs. ExPASy Swiss-Prot
Match: Q7SXZ1 (GPI mannosyltransferase 3 OS=Danio rerio OX=7955 GN=pigb PE=2 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 7.3e-98
Identity = 213/547 (38.94%), Postives = 311/547 (56.86%), Query Frame = 0

Query: 2   IQKRPRSSAAESKSDRIEDSPEFL-GSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQ 61
           I++R + S + ++S R+      L  +++   Q   +F     + VF +  R+ N +L+Q
Sbjct: 4   IRERFKQSKSSNESVRLRKRSSILYSTDDRKLQTLKIFGI--NITVFIVLVRLLNCVLVQ 63

Query: 62  TYFNPDEHWQALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 121
           T F PDE WQ+LE++H + FGYG+ TWEWK GIR Y +PLLFA +YK+L L+  DT   +
Sbjct: 64  TSFVPDEFWQSLEISHRMVFGYGYETWEWKEGIRGYSYPLLFALMYKLLQLISYDTVYLL 123

Query: 122 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 181
           +  PR+ Q++ +A  D  LYKL        VAKW  F QL +WF +FC +RTL+N+ ET 
Sbjct: 124 VFVPRVFQALLAAYADVKLYKLILQWETPDVAKWTCFCQLCSWFAWFCCSRTLTNTTETA 183

Query: 182 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 241
           LT ++L ++P     P   +  S K  L + +L+  +RPT+ I W  +     CT  D+L
Sbjct: 184 LTTLALCFYP----LPGSKTHSSWKY-LTLVSLAVVIRPTALIVWFPLIFYHFCTDQDKL 243

Query: 242 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 301
           + I     PIG L LG++ L+D   YG WI V  NFLKFNVL + G+FYG+H WHWY+TQ
Sbjct: 244 KLITHRYFPIGVLALGVSTLIDSFFYGKWIFVQWNFLKFNVLHNVGEFYGSHPWHWYWTQ 303

Query: 302 GFTAMLFSFVPFSISGII--TSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFS 361
           G   ++   +P  + G +  T ++  L   IIW   ++SLL HKEFRF+  +LP  ++F 
Sbjct: 304 GLPVVIGPHLPLVLHGCLLSTRKHTILLLTIIWTTAVYSLLAHKEFRFIYPVLPFCMIFC 363

Query: 362 GYSLAALRYPASPNGRRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDV 421
           G SLA L+                       K A   LL  N+   LY  LVHQRG  DV
Sbjct: 364 GLSLAKLQ--------------------AWRKAAAGALLLFNLCPALYTGLVHQRGALDV 423

Query: 422 MNHLARKAVNEKVKS---ILFLMPCHSTPYYSTLHQNLPMRFLDCTP--SEEKEIPVESD 481
           M+ L    V+ +  +   +LFLMPCHSTP YS LH  L +RFL+C P  +  ++   E+D
Sbjct: 424 MHVLQPLCVSSQSPNPPELLFLMPCHSTPLYSHLHCPLKLRFLECPPDLTGRQDYMDEAD 483

Query: 482 RFLTNPLDFA-TEFAKNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDL 540
            F ++PL +  T F    ++PSH+VLFDS ++ +  FL  +SF +    FH HF   R +
Sbjct: 484 VFFSDPLHWLNTSFPLRSTLPSHLVLFDSLQKEISSFLEENSFAKQAEIFHTHFPEGR-V 522

BLAST of MS002290 vs. ExPASy TrEMBL
Match: A0A6J1C1I0 (Mannosyltransferase OS=Momordica charantia OX=3673 GN=LOC111007416 PE=3 SV=1)

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 535/543 (98.53%), Postives = 536/543 (98.71%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQ 60
           MIQKRPRSSAAESKSDRIEDSPEFLGSE PNSQKHGLFS PNKVFVFCLAFRIANALLLQ
Sbjct: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSEKPNSQKHGLFSLPNKVFVFCLAFRIANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAHGI FGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM
Sbjct: 61  TYFNPDEHWQALEVAHGIAFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120

Query: 121 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL
Sbjct: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGY 360
           GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVL ILPITLMFSGY
Sbjct: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLPILPITLMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRYPASPNGRRKKTGDVHSKRPIK+KL IIFLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKMKLTIIFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480
           HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEK IPVESDRFLTNPL
Sbjct: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKGIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSH FKEDRRFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHYFKEDRRFFHAHFKVDRDLQSSVVLYV 540

Query: 541 LAN 544
           LAN
Sbjct: 541 LAN 543

BLAST of MS002290 vs. ExPASy TrEMBL
Match: A0A6J1IFD5 (Mannosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111473821 PE=3 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.9e-274
Identity = 467/543 (86.00%), Postives = 503/543 (92.63%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQ 60
           M+Q R RSS A S S  IEDSPEFL SE   S+ +  F S NK+FV CLAFR+ANALLLQ
Sbjct: 1   MVQIRQRSSVAASNSGGIEDSPEFLPSEELKSKTYDDFCSTNKLFVLCLAFRVANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH ITFGYGHLTWEWKRGIRSY HPL FA LYK+LA+LGLDTPL M
Sbjct: 61  TYFNPDEHWQALEVAHRITFGYGHLTWEWKRGIRSYFHPLPFACLYKLLAILGLDTPLLM 120

Query: 121 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +KAPRL++SIFSA+GDFY YKLSS LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLESIFSAVGDFYFYKLSSALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTL+SLYYWPCIR+APT+FSKVSRK+ALFMAALSCAVRPTSAITWLYVGLLEL +A DRL
Sbjct: 181 LTLISLYYWPCIRVAPTRFSKVSRKMALFMAALSCAVRPTSAITWLYVGLLELFSARDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLG T +LDRLMYG W+ VPLNFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGFTFVLDRLMYGSWVFVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGY 360
           GFTAMLFSF+PFSISGIITSR WKLTGLIIWVLG+HSLLGHKEFRFVL ILPI LMFSGY
Sbjct: 301 GFTAMLFSFIPFSISGIITSRKWKLTGLIIWVLGIHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRYPAS NG+R+K+ D H K P+K+KLA++FLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYPASLNGKREKSRDAHGKSPMKMKLAVMFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480
           HLA++AV+EKVKSILFLMPCHSTPYYSTLH+NLPMRFLDCTPSEEKEIPVESDRFLTNPL
Sbjct: 421 HLAKEAVSEKVKSILFLMPCHSTPYYSTLHRNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DFA+EFAKNW+VPSHIVLF+SEER LKDFL SHSFKED+RFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFASEFAKNWTVPSHIVLFESEERPLKDFLKSHSFKEDKRFFHAHFKVDRDLQSSVVLYV 540

Query: 541 LAN 544
           LAN
Sbjct: 541 LAN 543

BLAST of MS002290 vs. ExPASy TrEMBL
Match: A0A6J1F8Z8 (Mannosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443205 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 7.2e-274
Identity = 465/543 (85.64%), Postives = 503/543 (92.63%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQ 60
           M+Q R RSS A S S  I+DSPEFL SE P S+ +  F S NK+ V CLAFR ANALLLQ
Sbjct: 1   MVQIRQRSSVAASNSGGIDDSPEFLPSEKPKSKTYDDFCSTNKLLVLCLAFRFANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH I FGYGHLTWEWKRGIRSY HPL FA LYK+LA+LGLDTPLFM
Sbjct: 61  TYFNPDEHWQALEVAHRIAFGYGHLTWEWKRGIRSYFHPLPFACLYKLLAILGLDTPLFM 120

Query: 121 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +KAPRL++SIFSA GDFY YKLSS LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLESIFSAAGDFYFYKLSSALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTLVSLYYWPCIR+APT+ SKVSRK+ALFMAALSCA+RPTSAITWLYVG+LEL +A DRL
Sbjct: 181 LTLVSLYYWPCIRVAPTRSSKVSRKMALFMAALSCAIRPTSAITWLYVGMLELFSARDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLGIT LLDRLMYG W+ VPLNFLKFNVLSSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGITFLLDRLMYGSWVFVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGY 360
           GFTAMLFSF+PFSISGII+S+ WKLTGL+IWVLG+HSLLGHKEFRFVL ILPI LMFSGY
Sbjct: 301 GFTAMLFSFIPFSISGIISSKKWKLTGLVIWVLGVHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRYPASPNG+R+K+ DVHSK P+K+KLA+IFLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYPASPNGKREKSRDVHSKCPMKMKLAVIFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480
           HLA++AV+EKVKSILFLMPCHSTPYYSTLH+NLPMRFLDCTPSEE+EIPVESDRFLTNPL
Sbjct: 421 HLAKEAVSEKVKSILFLMPCHSTPYYSTLHRNLPMRFLDCTPSEEREIPVESDRFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DFA+EFAKNW+VPSHIVLF+SEER LKDFL SHSFKED+RFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFASEFAKNWTVPSHIVLFESEERPLKDFLKSHSFKEDKRFFHAHFKVDRDLQSSVVLYV 540

Query: 541 LAN 544
           L N
Sbjct: 541 LTN 543

BLAST of MS002290 vs. ExPASy TrEMBL
Match: A0A0A0LTJ6 (Mannosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G270410 PE=3 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.1e-266
Identity = 452/541 (83.55%), Postives = 494/541 (91.31%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQ 60
           M++KRPR+  + +K  R EDSPE L SE    +++G+F S NKV +FCLAFR ANALLLQ
Sbjct: 1   MVEKRPRTMLSTAKLVRNEDSPELLRSEKRKFKRYGVFFSTNKVLLFCLAFRFANALLLQ 60

Query: 61  TYFNPDEHWQALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH ITFGYGHLTWEWKRGIRSYLHPLLFA LYK+LALLG+DTPL M
Sbjct: 61  TYFNPDEHWQALEVAHDITFGYGHLTWEWKRGIRSYLHPLLFACLYKLLALLGIDTPLLM 120

Query: 121 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +KAPRL+QSIFSA+GDFYLY+LS  LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLQSIFSAVGDFYLYRLSGALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTL SLYYWPCI +APT+ SKVSRKLALFMAALSCA+RPTSAITWLYVGLLEL +AHD+L
Sbjct: 181 LTLASLYYWPCISVAPTRISKVSRKLALFMAALSCAIRPTSAITWLYVGLLELFSAHDQL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLGIT LLDR+MYG W LVPLNFL+FNV SSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGITFLLDRVMYGSWTLVPLNFLRFNVFSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGY 360
           GF+AMLFSF+PFSISGIITSR WKL+GLI+WVLG+HSLLGHKEFRFVL ILPI LMFSGY
Sbjct: 301 GFSAMLFSFIPFSISGIITSRKWKLSGLIVWVLGIHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALRY  S NG+R K+ D+H+KRP+K+ LAIIFLL TNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRYQTSSNGKRAKSRDLHTKRPMKMNLAIIFLLTTNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480
           HLAR+AV  KVK ILFLMPCHSTPYYSTLHQNLPM+FLDCTPS EK  PVES+RFLTNPL
Sbjct: 421 HLAREAVTGKVKRILFLMPCHSTPYYSTLHQNLPMQFLDCTPSVEKGSPVESERFLTNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DF+ EFAKNW+VPSHIVLFDSEERLL DFL+SHSFKED+RFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFSIEFAKNWTVPSHIVLFDSEERLLMDFLISHSFKEDKRFFHAHFKVDRDLQSSVVLYV 540

Query: 541 L 542
           L
Sbjct: 541 L 541

BLAST of MS002290 vs. ExPASy TrEMBL
Match: A0A1S3CU16 (Mannosyltransferase OS=Cucumis melo OX=3656 GN=LOC103504543 PE=3 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 8.8e-264
Identity = 447/541 (82.62%), Postives = 490/541 (90.57%), Query Frame = 0

Query: 1   MIQKRPRSSAAESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQ 60
           M+QKRP++    SK  R EDSPE L SE P S+++ LF S NKV +FCLAFR ANA LLQ
Sbjct: 1   MVQKRPKTLVTTSKLVRNEDSPELLRSEKPKSKRYDLFFSMNKVLLFCLAFRFANAFLLQ 60

Query: 61  TYFNPDEHWQALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFM 120
           TYFNPDEHWQALEVAH ITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLG+DTPL M
Sbjct: 61  TYFNPDEHWQALEVAHYITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGVDTPLLM 120

Query: 121 MKAPRLMQSIFSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180
           +KAPRL+QSIFSA+GDFYLY+LS  LFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV
Sbjct: 121 IKAPRLLQSIFSAVGDFYLYRLSRALFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETV 180

Query: 181 LTLVSLYYWPCIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRL 240
           LTL SLYYWPCI + PT+ SKVSRKLALFMAALSC +RPTSAITWLYVGLLEL +A+DRL
Sbjct: 181 LTLASLYYWPCISVVPTRISKVSRKLALFMAALSCGIRPTSAITWLYVGLLELFSANDRL 240

Query: 241 RFIFLEAAPIGTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQ 300
           RFIFLEAAPIG+LVLGIT LLDR+MYG W LVPLNFL+FNV SSGGDFYGTHKWHWYFTQ
Sbjct: 241 RFIFLEAAPIGSLVLGITFLLDRVMYGSWTLVPLNFLRFNVFSSGGDFYGTHKWHWYFTQ 300

Query: 301 GFTAMLFSFVPFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGY 360
           GF+AMLFSF+PFSISGI+ SR WKL+GL++WVLG+HSLLGHKEFRFVL ILPI LMFSGY
Sbjct: 301 GFSAMLFSFIPFSISGIVASRKWKLSGLLVWVLGIHSLLGHKEFRFVLPILPIALMFSGY 360

Query: 361 SLAALRYPASPNGRRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMN 420
           SLAALR   SPN +R+K+ ++H+KRP+K+ LA IFLLATNIPMGLYMSLVHQRGTEDVMN
Sbjct: 361 SLAALRSQTSPNSKREKSRNLHTKRPMKMSLATIFLLATNIPMGLYMSLVHQRGTEDVMN 420

Query: 421 HLARKAVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNPL 480
           HLAR+A N KVK ILFLMPCHSTPYYSTLHQNLPM+FLDCTPS EK   VES+RFL NPL
Sbjct: 421 HLAREAANGKVKRILFLMPCHSTPYYSTLHQNLPMQFLDCTPSVEKGSLVESERFLMNPL 480

Query: 481 DFATEFAKNWSVPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLYV 540
           DF+ EFAKNW+VPSHIVLFDSEERLLKDFL+SHSFKED+RFFHAHFKVDRDLQSSVVLYV
Sbjct: 481 DFSIEFAKNWTVPSHIVLFDSEERLLKDFLISHSFKEDKRFFHAHFKVDRDLQSSVVLYV 540

Query: 541 L 542
           +
Sbjct: 541 I 541

BLAST of MS002290 vs. TAIR 10
Match: AT5G14850.1 (Alg9-like mannosyltransferase family )

HSP 1 Score: 715.7 bits (1846), Expect = 2.8e-206
Identity = 349/537 (64.99%), Postives = 422/537 (78.58%), Query Frame = 0

Query: 11  AESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQTYFNPDEHWQ 70
           A+  S  + D P+  G         G   SP ++F+FCLAFR+ NALL+QTYFNPDEHWQ
Sbjct: 17  ADGASVNVGDEPDSFG---------GRIGSPRRIFLFCLAFRVVNALLIQTYFNPDEHWQ 76

Query: 71  ALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFMMKAPRLMQSI 130
           +LEVAH   FGYG++TWEWKRGIRSYLHP+LFAFLYK+L + GLDTP  M+KAPRLMQSI
Sbjct: 77  SLEVAHRTIFGYGYMTWEWKRGIRSYLHPMLFAFLYKLLQVTGLDTPYIMIKAPRLMQSI 136

Query: 131 FSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETVLTLVSLYYWP 190
           FSAIGD YLYKLS  L+G  VA W+LF Q+ NWF+FFC NRT SN LETVLT++ LYYWP
Sbjct: 137 FSAIGDLYLYKLSDALYGGNVATWSLFCQMANWFIFFCLNRTFSNCLETVLTIMGLYYWP 196

Query: 191 CIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRLRFIFLEAAPI 250
           CIR +   +  V+RK  L +AAL+CA+RPTSA+ WLYVG+LEL    ++++FI LE  PI
Sbjct: 197 CIRDSSIDY-PVNRKWGLVIAALACAIRPTSAVIWLYVGMLELFLTPNKVKFIILEVIPI 256

Query: 251 GTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQGFTAMLFSFV 310
           G+LVLG TCLLDRLMYG W++VPLNFLKFN LSSGGD+YGTH WHWYFTQGF  MLF+F 
Sbjct: 257 GSLVLGFTCLLDRLMYGSWVIVPLNFLKFNFLSSGGDYYGTHPWHWYFTQGFLVMLFTFT 316

Query: 311 PFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGYSLAALRYPAS 370
           PFSI+GII S+N KL+ LI+WVL ++S+LGHKEFRFVL +LPI L+FSGY+ A +    S
Sbjct: 317 PFSIAGIIKSKNQKLSALILWVLAIYSILGHKEFRFVLPVLPIALIFSGYAFAQMEVSGS 376

Query: 371 PNG-----RRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMNHLARK 430
            +      +++     H+K   K++L++ FLLATNIPM LYMSL HQRGTED MN+L+ +
Sbjct: 377 SSSSSVTKKKQVPRQNHTKWSPKLRLSVYFLLATNIPMALYMSLFHQRGTEDAMNYLSDE 436

Query: 431 AVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNPLDFATE 490
           A   +VKSILFLMPCHSTPYYSTLH+N+PM+FLDCTPS EK    ESD+FL NPL FA+E
Sbjct: 437 AYKGRVKSILFLMPCHSTPYYSTLHRNIPMQFLDCTPSAEKGELDESDQFLVNPLGFASE 496

Query: 491 FAKNWS-VPSHIVLFDSEERLLKDFLMSHSFKEDRRFFHAHFKVDRDLQSSVVLYVL 542
            A+NWS  PSHIVLF SEE  L+DF++ HSFKE RRFFHAHFKVDRDLQSSVV+YV+
Sbjct: 497 LARNWSEPPSHIVLFASEETKLRDFMIQHSFKEVRRFFHAHFKVDRDLQSSVVVYVV 543

BLAST of MS002290 vs. TAIR 10
Match: AT5G14850.2 (Alg9-like mannosyltransferase family )

HSP 1 Score: 676.8 bits (1745), Expect = 1.5e-194
Identity = 330/517 (63.83%), Postives = 401/517 (77.56%), Query Frame = 0

Query: 11  AESKSDRIEDSPEFLGSENPNSQKHGLFSSPNKVFVFCLAFRIANALLLQTYFNPDEHWQ 70
           A+  S  + D P+  G         G   SP ++F+FCLAFR+ NALL+QTYFNPDEHWQ
Sbjct: 17  ADGASVNVGDEPDSFG---------GRIGSPRRIFLFCLAFRVVNALLIQTYFNPDEHWQ 76

Query: 71  ALEVAHGITFGYGHLTWEWKRGIRSYLHPLLFAFLYKVLALLGLDTPLFMMKAPRLMQSI 130
           +LEVAH   FGYG++TWEWKRGIRSYLHP+LFAFLYK+L + GLDTP  M+KAPRLMQSI
Sbjct: 77  SLEVAHRTIFGYGYMTWEWKRGIRSYLHPMLFAFLYKLLQVTGLDTPYIMIKAPRLMQSI 136

Query: 131 FSAIGDFYLYKLSSVLFGDYVAKWALFSQLTNWFMFFCFNRTLSNSLETVLTLVSLYYWP 190
           FSAIGD YLYKLS  L+G  VA W+LF Q+ NWF+FFC NRT SN LETVLT++ LYYWP
Sbjct: 137 FSAIGDLYLYKLSDALYGGNVATWSLFCQMANWFIFFCLNRTFSNCLETVLTIMGLYYWP 196

Query: 191 CIRIAPTKFSKVSRKLALFMAALSCAVRPTSAITWLYVGLLELCTAHDRLRFIFLEAAPI 250
           CIR +   +  V+RK  L +AAL+CA+RPTSA+ WLYVG+LEL    ++++FI LE  PI
Sbjct: 197 CIRDSSIDY-PVNRKWGLVIAALACAIRPTSAVIWLYVGMLELFLTPNKVKFIILEVIPI 256

Query: 251 GTLVLGITCLLDRLMYGFWILVPLNFLKFNVLSSGGDFYGTHKWHWYFTQGFTAMLFSFV 310
           G+LVLG TCLLDRLMYG W++VPLNFLKFN LSSGGD+YGTH WHWYFTQGF  MLF+F 
Sbjct: 257 GSLVLGFTCLLDRLMYGSWVIVPLNFLKFNFLSSGGDYYGTHPWHWYFTQGFLVMLFTFT 316

Query: 311 PFSISGIITSRNWKLTGLIIWVLGLHSLLGHKEFRFVLSILPITLMFSGYSLAALRYPAS 370
           PFSI+GII S+N KL+ LI+WVL ++S+LGHKEFRFVL +LPI L+FSGY+ A +    S
Sbjct: 317 PFSIAGIIKSKNQKLSALILWVLAIYSILGHKEFRFVLPVLPIALIFSGYAFAQMEVSGS 376

Query: 371 PNG-----RRKKTGDVHSKRPIKIKLAIIFLLATNIPMGLYMSLVHQRGTEDVMNHLARK 430
            +      +++     H+K   K++L++ FLLATNIPM LYMSL HQRGTED MN+L+ +
Sbjct: 377 SSSSSVTKKKQVPRQNHTKWSPKLRLSVYFLLATNIPMALYMSLFHQRGTEDAMNYLSDE 436

Query: 431 AVNEKVKSILFLMPCHSTPYYSTLHQNLPMRFLDCTPSEEKEIPVESDRFLTNPLDFATE 490
           A   +VKSILFLMPCHSTPYYSTLH+N+PM+FLDCTPS EK    ESD+FL NPL FA+E
Sbjct: 437 AYKGRVKSILFLMPCHSTPYYSTLHRNIPMQFLDCTPSAEKGELDESDQFLVNPLGFASE 496

Query: 491 FAKNWS-VPSHIVLFDSEERLLKDFLMSHSFKEDRRF 522
            A+NWS  PSHIVLF SEE  L+DF++ HSFKE R F
Sbjct: 497 LARNWSEPPSHIVLFASEETKLRDFMIQHSFKEARGF 523

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135464.10.0e+0098.53GPI mannosyltransferase 3 [Momordica charantia][more]
XP_038884088.15.4e-27686.51GPI mannosyltransferase 3 isoform X2 [Benincasa hispida][more]
XP_023535348.13.5e-27586.37GPI mannosyltransferase 3 [Cucurbita pepo subsp. pepo][more]
XP_038884087.11.3e-27486.35GPI mannosyltransferase 3 isoform X1 [Benincasa hispida][more]
XP_022974975.13.9e-27486.00GPI mannosyltransferase 3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q94A154.0e-20564.99Mannosyltransferase APTG1 OS=Arabidopsis thaliana OX=3702 GN=APTG1 PE=2 SV=1[more]
Q9JJQ01.5e-10339.73GPI mannosyltransferase 3 OS=Mus musculus OX=10090 GN=Pigb PE=2 SV=2[more]
Q1LZA05.7e-10339.42GPI mannosyltransferase 3 OS=Bos taurus OX=9913 GN=PIGB PE=2 SV=1[more]
Q925215.7e-10339.10GPI mannosyltransferase 3 OS=Homo sapiens OX=9606 GN=PIGB PE=1 SV=1[more]
Q7SXZ17.3e-9838.94GPI mannosyltransferase 3 OS=Danio rerio OX=7955 GN=pigb PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C1I00.0e+0098.53Mannosyltransferase OS=Momordica charantia OX=3673 GN=LOC111007416 PE=3 SV=1[more]
A0A6J1IFD51.9e-27486.00Mannosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111473821 PE=3 SV=1[more]
A0A6J1F8Z87.2e-27485.64Mannosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443205 PE=3 SV=1[more]
A0A0A0LTJ61.1e-26683.55Mannosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G270410 PE=3 SV=1[more]
A0A1S3CU168.8e-26482.62Mannosyltransferase OS=Cucumis melo OX=3656 GN=LOC103504543 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G14850.12.8e-20664.99Alg9-like mannosyltransferase family [more]
AT5G14850.21.5e-19463.83Alg9-like mannosyltransferase family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005599GPI mannosyltransferasePFAMPF03901Glyco_transf_22coord: 44..450
e-value: 1.9E-112
score: 376.6
IPR005599GPI mannosyltransferasePANTHERPTHR22760GLYCOSYLTRANSFERASEcoord: 44..539
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 9..23
IPR039521GPI mannosyltransferase 3PANTHERPTHR22760:SF4GPI MANNOSYLTRANSFERASE 3coord: 44..539

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002290.1MS002290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006506 GPI anchor biosynthetic process
molecular_function GO:0000026 alpha-1,2-mannosyltransferase activity
molecular_function GO:0004376 glycolipid mannosyltransferase activity
molecular_function GO:0016757 glycosyltransferase activity