MS002257 (gene) Bitter gourd (TR) v1

Overview
NameMS002257
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionXyloglucan endotransglucosylase/hydrolase
Locationscaffold30: 3657596 .. 3658647 (+)
RNA-Seq ExpressionMS002257
SyntenyMS002257
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATATCCAATGCCTCCTCCTGCTTTGTCACTCCTGCTCTTTCTGACTCTCTCTGTTTCCTCTGTTTTTGCTGCCAACTTCAACCAGGATGTTGAGCTCATTTGGGGCAATGAACGTGCCAATATCCTTGACGATGGCCACCTCCTCACTCTCTCCTTGGACCGAGCCTCTGGCTCCGGCTTCCAGTCCAAAAATGTTTACCTTTTTGGTAAAATTGAGATGCAGCTCAAACTTGTCCCTGGAAACTCCGCTGGCACTGTCACCACTTACTATGTATGAATATAAACTGATTTACTTTATCCAACCCAACCCAATTGCCTTGCACACACATGTTTCTGATTTCATATATATATATATATATATATTTGTTTTGTATGCAGCTTAAATCCGAAGGGTCGACGTGGGATGAGATAGATTTTGAATTCTTGGGGAATTTGACTGGTGACCCTTACACTCTTCACACCAATGTTTTTACCAATGGCGTGGGCAAGAGAGAGCAGCAATTCCACCTCTGGTTTGATCCCACTACCGATTTCCACACCTACTCAATCCTCTGGAACCCGCAGCGGATCATGTATGTCTTATCAACCAATCATGTTTTTAAAATTTGAAAATTTTATTTTGTTAATTTCGGTTAAATTGGGGTTTGTTTCAGCTTCAAGGTGGATGGCACGCCCGTCAGGGAGTTCAAGAACGAGGAATCCTCGGGCGTTATGTTCCCAACGAATCAGCCGATGAGGCTCTACTCCAGCCTCTGGAACGCCGACGACTGGGCCACCAGAGGAGGGCTCGTCAAGACGGATTGGTCCAAAGCTCCCTTCACCGCCGCCTACCGGAACTTCAACGCCGACGCCTGCGTTTGGTCCGCCGGCGGCTCCTCCTGCTCCTCGGGCGCCCCTTCTGGCCACGGATGGCTCTCCGAAAACCTGGACAACACTCAACAACAGAGGCTGAGGTGGGTGCAGAAAAATTTCATGATTTACAATTACTGCACGGACGCCAAGAGGTTCCCGCAAGGCTTCCCTCCCGAGTGCTCTGTCAACGCCGGATCC

mRNA sequence

AAATATCCAATGCCTCCTCCTGCTTTGTCACTCCTGCTCTTTCTGACTCTCTCTGTTTCCTCTGTTTTTGCTGCCAACTTCAACCAGGATGTTGAGCTCATTTGGGGCAATGAACGTGCCAATATCCTTGACGATGGCCACCTCCTCACTCTCTCCTTGGACCGAGCCTCTGGCTCCGGCTTCCAGTCCAAAAATGTTTACCTTTTTGGTAAAATTGAGATGCAGCTCAAACTTGTCCCTGGAAACTCCGCTGGCACTGTCACCACTTACTATCTTAAATCCGAAGGGTCGACGTGGGATGAGATAGATTTTGAATTCTTGGGGAATTTGACTGGTGACCCTTACACTCTTCACACCAATGTTTTTACCAATGGCGTGGGCAAGAGAGAGCAGCAATTCCACCTCTGGTTTGATCCCACTACCGATTTCCACACCTACTCAATCCTCTGGAACCCGCAGCGGATCATCTTCAAGGTGGATGGCACGCCCGTCAGGGAGTTCAAGAACGAGGAATCCTCGGGCGTTATGTTCCCAACGAATCAGCCGATGAGGCTCTACTCCAGCCTCTGGAACGCCGACGACTGGGCCACCAGAGGAGGGCTCGTCAAGACGGATTGGTCCAAAGCTCCCTTCACCGCCGCCTACCGGAACTTCAACGCCGACGCCTGCGTTTGGTCCGCCGGCGGCTCCTCCTGCTCCTCGGGCGCCCCTTCTGGCCACGGATGGCTCTCCGAAAACCTGGACAACACTCAACAACAGAGGCTGAGGTGGGTGCAGAAAAATTTCATGATTTACAATTACTGCACGGACGCCAAGAGGTTCCCGCAAGGCTTCCCTCCCGAGTGCTCTGTCAACGCCGGATCC

Coding sequence (CDS)

AAATATCCAATGCCTCCTCCTGCTTTGTCACTCCTGCTCTTTCTGACTCTCTCTGTTTCCTCTGTTTTTGCTGCCAACTTCAACCAGGATGTTGAGCTCATTTGGGGCAATGAACGTGCCAATATCCTTGACGATGGCCACCTCCTCACTCTCTCCTTGGACCGAGCCTCTGGCTCCGGCTTCCAGTCCAAAAATGTTTACCTTTTTGGTAAAATTGAGATGCAGCTCAAACTTGTCCCTGGAAACTCCGCTGGCACTGTCACCACTTACTATCTTAAATCCGAAGGGTCGACGTGGGATGAGATAGATTTTGAATTCTTGGGGAATTTGACTGGTGACCCTTACACTCTTCACACCAATGTTTTTACCAATGGCGTGGGCAAGAGAGAGCAGCAATTCCACCTCTGGTTTGATCCCACTACCGATTTCCACACCTACTCAATCCTCTGGAACCCGCAGCGGATCATCTTCAAGGTGGATGGCACGCCCGTCAGGGAGTTCAAGAACGAGGAATCCTCGGGCGTTATGTTCCCAACGAATCAGCCGATGAGGCTCTACTCCAGCCTCTGGAACGCCGACGACTGGGCCACCAGAGGAGGGCTCGTCAAGACGGATTGGTCCAAAGCTCCCTTCACCGCCGCCTACCGGAACTTCAACGCCGACGCCTGCGTTTGGTCCGCCGGCGGCTCCTCCTGCTCCTCGGGCGCCCCTTCTGGCCACGGATGGCTCTCCGAAAACCTGGACAACACTCAACAACAGAGGCTGAGGTGGGTGCAGAAAAATTTCATGATTTACAATTACTGCACGGACGCCAAGAGGTTCCCGCAAGGCTTCCCTCCCGAGTGCTCTGTCAACGCCGGATCC

Protein sequence

KYPMPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSKNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGAPSGHGWLSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS
Homology
BLAST of MS002257 vs. NCBI nr
Match: XP_022135083.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like [Momordica charantia])

HSP 1 Score: 601.7 bits (1550), Expect = 3.4e-168
Identity = 284/285 (99.65%), Postives = 285/285 (100.00%), Query Frame = 0

Query: 4   MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 63
           MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS
Sbjct: 1   MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 60

Query: 64  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 123
           KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT
Sbjct: 61  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 120

Query: 124 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 183
           NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM
Sbjct: 121 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 180

Query: 184 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGAPSGHGWL 243
           RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNAD+CVWSAGGSSCSSGAPSGHGWL
Sbjct: 181 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWL 240

Query: 244 SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS 289
           SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS
Sbjct: 241 SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS 285

BLAST of MS002257 vs. NCBI nr
Match: CCH26406.1 (xyloglucan endotransglycosylase [Cucumis sativus])

HSP 1 Score: 498.0 bits (1281), Expect = 5.3e-137
Identity = 233/282 (82.62%), Postives = 253/282 (89.72%), Query Frame = 0

Query: 5   PPPALSLLLFLT-LSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 64
           PPP  SLLLFL+ LSVSSVF  NF  D E+ WGN+RA IL++G LLTLSLDR SGSGFQS
Sbjct: 4   PPPFSSLLLFLSLLSVSSVFCGNFYNDFEISWGNDRAKILNNGDLLTLSLDRGSGSGFQS 63

Query: 65  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 124
           KNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+T
Sbjct: 64  KNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVYT 123

Query: 125 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 184
            G G REQQFHLWFDPT DFHTYSILWNPQ IIF VDGTP+REFKN ESSGV+FP NQPM
Sbjct: 124 QGKGDREQQFHLWFDPTYDFHTYSILWNPQGIIFSVDGTPIREFKNHESSGVLFPKNQPM 183

Query: 185 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGA-PSGHGW 244
           RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNA+ CVWS G SSCSSG    G GW
Sbjct: 184 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNANVCVWSGGASSCSSGTNVGGRGW 243

Query: 245 LSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 285
           LSENLD T+QQR++WVQ+N+MIYNYCTDAKRFPQG+PPECS+
Sbjct: 244 LSENLDITRQQRMKWVQRNYMIYNYCTDAKRFPQGYPPECSL 285

BLAST of MS002257 vs. NCBI nr
Match: XP_004146309.1 (xyloglucan endotransglucosylase/hydrolase protein 22 [Cucumis sativus] >KGN65439.1 hypothetical protein Csa_019769 [Cucumis sativus])

HSP 1 Score: 495.4 bits (1274), Expect = 3.4e-136
Identity = 232/282 (82.27%), Postives = 252/282 (89.36%), Query Frame = 0

Query: 5   PPPALSLLLFLT-LSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 64
           P P  SLLLFL+ LSVSSVF  NF  D E+ WGN+RA IL++G LLTLSLDR SGSGFQS
Sbjct: 4   PSPFSSLLLFLSLLSVSSVFCGNFYNDFEITWGNDRAKILNNGDLLTLSLDRGSGSGFQS 63

Query: 65  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 124
           KNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+T
Sbjct: 64  KNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVYT 123

Query: 125 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 184
            G G REQQFHLWFDPT DFHTYSILWNPQ IIF VDGTP+REFKN ESSGV+FP NQPM
Sbjct: 124 QGKGDREQQFHLWFDPTYDFHTYSILWNPQGIIFSVDGTPIREFKNHESSGVLFPKNQPM 183

Query: 185 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGA-PSGHGW 244
           RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNA+ CVWS G SSCSSG    G GW
Sbjct: 184 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNANVCVWSGGASSCSSGTNVGGRGW 243

Query: 245 LSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 285
           LSENLD T+QQR++WVQ+N+MIYNYCTDAKRFPQG+PPECS+
Sbjct: 244 LSENLDITRQQRMKWVQRNYMIYNYCTDAKRFPQGYPPECSL 285

BLAST of MS002257 vs. NCBI nr
Match: XP_038880531.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like [Benincasa hispida])

HSP 1 Score: 493.4 bits (1269), Expect = 1.3e-135
Identity = 231/282 (81.91%), Postives = 253/282 (89.72%), Query Frame = 0

Query: 5   PPPALSLLLFLTL-SVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 64
           PPP  SLL FL+L SVSSVF  NF QD E+ WGN RANIL++G LLTLSLDR SGSGFQS
Sbjct: 3   PPPFSSLLFFLSLFSVSSVFCGNFYQDFEITWGNGRANILNNGELLTLSLDRGSGSGFQS 62

Query: 65  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 124
           KNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+T
Sbjct: 63  KNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVYT 122

Query: 125 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 184
            G G REQQFHLWFDPT+DFHTYSILWNPQ IIF VDGTP+REFKN ESSGV+FP  QPM
Sbjct: 123 QGKGDREQQFHLWFDPTSDFHTYSILWNPQGIIFSVDGTPIREFKNHESSGVLFPKKQPM 182

Query: 185 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGA-PSGHGW 244
           RLYSSLWNADDWATRGGLVKTDWS APFTA+YRNFNAD CVWSAG SSCSS A   G GW
Sbjct: 183 RLYSSLWNADDWATRGGLVKTDWSHAPFTASYRNFNADVCVWSAGVSSCSSDANVGGKGW 242

Query: 245 LSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 285
           LSENLD T+QQR++WVQ+N+MIYNYC+DAKRFPQG+PPEC++
Sbjct: 243 LSENLDITRQQRMKWVQRNYMIYNYCSDAKRFPQGYPPECAL 284

BLAST of MS002257 vs. NCBI nr
Match: NP_001284442.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like precursor [Cucumis melo] >ABI94062.1 xyloglucan endotransglucosylase/hydrolase 2 [Cucumis melo])

HSP 1 Score: 492.7 bits (1267), Expect = 2.2e-135
Identity = 230/283 (81.27%), Postives = 255/283 (90.11%), Query Frame = 0

Query: 5   PPPAL-SLLLFLT-LSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQ 64
           PPP+  SLLLFL+ LSVSSVF +NF  D E+ WGN+RA IL++G LLTLSLDR SGSGFQ
Sbjct: 3   PPPSFSSLLLFLSLLSVSSVFCSNFYNDFEITWGNDRAKILNNGDLLTLSLDRGSGSGFQ 62

Query: 65  SKNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVF 124
           SKNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+
Sbjct: 63  SKNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVY 122

Query: 125 TNGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQP 184
           T G G REQQFHLWFDPT+DFHTYSILWNPQ IIF VDGTP+R+FKN ESSGV+FP  QP
Sbjct: 123 TQGKGDREQQFHLWFDPTSDFHTYSILWNPQGIIFSVDGTPIRKFKNHESSGVLFPKKQP 182

Query: 185 MRLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGA-PSGHG 244
           MRLYSSLWNADDWATRGGLVKTDWSKAPFTA+YRNFNAD CVWS G SSCSSG    G G
Sbjct: 183 MRLYSSLWNADDWATRGGLVKTDWSKAPFTASYRNFNADVCVWSGGVSSCSSGGNVGGRG 242

Query: 245 WLSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 285
           WLSENLD T+QQR++WVQ+N+MIYNYCTDAKRFPQG+PPEC++
Sbjct: 243 WLSENLDITRQQRMKWVQRNYMIYNYCTDAKRFPQGYPPECAI 285

BLAST of MS002257 vs. ExPASy Swiss-Prot
Match: Q38857 (Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=3702 GN=XTH22 PE=1 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 1.6e-120
Identity = 198/275 (72.00%), Postives = 231/275 (84.00%), Query Frame = 0

Query: 11  LLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSKNVYLFG 70
           L LFL+L ++S  +ANF +DVE+ WG+ R  I ++G LLTLSLD++SGSGFQSKN YLFG
Sbjct: 7   LPLFLSLIITSSVSANFQRDVEITWGDGRGQIKNNGELLTLSLDKSSGSGFQSKNEYLFG 66

Query: 71  KIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGVGKRE 130
           K+ MQ+KLVPGNSAGTVTT YLKS G+TWDEIDFEFLGN +G+PYTLHTNV+T G G +E
Sbjct: 67  KVSMQMKLVPGNSAGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQGKGDKE 126

Query: 131 QQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLYSSLW 190
           QQF LWFDPT +FHTY+ILWNPQRIIF VDGTP+REFKN ES G +FP N+PMR+YSSLW
Sbjct: 127 QQFKLWFDPTANFHTYTILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMRMYSSLW 186

Query: 191 NADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGAPSG---HGWLSENL 250
           NADDWATRGGLVKTDWSKAPFTA+YR F  +ACVWS G SSC + +  G     WLS+ L
Sbjct: 187 NADDWATRGGLVKTDWSKAPFTASYRGFQQEACVWSNGKSSCPNASKQGTTTGSWLSQEL 246

Query: 251 DNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPEC 283
           D+T QQR+RWVQ+N+MIYNYCTDAKRFPQG P EC
Sbjct: 247 DSTAQQRMRWVQRNYMIYNYCTDAKRFPQGLPKEC 281

BLAST of MS002257 vs. ExPASy Swiss-Prot
Match: Q38910 (Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis thaliana OX=3702 GN=XTH23 PE=2 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 6.7e-119
Identity = 203/274 (74.09%), Postives = 227/274 (82.85%), Query Frame = 0

Query: 11  LLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSKNVYLFG 70
           L  F+  SVS    ANF +DVE+ WG+ R  I ++G LLTLSLD+ASGSGFQSKN YLFG
Sbjct: 14  LASFMICSVS----ANFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEYLFG 73

Query: 71  KIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGVGKRE 130
           KI+MQ+KLV GNSAGTVT YYLKS GSTWDEIDFEFLGNL+GDPYTLHTNVFT G G RE
Sbjct: 74  KIDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKGDRE 133

Query: 131 QQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLYSSLW 190
           QQF LWFDPT+DFHTYSILWNPQRIIF VDGTP+REFKN ES G +FP NQPMR+YSSLW
Sbjct: 134 QQFKLWFDPTSDFHTYSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYSSLW 193

Query: 191 NADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCS--SGAPSGHGWLSENLD 250
           NA++WATRGGLVKTDWSKAPFTA+YR FN +ACV   G SSC   SG  S   WLS+ LD
Sbjct: 194 NAEEWATRGGLVKTDWSKAPFTASYRGFNEEACVVINGQSSCPNVSGQGSTGSWLSQELD 253

Query: 251 NTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPEC 283
           +T Q+++RWVQ N+MIYNYCTDAKRFPQG P EC
Sbjct: 254 STGQEQMRWVQNNYMIYNYCTDAKRFPQGLPREC 283

BLAST of MS002257 vs. ExPASy Swiss-Prot
Match: Q38907 (Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis thaliana OX=3702 GN=XTH25 PE=2 SV=2)

HSP 1 Score: 417.9 bits (1073), Expect = 9.1e-116
Identity = 195/278 (70.14%), Postives = 223/278 (80.22%), Query Frame = 0

Query: 9   LSLLLFLTLSV----SSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSK 68
           LSLL  LT+S     S VFA  F+ + ++ WG+ R  +L++G LLTLSLDRASGSGFQ+K
Sbjct: 8   LSLLFTLTVSTTTLFSPVFAGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTK 67

Query: 69  NVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTN 128
             YLFGKI+MQLKLVPGNSAGTVT YYLKS+G TWDEIDFEFLGNLTGDPYT+HTNV+T 
Sbjct: 68  KEYLFGKIDMQLKLVPGNSAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYTQ 127

Query: 129 GVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMR 188
           G G REQQFHLWFDPT DFHTYS+LWNP  I+F VD  PVREFKN +  G+ +P  QPMR
Sbjct: 128 GKGDREQQFHLWFDPTADFHTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPKLQPMR 187

Query: 189 LYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGAPSGHGWLS 248
           LYSSLWNAD WATRGGLVKTDWSKAPFTA+YRNF ADACV S G SSC +G+P    W S
Sbjct: 188 LYSSLWNADQWATRGGLVKTDWSKAPFTASYRNFRADACVSSGGRSSCPAGSPR---WFS 247

Query: 249 ENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPEC 283
           + LD T + ++R VQ+ +MIYNYCTD KRFPQGFP EC
Sbjct: 248 QRLDLTAEDKMRVVQRKYMIYNYCTDTKRFPQGFPKEC 282

BLAST of MS002257 vs. ExPASy Swiss-Prot
Match: P24806 (Xyloglucan endotransglucosylase/hydrolase protein 24 OS=Arabidopsis thaliana OX=3702 GN=XTH24 PE=1 SV=2)

HSP 1 Score: 406.4 bits (1043), Expect = 2.7e-112
Identity = 187/283 (66.08%), Postives = 221/283 (78.09%), Query Frame = 0

Query: 4   MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 63
           M P  +     L ++  SV AA+FN DV + WGN R  IL++G LLTLSLD++SGSGFQS
Sbjct: 1   MSPFKIFFFTTLLVAAFSVSAADFNTDVNVAWGNGRGKILNNGQLLTLSLDKSSGSGFQS 60

Query: 64  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 123
           K  YLFGKI+MQ+KLVPGNSAGTVTT+YLKSEGSTWDEIDFEFLGN++GDPYTLHTNV+T
Sbjct: 61  KTEYLFGKIDMQIKLVPGNSAGTVTTFYLKSEGSTWDEIDFEFLGNMSGDPYTLHTNVYT 120

Query: 124 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 183
            G G +EQQFHLWFDPT +FHTYSILWNPQRII  VD TP+REFKN ES GV+FP N+PM
Sbjct: 121 QGKGDKEQQFHLWFDPTANFHTYSILWNPQRIILTVDDTPIREFKNYESLGVLFPKNKPM 180

Query: 184 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGAPSGHGWL 243
           R+Y+SLWNADDWATRGGLVKTDWSKAPF A+YRN   D              +     W 
Sbjct: 181 RMYASLWNADDWATRGGLVKTDWSKAPFMASYRNIKID--------------SKPNSNWY 240

Query: 244 SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNA 287
           ++ +D+T Q RL+WVQKN+MIYNYCTD +RFPQG P EC+ ++
Sbjct: 241 TQEMDSTSQARLKWVQKNYMIYNYCTDHRRFPQGAPKECTTSS 269

BLAST of MS002257 vs. ExPASy Swiss-Prot
Match: Q9FI31 (Xyloglucan endotransglucosylase/hydrolase protein 20 OS=Arabidopsis thaliana OX=3702 GN=XTH20 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 9.8e-110
Identity = 177/277 (63.90%), Postives = 220/277 (79.42%), Query Frame = 0

Query: 8   ALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDD-GHLLTLSLDRASGSGFQSKNV 67
           A  ++         V+A +F++DV++ WG+ R  ILD+ G+LL+LSLD+ SGSGFQS   
Sbjct: 10  AFLIIFLFAAQYERVYAGSFHKDVQIHWGDGRGKILDNVGNLLSLSLDKFSGSGFQSHQE 69

Query: 68  YLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGV 127
           +L+GK+E+Q+KLVPGNSAGTVTT+YLKS G+TWDEIDFEFLGN++G PYTLHTNV+T G 
Sbjct: 70  FLYGKVEVQMKLVPGNSAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGT 129

Query: 128 GKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLY 187
           G +EQQFHLWFDPT DFHTY I+WNPQR+IF +DG P+REFKN E+ GV FP +QPMRLY
Sbjct: 130 GDKEQQFHLWFDPTVDFHTYCIIWNPQRVIFTIDGIPIREFKNSEALGVPFPKHQPMRLY 189

Query: 188 SSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGAPSGHGWLSEN 247
           +SLW A+ WATRGGL KTDWSKAPFTA YRN+N DACVWS G SSCS+ +     W ++ 
Sbjct: 190 ASLWEAEHWATRGGLEKTDWSKAPFTAFYRNYNVDACVWSNGKSSCSANS----SWFTQV 249

Query: 248 LDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECS 284
           LD   + R++W Q+ +M+YNYCTD KRFPQG PPECS
Sbjct: 250 LDFKGKNRVKWAQRKYMVYNYCTDKKRFPQGAPPECS 282

BLAST of MS002257 vs. ExPASy TrEMBL
Match: A0A6J1C3T7 (Xyloglucan endotransglucosylase/hydrolase OS=Momordica charantia OX=3673 GN=LOC111007155 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.6e-168
Identity = 284/285 (99.65%), Postives = 285/285 (100.00%), Query Frame = 0

Query: 4   MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 63
           MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS
Sbjct: 1   MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 60

Query: 64  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 123
           KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT
Sbjct: 61  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 120

Query: 124 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 183
           NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM
Sbjct: 121 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 180

Query: 184 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGAPSGHGWL 243
           RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNAD+CVWSAGGSSCSSGAPSGHGWL
Sbjct: 181 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADSCVWSAGGSSCSSGAPSGHGWL 240

Query: 244 SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS 289
           SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS
Sbjct: 241 SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS 285

BLAST of MS002257 vs. ExPASy TrEMBL
Match: N0DX33 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth6 PE=3 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.6e-137
Identity = 233/282 (82.62%), Postives = 253/282 (89.72%), Query Frame = 0

Query: 5   PPPALSLLLFLT-LSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 64
           PPP  SLLLFL+ LSVSSVF  NF  D E+ WGN+RA IL++G LLTLSLDR SGSGFQS
Sbjct: 4   PPPFSSLLLFLSLLSVSSVFCGNFYNDFEISWGNDRAKILNNGDLLTLSLDRGSGSGFQS 63

Query: 65  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 124
           KNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+T
Sbjct: 64  KNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVYT 123

Query: 125 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 184
            G G REQQFHLWFDPT DFHTYSILWNPQ IIF VDGTP+REFKN ESSGV+FP NQPM
Sbjct: 124 QGKGDREQQFHLWFDPTYDFHTYSILWNPQGIIFSVDGTPIREFKNHESSGVLFPKNQPM 183

Query: 185 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGA-PSGHGW 244
           RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNA+ CVWS G SSCSSG    G GW
Sbjct: 184 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNANVCVWSGGASSCSSGTNVGGRGW 243

Query: 245 LSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 285
           LSENLD T+QQR++WVQ+N+MIYNYCTDAKRFPQG+PPECS+
Sbjct: 244 LSENLDITRQQRMKWVQRNYMIYNYCTDAKRFPQGYPPECSL 285

BLAST of MS002257 vs. ExPASy TrEMBL
Match: A0A0A0LWT1 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G422480 PE=3 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.7e-136
Identity = 232/282 (82.27%), Postives = 252/282 (89.36%), Query Frame = 0

Query: 5   PPPALSLLLFLT-LSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 64
           P P  SLLLFL+ LSVSSVF  NF  D E+ WGN+RA IL++G LLTLSLDR SGSGFQS
Sbjct: 4   PSPFSSLLLFLSLLSVSSVFCGNFYNDFEITWGNDRAKILNNGDLLTLSLDRGSGSGFQS 63

Query: 65  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 124
           KNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+T
Sbjct: 64  KNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVYT 123

Query: 125 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 184
            G G REQQFHLWFDPT DFHTYSILWNPQ IIF VDGTP+REFKN ESSGV+FP NQPM
Sbjct: 124 QGKGDREQQFHLWFDPTYDFHTYSILWNPQGIIFSVDGTPIREFKNHESSGVLFPKNQPM 183

Query: 185 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGA-PSGHGW 244
           RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNA+ CVWS G SSCSSG    G GW
Sbjct: 184 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNANVCVWSGGASSCSSGTNVGGRGW 243

Query: 245 LSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 285
           LSENLD T+QQR++WVQ+N+MIYNYCTDAKRFPQG+PPECS+
Sbjct: 244 LSENLDITRQQRMKWVQRNYMIYNYCTDAKRFPQGYPPECSL 285

BLAST of MS002257 vs. ExPASy TrEMBL
Match: Q06BI5 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=XTH2 PE=2 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.1e-135
Identity = 230/283 (81.27%), Postives = 255/283 (90.11%), Query Frame = 0

Query: 5   PPPAL-SLLLFLT-LSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQ 64
           PPP+  SLLLFL+ LSVSSVF +NF  D E+ WGN+RA IL++G LLTLSLDR SGSGFQ
Sbjct: 3   PPPSFSSLLLFLSLLSVSSVFCSNFYNDFEITWGNDRAKILNNGDLLTLSLDRGSGSGFQ 62

Query: 65  SKNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVF 124
           SKNVYL+GKI+MQLKLVPGNSAGTVTTYYLKS+GSTWDEIDFEFLGNL+GDPYTLHTNV+
Sbjct: 63  SKNVYLYGKIDMQLKLVPGNSAGTVTTYYLKSQGSTWDEIDFEFLGNLSGDPYTLHTNVY 122

Query: 125 TNGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQP 184
           T G G REQQFHLWFDPT+DFHTYSILWNPQ IIF VDGTP+R+FKN ESSGV+FP  QP
Sbjct: 123 TQGKGDREQQFHLWFDPTSDFHTYSILWNPQGIIFSVDGTPIRKFKNHESSGVLFPKKQP 182

Query: 185 MRLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGA-PSGHG 244
           MRLYSSLWNADDWATRGGLVKTDWSKAPFTA+YRNFNAD CVWS G SSCSSG    G G
Sbjct: 183 MRLYSSLWNADDWATRGGLVKTDWSKAPFTASYRNFNADVCVWSGGVSSCSSGGNVGGRG 242

Query: 245 WLSENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSV 285
           WLSENLD T+QQR++WVQ+N+MIYNYCTDAKRFPQG+PPEC++
Sbjct: 243 WLSENLDITRQQRMKWVQRNYMIYNYCTDAKRFPQGYPPECAI 285

BLAST of MS002257 vs. ExPASy TrEMBL
Match: A0A6J1KN18 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111497152 PE=3 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 9.1e-135
Identity = 228/280 (81.43%), Postives = 250/280 (89.29%), Query Frame = 0

Query: 10  SLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSKNVYLF 69
           SLLLF    VSSVF  NF QD E+ WGN+RANILD+G LLTLSLDR SGSGF+S NVYLF
Sbjct: 15  SLLLF----VSSVFGGNFYQDFEITWGNDRANILDNGDLLTLSLDRDSGSGFRSNNVYLF 74

Query: 70  GKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGVGKR 129
            KI+MQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNV+T G G R
Sbjct: 75  AKIDMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVYTQGKGDR 134

Query: 130 EQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLYSSL 189
           EQQFHLWFDPT DFHTYSILWNP+ IIF VDGTP+REFKN+ESSGV+FPT QPMRLYSSL
Sbjct: 135 EQQFHLWFDPTADFHTYSILWNPEGIIFSVDGTPIREFKNQESSGVLFPTKQPMRLYSSL 194

Query: 190 WNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGAPS-GHGWLSENLD 249
           WNADDWATRGGLVKTDWS+APF ++YRNF AD CVWS G SSCSSGA S G GWLSENLD
Sbjct: 195 WNADDWATRGGLVKTDWSQAPFVSSYRNFKADVCVWSEGVSSCSSGAHSAGGGWLSENLD 254

Query: 250 NTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNAGS 289
           NT+QQRL+WVQ+N+MIYNYCTDA+RFPQG+PPEC++N  +
Sbjct: 255 NTRQQRLKWVQRNYMIYNYCTDAERFPQGYPPECTLNTAA 290

BLAST of MS002257 vs. TAIR 10
Match: AT5G57560.1 (Xyloglucan endotransglucosylase/hydrolase family protein )

HSP 1 Score: 433.7 bits (1114), Expect = 1.1e-121
Identity = 198/275 (72.00%), Postives = 231/275 (84.00%), Query Frame = 0

Query: 11  LLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSKNVYLFG 70
           L LFL+L ++S  +ANF +DVE+ WG+ R  I ++G LLTLSLD++SGSGFQSKN YLFG
Sbjct: 7   LPLFLSLIITSSVSANFQRDVEITWGDGRGQIKNNGELLTLSLDKSSGSGFQSKNEYLFG 66

Query: 71  KIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGVGKRE 130
           K+ MQ+KLVPGNSAGTVTT YLKS G+TWDEIDFEFLGN +G+PYTLHTNV+T G G +E
Sbjct: 67  KVSMQMKLVPGNSAGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQGKGDKE 126

Query: 131 QQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLYSSLW 190
           QQF LWFDPT +FHTY+ILWNPQRIIF VDGTP+REFKN ES G +FP N+PMR+YSSLW
Sbjct: 127 QQFKLWFDPTANFHTYTILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMRMYSSLW 186

Query: 191 NADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGAPSG---HGWLSENL 250
           NADDWATRGGLVKTDWSKAPFTA+YR F  +ACVWS G SSC + +  G     WLS+ L
Sbjct: 187 NADDWATRGGLVKTDWSKAPFTASYRGFQQEACVWSNGKSSCPNASKQGTTTGSWLSQEL 246

Query: 251 DNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPEC 283
           D+T QQR+RWVQ+N+MIYNYCTDAKRFPQG P EC
Sbjct: 247 DSTAQQRMRWVQRNYMIYNYCTDAKRFPQGLPKEC 281

BLAST of MS002257 vs. TAIR 10
Match: AT4G25810.1 (xyloglucan endotransglycosylase 6 )

HSP 1 Score: 428.3 bits (1100), Expect = 4.8e-120
Identity = 203/274 (74.09%), Postives = 227/274 (82.85%), Query Frame = 0

Query: 11  LLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSKNVYLFG 70
           L  F+  SVS    ANF +DVE+ WG+ R  I ++G LLTLSLD+ASGSGFQSKN YLFG
Sbjct: 14  LASFMICSVS----ANFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEYLFG 73

Query: 71  KIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGVGKRE 130
           KI+MQ+KLV GNSAGTVT YYLKS GSTWDEIDFEFLGNL+GDPYTLHTNVFT G G RE
Sbjct: 74  KIDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKGDRE 133

Query: 131 QQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLYSSLW 190
           QQF LWFDPT+DFHTYSILWNPQRIIF VDGTP+REFKN ES G +FP NQPMR+YSSLW
Sbjct: 134 QQFKLWFDPTSDFHTYSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYSSLW 193

Query: 191 NADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCS--SGAPSGHGWLSENLD 250
           NA++WATRGGLVKTDWSKAPFTA+YR FN +ACV   G SSC   SG  S   WLS+ LD
Sbjct: 194 NAEEWATRGGLVKTDWSKAPFTASYRGFNEEACVVINGQSSCPNVSGQGSTGSWLSQELD 253

Query: 251 NTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPEC 283
           +T Q+++RWVQ N+MIYNYCTDAKRFPQG P EC
Sbjct: 254 STGQEQMRWVQNNYMIYNYCTDAKRFPQGLPREC 283

BLAST of MS002257 vs. TAIR 10
Match: AT5G57550.1 (xyloglucan endotransglucosylase/hydrolase 25 )

HSP 1 Score: 417.9 bits (1073), Expect = 6.5e-117
Identity = 195/278 (70.14%), Postives = 223/278 (80.22%), Query Frame = 0

Query: 9   LSLLLFLTLSV----SSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQSK 68
           LSLL  LT+S     S VFA  F+ + ++ WG+ R  +L++G LLTLSLDRASGSGFQ+K
Sbjct: 8   LSLLFTLTVSTTTLFSPVFAGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTK 67

Query: 69  NVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTN 128
             YLFGKI+MQLKLVPGNSAGTVT YYLKS+G TWDEIDFEFLGNLTGDPYT+HTNV+T 
Sbjct: 68  KEYLFGKIDMQLKLVPGNSAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYTQ 127

Query: 129 GVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMR 188
           G G REQQFHLWFDPT DFHTYS+LWNP  I+F VD  PVREFKN +  G+ +P  QPMR
Sbjct: 128 GKGDREQQFHLWFDPTADFHTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPKLQPMR 187

Query: 189 LYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGAPSGHGWLS 248
           LYSSLWNAD WATRGGLVKTDWSKAPFTA+YRNF ADACV S G SSC +G+P    W S
Sbjct: 188 LYSSLWNADQWATRGGLVKTDWSKAPFTASYRNFRADACVSSGGRSSCPAGSPR---WFS 247

Query: 249 ENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPEC 283
           + LD T + ++R VQ+ +MIYNYCTD KRFPQGFP EC
Sbjct: 248 QRLDLTAEDKMRVVQRKYMIYNYCTDTKRFPQGFPKEC 282

BLAST of MS002257 vs. TAIR 10
Match: AT4G30270.1 (xyloglucan endotransglucosylase/hydrolase 24 )

HSP 1 Score: 406.4 bits (1043), Expect = 2.0e-113
Identity = 187/283 (66.08%), Postives = 221/283 (78.09%), Query Frame = 0

Query: 4   MPPPALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDDGHLLTLSLDRASGSGFQS 63
           M P  +     L ++  SV AA+FN DV + WGN R  IL++G LLTLSLD++SGSGFQS
Sbjct: 1   MSPFKIFFFTTLLVAAFSVSAADFNTDVNVAWGNGRGKILNNGQLLTLSLDKSSGSGFQS 60

Query: 64  KNVYLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFT 123
           K  YLFGKI+MQ+KLVPGNSAGTVTT+YLKSEGSTWDEIDFEFLGN++GDPYTLHTNV+T
Sbjct: 61  KTEYLFGKIDMQIKLVPGNSAGTVTTFYLKSEGSTWDEIDFEFLGNMSGDPYTLHTNVYT 120

Query: 124 NGVGKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPM 183
            G G +EQQFHLWFDPT +FHTYSILWNPQRII  VD TP+REFKN ES GV+FP N+PM
Sbjct: 121 QGKGDKEQQFHLWFDPTANFHTYSILWNPQRIILTVDDTPIREFKNYESLGVLFPKNKPM 180

Query: 184 RLYSSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGAPSGHGWL 243
           R+Y+SLWNADDWATRGGLVKTDWSKAPF A+YRN   D              +     W 
Sbjct: 181 RMYASLWNADDWATRGGLVKTDWSKAPFMASYRNIKID--------------SKPNSNWY 240

Query: 244 SENLDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECSVNA 287
           ++ +D+T Q RL+WVQKN+MIYNYCTD +RFPQG P EC+ ++
Sbjct: 241 TQEMDSTSQARLKWVQKNYMIYNYCTDHRRFPQGAPKECTTSS 269

BLAST of MS002257 vs. TAIR 10
Match: AT5G48070.1 (xyloglucan endotransglucosylase/hydrolase 20 )

HSP 1 Score: 397.9 bits (1021), Expect = 6.9e-111
Identity = 177/277 (63.90%), Postives = 220/277 (79.42%), Query Frame = 0

Query: 8   ALSLLLFLTLSVSSVFAANFNQDVELIWGNERANILDD-GHLLTLSLDRASGSGFQSKNV 67
           A  ++         V+A +F++DV++ WG+ R  ILD+ G+LL+LSLD+ SGSGFQS   
Sbjct: 10  AFLIIFLFAAQYERVYAGSFHKDVQIHWGDGRGKILDNVGNLLSLSLDKFSGSGFQSHQE 69

Query: 68  YLFGKIEMQLKLVPGNSAGTVTTYYLKSEGSTWDEIDFEFLGNLTGDPYTLHTNVFTNGV 127
           +L+GK+E+Q+KLVPGNSAGTVTT+YLKS G+TWDEIDFEFLGN++G PYTLHTNV+T G 
Sbjct: 70  FLYGKVEVQMKLVPGNSAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGT 129

Query: 128 GKREQQFHLWFDPTTDFHTYSILWNPQRIIFKVDGTPVREFKNEESSGVMFPTNQPMRLY 187
           G +EQQFHLWFDPT DFHTY I+WNPQR+IF +DG P+REFKN E+ GV FP +QPMRLY
Sbjct: 130 GDKEQQFHLWFDPTVDFHTYCIIWNPQRVIFTIDGIPIREFKNSEALGVPFPKHQPMRLY 189

Query: 188 SSLWNADDWATRGGLVKTDWSKAPFTAAYRNFNADACVWSAGGSSCSSGAPSGHGWLSEN 247
           +SLW A+ WATRGGL KTDWSKAPFTA YRN+N DACVWS G SSCS+ +     W ++ 
Sbjct: 190 ASLWEAEHWATRGGLEKTDWSKAPFTAFYRNYNVDACVWSNGKSSCSANS----SWFTQV 249

Query: 248 LDNTQQQRLRWVQKNFMIYNYCTDAKRFPQGFPPECS 284
           LD   + R++W Q+ +M+YNYCTD KRFPQG PPECS
Sbjct: 250 LDFKGKNRVKWAQRKYMVYNYCTDKKRFPQGAPPECS 282

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135083.13.4e-16899.65xyloglucan endotransglucosylase/hydrolase protein 22-like [Momordica charantia][more]
CCH26406.15.3e-13782.62xyloglucan endotransglycosylase [Cucumis sativus][more]
XP_004146309.13.4e-13682.27xyloglucan endotransglucosylase/hydrolase protein 22 [Cucumis sativus] >KGN65439... [more]
XP_038880531.11.3e-13581.91xyloglucan endotransglucosylase/hydrolase protein 22-like [Benincasa hispida][more]
NP_001284442.12.2e-13581.27xyloglucan endotransglucosylase/hydrolase protein 22-like precursor [Cucumis mel... [more]
Match NameE-valueIdentityDescription
Q388571.6e-12072.00Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=... [more]
Q389106.7e-11974.09Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis tha... [more]
Q389079.1e-11670.14Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis tha... [more]
P248062.7e-11266.08Xyloglucan endotransglucosylase/hydrolase protein 24 OS=Arabidopsis thaliana OX=... [more]
Q9FI319.8e-11063.90Xyloglucan endotransglucosylase/hydrolase protein 20 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1C3T71.6e-16899.65Xyloglucan endotransglucosylase/hydrolase OS=Momordica charantia OX=3673 GN=LOC1... [more]
N0DX332.6e-13782.62Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth6 PE=... [more]
A0A0A0LWT11.7e-13682.27Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G42... [more]
Q06BI51.1e-13581.27Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo OX=3656 GN=XTH2 PE=2 S... [more]
A0A6J1KN189.1e-13581.43Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT5G57560.11.1e-12172.00Xyloglucan endotransglucosylase/hydrolase family protein [more]
AT4G25810.14.8e-12074.09xyloglucan endotransglycosylase 6 [more]
AT5G57550.16.5e-11770.14xyloglucan endotransglucosylase/hydrolase 25 [more]
AT4G30270.12.0e-11366.08xyloglucan endotransglucosylase/hydrolase 24 [more]
AT5G48070.16.9e-11163.90xyloglucan endotransglucosylase/hydrolase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008264Beta-glucanasePRINTSPR00737GLHYDRLASE16coord: 59..77
score: 25.44
coord: 117..130
score: 46.43
coord: 135..152
score: 46.76
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 28..208
e-value: 1.6E-59
score: 200.3
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 1..215
score: 31.279146
IPR010713Xyloglucan endo-transglycosylase, C-terminalPFAMPF06955XET_Ccoord: 240..282
e-value: 6.2E-22
score: 77.2
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 7..287
e-value: 3.7E-117
score: 388.6
IPR016455Xyloglucan endotransglucosylase/hydrolaseCDDcd02176GH16_XETcoord: 24..282
e-value: 2.21396E-151
score: 421.993
NoneNo IPR availableGENE3D2.60.120.200coord: 15..287
e-value: 2.3E-109
score: 367.0
NoneNo IPR availablePIRSRPIRSR037299-1PIRSR037299-1coord: 44..220
e-value: 2.9E-29
score: 100.2
NoneNo IPR availablePANTHERPTHR31062:SF178XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASEcoord: 10..283
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 10..283
IPR008263Glycoside hydrolase, family 16, active sitePROSITEPS01034GH16_1coord: 101..111
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 27..285

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002257.1MS002257.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006073 cellular glucan metabolic process
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0010411 xyloglucan metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity