MS002230 (gene) Bitter gourd (TR) v1

Overview
NameMS002230
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionVillin-4
Locationscaffold30: 3440724 .. 3448235 (+)
RNA-Seq ExpressionMS002230
SyntenyMS002230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTCTCTATGAGAGATTTGGATCAAGCTTTTCAGGGTGCTGGCCAGAAAGCGTATCCTTATGTAATGTGACTTCAGCAAGTTTATTATGGTTTATAGTCTCTATTTTCTAATCAACCAAGTTTATAAGGTAAAGGTTTTCCTATGATAATTGATAACATTGTCTACATACCAAGTTTACAAGGTTAAAAGAATTAACAAAGATTTCCCAACAATGATCAATAAAATCTATTTCTTGAACTGCTTTATTAGAAAATTTATTACCATTGGATTTGGAAAAATCTATTTTATTTTCCTTGACAATACATAGTGGCCTTGAAGTATGGCGCATCGAAAATTTCCGCCCTGTTCTTGTACCAAAATCATCTCACGGCAAATTTTTCACAGGCGATTCCTATGTAGTCCTGAAGGTAAGTGGTTTTGGTTTGGTCTTTAAAATGTTCTTTTTAATTTCATCTTCCTGGTGCTTTGACAAGATTTGTCTTGATACTGAACGTTTTATTTTAATAGGTATTCTTAATCTAACATATTGTAGAAGATAATTTTATGCTAAATATGGCAGTACCCAATCTTTCTGTATCTGATGCAGTAGATGTGCTTCCTTTATGAAAATGTCAAAATGTTAACTTCTCATATCTTTATTTGGTCTTTAGTTTTTCAATTAGTTGTCACATGATCTTTCTGAGAGTGCATCTAATTTGATAAAGATAATATGCTAAAAGGATGAAGGGAATAGAATATATATTATCCCTCTGTGTTCTATCTTATGAGGAATTGCAATCATTTTGATGAAGCAACTGCAACCTGCTATGACCGCAGCACTAAAAGAAGAGATAACAAACCATTCTAGGTATCTTTAGCGATTTATGTGTATTATTTATTCTTTGCAGACCTCATCCTTAAAAAGTGGTGCTTTACGGCATGATATTCATTATTGGCTTGGTAAGGATACCACTCAGGTAAGCTTCGATCATTCTTCTTCTTCTTCTTCTTCTTCTTCCTATTTTAATTTTTTTTTTTATAAGAAAATCAGAAAATGTCACAATTTTAAGACCTAATGATTCAGTTCTTGTCTGGTAGTTATGTTTTGGTTTTCAAATTTAAATTTTAAGACCTGTCAATTTAGGATGAAGCCGGTGTTGCAGCCATCAAAACCGTAGAATTGGATGCAGCTCTCGGAGGACGTGCTGTTCAGTACCGGGAAGTGCAAGGTCACGAGACAGAAAAGTTCTTATCATATTTCAAACCGTGCATAATACCGCAAGAAGGTGGTGCTTCATCTGGGTTTAAACATGTAGAGGCTGATGAACACCAGATTCGGTTGTATGTTTGCAAAGGAAAACGTGTTGTTCATGTTAAAGAGGCAAGTCTAACATCAGATATGCAAGCTGATTGGGCATTAGCTTCACCTTTTCTTTTTTGTTTTTTTGGTTTGTTAAGTTGTTTACTCTGGTGGCAGGTTGCTTTTGCTCGATCTTCACTCAACCATGATGATATTTTCATTCTTGATACCAAGTCTAAAATATTCCAATTTAATGGTTCCAACTCATCCATTCAAGAGAGGGCCAAAGCATTGGAAGTAGTTCAATACATCAAAGATACCTATCATGATGGAAAATGTGAGATAGCCACCATTGGTAAGTAAATGGAAGTCTAGTAGTCCTACTTCTTTTTTTTTTTTTCCTTCCATAAATAAAAAATTTTAAATAAGAAGTTTAATTTCCTGTGTTTGAAAGCTAATAGTTTCTATAGTTTTATACCACTAATAGTCCAGTCATGTCGTTTGTCATACAGAGGATGGCAAATTGATGGCAGATGTTGAAACTGGGGAATTTTGGGGTTTATTTGGTGGTTTCGCTCCACTTCCAAGGAAAGTTGCCGGTGATGGTGATAAGACGGTTGATTCACATCCAACTAAACTACTGTGGTAATATGTTGCCCATTTGGGTTTTTGAATTCTCATGAGAGATCATGACTTTATCTTGTCACTAGTTTCTTGCTAAATTTTCATTTATCCCTTGAAAGTAATAAATAAATTTTGGTGGAATGTAATTATATCTGCTTCCAAGTTTCATAATTCTTGCTTATCTTAGTGTTTTAATTTGTAGAACTAATCAACTAATTAGCGATCTAGTTCTGATAGTAGTATGTATGTCTTTGAAGTGTTGTGAAGGGTAATACAGAACCTGTTGAAGCTGATTCTTTAACTAGAGAGCTGTTAGAGACAAATAAATGTTACATTATTGATTGCGGGACAGAAGTTTTTATATGGATGGGAAGAACTACCTCTCTTGATGAAAGGAAAAGTGCAAGCAGAGCTGCAGAAGTAATTCCCTAGACCTCCCTAGTTGATTGGTGTAAGTTCATTCGAAGACAGAGATTTTAATACATCTCCTTGTTGTTAATAGGAGTTAGTTAGTGGTCCTGATCGTCCACAATCGCATATAATGCGTGTGATTGAAGCCTTTGAACCAATAATATTCCGAGCAAAGTTTGATTCATGGCCTGAAACAGCTGCTGTAGCTGTGTCAGAGGATGGTAGAGGCAAGGTTGCAGGTTAGTTATTGAAGAAATTCTCTGATTCATAGTTCTTTTAGCATATCTTGTGGTTTTCCTTTTGGGTTCATTTCACCCCTGTTTTTTTCATTGTAATGTTGACACTTCTCTTTCTATTGTGACGTTCTAATGTTTTCTTCTGTTGTTATCTTTTAGCACTTTTAAAACGCCAAGGAGTTAATGTGAAGGGTCTTTTGAAAGCTGAACCTGTAAAAGAGGAACCTCAACCTTACATTGATTGTACAGGAAATCTACAGGTGATGTTTCTAAAACTATCTACAATTGGCTCTTCTTTTTTAAGACAAGTTTCTGCAGTTGCCTACTTTCAGACTTTTCTCTGGGGAATCTAATTCTAGAAAATATTTGACATTTTTTTCTCTTCTCCATAAGGTTCAATGAACCAATCTGCAATGATGCCACAATAAATAAGTATATGGCATAAGAAGAACAGATAATGGGATGTATGAAAGAAGTGAATATTTGCTTTTCAATCAGTTTGAATGTACAAAGGCACACATGATGACTGGATGGCTTGGGAAGGTCATTGGAAACTAACCAGAAATTTTTGATTAATGGGAAGGGGATATGACTTTAAAAGTTTATCCATTTTTGCCAGCTTAGCTCAGCGTTAATTGACATTTGTCTCTGACCAAGGGGTCATGAGTTTGAATCCCCCACCCCTACTCGTTAGCACTTAAAAAAAAGGTTAATCCGTTTTCGTTATGCATTACGATGTGCTTCAACATGAAATAAGTAGAAATTTCTATATTTTTATTTAATGAGAAACAATGTCATTGATATCATGAAATTGCAGAAGGGAGAACCCAATCCAAAGGAGTTACAAAAAACTTCTCCAATCCGATATGAGAGAAGAAAAACTCATAACTCATAACTCCAATTGGATATAGTTTTATTTAATGGAAAATTGGAGAACATTTACACCAAGTATAACCAAGAGAACTATAGTGTCATAAAAAGGATCAGAAAGCTTTTTTTCTTTGAAAATCCTTCGATTTTGTTCTTGCCACGTAAGTCATAACTCCATAAGAAGGCTCTTCTATAGTCTATTCATAATAATCTGCATAAATGATTTACCAATGATGACAACTTCCTAATTTTACAGGTTTGGCGTGTCAGTGGTCAGGAAAAGCTTTTACTTCCCGTCTCTGATCAGTCAAAATTTTACACTGGAGATTGCTATATCTTCCAATATTCATACCCGGGAGAAGACAGGGAGGAGTTCCTTATCGGAACTTGGTTTGGGAAACAGAGTGTTGAGGTACTCAACTTTTTCCATCCCCAATTCCTTATTTACAATCACCTCCTCAATACCCTTGTGTGAACTAATAAAAACGGACCAGATAAAACTAGGAATAGTAATGTTACGTTTAATTTACGCGTTTGAATCCTCAAAATATTTGAATATTATCATTGGAAAGCTGTTAAAGGTAACATTTTCCATAGGAAATTTTGTAATCTACATTATTTTTTGTGGCATGTCTGGGTTTTATTTATAAGTGCATTTCCTTTTCAAATAGGAAGAAAGAACATCTGCTTTATCAATGGCGAGGAAGATGGTGGAATCACTGAAGTTCCTGCCAGTCCAGGTAGCTTCCACGATTTATATTTTCTGCTTAATAAAAAACATATTTACTCTAAATCCTTTGCTGTCTCTTATTGAGGTATCATCTTTGTTCTGCATTTCTAGGCTCGTATTTATGAAGGATATGAACCAATCCAGTTCTATTCAATCTTTCAAAGCTTTATCGTTTATAAGGTATGTGTAGGTAACTGAATGTAAATGCCTATAATTATACTATATTACATGCTTGCTTCTTGAGATGCTACAAAAGATTTGTATGTTGAATTATTCCCTGAAGTTAATAGATACAAATTTTGCAGGGTGGGTTGAGTGATGGATACAAAAACTACATTACAGAGAATGAAATTCCAGATGTGACAAATGTAGAAGACGGCGTCGCATTGTTTCGAATCCAGGGCTCTGGCCCTGATAATATGCAAGCAATTCAAGTCAAAGCAGTACTTTGCTCTTGTCTCATGGTCTTGCAATTTCACCCATGCATATTTCAAGATAATTTACGTGTTGCTATTTTTTCTTCTTTTGTTCCATCAGGTCGGATCCTCTTTGAATTCCTCTTATTGTTACATTTTACATAGTGGCTCTACTGTTTTTACTTGGTGTGGAAGTCTTACCACCTCAGATGACCAGGAACTTCTTGAAAGATTTCTGGATGTGATAAAGGTGATAGTTATATGTCCTTATGAACACAACATTATAGATGGTTGAGTTCCTATGATAAGAATCATTGAATTACTTGTTACTTGTCAGTGATGTGCCCATTATGAATCAAGCTGCCATTAGAATATGTAAATCATATGTTGTTAATTAAGGGATTTTGTGGAGATGGTCTCCCTATGTAAAATGAAAATGCTTCTGGATAAAAAGTTGATGATCAATTCAATTGAACATAGAATTTTTGTCAACCATTCATTACTTTGAAAGTTTGTTATCTTTTCCACTCTTGTTTCATAGTACATCTGAAGGCCATCTCTGGAAATTTCTTCTCATATGATTCCCAATTTTCTGCAGCCAAACTGTCAATCCAGGCCGCACAAGGAAGGTGCAGAATCTGAACACTTTTGGGGTTTGTTAGGAGGAAAAGTGGAATACCCCAGCCAGAAAATTTCACGGAATAATGAGAGCGATCCACACCTGTTTTCTTGCACTTTTGCTAAAGGTGTGGATAATTGTATTTCGATTTTAAGATGTACTAGTCTTTTGTTAGTATCATCTTTCATTTACAAGTCTAACTTTGTTCTTGGCATTGATTTCGCTGCGGAAATGGTGGCGTCTTGCAGAAGATTTGAAGGTATTCCCTTCTTATTTAGTTCTTGCAATCTTCTCTGAGAAACATGTATCATTAAAATCTGTTTTGAAACTTAAACTGGTGTTCTTATTTCCCTATTTGAAAAAGAAGCAATGCATCAAAGTTCCTGTGTTCTGTGCTGTAAACAAGTCTTATTACATATAGTCAATGGTCAATGCCTTTTTCCAAAATCTTCTCTAAAATTTATGTTATCGCAGGTTGCTGAGATATACAACTTTGGCCAAGATGATCTGATGACAGAAGATATCGACATTCTTAGTTGTCACTCCGAGATCTTTGTTTGGGTTGGTCAACAGGTTGACCCCAAGACTAAAGTACATGCTTTAAATATTGGCGAGGTACGTTTCTAGTTGTAAATTATGAGAACTATTTTTCGCACTCGACATCTTTTTTCGACCAATTGTCAGGCTATTGAATCCTCATTAAAATTTTCGAGTCAATCATTAACTCTTATGTCTGCTACAATGTCTTCTACTGGTGGTTTTCTGGTCACATCTTATTTTGGTTTTTTTCCAAACTGTCGACAGAAATTTCTTGAGATTGATTTTCTTCTCGAAAAGTTATCACGTGAAGCTCCAATATATATTGTCATGGAAGGAAACGAGCCTCCCTTTTTCACCCGTTTCTTTTCGTGGGACTCTGCAAAATCTGCAGTAAGGATTTTATGCTAATTAAGTACTGATTAATTACTAAACTCTGCGAATATTCTGATTGTTTATTTTATTTGGTTTTCCTTTCTCTGTTTCTTTTAATAATTTGAATACAGATGCACGGAAATTCATTCCAGAGAAAGCTTGCATTAGTCAGAAATGGCGGTACTCCTACTGTAGATGTGAGTCATTTTTGGTTGTCCCATTTTTTTTTTACTATTACTTTTCAGATCTAACTACTGCTCTCAACAAGATTTTTAGAATAAGTGAAATGAATAGAACAACCTTTATCGAACTGGCTTAAGCTTTTGGGATTAGTTATAAATGACACGACACAGTATTAGAATAAGAGGTCCTTAAATGAACTCTCTACCTAGTTTCTCATCTTAAAAGGATATTTGAGAAGGCATATCAACTACTATTAGTTTGAAATTGTTACTATGTGNCATATACAAAGTTTTTTTTTTAATAGACATCATGCACTTTATTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCCTTCCTTTTCTAATGGCCACATAACTTGTAATCAGAAACCGAAGCGGAGAACTCCGGTAATGTATGGAGGGAGGTCCAGTAGTGTGCCGGAAAAATCACAGCGCTCGAGGAGTGTGTCGTTCAGTCCTGATCGAGTTCGCGTGAGAGGAAGATCGCCAGCGTTCAATGCATTAGCTGCAAACTTTGAGAACCCGAATGCTAGAAATCTATCTACCCCTCCACCGATGGTTAGAAGGTTATACCCGAAATCTGTTACTCCTGATTCATCAAGGCTGGCTTCTAAAAATGCTGCCATAGCAGCTCTTAGTGCGAGTTTTGAACAGCCACCACCCGCCCGAGAAGCTATTATACCTCGCTCTTTAAGGGGTAATCTAAGTTTCACCACGATTTGTCTACAACCATTATGATCTTCTAATTTAATCGAATCGATTTTCTTGTGTTTCTCAAGGGAGCCTAGATGCCTCCAAGCCAAAACCGGAGTCGGACAACAAGGAGAACTCGATGAGCAACAGATTAGAATCTCTCACCATAGAAGAAGATGTGAAAGAGGGTGAAGCTGAAGATGAGGAAGGCCTAACAATACATCCATACGAATCCCTTACAACATTATCAAGCAATCCAGCTCCAGACATCGACATAACAAAGCGAGAGGTAATTGATCAATCAATCACCAGTTAAACCATTTGATGAAAACTAGCAACACCTTTAACCAATGTCGTGTTGAGTTCTGACGTGCGGAATTTGAAACGTTTACAGGCATACCTGTCATCGTCGGAGTTCAGGGAGAAGTTCGGAATGGCAAAAGATGCTTTCTATAAGCTACCAAAATGGAAGCAGAACAAACTCAAAATGGCCCTGCATTTGTTC

mRNA sequence

ATGGCTGTCTCTATGAGAGATTTGGATCAAGCTTTTCAGGGTGCTGGCCAGAAAGCTGGCCTTGAAGTATGGCGCATCGAAAATTTCCGCCCTGTTCTTGTACCAAAATCATCTCACGGCAAATTTTTCACAGGCGATTCCTATGTAGTCCTGAAGACCTCATCCTTAAAAAGTGGTGCTTTACGGCATGATATTCATTATTGGCTTGGTAAGGATACCACTCAGGATGAAGCCGGTGTTGCAGCCATCAAAACCGTAGAATTGGATGCAGCTCTCGGAGGACGTGCTGTTCAGTACCGGGAAGTGCAAGGTCACGAGACAGAAAAGTTCTTATCATATTTCAAACCGTGCATAATACCGCAAGAAGGTGGTGCTTCATCTGGGTTTAAACATGTAGAGGCTGATGAACACCAGATTCGGTTGTATGTTTGCAAAGGAAAACGTGTTGTTCATGTTAAAGAGGCAAGTCTAACATCAGATATGCAAGCTGATTGGGCATTAGCTTCACCTTTTCTTTTTTGTTTTTTTGGTTTGTTAAGTTGTTTACTCTGGTGGCAGGTTGCTTTTGCTCGATCTTCACTCAACCATGATGATATTTTCATTCTTGATACCAAGTCTAAAATATTCCAATTTAATGGTTCCAACTCATCCATTCAAGAGAGGGCCAAAGCATTGGAAGTAGTTCAATACATCAAAGATACCTATCATGATGGAAAATGTGAGATAGCCACCATTGAGGATGGCAAATTGATGGCAGATGTTGAAACTGGGGAATTTTGGGGTTTATTTGGTGGTTTCGCTCCACTTCCAAGGAAAGTTGCCGGTGATGGTGATAAGACGGTTGATTCACATCCAACTAAACTACTGTGTGTTGTGAAGGGTAATACAGAACCTGTTGAAGCTGATTCTTTAACTAGAGAGCTGTTAGAGACAAATAAATGTTACATTATTGATTGCGGGACAGAAGTTTTTATATGGATGGGAAGAACTACCTCTCTTGATGAAAGGAAAAGTGCAAGCAGAGCTGCAGAAGAGTTAGTTAGTGGTCCTGATCGTCCACAATCGCATATAATGCGTGTGATTGAAGCCTTTGAACCAATAATATTCCGAGCAAAGTTTGATTCATGGCCTGAAACAGCTGCTGTAGCTGTGTCAGAGGATGGTAGAGGCAAGGTTGCAGCACTTTTAAAACGCCAAGGAGTTAATGTGAAGGGTCTTTTGAAAGCTGAACCTGTAAAAGAGGAACCTCAACCTTACATTGATTGTACAGGAAATCTACAGGTTTGGCGTGTCAGTGGTCAGGAAAAGCTTTTACTTCCCGTCTCTGATCAGTCAAAATTTTACACTGGAGATTGCTATATCTTCCAATATTCATACCCGGGAGAAGACAGGGAGGAGTTCCTTATCGGAACTTGGTTTGGGAAACAGAGTGTTGAGGAAGAAAGAACATCTGCTTTATCAATGGCGAGGAAGATGGTGGAATCACTGAAGTTCCTGCCAGTCCAGGCTCGTATTTATGAAGGATATGAACCAATCCAGTTCTATTCAATCTTTCAAAGCTTTATCGTTTATAAGGGTGGGTTGAGTGATGGATACAAAAACTACATTACAGAGAATGAAATTCCAGATGTGACAAATGTAGAAGACGGCGTCGCATTGTTTCGAATCCAGGGCTCTGGCCCTGATAATATGCAAGCAATTCAAGTCAAAGCAGTCGGATCCTCTTTGAATTCCTCTTATTGTTACATTTTACATAGTGGCTCTACTGTTTTTACTTGGTGTGGAAGTCTTACCACCTCAGATGACCAGGAACTTCTTGAAAGATTTCTGGATGTGATAAAGCCAAACTGTCAATCCAGGCCGCACAAGGAAGGTGCAGAATCTGAACACTTTTGGGGTTTGTTAGGAGGAAAAGTGGAATACCCCAGCCAGAAAATTTCACGGAATAATGAGAGCGATCCACACCTGTTTTCTTGCACTTTTGCTAAAGGTGTTGCTGAGATATACAACTTTGGCCAAGATGATCTGATGACAGAAGATATCGACATTCTTAGTTGTCACTCCGAGATCTTTGTTTGGGTTGGTCAACAGGTTGACCCCAAGACTAAAGTACATGCTTTAAATATTGGCGAGAAATTTCTTGAGATTGATTTTCTTCTCGAAAAGTTATCACGTGAAGCTCCAATATATATTGTCATGGAAGGAAACGAGCCTCCCTTTTTCACCCGTTTCTTTTCGTGGGACTCTGCAAAATCTGCAATGCACGGAAATTCATTCCAGAGAAAGCTTGCATTAGTCAGAAATGGCGGTACTCCTACTGTAGATAAACCGAAGCGGAGAACTCCGGTAATGTATGGAGGGAGGTCCAGTAGTGTGCCGGAAAAATCACAGCGCTCGAGGAGTGTGTCGTTCAGTCCTGATCGAGTTCGCGTGAGAGGAAGATCGCCAGCGTTCAATGCATTAGCTGCAAACTTTGAGAACCCGAATGCTAGAAATCTATCTACCCCTCCACCGATGGTTAGAAGGTTATACCCGAAATCTGTTACTCCTGATTCATCAAGGCTGGCTTCTAAAAATGCTGCCATAGCAGCTCTTAGTGCGAGTTTTGAACAGCCACCACCCGCCCGAGAAGCTATTATACCTCGCTCTTTAAGGGGTAATCTAAGGAGCCTAGATGCCTCCAAGCCAAAACCGGAGTCGGACAACAAGGAGAACTCGATGAGCAACAGATTAGAATCTCTCACCATAGAAGAAGATGTGAAAGAGGGTGAAGCTGAAGATGAGGAAGGCCTAACAATACATCCATACGAATCCCTTACAACATTATCAAGCAATCCAGCTCCAGACATCGACATAACAAAGCGAGAGGCATACCTGTCATCGTCGGAGTTCAGGGAGAAGTTCGGAATGGCAAAAGATGCTTTCTATAAGCTACCAAAATGGAAGCAGAACAAACTCAAAATGGCCCTGCATTTGTTC

Coding sequence (CDS)

ATGGCTGTCTCTATGAGAGATTTGGATCAAGCTTTTCAGGGTGCTGGCCAGAAAGCTGGCCTTGAAGTATGGCGCATCGAAAATTTCCGCCCTGTTCTTGTACCAAAATCATCTCACGGCAAATTTTTCACAGGCGATTCCTATGTAGTCCTGAAGACCTCATCCTTAAAAAGTGGTGCTTTACGGCATGATATTCATTATTGGCTTGGTAAGGATACCACTCAGGATGAAGCCGGTGTTGCAGCCATCAAAACCGTAGAATTGGATGCAGCTCTCGGAGGACGTGCTGTTCAGTACCGGGAAGTGCAAGGTCACGAGACAGAAAAGTTCTTATCATATTTCAAACCGTGCATAATACCGCAAGAAGGTGGTGCTTCATCTGGGTTTAAACATGTAGAGGCTGATGAACACCAGATTCGGTTGTATGTTTGCAAAGGAAAACGTGTTGTTCATGTTAAAGAGGCAAGTCTAACATCAGATATGCAAGCTGATTGGGCATTAGCTTCACCTTTTCTTTTTTGTTTTTTTGGTTTGTTAAGTTGTTTACTCTGGTGGCAGGTTGCTTTTGCTCGATCTTCACTCAACCATGATGATATTTTCATTCTTGATACCAAGTCTAAAATATTCCAATTTAATGGTTCCAACTCATCCATTCAAGAGAGGGCCAAAGCATTGGAAGTAGTTCAATACATCAAAGATACCTATCATGATGGAAAATGTGAGATAGCCACCATTGAGGATGGCAAATTGATGGCAGATGTTGAAACTGGGGAATTTTGGGGTTTATTTGGTGGTTTCGCTCCACTTCCAAGGAAAGTTGCCGGTGATGGTGATAAGACGGTTGATTCACATCCAACTAAACTACTGTGTGTTGTGAAGGGTAATACAGAACCTGTTGAAGCTGATTCTTTAACTAGAGAGCTGTTAGAGACAAATAAATGTTACATTATTGATTGCGGGACAGAAGTTTTTATATGGATGGGAAGAACTACCTCTCTTGATGAAAGGAAAAGTGCAAGCAGAGCTGCAGAAGAGTTAGTTAGTGGTCCTGATCGTCCACAATCGCATATAATGCGTGTGATTGAAGCCTTTGAACCAATAATATTCCGAGCAAAGTTTGATTCATGGCCTGAAACAGCTGCTGTAGCTGTGTCAGAGGATGGTAGAGGCAAGGTTGCAGCACTTTTAAAACGCCAAGGAGTTAATGTGAAGGGTCTTTTGAAAGCTGAACCTGTAAAAGAGGAACCTCAACCTTACATTGATTGTACAGGAAATCTACAGGTTTGGCGTGTCAGTGGTCAGGAAAAGCTTTTACTTCCCGTCTCTGATCAGTCAAAATTTTACACTGGAGATTGCTATATCTTCCAATATTCATACCCGGGAGAAGACAGGGAGGAGTTCCTTATCGGAACTTGGTTTGGGAAACAGAGTGTTGAGGAAGAAAGAACATCTGCTTTATCAATGGCGAGGAAGATGGTGGAATCACTGAAGTTCCTGCCAGTCCAGGCTCGTATTTATGAAGGATATGAACCAATCCAGTTCTATTCAATCTTTCAAAGCTTTATCGTTTATAAGGGTGGGTTGAGTGATGGATACAAAAACTACATTACAGAGAATGAAATTCCAGATGTGACAAATGTAGAAGACGGCGTCGCATTGTTTCGAATCCAGGGCTCTGGCCCTGATAATATGCAAGCAATTCAAGTCAAAGCAGTCGGATCCTCTTTGAATTCCTCTTATTGTTACATTTTACATAGTGGCTCTACTGTTTTTACTTGGTGTGGAAGTCTTACCACCTCAGATGACCAGGAACTTCTTGAAAGATTTCTGGATGTGATAAAGCCAAACTGTCAATCCAGGCCGCACAAGGAAGGTGCAGAATCTGAACACTTTTGGGGTTTGTTAGGAGGAAAAGTGGAATACCCCAGCCAGAAAATTTCACGGAATAATGAGAGCGATCCACACCTGTTTTCTTGCACTTTTGCTAAAGGTGTTGCTGAGATATACAACTTTGGCCAAGATGATCTGATGACAGAAGATATCGACATTCTTAGTTGTCACTCCGAGATCTTTGTTTGGGTTGGTCAACAGGTTGACCCCAAGACTAAAGTACATGCTTTAAATATTGGCGAGAAATTTCTTGAGATTGATTTTCTTCTCGAAAAGTTATCACGTGAAGCTCCAATATATATTGTCATGGAAGGAAACGAGCCTCCCTTTTTCACCCGTTTCTTTTCGTGGGACTCTGCAAAATCTGCAATGCACGGAAATTCATTCCAGAGAAAGCTTGCATTAGTCAGAAATGGCGGTACTCCTACTGTAGATAAACCGAAGCGGAGAACTCCGGTAATGTATGGAGGGAGGTCCAGTAGTGTGCCGGAAAAATCACAGCGCTCGAGGAGTGTGTCGTTCAGTCCTGATCGAGTTCGCGTGAGAGGAAGATCGCCAGCGTTCAATGCATTAGCTGCAAACTTTGAGAACCCGAATGCTAGAAATCTATCTACCCCTCCACCGATGGTTAGAAGGTTATACCCGAAATCTGTTACTCCTGATTCATCAAGGCTGGCTTCTAAAAATGCTGCCATAGCAGCTCTTAGTGCGAGTTTTGAACAGCCACCACCCGCCCGAGAAGCTATTATACCTCGCTCTTTAAGGGGTAATCTAAGGAGCCTAGATGCCTCCAAGCCAAAACCGGAGTCGGACAACAAGGAGAACTCGATGAGCAACAGATTAGAATCTCTCACCATAGAAGAAGATGTGAAAGAGGGTGAAGCTGAAGATGAGGAAGGCCTAACAATACATCCATACGAATCCCTTACAACATTATCAAGCAATCCAGCTCCAGACATCGACATAACAAAGCGAGAGGCATACCTGTCATCGTCGGAGTTCAGGGAGAAGTTCGGAATGGCAAAAGATGCTTTCTATAAGCTACCAAAATGGAAGCAGAACAAACTCAAAATGGCCCTGCATTTGTTC

Protein sequence

MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGALRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLSCLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCEIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVEADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRVIEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYIDCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEEERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENEIPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTSDDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCTFAKGVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEIDFLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKPKRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPPPMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDASKPKPESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF
Homology
BLAST of MS002230 vs. NCBI nr
Match: XP_022135030.1 (villin-4-like isoform X1 [Momordica charantia] >XP_022135031.1 villin-4-like isoform X1 [Momordica charantia])

HSP 1 Score: 1889.8 bits (4894), Expect = 0.0e+00
Identity = 952/996 (95.58%), Postives = 954/996 (95.78%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA
Sbjct: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKE                          
Sbjct: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKE-------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 VAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC
Sbjct: 181 ------VAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVA DGDKTVDSHPTKLLCVVKGNTEPVE
Sbjct: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVASDGDKTVDSHPTKLLCVVKGNTEPVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
           ADSLTRELLETNKCYIIDCGTEVFIWMGR TSLDERKSASRAAEELVSGPDRPQSHIMRV
Sbjct: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRATSLDERKSASRAAEELVSGPDRPQSHIMRV 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420
           IE FEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI
Sbjct: 361 IEGFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420

Query: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480
           DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE
Sbjct: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480

Query: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540
           ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE
Sbjct: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540

Query: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600
           IPDVTNVEDGVALFRIQGSGPDNMQAIQV+AVGSSLNSSYCYILHSGSTVFTWCGSLTTS
Sbjct: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVEAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600

Query: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660
           DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT
Sbjct: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660

Query: 661 FAK---GVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 720
           FAK    VAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID
Sbjct: 661 FAKEDLKVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 720

Query: 721 FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780
           FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP
Sbjct: 721 FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780

Query: 781 KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840
           KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP
Sbjct: 781 KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840

Query: 841 PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDASKPK 900
           PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG   SLDASKPK
Sbjct: 841 PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG---SLDASKPK 900

Query: 901 PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA 960
           PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA
Sbjct: 901 PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA 960

Query: 961 YLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           YLSSSEFREKFGMAKDAF+KLPKWKQNKLKMALHLF
Sbjct: 961 YLSSSEFREKFGMAKDAFHKLPKWKQNKLKMALHLF 961

BLAST of MS002230 vs. NCBI nr
Match: XP_022135032.1 (villin-4-like isoform X2 [Momordica charantia])

HSP 1 Score: 1830.8 bits (4741), Expect = 0.0e+00
Identity = 929/996 (93.27%), Postives = 931/996 (93.47%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA
Sbjct: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKE                          
Sbjct: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKE-------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 VAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC
Sbjct: 181 ------VAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVA DGDKTVDSHPTKLLCVVKGNTEPVE
Sbjct: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVASDGDKTVDSHPTKLLCVVKGNTEPVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
           ADSLTRELLETNKCYIIDCGTEVFIWMGR TSLDERKSASRAAEELVSGPDRPQSHIMRV
Sbjct: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRATSLDERKSASRAAEELVSGPDRPQSHIMRV 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420
           IE FEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI
Sbjct: 361 IEGFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420

Query: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480
           DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE
Sbjct: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480

Query: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540
           ERTSALSMARKMVESLKFLPVQ                       GGLSDGYKNYITENE
Sbjct: 481 ERTSALSMARKMVESLKFLPVQ-----------------------GGLSDGYKNYITENE 540

Query: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600
           IPDVTNVEDGVALFRIQGSGPDNMQAIQV+AVGSSLNSSYCYILHSGSTVFTWCGSLTTS
Sbjct: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVEAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600

Query: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660
           DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT
Sbjct: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660

Query: 661 FAK---GVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 720
           FAK    VAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID
Sbjct: 661 FAKEDLKVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 720

Query: 721 FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780
           FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP
Sbjct: 721 FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780

Query: 781 KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840
           KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP
Sbjct: 781 KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840

Query: 841 PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDASKPK 900
           PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG   SLDASKPK
Sbjct: 841 PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG---SLDASKPK 900

Query: 901 PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA 960
           PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA
Sbjct: 901 PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA 938

Query: 961 YLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           YLSSSEFREKFGMAKDAF+KLPKWKQNKLKMALHLF
Sbjct: 961 YLSSSEFREKFGMAKDAFHKLPKWKQNKLKMALHLF 938

BLAST of MS002230 vs. NCBI nr
Match: XP_008453585.2 (PREDICTED: LOW QUALITY PROTEIN: villin-4 [Cucumis melo])

HSP 1 Score: 1781.9 bits (4614), Expect = 0.0e+00
Identity = 888/996 (89.16%), Postives = 921/996 (92.47%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFF GDSY+VLKT+SLKSGA
Sbjct: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFMGDSYIVLKTTSLKSGA 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           LRHDIHYWLGKDTTQDEAG AAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LRHDIHYWLGKDTTQDEAGTAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGG SSGFKH EA+EH+  LYVCKGKRVVHVKE                          
Sbjct: 121 QEGGVSSGFKHAEAEEHKTTLYVCKGKRVVHVKE-------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 V FARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC
Sbjct: 181 ------VPFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           E+A IEDGKLMADVETGEFWGLFGGFAPLPRKVAG+GDKTVDSHPTKLLCV KGN EPVE
Sbjct: 241 EVAAIEDGKLMADVETGEFWGLFGGFAPLPRKVAGEGDKTVDSHPTKLLCVEKGNREPVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
           ADSL RELLETNKCYI+D GTEVFIWMGR +SL+ERK+ASRAAEELVS PDRPQSHIMRV
Sbjct: 301 ADSLARELLETNKCYILDSGTEVFIWMGRNSSLEERKNASRAAEELVSVPDRPQSHIMRV 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420
           IE FEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQG+NVKGLLKAEPVKEEPQPYI
Sbjct: 361 IEGFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGINVKGLLKAEPVKEEPQPYI 420

Query: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480
           DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSY GED+EEFL+GTWFGKQSVE 
Sbjct: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYSGEDKEEFLVGTWFGKQSVEG 480

Query: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540
           ER +ALS+A KMVESLKFLPVQAR+YEG+EPIQFYSIFQSFIV+KGGLSDGYKNYITENE
Sbjct: 481 ERAAALSLASKMVESLKFLPVQARLYEGHEPIQFYSIFQSFIVFKGGLSDGYKNYITENE 540

Query: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600
           IPDVTN EDGVALFR+QGSGP+NMQAIQV AVGSSLNSSYCYILHSGSTVFTWCGSLT +
Sbjct: 541 IPDVTNSEDGVALFRVQGSGPENMQAIQVDAVGSSLNSSYCYILHSGSTVFTWCGSLTNT 600

Query: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660
           DDQEL+ERFLDVIKPNCQS+PHKEGAESE FW LLGGKVEYPSQKI+RNNESDPHLFSCT
Sbjct: 601 DDQELVERFLDVIKPNCQSKPHKEGAESEQFWDLLGGKVEYPSQKIARNNESDPHLFSCT 660

Query: 661 FAK---GVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 720
           FA     VAEIYNFGQDDLMTEDIDILSCHS+IFVWVGQQVDPKTKVHAL IGEKFLEID
Sbjct: 661 FANEILKVAEIYNFGQDDLMTEDIDILSCHSDIFVWVGQQVDPKTKVHALKIGEKFLEID 720

Query: 721 FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780
           F LEKLSRE PIYIVMEG+EPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP
Sbjct: 721 FXLEKLSRETPIYIVMEGSEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780

Query: 781 KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840
           KRR PV+YGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP
Sbjct: 781 KRRAPVIYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840

Query: 841 PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDASKPK 900
           PMVR+LYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG+L SL   KPK
Sbjct: 841 PMVRKLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGSLGSL---KPK 900

Query: 901 PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA 960
           PESDNKENSMSNR+ESLTI EDVKEGE EDEEGLTIHPYESLTT SSNP  +ID+TKRE 
Sbjct: 901 PESDNKENSMSNRIESLTIAEDVKEGEVEDEEGLTIHPYESLTTNSSNPVSEIDVTKRET 960

Query: 961 YLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           YLSS+EFREKFGMAKDAFYKLPKWKQNKLKMALHLF
Sbjct: 961 YLSSAEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 961

BLAST of MS002230 vs. NCBI nr
Match: XP_038879573.1 (villin-4-like isoform X1 [Benincasa hispida] >XP_038879575.1 villin-4-like isoform X1 [Benincasa hispida] >XP_038879576.1 villin-4-like isoform X1 [Benincasa hispida] >XP_038879577.1 villin-4-like isoform X1 [Benincasa hispida] >XP_038879578.1 villin-4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1772.7 bits (4590), Expect = 0.0e+00
Identity = 885/996 (88.86%), Postives = 916/996 (91.97%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFF GDSY+VLKTSSLKSGA
Sbjct: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFMGDSYIVLKTSSLKSGA 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           LRHDIHYWLGKDTTQDEAG AAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LRHDIHYWLGKDTTQDEAGTAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGG SSGFKH EA+EH+  LYVCKGKRVVHVKE                          
Sbjct: 121 QEGGVSSGFKHAEAEEHKTTLYVCKGKRVVHVKE-------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 V FARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC
Sbjct: 181 ------VPFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           E+A IEDGKLMADVETGEFWGLFGGFAPLPRKV  +GDKTVDSHPTKLLCV KGN EPVE
Sbjct: 241 EVAAIEDGKLMADVETGEFWGLFGGFAPLPRKVVSEGDKTVDSHPTKLLCVEKGNREPVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
           ADS+TRELLETNKCYI+DCGTEVFIWMGR TSLD RK+ASRAAEELVS PDR QSHIMRV
Sbjct: 301 ADSITRELLETNKCYILDCGTEVFIWMGRHTSLDGRKNASRAAEELVSDPDRLQSHIMRV 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420
           IE FEPIIFRAKFDSWPETAAV+VSEDGRGKVAALLKRQG+NVKGLLKA+PVKEEPQPYI
Sbjct: 361 IEGFEPIIFRAKFDSWPETAAVSVSEDGRGKVAALLKRQGINVKGLLKADPVKEEPQPYI 420

Query: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480
           DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSY GED+EE+L+GTWFGKQSVE 
Sbjct: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYSGEDKEEYLVGTWFGKQSVEG 480

Query: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540
           ER SALS+A KMVESLKFLPVQARIYEGYEPIQFYSIFQSFIV+KGGLSDGYKNYITENE
Sbjct: 481 ERASALSLASKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVFKGGLSDGYKNYITENE 540

Query: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600
           IPDVTN EDGVALFR+QGSGP+NMQAIQV+AVGSSLNSSY YILHSGSTVFTWCGSLT +
Sbjct: 541 IPDVTNSEDGVALFRVQGSGPENMQAIQVEAVGSSLNSSYSYILHSGSTVFTWCGSLTNA 600

Query: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660
           DDQEL+ERFLDVIKPNCQS+ HKEGAESE FW LLGGK+EYPSQKI+RN+ESDPHLFSCT
Sbjct: 601 DDQELVERFLDVIKPNCQSKQHKEGAESEQFWDLLGGKLEYPSQKIARNSESDPHLFSCT 660

Query: 661 FAK---GVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 720
           FAK    VAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQ+DPKTKVHAL IGEKFLEID
Sbjct: 661 FAKENLKVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQLDPKTKVHALKIGEKFLEID 720

Query: 721 FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780
           F LEKL RE PIYIVMEG+EPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP
Sbjct: 721 FFLEKLPRETPIYIVMEGSEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780

Query: 781 KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840
           KRR PVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP
Sbjct: 781 KRRAPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840

Query: 841 PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDASKPK 900
           PMVRRLYPKSV PDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG   SL A KPK
Sbjct: 841 PMVRRLYPKSVAPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG---SLGALKPK 900

Query: 901 PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA 960
           PESDNKENSMSNR+ESLTI+EDVKEGE EDEEGLTIHPYESLTT SSNP  DID+TKRE 
Sbjct: 901 PESDNKENSMSNRIESLTIQEDVKEGEIEDEEGLTIHPYESLTTNSSNPVSDIDVTKRET 960

Query: 961 YLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           YLSS+EFREKFGM KDAFYKLPKWKQNKLKMALHLF
Sbjct: 961 YLSSAEFREKFGMTKDAFYKLPKWKQNKLKMALHLF 961

BLAST of MS002230 vs. NCBI nr
Match: TYK11812.1 (villin-4 [Cucumis melo var. makuwa])

HSP 1 Score: 1769.2 bits (4581), Expect = 0.0e+00
Identity = 883/998 (88.48%), Postives = 918/998 (91.98%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFF GDSY+VLKT+SLKSGA
Sbjct: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFMGDSYIVLKTTSLKSGA 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           LRHDIHYWLGKDTTQDEAG AAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LRHDIHYWLGKDTTQDEAGTAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGG SSGFKH EA+EH+  LYVCKGKRVVH                             
Sbjct: 121 QEGGVSSGFKHAEAEEHKTTLYVCKGKRVVH----------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 V FARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC
Sbjct: 181 ------VPFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           E+A IEDGKLMADVETGEFWGLFGGFAPLPRKVAG+GDKTVDSHPTKLLCV KGN EPVE
Sbjct: 241 EVAAIEDGKLMADVETGEFWGLFGGFAPLPRKVAGEGDKTVDSHPTKLLCVEKGNREPVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
           ADSL RELLETNKCYI+D GTEVFIWMGR +SL+ERK+ASRAAEELVS PDRPQSHIMRV
Sbjct: 301 ADSLARELLETNKCYILDSGTEVFIWMGRNSSLEERKNASRAAEELVSVPDRPQSHIMRV 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420
           IE FEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQG+NVKGLLKAEPVKEEPQPYI
Sbjct: 361 IEGFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGINVKGLLKAEPVKEEPQPYI 420

Query: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480
           DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSY GED+EEFL+GTWFGKQSVE 
Sbjct: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYSGEDKEEFLVGTWFGKQSVEG 480

Query: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540
           ER +ALS+A KMVESLKFLPVQAR+YEG+EPIQFYSIFQSFIV+KGGLSDGYKNYITENE
Sbjct: 481 ERAAALSLASKMVESLKFLPVQARLYEGHEPIQFYSIFQSFIVFKGGLSDGYKNYITENE 540

Query: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600
           IPDVTN EDGVALFR+QGSGP+NMQAIQV AVGSSLNSSYCYILHSGSTVFTWCGSLT +
Sbjct: 541 IPDVTNSEDGVALFRVQGSGPENMQAIQVDAVGSSLNSSYCYILHSGSTVFTWCGSLTNT 600

Query: 601 DDQELLERFLDVIK-PNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSC 660
           DDQEL+ERFLDVIK PNCQS+PHKEGAESE FW LLGGKVEYPSQKI+RNNESDPHLFSC
Sbjct: 601 DDQELVERFLDVIKVPNCQSKPHKEGAESEQFWDLLGGKVEYPSQKIARNNESDPHLFSC 660

Query: 661 TFAKG----VAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLE 720
           TFA G    ++ IYNFGQDDLMTEDIDILSCHS+IFVWVGQQVDPKTKVHAL IGEKFLE
Sbjct: 661 TFANGAYNSISIIYNFGQDDLMTEDIDILSCHSDIFVWVGQQVDPKTKVHALKIGEKFLE 720

Query: 721 IDFLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVD 780
           IDF LEKLSRE PIYIVMEG+EPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVD
Sbjct: 721 IDFFLEKLSRETPIYIVMEGSEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVD 780

Query: 781 KPKRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLST 840
           KPKRR PV+YGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLST
Sbjct: 781 KPKRRAPVIYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLST 840

Query: 841 PPPMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDASK 900
           PPPMVR+LYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG+L SL   K
Sbjct: 841 PPPMVRKLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGSLGSL---K 900

Query: 901 PKPESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKR 960
           PKPESDNKENSMSNR+ESLTI EDVKEGE EDEEGLTIHPYESLTT SSNP  +ID+TKR
Sbjct: 901 PKPESDNKENSMSNRIESLTIAEDVKEGEVEDEEGLTIHPYESLTTNSSNPVSEIDVTKR 960

Query: 961 EAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           E YLSS+EFREKFGMAKDAFYKLPKWKQNKLKMALHLF
Sbjct: 961 ETYLSSAEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 960

BLAST of MS002230 vs. ExPASy Swiss-Prot
Match: O65570 (Villin-4 OS=Arabidopsis thaliana OX=3702 GN=VLN4 PE=1 SV=1)

HSP 1 Score: 1414.4 bits (3660), Expect = 0.0e+00
Identity = 708/1009 (70.17%), Postives = 821/1009 (81.37%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           M+VSMRDLD AFQGAGQKAG+E+WRIENF P  +PKSS GKFFTGDSY+VLKT++LK+GA
Sbjct: 1   MSVSMRDLDPAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGA 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           LRHDIHYWLGKDT+QDEAG AA+KTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LRHDIHYWLGKDTSQDEAGTAAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGG +SGFKHV A+EH  RL+VC+GK VVHVKE                          
Sbjct: 121 QEGGVASGFKHVVAEEHITRLFVCRGKHVVHVKE-------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 V FARSSLNHDDI+ILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDG C
Sbjct: 181 ------VPFARSSLNHDDIYILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGTC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           E+AT+EDGKLMAD ++GEFWG FGGFAPLPRK A D DKT +S  T+L CV KG   PVE
Sbjct: 241 EVATVEDGKLMADADSGEFWGFFGGFAPLPRKTANDEDKTYNSDITRLFCVEKGQANPVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
            D+L RE+L+TNKCYI+DCG EVF+WMGRTTSLD+RK AS+AAEE++   +RP+S ++R+
Sbjct: 301 GDTLKREMLDTNKCYILDCGIEVFVWMGRTTSLDDRKIASKAAEEMIRSSERPKSQMIRI 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420
           IE FE + FR+KF+SW +     VSEDGRG+VAALL+RQGVNV+GL+KA P KEEPQ +I
Sbjct: 361 IEGFETVPFRSKFESWTQETNTTVSEDGRGRVAALLQRQGVNVRGLMKAAPPKEEPQVFI 420

Query: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480
           DCTGNLQVWRV+GQ K LL  +D SKFY+GDCY+FQYSYPGE++EE LIGTWFGKQSVEE
Sbjct: 421 DCTGNLQVWRVNGQAKTLLQAADHSKFYSGDCYVFQYSYPGEEKEEVLIGTWFGKQSVEE 480

Query: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540
           ER SA+SMA KMVES+KF+P QARIYEG EPIQF+ I QSFIV+KGG+S GYK YI E E
Sbjct: 481 ERGSAVSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVFKGGISSGYKKYIAEKE 540

Query: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600
           + D T  E+GVALFRIQGSGP+NMQAIQV  V +SLNSSY YILH+ S+VFTW G+L+T+
Sbjct: 541 VDDDTYNENGVALFRIQGSGPENMQAIQVDPVAASLNSSYYYILHNDSSVFTWAGNLSTA 600

Query: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660
            DQEL ER LD+IKPN QSR  KEG+ESE FW LLGGK EY SQK+++  E DPHLFSCT
Sbjct: 601 TDQELAERQLDLIKPNQQSRAQKEGSESEQFWELLGGKAEYSSQKLTKEPERDPHLFSCT 660

Query: 661 FAK---GVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 720
           F K    V EIYNF QDDLMTEDI I+ CHSEIFVWVGQ+V PK K+ AL IGEKF+E D
Sbjct: 661 FTKEVLKVTEIYNFTQDDLMTEDIFIIDCHSEIFVWVGQEVVPKNKLLALTIGEKFIEKD 720

Query: 721 FLLEKLSREAPIYIVMEGNEPPFFTRFF-SWDSAKSAMHGNSFQRKLALVRNGGTPTVDK 780
            LLEKLS EAPIY++MEG EP FFTRFF SWDS+KSAMHGNSFQRKL +V+NGGTP  DK
Sbjct: 721 SLLEKLSPEAPIYVIMEGGEPSFFTRFFTSWDSSKSAMHGNSFQRKLKIVKNGGTPVADK 780

Query: 781 PKRRTPVMYGGRSSSVPEKS-QRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLST 840
           PKRRTP  YGGR +SVP+KS QRSRS+SFSPDRVRVRGRSPAFNALAA FE+ NARNLST
Sbjct: 781 PKRRTPASYGGR-ASVPDKSQQRSRSMSFSPDRVRVRGRSPAFNALAATFESQNARNLST 840

Query: 841 PPPMVRRLYPKSVTPDSSRL--ASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDA 900
           PPP+VR+LYP+SVTPDSS+   A K++AIA+ SA FE+ PP +E  IP+ ++ + ++ ++
Sbjct: 841 PPPVVRKLYPRSVTPDSSKFAPAPKSSAIASRSALFEKIPP-QEPSIPKPVKASPKTPES 900

Query: 901 SKP---------KPESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSS 960
             P         K E+D +E SMS+R+ESLTI+ED KEG  EDEE L  HPY+ L T S+
Sbjct: 901 PAPESNSKEQEEKKENDKEEGSMSSRIESLTIQEDAKEG-VEDEEDLPAHPYDRLKTTST 960

Query: 961 NPAPDIDITKREAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           +P  DID+T+REAYLSS EF+EKFGM K+AFYKLPKWKQNK KMA+ LF
Sbjct: 961 DPVSDIDVTRREAYLSSEEFKEKFGMTKEAFYKLPKWKQNKFKMAVQLF 974

BLAST of MS002230 vs. ExPASy Swiss-Prot
Match: Q0J716 (Villin-5 OS=Oryza sativa subsp. japonica OX=39947 GN=VLN5 PE=3 SV=1)

HSP 1 Score: 1349.0 bits (3490), Expect = 0.0e+00
Identity = 656/1001 (65.53%), Postives = 808/1001 (80.72%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           M+VSM+DLD AF+GAGQK GLE+WRIENF+PV +P SS+GKFF GDSY++LKT++LK+G+
Sbjct: 1   MSVSMKDLDPAFRGAGQKEGLEIWRIENFKPVPIPASSYGKFFMGDSYIILKTTALKNGS 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           LRHDIHYW+GKDT+QDE+G AAI TVELDAALGGRAVQYRE+QG+ET+KFLSYF+PCI+P
Sbjct: 61  LRHDIHYWIGKDTSQDESGTAAILTVELDAALGGRAVQYREIQGNETDKFLSYFRPCIMP 120

Query: 121 QEGGASSGFKHVEAD--EHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGL 180
           Q GG +SGFKHVE +  EH+ RLYVC G RVVHVKE                        
Sbjct: 121 QPGGVASGFKHVEVNEQEHETRLYVCTGNRVVHVKE------------------------ 180

Query: 181 LSCLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDG 240
                   V FARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDT+H+G
Sbjct: 181 --------VPFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTFHEG 240

Query: 241 KCEIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEP 300
           KCE+A +EDG+LMAD E GEFWG FGGFAPLPR+   + ++  +    KLLC  +G  EP
Sbjct: 241 KCEVAAVEDGRLMADAEAGEFWGFFGGFAPLPRRAPVEDNEKYEETVFKLLCFNQGKLEP 300

Query: 301 VEADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIM 360
           +  +SL  ELL+TNKCY++DCG E+F+WMGRTTSL ERKSAS AAE+L+S  +R ++H++
Sbjct: 301 INYESLLHELLKTNKCYLLDCGVELFVWMGRTTSLQERKSASEAAEKLLSDDNRTKTHVI 360

Query: 361 RVIEAFEPIIFRAKFDSWPETAAVAV-SEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQ 420
           +VIE FE ++F++KF  WP+T  + + SEDGRGKVAALLKRQG+NVKGL+KA P KEEPQ
Sbjct: 361 KVIEGFETVMFKSKFKEWPQTPDLKLSSEDGRGKVAALLKRQGLNVKGLMKAAPAKEEPQ 420

Query: 421 PYIDCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQS 480
            YIDCTG+LQVWR++ ++K+LLP +DQSKFYTGDCYIFQY YPG+D+EE LIG+WFGK+S
Sbjct: 421 AYIDCTGSLQVWRINDKDKILLPSADQSKFYTGDCYIFQYMYPGDDKEECLIGSWFGKKS 480

Query: 481 VEEERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYIT 540
           +EE+R +A+S+A KMVES KF  VQ R+YEG EPIQF+ IFQSF V+KGGLS GYK +I 
Sbjct: 481 IEEDRVTAISLASKMVESAKFQAVQTRLYEGKEPIQFFVIFQSFQVFKGGLSSGYKKFIA 540

Query: 541 ENEIPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSL 600
           EN I D T +EDG+ALFRIQGSGP+NMQAIQV A  SSLNSSY YILH G+TVFTW G+L
Sbjct: 541 ENGIDDDTYLEDGLALFRIQGSGPENMQAIQVDAAASSLNSSYSYILHDGNTVFTWTGNL 600

Query: 601 TTSDDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLF 660
           TTS DQE++ER LD+IKPN QSR  KEG+E++ FW LLGGK EYPSQKI R NESDPHLF
Sbjct: 601 TTSLDQEVVERQLDIIKPNSQSRSQKEGSETDQFWSLLGGKSEYPSQKIGRANESDPHLF 660

Query: 661 SCTFAKG---VAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFL 720
           SC   KG   + EIY+F QDDLMTED+ IL CHS+IFVWVGQQVD K ++ AL+IGEKF+
Sbjct: 661 SCILPKGNLKIKEIYHFTQDDLMTEDVFILDCHSDIFVWVGQQVDVKVRLQALDIGEKFV 720

Query: 721 EIDFLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTV 780
           ++DFL+E LS + PI+++MEG+EP FFTRFF+WDSAKS MHGNS+QRKL++V+ GG+P +
Sbjct: 721 KLDFLMENLSSDTPIFVIMEGSEPTFFTRFFTWDSAKSLMHGNSYQRKLSIVKGGGSPAL 780

Query: 781 DKPKRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLS 840
           DKPKRRTP  Y GR S+V +KSQRSRS+SFSP+RVRVRGRSPAF ALAANFE+ N+RNLS
Sbjct: 781 DKPKRRTPT-YSGR-STVQDKSQRSRSMSFSPERVRVRGRSPAFTALAANFESANSRNLS 840

Query: 841 TPPPMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDAS 900
           TPPP+V++LYPKS TPDSS   SK++A A+L+ SF++P   ++              +  
Sbjct: 841 TPPPVVKKLYPKSATPDSSSAPSKSSATASLTGSFDRPKSVKD------------GSELE 900

Query: 901 KPKPESDNKE--NSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDI 960
           KPK E D KE  N+M++R+ESLTI EDVKE E ED+EGL ++PY+ L T +++P  +ID+
Sbjct: 901 KPKQEEDAKEGINTMTSRVESLTINEDVKENEPEDDEGLPVYPYDRLITTAADPVTEIDV 955

Query: 961 TKREAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           T+RE YLSS+EF++KFGM K+AF KLPKWKQN++K+AL LF
Sbjct: 961 TRRETYLSSAEFKDKFGMTKEAFSKLPKWKQNRMKIALQLF 955

BLAST of MS002230 vs. ExPASy Swiss-Prot
Match: Q67U26 (Villin-3 OS=Oryza sativa subsp. japonica OX=39947 GN=VLN3 PE=2 SV=2)

HSP 1 Score: 1322.0 bits (3420), Expect = 0.0e+00
Identity = 651/1006 (64.71%), Postives = 800/1006 (79.52%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           MAVSMR++D  FQGAGQK GLE+WRIE  + V VPK SHG+FFTGDSYV+LKT++LK+G+
Sbjct: 1   MAVSMREVDAVFQGAGQKDGLEIWRIEKLQAVPVPKESHGRFFTGDSYVILKTTALKNGS 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
            RHDIHYWLGKDT+QDEAG AAIKTVELDAALGGRAVQYREVQG+ETE+FLSYFKPCIIP
Sbjct: 61  FRHDIHYWLGKDTSQDEAGTAAIKTVELDAALGGRAVQYREVQGNETERFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEAD--EHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGL 180
           +EGG +SGF+H E +  EH  RL+VC+GK  VHVKE                        
Sbjct: 121 EEGGIASGFRHTEINEREHVTRLFVCRGKHTVHVKE------------------------ 180

Query: 181 LSCLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDG 240
                   V FARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQY+KD+ H+G
Sbjct: 181 --------VPFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYLKDSNHEG 240

Query: 241 KCEIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEP 300
           KC++ ++EDGKLMAD + GEFWGLFGGFAPLPRK   D +    +  +KL+C+ KG T P
Sbjct: 241 KCDVGSVEDGKLMADADAGEFWGLFGGFAPLPRKTFSDLNGKDSAFSSKLICLNKGQTVP 300

Query: 301 VEADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIM 360
           V+ D LTRELL++ KCY++DCG+E+++WMGR T L+ERK A  AAEEL+   +RP+SHI+
Sbjct: 301 VDFDVLTRELLDSTKCYLLDCGSEIYVWMGRETPLEERKRAGSAAEELLREVNRPKSHIV 360

Query: 361 RVIEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQP 420
           R++E FE +IFR+KF  WP+ A   VS++ RGKVAALLKRQG NVKGL KA PVKEEPQP
Sbjct: 361 RLMEGFETVIFRSKFSKWPKKADAVVSDESRGKVAALLKRQGFNVKGLAKAAPVKEEPQP 420

Query: 421 YIDCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSV 480
            IDCTGNLQVWRV+G EK  L  S+Q KFY+GDCYIFQYSYPGE+ EE LIGTWFGK+SV
Sbjct: 421 QIDCTGNLQVWRVNGTEKTFLSFSEQCKFYSGDCYIFQYSYPGEEGEECLIGTWFGKKSV 480

Query: 481 EEERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITE 540
           ++E+T+A+S+A KMVESLKF  V  R+YEG EP +F+SIFQ+ +++KGG+S GYK +++E
Sbjct: 481 QDEKTTAISVASKMVESLKFQAVMVRLYEGKEPAEFFSIFQNLVIFKGGVSTGYKKFVSE 540

Query: 541 NEIPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLT 600
           N I D T  E+GVALFR+QGSGP+NMQAIQV    +SLNSSYCY+LH G T+FTW G+L+
Sbjct: 541 NGIEDDTYSENGVALFRVQGSGPENMQAIQVDTAATSLNSSYCYVLHDGDTLFTWIGNLS 600

Query: 601 TSDDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFS 660
           +S DQEL ER LDVIKPN QSR  KEG+E + FW LLG K EYPSQKI+++ ESDPHLFS
Sbjct: 601 SSMDQELAERQLDVIKPNLQSRMLKEGSEYDQFWKLLGVKSEYPSQKIAKDQESDPHLFS 660

Query: 661 CTFAKG---VAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLE 720
           CTF+KG   V EI+NF QDDLMTED+ IL CHS +FVWVGQ+VD K +  AL++GEKFLE
Sbjct: 661 CTFSKGVLKVREIFNFTQDDLMTEDVFILDCHSCVFVWVGQRVDTKMRAQALSVGEKFLE 720

Query: 721 IDFLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVD 780
           +D L+E  S+E P+Y++ EG+EP FFTRFF+WDSAKSAMHGNSF+R+L++V++G  P +D
Sbjct: 721 LDILMENSSQETPVYVITEGSEPQFFTRFFTWDSAKSAMHGNSFERRLSIVKDGVKPKLD 780

Query: 781 KPKRR--TPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNL 840
           KPKRR  T   + GR SSVPEKSQRSRS+SFSPDRVRVRGRSPAFNALAANFENPNARNL
Sbjct: 781 KPKRRPTTSSSHTGR-SSVPEKSQRSRSMSFSPDRVRVRGRSPAFNALAANFENPNARNL 840

Query: 841 STPPPMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG----NLR 900
           STPPP +R+  PKS + D ++   + A+IAA+SASFE+P P    +IP+S++     N  
Sbjct: 841 STPPPAIRKPSPKSPSSDPTKPPQRAASIAAISASFERPRP---TLIPKSIKASPDVNKP 900

Query: 901 SLDASKPKPESDNKENSMSNRLESL--TIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPA 960
            ++ASKPKPE++ K+++ S    ++  TI+ED+KEG+ E+EEGL ++PYE L T S NP 
Sbjct: 901 QVEASKPKPEANGKDSTPSKDSPTVTPTIQEDLKEGQPENEEGLPVYPYERLRTSSINPV 960

Query: 961 PDIDITKREAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
            DID+TKRE YLS++EFRE+FGM K+AF KLPKWKQN+LK+AL LF
Sbjct: 961 TDIDVTKRETYLSAAEFRERFGMTKEAFAKLPKWKQNRLKIALQLF 970

BLAST of MS002230 vs. ExPASy Swiss-Prot
Match: Q9LVC6 (Villin-5 OS=Arabidopsis thaliana OX=3702 GN=VLN5 PE=2 SV=1)

HSP 1 Score: 1304.7 bits (3375), Expect = 0.0e+00
Identity = 650/1004 (64.74%), Postives = 786/1004 (78.29%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           M  SMRDLDQA QGAGQK+G+E+WRIENF+PV VP+ SHGKFFTGDSY+VLKT++ +SG+
Sbjct: 1   MTFSMRDLDQALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGS 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           L HDIHYWLGKD++QDEAG  A+ TVELD+ALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LHHDIHYWLGKDSSQDEAGAVAVMTVELDSALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGG +SGF HV+ +EHQ RLY+CKGK VV VKE                          
Sbjct: 121 QEGGVASGFNHVKPEEHQTRLYICKGKHVVRVKE-------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 V F RS+LNH+D+FILDT+SKIFQF+GS SSIQERAKALEVVQYIKDTYHDGKC
Sbjct: 181 ------VPFVRSTLNHEDVFILDTESKIFQFSGSKSSIQERAKALEVVQYIKDTYHDGKC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           +IA +EDG++MAD E GEFWGLFGGFAPLP+K A + D+T  S   KL  V KG T+ VE
Sbjct: 241 DIAAVEDGRMMADAEAGEFWGLFGGFAPLPKKPAVNDDETAASDGIKLFSVEKGQTDAVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
           A+ LT+ELL+TNKCYI+DCG E+F+W GR+TS+D+RKSA+ AAEE     + P+S+++ V
Sbjct: 301 AECLTKELLDTNKCYILDCGLELFVWKGRSTSIDQRKSATEAAEEFFRSSEPPKSNLVSV 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLK--AEPVKEEPQP 420
           +E +E ++FR+KFDSWP ++ +A  + GRGKVAALL+RQGVNV+GL+K  +   K+EP+P
Sbjct: 361 MEGYETVMFRSKFDSWPASSTIAEPQQGRGKVAALLQRQGVNVQGLVKTSSSSSKDEPKP 420

Query: 421 YIDCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSV 480
           YID TGNLQVWR++ +EK+LL  ++QSKFY+GDCYI QYSYPGEDREE L+GTWFGKQSV
Sbjct: 421 YIDGTGNLQVWRINCEEKILLEAAEQSKFYSGDCYILQYSYPGEDREEHLVGTWFGKQSV 480

Query: 481 EEERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITE 540
           EE+R SA+S+A KMVES+KF+P QARI EG EPIQF+ I QSFI +KGG+SD +K YI E
Sbjct: 481 EEDRASAISLANKMVESMKFVPAQARINEGKEPIQFFVIMQSFITFKGGVSDAFKKYIAE 540

Query: 541 NEIPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLT 600
           N+IPD T   +GVALFR+QGSGP+NMQAIQ++A  + LNSS+CYILH  STVFTWCG+LT
Sbjct: 541 NDIPDTTYEAEGVALFRVQGSGPENMQAIQIEAASAGLNSSHCYILHGDSTVFTWCGNLT 600

Query: 601 TSDDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFS 660
           +S+DQEL+ER LD+IKPN  ++  KEG+ESE FW LLGGK EYPSQKI R+ ESDPHLFS
Sbjct: 601 SSEDQELMERMLDLIKPNEPTKAQKEGSESEQFWELLGGKSEYPSQKIKRDGESDPHLFS 660

Query: 661 CTFAK---GVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLE 720
           CT+        EI+NF QDDLMTEDI IL CH+E+FVWVGQQVDPK K  AL+IGE FL+
Sbjct: 661 CTYTNESLKATEIFNFTQDDLMTEDIFILDCHTEVFVWVGQQVDPKKKPQALDIGENFLK 720

Query: 721 IDFLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVD 780
            DFLLE L+ E PIYIV EGNEPPFFTRFF+WDS+KS MHG+SFQRKLA++ N G P +D
Sbjct: 721 HDFLLENLASETPIYIVTEGNEPPFFTRFFTWDSSKSGMHGDSFQRKLAILTNKGKPLLD 780

Query: 781 KPKRRTPVMYGGRSSSVPEKSQ-RSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLS 840
           KPKRR P  Y  R S+VP+KSQ RSRS++FSPDR RVRGRSPAFNALAANFE  N RN S
Sbjct: 781 KPKRRVPA-YSSR-STVPDKSQPRSRSMTFSPDRARVRGRSPAFNALAANFEKLNIRNQS 840

Query: 841 TPP----PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRS 900
           TPP    PMVR+LYPKS  PD S++A K +AIAA +A FE+P P  +   P S   +  +
Sbjct: 841 TPPPMVSPMVRKLYPKSHAPDLSKIAPK-SAIAARTALFEKPTPTSQE-PPTSPSSSEAT 900

Query: 901 LDASKPKPESD-NKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPD 960
             A  PK  S+ N+E +MS      +I ED KE EAE+E  L   PYE L T S +P  D
Sbjct: 901 NQAEAPKSTSETNEEEAMS------SINEDSKEEEAEEESSLPTFPYERLKTDSEDPVSD 960

Query: 961 IDITKREAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           +D+T+REAYL+S EF+EKF M K+ FYKLPKWKQNKLKM+++LF
Sbjct: 961 VDLTRREAYLTSVEFKEKFEMTKNEFYKLPKWKQNKLKMSVNLF 962

BLAST of MS002230 vs. ExPASy Swiss-Prot
Match: B8ATT7 (Villin-4 OS=Oryza sativa subsp. indica OX=39946 GN=VLN4 PE=3 SV=1)

HSP 1 Score: 1215.7 bits (3144), Expect = 0.0e+00
Identity = 611/999 (61.16%), Postives = 757/999 (75.78%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           M++SM+D+D AF+G GQK GLEVWRIENF+PV VP SSHGKF+ GDSY++LKT++LK+G+
Sbjct: 1   MSISMKDVDPAFRGVGQKDGLEVWRIENFKPVPVPTSSHGKFYMGDSYIILKTTALKNGS 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
            RHD+HYWLGKDT+QDEAG AAI TVELDAALGGRAVQYREVQG ETEK LSYF+PCI+P
Sbjct: 61  FRHDLHYWLGKDTSQDEAGTAAILTVELDAALGGRAVQYREVQGGETEKLLSYFRPCIMP 120

Query: 121 QEGGASSGFKHVEAD--EHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGL 180
           Q GG +SGF HVE +  +H  RLYVC+GK VVHVKE                        
Sbjct: 121 QPGGVASGFNHVEVNQQDHVTRLYVCQGKHVVHVKE------------------------ 180

Query: 181 LSCLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDG 240
                   V F RSSLNH+DIFILDT +KIFQFNGSNS IQERAKALEVVQYIKDT+H+G
Sbjct: 181 --------VPFVRSSLNHEDIFILDTANKIFQFNGSNSCIQERAKALEVVQYIKDTFHEG 240

Query: 241 KCEIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEP 300
           KCE+A +EDGKLMAD E GEFWGLFGGFAPLP+K + + +       TKLLC  +G  E 
Sbjct: 241 KCEVAAVEDGKLMADTEAGEFWGLFGGFAPLPKKTSSEDNGDDKETVTKLLCFNQGTLEH 300

Query: 301 VEADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIM 360
           +  +SL  ELLETNKCY++DCG E+++WMGR TSL  RK AS AAE+L+   +R  S+++
Sbjct: 301 ISFESLEHELLETNKCYLLDCGAEMYVWMGRGTSLQVRKGASEAAEKLLIDENRKGSNVI 360

Query: 361 RVIEAFEPIIFRAKFDSWPETAAVAV-SEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQ 420
           +VIE FE I+F++KF+ WP T  + + SEDGRGKVAALL+ QG++VKGL+KA P +EEPQ
Sbjct: 361 KVIEGFETIMFKSKFNKWPPTPDLKLSSEDGRGKVAALLRSQGLDVKGLMKAAPEEEEPQ 420

Query: 421 PYIDCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQS 480
           PYIDCTG+LQVWRV+G  K LL  SDQSK YTGDCYIFQY+Y G+D+EE LIGTWFGK+S
Sbjct: 421 PYIDCTGHLQVWRVNGDGKTLLSSSDQSKLYTGDCYIFQYTYTGDDKEECLIGTWFGKKS 480

Query: 481 VEEERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYIT 540
           VEE+RTSA+S+A KM ++ KF   QAR+YEG EPIQF+ IFQS  V+KGGLS GYKN+I 
Sbjct: 481 VEEDRTSAISLASKMFQAAKFQAAQARLYEGKEPIQFFVIFQSLQVFKGGLSSGYKNFIA 540

Query: 541 ENEIPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSL 600
            N   D T VE G+ALFRIQGSG +NMQAIQV AV SSLNSSYCYILH+G+TVFTW G+L
Sbjct: 541 VNGTDDDTYVEGGLALFRIQGSGSENMQAIQVDAVSSSLNSSYCYILHNGNTVFTWTGNL 600

Query: 601 TTSDDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLF 660
           TTS D +L+ER LDVIKP+  SR  KEG E++ FW LLGGK +Y ++KI + NESDPHLF
Sbjct: 601 TTSLDNDLVERQLDVIKPDLPSRSQKEGRETDQFWELLGGKCKYSNKKIGKENESDPHLF 660

Query: 661 SCTFAK---GVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFL 720
           SC  +K    V EI++F QDDLM EDI +L C +++FVWVGQ+VD K +  A++IGEKFL
Sbjct: 661 SCILSKENLKVKEIHHFTQDDLMAEDIFVLDCRTDLFVWVGQEVDAKLRSQAMDIGEKFL 720

Query: 721 EIDFLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTV 780
             DFL+E LS++ PI+IV EG+EP FFTRFF+WDSAKS MHG+S+QRKLA+V+ G TP++
Sbjct: 721 LHDFLMENLSQDTPIFIVTEGSEPQFFTRFFTWDSAKSLMHGSSYQRKLAIVKGGATPSL 780

Query: 781 DKPKRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLS 840
           DKPKRRTP  + GR++   +  QR+RS+S SP+R R+RGRSPAF A+A+ FENP+ R LS
Sbjct: 781 DKPKRRTPA-FSGRNAGQDKSQQRTRSMSHSPERHRIRGRSPAFTAIASAFENPSTRYLS 840

Query: 841 TPPPMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDAS 900
           TPPP V++L+P+S   +  + +SK +AI AL+++FE P    ++ IP+S+          
Sbjct: 841 TPPPAVKKLFPRSGGSELPKTSSKQSAINALTSAFEGP---TKSTIPKSV---------- 900

Query: 901 KPKPESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITK 960
           K  PE++      + + E  TI E   E E ED+E  TI+PYE LTT S +PAPDID+TK
Sbjct: 901 KVSPEAEK-----AIQEEGSTIGE--SENEPEDDENSTIYPYERLTTTSDDPAPDIDVTK 946

Query: 961 REAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           RE YLSS EF EKFGM + +F  LPKWKQN+LK  L LF
Sbjct: 961 REVYLSSVEFAEKFGMTRASFKNLPKWKQNRLKSDLQLF 946

BLAST of MS002230 vs. ExPASy TrEMBL
Match: A0A6J1BZG4 (villin-4-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007125 PE=4 SV=1)

HSP 1 Score: 1889.8 bits (4894), Expect = 0.0e+00
Identity = 952/996 (95.58%), Postives = 954/996 (95.78%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA
Sbjct: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKE                          
Sbjct: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKE-------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 VAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC
Sbjct: 181 ------VAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVA DGDKTVDSHPTKLLCVVKGNTEPVE
Sbjct: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVASDGDKTVDSHPTKLLCVVKGNTEPVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
           ADSLTRELLETNKCYIIDCGTEVFIWMGR TSLDERKSASRAAEELVSGPDRPQSHIMRV
Sbjct: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRATSLDERKSASRAAEELVSGPDRPQSHIMRV 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420
           IE FEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI
Sbjct: 361 IEGFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420

Query: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480
           DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE
Sbjct: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480

Query: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540
           ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE
Sbjct: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540

Query: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600
           IPDVTNVEDGVALFRIQGSGPDNMQAIQV+AVGSSLNSSYCYILHSGSTVFTWCGSLTTS
Sbjct: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVEAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600

Query: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660
           DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT
Sbjct: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660

Query: 661 FAK---GVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 720
           FAK    VAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID
Sbjct: 661 FAKEDLKVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 720

Query: 721 FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780
           FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP
Sbjct: 721 FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780

Query: 781 KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840
           KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP
Sbjct: 781 KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840

Query: 841 PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDASKPK 900
           PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG   SLDASKPK
Sbjct: 841 PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG---SLDASKPK 900

Query: 901 PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA 960
           PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA
Sbjct: 901 PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA 960

Query: 961 YLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           YLSSSEFREKFGMAKDAF+KLPKWKQNKLKMALHLF
Sbjct: 961 YLSSSEFREKFGMAKDAFHKLPKWKQNKLKMALHLF 961

BLAST of MS002230 vs. ExPASy TrEMBL
Match: A0A6J1C1H9 (villin-4-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007125 PE=4 SV=1)

HSP 1 Score: 1830.8 bits (4741), Expect = 0.0e+00
Identity = 929/996 (93.27%), Postives = 931/996 (93.47%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA
Sbjct: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKE                          
Sbjct: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKE-------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 VAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC
Sbjct: 181 ------VAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVA DGDKTVDSHPTKLLCVVKGNTEPVE
Sbjct: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVASDGDKTVDSHPTKLLCVVKGNTEPVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
           ADSLTRELLETNKCYIIDCGTEVFIWMGR TSLDERKSASRAAEELVSGPDRPQSHIMRV
Sbjct: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRATSLDERKSASRAAEELVSGPDRPQSHIMRV 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420
           IE FEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI
Sbjct: 361 IEGFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420

Query: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480
           DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE
Sbjct: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480

Query: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540
           ERTSALSMARKMVESLKFLPVQ                       GGLSDGYKNYITENE
Sbjct: 481 ERTSALSMARKMVESLKFLPVQ-----------------------GGLSDGYKNYITENE 540

Query: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600
           IPDVTNVEDGVALFRIQGSGPDNMQAIQV+AVGSSLNSSYCYILHSGSTVFTWCGSLTTS
Sbjct: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVEAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600

Query: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660
           DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT
Sbjct: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660

Query: 661 FAK---GVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 720
           FAK    VAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID
Sbjct: 661 FAKEDLKVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 720

Query: 721 FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780
           FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP
Sbjct: 721 FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780

Query: 781 KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840
           KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP
Sbjct: 781 KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840

Query: 841 PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDASKPK 900
           PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG   SLDASKPK
Sbjct: 841 PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG---SLDASKPK 900

Query: 901 PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA 960
           PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA
Sbjct: 901 PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA 938

Query: 961 YLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           YLSSSEFREKFGMAKDAF+KLPKWKQNKLKMALHLF
Sbjct: 961 YLSSSEFREKFGMAKDAFHKLPKWKQNKLKMALHLF 938

BLAST of MS002230 vs. ExPASy TrEMBL
Match: A0A1S3BWL6 (LOW QUALITY PROTEIN: villin-4 OS=Cucumis melo OX=3656 GN=LOC103494249 PE=4 SV=1)

HSP 1 Score: 1781.9 bits (4614), Expect = 0.0e+00
Identity = 888/996 (89.16%), Postives = 921/996 (92.47%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFF GDSY+VLKT+SLKSGA
Sbjct: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFMGDSYIVLKTTSLKSGA 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           LRHDIHYWLGKDTTQDEAG AAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LRHDIHYWLGKDTTQDEAGTAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGG SSGFKH EA+EH+  LYVCKGKRVVHVKE                          
Sbjct: 121 QEGGVSSGFKHAEAEEHKTTLYVCKGKRVVHVKE-------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 V FARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC
Sbjct: 181 ------VPFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           E+A IEDGKLMADVETGEFWGLFGGFAPLPRKVAG+GDKTVDSHPTKLLCV KGN EPVE
Sbjct: 241 EVAAIEDGKLMADVETGEFWGLFGGFAPLPRKVAGEGDKTVDSHPTKLLCVEKGNREPVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
           ADSL RELLETNKCYI+D GTEVFIWMGR +SL+ERK+ASRAAEELVS PDRPQSHIMRV
Sbjct: 301 ADSLARELLETNKCYILDSGTEVFIWMGRNSSLEERKNASRAAEELVSVPDRPQSHIMRV 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420
           IE FEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQG+NVKGLLKAEPVKEEPQPYI
Sbjct: 361 IEGFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGINVKGLLKAEPVKEEPQPYI 420

Query: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480
           DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSY GED+EEFL+GTWFGKQSVE 
Sbjct: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYSGEDKEEFLVGTWFGKQSVEG 480

Query: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540
           ER +ALS+A KMVESLKFLPVQAR+YEG+EPIQFYSIFQSFIV+KGGLSDGYKNYITENE
Sbjct: 481 ERAAALSLASKMVESLKFLPVQARLYEGHEPIQFYSIFQSFIVFKGGLSDGYKNYITENE 540

Query: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600
           IPDVTN EDGVALFR+QGSGP+NMQAIQV AVGSSLNSSYCYILHSGSTVFTWCGSLT +
Sbjct: 541 IPDVTNSEDGVALFRVQGSGPENMQAIQVDAVGSSLNSSYCYILHSGSTVFTWCGSLTNT 600

Query: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660
           DDQEL+ERFLDVIKPNCQS+PHKEGAESE FW LLGGKVEYPSQKI+RNNESDPHLFSCT
Sbjct: 601 DDQELVERFLDVIKPNCQSKPHKEGAESEQFWDLLGGKVEYPSQKIARNNESDPHLFSCT 660

Query: 661 FAK---GVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 720
           FA     VAEIYNFGQDDLMTEDIDILSCHS+IFVWVGQQVDPKTKVHAL IGEKFLEID
Sbjct: 661 FANEILKVAEIYNFGQDDLMTEDIDILSCHSDIFVWVGQQVDPKTKVHALKIGEKFLEID 720

Query: 721 FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780
           F LEKLSRE PIYIVMEG+EPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP
Sbjct: 721 FXLEKLSRETPIYIVMEGSEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVDKP 780

Query: 781 KRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840
           KRR PV+YGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP
Sbjct: 781 KRRAPVIYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPP 840

Query: 841 PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDASKPK 900
           PMVR+LYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG+L SL   KPK
Sbjct: 841 PMVRKLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGSLGSL---KPK 900

Query: 901 PESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKREA 960
           PESDNKENSMSNR+ESLTI EDVKEGE EDEEGLTIHPYESLTT SSNP  +ID+TKRE 
Sbjct: 901 PESDNKENSMSNRIESLTIAEDVKEGEVEDEEGLTIHPYESLTTNSSNPVSEIDVTKRET 960

Query: 961 YLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           YLSS+EFREKFGMAKDAFYKLPKWKQNKLKMALHLF
Sbjct: 961 YLSSAEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 961

BLAST of MS002230 vs. ExPASy TrEMBL
Match: A0A5D3CKI5 (Villin-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00250 PE=4 SV=1)

HSP 1 Score: 1769.2 bits (4581), Expect = 0.0e+00
Identity = 883/998 (88.48%), Postives = 918/998 (91.98%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFF GDSY+VLKT+SLKSGA
Sbjct: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFMGDSYIVLKTTSLKSGA 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           LRHDIHYWLGKDTTQDEAG AAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LRHDIHYWLGKDTTQDEAGTAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGG SSGFKH EA+EH+  LYVCKGKRVVH                             
Sbjct: 121 QEGGVSSGFKHAEAEEHKTTLYVCKGKRVVH----------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 V FARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC
Sbjct: 181 ------VPFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           E+A IEDGKLMADVETGEFWGLFGGFAPLPRKVAG+GDKTVDSHPTKLLCV KGN EPVE
Sbjct: 241 EVAAIEDGKLMADVETGEFWGLFGGFAPLPRKVAGEGDKTVDSHPTKLLCVEKGNREPVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
           ADSL RELLETNKCYI+D GTEVFIWMGR +SL+ERK+ASRAAEELVS PDRPQSHIMRV
Sbjct: 301 ADSLARELLETNKCYILDSGTEVFIWMGRNSSLEERKNASRAAEELVSVPDRPQSHIMRV 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420
           IE FEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQG+NVKGLLKAEPVKEEPQPYI
Sbjct: 361 IEGFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGINVKGLLKAEPVKEEPQPYI 420

Query: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480
           DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSY GED+EEFL+GTWFGKQSVE 
Sbjct: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYSGEDKEEFLVGTWFGKQSVEG 480

Query: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540
           ER +ALS+A KMVESLKFLPVQAR+YEG+EPIQFYSIFQSFIV+KGGLSDGYKNYITENE
Sbjct: 481 ERAAALSLASKMVESLKFLPVQARLYEGHEPIQFYSIFQSFIVFKGGLSDGYKNYITENE 540

Query: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600
           IPDVTN EDGVALFR+QGSGP+NMQAIQV AVGSSLNSSYCYILHSGSTVFTWCGSLT +
Sbjct: 541 IPDVTNSEDGVALFRVQGSGPENMQAIQVDAVGSSLNSSYCYILHSGSTVFTWCGSLTNT 600

Query: 601 DDQELLERFLDVIK-PNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSC 660
           DDQEL+ERFLDVIK PNCQS+PHKEGAESE FW LLGGKVEYPSQKI+RNNESDPHLFSC
Sbjct: 601 DDQELVERFLDVIKVPNCQSKPHKEGAESEQFWDLLGGKVEYPSQKIARNNESDPHLFSC 660

Query: 661 TFAKG----VAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLE 720
           TFA G    ++ IYNFGQDDLMTEDIDILSCHS+IFVWVGQQVDPKTKVHAL IGEKFLE
Sbjct: 661 TFANGAYNSISIIYNFGQDDLMTEDIDILSCHSDIFVWVGQQVDPKTKVHALKIGEKFLE 720

Query: 721 IDFLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVD 780
           IDF LEKLSRE PIYIVMEG+EPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVD
Sbjct: 721 IDFFLEKLSRETPIYIVMEGSEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVD 780

Query: 781 KPKRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLST 840
           KPKRR PV+YGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLST
Sbjct: 781 KPKRRAPVIYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLST 840

Query: 841 PPPMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDASK 900
           PPPMVR+LYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG+L SL   K
Sbjct: 841 PPPMVRKLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGSLGSL---K 900

Query: 901 PKPESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDIDITKR 960
           PKPESDNKENSMSNR+ESLTI EDVKEGE EDEEGLTIHPYESLTT SSNP  +ID+TKR
Sbjct: 901 PKPESDNKENSMSNRIESLTIAEDVKEGEVEDEEGLTIHPYESLTTNSSNPVSEIDVTKR 960

Query: 961 EAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           E YLSS+EFREKFGMAKDAFYKLPKWKQNKLKMALHLF
Sbjct: 961 ETYLSSAEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 960

BLAST of MS002230 vs. ExPASy TrEMBL
Match: A0A5A7USZ6 (Villin-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G005850 PE=4 SV=1)

HSP 1 Score: 1767.3 bits (4576), Expect = 0.0e+00
Identity = 883/1003 (88.04%), Postives = 918/1003 (91.53%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLK------TS 60
           MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFF GDSY+VLK      T+
Sbjct: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFMGDSYIVLKASGFGLTT 60

Query: 61  SLKSGALRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYF 120
           SLKSGALRHDIHYWLGKDTTQDEAG AAIKTVELDAALGGRAVQYREVQGHETEKFLSYF
Sbjct: 61  SLKSGALRHDIHYWLGKDTTQDEAGTAAIKTVELDAALGGRAVQYREVQGHETEKFLSYF 120

Query: 121 KPCIIPQEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFC 180
           KPCIIPQEGG SSGFKH EA+EH+  LYVCKGKRVVH                       
Sbjct: 121 KPCIIPQEGGVSSGFKHAEAEEHKTTLYVCKGKRVVH----------------------- 180

Query: 181 FFGLLSCLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDT 240
                       V FARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDT
Sbjct: 181 ------------VPFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDT 240

Query: 241 YHDGKCEIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKG 300
           YHDGKCE+A IEDGKLMADVETGEFWGLFGGFAPLPRKVAG+GDKTVDSHPTKLLCV KG
Sbjct: 241 YHDGKCEVAAIEDGKLMADVETGEFWGLFGGFAPLPRKVAGEGDKTVDSHPTKLLCVEKG 300

Query: 301 NTEPVEADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQ 360
           N EPVEADSL RELLETNKCYI+D GTEVFIWMGR +SL+ERK+ASRAAEELVS PDRPQ
Sbjct: 301 NREPVEADSLARELLETNKCYILDSGTEVFIWMGRNSSLEERKNASRAAEELVSVPDRPQ 360

Query: 361 SHIMRVIEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKE 420
           SHIMRVIE FEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQG+NVKGLLKAEPVKE
Sbjct: 361 SHIMRVIEGFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGINVKGLLKAEPVKE 420

Query: 421 EPQPYIDCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFG 480
           EPQPYIDCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSY GED+EEFL+GTWFG
Sbjct: 421 EPQPYIDCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYSGEDKEEFLVGTWFG 480

Query: 481 KQSVEEERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKN 540
           KQSVE ER +ALS+A KMVESLKFLPVQAR+YEG+EPIQFYSIFQSFIV+KGGLSDGYKN
Sbjct: 481 KQSVEGERAAALSLASKMVESLKFLPVQARLYEGHEPIQFYSIFQSFIVFKGGLSDGYKN 540

Query: 541 YITENEIPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWC 600
           YITENEIPDVTN EDGVALFR+QGSGP+NMQAIQV AVGSSLNSSYCYILHSGSTVFTWC
Sbjct: 541 YITENEIPDVTNSEDGVALFRVQGSGPENMQAIQVDAVGSSLNSSYCYILHSGSTVFTWC 600

Query: 601 GSLTTSDDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDP 660
           GSLT +DDQEL+ERFLDVIKPNCQS+PHKEGAESE FW LLGGKVEYPSQKI+RNNESDP
Sbjct: 601 GSLTNTDDQELVERFLDVIKPNCQSKPHKEGAESEQFWDLLGGKVEYPSQKIARNNESDP 660

Query: 661 HLFSCTFAKG----VAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIG 720
           HLFSCTFA G    ++ IYNFGQDDLMTEDIDILSCHS+IFVWVGQQVDPKTKVHAL IG
Sbjct: 661 HLFSCTFANGAYNSISIIYNFGQDDLMTEDIDILSCHSDIFVWVGQQVDPKTKVHALKIG 720

Query: 721 EKFLEIDFLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGG 780
           EKFLEIDF LEKLSRE PIYIVMEG+EPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGG
Sbjct: 721 EKFLEIDFFLEKLSRETPIYIVMEGSEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGG 780

Query: 781 TPTVDKPKRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNA 840
           TPTVDKPKRR PV+YGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNA
Sbjct: 781 TPTVDKPKRRAPVIYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENPNA 840

Query: 841 RNLSTPPPMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRS 900
           RNLSTPPPMVR+LYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRG+L S
Sbjct: 841 RNLSTPPPMVRKLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGSLGS 900

Query: 901 LDASKPKPESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPDI 960
           L   KPKPESDNKENSMSNR+ESLTI EDVKEGE EDEEGLTIHPYESLTT SSNP  +I
Sbjct: 901 L---KPKPESDNKENSMSNRIESLTIAEDVKEGEVEDEEGLTIHPYESLTTNSSNPVSEI 960

Query: 961 DITKREAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           D+TKRE YLSS+EFREKFGMAKDAFYKLPKWKQNKLKMALHLF
Sbjct: 961 DVTKRETYLSSAEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 965

BLAST of MS002230 vs. TAIR 10
Match: AT4G30160.1 (villin 4 )

HSP 1 Score: 1414.4 bits (3660), Expect = 0.0e+00
Identity = 708/1009 (70.17%), Postives = 821/1009 (81.37%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           M+VSMRDLD AFQGAGQKAG+E+WRIENF P  +PKSS GKFFTGDSY+VLKT++LK+GA
Sbjct: 1   MSVSMRDLDPAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGA 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           LRHDIHYWLGKDT+QDEAG AA+KTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LRHDIHYWLGKDTSQDEAGTAAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGG +SGFKHV A+EH  RL+VC+GK VVHVKE                          
Sbjct: 121 QEGGVASGFKHVVAEEHITRLFVCRGKHVVHVKE-------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 V FARSSLNHDDI+ILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDG C
Sbjct: 181 ------VPFARSSLNHDDIYILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGTC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           E+AT+EDGKLMAD ++GEFWG FGGFAPLPRK A D DKT +S  T+L CV KG   PVE
Sbjct: 241 EVATVEDGKLMADADSGEFWGFFGGFAPLPRKTANDEDKTYNSDITRLFCVEKGQANPVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
            D+L RE+L+TNKCYI+DCG EVF+WMGRTTSLD+RK AS+AAEE++   +RP+S ++R+
Sbjct: 301 GDTLKREMLDTNKCYILDCGIEVFVWMGRTTSLDDRKIASKAAEEMIRSSERPKSQMIRI 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420
           IE FE + FR+KF+SW +     VSEDGRG+VAALL+RQGVNV+GL+KA P KEEPQ +I
Sbjct: 361 IEGFETVPFRSKFESWTQETNTTVSEDGRGRVAALLQRQGVNVRGLMKAAPPKEEPQVFI 420

Query: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480
           DCTGNLQVWRV+GQ K LL  +D SKFY+GDCY+FQYSYPGE++EE LIGTWFGKQSVEE
Sbjct: 421 DCTGNLQVWRVNGQAKTLLQAADHSKFYSGDCYVFQYSYPGEEKEEVLIGTWFGKQSVEE 480

Query: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540
           ER SA+SMA KMVES+KF+P QARIYEG EPIQF+ I QSFIV+KGG+S GYK YI E E
Sbjct: 481 ERGSAVSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVFKGGISSGYKKYIAEKE 540

Query: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600
           + D T  E+GVALFRIQGSGP+NMQAIQV  V +SLNSSY YILH+ S+VFTW G+L+T+
Sbjct: 541 VDDDTYNENGVALFRIQGSGPENMQAIQVDPVAASLNSSYYYILHNDSSVFTWAGNLSTA 600

Query: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660
            DQEL ER LD+IKPN QSR  KEG+ESE FW LLGGK EY SQK+++  E DPHLFSCT
Sbjct: 601 TDQELAERQLDLIKPNQQSRAQKEGSESEQFWELLGGKAEYSSQKLTKEPERDPHLFSCT 660

Query: 661 FAK---GVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 720
           F K    V EIYNF QDDLMTEDI I+ CHSEIFVWVGQ+V PK K+ AL IGEKF+E D
Sbjct: 661 FTKEVLKVTEIYNFTQDDLMTEDIFIIDCHSEIFVWVGQEVVPKNKLLALTIGEKFIEKD 720

Query: 721 FLLEKLSREAPIYIVMEGNEPPFFTRFF-SWDSAKSAMHGNSFQRKLALVRNGGTPTVDK 780
            LLEKLS EAPIY++MEG EP FFTRFF SWDS+KSAMHGNSFQRKL +V+NGGTP  DK
Sbjct: 721 SLLEKLSPEAPIYVIMEGGEPSFFTRFFTSWDSSKSAMHGNSFQRKLKIVKNGGTPVADK 780

Query: 781 PKRRTPVMYGGRSSSVPEKS-QRSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLST 840
           PKRRTP  YGGR +SVP+KS QRSRS+SFSPDRVRVRGRSPAFNALAA FE+ NARNLST
Sbjct: 781 PKRRTPASYGGR-ASVPDKSQQRSRSMSFSPDRVRVRGRSPAFNALAATFESQNARNLST 840

Query: 841 PPPMVRRLYPKSVTPDSSRL--ASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRSLDA 900
           PPP+VR+LYP+SVTPDSS+   A K++AIA+ SA FE+ PP +E  IP+ ++ + ++ ++
Sbjct: 841 PPPVVRKLYPRSVTPDSSKFAPAPKSSAIASRSALFEKIPP-QEPSIPKPVKASPKTPES 900

Query: 901 SKP---------KPESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSS 960
             P         K E+D +E SMS+R+ESLTI+ED KEG  EDEE L  HPY+ L T S+
Sbjct: 901 PAPESNSKEQEEKKENDKEEGSMSSRIESLTIQEDAKEG-VEDEEDLPAHPYDRLKTTST 960

Query: 961 NPAPDIDITKREAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           +P  DID+T+REAYLSS EF+EKFGM K+AFYKLPKWKQNK KMA+ LF
Sbjct: 961 DPVSDIDVTRREAYLSSEEFKEKFGMTKEAFYKLPKWKQNKFKMAVQLF 974

BLAST of MS002230 vs. TAIR 10
Match: AT4G30160.2 (villin 4 )

HSP 1 Score: 1411.0 bits (3651), Expect = 0.0e+00
Identity = 708/1018 (69.55%), Postives = 821/1018 (80.65%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           M+VSMRDLD AFQGAGQKAG+E+WRIENF P  +PKSS GKFFTGDSY+VLKT++LK+GA
Sbjct: 1   MSVSMRDLDPAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGA 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           LRHDIHYWLGKDT+QDEAG AA+KTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LRHDIHYWLGKDTSQDEAGTAAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGG +SGFKHV A+EH  RL+VC+GK VVHVKE                          
Sbjct: 121 QEGGVASGFKHVVAEEHITRLFVCRGKHVVHVKE-------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 V FARSSLNHDDI+ILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDG C
Sbjct: 181 ------VPFARSSLNHDDIYILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGTC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           E+AT+EDGKLMAD ++GEFWG FGGFAPLPRK A D DKT +S  T+L CV KG   PVE
Sbjct: 241 EVATVEDGKLMADADSGEFWGFFGGFAPLPRKTANDEDKTYNSDITRLFCVEKGQANPVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
            D+L RE+L+TNKCYI+DCG EVF+WMGRTTSLD+RK AS+AAEE++   +RP+S ++R+
Sbjct: 301 GDTLKREMLDTNKCYILDCGIEVFVWMGRTTSLDDRKIASKAAEEMIRSSERPKSQMIRI 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYI 420
           IE FE + FR+KF+SW +     VSEDGRG+VAALL+RQGVNV+GL+KA P KEEPQ +I
Sbjct: 361 IEGFETVPFRSKFESWTQETNTTVSEDGRGRVAALLQRQGVNVRGLMKAAPPKEEPQVFI 420

Query: 421 DCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSVEE 480
           DCTGNLQVWRV+GQ K LL  +D SKFY+GDCY+FQYSYPGE++EE LIGTWFGKQSVEE
Sbjct: 421 DCTGNLQVWRVNGQAKTLLQAADHSKFYSGDCYVFQYSYPGEEKEEVLIGTWFGKQSVEE 480

Query: 481 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 540
           ER SA+SMA KMVES+KF+P QARIYEG EPIQF+ I QSFIV+KGG+S GYK YI E E
Sbjct: 481 ERGSAVSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVFKGGISSGYKKYIAEKE 540

Query: 541 IPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 600
           + D T  E+GVALFRIQGSGP+NMQAIQV  V +SLNSSY YILH+ S+VFTW G+L+T+
Sbjct: 541 VDDDTYNENGVALFRIQGSGPENMQAIQVDPVAASLNSSYYYILHNDSSVFTWAGNLSTA 600

Query: 601 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 660
            DQEL ER LD+IKPN QSR  KEG+ESE FW LLGGK EY SQK+++  E DPHLFSCT
Sbjct: 601 TDQELAERQLDLIKPNQQSRAQKEGSESEQFWELLGGKAEYSSQKLTKEPERDPHLFSCT 660

Query: 661 FAKG------------VAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALN 720
           F K             V EIYNF QDDLMTEDI I+ CHSEIFVWVGQ+V PK K+ AL 
Sbjct: 661 FTKEVLKVRILLKSFFVTEIYNFTQDDLMTEDIFIIDCHSEIFVWVGQEVVPKNKLLALT 720

Query: 721 IGEKFLEIDFLLEKLSREAPIYIVMEGNEPPFFTRFF-SWDSAKSAMHGNSFQRKLALVR 780
           IGEKF+E D LLEKLS EAPIY++MEG EP FFTRFF SWDS+KSAMHGNSFQRKL +V+
Sbjct: 721 IGEKFIEKDSLLEKLSPEAPIYVIMEGGEPSFFTRFFTSWDSSKSAMHGNSFQRKLKIVK 780

Query: 781 NGGTPTVDKPKRRTPVMYGGRSSSVPEKS-QRSRSVSFSPDRVRVRGRSPAFNALAANFE 840
           NGGTP  DKPKRRTP  YGGR +SVP+KS QRSRS+SFSPDRVRVRGRSPAFNALAA FE
Sbjct: 781 NGGTPVADKPKRRTPASYGGR-ASVPDKSQQRSRSMSFSPDRVRVRGRSPAFNALAATFE 840

Query: 841 NPNARNLSTPPPMVRRLYPKSVTPDSSRL--ASKNAAIAALSASFEQPPPAREAIIPRSL 900
           + NARNLSTPPP+VR+LYP+SVTPDSS+   A K++AIA+ SA FE+ PP +E  IP+ +
Sbjct: 841 SQNARNLSTPPPVVRKLYPRSVTPDSSKFAPAPKSSAIASRSALFEKIPP-QEPSIPKPV 900

Query: 901 RGNLRSLDASKP---------KPESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHP 960
           + + ++ ++  P         K E+D +E SMS+R+ESLTI+ED KEG  EDEE L  HP
Sbjct: 901 KASPKTPESPAPESNSKEQEEKKENDKEEGSMSSRIESLTIQEDAKEG-VEDEEDLPAHP 960

Query: 961 YESLTTLSSNPAPDIDITKREAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           Y+ L T S++P  DID+T+REAYLSS EF+EKFGM K+AFYKLPKWKQNK KMA+ LF
Sbjct: 961 YDRLKTTSTDPVSDIDVTRREAYLSSEEFKEKFGMTKEAFYKLPKWKQNKFKMAVQLF 983

BLAST of MS002230 vs. TAIR 10
Match: AT5G57320.1 (villin, putative )

HSP 1 Score: 1304.7 bits (3375), Expect = 0.0e+00
Identity = 650/1004 (64.74%), Postives = 786/1004 (78.29%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           M  SMRDLDQA QGAGQK+G+E+WRIENF+PV VP+ SHGKFFTGDSY+VLKT++ +SG+
Sbjct: 1   MTFSMRDLDQALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGS 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
           L HDIHYWLGKD++QDEAG  A+ TVELD+ALGGRAVQYREVQGHETEKFLSYFKPCIIP
Sbjct: 61  LHHDIHYWLGKDSSQDEAGAVAVMTVELDSALGGRAVQYREVQGHETEKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
           QEGG +SGF HV+ +EHQ RLY+CKGK VV VKE                          
Sbjct: 121 QEGGVASGFNHVKPEEHQTRLYICKGKHVVRVKE-------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                 V F RS+LNH+D+FILDT+SKIFQF+GS SSIQERAKALEVVQYIKDTYHDGKC
Sbjct: 181 ------VPFVRSTLNHEDVFILDTESKIFQFSGSKSSIQERAKALEVVQYIKDTYHDGKC 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           +IA +EDG++MAD E GEFWGLFGGFAPLP+K A + D+T  S   KL  V KG T+ VE
Sbjct: 241 DIAAVEDGRMMADAEAGEFWGLFGGFAPLPKKPAVNDDETAASDGIKLFSVEKGQTDAVE 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQSHIMRV 360
           A+ LT+ELL+TNKCYI+DCG E+F+W GR+TS+D+RKSA+ AAEE     + P+S+++ V
Sbjct: 301 AECLTKELLDTNKCYILDCGLELFVWKGRSTSIDQRKSATEAAEEFFRSSEPPKSNLVSV 360

Query: 361 IEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLK--AEPVKEEPQP 420
           +E +E ++FR+KFDSWP ++ +A  + GRGKVAALL+RQGVNV+GL+K  +   K+EP+P
Sbjct: 361 MEGYETVMFRSKFDSWPASSTIAEPQQGRGKVAALLQRQGVNVQGLVKTSSSSSKDEPKP 420

Query: 421 YIDCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSYPGEDREEFLIGTWFGKQSV 480
           YID TGNLQVWR++ +EK+LL  ++QSKFY+GDCYI QYSYPGEDREE L+GTWFGKQSV
Sbjct: 421 YIDGTGNLQVWRINCEEKILLEAAEQSKFYSGDCYILQYSYPGEDREEHLVGTWFGKQSV 480

Query: 481 EEERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITE 540
           EE+R SA+S+A KMVES+KF+P QARI EG EPIQF+ I QSFI +KGG+SD +K YI E
Sbjct: 481 EEDRASAISLANKMVESMKFVPAQARINEGKEPIQFFVIMQSFITFKGGVSDAFKKYIAE 540

Query: 541 NEIPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLT 600
           N+IPD T   +GVALFR+QGSGP+NMQAIQ++A  + LNSS+CYILH  STVFTWCG+LT
Sbjct: 541 NDIPDTTYEAEGVALFRVQGSGPENMQAIQIEAASAGLNSSHCYILHGDSTVFTWCGNLT 600

Query: 601 TSDDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFS 660
           +S+DQEL+ER LD+IKPN  ++  KEG+ESE FW LLGGK EYPSQKI R+ ESDPHLFS
Sbjct: 601 SSEDQELMERMLDLIKPNEPTKAQKEGSESEQFWELLGGKSEYPSQKIKRDGESDPHLFS 660

Query: 661 CTFAK---GVAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLE 720
           CT+        EI+NF QDDLMTEDI IL CH+E+FVWVGQQVDPK K  AL+IGE FL+
Sbjct: 661 CTYTNESLKATEIFNFTQDDLMTEDIFILDCHTEVFVWVGQQVDPKKKPQALDIGENFLK 720

Query: 721 IDFLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALVRNGGTPTVD 780
            DFLLE L+ E PIYIV EGNEPPFFTRFF+WDS+KS MHG+SFQRKLA++ N G P +D
Sbjct: 721 HDFLLENLASETPIYIVTEGNEPPFFTRFFTWDSSKSGMHGDSFQRKLAILTNKGKPLLD 780

Query: 781 KPKRRTPVMYGGRSSSVPEKSQ-RSRSVSFSPDRVRVRGRSPAFNALAANFENPNARNLS 840
           KPKRR P  Y  R S+VP+KSQ RSRS++FSPDR RVRGRSPAFNALAANFE  N RN S
Sbjct: 781 KPKRRVPA-YSSR-STVPDKSQPRSRSMTFSPDRARVRGRSPAFNALAANFEKLNIRNQS 840

Query: 841 TPP----PMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNLRS 900
           TPP    PMVR+LYPKS  PD S++A K +AIAA +A FE+P P  +   P S   +  +
Sbjct: 841 TPPPMVSPMVRKLYPKSHAPDLSKIAPK-SAIAARTALFEKPTPTSQE-PPTSPSSSEAT 900

Query: 901 LDASKPKPESD-NKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAPD 960
             A  PK  S+ N+E +MS      +I ED KE EAE+E  L   PYE L T S +P  D
Sbjct: 901 NQAEAPKSTSETNEEEAMS------SINEDSKEEEAEEESSLPTFPYERLKTDSEDPVSD 960

Query: 961 IDITKREAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
           +D+T+REAYL+S EF+EKF M K+ FYKLPKWKQNKLKM+++LF
Sbjct: 961 VDLTRREAYLTSVEFKEKFEMTKNEFYKLPKWKQNKLKMSVNLF 962

BLAST of MS002230 vs. TAIR 10
Match: AT2G41740.1 (villin 2 )

HSP 1 Score: 879.4 bits (2271), Expect = 2.7e-255
Identity = 467/1022 (45.69%), Postives = 640/1022 (62.62%), Query Frame = 0

Query: 3   VSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGALR 62
           +S + LD AFQGAGQK G E+WRIENF  V VPKS HGKF+ GD+Y+VL+T+  K GA  
Sbjct: 1   MSTKVLDPAFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYL 60

Query: 63  HDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQE 122
            DIH+W+GKDT+QDEAG AA+KTVELDA LGGRAVQ+RE+QGHE++KFLSYFKPCIIP E
Sbjct: 61  FDIHFWIGKDTSQDEAGTAAVKTVELDAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLE 120

Query: 123 GGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLSCL 182
           GG +SGFK VE +  + RLY CKGKR + +K                             
Sbjct: 121 GGVASGFKTVEEEVFETRLYTCKGKRAIRLK----------------------------- 180

Query: 183 LWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCEI 242
              QV FARSSLNHDD+FILDT+ KI+QFNG+NS+IQERAKALEVVQY+KD YH+G C++
Sbjct: 181 ---QVPFARSSLNHDDVFILDTEEKIYQFNGANSNIQERAKALEVVQYLKDKYHEGTCDV 240

Query: 243 ATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVEAD 302
           A ++DGKL  + ++G FW LFGGFAP+ RKVA D D   +S P KL C+  G  EP++ D
Sbjct: 241 AIVDDGKLDTESDSGAFWVLFGGFAPIGRKVANDDDIVPESTPPKLYCITDGKMEPIDGD 300

Query: 303 SLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQ-SHIMRVI 362
            L++ +LE  KCY++DCG E++IW+GR T +DERK+AS++AEE ++  +RP+ +H+ RVI
Sbjct: 301 -LSKSMLENTKCYLLDCGAEIYIWVGRVTQVDERKAASQSAEEFLASENRPKATHVTRVI 360

Query: 363 EAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPYID 422
           + +E   F++ FDSWP  +A   +E+GRGKVAALLK+QGV +KG+ K+ PV E+  P ++
Sbjct: 361 QGYESHSFKSNFDSWPSGSATPGNEEGRGKVAALLKQQGVGLKGIAKSAPVNEDIPPLLE 420

Query: 423 CTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSY-PGEDREEFLIGTWFGKQSVEE 482
             G L+VW V+G+ K  LP  D  K Y+GDCY+  Y+Y  GE ++E+ +  WFGK+S+ E
Sbjct: 421 SGGKLEVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGERKDEYFLSCWFGKKSIPE 480

Query: 483 ERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITENE 542
           ++ +A+ +A  M  SLK  PVQ RIYEG EP QF ++FQ  +V KGGLS GYK+ + E+E
Sbjct: 481 DQDTAIRLANTMSNSLKGRPVQGRIYEGKEPPQFVALFQPMVVLKGGLSSGYKSSMGESE 540

Query: 543 IPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLTTS 602
             D T   + +AL ++ G+G  N +A+QV+ V +SLNS  C++L SG+++F W G+ +T 
Sbjct: 541 STDETYTPESIALVQVSGTGVHNNKAVQVETVATSLNSYECFLLQSGTSMFLWHGNQSTH 600

Query: 603 DDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFSCT 662
           +  EL  +  + +KP    +  KEG ES  FW  LGGK  + S+K S     DPHLFS  
Sbjct: 601 EQLELATKVAEFLKPGITLKHAKEGTESSTFWFALGGKQNFTSKKASSETIRDPHLFSFA 660

Query: 663 FAKG---VAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLEID 722
           F +G   V EIYNF QDDL+TEDI  L  H+E+FVWVGQ V+PK K     IG+K++++ 
Sbjct: 661 FNRGKFQVEEIYNFAQDDLLTEDIYFLDTHAEVFVWVGQCVEPKEKQTVFEIGQKYIDLA 720

Query: 723 FLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLALV----------R 782
             LE L  + PIY + EGNEP FFT +FSWD+ K+ + GNSFQ+K +L+           
Sbjct: 721 GSLEGLHPKVPIYKINEGNEPCFFTTYFSWDATKAIVQGNSFQKKASLLFGTHHVVEDKS 780

Query: 783 NGGTPTVDKPKRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVR-----VRGRSPAFNALA 842
           NGG   +   ++R   +    S+     S  +R    S DR+       R R+ A  AL+
Sbjct: 781 NGGNQGL---RQRAEALAALNSAF---NSSSNRPAYSSQDRLNESHDGPRQRAEALAALS 840

Query: 843 ANFENPNARNLSTPPPMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPR 902
           + F + ++   S PPP      P   +  S R A+  A    L A  ++ P        R
Sbjct: 841 SAFNSSSSSTKSPPPPR-----PVGTSQASQRAAAVAALSQVLVAENKKSPDTSPT--RR 900

Query: 903 SLRGNLRS---LDASKPKPESD-------NKENSMSNRLESLTIEEDVKE-GEAEDEEGL 962
           S   N      L  +K + E+         +E  +S   +    +++ +E G++E +   
Sbjct: 901 STSSNPADDIPLTEAKDEEEASEVAGLEAKEEEEVSPAADETEAKQETEEQGDSEIQPSG 960

Query: 963 TIHPYESLTTLSSNPAPDIDITKREAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALH 994
               YE L   S NP   ID  +REAYLS  EF+  FG+ K+AF  LP+WKQ+ LK    
Sbjct: 961 ATFTYEQLRAKSENPVTGIDFKRREAYLSEEEFQSVFGIEKEAFNNLPRWKQDLLKKKFD 976

BLAST of MS002230 vs. TAIR 10
Match: AT3G57410.1 (villin 3 )

HSP 1 Score: 875.2 bits (2260), Expect = 5.1e-254
Identity = 461/1005 (45.87%), Postives = 636/1005 (63.28%), Query Frame = 0

Query: 1   MAVSMRDLDQAFQGAGQKAGLEVWRIENFRPVLVPKSSHGKFFTGDSYVVLKTSSLKSGA 60
           M+ S + LD AFQG GQK G E+WRIENF PV VPKS HGKF+ GD+Y+VL+T+  K GA
Sbjct: 1   MSGSTKVLDPAFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGA 60

Query: 61  LRHDIHYWLGKDTTQDEAGVAAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIP 120
              DIH+W+GKDT+QDEAG AA+KTVELDAALGGRAVQYRE+QGHE++KFLSYFKPCIIP
Sbjct: 61  YLFDIHFWIGKDTSQDEAGTAAVKTVELDAALGGRAVQYREIQGHESDKFLSYFKPCIIP 120

Query: 121 QEGGASSGFKHVEADEHQIRLYVCKGKRVVHVKEASLTSDMQADWALASPFLFCFFGLLS 180
            EGG +SGFK  E +E + RLY CKGKR VH+K                           
Sbjct: 121 LEGGVASGFKKPEEEEFETRLYTCKGKRAVHLK--------------------------- 180

Query: 181 CLLWWQVAFARSSLNHDDIFILDTKSKIFQFNGSNSSIQERAKALEVVQYIKDTYHDGKC 240
                QV FARSSLNHDD+FILDTK KI+QFNG+NS+IQERAKAL V+QY+KD +H+G  
Sbjct: 181 -----QVPFARSSLNHDDVFILDTKEKIYQFNGANSNIQERAKALVVIQYLKDKFHEGTS 240

Query: 241 EIATIEDGKLMADVETGEFWGLFGGFAPLPRKVAGDGDKTVDSHPTKLLCVVKGNTEPVE 300
           ++A ++DGKL  + ++GEFW LFGGFAP+ RKVA + +   ++ P KL  +  G  E ++
Sbjct: 241 DVAIVDDGKLDTESDSGEFWVLFGGFAPIARKVASEDEIIPETTPPKLYSIADGQVESID 300

Query: 301 ADSLTRELLETNKCYIIDCGTEVFIWMGRTTSLDERKSASRAAEELVSGPDRPQ-SHIMR 360
            D L++ +LE NKCY++DCG+E+FIW+GR T ++ERK+A +AAE+ V+  +RP+ + I R
Sbjct: 301 GD-LSKSMLENNKCYLLDCGSEIFIWVGRVTQVEERKTAIQAAEDFVASENRPKATRITR 360

Query: 361 VIEAFEPIIFRAKFDSWPETAAVAVSEDGRGKVAALLKRQGVNVKGLLKAEPVKEEPQPY 420
           VI+ +EP  F++ FDSWP  +A   +E+GRGKVAALLK+QGV +KGL K+ PV E+  P 
Sbjct: 361 VIQGYEPHSFKSNFDSWPSGSATPANEEGRGKVAALLKQQGVGLKGLSKSTPVNEDIPPL 420

Query: 421 IDCTGNLQVWRVSGQEKLLLPVSDQSKFYTGDCYIFQYSY-PGEDREEFLIGTWFGKQSV 480
           ++  G L+VW +    K +L      K Y+GDCY+  Y+Y  GE +E++ +  WFGK S 
Sbjct: 421 LEGGGKLEVWYIDANSKTVLSKDHVGKLYSGDCYLVLYTYHSGERKEDYFLCCWFGKNSN 480

Query: 481 EEERTSALSMARKMVESLKFLPVQARIYEGYEPIQFYSIFQSFIVYKGGLSDGYKNYITE 540
           +E++ +A+ +A  M  SLK  PVQARI+EG EP QF ++FQ  +V KGGLS GYKN +TE
Sbjct: 481 QEDQETAVRLASTMTNSLKGRPVQARIFEGKEPPQFVALFQHMVVLKGGLSSGYKNSMTE 540

Query: 541 NEIPDVTNVEDGVALFRIQGSGPDNMQAIQVKAVGSSLNSSYCYILHSGSTVFTWCGSLT 600
                 T   + +AL ++ G+G  N +A+QV+AV +SLNS  C++L SG+++F W G+ +
Sbjct: 541 KGSSGETYTPESIALIQVSGTGVHNNKALQVEAVATSLNSYDCFLLQSGTSMFLWVGNHS 600

Query: 601 TSDDQELLERFLDVIKPNCQSRPHKEGAESEHFWGLLGGKVEYPSQKISRNNESDPHLFS 660
           T + QEL  +  + +KP    +  KEG ES  FW  LGGK  + S+K+S     DPHLFS
Sbjct: 601 THEQQELAAKVAEFLKPGTTIKHAKEGTESSSFWFALGGKQNFTSKKVSSETVRDPHLFS 660

Query: 661 CTFAKG---VAEIYNFGQDDLMTEDIDILSCHSEIFVWVGQQVDPKTKVHALNIGEKFLE 720
            +F +G   V EI+NF QDDL+TE++ +L  H+E+FVWVGQ VDPK K  A  IG++++ 
Sbjct: 661 FSFNRGKFQVEEIHNFDQDDLLTEEMHLLDTHAEVFVWVGQCVDPKEKQTAFEIGQRYIN 720

Query: 721 IDFLLEKLSREAPIYIVMEGNEPPFFTRFFSWDSAKSAMHGNSFQRKLAL-------VRN 780
           +   LE LS + P+Y + EGNEP FFT +FSWDS K+ + GNS+Q+K AL       V +
Sbjct: 721 LAGSLEGLSPKVPLYKITEGNEPCFFTTYFSWDSTKATVQGNSYQKKAALLLGTHHVVED 780

Query: 781 GGTPTVDKPKRRTPVMYGGRSSSVPEKSQRSRSVSFSPDRVRVRGRSPAFNALAANFENP 840
             +     P++R   +    +S+    S R+ S S         G      ALAA     
Sbjct: 781 QSSSGNQGPRQRAAAL-AALTSAFNSSSGRTSSPSRDRSNGSQGGPRQRAEALAALTSAF 840

Query: 841 NARNLSTPPPMVRRLYPKSVTPDSSRLASKNAAIAALSASFEQPPPAREAIIPRSLRGNL 900
           N+   S  PP  RR     +T  +S+ A+  AA++ +  + ++  P              
Sbjct: 841 NSSPSSKSPP--RR---SGLTSQASQRAAAVAALSQVLTAEKKKSPDTSPSAEAKDEKAF 900

Query: 901 RSLDASKPKPESDNKENSMSNRLESLTIEEDVKEGEAEDEEGLTIHPYESLTTLSSNPAP 960
             ++A++   E+  +E  +S   E+   E   K+ ++E E       YE L   S  P  
Sbjct: 901 SEVEATEEATEA-KEEEEVSPAAEASAEEAKPKQDDSEVETTGVTFTYERLQAKSEKPVT 960

Query: 961 DIDITKREAYLSSSEFREKFGMAKDAFYKLPKWKQNKLKMALHLF 994
            ID  +REAYLS  EF+  FGM K++FYKLP WKQ+ LK   +LF
Sbjct: 961 GIDFKRREAYLSEVEFKTVFGMEKESFYKLPGWKQDLLKKKFNLF 965

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135030.10.0e+0095.58villin-4-like isoform X1 [Momordica charantia] >XP_022135031.1 villin-4-like iso... [more]
XP_022135032.10.0e+0093.27villin-4-like isoform X2 [Momordica charantia][more]
XP_008453585.20.0e+0089.16PREDICTED: LOW QUALITY PROTEIN: villin-4 [Cucumis melo][more]
XP_038879573.10.0e+0088.86villin-4-like isoform X1 [Benincasa hispida] >XP_038879575.1 villin-4-like isofo... [more]
TYK11812.10.0e+0088.48villin-4 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
O655700.0e+0070.17Villin-4 OS=Arabidopsis thaliana OX=3702 GN=VLN4 PE=1 SV=1[more]
Q0J7160.0e+0065.53Villin-5 OS=Oryza sativa subsp. japonica OX=39947 GN=VLN5 PE=3 SV=1[more]
Q67U260.0e+0064.71Villin-3 OS=Oryza sativa subsp. japonica OX=39947 GN=VLN3 PE=2 SV=2[more]
Q9LVC60.0e+0064.74Villin-5 OS=Arabidopsis thaliana OX=3702 GN=VLN5 PE=2 SV=1[more]
B8ATT70.0e+0061.16Villin-4 OS=Oryza sativa subsp. indica OX=39946 GN=VLN4 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BZG40.0e+0095.58villin-4-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007125 PE=4 SV=... [more]
A0A6J1C1H90.0e+0093.27villin-4-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007125 PE=4 SV=... [more]
A0A1S3BWL60.0e+0089.16LOW QUALITY PROTEIN: villin-4 OS=Cucumis melo OX=3656 GN=LOC103494249 PE=4 SV=1[more]
A0A5D3CKI50.0e+0088.48Villin-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00250 PE=4 ... [more]
A0A5A7USZ60.0e+0088.04Villin-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G005850 PE=4... [more]
Match NameE-valueIdentityDescription
AT4G30160.10.0e+0070.17villin 4 [more]
AT4G30160.20.0e+0069.55villin 4 [more]
AT5G57320.10.0e+0064.74villin, putative [more]
AT2G41740.12.7e-25545.69villin 2 [more]
AT3G57410.15.1e-25445.87villin 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007122Villin/GelsolinPRINTSPR00597GELSOLINcoord: 501..521
score: 44.16
coord: 564..584
score: 41.34
coord: 443..459
score: 46.79
coord: 355..376
score: 30.17
coord: 468..486
score: 30.38
coord: 673..695
score: 50.0
coord: 702..721
score: 23.41
coord: 624..643
score: 52.5
IPR007122Villin/GelsolinSMARTSM00262VILL_6coord: 424..521
e-value: 2.0E-21
score: 87.2
coord: 286..376
e-value: 3.3E-16
score: 69.8
coord: 551..638
e-value: 1.1E-17
score: 74.7
coord: 655..747
e-value: 8.0E-11
score: 52.0
coord: 168..266
e-value: 2.0E-21
score: 87.2
coord: 19..117
e-value: 1.2E-29
score: 114.5
IPR007122Villin/GelsolinPANTHERPTHR11977VILLINcoord: 1..155
coord: 186..993
IPR003128Villin headpieceSMARTSM00153VHP_1coord: 958..993
e-value: 3.5E-17
score: 73.1
IPR003128Villin headpiecePFAMPF02209VHPcoord: 958..993
e-value: 5.4E-14
score: 52.0
IPR003128Villin headpiecePROSITEPS51089HPcoord: 928..993
score: 19.64686
IPR029006ADF-H/Gelsolin-like domain superfamilyGENE3D3.40.20.10Severincoord: 7..131
e-value: 1.3E-48
score: 166.0
IPR029006ADF-H/Gelsolin-like domain superfamilyGENE3D3.40.20.10Severincoord: 279..387
e-value: 7.6E-19
score: 69.7
IPR029006ADF-H/Gelsolin-like domain superfamilyGENE3D3.40.20.10Severincoord: 550..644
e-value: 2.1E-21
score: 77.9
IPR029006ADF-H/Gelsolin-like domain superfamilyGENE3D3.40.20.10Severincoord: 413..549
e-value: 6.7E-28
score: 99.2
IPR029006ADF-H/Gelsolin-like domain superfamilyGENE3D3.40.20.10Severincoord: 645..764
e-value: 1.7E-24
score: 88.1
IPR029006ADF-H/Gelsolin-like domain superfamilyGENE3D3.40.20.10Severincoord: 132..278
e-value: 2.2E-29
score: 103.8
IPR007123Gelsolin-like domainPFAMPF00626Gelsolincoord: 296..369
e-value: 4.5E-9
score: 36.1
coord: 30..111
e-value: 1.6E-15
score: 56.8
coord: 187..249
e-value: 1.8E-9
score: 37.3
IPR036886Villin headpiece domain superfamilyGENE3D1.10.950.10Villin headpiece domaincoord: 917..993
e-value: 6.7E-21
score: 76.1
IPR036886Villin headpiece domain superfamilySUPERFAMILY47050VHP, Villin headpiece domaincoord: 919..993
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 790..804
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 892..906
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 768..808
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 869..916
NoneNo IPR availablePANTHERPTHR11977:SF115VILLIN-3-LIKEcoord: 1..155
coord: 186..993
NoneNo IPR availableCDDcd11290gelsolin_S1_likecoord: 12..124
e-value: 1.23165E-66
score: 216.705
NoneNo IPR availableCDDcd11292gelsolin_S3_likecoord: 293..375
e-value: 3.13254E-27
score: 104.254
NoneNo IPR availableCDDcd11291gelsolin_S6_likecoord: 653..748
e-value: 2.26251E-36
score: 130.495
NoneNo IPR availableCDDcd11288gelsolin_S5_likecoord: 549..640
e-value: 1.18377E-30
score: 113.866
NoneNo IPR availableCDDcd11293gelsolin_S4_likecoord: 418..518
e-value: 3.51012E-41
score: 144.336
NoneNo IPR availableSUPERFAMILY55753Actin depolymerizing proteinscoord: 167..269
NoneNo IPR availableSUPERFAMILY55753Actin depolymerizing proteinscoord: 9..147
NoneNo IPR availableSUPERFAMILY55753Actin depolymerizing proteinscoord: 542..646
NoneNo IPR availableSUPERFAMILY55753Actin depolymerizing proteinscoord: 627..746
NoneNo IPR availableSUPERFAMILY55753Actin depolymerizing proteinscoord: 414..560
IPR036180Gelsolin-like domain superfamilySUPERFAMILY82754C-terminal, gelsolin-like domain of Sec23/24coord: 287..459

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS002230.1MS002230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007010 cytoskeleton organization
molecular_function GO:0003779 actin binding
molecular_function GO:0051015 actin filament binding