MS002197 (gene) Bitter gourd (TR) v1

BLAST of MS002197 vs. NCBI nr
Match: XP_023531143.1 (RING-H2 finger protein ATL43 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 543.9 bits (1400), Expect = 1.2e-150
Identity = 305/423 (72.10%), Postives = 329/423 (77.78%), Query Frame = 0

Query: 1   MGALPNPFSFFFFLLLLFFSMAAA----DQAPDLRFPRPFLYRFQALEGAVLVA----PP 60
           MG LP+ F F    L    S AAA    D + +L  P P LYRF+A++  V  A    PP
Sbjct: 1   MGTLPDSFLFSLIFLFTVVSPAAAALPGDPSLNLLLPHPILYRFKAIQETVFDASPSPPP 60

Query: 61  PPFASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNVVVVGYSRRP 120
           PP  S PPPE+KPVY+PFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN VVVGYSR P
Sbjct: 61  PPLPSPPPPEDKPVYSPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNAVVVGYSRYP 120

Query: 121 NS-MGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTEVLRL 180
           NS MG+   PSF+ RKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCL++FE TE+LRL
Sbjct: 121 NSVMGA---PSFTTRKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLDKFEQTEILRL 180

Query: 181 LPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNILVHNQAAASAGANQISP 240
           LPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLL+EDVNI ++N             
Sbjct: 181 LPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLIEDVNIFLYN------------- 240

Query: 241 PPPPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVSGRHSSVGEKRTGGFWQNLME 300
            P P PPP PPPRDS SKDVVLNLE GR+S GKA SGRVSGRHSSVGEK           
Sbjct: 241 -PAPAPPPLPPPRDSNSKDVVLNLEQGRKS-GKAGSGRVSGRHSSVGEK----------- 300

Query: 301 RTGESSSRDPALFRRSLDSSSAAPRKRTETVAVGCFDRPRKDGLLLPDEKPNLQNQNQNQ 360
           RTGESS RDPAL RRSLDS      KR+ETVA+GCFDR RKDGLLLPDEK N QNQNQNQ
Sbjct: 301 RTGESSRRDPALLRRSLDS------KRSETVAIGCFDRHRKDGLLLPDEKTN-QNQNQNQ 360

Query: 361 NRFEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMIISESRRYSAASLPPELKRQRR 415
           +RFEHRIIVSPR       +QRWSDV ASDLLYLRSEMIIS+SRR+S ASLPPELKRQRR
Sbjct: 361 SRFEHRIIVSPRAPVVVQGQQRWSDVHASDLLYLRSEMIISDSRRFSVASLPPELKRQRR 387

BLAST of MS002197 vs. NCBI nr
Match: XP_038879064.1 (RING-H2 finger protein ATL43 [Benincasa hispida])

HSP 1 Score: 542.7 bits (1397), Expect = 2.7e-150
Identity = 309/423 (73.05%), Postives = 322/423 (76.12%), Query Frame = 0

Query: 1   MGALPNPFSFF--FFLLLLFFSMAAADQAPDLR--FPRPFLYRFQALEGAVLVA-----P 60
           MGALPN FS F   FL       AA    P L    P PFLYRF++L+  + VA     P
Sbjct: 1   MGALPNSFSLFSLIFLFTAVSPAAATAGGPPLNLILPHPFLYRFKSLKETLFVASPSPPP 60

Query: 61  PPPFASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNVVVVGYSRR 120
           PP F SAPPPEEKPVY+PFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN VV+GYS R
Sbjct: 61  PPSFPSAPPPEEKPVYSPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNAVVIGYSMR 120

Query: 121 PNSMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTEVLRL 180
           PN+M     PSFS RKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEP EVLRL
Sbjct: 121 PNTMMG--VPSFSTRKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEPAEVLRL 180

Query: 181 LPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNILVHNQAAASAGANQISP 240
           LPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNI +HN             
Sbjct: 181 LPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNIFLHN------------- 240

Query: 241 PPPPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVSGRHSSVGEKRTGGFWQNLME 300
                 PPP PPR+S SKDVVLNLE GRRS  KA SGRVSGRHSSVGEK           
Sbjct: 241 ------PPPLPPRESNSKDVVLNLEQGRRS-SKAGSGRVSGRHSSVGEK----------- 300

Query: 301 RTGESSSRDPALFRRSLDSSSAAPRKRTETVAVGCFDRPRKDGLLLPDEKPNLQNQNQNQ 360
           RTGESS RDPAL RRSLDS      KRTETVAVGCFDR RKDGLLLP+EK    NQNQNQ
Sbjct: 301 RTGESSYRDPALLRRSLDS------KRTETVAVGCFDRHRKDGLLLPEEK---TNQNQNQ 360

Query: 361 NRFEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMIISESRRYSAASLPPELKRQRR 415
           NR EHRIIVSPR       +Q WSDVQASDLLYLRSEMIIS+SRR+S ASLPPELKRQRR
Sbjct: 361 NRLEHRIIVSPRVPVMVQGQQTWSDVQASDLLYLRSEMIISDSRRFSVASLPPELKRQRR 381

BLAST of MS002197 vs. NCBI nr
Match: KAG7021678.1 (RING-H2 finger protein ATL43, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 540.4 bits (1391), Expect = 1.3e-149
Identity = 302/423 (71.39%), Postives = 326/423 (77.07%), Query Frame = 0

Query: 1   MGALPNPFSFFFFLLLLFFSMAAA-----DQAPDLRFPRPFLYRFQALEGAVLVA----P 60
           MG LP+ F F    L    S AAA     D + +L  P P LYRF+A++  V  A    P
Sbjct: 1   MGTLPDSFLFSLIFLFTVVSPAAAAALPGDPSLNLLLPHPILYRFKAIQETVFDASPSPP 60

Query: 61  PPPFASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNVVVVGYSRR 120
           PPP  S PPPE+KPVY+PFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN VVVGYSR 
Sbjct: 61  PPPLPSPPPPEDKPVYSPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNAVVVGYSRY 120

Query: 121 PNSMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTEVLRL 180
           PNS+     PSF+ RKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCL++FE TE+LRL
Sbjct: 121 PNSVIGA--PSFTTRKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLDKFEQTEILRL 180

Query: 181 LPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNILVHNQAAASAGANQISP 240
           LPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLL+EDVNI ++N             
Sbjct: 181 LPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLIEDVNIFLYN------------- 240

Query: 241 PPPPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVSGRHSSVGEKRTGGFWQNLME 300
            P P PPP PPPRDS SKDVVLNLE GR+S GKA SGRVSGRHSSVGEK           
Sbjct: 241 -PAPAPPPMPPPRDSNSKDVVLNLEQGRKS-GKAGSGRVSGRHSSVGEK----------- 300

Query: 301 RTGESSSRDPALFRRSLDSSSAAPRKRTETVAVGCFDRPRKDGLLLPDEKPNLQNQNQNQ 360
           RTGESS RDPAL RRSLDS      K +ETVA+GCFDR RKDGLLLPDEK N QNQNQNQ
Sbjct: 301 RTGESSRRDPALLRRSLDS------KSSETVAIGCFDRHRKDGLLLPDEKTN-QNQNQNQ 360

Query: 361 NRFEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMIISESRRYSAASLPPELKRQRR 415
           +RFEHRIIVSPR       +QRWSDV ASDLLYLRSEMIIS+SRR+S ASLPPELKRQRR
Sbjct: 361 SRFEHRIIVSPRAPVVVQGQQRWSDVHASDLLYLRSEMIISDSRRFSVASLPPELKRQRR 388

BLAST of MS002197 vs. NCBI nr
Match: KAG6587710.1 (RING-H2 finger protein ATL43, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 532.7 bits (1371), Expect = 2.8e-147
Identity = 291/394 (73.86%), Postives = 314/394 (79.70%), Query Frame = 0

Query: 25  DQAPDLRFPRPFLYRFQALEGAVLVA----PPPPFASAPPPEEKPVYAPFRPSMAVVVGV 84
           D + +L  P P LYRF+A++  V  A    PPPP  S PPPE+KPVY+PFRPSMAVVVGV
Sbjct: 43  DPSLNLLLPHPILYRFKAIQETVFDASPSPPPPPLPSPPPPEDKPVYSPFRPSMAVVVGV 102

Query: 85  LTTTFSITFLLLLYAKHCKRGNVVVVGYSRRPNSMGSGPNPSFSARKNSGIDQAVIESLP 144
           LTTTFSITFLLLLYAKHCKRGN VVVGYSR PNS+     PSF+ RKNSGIDQAVIESLP
Sbjct: 103 LTTTFSITFLLLLYAKHCKRGNAVVVGYSRYPNSVIGA--PSFTTRKNSGIDQAVIESLP 162

Query: 145 IFRFGSLSGQKEGLECAVCLNRFEPTEVLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYR 204
           IFRFGSLSGQKEGLECAVCL++FE TE+LRLLPKCKHAFHVECVDTWLDAHSTCPLCRYR
Sbjct: 163 IFRFGSLSGQKEGLECAVCLDKFEQTEILRLLPKCKHAFHVECVDTWLDAHSTCPLCRYR 222

Query: 205 VDPEDVLLVEDVNILVHNQAAASAGANQISPPPPPLPPPPPPPRDSKSKDVVLNLEPGRR 264
           VDPEDVLL+EDVNI ++N              P P PPP PPPRDS SKDVVLNLE GR+
Sbjct: 223 VDPEDVLLIEDVNIFLYN--------------PAPAPPPMPPPRDSNSKDVVLNLEQGRK 282

Query: 265 SGGKAESGRVSGRHSSVGEKRTGGFWQNLMERTGESSSRDPALFRRSLDSSSAAPRKRTE 324
           S GKA SGRVSGRHSSVGEK           RTGESS RDPAL RRSLDS      K +E
Sbjct: 283 S-GKAGSGRVSGRHSSVGEK-----------RTGESSRRDPALLRRSLDS------KSSE 342

Query: 325 TVAVGCFDRPRKDGLLLPDEKPNLQNQNQNQNRFEHRIIVSPRGRAGEAQRQRWSDVQAS 384
           TVA+GCFDR RKDGLLLPDEK N QNQNQNQ+RFEHRIIVSPR       +QRWSDV AS
Sbjct: 343 TVAIGCFDRHRKDGLLLPDEKTN-QNQNQNQSRFEHRIIVSPRAPVVVQGQQRWSDVHAS 401

Query: 385 DLLYLRSEMIISESRRYSAASLPPELKRQRRMGM 415
           DLLYLRSEMIIS+SRR+S ASLPPELKRQRRMG+
Sbjct: 403 DLLYLRSEMIISDSRRFSVASLPPELKRQRRMGI 401

BLAST of MS002197 vs. NCBI nr
Match: TYK28550.1 (RING-H2 finger protein ATL43 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 532.3 bits (1370), Expect = 3.6e-147
Identity = 311/427 (72.83%), Postives = 323/427 (75.64%), Query Frame = 0

Query: 1   MGALPNPFSFFFFLLLLFFSMAAADQAPDLRF----------PRPFLYRFQALEGAVLVA 60
           MG LPN FS  F L  LF +++ A    DLR              FL    +   +    
Sbjct: 1   MGTLPNSFS-LFSLFFLFIAVSPAAPTTDLRSLNLILTHHLKETVFLASSSSSSSSSSSP 60

Query: 61  PPPPF-ASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN-VVVVGY 120
           PPPPF  SAPPPEEKPVY+PFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN  VVVGY
Sbjct: 61  PPPPFIPSAPPPEEKPVYSPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNGAVVVGY 120

Query: 121 SRRPN-SMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTE 180
           S RPN +MG    PSFS RKNSGIDQ VIESLPIFRFGSLSGQKEGLECAVCLNRFEPTE
Sbjct: 121 SMRPNTTMGV---PSFSTRKNSGIDQTVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTE 180

Query: 181 VLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNILVHNQAAASAGAN 240
           VLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNI +HNQ        
Sbjct: 181 VLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNIFLHNQ-------- 240

Query: 241 QISPPPPPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVSGRHSSVGEKRTGGFWQ 300
                 PP PPPPPPPR+S SKDVVLNLE GRRS GKA SGRVSGRHSSVGEK       
Sbjct: 241 ------PPPPPPPPPPRESNSKDVVLNLEQGRRS-GKAGSGRVSGRHSSVGEK------- 300

Query: 301 NLMERTGESSSRDPALFRRSLDSSSAAPRKRTETVAVGCFDRPRKDGLLLPDEKPNLQNQ 360
               RTGESS RDPAL RRSLDS      KRTETV+VGCFDR RKDGLLLP+EK N QNQ
Sbjct: 301 ----RTGESSYRDPALLRRSLDS------KRTETVSVGCFDRHRKDGLLLPEEKSN-QNQ 360

Query: 361 NQNQNRFEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMIISESRRYSAASLPPELK 415
           NQNQNR EHRIIVSPR    E    RWSDVQ SDLLYLRSEMIIS+SRRYS ASLP ELK
Sbjct: 361 NQNQNRLEHRIIVSPRVPVVE----RWSDVQGSDLLYLRSEMIISDSRRYSVASLPVELK 386

BLAST of MS002197 vs. ExPASy Swiss-Prot
Match: Q5EAE9 (RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2)

HSP 1 Score: 286.6 bits (732), Expect = 4.6e-76
Identity = 183/360 (50.83%), Postives = 219/360 (60.83%), Query Frame = 0

Query: 52  PPPFASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN-VVVVGYSR 111
           PPP     PP      +   P +AVV+ VLT  FS+TFLLLLY KHCKR N  V V + +
Sbjct: 36  PPPLPPPSPPPRHNFTSSLMPGIAVVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQ 95

Query: 112 R--PNSMGSG---PNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEP 171
           R      G G          RKNSGID++VIESLP+FRFG+LSG K+GLECAVCL RFEP
Sbjct: 96  RFAITRYGGGYYNGGGVVGGRKNSGIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEP 155

Query: 172 TEVLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNILVHNQAAASAG 231
           TEVLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPED+LL+ D N     Q +    
Sbjct: 156 TEVLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDILLIGDCNSWFELQFSKDE- 215

Query: 232 ANQISPPPPPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVSGRHSSVGEKRTGGF 291
           +N ++  PP L    P                           R+SGRHSS GE+ +   
Sbjct: 216 SNSVNNNPPGLTRFIP-------------------------VSRISGRHSSAGERAS--- 275

Query: 292 WQNLME-RTGESSSRDPALFRRSLDSS---SAAPRKRTETVAVGCFDR-PRKDGLLLPDE 351
              L E RT  S   +P  FRRSLDSS   + A  +++E+VAV C DR  RKDGLLL   
Sbjct: 276 --RLNEIRTSSSYKSNPMSFRRSLDSSLKVNDAGEEKSESVAVNCLDRLQRKDGLLL--- 335

Query: 352 KPNLQNQNQNQNRFEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMIISESRRYSAA 401
              + N+   + RFEHRII+S     G    QRWS+V+ SDLLYLRSEMI+S+ R+ +AA
Sbjct: 336 ---IPNRESFEGRFEHRIIIS----GGNRDDQRWSEVRPSDLLYLRSEMILSDCRKLAAA 354

BLAST of MS002197 vs. ExPASy Swiss-Prot
Match: Q5XF85 (E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 SV=2)

HSP 1 Score: 161.8 bits (408), Expect = 1.7e-38
Identity = 122/369 (33.06%), Postives = 180/369 (48.78%), Query Frame = 0

Query: 36  FLYRFQALEGAVLVAPPPPFASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYA 95
           F +    L        PPPF +        + A F PS+AVV GVL   F++TF+LL+YA
Sbjct: 5   FFFFLPLLHSYASAQTPPPFRNG------DLVANFEPSLAVVTGVLAIMFALTFVLLVYA 64

Query: 96  KHC----KRGNVVVVGYSRRPNSMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQK 155
           K C    + G+     + RR          + S+ + SG+D+  IESLP+FRF +L G K
Sbjct: 65  KCCHIDLRSGSGDRRRHDRRLRQGIFFNRSTASSDRFSGLDKTAIESLPLFRFSALKGSK 124

Query: 156 EGLECAVCLNRFEPTEVLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVED 215
           +GL+C+VCL++FE  E+LRLLPKC+HAFH+ C+D WL+ H+TCPLCR RV  E     ED
Sbjct: 125 QGLDCSVCLSKFESVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRDRVSME-----ED 184

Query: 216 VNILVHNQAAASAGANQISPPPPPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVS 275
            ++L +  +      ++I              R+  S ++ +  E         E  R+ 
Sbjct: 185 SSVLTNGNSFRFLNQSEI--------------REDSSLELYIERE--------EEEERIH 244

Query: 276 GRHSSVGEKRTGGFWQNLMERTGESSSRDPALFRRSLDSSSAAPRKRTETVAVGCFDRPR 335
            R    G  R            GES       FR+ L   +                   
Sbjct: 245 -REELSGSSRFS---------IGES-------FRKILKLGN------------------- 298

Query: 336 KDGLLLPDEKPNLQNQNQNQNRFEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMII 395
           K+  LL DE  N +++ +  ++F HRI+VS         + RWS+V +SDL++L SEM+ 
Sbjct: 305 KEKTLL-DEHVNDKDEKKLMHKFNHRIVVS-----DVVFKNRWSNVSSSDLMFLNSEMVN 298

Query: 396 S-ESRRYSA 400
           S  S R+S+
Sbjct: 365 SISSERFSS 298

BLAST of MS002197 vs. ExPASy Swiss-Prot
Match: Q9SL78 (Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 5.1e-35
Identity = 113/347 (32.56%), Postives = 166/347 (47.84%), Query Frame = 0

Query: 57  SAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAK---HCKRGNVVVVGYSRRPN 116
           S PPP        F+PS+A++ GV +  F++TF+LL+YAK   +  R      G   R +
Sbjct: 24  SPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFHNDLRSETDSDGERIRHD 83

Query: 117 SMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTEVLRLLP 176
            +  G     S  + SG+D+  IESLP FRF +L G K+GLEC+VCL++FE  E+LRLLP
Sbjct: 84  RLWQGLFNRSS--RFSGLDKKAIESLPFFRFSALKGLKQGLECSVCLSKFEDVEILRLLP 143

Query: 177 KCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNILVHNQAAASAGANQISPPP 236
           KC+HAFH+ C+D WL+ H+TCPLCR RV+ ED     D+++L ++  +            
Sbjct: 144 KCRHAFHIGCIDQWLEQHATCPLCRNRVNIED-----DLSVLGNSSTSLR---------- 203

Query: 237 PPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVSGRHSSVGEKRTGGFWQNLMERT 296
                             +LN    R    + E                   +    E T
Sbjct: 204 ------------------ILNQSETREEDSRLE------------------IYIEREEGT 263

Query: 297 GESSSRDPALFRRSLDSSSAAPRKRTETVAVGCFDRPRKDGLLLPDEKPNLQNQNQNQNR 356
            + SSR  + FR+ L  S    R+  E +                DEK       +  ++
Sbjct: 264 NDGSSRFSS-FRKILKKSLLLEREGNENI----------------DEK-------KLMHK 288

Query: 357 FEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMIIS-ESRRYSA 400
           F HRI+VS         + RWS++ +SDL +L SEM+ S  S R+S+
Sbjct: 324 FNHRIVVS-----DAVFKNRWSNITSSDLTFLTSEMLNSVSSDRFSS 288

BLAST of MS002197 vs. ExPASy Swiss-Prot
Match: Q8W571 (RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3)

HSP 1 Score: 112.8 bits (281), Expect = 9.0e-24
Identity = 65/168 (38.69%), Postives = 91/168 (54.17%), Query Frame = 0

Query: 38  YRFQALEGAVLV---APPPPFASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLY 97
           +R+  L  A+++   A    F+ +PP     +     PS   V  VL T F +T LL +Y
Sbjct: 11  HRWIILHVAIIIQSKANAQSFSPSPP----DLQTGHTPSKTTVFAVLVTLFFLTGLLSVY 70

Query: 98  AKHCKRGNV-VVVGYSRRPNSMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEG 157
            +HC R N      Y R   + GS         +  G+D AV+ES P+F + S+   K G
Sbjct: 71  IRHCARSNPDSSTRYFRNRANDGSS--------RRGGLDNAVVESFPVFAYSSVKESKIG 130

Query: 158 ---LECAVCLNRFEPTEVLRLLPKCKHAFHVECVDTWLDAHSTCPLCR 199
              LECA+CLN  E  E +RLLP C H FH++C+DTWL +H+TCP+CR
Sbjct: 131 SKDLECAICLNELEDHETVRLLPICNHLFHIDCIDTWLYSHATCPVCR 166

BLAST of MS002197 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 4.5e-23
Identity = 55/169 (32.54%), Postives = 88/169 (52.07%), Query Frame = 0

Query: 47  VLVAPPPPFASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKR------ 106
           V+  PPP F      E+    + F P +  ++G+L + F +     L +K+C R      
Sbjct: 35  VIFPPPPSFYL--DDEDDSSSSDFSPLLIALIGILASAFILVSYYTLISKYCHRRRHNSS 94

Query: 107 -----------GNVVVVGYSRRPNSMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSG 166
                       +    G +   N+  + PN +       G+D+++I+S+ ++++  + G
Sbjct: 95  STSAAAINRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDG 154

Query: 167 QKEGLECAVCLNRFEPTEVLRLLPKCKHAFHVECVDTWLDAHSTCPLCR 199
             E  +C+VCL+ F+  E LRLLPKC HAFHV C+DTWL +HS CPLCR
Sbjct: 155 FVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCR 201

BLAST of MS002197 vs. ExPASy TrEMBL
Match: A0A5D3DZ82 (RING-H2 finger protein ATL43 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001970 PE=4 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.8e-147
Identity = 311/427 (72.83%), Postives = 323/427 (75.64%), Query Frame = 0

Query: 1   MGALPNPFSFFFFLLLLFFSMAAADQAPDLRF----------PRPFLYRFQALEGAVLVA 60
           MG LPN FS  F L  LF +++ A    DLR              FL    +   +    
Sbjct: 1   MGTLPNSFS-LFSLFFLFIAVSPAAPTTDLRSLNLILTHHLKETVFLASSSSSSSSSSSP 60

Query: 61  PPPPF-ASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN-VVVVGY 120
           PPPPF  SAPPPEEKPVY+PFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN  VVVGY
Sbjct: 61  PPPPFIPSAPPPEEKPVYSPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNGAVVVGY 120

Query: 121 SRRPN-SMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTE 180
           S RPN +MG    PSFS RKNSGIDQ VIESLPIFRFGSLSGQKEGLECAVCLNRFEPTE
Sbjct: 121 SMRPNTTMGV---PSFSTRKNSGIDQTVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTE 180

Query: 181 VLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNILVHNQAAASAGAN 240
           VLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNI +HNQ        
Sbjct: 181 VLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNIFLHNQ-------- 240

Query: 241 QISPPPPPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVSGRHSSVGEKRTGGFWQ 300
                 PP PPPPPPPR+S SKDVVLNLE GRRS GKA SGRVSGRHSSVGEK       
Sbjct: 241 ------PPPPPPPPPPRESNSKDVVLNLEQGRRS-GKAGSGRVSGRHSSVGEK------- 300

Query: 301 NLMERTGESSSRDPALFRRSLDSSSAAPRKRTETVAVGCFDRPRKDGLLLPDEKPNLQNQ 360
               RTGESS RDPAL RRSLDS      KRTETV+VGCFDR RKDGLLLP+EK N QNQ
Sbjct: 301 ----RTGESSYRDPALLRRSLDS------KRTETVSVGCFDRHRKDGLLLPEEKSN-QNQ 360

Query: 361 NQNQNRFEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMIISESRRYSAASLPPELK 415
           NQNQNR EHRIIVSPR    E    RWSDVQ SDLLYLRSEMIIS+SRRYS ASLP ELK
Sbjct: 361 NQNQNRLEHRIIVSPRVPVVE----RWSDVQGSDLLYLRSEMIISDSRRYSVASLPVELK 386

BLAST of MS002197 vs. ExPASy TrEMBL
Match: A0A6J1I903 (RING-H2 finger protein ATL43 OS=Cucurbita maxima OX=3661 GN=LOC111472589 PE=4 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 3.0e-147
Identity = 301/425 (70.82%), Postives = 324/425 (76.24%), Query Frame = 0

Query: 1   MGALPNPFSFFFFLLLLFFSMAAA------DQAPDLRFPRPFLYRFQALEGAVLVA---- 60
           MG LP+ F F    L    S AAA      D +  L  P P LYRF+A++  V  A    
Sbjct: 1   MGTLPDSFLFSLIFLFTVVSPAAAAAALPGDPSLHLLLPHPILYRFKAIQETVFDASPSP 60

Query: 61  PPPPFASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNVVVVGYSR 120
           PPPP  S PPPE+KPVY+ FRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN VVVGYSR
Sbjct: 61  PPPPLPSPPPPEDKPVYSTFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNAVVVGYSR 120

Query: 121 RPNS-MGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTEVL 180
            PNS MG+   PSF+ RKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCL++FE TE+L
Sbjct: 121 YPNSVMGA---PSFTTRKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLDKFEQTEIL 180

Query: 181 RLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNILVHNQAAASAGANQI 240
           RLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLL+EDVNI ++N           
Sbjct: 181 RLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLIEDVNIFLYN----------- 240

Query: 241 SPPPPPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVSGRHSSVGEKRTGGFWQNL 300
              P P PPP PPPRDS SKDVVLNLE GR+S GKA SGRVSGRHSSVGEK         
Sbjct: 241 ---PAPAPPPLPPPRDSNSKDVVLNLEQGRKS-GKAGSGRVSGRHSSVGEK--------- 300

Query: 301 MERTGESSSRDPALFRRSLDSSSAAPRKRTETVAVGCFDRPRKDGLLLPDEKPNLQNQNQ 360
             RTGESS RDPAL RRSLDS      KR+ETVA+GCFDR RKDGLLLPDEK    NQNQ
Sbjct: 301 --RTGESSRRDPALLRRSLDS------KRSETVAIGCFDRHRKDGLLLPDEK---TNQNQ 360

Query: 361 NQNRFEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMIISESRRYSAASLPPELKRQ 415
           NQ+RFEHRIIVSPR       +QRWSDV ASDLLYLRSEMIIS+SRR+S ASLP ELKRQ
Sbjct: 361 NQSRFEHRIIVSPRAPVVVQGQQRWSDVHASDLLYLRSEMIISDSRRFSVASLPSELKRQ 387

BLAST of MS002197 vs. ExPASy TrEMBL
Match: A0A6J1EU36 (RING-H2 finger protein ATL43 OS=Cucurbita moschata OX=3662 GN=LOC111437573 PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 3.9e-147
Identity = 298/422 (70.62%), Postives = 322/422 (76.30%), Query Frame = 0

Query: 1   MGALPNPFSFFFFLLLLFFSMAAA----DQAPDLRFPRPFLYRFQALEGAVLVA----PP 60
           MG LP+ F F    L    S AAA    D + +L  P P LYRF+A++  V  A    PP
Sbjct: 1   MGTLPDSFLFSLIFLFTVVSPAAAALPGDPSLNLLLPHPILYRFKAIQETVFDASPSPPP 60

Query: 61  PPFASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNVVVVGYSRRP 120
           PP  S PPPE+KPVY+PFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN VVVGYSR P
Sbjct: 61  PPLPSPPPPEDKPVYSPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNAVVVGYSRYP 120

Query: 121 NSMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTEVLRLL 180
           NS+     PSF+ RKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCL++FE TE+LRLL
Sbjct: 121 NSVIGA--PSFTTRKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLDKFEQTEILRLL 180

Query: 181 PKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNILVHNQAAASAGANQISPP 240
           PKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLL+EDVNI ++N              
Sbjct: 181 PKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLIEDVNIFLYN-------------- 240

Query: 241 PPPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVSGRHSSVGEKRTGGFWQNLMER 300
           P P PPP PPPRDS SKDVVLNLE GR+S GKA SGRVSGRHSSVGEK           R
Sbjct: 241 PAPAPPPLPPPRDSNSKDVVLNLEQGRKS-GKAGSGRVSGRHSSVGEK-----------R 300

Query: 301 TGESSSRDPALFRRSLDSSSAAPRKRTETVAVGCFDRPRKDGLLLPDEKPNLQNQNQNQN 360
           TGESS RDPAL RRSLDS      K +ETVA+GCFDR RKDGLLLPDEK      NQNQ+
Sbjct: 301 TGESSRRDPALLRRSLDS------KSSETVAIGCFDRHRKDGLLLPDEK-----TNQNQS 360

Query: 361 RFEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMIISESRRYSAASLPPELKRQRRM 415
           RFEHRIIVSPR       +QRWSDV ASDLLYLRSEMIIS+SRR+S ASLPPELKRQRRM
Sbjct: 361 RFEHRIIVSPRAPVVVQGQQRWSDVHASDLLYLRSEMIISDSRRFSVASLPPELKRQRRM 383

BLAST of MS002197 vs. ExPASy TrEMBL
Match: A0A5A7UX38 (RING-H2 finger protein ATL43 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G005050 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 5.7e-146
Identity = 311/428 (72.66%), Postives = 323/428 (75.47%), Query Frame = 0

Query: 1   MGALPNPFSFFFFLLLLFFSMAAADQAPDLRFPRPFLYRFQALEGAVLVA---------- 60
           MG LPN FS  F L  LF +++ A    DLR     L     L+  V +A          
Sbjct: 1   MGTLPNSFS-LFSLFFLFIAVSPAAPTTDLRSLNLILTHH--LKETVFLASSSSSSSSSS 60

Query: 61  -PPPPF-ASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN-VVVVG 120
            PPPPF  SAPPPEEKPVY+PFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN  VVVG
Sbjct: 61  PPPPPFIPSAPPPEEKPVYSPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNGAVVVG 120

Query: 121 YSRRPN-SMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEPT 180
           YS RPN +MG    PSFS RKNSGIDQ VIESLPIFRFGSLSGQKEGLECAVCLNRFEPT
Sbjct: 121 YSMRPNTTMGV---PSFSTRKNSGIDQTVIESLPIFRFGSLSGQKEGLECAVCLNRFEPT 180

Query: 181 EVLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNILVHNQAAASAGA 240
           EVLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNI +HNQ       
Sbjct: 181 EVLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNIFLHNQ------- 240

Query: 241 NQISPPPPPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVSGRHSSVGEKRTGGFW 300
                  PP PPPPPPPR+S SKDVVLNLE GRRS GKA SGRVSGRHSSVGEK      
Sbjct: 241 -------PPPPPPPPPPRESNSKDVVLNLEQGRRS-GKAGSGRVSGRHSSVGEK------ 300

Query: 301 QNLMERTGESSSRDPALFRRSLDSSSAAPRKRTETVAVGCFDRPRKDGLLLPDEKPNLQN 360
                RTGESS RDPAL RRSLDS      KRTETV+VGCFDR RKDGLLLP+EK    N
Sbjct: 301 -----RTGESSYRDPALLRRSLDS------KRTETVSVGCFDRHRKDGLLLPEEK---SN 360

Query: 361 QNQNQNRFEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMIISESRRYSAASLPPEL 415
           QNQNQNR EHRIIVSPR    E    RWSDVQ SDLLYLRSEMIIS+SRRYS ASLP EL
Sbjct: 361 QNQNQNRLEHRIIVSPRVPVVE----RWSDVQGSDLLYLRSEMIISDSRRYSVASLPVEL 383

BLAST of MS002197 vs. ExPASy TrEMBL
Match: A0A0A0M016 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G433100 PE=4 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.6e-145
Identity = 307/424 (72.41%), Postives = 320/424 (75.47%), Query Frame = 0

Query: 1   MGALPNPFSFFFFLLLLFFSMAAADQAPDLRFPRPF-LYRFQALEGAVLVA------PPP 60
           MG LPN FS F     LFF   A   A    + RP  L     L+  V +A      PPP
Sbjct: 1   MGTLPNSFSLF----SLFFLFTAVSPAAPTTYHRPLNLILTHPLKETVFLASSSSSPPPP 60

Query: 61  P--FASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN-VVVVGYSR 120
           P    SAPPPEEKPVY+PFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGN  VVVGYS 
Sbjct: 61  PLFIPSAPPPEEKPVYSPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKRGNAAVVVGYSM 120

Query: 121 RPNSMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTEVLR 180
           RPN+M     PSFS RKNSGIDQ VIESLPIFRFGSLSGQKEGLECAVCLNRFEPTEVLR
Sbjct: 121 RPNTMMG--VPSFSTRKNSGIDQTVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTEVLR 180

Query: 181 LLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNILVHNQAAASAGANQIS 240
           LLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNI +H+Q           
Sbjct: 181 LLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNIFLHDQ----------- 240

Query: 241 PPPPPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVSGRHSSVGEKRTGGFWQNLM 300
            PPPP  PPPPPPR+S SKDVVLNLE GRRS GKA SGRVSGRHSSVGEK          
Sbjct: 241 -PPPPPQPPPPPPRESNSKDVVLNLEQGRRS-GKAGSGRVSGRHSSVGEK---------- 300

Query: 301 ERTGESSSRDPALFRRSLDSSSAAPRKRTETVAVGCFDRPRKDGLLLPDEKPNLQNQNQN 360
            RTGESS RDPAL RRSLDS      KRTETV+VGCFDR RKDGLLLP+EK    NQNQN
Sbjct: 301 -RTGESSYRDPALLRRSLDS------KRTETVSVGCFDRHRKDGLLLPEEK---TNQNQN 360

Query: 361 QNRFEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMIISESRRYSAASLPPELKRQR 415
           QNR EHRIIVSP+    E    RWSDVQ SDLLYLRSEMIIS+SRR+S ASLP ELKRQR
Sbjct: 361 QNRLEHRIIVSPKVPVVE----RWSDVQGSDLLYLRSEMIISDSRRFSVASLPVELKRQR 381

BLAST of MS002197 vs. TAIR 10
Match: AT5G05810.1 (RING/U-box superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 8.0e-76
Identity = 178/340 (52.35%), Postives = 213/340 (62.65%), Query Frame = 0

Query: 72  PSMAVVVGVLTTTFSITFLLLLYAKHCKRGN-VVVVGYSRR--PNSMGSG---PNPSFSA 131
           P +AVV+ VLT  FS+TFLLLLY KHCKR N  V V + +R      G G          
Sbjct: 2   PGIAVVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQRFAITRYGGGYYNGGGVVGG 61

Query: 132 RKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTEVLRLLPKCKHAFHVECVD 191
           RKNSGID++VIESLP+FRFG+LSG K+GLECAVCL RFEPTEVLRLLPKCKHAFHVECVD
Sbjct: 62  RKNSGIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHVECVD 121

Query: 192 TWLDAHSTCPLCRYRVDPEDVLLVEDVNILVHNQAAASAGANQISPPPPPLPPPPPPPRD 251
           TWLDAHSTCPLCRYRVDPED+LL+ D N     Q +    +N ++  PP L    P    
Sbjct: 122 TWLDAHSTCPLCRYRVDPEDILLIGDCNSWFELQFSKDE-SNSVNNNPPGLTRFIP---- 181

Query: 252 SKSKDVVLNLEPGRRSGGKAESGRVSGRHSSVGEKRTGGFWQNLME-RTGESSSRDPALF 311
                                  R+SGRHSS GE+ +      L E RT  S   +P  F
Sbjct: 182 ---------------------VSRISGRHSSAGERAS-----RLNEIRTSSSYKSNPMSF 241

Query: 312 RRSLDSS---SAAPRKRTETVAVGCFDR-PRKDGLLLPDEKPNLQNQNQNQNRFEHRIIV 371
           RRSLDSS   + A  +++E+VAV C DR  RKDGLLL      + N+   + RFEHRII+
Sbjct: 242 RRSLDSSLKVNDAGEEKSESVAVNCLDRLQRKDGLLL------IPNRESFEGRFEHRIII 300

Query: 372 SPRGRAGEAQRQRWSDVQASDLLYLRSEMIISESRRYSAA 401
           S     G    QRWS+V+ SDLLYLRSEMI+S+ R+ +AA
Sbjct: 302 S----GGNRDDQRWSEVRPSDLLYLRSEMILSDCRKLAAA 300

BLAST of MS002197 vs. TAIR 10
Match: AT4G28890.1 (RING/U-box superfamily protein )

HSP 1 Score: 161.8 bits (408), Expect = 1.2e-39
Identity = 122/369 (33.06%), Postives = 180/369 (48.78%), Query Frame = 0

Query: 36  FLYRFQALEGAVLVAPPPPFASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYA 95
           F +    L        PPPF +        + A F PS+AVV GVL   F++TF+LL+YA
Sbjct: 5   FFFFLPLLHSYASAQTPPPFRNG------DLVANFEPSLAVVTGVLAIMFALTFVLLVYA 64

Query: 96  KHC----KRGNVVVVGYSRRPNSMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQK 155
           K C    + G+     + RR          + S+ + SG+D+  IESLP+FRF +L G K
Sbjct: 65  KCCHIDLRSGSGDRRRHDRRLRQGIFFNRSTASSDRFSGLDKTAIESLPLFRFSALKGSK 124

Query: 156 EGLECAVCLNRFEPTEVLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVED 215
           +GL+C+VCL++FE  E+LRLLPKC+HAFH+ C+D WL+ H+TCPLCR RV  E     ED
Sbjct: 125 QGLDCSVCLSKFESVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRDRVSME-----ED 184

Query: 216 VNILVHNQAAASAGANQISPPPPPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVS 275
            ++L +  +      ++I              R+  S ++ +  E         E  R+ 
Sbjct: 185 SSVLTNGNSFRFLNQSEI--------------REDSSLELYIERE--------EEEERIH 244

Query: 276 GRHSSVGEKRTGGFWQNLMERTGESSSRDPALFRRSLDSSSAAPRKRTETVAVGCFDRPR 335
            R    G  R            GES       FR+ L   +                   
Sbjct: 245 -REELSGSSRFS---------IGES-------FRKILKLGN------------------- 298

Query: 336 KDGLLLPDEKPNLQNQNQNQNRFEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMII 395
           K+  LL DE  N +++ +  ++F HRI+VS         + RWS+V +SDL++L SEM+ 
Sbjct: 305 KEKTLL-DEHVNDKDEKKLMHKFNHRIVVS-----DVVFKNRWSNVSSSDLMFLNSEMVN 298

Query: 396 S-ESRRYSA 400
           S  S R+S+
Sbjct: 365 SISSERFSS 298

BLAST of MS002197 vs. TAIR 10
Match: AT2G20030.1 (RING/U-box superfamily protein )

HSP 1 Score: 150.2 bits (378), Expect = 3.6e-36
Identity = 113/347 (32.56%), Postives = 166/347 (47.84%), Query Frame = 0

Query: 57  SAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAK---HCKRGNVVVVGYSRRPN 116
           S PPP        F+PS+A++ GV +  F++TF+LL+YAK   +  R      G   R +
Sbjct: 24  SPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFHNDLRSETDSDGERIRHD 83

Query: 117 SMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEGLECAVCLNRFEPTEVLRLLP 176
            +  G     S  + SG+D+  IESLP FRF +L G K+GLEC+VCL++FE  E+LRLLP
Sbjct: 84  RLWQGLFNRSS--RFSGLDKKAIESLPFFRFSALKGLKQGLECSVCLSKFEDVEILRLLP 143

Query: 177 KCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDVLLVEDVNILVHNQAAASAGANQISPPP 236
           KC+HAFH+ C+D WL+ H+TCPLCR RV+ ED     D+++L ++  +            
Sbjct: 144 KCRHAFHIGCIDQWLEQHATCPLCRNRVNIED-----DLSVLGNSSTSLR---------- 203

Query: 237 PPLPPPPPPPRDSKSKDVVLNLEPGRRSGGKAESGRVSGRHSSVGEKRTGGFWQNLMERT 296
                             +LN    R    + E                   +    E T
Sbjct: 204 ------------------ILNQSETREEDSRLE------------------IYIEREEGT 263

Query: 297 GESSSRDPALFRRSLDSSSAAPRKRTETVAVGCFDRPRKDGLLLPDEKPNLQNQNQNQNR 356
            + SSR  + FR+ L  S    R+  E +                DEK       +  ++
Sbjct: 264 NDGSSRFSS-FRKILKKSLLLEREGNENI----------------DEK-------KLMHK 288

Query: 357 FEHRIIVSPRGRAGEAQRQRWSDVQASDLLYLRSEMIIS-ESRRYSA 400
           F HRI+VS         + RWS++ +SDL +L SEM+ S  S R+S+
Sbjct: 324 FNHRIVVS-----DAVFKNRWSNITSSDLTFLTSEMLNSVSSDRFSS 288

BLAST of MS002197 vs. TAIR 10
Match: AT4G40070.1 (RING/U-box superfamily protein )

HSP 1 Score: 112.8 bits (281), Expect = 6.4e-25
Identity = 65/168 (38.69%), Postives = 91/168 (54.17%), Query Frame = 0

Query: 38  YRFQALEGAVLV---APPPPFASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLY 97
           +R+  L  A+++   A    F+ +PP     +     PS   V  VL T F +T LL +Y
Sbjct: 11  HRWIILHVAIIIQSKANAQSFSPSPP----DLQTGHTPSKTTVFAVLVTLFFLTGLLSVY 70

Query: 98  AKHCKRGNV-VVVGYSRRPNSMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSGQKEG 157
            +HC R N      Y R   + GS         +  G+D AV+ES P+F + S+   K G
Sbjct: 71  IRHCARSNPDSSTRYFRNRANDGSS--------RRGGLDNAVVESFPVFAYSSVKESKIG 130

Query: 158 ---LECAVCLNRFEPTEVLRLLPKCKHAFHVECVDTWLDAHSTCPLCR 199
              LECA+CLN  E  E +RLLP C H FH++C+DTWL +H+TCP+CR
Sbjct: 131 SKDLECAICLNELEDHETVRLLPICNHLFHIDCIDTWLYSHATCPVCR 166

BLAST of MS002197 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 110.5 bits (275), Expect = 3.2e-24
Identity = 55/169 (32.54%), Postives = 88/169 (52.07%), Query Frame = 0

Query: 47  VLVAPPPPFASAPPPEEKPVYAPFRPSMAVVVGVLTTTFSITFLLLLYAKHCKR------ 106
           V+  PPP F      E+    + F P +  ++G+L + F +     L +K+C R      
Sbjct: 35  VIFPPPPSFYL--DDEDDSSSSDFSPLLIALIGILASAFILVSYYTLISKYCHRRRHNSS 94

Query: 107 -----------GNVVVVGYSRRPNSMGSGPNPSFSARKNSGIDQAVIESLPIFRFGSLSG 166
                       +    G +   N+  + PN +       G+D+++I+S+ ++++  + G
Sbjct: 95  STSAAAINRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDG 154

Query: 167 QKEGLECAVCLNRFEPTEVLRLLPKCKHAFHVECVDTWLDAHSTCPLCR 199
             E  +C+VCL+ F+  E LRLLPKC HAFHV C+DTWL +HS CPLCR
Sbjct: 155 FVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCR 201