MS001877 (gene) Bitter gourd (TR) v1

Overview
NameMS001877
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionvinorine synthase-like
Locationscaffold30: 570172 .. 571449 (+)
RNA-Seq ExpressionMS001877
SyntenyMS001877
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCGGCGTCAAGATCATTTCAAGGGACATAATCAAGCCCGCATCTCCAACGCCACCGTCCCTAATTTACCGCGAACTTTCATTGATCGATCAGATGGCGCCTCCCTCTTACACGCCTGCAATTTTCTTCTATCGCGCTCAGAAATCGGAAAATGTTGCTCATCAATCTGTCAAAGTTGCCCAAGTTAGGGCTTCTCTTGCTCGAACCCTAACTCGCTTCAATCACTTGGCCGGAACAATGACTGAGAGATTCTATGTCGATTGCAACGACGTTGGAGTCGAATTTTCTGAAGCCAGAGTCGATTGCGAGATTTCGATTGTTTTGGAGAAACCAGAAATCGGTGTTTTGCATCGATTGCTACCGCTTGTTTTCACCTCTCGAAATGGCAGCGAGTTTCTGCTTTCCGTTCAGTTCAGTAGCTTCAGCTGCGGCGGTGTAGCCGTTGCCGTTTGTCTTTCTCACAAGCTCGCCGATGGGGCTTCCGCCGCAGCATTTGTTAAAGTGTGGGCCGCCGTCGCCAGAGGAGATAAACATTCTGTGTTGGAGCCCAATTTTGATGCTGTGAAACTCTACCCACCGAGAAAAATTCCAGGTTTCAAGCGAGGAATCGAGGCCTCAAAAGAGAAGATTCTGACCAGGAGACTCGTGTTTGGCAAGTGCCAGATAGCCGCGTTGAAAGAACTGGCTACCGGTGCCGGTGGTGGCTCCGTCGTTAAACCTCCGTCGCGCGTGGTGGCTGTTTCAGCCTTCATTTGGCTTCGTTTAATGGAACTGGCTCGAAAGAGAGCAGTGAAAGCAAAGGTGTTCGGAGCGCTGTATCCGGTGGACTTACGGGCGAGGATGGACCCACCATTGCCGGAAAATTCCTTCGGCAACGTCTCCTGGTTCACAATGGCAACATCCCCTGTGGAGGCGAATGAAGATTTGCCACTTCTGGTGGGTAAAGTGAGAAGCGCCATTCAAGAAATCGACTCTGGATTTGTGAAGAAACTAGAAGATTCAGAAAACTTGTTAGATTTGATTAAGCAAGTGGACAAACAAATTTCCAGCGGGGAGATGCAGCTGTGGAGCTTCACGAGTTGGTGTAGATTTCCGGTGTATGAAGCTGATTTTGGATGGGGAAAGCCGAGTTGGGTTTGCAGCCCGAGCAGGCCTTTTCGAAACGCAGTCGTTTTAATGGATGCTTCTGATGGTTATGGAGTTGAGGCTTGGGTGAACTTGAAGGAAGAAGACATGGCCATCTTCCACCATGACCAACAGCTTCTCTCCTTCTGCTCA

mRNA sequence

ATCGGCGTCAAGATCATTTCAAGGGACATAATCAAGCCCGCATCTCCAACGCCACCGTCCCTAATTTACCGCGAACTTTCATTGATCGATCAGATGGCGCCTCCCTCTTACACGCCTGCAATTTTCTTCTATCGCGCTCAGAAATCGGAAAATGTTGCTCATCAATCTGTCAAAGTTGCCCAAGTTAGGGCTTCTCTTGCTCGAACCCTAACTCGCTTCAATCACTTGGCCGGAACAATGACTGAGAGATTCTATGTCGATTGCAACGACGTTGGAGTCGAATTTTCTGAAGCCAGAGTCGATTGCGAGATTTCGATTGTTTTGGAGAAACCAGAAATCGGTGTTTTGCATCGATTGCTACCGCTTGTTTTCACCTCTCGAAATGGCAGCGAGTTTCTGCTTTCCGTTCAGTTCAGTAGCTTCAGCTGCGGCGGTGTAGCCGTTGCCGTTTGTCTTTCTCACAAGCTCGCCGATGGGGCTTCCGCCGCAGCATTTGTTAAAGTGTGGGCCGCCGTCGCCAGAGGAGATAAACATTCTGTGTTGGAGCCCAATTTTGATGCTGTGAAACTCTACCCACCGAGAAAAATTCCAGGTTTCAAGCGAGGAATCGAGGCCTCAAAAGAGAAGATTCTGACCAGGAGACTCGTGTTTGGCAAGTGCCAGATAGCCGCGTTGAAAGAACTGGCTACCGGTGCCGGTGGTGGCTCCGTCGTTAAACCTCCGTCGCGCGTGGTGGCTGTTTCAGCCTTCATTTGGCTTCGTTTAATGGAACTGGCTCGAAAGAGAGCAGTGAAAGCAAAGGTGTTCGGAGCGCTGTATCCGGTGGACTTACGGGCGAGGATGGACCCACCATTGCCGGAAAATTCCTTCGGCAACGTCTCCTGGTTCACAATGGCAACATCCCCTGTGGAGGCGAATGAAGATTTGCCACTTCTGGTGGGTAAAGTGAGAAGCGCCATTCAAGAAATCGACTCTGGATTTGTGAAGAAACTAGAAGATTCAGAAAACTTGTTAGATTTGATTAAGCAAGTGGACAAACAAATTTCCAGCGGGGAGATGCAGCTGTGGAGCTTCACGAGTTGGTGTAGATTTCCGGTGTATGAAGCTGATTTTGGATGGGGAAAGCCGAGTTGGGTTTGCAGCCCGAGCAGGCCTTTTCGAAACGCAGTCGTTTTAATGGATGCTTCTGATGGTTATGGAGTTGAGGCTTGGGTGAACTTGAAGGAAGAAGACATGGCCATCTTCCACCATGACCAACAGCTTCTCTCCTTCTGCTCA

Coding sequence (CDS)

ATCGGCGTCAAGATCATTTCAAGGGACATAATCAAGCCCGCATCTCCAACGCCACCGTCCCTAATTTACCGCGAACTTTCATTGATCGATCAGATGGCGCCTCCCTCTTACACGCCTGCAATTTTCTTCTATCGCGCTCAGAAATCGGAAAATGTTGCTCATCAATCTGTCAAAGTTGCCCAAGTTAGGGCTTCTCTTGCTCGAACCCTAACTCGCTTCAATCACTTGGCCGGAACAATGACTGAGAGATTCTATGTCGATTGCAACGACGTTGGAGTCGAATTTTCTGAAGCCAGAGTCGATTGCGAGATTTCGATTGTTTTGGAGAAACCAGAAATCGGTGTTTTGCATCGATTGCTACCGCTTGTTTTCACCTCTCGAAATGGCAGCGAGTTTCTGCTTTCCGTTCAGTTCAGTAGCTTCAGCTGCGGCGGTGTAGCCGTTGCCGTTTGTCTTTCTCACAAGCTCGCCGATGGGGCTTCCGCCGCAGCATTTGTTAAAGTGTGGGCCGCCGTCGCCAGAGGAGATAAACATTCTGTGTTGGAGCCCAATTTTGATGCTGTGAAACTCTACCCACCGAGAAAAATTCCAGGTTTCAAGCGAGGAATCGAGGCCTCAAAAGAGAAGATTCTGACCAGGAGACTCGTGTTTGGCAAGTGCCAGATAGCCGCGTTGAAAGAACTGGCTACCGGTGCCGGTGGTGGCTCCGTCGTTAAACCTCCGTCGCGCGTGGTGGCTGTTTCAGCCTTCATTTGGCTTCGTTTAATGGAACTGGCTCGAAAGAGAGCAGTGAAAGCAAAGGTGTTCGGAGCGCTGTATCCGGTGGACTTACGGGCGAGGATGGACCCACCATTGCCGGAAAATTCCTTCGGCAACGTCTCCTGGTTCACAATGGCAACATCCCCTGTGGAGGCGAATGAAGATTTGCCACTTCTGGTGGGTAAAGTGAGAAGCGCCATTCAAGAAATCGACTCTGGATTTGTGAAGAAACTAGAAGATTCAGAAAACTTGTTAGATTTGATTAAGCAAGTGGACAAACAAATTTCCAGCGGGGAGATGCAGCTGTGGAGCTTCACGAGTTGGTGTAGATTTCCGGTGTATGAAGCTGATTTTGGATGGGGAAAGCCGAGTTGGGTTTGCAGCCCGAGCAGGCCTTTTCGAAACGCAGTCGTTTTAATGGATGCTTCTGATGGTTATGGAGTTGAGGCTTGGGTGAACTTGAAGGAAGAAGACATGGCCATCTTCCACCATGACCAACAGCTTCTCTCCTTCTGCTCA

Protein sequence

IGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVAQVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLLPLVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSVLEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVVKPPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMATSPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFTSWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQQLLSFCS
Homology
BLAST of MS001877 vs. NCBI nr
Match: XP_022135580.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 844.7 bits (2181), Expect = 3.4e-241
Identity = 424/426 (99.53%), Postives = 424/426 (99.53%), Query Frame = 0

Query: 1   IGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVA 60
           IGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVA
Sbjct: 4   IGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVA 63

Query: 61  QVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLL 120
           QVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLL
Sbjct: 64  QVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLL 123

Query: 121 PLVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSV 180
           PLVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSV
Sbjct: 124 PLVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSV 183

Query: 181 LEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVVKP 240
           LEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELA GAGGGSVVKP
Sbjct: 184 LEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELAAGAGGGSVVKP 243

Query: 241 PSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMAT 300
           PSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMAT
Sbjct: 244 PSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMAT 303

Query: 301 SPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFTS 360
           SPVEANEDLPLL GKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFTS
Sbjct: 304 SPVEANEDLPLLAGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFTS 363

Query: 361 WCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQQ 420
           WCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQQ
Sbjct: 364 WCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQQ 423

Query: 421 LLSFCS 427
           LLSFCS
Sbjct: 424 LLSFCS 429

BLAST of MS001877 vs. NCBI nr
Match: XP_023529997.1 (vinorine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 666.8 bits (1719), Expect = 1.3e-187
Identity = 333/424 (78.54%), Postives = 368/424 (86.79%), Query Frame = 0

Query: 3   VKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVAQV 62
           V+I+S D+IKP+SPTPPSL YRELSLIDQMA PS TPAIFFYR    +N+AH+S+K+ Q+
Sbjct: 6   VEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPSLTPAIFFYRPSNPQNLAHRSLKLRQI 65

Query: 63  RASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLLPL 122
           RASLARTLT F HLAGTM ERFYV CNDVGV+F EA+VDC+IS +L+ PEIGVL RL+P 
Sbjct: 66  RASLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVLRRLMPS 125

Query: 123 VFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSVLE 182
              SRNGSEFLL VQFSSF CGGVAVAVCLSHKLADGAS AAFVK WAA ARG   SVLE
Sbjct: 126 A-ESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASLAAFVKAWAAYARGGNESVLE 185

Query: 183 PNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVVKPPS 242
            NFDAVKL+PPRKIPGFKR I+ SKEKI ++RLVF K  IAALKELATGAGGGSV  PPS
Sbjct: 186 HNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATGAGGGSVANPPS 245

Query: 243 RVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMATSP 302
           RVVAVSAFIWLRLM LAR+  VKAK+FGALYPV+LRAR DPPLPENSFGNVSWFT+AT+P
Sbjct: 246 RVVAVSAFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFGNVSWFTVATTP 305

Query: 303 VEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFTSWC 362
           V  NEDLPLL  KVR+AIQEIDSGFVK LED E LL+L+KQ+DKQ S+GE+ LWSFTS C
Sbjct: 306 VATNEDLPLLAAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTGEIHLWSFTSMC 365

Query: 363 RFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQQLL 422
           RFP+YEADFGWGKP+WVCSPSRPFRN VVLMDASDG GVEAWVNLKEEDMAIF  D++LL
Sbjct: 366 RFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEEDMAIFEKDEELL 425

Query: 423 SFCS 427
           SFCS
Sbjct: 426 SFCS 428

BLAST of MS001877 vs. NCBI nr
Match: XP_022927112.1 (vinorine synthase-like [Cucurbita moschata])

HSP 1 Score: 666.4 bits (1718), Expect = 1.7e-187
Identity = 334/426 (78.40%), Postives = 369/426 (86.62%), Query Frame = 0

Query: 1   IGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVA 60
           I V+I+S D+IKP+SPTPPSL YRELSLIDQMA P+ TPAIFFYR    +N+A +S+KV 
Sbjct: 4   ISVEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPTLTPAIFFYRPSNPQNLADRSLKVR 63

Query: 61  QVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLL 120
           Q+RASLARTLT F HLAGTM ERFYV CNDVGV+F EA+VDC+IS +L+ PEIGVL RL+
Sbjct: 64  QIRASLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVLRRLM 123

Query: 121 PLVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSV 180
           P    SRNGSEFLL VQFSSF CGGVAVAVCLSHKLADGAS AAFVK WAA ARG   SV
Sbjct: 124 PSA-ESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASVAAFVKAWAAYARGGNESV 183

Query: 181 LEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVVKP 240
           LE NFDAVKL+PPRKIPGFKR I+ SKEKI ++RLVF K  IAALKELATGAGGGSVV P
Sbjct: 184 LEHNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATGAGGGSVVNP 243

Query: 241 PSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMAT 300
           PSRVVAVSAFIWLRLM LAR+  VKAK+FGALYPV+LRAR DPPLPENSFGNVSWFT+AT
Sbjct: 244 PSRVVAVSAFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFGNVSWFTVAT 303

Query: 301 SPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFTS 360
           +PV  NEDLPLLV KVR+AIQEIDSGFVK LED E LL+L+KQ+DKQ S+GE+ LWSFTS
Sbjct: 304 TPVAMNEDLPLLVAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTGEIHLWSFTS 363

Query: 361 WCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQQ 420
            CRFP+YEADFGWGKP+WVCSPSRPFRN VVLMDASDG GVEAWVNLKEEDM IF  D++
Sbjct: 364 MCRFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEEDMTIFEKDEE 423

Query: 421 LLSFCS 427
           LLSFCS
Sbjct: 424 LLSFCS 428

BLAST of MS001877 vs. NCBI nr
Match: XP_038878914.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 665.2 bits (1715), Expect = 3.7e-187
Identity = 334/427 (78.22%), Postives = 368/427 (86.18%), Query Frame = 0

Query: 1   IGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVA 60
           I V+IIS DIIKP+S TP    Y +LSLIDQMAPPSY PAIFFY  Q+SEN+AHQS+K  
Sbjct: 4   INVEIISMDIIKPSSSTPD---YCQLSLIDQMAPPSYMPAIFFYPPQESENLAHQSLKAI 63

Query: 61  QVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLL 120
           Q+R SL+RTLTRF  LAGTM ERFYVDCND GV FSE+++DCEIS++L  PEI  LH LL
Sbjct: 64  QIRDSLSRTLTRFYPLAGTMVERFYVDCNDAGVSFSESKLDCEISLLLNNPEIRTLHCLL 123

Query: 121 PLVFTSRNG-SEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHS 180
           PLVFTS +  S+FLL++QF+ FSCGGVAVAVC+SHKLADGASAA FVK WAA ARG  +S
Sbjct: 124 PLVFTSNSADSKFLLAIQFNRFSCGGVAVAVCVSHKLADGASAAGFVKAWAADARGGSNS 183

Query: 181 VLEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVVK 240
           +LEPNFDA KL+P RKIPGFKRGIEASKEKI T+RLVF K  I  LK LA    GGS  +
Sbjct: 184 ILEPNFDAAKLFPCRKIPGFKRGIEASKEKISTKRLVFSKSDIDVLKSLAVNTDGGSAAE 243

Query: 241 PPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMA 300
           PPSRVVAVSAF+WLRLM LAR R VKAKVFGALYPVDLR RMDPPLPENSFGNVSWFT+A
Sbjct: 244 PPSRVVAVSAFLWLRLMALARTRPVKAKVFGALYPVDLRTRMDPPLPENSFGNVSWFTIA 303

Query: 301 TSPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFT 360
           TSPVE NEDLPLLV KVR+AIQEIDSGFVKKLEDSE+LLDL+KQVDKQ+SSGE+ LWSFT
Sbjct: 304 TSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSEHLLDLMKQVDKQLSSGEIYLWSFT 363

Query: 361 SWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQ 420
           SWCRFPVYEADFGWGKP+WVCSPSRPFRNAVVLMD SDG GVEAWVNLKEEDMAIF  D+
Sbjct: 364 SWCRFPVYEADFGWGKPTWVCSPSRPFRNAVVLMDTSDGDGVEAWVNLKEEDMAIFEKDE 423

Query: 421 QLLSFCS 427
           +LLSFCS
Sbjct: 424 ELLSFCS 427

BLAST of MS001877 vs. NCBI nr
Match: KAG7021414.1 (Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 659.4 bits (1700), Expect = 2.0e-185
Identity = 330/426 (77.46%), Postives = 366/426 (85.92%), Query Frame = 0

Query: 1   IGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVA 60
           I V+I+S D+IKP+SPTPPSL YRELSLIDQMA P+ TPAIFFYR    +N+A +S+KV 
Sbjct: 349 ISVEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPTLTPAIFFYRPSNPQNLADRSLKVR 408

Query: 61  QVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLL 120
           Q+RASLARTLT F HLAGTM ERFYV CNDVGV+F EA+VDC+IS +L+ PEIGVL RL+
Sbjct: 409 QIRASLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVLRRLM 468

Query: 121 PLVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSV 180
           P    SRNGSEFLL VQFSSF CGGVAVAVCLSHKLADGAS A FVK WAA ARG   SV
Sbjct: 469 PSA-ESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASLADFVKAWAAYARGGNESV 528

Query: 181 LEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVVKP 240
           LE NFDAVKL+PPRKIPGFKR I+ SKEKI ++RLVF K  IAALKELATGAGGGS+V P
Sbjct: 529 LEHNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATGAGGGSLVNP 588

Query: 241 PSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMAT 300
           PSRVVAVS FIWLRLM LAR+  VKAK+FGALYPV+LRAR DPPLPENSFGNVSWFT+AT
Sbjct: 589 PSRVVAVSTFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFGNVSWFTVAT 648

Query: 301 SPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFTS 360
           +PV  NEDLPLL  KVR+AIQEIDSGFVK LED E LL+L+KQ+DKQ S+GE+ LWSFTS
Sbjct: 649 TPVAMNEDLPLLAAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTGEIHLWSFTS 708

Query: 361 WCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQQ 420
            CRFP+YEADFGWGKP+WVCSPSRPFRN VVLMDASDG GVEAWVNLKEEDM IF  D++
Sbjct: 709 MCRFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEEDMTIFEKDEE 768

Query: 421 LLSFCS 427
           LLSFCS
Sbjct: 769 LLSFCS 773

BLAST of MS001877 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 3.5e-63
Identity = 152/427 (35.60%), Postives = 239/427 (55.97%), Query Frame = 0

Query: 3   VKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVAQV 62
           ++I+S ++I+P+SPTP +L   +LS +DQ+    + P I FY  Q   N+  ++ +   +
Sbjct: 5   MQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNL-DRAQRSENL 64

Query: 63  RASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLLPL 122
           + SL+  LT+F  LAG +     VDCND GV F EARV  ++S  ++   I  L++ LP 
Sbjct: 65  KRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQYLP- 124

Query: 123 VFTSRNGSE-------FLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARG 182
            F    G E         L+V+ S F CGG A+ VC+SHK+AD  S A F+  W A  + 
Sbjct: 125 -FQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATCQ- 184

Query: 183 DKHSVLEPNFD-AVKLYPPRK---IPGFKRGIEASKEKILTRRLVFGKCQIAALKELATG 242
           ++  +++PNFD     +PP +    P F        E I+ +R VF K ++ ALK  A  
Sbjct: 185 EETDIVQPNFDLGSHHFPPMESIPAPEF-----LPDENIVMKRFVFDKEKLEALK--AQL 244

Query: 243 AGGGSVVKPPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFG 302
           A   + VK  SRV  V A IW + +++ R +        A   V+LR+RM+PP P+++ G
Sbjct: 245 ASSATEVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVAAQAVNLRSRMNPPFPQSAMG 304

Query: 303 NVSWFTMATSPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSG 362
           N++  TMA +  E ++D   LVG +++++ +ID   VK+L+     LD          + 
Sbjct: 305 NIA--TMAYAVAEEDKDFSDLVGPLKTSLAKIDDEHVKELQKGVTYLD--------YEAE 364

Query: 363 EMQLWSFTSWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEED 419
             +L+SF+SWCR   Y+ DFGWGKP  VC+ + P +N V LMD  +  G+EAW+++ E++
Sbjct: 365 PQELFSFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGMEAWISMAEDE 410

BLAST of MS001877 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 235.0 bits (598), Expect = 1.6e-60
Identity = 151/415 (36.39%), Postives = 222/415 (53.49%), Query Frame = 0

Query: 6   ISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVAQVRAS 65
           +S ++I P+SPTP SL   ++S +DQ+    + P I FY      N+         ++ S
Sbjct: 8   VSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQTS-QHLKQS 67

Query: 66  LARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEK-PEIGVLHRLLPLVF 125
           L++ LT F  LAG +     VDCND GV F EARV  ++S  ++   E+  L + LP   
Sbjct: 68  LSKVLTHFYPLAGRINVNSSVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLDQYLPSAA 127

Query: 126 TSRN----GSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSV 185
                     +  L+V+ S F CGG A+ V LSHK+AD  S A F+  W A  RG+   V
Sbjct: 128 YPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATCRGETEIV 187

Query: 186 LEPNFD-AVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVVK 245
           L PNFD A + +PP  +           E ++ +R VF K +I AL+  A+ A   S  K
Sbjct: 188 L-PNFDLAARHFPP--VDNTPSPELVPDENVVMKRFVFDKEKIGALRAQASSA---SEEK 247

Query: 246 PPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMA 305
             SRV  V A+IW  ++++ R +      F  +  V+LR+RM+PPLP  + GN++    A
Sbjct: 248 NFSRVQLVVAYIWKHVIDVTRAKYGAKNKFVVVQAVNLRSRMNPPLPHYAMGNIATLLFA 307

Query: 306 TSPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFT 365
               E ++D P L+G +R++++        K ED  N  +L+K +         +L SFT
Sbjct: 308 AVDAEWDKDFPDLIGPLRTSLE--------KTEDDHN-HELLKGMTCLYELEPQELLSFT 367

Query: 366 SWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAI 415
           SWCR   Y+ DFGWGKP   C+ + P RNA +LMD   G GVEAW+ + E++MA+
Sbjct: 368 SWCRLGFYDLDFGWGKPLSACTTTFPKRNAALLMDTRSGDGVEAWLPMAEDEMAM 406

BLAST of MS001877 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.9e-53
Identity = 144/428 (33.64%), Postives = 225/428 (52.57%), Query Frame = 0

Query: 3   VKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVAQ- 62
           V+I+S+  IKP+SPTP SL    LS IDQ      T   FFY    + N +H+S K+   
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFY----TYNPSHESSKIGDL 65

Query: 63  VRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLLP 122
           ++ SL++TL  +   AG + E  Y+DCND GVEF E R+   ++ +L++ +      +LP
Sbjct: 66  LKNSLSKTLVSYYQFAGRLIENDYIDCNDEGVEFVEVRIHGRMNDILKRGKSFATDLVLP 125

Query: 123 LVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSVL 182
               + +  + LL VQ S F CGG+A+    SHK++DG S   F+K WA+          
Sbjct: 126 TRIIALH-EDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWASSTSLSTFHKP 185

Query: 183 EPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVVKPP 242
            P   A  ++PP            S ++ L +R VF    I  LK  A   G    ++ P
Sbjct: 186 TPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIEYG----IQKP 245

Query: 243 SRVVAVSAFI--WLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMA 302
           SRV  V+AF+       +L RK     K +  +  V+LR  +   LP+NS GN+  F   
Sbjct: 246 SRVEVVTAFLCQCAANCDLPRK-----KPYAIISAVNLRPYL--ALPQNSIGNIFSFYFC 305

Query: 303 TSPVEANEDLPLLVGKVRSAIQEIDSGFV--KKLEDSENLLDLIKQVDKQISSGEMQLWS 362
            +    +     L+ K+R+  Q++    +  +KL     + +L K +D Q++   +  + 
Sbjct: 306 INDEGMDNQFSALISKLRNGKQKLLENIISKEKLTYESQMQELTKCLD-QLNISSLDTYF 365

Query: 363 FTSWCRFPVYEADFGWGKPSWVCSPSRP-FRNAVVLMDASDGYGVEAWVNLKEEDMAIFH 422
            +SWCRFPVY+ DFGWGKP  V SP +P  ++ ++LMD+ +G G+EA + ++E+ MA F 
Sbjct: 366 CSSWCRFPVYDIDFGWGKPILV-SPFQPHVKDLILLMDSPEGDGIEALITMEEKKMAAFE 415

Query: 423 HDQQLLSF 425
            +++L SF
Sbjct: 426 KNEELRSF 415

BLAST of MS001877 vs. ExPASy Swiss-Prot
Match: Q8GZU0 (Minovincinine 19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=MAT PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 2.0e-50
Identity = 148/454 (32.60%), Postives = 235/454 (51.76%), Query Frame = 0

Query: 1   IGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVA 60
           +  + +S+ +IKP+SPTP SL +  LS  DQ   P Y  A FFY       +   S    
Sbjct: 7   VETETLSKTLIKPSSPTPQSLSHYNLSYNDQNIYPEYIFAGFFYSNPDGHEI---STIRE 66

Query: 61  QVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLL 120
           Q++ SL++TL  +   AG + +  Y+ CND G+EF + R+ C ++ +L KPE+      L
Sbjct: 67  QLQNSLSKTLVSYYPFAGKVVKNDYIHCNDDGIEFVDVRIHCRMNDIL-KPELRSYASEL 126

Query: 121 PLVFTSRNGSEFLLS-VQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVA--RGDK 180
                S  GSE   + VQ S F CGGVAVA  +SHK+AD A+  +F+K WAA        
Sbjct: 127 IRPNRSTVGSEDSTALVQLSHFDCGGVAVAFGISHKVADAATILSFIKDWAASTCDLSSS 186

Query: 181 HSVLEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSV 240
           H V  P   +  ++P  +      G   +    + +R +F    I  LK  A   G    
Sbjct: 187 HDVSTPVLVSDSIFP--RQDNIICGQFPASPNCVRKRFLFSPEAIERLKSKAIEFG---- 246

Query: 241 VKPPSRVVAVSAFIWLRLMELARKRAVK------AKVFGALYPVDLRARMDPPLPENSFG 300
           ++ P+RV  ++AF+  R   +A K A K      +  F  +  V+LR  ++  LP+NS G
Sbjct: 247 IEKPTRVEVLTAFL-CRCATVAGKSAAKNNNCGQSLPFAVIQAVNLRPLLE--LPKNSVG 306

Query: 301 NVSWFTMATSPVEANEDL-------PLLVGKVRSAIQEIDS------GFVKKLEDSENLL 360
           N+   ++  S ++ N+ +        L++G++R A  ++ +       +V +++D  N L
Sbjct: 307 NL--ISIYFSTIKENDTVNIEQEFTKLVIGELRKAKDKLKNLSQEKLNYVARMQDFANCL 366

Query: 361 DLIKQVD----KQISSGEMQLWSFTSWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMD 420
              K++D      + + ++  + F+SWCRFP Y+ DFG GKP WVC     F+N ++LMD
Sbjct: 367 ---KELDISSFFDMENVDIDAYLFSSWCRFPFYDIDFGLGKPIWVCMFQPHFKNCIILMD 426

Query: 421 --ASDGYGVEAWVNLKEEDMAIFHHDQQLLSFCS 427
               D YG+EA + L++E M  F +++ LLSF S
Sbjct: 427 YPFGDDYGIEALITLEQEKMPAFENNELLLSFAS 442

BLAST of MS001877 vs. ExPASy Swiss-Prot
Match: Q58VT1 (Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 5.8e-50
Identity = 138/441 (31.29%), Postives = 224/441 (50.79%), Query Frame = 0

Query: 3   VKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFY-----RAQKSENVAHQSV 62
           V I  +  IKP+S TP +L + +LS IDQ     Y P  FFY     R + S+N    S 
Sbjct: 10  VSICDKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSH 69

Query: 63  KVAQVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKP-----E 122
               ++ SL++TL  +   AG + +   VDCND+G EF   R+ C +S +L+ P     E
Sbjct: 70  IAHLLQTSLSQTLVSYYPYAGKLKDNATVDCNDMGAEFLSVRIKCSMSEILDHPHASLAE 129

Query: 123 IGVLHRLLPLVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAV 182
             VL + LP       G+  LL VQ S F CGG+A++VC SHK+ DG S   F+  W++V
Sbjct: 130 SIVLPKDLPWANNCEGGN--LLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSV 189

Query: 183 ARGDKHSVL--EPNFDAVKLYPPRKIPG-FKRGIEASKEKILTRRLVFGKCQIAALKELA 242
            R    + L   P F    ++  +K        I +   + + +RL+F   ++ AL+   
Sbjct: 190 TRDHTTTTLVPSPRFVGDSVFSTKKYGSLITPQILSDLNECVQKRLIFPTDKLDALRAKV 249

Query: 243 TGAGGGSVVKPPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENS 302
               G   VK P+R   VSA     L + A K +        ++ +++R  + P LP N+
Sbjct: 250 AEESG---VKNPTRAEVVSAL----LFKCATKASSSMLPSKLVHFLNIRTMIKPRLPRNA 309

Query: 303 FGNVSWFTMATSPVEANEDLPLLVGKVRSAIQEIDSGFVK-KLEDSENLLDLIKQVDK-- 362
            GN+S      +    + +LP LV  +R   +E++  + K ++E +E +L++++ + +  
Sbjct: 310 IGNLSSIFSIEATNMQDMELPTLVRNLR---KEVEVAYKKDQVEQNELILEVVESMREGK 369

Query: 363 ---QISSGEMQLWSFTSWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEA 422
              +   G   +++ ++ C++P Y  DFGWG+P  VC  + P +NA  L D   G GVEA
Sbjct: 370 LPFENMDGYENVYTCSNLCKYPYYTVDFGWGRPERVCLGNGPSKNAFFLKDYKAGQGVEA 429

Query: 423 WVNLKEEDMAIFHHDQQLLSF 425
            V L ++ M+ F  +++LL F
Sbjct: 430 RVMLHKQQMSEFERNEELLEF 438

BLAST of MS001877 vs. ExPASy TrEMBL
Match: A0A6J1C1E9 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111007495 PE=3 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 1.6e-241
Identity = 424/426 (99.53%), Postives = 424/426 (99.53%), Query Frame = 0

Query: 1   IGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVA 60
           IGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVA
Sbjct: 4   IGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVA 63

Query: 61  QVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLL 120
           QVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLL
Sbjct: 64  QVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLL 123

Query: 121 PLVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSV 180
           PLVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSV
Sbjct: 124 PLVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSV 183

Query: 181 LEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVVKP 240
           LEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELA GAGGGSVVKP
Sbjct: 184 LEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELAAGAGGGSVVKP 243

Query: 241 PSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMAT 300
           PSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMAT
Sbjct: 244 PSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMAT 303

Query: 301 SPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFTS 360
           SPVEANEDLPLL GKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFTS
Sbjct: 304 SPVEANEDLPLLAGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFTS 363

Query: 361 WCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQQ 420
           WCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQQ
Sbjct: 364 WCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQQ 423

Query: 421 LLSFCS 427
           LLSFCS
Sbjct: 424 LLSFCS 429

BLAST of MS001877 vs. ExPASy TrEMBL
Match: A0A6J1EG83 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111434048 PE=3 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 8.0e-188
Identity = 334/426 (78.40%), Postives = 369/426 (86.62%), Query Frame = 0

Query: 1   IGVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVA 60
           I V+I+S D+IKP+SPTPPSL YRELSLIDQMA P+ TPAIFFYR    +N+A +S+KV 
Sbjct: 4   ISVEIVSNDVIKPSSPTPPSLTYRELSLIDQMAAPTLTPAIFFYRPSNPQNLADRSLKVR 63

Query: 61  QVRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLL 120
           Q+RASLARTLT F HLAGTM ERFYV CNDVGV+F EA+VDC+IS +L+ PEIGVL RL+
Sbjct: 64  QIRASLARTLTSFKHLAGTMAERFYVHCNDVGVQFLEAKVDCQISDLLDNPEIGVLRRLM 123

Query: 121 PLVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSV 180
           P    SRNGSEFLL VQFSSF CGGVAVAVCLSHKLADGAS AAFVK WAA ARG   SV
Sbjct: 124 PSA-ESRNGSEFLLVVQFSSFICGGVAVAVCLSHKLADGASVAAFVKAWAAYARGGNESV 183

Query: 181 LEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVVKP 240
           LE NFDAVKL+PPRKIPGFKR I+ SKEKI ++RLVF K  IAALKELATGAGGGSVV P
Sbjct: 184 LEHNFDAVKLFPPRKIPGFKREIQPSKEKISSKRLVFTKSHIAALKELATGAGGGSVVNP 243

Query: 241 PSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMAT 300
           PSRVVAVSAFIWLRLM LAR+  VKAK+FGALYPV+LRAR DPPLPENSFGNVSWFT+AT
Sbjct: 244 PSRVVAVSAFIWLRLMALARRSPVKAKLFGALYPVNLRARRDPPLPENSFGNVSWFTVAT 303

Query: 301 SPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFTS 360
           +PV  NEDLPLLV KVR+AIQEIDSGFVK LED E LL+L+KQ+DKQ S+GE+ LWSFTS
Sbjct: 304 TPVAMNEDLPLLVAKVRTAIQEIDSGFVKNLEDPEKLLNLMKQLDKQRSTGEIHLWSFTS 363

Query: 361 WCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQQ 420
            CRFP+YEADFGWGKP+WVCSPSRPFRN VVLMDASDG GVEAWVNLKEEDM IF  D++
Sbjct: 364 MCRFPIYEADFGWGKPTWVCSPSRPFRNVVVLMDASDGDGVEAWVNLKEEDMTIFEKDEE 423

Query: 421 LLSFCS 427
           LLSFCS
Sbjct: 424 LLSFCS 428

BLAST of MS001877 vs. ExPASy TrEMBL
Match: A0A5D3BIG7 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00830 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 1.0e-182
Identity = 331/427 (77.52%), Postives = 366/427 (85.71%), Query Frame = 0

Query: 2   GVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVAQ 61
           GV+II+ DIIKP+S TP    Y +LSLIDQMAPPSY PAIFFY    S+N+ H      Q
Sbjct: 5   GVEIIAMDIIKPSSSTPN---YFQLSLIDQMAPPSYMPAIFFYLPPNSQNIVH------Q 64

Query: 62  VRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLLP 121
           +R SL+RTLTRF  LAGTM ERFYVDCND GVEFSEA+VDCEIS++L+ PEI +LHRLLP
Sbjct: 65  IRDSLSRTLTRFYPLAGTMPERFYVDCNDSGVEFSEAKVDCEISVLLKNPEIRILHRLLP 124

Query: 122 LVFTSRNG-SEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVAR-GDKHS 181
           LVFTS +  S+FLL+VQ + F CGGVAVAVCLSHKLADGASAA FVK WAA AR G  +S
Sbjct: 125 LVFTSHSADSKFLLAVQLTRFICGGVAVAVCLSHKLADGASAAGFVKAWAAEARGGSNNS 184

Query: 182 VLEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVVK 241
           +LEPNFDAVKL+P RKIPGFKRGI ASKEKI T+R VF K  I  LK LA G  GGS+ K
Sbjct: 185 ILEPNFDAVKLFPCRKIPGFKRGIVASKEKISTKRFVFAKSDIDVLKSLAVGTFGGSISK 244

Query: 242 PPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMA 301
           PPSRVVAVSAFIWLRLM LAR R VKAKVFGALYPVDLRARMDPPLP+NSFGNVSWFT+A
Sbjct: 245 PPSRVVAVSAFIWLRLMALARTRPVKAKVFGALYPVDLRARMDPPLPKNSFGNVSWFTIA 304

Query: 302 TSPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFT 361
           TSPVE NEDLPLLV KVR+AIQEIDSGFVKKLEDSE+LL+L+KQVDKQ+SSGE+ LWSFT
Sbjct: 305 TSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSEHLLELMKQVDKQLSSGEVHLWSFT 364

Query: 362 SWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQ 421
           SWCRFPVYEADFGWGKP+WVCSPSRPFRN+VVLMD+SDG GVEAWVNLKEEDMAIF  D+
Sbjct: 365 SWCRFPVYEADFGWGKPTWVCSPSRPFRNSVVLMDSSDGDGVEAWVNLKEEDMAIFEKDE 422

Query: 422 QLLSFCS 427
           +LLSFCS
Sbjct: 425 ELLSFCS 422

BLAST of MS001877 vs. ExPASy TrEMBL
Match: A0A5A7USW8 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G00800 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.3e-182
Identity = 331/427 (77.52%), Postives = 366/427 (85.71%), Query Frame = 0

Query: 2   GVKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVAQ 61
           GV+II+ DIIKP+S TP    Y +LSLIDQMAPPSY PAIFFY    S+N+ H      Q
Sbjct: 5   GVEIIAMDIIKPSSSTPN---YFQLSLIDQMAPPSYMPAIFFYLPPNSQNIVH------Q 64

Query: 62  VRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLLP 121
           +R SL+RTLTRF  LAGTM ERFYVDCND GVEFSEA+VDCEIS++L+ PEI +LHRLLP
Sbjct: 65  IRDSLSRTLTRFYPLAGTMPERFYVDCNDSGVEFSEAKVDCEISVLLKNPEIRILHRLLP 124

Query: 122 LVFTSRNG-SEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGD-KHS 181
           LVFTS +  S+FLL+VQ + F CGGVAVAVCLSHKLADGASAA FVK WAA ARG   +S
Sbjct: 125 LVFTSHSADSKFLLAVQLTRFICGGVAVAVCLSHKLADGASAAGFVKAWAAEARGGCNNS 184

Query: 182 VLEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVVK 241
           +LEPNFDAVKL+P RKIPGFKRGI ASKEKI T+R VF K  I  LK LA G  GGS+ K
Sbjct: 185 ILEPNFDAVKLFPCRKIPGFKRGIVASKEKISTKRFVFAKSDIDVLKSLAVGTFGGSISK 244

Query: 242 PPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMA 301
           PPSRVVAVSAFIWLRLM LAR R VKAKVFGALYPVDLRARMDPPLP+NSFGNVSWFT+A
Sbjct: 245 PPSRVVAVSAFIWLRLMALARTRPVKAKVFGALYPVDLRARMDPPLPKNSFGNVSWFTIA 304

Query: 302 TSPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFT 361
           TSPVE NEDLPLLV KVR+AIQEIDSGFVKKLEDSE+LL+L+KQVDKQ+SSGE+ LWSFT
Sbjct: 305 TSPVEINEDLPLLVAKVRTAIQEIDSGFVKKLEDSEHLLELMKQVDKQLSSGEVHLWSFT 364

Query: 362 SWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQ 421
           SWCRFPVYEADFGWGKP+WVCSPSRPFRN+VVLMD+SDG GVEAWVNLKEEDMAIF  D+
Sbjct: 365 SWCRFPVYEADFGWGKPTWVCSPSRPFRNSVVLMDSSDGDGVEAWVNLKEEDMAIFEKDE 422

Query: 422 QLLSFCS 427
           +LLSFCS
Sbjct: 425 ELLSFCS 422

BLAST of MS001877 vs. ExPASy TrEMBL
Match: A0A1S3CLJ4 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103502350 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.7e-158
Identity = 283/349 (81.09%), Postives = 310/349 (88.83%), Query Frame = 0

Query: 80  MTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLLPLVFTSRNG-SEFLLSVQF 139
           M ERFYVDCND GVEFSEA+VDCEIS++L+ PEI +LHRLLPLVFTS +  S+FLL+VQ 
Sbjct: 1   MPERFYVDCNDSGVEFSEAKVDCEISVLLKNPEIRILHRLLPLVFTSHSADSKFLLAVQL 60

Query: 140 SSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVAR-GDKHSVLEPNFDAVKLYPPRKIP 199
           + F CGGVAVAVCLSHKLADGASAA FVK WAA AR G  +S+LEPNFDAVKL+P RKIP
Sbjct: 61  TRFICGGVAVAVCLSHKLADGASAAGFVKAWAAEARGGSNNSILEPNFDAVKLFPCRKIP 120

Query: 200 GFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVVKPPSRVVAVSAFIWLRLME 259
           GFKRGI ASKEKI T+R VF K  I  LK LA G  GGS+ KPPSRVVAVSAFIWLRLM 
Sbjct: 121 GFKRGIVASKEKISTKRFVFAKSDIDVLKSLAVGTFGGSISKPPSRVVAVSAFIWLRLMA 180

Query: 260 LARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTMATSPVEANEDLPLLVGKVR 319
           LAR R VKAKVFGALYPVDLRARMDPPLP+NSFGNVSWFT+ATSPVE NEDLPLLV KVR
Sbjct: 181 LARTRPVKAKVFGALYPVDLRARMDPPLPKNSFGNVSWFTIATSPVEINEDLPLLVAKVR 240

Query: 320 SAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSGEMQLWSFTSWCRFPVYEADFGWGKPS 379
           +AIQEIDSGFVKKLEDSE+LL+L+KQVDKQ+SSGE+ LWSFTSWCRFPVYEADFGWGKP+
Sbjct: 241 TAIQEIDSGFVKKLEDSEHLLELMKQVDKQLSSGEVHLWSFTSWCRFPVYEADFGWGKPT 300

Query: 380 WVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAIFHHDQQLLSFCS 427
           WVCSPSRPFRN+VVLMD+SDG GVEAWVNLKEEDMAIF  D++LLSFCS
Sbjct: 301 WVCSPSRPFRNSVVLMDSSDGDGVEAWVNLKEEDMAIFEKDEELLSFCS 349

BLAST of MS001877 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 287.0 bits (733), Expect = 2.6e-77
Identity = 161/432 (37.27%), Postives = 248/432 (57.41%), Query Frame = 0

Query: 3   VKIISRDIIKPASPT---PPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKV 62
           + + S++++KP+      PP   +  LS +DQ+APP + P +FFY  + + +   +S   
Sbjct: 5   ITVTSQELVKPSPRNLNHPP--CHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKERS--- 64

Query: 63  AQVRASLARTLTRFNHLAGTM-TERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHR 122
             +++SL+  L  +  LAG +      V CNDVGV F EA+ DC +S +LE P    L++
Sbjct: 65  DHIKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPNELNK 124

Query: 123 LLPLVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKH 182
           L P  F     S+  L+VQ + F CGG+A+ + LSHKL D  S   FV  WAA ARG   
Sbjct: 125 LHPFEF--HEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFARGQTD 184

Query: 183 SVLEPNFDAVKLYPPRKIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSVV 242
            ++ P+FD  K++PP  I         +KE I+TRR VF +  + +L+E  +    G+  
Sbjct: 185 EIITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESLRERFS----GNKK 244

Query: 243 KPPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFTM 302
              +RV  +S FIW R M          K++  ++PV+LR + DP +P+N FGN+  F++
Sbjct: 245 IRATRVEVLSVFIWSRFMASTNHDDKTGKIYTLIHPVNLRRQADPDIPDNMFGNIMRFSV 304

Query: 303 ATSPV---EANEDLPLLVGKVRSAIQEIDSGFVKKL-EDSENLLDLIKQVDKQISSGEMQ 362
               +   E +E+   LV ++R  I++ID+ +VKKL ED+   L+ + +      +GE+ 
Sbjct: 305 TVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDNRGHLEFLNKQASGFVNGEIV 364

Query: 363 LWSFTSWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAI 422
            +SFTS C+FPVYEADFGWGKP WV S    ++N V  +D  +G G+EAW+NL + DM+ 
Sbjct: 365 SFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWINLDQNDMSR 424

Query: 423 FHHDQQLLSFCS 427
           F  D++LL + S
Sbjct: 425 FEADEELLRYVS 425

BLAST of MS001877 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-74
Identity = 168/434 (38.71%), Postives = 235/434 (54.15%), Query Frame = 0

Query: 3   VKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVAQV 62
           V ++SRDIIKP+SPTP  L   +LSL++Q+ P  + P +FFY A  S     Q   +  +
Sbjct: 3   VDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQ---LQML 62

Query: 63  RASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLLPL 122
           + SL+ TLT F  LAG +     +DCND G +F EARV+  +S +L +P    L +L+P 
Sbjct: 63  KKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQLIPT 122

Query: 123 VFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAV-ARGDKHSVL 182
              S      LL  Q S F CG +++ VC+SHKLAD  S   F+K WAA+ +RG   ++ 
Sbjct: 123 SVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGSIKTIG 182

Query: 183 EPNFDAVKLYPPRKI----PGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSV 242
            P FD VK++PP       P      E    + L++R +F    I AL+  A+       
Sbjct: 183 APVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKAS----SFE 242

Query: 243 VKPPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGN-VSWF 302
           V  P+RV AVSA IW   M+  R  +  +K       V LR+R+ PP  +NS GN VS+F
Sbjct: 243 VNQPTRVEAVSALIWKSAMKATRTVSGTSKPSILANSVSLRSRVSPPFTKNSIGNLVSYF 302

Query: 303 TMATSPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLI----KQVDKQISSGE 362
                       L  LV K+R A Q      + KL  + N  ++I    K+    I+SG+
Sbjct: 303 AAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAGDMIASGD 362

Query: 363 MQLWSFTSWCRFPVYEADFGWGKPSWVCSPSRPFRNAVVLMDASDGYGVEAWVNLKEEDM 422
              + F+S CRF +YE DFGWGKP WV  PS   +N V L+D  +  G+EAWVNL E++M
Sbjct: 363 FDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIEAWVNLNEQEM 422

Query: 423 AIFHHDQQLLSFCS 427
            +F  D++LL F S
Sbjct: 423 NLFEQDRELLQFAS 429

BLAST of MS001877 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-62
Identity = 150/432 (34.72%), Postives = 242/432 (56.02%), Query Frame = 0

Query: 3   VKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVA-Q 62
           V+I+SR+I+KP+SPTP       LSL+D ++ P YT A+ FY A     +   + + + +
Sbjct: 5   VEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEETSLK 64

Query: 63  VRASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVDCEISIVLEKPEIGVLHRLLP 122
           ++ SL++TL  F  LAG +   F V+CND G  F EARVD  +S  L+ P    L  L+P
Sbjct: 65  LKKSLSKTLPIFYPLAGRIIGSF-VECNDEGAVFIEARVDHLLSEFLKCPVPESLELLIP 124

Query: 123 LVFTSRNGSEF-LLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSV 182
           +   SR    + +L +Q + FSCGG+ + +C+SHK+ D  S A F++ WA  +RG     
Sbjct: 125 VEAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRG-LGIT 184

Query: 183 LEPNFDAVKLYPP--RKIPG--FKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGS 242
           L P+F A +++P    ++P     R  E  +   +T+R VF   +I  L+  A+     +
Sbjct: 185 LIPSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRAKAS----RN 244

Query: 243 VVKPPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWF 302
           +VK P+RV AV+A  W  + +++R  ++  +       V+LR ++D  L EN+ GN+   
Sbjct: 245 LVKNPTRVEAVTALFWRCVTKVSRLSSLTPRTSVLQILVNLRGKVD-SLCENTIGNMLSL 304

Query: 303 TMATSPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLDLIKQVDKQISSG-EMQL 362
            +  +   A E +  +V ++R A +       +  + S  + +L++++ K    G EM L
Sbjct: 305 MILKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSKSSSRIFELLEEIGKVYGRGNEMDL 364

Query: 363 WSFTSWCRFPVYEADFGWGKPSWVCS-PSRPFRNAVVLMDASDGYGVEAWVNLKEEDMAI 422
           W   SWC+  +Y+ADFGWGKP WV    +  F+N ++L+D  DG G+EAW+ L EE M++
Sbjct: 365 WMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEGIEAWITLTEEQMSL 424

Query: 423 FHHDQQLLSFCS 427
           F  DQ+LL   S
Sbjct: 425 FECDQELLESAS 429

BLAST of MS001877 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 219.9 bits (559), Expect = 3.8e-57
Identity = 149/444 (33.56%), Postives = 239/444 (53.83%), Query Frame = 0

Query: 3   VKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVAQV 62
           V+ IS++IIKP+SPTP +L   +LS+ D + PP YT A  FY      +  H S K   +
Sbjct: 8   VETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDLISQEHTSHK---L 67

Query: 63  RASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARV-DCEISIVLEKPEIGVLHRLLP 122
           + SL+ TLT+F  LAG +T    VDC D G  F +ARV +C ++  L+ P+   L +LLP
Sbjct: 68  KTSLSETLTKFYPLAGRIT-GVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQLLP 127

Query: 123 LVFTSR----NGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARG-- 182
           L           +  LL V+ + F CGG+A+ +C++HK+AD AS + F++ WAA ARG  
Sbjct: 128 LDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATARGEN 187

Query: 183 DKHSVLEPNFDAVKLYPPR----KIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATG 242
           D  ++  P F     YPP     K+P  +   +A K   +T+R VF   + + +++L T 
Sbjct: 188 DAAAMESPVFAGANFYPPANEAFKLPADE---QAGKRSSITKRFVF---EASKVEDLRTK 247

Query: 243 AGGGSVVKPPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFG 302
           A     V  P+RV +V+A IW   +  ++      KV   L   +LR+++   L E+S G
Sbjct: 248 AASEETVDQPTRVESVTALIWKCFVASSKTTTCDHKVLVQL--ANLRSKIPSLLQESSIG 307

Query: 303 NVSWFTMATSPVEANE-DLPLLVGKVRSAIQEIDSGFVKKLEDSEN-------LLDLIKQ 362
           N+ + ++  S     E  +   V  +R   +E+ +  + +   S++       L +L+  
Sbjct: 308 NLMFSSVVLSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIGSKLANLMLT 367

Query: 363 VDKQISSGEMQLWSFTSWCRFPVYEADFGWGKPSWVCSPSRP-FRNAVVLMDASDGYGVE 422
              ++S    + ++ +SWC+ P+YEA FGW  P WV     P   N  +L+D+ DG G+E
Sbjct: 368 NYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLAMLIDSKDGQGIE 427

Query: 423 AWVNLKEEDMAIFHHDQQLLSFCS 427
           A+V L EE+M+ F  + +LL+F +
Sbjct: 428 AFVTLPEENMSSFEQNPELLAFAT 439

BLAST of MS001877 vs. TAIR 10
Match: AT5G47950.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 213.4 bits (542), Expect = 3.6e-55
Identity = 146/433 (33.72%), Postives = 228/433 (52.66%), Query Frame = 0

Query: 3   VKIISRDIIKPASPTPPSLIYRELSLIDQMAPPSYTPAIFFYRAQKSENVAHQSVKVAQV 62
           V+ I ++IIKP++ TP  L   +LS++D + PP Y  A  FY     +++  Q      +
Sbjct: 6   VETIGKEIIKPSATTPTDLSTLQLSIMDILMPPVYAVAFLFY---TKDDLISQEQTSHTL 65

Query: 63  RASLARTLTRFNHLAGTMTERFYVDCNDVGVEFSEARVD-CEISIVLEKPEIGVLHRLLP 122
           + SL+  LT+F+ LAG +     +   D G  F EARVD C++S  L  P+   L +LLP
Sbjct: 66  KTSLSEILTKFHPLAGRV-NGVTIKSTDEGAVFVEARVDNCDLSGFLRSPDTESLKQLLP 125

Query: 123 LVFTSRNGSEFLLSVQFSSFSCGGVAVAVCLSHKLADGASAAAFVKVWAAVARGDKHSVL 182
            V      +  LL V+ + F CGG+A+ +C+SH+LAD AS + F++ WAA ARG+   V 
Sbjct: 126 -VDDEPAPTWPLLLVKATYFRCGGMAIGLCISHRLADAASLSIFLQAWAATARGESDLVA 185

Query: 183 EPNFDAVKLYPPR----KIPGFKRGIEASKEKILTRRLVFGKCQIAALKELATGAGGGSV 242
            P+F + KLYP      KIPG     E      +T+R VF   +I  L+          V
Sbjct: 186 SPDFCSTKLYPAANEAIKIPG-----EVVNRTSVTKRFVFVASKIEELRNKV----ASDV 245

Query: 243 VKPPSRVVAVSAFIWLRLMELARKRAVKAKVFGALYPVDLRARMDPPLPENSFGNVSWFT 302
           V  P+RV +V++ IW + +  A    ++ K      P +LR ++   L EN  GN+  F 
Sbjct: 246 VPRPTRVQSVTSLIW-KCVVTASTDTIREKAL--FQPANLRTKIPSLLSENQIGNL-LFN 305

Query: 303 MATSPVEANEDLPLLVGKVRSAIQEIDSGFVKKLEDSENLLD---LIKQVDKQISSGEMQ 362
                 +A  D+   V +++   +++ SG V+  E S   +      + ++ + +  +  
Sbjct: 306 SLILDGKAGMDIVETVKELQKRAEDL-SGLVQDEEGSSMTIGWRLFGEIINSKFNREDHD 365

Query: 363 LWSFTSWCRFPVYEADFGWGKPSWVCSPSRPFRNAV-VLMDASDGYGVEAWVNLKEEDMA 422
           + S TSWC+ P+Y+A FGWG P WV     P  + V VL+D+ DG G+EAWV L +E+M 
Sbjct: 366 MHSVTSWCKIPLYDACFGWGSPVWVAGSVSPNLDYVTVLIDSKDGQGIEAWVTLPQENML 419

Query: 423 IFHHDQQLLSFCS 427
           +     +LL+F S
Sbjct: 426 LLEQSTELLAFAS 419

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135580.13.4e-24199.53vinorine synthase-like [Momordica charantia][more]
XP_023529997.11.3e-18778.54vinorine synthase-like [Cucurbita pepo subsp. pepo][more]
XP_022927112.11.7e-18778.40vinorine synthase-like [Cucurbita moschata][more]
XP_038878914.13.7e-18778.22stemmadenine O-acetyltransferase-like [Benincasa hispida][more]
KAG7021414.12.0e-18577.46Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A2P1GIW73.5e-6335.60Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
Q70PR71.6e-6036.39Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2[more]
A0A2K8FQU51.9e-5333.64Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT... [more]
Q8GZU02.0e-5032.60Minovincinine 19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=M... [more]
Q58VT15.8e-5031.29Acyltransferase Pun1 OS=Capsicum frutescens OX=4073 GN=PUN1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C1E91.6e-24199.53vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111007495 PE=3 SV=1[more]
A0A6J1EG838.0e-18878.40vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111434048 PE=3 SV=1[more]
A0A5D3BIG71.0e-18277.52Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3... [more]
A0A5A7USW81.3e-18277.52Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2... [more]
A0A1S3CLJ41.7e-15881.09vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103502350 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G24430.12.6e-7737.27HXXXD-type acyl-transferase family protein [more]
AT3G26040.11.2e-7438.71HXXXD-type acyl-transferase family protein [more]
AT1G24420.11.4e-6234.72HXXXD-type acyl-transferase family protein [more]
AT4G15390.13.8e-5733.56HXXXD-type acyl-transferase family protein [more]
AT5G47950.13.6e-5533.72HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..197
e-value: 4.1E-55
score: 188.4
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 207..423
e-value: 5.4E-62
score: 210.7
IPR003480TransferasePFAMPF02458Transferasecoord: 3..421
e-value: 2.1E-81
score: 273.8
NoneNo IPR availablePANTHERPTHR31623:SF25VINORINE SYNTHASE-LIKEcoord: 2..426
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 2..426

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001877.1MS001877.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups