MS001876 (gene) Bitter gourd (TR) v1

Overview
NameMS001876
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionvinorine synthase-like
Locationscaffold30: 565799 .. 567212 (+)
RNA-Seq ExpressionMS001876
SyntenyMS001876
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAGAGAAGATGGCGATCAGAAATATTCAGGTGACCTCCGATGAAATCATCTTGCCGTCTTCTCCGACTCCGCCGCCGCTCAGAAACCTCAAGCTTTCACTGCTCGACCAGATTTCGCCGTCCAATTATACTCCGATCATTTTTTTCTACGCTGCCGTTGAGGACCCCGGGGCCGATCGGTTTTCCGGAAGTCGCCAGTTGAAGAAGTCTTTATCGGAGATTTTGACTCTGTTCTATCCGTTCGCCGGAACTTTATCGACGGAGGGATATTCCGTCGACTGCAACGACAAAGGCGTGGAGTATTCTGAAGCGCGAGTCGATTGCAAGCTATTGGAAGTCGTGGAAAGCCCTGATTTCAAAGTTCTACATCGGTTGCTGCCGTTTGAACCTTACGCTAATCTGGCGGAACCGGAGATCGGAAGAGACGTAATCCTCGCCGTCCGATTAAATCTGTTTGACTGCGGCGGCGTGGCCATTGCTTTGTGTGTTACTCATAAGCTCGTCGACGGACTTTCCGCCACCGTATTTGTCAGAGAGTGGGCGGCCATTAATCGGGGAGATAGCGATCAGGTTCCAAAATTAACTTCTCCGTCAAATACAATTTCGAGCCCTAATTTAGTTTCTTCAGCAGAAAGTGACAAATTAAGAACAATTCCTTTCCAATTTTTTTGAAATGCAGGTTTCGGAACCCTTATTCGACGGCGCAAAGTATTTTCCGCCGAAGGAAATTCCGGGATTGAAGAGAAGCAAGGTCATCCTAAACGACAAGATTGTGACCAAAAGATTCGTGTTCGACAAAGCAAACATAGCCGCACTCCGGCGAGAAGCGGCGGCCGGTTCGCTGGAGAAGCCACCGACGAGAGTAGAGGCCGTTTCGGCATTCTTATGGAAGCGATTCATGGAGATAAATCGAAACTCAGAACAAGAAAACGAAAAGATATACGCCGCAATCCACGCCGTGAACCTGCGCGGGAAGATGGATCCGCCACTGCCGCCGAATTCCTCCGGCAACCTGTGGTGGTTCGCGATCGCCGTGGCAGAGGTTGAAATGAAGAAGCGGCACCAGTACTTGGTGAAGCAGATAAGGAACGCAATAGAAGAAATCGACAGCAATCTGGTGCGGAACTTGCAGCAGGCCGGAGTGCCGCCGCATATGTGGAGCAAGACGGGGAAACACGGGCATCCGGCGGCGGGAGAGGTGGAGTTCTGCAACTTCACGAGCTGGTGCCGGTTTCCGGTGTACGAAGCGGACTTCGGGTGGGGGAAACCGACGTGGGTTTGCAGTCCGAGCCGGCCGTTCAAGAACGTGGTGGTTCTGATGACCACTAGAGACGGCGACGGGATTGAAGCGTGGGTCACGCTCAACGAACAAGATATGGCCTCCTTCCAACAAGATTCTGAGTTAGTTACCTAT

mRNA sequence

TCAGAGAAGATGGCGATCAGAAATATTCAGGTGACCTCCGATGAAATCATCTTGCCGTCTTCTCCGACTCCGCCGCCGCTCAGAAACCTCAAGCTTTCACTGCTCGACCAGATTTCGCCGTCCAATTATACTCCGATCATTTTTTTCTACGCTGCCGTTGAGGACCCCGGGGCCGATCGGTTTTCCGGAAGTCGCCAGTTGAAGAAGTCTTTATCGGAGATTTTGACTCTGTTCTATCCGTTCGCCGGAACTTTATCGACGGAGGGATATTCCGTCGACTGCAACGACAAAGGCGTGGAGTATTCTGAAGCGCGAGTCGATTGCAAGCTATTGGAAGTCGTGGAAAGCCCTGATTTCAAAGTTCTACATCGGTTGCTGCCGTTTGAACCTTACGCTAATCTGGCGGAACCGGAGATCGGAAGAGACGTAATCCTCGCCGTCCGATTAAATCTGTTTGACTGCGGCGGCGTGGCCATTGCTTTGTGTGTTACTCATAAGCTCGTCGACGGACTTTCCGCCACCGTATTTGTCAGAGAGTGGGCGGCCATTAATCGGGGAGATAGCGATCAGGTTTCGGAACCCTTATTCGACGGCGCAAAGTATTTTCCGCCGAAGGAAATTCCGGGATTGAAGAGAAGCAAGGTCATCCTAAACGACAAGATTGTGACCAAAAGATTCGTGTTCGACAAAGCAAACATAGCCGCACTCCGGCGAGAAGCGGCGGCCGGTTCGCTGGAGAAGCCACCGACGAGAGTAGAGGCCGTTTCGGCATTCTTATGGAAGCGATTCATGGAGATAAATCGAAACTCAGAACAAGAAAACGAAAAGATATACGCCGCAATCCACGCCGTGAACCTGCGCGGGAAGATGGATCCGCCACTGCCGCCGAATTCCTCCGGCAACCTGTGGTGGTTCGCGATCGCCGTGGCAGAGGTTGAAATGAAGAAGCGGCACCAGTACTTGGTGAAGCAGATAAGGAACGCAATAGAAGAAATCGACAGCAATCTGGTGCGGAACTTGCAGCAGGCCGGAGTGCCGCCGCATATGTGGAGCAAGACGGGGAAACACGGGCATCCGGCGGCGGGAGAGGTGGAGTTCTGCAACTTCACGAGCTGGTGCCGGTTTCCGGTGTACGAAGCGGACTTCGGGTGGGGGAAACCGACGTGGGTTTGCAGTCCGAGCCGGCCGTTCAAGAACGTGGTGGTTCTGATGACCACTAGAGACGGCGACGGGATTGAAGCGTGGGTCACGCTCAACGAACAAGATATGGCCTCCTTCCAACAAGATTCTGAGTTAGTTACCTAT

Coding sequence (CDS)

TCAGAGAAGATGGCGATCAGAAATATTCAGGTGACCTCCGATGAAATCATCTTGCCGTCTTCTCCGACTCCGCCGCCGCTCAGAAACCTCAAGCTTTCACTGCTCGACCAGATTTCGCCGTCCAATTATACTCCGATCATTTTTTTCTACGCTGCCGTTGAGGACCCCGGGGCCGATCGGTTTTCCGGAAGTCGCCAGTTGAAGAAGTCTTTATCGGAGATTTTGACTCTGTTCTATCCGTTCGCCGGAACTTTATCGACGGAGGGATATTCCGTCGACTGCAACGACAAAGGCGTGGAGTATTCTGAAGCGCGAGTCGATTGCAAGCTATTGGAAGTCGTGGAAAGCCCTGATTTCAAAGTTCTACATCGGTTGCTGCCGTTTGAACCTTACGCTAATCTGGCGGAACCGGAGATCGGAAGAGACGTAATCCTCGCCGTCCGATTAAATCTGTTTGACTGCGGCGGCGTGGCCATTGCTTTGTGTGTTACTCATAAGCTCGTCGACGGACTTTCCGCCACCGTATTTGTCAGAGAGTGGGCGGCCATTAATCGGGGAGATAGCGATCAGGTTTCGGAACCCTTATTCGACGGCGCAAAGTATTTTCCGCCGAAGGAAATTCCGGGATTGAAGAGAAGCAAGGTCATCCTAAACGACAAGATTGTGACCAAAAGATTCGTGTTCGACAAAGCAAACATAGCCGCACTCCGGCGAGAAGCGGCGGCCGGTTCGCTGGAGAAGCCACCGACGAGAGTAGAGGCCGTTTCGGCATTCTTATGGAAGCGATTCATGGAGATAAATCGAAACTCAGAACAAGAAAACGAAAAGATATACGCCGCAATCCACGCCGTGAACCTGCGCGGGAAGATGGATCCGCCACTGCCGCCGAATTCCTCCGGCAACCTGTGGTGGTTCGCGATCGCCGTGGCAGAGGTTGAAATGAAGAAGCGGCACCAGTACTTGGTGAAGCAGATAAGGAACGCAATAGAAGAAATCGACAGCAATCTGGTGCGGAACTTGCAGCAGGCCGGAGTGCCGCCGCATATGTGGAGCAAGACGGGGAAACACGGGCATCCGGCGGCGGGAGAGGTGGAGTTCTGCAACTTCACGAGCTGGTGCCGGTTTCCGGTGTACGAAGCGGACTTCGGGTGGGGGAAACCGACGTGGGTTTGCAGTCCGAGCCGGCCGTTCAAGAACGTGGTGGTTCTGATGACCACTAGAGACGGCGACGGGATTGAAGCGTGGGTCACGCTCAACGAACAAGATATGGCCTCCTTCCAACAAGATTCTGAGTTAGTTACCTAT

Protein sequence

SEKMAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSGSRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLHRLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAINRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAGSLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLWWFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGEVEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDMASFQQDSELVTY
Homology
BLAST of MS001876 vs. NCBI nr
Match: XP_022135273.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 880.6 bits (2274), Expect = 5.7e-252
Identity = 430/432 (99.54%), Postives = 431/432 (99.77%), Query Frame = 0

Query: 4   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSG 63
           MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVED GADRFSG
Sbjct: 1   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDLGADRFSG 60

Query: 64  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH 123
           SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH 120

Query: 124 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI 183
           RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI
Sbjct: 121 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI 180

Query: 184 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG 243
           NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG
Sbjct: 181 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG 240

Query: 244 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 303
           SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 300

Query: 304 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGE 363
           WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDS+LVRNLQQAGVPPHMWSKTGKHGHPAAGE
Sbjct: 301 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSDLVRNLQQAGVPPHMWSKTGKHGHPAAGE 360

Query: 364 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 423
           VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 420

Query: 424 ASFQQDSELVTY 436
           ASFQQDSELVTY
Sbjct: 421 ASFQQDSELVTY 432

BLAST of MS001876 vs. NCBI nr
Match: KAG6587429.1 (CBBY-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 786.9 bits (2031), Expect = 8.6e-224
Identity = 380/432 (87.96%), Postives = 403/432 (93.29%), Query Frame = 0

Query: 4   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSG 63
           MAI  IQV +DEII PSSPTPP  RNLKLSLLDQISPSNYTPIIFFYAAVE+PGADRFSG
Sbjct: 347 MAIEFIQVIADEIISPSSPTPPSRRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 406

Query: 64  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH 123
           SRQLKKSLSEILTLFYPFAGTLS+ GYSVDCND+GVEYSEARV C+L EVVE+PDFKVLH
Sbjct: 407 SRQLKKSLSEILTLFYPFAGTLSSAGYSVDCNDRGVEYSEARVGCELSEVVENPDFKVLH 466

Query: 124 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI 183
           RLLPFEPYAN  EPEIGRDVILAVRLNLFDCGGVAIALC+THKLVDG SATVFVREWAAI
Sbjct: 467 RLLPFEPYANSVEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 526

Query: 184 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG 243
           NRGDSDQVSEPLFD AKYFPPKEIPGLKRSKV+ NDKIVTKRFVFDKANIAALR EAAAG
Sbjct: 527 NRGDSDQVSEPLFDSAKYFPPKEIPGLKRSKVVQNDKIVTKRFVFDKANIAALRHEAAAG 586

Query: 244 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 303
           SLEKPPTRVEAVSAFLWKRFMEIN +SEQ  EK++AA+HAVN+RGKMDPPLPPNSSGNLW
Sbjct: 587 SLEKPPTRVEAVSAFLWKRFMEINPHSEQ--EKVFAAVHAVNIRGKMDPPLPPNSSGNLW 646

Query: 304 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGE 363
           WFAIAVAE E++KRHQYLV QIRNAIEEIDS+LVR LQ AGVP HMWSK GKHGH A GE
Sbjct: 647 WFAIAVAETEIEKRHQYLVNQIRNAIEEIDSDLVRKLQHAGVPSHMWSKMGKHGHAAGGE 706

Query: 364 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 423
           VEFCNFTSWCRFPVYEADFGWGKP+WVCSPSRP+KNVVVLMTTRDGDGIEAWVTL E+DM
Sbjct: 707 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLKEEDM 766

Query: 424 ASFQQDSELVTY 436
             FQ+DSEL+++
Sbjct: 767 ELFQRDSELLSF 776

BLAST of MS001876 vs. NCBI nr
Match: XP_022973602.1 (vinorine synthase-like [Cucurbita maxima])

HSP 1 Score: 785.4 bits (2027), Expect = 2.5e-223
Identity = 379/432 (87.73%), Postives = 402/432 (93.06%), Query Frame = 0

Query: 4   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSG 63
           MAI  IQV +DEII PSSPTPP  RNLK SLLDQISPSNYTPIIFFYAAVE+PGADRFSG
Sbjct: 1   MAIELIQVIADEIISPSSPTPPSRRNLKFSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60

Query: 64  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH 123
           SRQLKKSLSEILTLFYPFAGTLS+ GYSVDCND+GVEYSEARV C+L EVVE+PDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSSAGYSVDCNDRGVEYSEARVGCELSEVVENPDFKVLH 120

Query: 124 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI 183
           RLLPFEPYAN  EPEIGRDVILAVRLNLFDCGGVAIALC+THKLVDG SATVFVREWAAI
Sbjct: 121 RLLPFEPYANSVEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180

Query: 184 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG 243
           NRGDSDQVSEPLFD AKYFPPKEIPGLKRSKV+ NDKIVTKRFVFDKANIAALR EAAAG
Sbjct: 181 NRGDSDQVSEPLFDSAKYFPPKEIPGLKRSKVVQNDKIVTKRFVFDKANIAALRHEAAAG 240

Query: 244 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 303
           SLEKPPTRVEAVSAFLWKRFMEIN +SEQ  EK++AA+HAVN+RGKMDPPLPPNSSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINPHSEQ--EKVFAAVHAVNIRGKMDPPLPPNSSGNLW 300

Query: 304 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGE 363
           WFAIAVAE E++KRHQYLV QIRNAIEEIDS+LVR LQ AGVP HMWSK GKHGH A GE
Sbjct: 301 WFAIAVAETEIEKRHQYLVNQIRNAIEEIDSDLVRKLQHAGVPSHMWSKMGKHGHAAGGE 360

Query: 364 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 423
           VEFCNFTSWCRFPVYEADFGWGKP+WVCSPSRP+KNVVVLMTTRDGDGIEAWVTL E+DM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLKEEDM 420

Query: 424 ASFQQDSELVTY 436
             FQ+DSEL+++
Sbjct: 421 ELFQRDSELLSF 430

BLAST of MS001876 vs. NCBI nr
Match: XP_022933091.1 (vinorine synthase-like [Cucurbita moschata])

HSP 1 Score: 781.9 bits (2018), Expect = 2.8e-222
Identity = 378/432 (87.50%), Postives = 402/432 (93.06%), Query Frame = 0

Query: 4   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSG 63
           MAI  IQV +DEII PSSPTPP  RNLKLSLLDQISPSNYTPIIFFYAAVE+PGAD FSG
Sbjct: 1   MAIEFIQVIADEIISPSSPTPPSRRNLKLSLLDQISPSNYTPIIFFYAAVENPGADWFSG 60

Query: 64  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH 123
           SRQLKKSLSEILTLFYPFAGTLS+ GYSVDCND+GVEYSEARV C+L EVVE+PDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSSAGYSVDCNDRGVEYSEARVGCELSEVVENPDFKVLH 120

Query: 124 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI 183
           RLLPFEPYAN  EPEIGRDVILAVRLNLFDCGGVAIALC+THKLVDG SATVFVREWAAI
Sbjct: 121 RLLPFEPYANSVEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180

Query: 184 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG 243
           NRGDSDQVSEPLFD AKYFPPKEIPGLKRSKV+ NDKIVTKRFVFDKANIAALR EAAAG
Sbjct: 181 NRGDSDQVSEPLFDSAKYFPPKEIPGLKRSKVVQNDKIVTKRFVFDKANIAALRHEAAAG 240

Query: 244 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 303
           SLEKPPTRVEAVSAFLWKRFMEIN +SEQ  EK++AA+HAVN+RGKMDPPLPP+SSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINPHSEQ--EKVFAAVHAVNIRGKMDPPLPPSSSGNLW 300

Query: 304 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGE 363
           WFAIAVAE E++KRHQYLV QIRNAIEEIDS+LVR LQ AGVP HMWSK GKHGH A GE
Sbjct: 301 WFAIAVAETEIEKRHQYLVNQIRNAIEEIDSDLVRKLQHAGVPSHMWSKMGKHGHAAGGE 360

Query: 364 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 423
           VEFCNFTSWCRFPVYEADFGWGKP+WVCSPSRP+KNVVVLMTTRDGDGIEAWVTL E+DM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLKEEDM 420

Query: 424 ASFQQDSELVTY 436
             FQ+DSEL+++
Sbjct: 421 ELFQRDSELLSF 430

BLAST of MS001876 vs. NCBI nr
Match: XP_023531342.1 (vinorine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 778.9 bits (2010), Expect = 2.3e-221
Identity = 376/432 (87.04%), Postives = 400/432 (92.59%), Query Frame = 0

Query: 4   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSG 63
           MAI  IQV +D+II PSSPTPP  RNLKLSLLDQISPSNYTPIIFFYAAVE+PGADRFSG
Sbjct: 1   MAIEFIQVIADQIISPSSPTPPSRRNLKLSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60

Query: 64  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH 123
           SRQLKKSLSEILTLFYPFAGTLS+ GYSVDCND+GVEYSEARV C+L EVVE+PDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSSAGYSVDCNDRGVEYSEARVGCELSEVVENPDFKVLH 120

Query: 124 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI 183
           RLLPFEPYAN  EPEI RDVILAVRLNLFDCGGVAIALC+THKLVDG SATVFVREWAAI
Sbjct: 121 RLLPFEPYANSVEPEIERDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180

Query: 184 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG 243
           NRGDSDQVSEPLFD AKYFPPKEIPGLKRSKV+ NDKIVTKRFVFDKANIAALR EAAAG
Sbjct: 181 NRGDSDQVSEPLFDSAKYFPPKEIPGLKRSKVVQNDKIVTKRFVFDKANIAALRHEAAAG 240

Query: 244 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 303
           SLEKPPTRVEAVSAFLWKRFMEIN +S Q  EK++AA+HAVN+RGKMDPPLPPNSSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINPHSAQ--EKVFAAVHAVNIRGKMDPPLPPNSSGNLW 300

Query: 304 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGE 363
           WFAIAVAE E++KRHQYLV QIRNAIEEIDS+LVR LQ AGVP HMWSK GKHGH A GE
Sbjct: 301 WFAIAVAETEIEKRHQYLVNQIRNAIEEIDSDLVRKLQHAGVPSHMWSKMGKHGHAAGGE 360

Query: 364 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 423
           VEFCNFTSWCRFPVYEADFGWGKP+WVCSPSRP+KNVVVLMTTRDGDGIE WVTL E+DM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEGWVTLKEEDM 420

Query: 424 ASFQQDSELVTY 436
             FQ+DSEL+++
Sbjct: 421 ELFQRDSELLSF 430

BLAST of MS001876 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 3.1e-83
Identity = 170/423 (40.19%), Postives = 253/423 (59.81%), Query Frame = 0

Query: 9   IQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSGSRQLK 68
           +Q+ S+E+I PSSPTP  L+  KLS LDQ+  + + PII FY    D   DR   S  LK
Sbjct: 5   MQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRSENLK 64

Query: 69  KSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLHRLLPF 128
           +SLS +LT FYP AG ++    SVDCND GV + EARV  +L E +++     L++ LPF
Sbjct: 65  RSLSTVLTQFYPLAGRININS-SVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQYLPF 124

Query: 129 EPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAINRGDS 188
           +PY    E  + +D+ LAV+++ F+CGG AI +C++HK+ D LS   F+  W A  + ++
Sbjct: 125 QPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATCQEET 184

Query: 189 DQVSEPLFD-GAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAGSLE- 248
           D + +P FD G+ +FPP E   +   + + ++ IV KRFVFDK  + AL+ + A+ + E 
Sbjct: 185 D-IVQPNFDLGSHHFPPME--SIPAPEFLPDENIVMKRFVFDKEKLEALKAQLASSATEV 244

Query: 249 KPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLWWFA 308
           K  +RV+ V A +WK+F+++ R       K+ AA  AVNLR +M+PP P ++ GN+   A
Sbjct: 245 KNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVAA-QAVNLRSRMNPPFPQSAMGNIATMA 304

Query: 309 IAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGEVEF 368
            AVAE +  K    LV  ++ ++ +ID   V+ LQ          K   +    A   E 
Sbjct: 305 YAVAEED--KDFSDLVGPLKTSLAKIDDEHVKELQ----------KGVTYLDYEAEPQEL 364

Query: 369 CNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDMASF 428
            +F+SWCR   Y+ DFGWGKP  VC+ + P KN+V LM TR+ DG+EAW+++ E +M+  
Sbjct: 365 FSFSSWCRLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDGMEAWISMAEDEMSML 410

Query: 429 QQD 430
             D
Sbjct: 425 SSD 410

BLAST of MS001876 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 282.0 bits (720), Expect = 1.2e-74
Identity = 158/421 (37.53%), Postives = 241/421 (57.24%), Query Frame = 0

Query: 9   IQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSGSRQLK 68
           ++  S+E+ILPSSPTP  L+  K+S LDQ+  + + P I FY    D   D    S+ LK
Sbjct: 5   MEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDPAQTSQHLK 64

Query: 69  KSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVES-PDFKVLHRLLP 128
           +SLS++LT FYP AG ++    SVDCND GV + EARV  +L + +++  + + L + LP
Sbjct: 65  QSLSKVLTHFYPLAGRINVNS-SVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLDQYLP 124

Query: 129 FEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAINRGD 188
              Y    + E+  DV LAV+++ F+CGG AI + ++HK+ D LS   F+  W A  RG+
Sbjct: 125 SAAYPG-GKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATCRGE 184

Query: 189 SDQVSEPLFD-GAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAGSLE 248
           ++ V  P FD  A++FPP  +      +++ ++ +V KRFVFDK  I ALR +A++ S E
Sbjct: 185 TEIVL-PNFDLAARHFPP--VDNTPSPELVPDENVVMKRFVFDKEKIGALRAQASSASEE 244

Query: 249 KPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLWWFA 308
           K  +RV+ V A++WK  +++ R       K +  + AVNLR +M+PPLP  + GN+    
Sbjct: 245 KNFSRVQLVVAYIWKHVIDVTRAKYGAKNK-FVVVQAVNLRSRMNPPLPHYAMGNIATLL 304

Query: 309 IAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGEV-- 368
            A  + E  K    L+  +R ++E+ + +    L +              G     E+  
Sbjct: 305 FAAVDAEWDKDFPDLIGPLRTSLEKTEDDHNHELLK--------------GMTCLYELEP 364

Query: 369 -EFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 425
            E  +FTSWCR   Y+ DFGWGKP   C+ + P +N  +LM TR GDG+EAW+ + E +M
Sbjct: 365 QELLSFTSWCRLGFYDLDFGWGKPLSACTTTFPKRNAALLMDTRSGDGVEAWLPMAEDEM 405

BLAST of MS001876 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 8.8e-62
Identity = 147/433 (33.95%), Postives = 234/433 (54.04%), Query Frame = 0

Query: 4   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSG 63
           MA + +++ S   I PSSPTP  LR   LS +DQ   S  T + FFY    +P  +    
Sbjct: 1   MASKMVEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFY--TYNPSHESSKI 60

Query: 64  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH 123
              LK SLS+ L  +Y FAG L    Y +DCND+GVE+ E R+  ++ ++++        
Sbjct: 61  GDLLKNSLSKTLVSYYQFAGRLIENDY-IDCNDEGVEFVEVRIHGRMNDILKRGKSFATD 120

Query: 124 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI 183
            +LP    A      +  D +L V+L+ FDCGG+AI    +HK+ DG+S  +F+++WA+ 
Sbjct: 121 LVLPTRIIA------LHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWASS 180

Query: 184 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG 243
               +     PL      FPP++   L    ++   + + KRFVF    I  L+ +A   
Sbjct: 181 TSLSTFHKPTPLLTADSIFPPEDNKLLSNKSIVSFQQCLGKRFVFSTEAIEKLKSKAIEY 240

Query: 244 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 303
            ++K P+RVE V+AFL     +   N +   +K YA I AVNLR  +   LP NS GN++
Sbjct: 241 GIQK-PSRVEVVTAFL----CQCAANCDLPRKKPYAIISAVNLRPYL--ALPQNSIGNIF 300

Query: 304 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGE 363
            F   + +  M  +   L+ ++RN  +++  N++ + ++      M   T          
Sbjct: 301 SFYFCINDEGMDNQFSALISKLRNGKQKLLENII-SKEKLTYESQMQELTKCLDQLNISS 360

Query: 364 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRP-FKNVVVLMTTRDGDGIEAWVTLNEQD 423
           ++    +SWCRFPVY+ DFGWGKP  V SP +P  K++++LM + +GDGIEA +T+ E+ 
Sbjct: 361 LDTYFCSSWCRFPVYDIDFGWGKPILV-SPFQPHVKDLILLMDSPEGDGIEALITMEEKK 415

Query: 424 MASFQQDSELVTY 436
           MA+F+++ EL ++
Sbjct: 421 MAAFEKNEELRSF 415

BLAST of MS001876 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 5.3e-59
Identity = 161/472 (34.11%), Postives = 226/472 (47.88%), Query Frame = 0

Query: 4   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVED---PGADR 63
           M+   ++V S E I P +PTP  LRN  +SLLDQ S   Y PII FY A  D    G+  
Sbjct: 4   MSSAAVEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAASDANSTGSKH 63

Query: 64  FSGSRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFK 123
                 LK+SLSE L  FYP AG +  +  +VDCND+G+++ E R+  ++ + +   D  
Sbjct: 64  HDDLHLLKRSLSETLVHFYPMAGRMK-DNMTVDCNDEGIDFFEVRIKGRMCDFMMKSDAH 123

Query: 124 VLHRLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREW 183
            L  LLP E    +A     ++  + V++N+FDCGG AI  C+++K+ D  +   F+R  
Sbjct: 124 -LSLLLPSE----VASTNFVKEAQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSL 183

Query: 184 AA----INRGDS------DQVSEPLFDGAKYFPPKE-------IPGLKRSKVILNDKIVT 243
           A       RG S      +Q   P FD    FPP E        P    + V   DK+V+
Sbjct: 184 AGTTNIARRGSSIAAPTTNQNLVPSFDSTSLFPPSEQLASQVSYPTQDSTSV---DKLVS 243

Query: 244 KRFVFDKANIAALRREAAAGSLEK----PPTRVEAVSAFLWKRFMEINRNSEQENEKIYA 303
           KRFVFD A I + R +  +   +K     PTRVE VSA +WK  ++           I  
Sbjct: 244 KRFVFDAAKITSAREKLQSLMHDKYKCHRPTRVEVVSALIWKSAVK-----SAPPGSIST 303

Query: 304 AIHAVNLRGKMDPPLPPNSSGNLWWFAIAVAEVEMKKRHQYLVKQIRN-------AIEEI 363
             HA+N R KMDPPL   S GNL     AV             K++ +       A++E+
Sbjct: 304 VTHAMNFRKKMDPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDEL 363

Query: 364 D---SNLVRNLQQAGVPPHMWSKTGK---HGHPAA--------GEVEFCNFTSWCRFPVY 423
               + L R + +         K  +   +GH A+         +V     TSWC+F  Y
Sbjct: 364 SDFVALLRREIDKVKGDKGCMEKIIQKFIYGHDASVAKDSDVEDKVTALFMTSWCKFGFY 423

Query: 424 EADFGWGKPTWVCSP---SRPFKNVVVLMTTRDGDGIEAWVTLNEQDMASFQ 428
           EADFGWG P WV +       +KN+V +   + G+GIE WV   E DM  F+
Sbjct: 424 EADFGWGTPVWVTTVPLIEPKYKNMVFMNDMKCGEGIEVWVNFLEDDMTKFE 461

BLAST of MS001876 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.0e-58
Identity = 160/478 (33.47%), Postives = 233/478 (48.74%), Query Frame = 0

Query: 9   IQVTSDEIILPSSPTPPPLRNLKLSLLDQISP-SNYTPIIFFY---AAVEDPGADRFSGS 68
           ++V S E I P++PTP  L+N  LSLLDQ  P   Y PII FY   AA     ++     
Sbjct: 9   VEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDDL 68

Query: 69  RQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLHR 128
             LK SLS+ L  FYP AG +  +   VDC+D+G+ + + ++  K+ E +  PD   L +
Sbjct: 69  DLLKSSLSKTLVHFYPMAGRM-IDNILVDCHDQGINFYKVKIRGKMCEFMSQPDVP-LSQ 128

Query: 129 LLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAIN 188
           LLP E    +    + ++ ++ V++N+FDCGG AI   V+HK+ D  + + F+R WA+  
Sbjct: 129 LLPSE----VVSASVPKEALVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTT 188

Query: 189 RGD---------SDQVSEPLFDGAKYFPP----------KEIPGLKRSKVILNDKIVTKR 248
           +           +DQ   P FD A  FPP           EIP     +   +DK V+KR
Sbjct: 189 KTSRSGGSTAAVTDQKLIPSFDSASLFPPSERLTSPSGMSEIPFSSTPEDTEDDKTVSKR 248

Query: 249 FVFDKANIAALRREAAA----GSLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAI 308
           FVFD A I ++R +           +  TRVE V++ +WK  M+           +    
Sbjct: 249 FVFDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIWKSVMK-----STPAGFLPVVH 308

Query: 309 HAVNLRGKMDPPLPPNSSGNLWWFAIAV----------------------AEVEMKKRHQ 368
           HAVNLR KMDPPL   S GNL     A                       ++V + + H 
Sbjct: 309 HAVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVVLHELHD 368

Query: 369 YLVKQIRNAIEEI--DSNLVRNLQQAGVPPHMWSKTGKHGHPAA----GEVEFCNF--TS 428
           + + Q+R+ I+++  D   +  + Q              GH A+     +VE  NF  +S
Sbjct: 369 F-IAQMRSEIDKVKGDKGSLEKVIQ----------NFASGHDASIKKINDVEVINFWISS 428

BLAST of MS001876 vs. ExPASy TrEMBL
Match: A0A6J1C4D5 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111007275 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 2.8e-252
Identity = 430/432 (99.54%), Postives = 431/432 (99.77%), Query Frame = 0

Query: 4   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSG 63
           MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVED GADRFSG
Sbjct: 1   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDLGADRFSG 60

Query: 64  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH 123
           SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH 120

Query: 124 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI 183
           RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI
Sbjct: 121 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI 180

Query: 184 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG 243
           NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG
Sbjct: 181 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG 240

Query: 244 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 303
           SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 300

Query: 304 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGE 363
           WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDS+LVRNLQQAGVPPHMWSKTGKHGHPAAGE
Sbjct: 301 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSDLVRNLQQAGVPPHMWSKTGKHGHPAAGE 360

Query: 364 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 423
           VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 420

Query: 424 ASFQQDSELVTY 436
           ASFQQDSELVTY
Sbjct: 421 ASFQQDSELVTY 432

BLAST of MS001876 vs. ExPASy TrEMBL
Match: A0A6J1I7Z0 (vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111472185 PE=3 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.2e-223
Identity = 379/432 (87.73%), Postives = 402/432 (93.06%), Query Frame = 0

Query: 4   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSG 63
           MAI  IQV +DEII PSSPTPP  RNLK SLLDQISPSNYTPIIFFYAAVE+PGADRFSG
Sbjct: 1   MAIELIQVIADEIISPSSPTPPSRRNLKFSLLDQISPSNYTPIIFFYAAVENPGADRFSG 60

Query: 64  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH 123
           SRQLKKSLSEILTLFYPFAGTLS+ GYSVDCND+GVEYSEARV C+L EVVE+PDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSSAGYSVDCNDRGVEYSEARVGCELSEVVENPDFKVLH 120

Query: 124 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI 183
           RLLPFEPYAN  EPEIGRDVILAVRLNLFDCGGVAIALC+THKLVDG SATVFVREWAAI
Sbjct: 121 RLLPFEPYANSVEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180

Query: 184 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG 243
           NRGDSDQVSEPLFD AKYFPPKEIPGLKRSKV+ NDKIVTKRFVFDKANIAALR EAAAG
Sbjct: 181 NRGDSDQVSEPLFDSAKYFPPKEIPGLKRSKVVQNDKIVTKRFVFDKANIAALRHEAAAG 240

Query: 244 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 303
           SLEKPPTRVEAVSAFLWKRFMEIN +SEQ  EK++AA+HAVN+RGKMDPPLPPNSSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINPHSEQ--EKVFAAVHAVNIRGKMDPPLPPNSSGNLW 300

Query: 304 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGE 363
           WFAIAVAE E++KRHQYLV QIRNAIEEIDS+LVR LQ AGVP HMWSK GKHGH A GE
Sbjct: 301 WFAIAVAETEIEKRHQYLVNQIRNAIEEIDSDLVRKLQHAGVPSHMWSKMGKHGHAAGGE 360

Query: 364 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 423
           VEFCNFTSWCRFPVYEADFGWGKP+WVCSPSRP+KNVVVLMTTRDGDGIEAWVTL E+DM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLKEEDM 420

Query: 424 ASFQQDSELVTY 436
             FQ+DSEL+++
Sbjct: 421 ELFQRDSELLSF 430

BLAST of MS001876 vs. ExPASy TrEMBL
Match: A0A6J1EY64 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111439839 PE=3 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.3e-222
Identity = 378/432 (87.50%), Postives = 402/432 (93.06%), Query Frame = 0

Query: 4   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSG 63
           MAI  IQV +DEII PSSPTPP  RNLKLSLLDQISPSNYTPIIFFYAAVE+PGAD FSG
Sbjct: 1   MAIEFIQVIADEIISPSSPTPPSRRNLKLSLLDQISPSNYTPIIFFYAAVENPGADWFSG 60

Query: 64  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLH 123
           SRQLKKSLSEILTLFYPFAGTLS+ GYSVDCND+GVEYSEARV C+L EVVE+PDFKVLH
Sbjct: 61  SRQLKKSLSEILTLFYPFAGTLSSAGYSVDCNDRGVEYSEARVGCELSEVVENPDFKVLH 120

Query: 124 RLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI 183
           RLLPFEPYAN  EPEIGRDVILAVRLNLFDCGGVAIALC+THKLVDG SATVFVREWAAI
Sbjct: 121 RLLPFEPYANSVEPEIGRDVILAVRLNLFDCGGVAIALCITHKLVDGFSATVFVREWAAI 180

Query: 184 NRGDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAG 243
           NRGDSDQVSEPLFD AKYFPPKEIPGLKRSKV+ NDKIVTKRFVFDKANIAALR EAAAG
Sbjct: 181 NRGDSDQVSEPLFDSAKYFPPKEIPGLKRSKVVQNDKIVTKRFVFDKANIAALRHEAAAG 240

Query: 244 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 303
           SLEKPPTRVEAVSAFLWKRFMEIN +SEQ  EK++AA+HAVN+RGKMDPPLPP+SSGNLW
Sbjct: 241 SLEKPPTRVEAVSAFLWKRFMEINPHSEQ--EKVFAAVHAVNIRGKMDPPLPPSSSGNLW 300

Query: 304 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGE 363
           WFAIAVAE E++KRHQYLV QIRNAIEEIDS+LVR LQ AGVP HMWSK GKHGH A GE
Sbjct: 301 WFAIAVAETEIEKRHQYLVNQIRNAIEEIDSDLVRKLQHAGVPSHMWSKMGKHGHAAGGE 360

Query: 364 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 423
           VEFCNFTSWCRFPVYEADFGWGKP+WVCSPSRP+KNVVVLMTTRDGDGIEAWVTL E+DM
Sbjct: 361 VEFCNFTSWCRFPVYEADFGWGKPSWVCSPSRPYKNVVVLMTTRDGDGIEAWVTLKEEDM 420

Query: 424 ASFQQDSELVTY 436
             FQ+DSEL+++
Sbjct: 421 ELFQRDSELLSF 430

BLAST of MS001876 vs. ExPASy TrEMBL
Match: A0A7N2MLI3 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 2.8e-127
Identity = 228/432 (52.78%), Postives = 307/432 (71.06%), Query Frame = 0

Query: 6   IRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSGSR 65
           + N+ + S EII PSSPTP  L N KLS LDQI+P  Y PII FY + +    D F  S 
Sbjct: 2   VMNVDIISKEIIKPSSPTPHHLSNFKLSFLDQIAPPIYIPIILFYDSEKFVDVDPFEISH 61

Query: 66  QLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLHRL 125
            LKKSL+E LT +YP AGT   E + ++CND+GV+Y +AR+ C L +VV++P  K L +L
Sbjct: 62  SLKKSLAETLTRYYPLAGTPINEDFYINCNDQGVDYIQARIPCNLSQVVDNPKGKELMQL 121

Query: 126 LPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAINR 185
           LPFEPY   +  E G++V+LAV+ N+F+CGGVAIA+CV+HK+ DG SA  FV  WA ++R
Sbjct: 122 LPFEPYH--SRTEFGKEVLLAVQYNMFECGGVAIAVCVSHKIADGTSAVTFVNAWAGMSR 181

Query: 186 GDSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAA--G 245
           G+S+ +S P FD A +FPP++I G   S  +  + IVT+RFVF+K++IA LR EA+A  G
Sbjct: 182 GESEIIS-PSFDAAIHFPPRDITGSMLSGAVSKENIVTRRFVFNKSSIATLREEASAAFG 241

Query: 246 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLW 305
             +K P+RVEA+SAF+W+RFM ++R  + +  K++AA+HAVNLR +M P LP +S GNLW
Sbjct: 242 PEDKGPSRVEAISAFIWRRFMAMSRAKQGKKPKLFAALHAVNLRERMVPQLPVHSFGNLW 301

Query: 306 WFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGE 365
              IA   VE++K +++LV Q+RNAI EID++ ++ L+           T K      G 
Sbjct: 302 RIVIAALPVEIEKDYRFLVSQLRNAIMEIDADFLKKLKDDDGHLDFIKNTSKQFD--HGI 361

Query: 366 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 425
           +EFCNFTSWCRFPVYE DFG GKPTWVCSPSRPFKNVVV+++T+DGDGIE WV + E+DM
Sbjct: 362 IEFCNFTSWCRFPVYEVDFGMGKPTWVCSPSRPFKNVVVMISTKDGDGIETWVNMKEEDM 421

Query: 426 ASFQQDSELVTY 436
           A F+ D EL++Y
Sbjct: 422 ALFEHDQELLSY 428

BLAST of MS001876 vs. ExPASy TrEMBL
Match: A0A6J1CAK2 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111008950 PE=3 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 6.2e-127
Identity = 236/431 (54.76%), Postives = 302/431 (70.07%), Query Frame = 0

Query: 9   IQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSGSRQLK 68
           +++ S E I PSSPTPP  R LKLSLLDQ++P +YTP++FFY A +    D     R+LK
Sbjct: 5   VEIISTESIKPSSPTPPEQRELKLSLLDQVAPGSYTPLLFFYPAGQLDRGDHGGRCRKLK 64

Query: 69  KSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESP-DFKVLHRLLP 128
           +SL+E LT FY  AGTL  E Y V+CND+GVE+SEARV C+L +V++ P D   LHRLLP
Sbjct: 65  ESLAETLTRFYLLAGTL-VEAYLVECNDEGVEFSEARVACQLADVMDKPDDVAALHRLLP 124

Query: 129 FEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAINRGD 188
           F+P A L      RD ILAVR N FDCGGV IALCVTHK+VDG SAT F + WAA  RGD
Sbjct: 125 FDPDAVL-----DRDCILAVRYNAFDCGGVVIALCVTHKIVDGTSATTFTKAWAAACRGD 184

Query: 189 SDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAGSL-- 248
            +    P FD A+ FPP EI G  + +  + DKIVTKRF+F K+NIAAL++EA + +   
Sbjct: 185 HELTVVPTFDAAELFPPMEIRGGNKRQSRM-DKIVTKRFIFSKSNIAALKQEANSDAFLN 244

Query: 249 EKPPTRVEAVSAFLWKRFMEINRNSEQEN-EKIYAAIHAVNLRGKMDPPLPPNSSGNLWW 308
           ++PP+RVE VSAF+WKRFM + R+       K +A +HAVN+R ++ PPLPP+S GNLWW
Sbjct: 245 QRPPSRVETVSAFIWKRFMALARSKPTTTAAKRFAILHAVNIRNRLSPPLPPHSFGNLWW 304

Query: 309 FAIAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGEV 368
           FA+A A +E +K + +LV +IRNAI EID   V  LQ A        K G+  +  +GEV
Sbjct: 305 FAVAEAPIEEEKDYPFLVSKIRNAITEIDGEYVTALQDAEKAMRAKMKMGQMVY--SGEV 364

Query: 369 EFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDMA 428
           E  +FTSWC FP+YE+DFGWG+P WVCSP RP+KNVV+L+ T DG+GIEAWV L E+DM 
Sbjct: 365 ELYSFTSWCSFPIYESDFGWGRPAWVCSPGRPYKNVVLLVNTGDGEGIEAWVNLEEKDMP 424

Query: 429 SFQQDSELVTY 436
            F+ D EL+ +
Sbjct: 425 VFESDPELLAF 426

BLAST of MS001876 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 322.0 bits (824), Expect = 7.3e-88
Identity = 178/432 (41.20%), Postives = 257/432 (59.49%), Query Frame = 0

Query: 9   IQVTSDEIILPS--SPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSGSRQ 68
           I VTS E++ PS  +   PP  +  LS LDQ++P  + P +FFY             S  
Sbjct: 5   ITVTSQELVKPSPRNLNHPPCHH-HLSFLDQLAPPIFMPFLFFYH--NKTNLSDKERSDH 64

Query: 69  LKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLHRLL 128
           +K SLSEIL L+YP AG +   G  V CND GV + EA+ DC + +++E+P+   L++L 
Sbjct: 65  IKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPNELNKLH 124

Query: 129 PFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAINRG 188
           PFE +      E+  DV L V+L  F+CGG+A+ + ++HKL D LS  +FV  WAA  RG
Sbjct: 125 PFEFH------EVS-DVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFARG 184

Query: 189 DSDQVSEPLFDGAKYFPPKEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAAGSLE 248
            +D++  P FD AK FPP +I  L  +  I  + IVT+RFVF ++++ +L RE  +G+ +
Sbjct: 185 QTDEIITPSFDLAKMFPPCDIENLNMATGITKENIVTRRFVFLRSSVESL-RERFSGNKK 244

Query: 249 KPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNLWWFA 308
              TRVE +S F+W RFM  + N + +  KIY  IH VNLR + DP +P N  GN+  F+
Sbjct: 245 IRATRVEVLSVFIWSRFM-ASTNHDDKTGKIYTLIHPVNLRRQADPDIPDNMFGNIMRFS 304

Query: 309 IAVAEV---EMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGHPAAGE 368
           + V  +   E  +    LV Q+R  I +ID+  V+ LQ+     H+     +      GE
Sbjct: 305 VTVPMMIINENDEEKASLVDQMREEIRKIDAVYVKKLQEDN-RGHLEFLNKQASGFVNGE 364

Query: 369 VEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNEQDM 428
           +   +FTS C+FPVYEADFGWGKP WV S    +KN+V  + T++GDGIEAW+ L++ DM
Sbjct: 365 IVSFSFTSLCKFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDGIEAWINLDQNDM 423

Query: 429 ASFQQDSELVTY 436
           + F+ D EL+ Y
Sbjct: 425 SRFEADEELLRY 423

BLAST of MS001876 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 283.1 bits (723), Expect = 3.8e-76
Identity = 168/435 (38.62%), Postives = 252/435 (57.93%), Query Frame = 0

Query: 9   IQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSGSRQLK 68
           + V S +II PSSPTP  L+  KLSLL+Q+ P+ + P++FFY+A  +         + LK
Sbjct: 3   VDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSA--NNSIKPTEQLQMLK 62

Query: 69  KSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVLHRLLPF 128
           KSLSE LT FYP AG L     S+DCND G ++ EARV+  L  ++  P    L +L+P 
Sbjct: 63  KSLSETLTHFYPLAGRLK-GNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQLIP- 122

Query: 129 EPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAI-NRGD 188
                  +    R  +L  + + F+CG ++I +C++HKL D  S  +F++ WAAI +RG 
Sbjct: 123 ----TSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGS 182

Query: 189 SDQVSEPLFDGAKYFPPKEIPGLKRSKV----ILNDKIVTKRFVFDKANIAALRREAAAG 248
              +  P+FD  K FPP        + V    I+ ++ ++KRF+FD ++I AL+ +A++ 
Sbjct: 183 IKTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSKRFIFDSSSIQALQAKASSF 242

Query: 249 SLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNL- 308
            + + PTRVEAVSA +WK  M+  R     ++    A ++V+LR ++ PP   NS GNL 
Sbjct: 243 EVNQ-PTRVEAVSALIWKSAMKATRTVSGTSKPSILA-NSVSLRSRVSPPFTKNSIGNLV 302

Query: 309 WWFAIAVAEVEMKKRHQYLVKQIRNAIEEI-DSNLVRNLQQAGVPPHMWSKTGKHGHP-A 368
            +FA    E   + + Q LV +IR A +   D ++ + +        + S   + G   A
Sbjct: 303 SYFAAKAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSYQKEAGDMIA 362

Query: 369 AGEVEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRPFKNVVVLMTTRDGDGIEAWVTLNE 428
           +G+ +F  F+S CRF +YE DFGWGKP WV  PS   KN+V L+ T++  GIEAWV LNE
Sbjct: 363 SGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEAGGIEAWVNLNE 422

Query: 429 QDMASFQQDSELVTY 436
           Q+M  F+QD EL+ +
Sbjct: 423 QEMNLFEQDRELLQF 427

BLAST of MS001876 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 261.9 bits (668), Expect = 9.0e-70
Identity = 169/436 (38.76%), Postives = 242/436 (55.50%), Query Frame = 0

Query: 7   RNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDP----GADRFS 66
           +N+++ S EI+ PSSPTP   R L LSLLD +S   YT  + FYAA  DP    G     
Sbjct: 3   KNVEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAA--DPQNLLGFSTEE 62

Query: 67  GSRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARVDCKLLEVVESPDFKVL 126
            S +LKKSLS+ L +FYP AG +   G  V+CND+G  + EARVD  L E ++ P  + L
Sbjct: 63  TSLKLKKSLSKTLPIFYPLAGRII--GSFVECNDEGAVFIEARVDHLLSEFLKCPVPESL 122

Query: 127 HRLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAA 186
             L+P E  +     E     +L ++ N F CGG+ I +CV+HK+ D  S  +F+R WA 
Sbjct: 123 ELLIPVEAKSR----EAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAE 182

Query: 187 INRGDSDQVSEPLFDGAKYFPP--KEIPG--LKRSKVILNDKIVTKRFVFDKANIAALRR 246
            +RG    +  P F  ++ FP    E+P   + R + +     VTKRFVFD + I  LR 
Sbjct: 183 SSRGLGITLI-PSFTASEVFPKPLDELPSKPMDRKEEVEEMSCVTKRFVFDASKIKKLRA 242

Query: 247 EAAAGSLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNS 306
           +A+  +L K PTRVEAV+A  W+   +++R S          I  VNLRGK+D  L  N+
Sbjct: 243 KASR-NLVKNPTRVEAVTALFWRCVTKVSRLSSLTPRTSVLQI-LVNLRGKVD-SLCENT 302

Query: 307 SGNLWWFAIAVAEVEMKKRHQYLVKQIRNAIEEIDSNLVRNLQQAGVPPHMWSKTGKHGH 366
            GN+    I   E    +R Q +V +IR A E    N     + +     +  + GK  +
Sbjct: 303 IGNMLSLMILKNEEAAIERIQDVVDEIRRAKEIFSLNCKEMSKSSSRIFELLEEIGK-VY 362

Query: 367 PAAGEVEFCNFTSWCRFPVYEADFGWGKPTWVCS-PSRPFKNVVVLMTTRDGDGIEAWVT 426
               E++     SWC+  +Y+ADFGWGKP WV    +  FKN+++L+ T+DG+GIEAW+T
Sbjct: 363 GRGNEMDLWMSNSWCKLGLYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEGIEAWIT 422

Query: 427 LNEQDMASFQQDSELV 434
           L E+ M+ F+ D EL+
Sbjct: 423 LTEEQMSLFECDQELL 425

BLAST of MS001876 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 258.1 bits (658), Expect = 1.3e-68
Identity = 159/450 (35.33%), Postives = 249/450 (55.33%), Query Frame = 0

Query: 9   IQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSGSRQLK 68
           ++  S EII PSSPTP  L+ L+LS+ D I P  YT    FY   ++    +   S +LK
Sbjct: 8   VETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFY--TKNDLISQEHTSHKLK 67

Query: 69  KSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARV-DCKLLEVVESPDFKVLHRLLP 128
            SLSE LT FYP AG ++  G +VDC D+G  + +ARV +C L E ++ PDF  L +LLP
Sbjct: 68  TSLSETLTKFYPLAGRIT--GVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQLLP 127

Query: 129 FEPYANLAEPEIGRDV--ILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAAINR 188
            +   N   P +      +L V+   F CGG+AI +C+THK+ D  S + F+R WAA  R
Sbjct: 128 LDVVDN---PYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATAR 187

Query: 189 GDSD--QVSEPLFDGAKYFPP-KEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREAAA 248
           G++D   +  P+F GA ++PP  E   L   +       +TKRFVF+ + +  LR +AA+
Sbjct: 188 GENDAAAMESPVFAGANFYPPANEAFKLPADEQAGKRSSITKRFVFEASKVEDLRTKAAS 247

Query: 249 GSLEKPPTRVEAVSAFLWKRFMEINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSSGNL 308
                 PTRVE+V+A +WK F+  ++ +  +++ +   +   NLR K+   L  +S GNL
Sbjct: 248 EETVDQPTRVESVTALIWKCFVASSKTTTCDHKVL---VQLANLRSKIPSLLQESSIGNL 307

Query: 309 WWFAIAVA-----EVEMKKRHQYLVKQIRNAIEEI-----------DSNLVRNLQQAGVP 368
            + ++ ++     EV++++     V+ +R   EE+           DS+ +   + A + 
Sbjct: 308 MFSSVVLSIGRGGEVKIEE----AVRDLRKKKEELGTVILDEGGSSDSSSMIGSKLANLM 367

Query: 369 PHMWSKTGKHGHPAAGEVEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRP-FKNVVVLMT 428
              +S+     H      E    +SWC+ P+YEA FGW  P WV     P   N+ +L+ 
Sbjct: 368 LTNYSRLSYETH------EPYTVSSWCKLPLYEASFGWDSPVWVVGNVSPVLGNLAMLID 427

Query: 429 TRDGDGIEAWVTLNEQDMASFQQDSELVTY 436
           ++DG GIEA+VTL E++M+SF+Q+ EL+ +
Sbjct: 428 SKDGQGIEAFVTLPEENMSSFEQNPELLAF 437

BLAST of MS001876 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 252.3 bits (643), Expect = 7.1e-67
Identity = 159/447 (35.57%), Postives = 249/447 (55.70%), Query Frame = 0

Query: 4   MAIRNIQVTSDEIILPSSPTPPPLRNLKLSLLDQISPSNYTPIIFFYAAVEDPGADRFSG 63
           M    ++  + EII PSS TP  L+ L+LS+ D I P  YT    FY   +D        
Sbjct: 1   METMKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFY--TKDDLISPEQS 60

Query: 64  SRQLKKSLSEILTLFYPFAGTLSTEGYSVDCNDKGVEYSEARV-DCKLLEVVESPDFKVL 123
           S +LK SL+E LT FYP AG +  +G ++DCND+G  + +ARV +  L + + SPDFK L
Sbjct: 61  SHKLKTSLAETLTKFYPLAGRI--KGVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTL 120

Query: 124 HRLLPFEPYANLAEPEIGRDVILAVRLNLFDCGGVAIALCVTHKLVDGLSATVFVREWAA 183
            +LLP +   +  E      ++L V+   F CGG+AI LC+THK+ D  S + F++ WAA
Sbjct: 121 QQLLPLDVIDDPYEAATTWPLLL-VKATYFPCGGMAIGLCITHKIADATSISTFIQSWAA 180

Query: 184 INRGDS-DQVSEPLFDGAKYFPP-KEIPGLKRSKVILNDKIVTKRFVFDKANIAALRREA 243
           + RG++ D V++P+F  A ++PP  E+      +       +TKRFVF  + +  LR +A
Sbjct: 181 MARGEAGDIVADPVFAAANFYPPANELFKFPVDENANKISQITKRFVFSASKLEELRTKA 240

Query: 244 AAGSLEKPPTRVEAVSAFLWKRFM-EINRNSEQENEKIYAAIHAVNLRGKMDPPLPPNSS 303
           A+      P RVE+V+A LWK F+   + N+   + K+   I   NLR K+   L  +  
Sbjct: 241 ASEDFVGRPKRVESVTALLWKAFVAAASSNTNACDMKV--LIQPSNLRPKIPSLLAESLI 300

Query: 304 GNLWWFAIAVA-----EVEMKKRHQYLVKQ---IRNAIEEI--DSNLVRNLQQAGVPPHM 363
           GN+ + ++ +      E++++K  + L KQ   ++N I+E    S+ +   + A +    
Sbjct: 301 GNIMFSSVVLGISQQEEIKIEKAVRDLRKQGDDLQNVIKEEGGSSSAMIGSKLANLMLTN 360

Query: 364 WSKTGKHGHPAAGEVEFCNFTSWCRFPVYEADFGWGKPTWVCSPSRP-FKNVVVLMTTRD 423
           +SK     H      E    +SWC+ P+YEA FGWG P W+     P  +NV +L+ ++D
Sbjct: 361 YSKLSYETH------EPYTVSSWCKLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDSKD 420

Query: 424 GDGIEAWVTLNEQDMASFQQDSELVTY 436
           G GIEA+VTL E++M S +Q++EL+ +
Sbjct: 421 GQGIEAFVTLPEENMLSLEQNTELLAF 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135273.15.7e-25299.54vinorine synthase-like [Momordica charantia][more]
KAG6587429.18.6e-22487.96CBBY-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022973602.12.5e-22387.73vinorine synthase-like [Cucurbita maxima][more]
XP_022933091.12.8e-22287.50vinorine synthase-like [Cucurbita moschata][more]
XP_023531342.12.3e-22187.04vinorine synthase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A2P1GIW73.1e-8340.19Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
Q70PR71.2e-7437.53Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2[more]
A0A2K8FQU58.8e-6233.95Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT... [more]
I3PLR45.3e-5934.11(13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver ... [more]
Q94FT42.0e-5833.47Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1C4D52.8e-25299.54vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111007275 PE=3 SV=1[more]
A0A6J1I7Z01.2e-22387.73vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111472185 PE=3 SV=1[more]
A0A6J1EY641.3e-22287.50vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111439839 PE=3 SV=1[more]
A0A7N2MLI32.8e-12752.78Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
A0A6J1CAK26.2e-12754.76vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111008950 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G24430.17.3e-8841.20HXXXD-type acyl-transferase family protein [more]
AT3G26040.13.8e-7638.62HXXXD-type acyl-transferase family protein [more]
AT1G24420.19.0e-7038.76HXXXD-type acyl-transferase family protein [more]
AT4G15390.11.3e-6835.33HXXXD-type acyl-transferase family protein [more]
AT3G30280.17.1e-6735.57HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003480TransferasePFAMPF02458Transferasecoord: 9..432
e-value: 7.1E-83
score: 278.7
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 218..434
e-value: 6.2E-68
score: 230.1
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 6..208
e-value: 3.6E-58
score: 198.4
NoneNo IPR availablePANTHERPTHR31623:SF25VINORINE SYNTHASE-LIKEcoord: 7..434
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 7..434

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001876.1MS001876.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups