MS001786 (gene) Bitter gourd (TR) v1

Overview
NameMS001786
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncarbonyl reductase [NADPH] 1-like
Locationscaffold30: 46786 .. 49960 (+)
RNA-Seq ExpressionMS001786
SyntenyMS001786
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCACGAAGGAAAGGGCCAAGGAACGAAGGGAAAAGAGAATGCAAGAAATATCCCTTCTCAGGACCATTCCTTACTCCGATCACCAGAGGTCTGAATGTATTACTTAATGACACACATTGTTATTTTACACCTACCATCTTAATTCTTTCTTCGTTGCCCATAACTCATAACTGAATCACGTGTTATCGGAAACCCATTTATTCTTTATTAGTATTGCTGAAAGTAAAGTACAATCGATTACCTTCACTCCTTATATGTATTATATAGGACTAGATTCAATCTCTTCGGATCCGATATCGAAATGTGTCCTCCAAATTATCGCATGGATTAAATTACCTGATGAAACCAGTCTGTTTACCCGTAATTTACTTCTACTTGCCCTAATTTTTTATTTAAAGATGCTATTCTTAGTACGAGACATTTTCATTTTTGTTCTGTAATAGTTCTCTTAGTATTTTACTTGTAGATATTCCATGTTCTATATTATTGCGTGCAACTGAAACTGAAGAGATAACATAAATCTATATCCATTTGATATTAAATTCTTCATGGCAACCACTTTATAGTTTTCTTCTTGCATTTCCTTTCTTTAACCATGCCTTCGGACGACGTCGTATTTTATGGAACCAAGGTGCTTGGCTGTTTAAATGATTTAAAAATTTCCTACAAATTCATTTCATCAAGGAGATGATGCTGTACTTGTTTCCAATTTTTAACGCGTTCCTTGTATCATTTCATTTGTCTCATTTGTTTCTCTCTTTCTATTGGCAACTTTTTTTCTGGACAGGAACCAACGCCCACATTTTACATTTCCTTGACTTTTTCTTGTAGGTGGTGGTCTACAGAAACCATTGCTGTGGTTACCGGTGGTAATAGAGGAATTGGATTTGAGATTTCAAGACAATTTGCAATGCATGGGTTGACCGTCATCTTGACATCAAGAGATGTTTCTGTTGGCCTTGAAGCAGCTAAGGTCTTACAGGAAGGTGGTCTGAATGTTGACTTTCATCAACTGGATGTCTTGGATGCTTTATCCATCAAACAGTTTGCTGATTGGCTGCTACAAAACTATGGTGGCTTGGATATTCTGGCAAGTTAATTTTTGCACTGAACTTTTCTTTGTATTGATTTTTTGAATTGGCTAAAAAGAAGAGCACGTATATTTTGTGCGGTGTGATCCAACTTTGACAAGCAACATGCTGAATGCTAACTATATATTGAAATCAACAAAAGCAATAGTGCTGAAAATATTCAAGAACATGATAATTATTGTTGCTACAGATTTTGGTGACATAATTTTCAAATGCCCCAACTACGTGCAAATATTTTTGCAGATAAATAATGCTGGAGTAAATTTCAATCTTGGGTCTAGCAATTCTGTAGAGTTTGCTCAGATGGTCATTTCTACCAACTATTTTGGCACCAAAAATATGATACAAGCAATGATTCCCTTGATGAAACCGTCAGCTGCTGGTGCTCGCATTGTTAATGTCAGCTCAAGGTTGGGCAAGCTGAATGGCAGGCGGAATGTAAGTTGAAATTCTTCCTGTTTTACCTGAAGTTCTTGTCTTCTCTATATCATGGGTGGATTGAGTACATTTAATATAATGAATTAGGGGTGTTGATGGGTTGAGTTAGCTTGGATTGAAGTACTTTTTAGGCTCAACTTAATTGTATGTTTGGGATGTAAATTTATTCAACAATCCTTATTAAATAATGAATGCATCTGAAATATTTGGGTTTTTTGGTTCAGGTTATTTGGGTCACTCATTCATAAATATTTATTTACATATTTTCTAACATTGAATCAAGATCCGTATGGTGAAAAATTACCATCCAAAAGCCCCCAAGGAGCTGTCTCGTTCTCTGTTGGCAAGGGCTTGAGGTCTCTTAGTCGTACTGGTTTAGAGGTCTCGAGTTTCAACCTCCGGGTGAGTTTAATACCAAAAATTTAATCTCTCCTTGATCCTTGAGGTGAGTGCAGGTCCCCGGGGATAGGGGAGCAAAACACCGATTCCCAATTATAAATATAAATATAAATATATATATATATATATATATATATAAAATCCAAAAACTTAAAAAAGTTATTTTTTGTAGTTGTTGGATAACAAATAATGCATAAAGGATTGTTGAATGAAATAAAATTAAAATAAATATAACCATATAAAATTGTATGGTAAGTAAAAGAAATAATTTCAAAATTAGTAATAAGTCTAGTTTGGTTTGGTTTCATTCAACTCAAATTTATTTTAGGTAAACCTTTGAACCAACCTAACTATATAGAGTTTTAATTCATTTGAAACCAACTCAACTCAAATTGTCTGTATTAGGTTGGATTGATCGCGTTTTATTTACCATCCCTGTTTGAATAATTATATCTTGTTTTGTCTAAATATTGAAATATAGGCATTCGCTATGATTGTTGATGGTATTATGCACTCAAAGAGATTGTTCTTTCTCCTTTATGTGGATTGACGATCGTCCACTTGCATTTTATATTGTTCTTTTTTTTTCTCTTCTTTTTTTTTTGTACTTCTTCTTTGGGGGAGAGTGTGGGGGTGGAGGTGTCTGATCTGCTGCTCCCTAGTTTCAACGCCCCCTACAAGAAATAATGTTAGATGATATTATTTTGAGCAAGGTTAGTTGTTGGGTTGGTGCTAACAGCCAATGCATTCTCAATTGTGATAATGACTGCAAATCATATGTTTTTAAATATTATCATGATAGTTTTTCACGCTCATCTTTCCTCCATGCTAATTTATGGTTTAATTACAGAGACTGGAAAATGTAGCAGTTAGAGAACTACTTAGCAATTTGGATACACTCTCAGAGGAAGTAATTGATAGGTTCGTGTCTACCTTTTTGCAACAAGTTGAAGATGGAAGTTGGGAAACTGGCGGGTGGCCTCAGTCGTTGACTGACTACTCTGTATCAAAACTTGCAGTTAATGCGTACACTAGGTTAATGGCAAAAATATTCACTGACCGACCCGAGGGTCATAAGATTTATGTGAACTGCTACTGCCCTGGTTGGGTGAAAACTCCTATGACAGGATTCGCTGGGAATGTTTCAGCTGAGGAAGGGGCTGACACTGGTGTATGGCTTACCTTACTTCCAGATCAGGCAGTAACAGGTAAATTTTTTGCTGAGCGACGGGAGATAAGCTTC

mRNA sequence

ATGGGCACGAAGGAAAGGGCCAAGGAACGAAGGGAAAAGAGAATGCAAGAAATATCCCTTCTCAGGACCATTCCTTACTCCGATCACCAGAGGTGGTGGTCTACAGAAACCATTGCTGTGGTTACCGGTGGTAATAGAGGAATTGGATTTGAGATTTCAAGACAATTTGCAATGCATGGGTTGACCGTCATCTTGACATCAAGAGATGTTTCTGTTGGCCTTGAAGCAGCTAAGGTCTTACAGGAAGGTGGTCTGAATGTTGACTTTCATCAACTGGATGTCTTGGATGCTTTATCCATCAAACAGTTTGCTGATTGGCTGCTACAAAACTATGGTGGCTTGGATATTCTGGCAAATTTTGGTGACATAATTTTCAAATGCCCCAACTACGTGCAAATATTTTTGCAGATAAATAATGCTGGAGTAAATTTCAATCTTGGGTCTAGCAATTCTGTAGAGTTTGCTCAGATGGTCATTTCTACCAACTATTTTGGCACCAAAAATATGATACAAGCAATGATTCCCTTGATGAAACCGTCAGCTGCTGGTGCTCGCATTGTTAATGTCAGCTCAAGGTTGGGCAAGCTGAATGGCAGGCGGAATAGACTGGAAAATGTAGCAGTTAGAGAACTACTTAGCAATTTGGATACACTCTCAGAGGAAGTAATTGATAGGTTCGTGTCTACCTTTTTGCAACAAGTTGAAGATGGAAGTTGGGAAACTGGCGGGTGGCCTCAGTCGTTGACTGACTACTCTGTATCAAAACTTGCAGTTAATGCGTACACTAGGTTAATGGCAAAAATATTCACTGACCGACCCGAGGGTCATAAGATTTATGTGAACTGCTACTGCCCTGGTTGGGTGAAAACTCCTATGACAGGATTCGCTGGGAATGTTTCAGCTGAGGAAGGGGCTGACACTGGTGTATGGCTTACCTTACTTCCAGATCAGGCAGTAACAGGTAAATTTTTTGCTGAGCGACGGGAGATAAGCTTC

Coding sequence (CDS)

ATGGGCACGAAGGAAAGGGCCAAGGAACGAAGGGAAAAGAGAATGCAAGAAATATCCCTTCTCAGGACCATTCCTTACTCCGATCACCAGAGGTGGTGGTCTACAGAAACCATTGCTGTGGTTACCGGTGGTAATAGAGGAATTGGATTTGAGATTTCAAGACAATTTGCAATGCATGGGTTGACCGTCATCTTGACATCAAGAGATGTTTCTGTTGGCCTTGAAGCAGCTAAGGTCTTACAGGAAGGTGGTCTGAATGTTGACTTTCATCAACTGGATGTCTTGGATGCTTTATCCATCAAACAGTTTGCTGATTGGCTGCTACAAAACTATGGTGGCTTGGATATTCTGGCAAATTTTGGTGACATAATTTTCAAATGCCCCAACTACGTGCAAATATTTTTGCAGATAAATAATGCTGGAGTAAATTTCAATCTTGGGTCTAGCAATTCTGTAGAGTTTGCTCAGATGGTCATTTCTACCAACTATTTTGGCACCAAAAATATGATACAAGCAATGATTCCCTTGATGAAACCGTCAGCTGCTGGTGCTCGCATTGTTAATGTCAGCTCAAGGTTGGGCAAGCTGAATGGCAGGCGGAATAGACTGGAAAATGTAGCAGTTAGAGAACTACTTAGCAATTTGGATACACTCTCAGAGGAAGTAATTGATAGGTTCGTGTCTACCTTTTTGCAACAAGTTGAAGATGGAAGTTGGGAAACTGGCGGGTGGCCTCAGTCGTTGACTGACTACTCTGTATCAAAACTTGCAGTTAATGCGTACACTAGGTTAATGGCAAAAATATTCACTGACCGACCCGAGGGTCATAAGATTTATGTGAACTGCTACTGCCCTGGTTGGGTGAAAACTCCTATGACAGGATTCGCTGGGAATGTTTCAGCTGAGGAAGGGGCTGACACTGGTGTATGGCTTACCTTACTTCCAGATCAGGCAGTAACAGGTAAATTTTTTGCTGAGCGACGGGAGATAAGCTTC

Protein sequence

MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHGLTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDILANFGDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPSAAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWETGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVSAEEGADTGVWLTLLPDQAVTGKFFAERREISF
Homology
BLAST of MS001786 vs. NCBI nr
Match: XP_022135225.1 (carbonyl reductase [NADPH] 1-like [Momordica charantia])

HSP 1 Score: 614.4 bits (1583), Expect = 5.8e-172
Identity = 313/332 (94.28%), Postives = 313/332 (94.28%), Query Frame = 0

Query: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60
           MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG
Sbjct: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60

Query: 61  LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDILANF 120
           LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDIL   
Sbjct: 61  LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDIL--- 120

Query: 121 GDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS 180
                           INNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS
Sbjct: 121 ----------------INNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS 180

Query: 181 AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE 240
           AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE
Sbjct: 181 AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE 240

Query: 241 TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS 300
           TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS
Sbjct: 241 TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS 300

Query: 301 AEEGADTGVWLTLLPDQAVTGKFFAERREISF 333
           AEEGADTGVWLTLLPDQAVTGKFFAERREISF
Sbjct: 301 AEEGADTGVWLTLLPDQAVTGKFFAERREISF 313

BLAST of MS001786 vs. NCBI nr
Match: KAG7023020.1 (Salutaridine reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.4 bits (1526), Expect = 2.4e-165
Identity = 296/332 (89.16%), Postives = 307/332 (92.47%), Query Frame = 0

Query: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60
           MG KER  E REKRMQEISL RTIPYSDHQRWWSTETIAVVTGGNRGIGFE+SRQFAMHG
Sbjct: 1   MGRKERGNELREKRMQEISLTRTIPYSDHQRWWSTETIAVVTGGNRGIGFEVSRQFAMHG 60

Query: 61  LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDILANF 120
           +TVILTSRDV VGLEAAKVLQEGGLNV FHQLDVLDALSIKQFADWLLQNYGGLDILAN 
Sbjct: 61  MTVILTSRDVCVGLEAAKVLQEGGLNVAFHQLDVLDALSIKQFADWLLQNYGGLDILAN- 120

Query: 121 GDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS 180
            DI  KC  YVQI LQINNAGVNFNLGSSNSVE+AQMVI+TNY+GTKNM QAM+PLMKPS
Sbjct: 121 -DITSKCTIYVQICLQINNAGVNFNLGSSNSVEYAQMVIATNYYGTKNMTQAMVPLMKPS 180

Query: 181 AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE 240
           +AGARIVNVSSRL KLNGRRNR+ENVA RELL NLDTL+EEVIDR VSTFL+QVEDGSWE
Sbjct: 181 SAGARIVNVSSRLAKLNGRRNRVENVAFRELLGNLDTLTEEVIDRIVSTFLEQVEDGSWE 240

Query: 241 TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS 300
           TGGWPQ  TDYSVSKLAVNAYTRLMAKI  DRPEGHKIYVNCYCPGWVKT MTGFAGN+S
Sbjct: 241 TGGWPQLSTDYSVSKLAVNAYTRLMAKILGDRPEGHKIYVNCYCPGWVKTAMTGFAGNIS 300

Query: 301 AEEGADTGVWLTLLPDQAVTGKFFAERREISF 333
           AEEGADTGVWL LLPDQAVTGKFFAERREISF
Sbjct: 301 AEEGADTGVWLALLPDQAVTGKFFAERREISF 330

BLAST of MS001786 vs. NCBI nr
Match: KAG7021365.1 (Short-chain dehydrogenase/reductase 2b, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.0 bits (1525), Expect = 3.1e-165
Identity = 300/335 (89.55%), Postives = 311/335 (92.84%), Query Frame = 0

Query: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60
           MG KERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQ AMHG
Sbjct: 1   MGKKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQLAMHG 60

Query: 61  LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDILA-N 120
           +TVILTSRDVSVGLEAAKVLQEGGLNV FHQLDVLDALSIKQFADWLLQNYGGLDILA +
Sbjct: 61  MTVILTSRDVSVGLEAAKVLQEGGLNVAFHQLDVLDALSIKQFADWLLQNYGGLDILAID 120

Query: 121 FGDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKP 180
             DI  KCP YVQ+ LQINNAGVNFNLGSSNSVEFAQMVI+TNY+GTKNM QAMIPLMKP
Sbjct: 121 LDDITSKCPIYVQLCLQINNAGVNFNLGSSNSVEFAQMVIATNYYGTKNMTQAMIPLMKP 180

Query: 181 SAAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSW 240
           S+AGARIVNVSSRLGKLNGRRNR+ENVA R LLSNLDTL+EE IDR +STFLQQVEDGSW
Sbjct: 181 SSAGARIVNVSSRLGKLNGRRNRVENVAFRGLLSNLDTLTEEAIDRMLSTFLQQVEDGSW 240

Query: 241 ETGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNV 300
           ETGGWPQ  TDYS+SKLAVNAYTRLMAK FTD+PEGHKIYVNCYCPGWVKT MTGFAGNV
Sbjct: 241 ETGGWPQLSTDYSMSKLAVNAYTRLMAKKFTDQPEGHKIYVNCYCPGWVKTAMTGFAGNV 300

Query: 301 SAEEGADTGVWLTLLPDQ--AVTGKFFAERREISF 333
           SAE GADTGVWL LLPDQ  AV GKFFAERREISF
Sbjct: 301 SAEVGADTGVWLALLPDQAVAVAGKFFAERREISF 335

BLAST of MS001786 vs. NCBI nr
Match: XP_038880046.1 (carbonyl reductase [NADPH] 1-like [Benincasa hispida])

HSP 1 Score: 584.7 bits (1506), Expect = 5.0e-163
Identity = 296/332 (89.16%), Postives = 304/332 (91.57%), Query Frame = 0

Query: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60
           MG KERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG
Sbjct: 1   MGRKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60

Query: 61  LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDILANF 120
           +TVILTSRDVSVG+EAAKVLQEGGLNV FHQLDVLDALSIKQFADWLLQNYGGLDIL   
Sbjct: 61  MTVILTSRDVSVGIEAAKVLQEGGLNVAFHQLDVLDALSIKQFADWLLQNYGGLDIL--- 120

Query: 121 GDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS 180
                           INNAGVNFNLGSSNSVEFAQMVI+TNY+GTKNMIQAMIPLMKPS
Sbjct: 121 ----------------INNAGVNFNLGSSNSVEFAQMVIATNYYGTKNMIQAMIPLMKPS 180

Query: 181 AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE 240
           +AGARIVNVSSRLGKLNGRRNR+ENVA RELLSNLDTL+EEVIDR VSTFLQQVEDG+WE
Sbjct: 181 SAGARIVNVSSRLGKLNGRRNRVENVAFRELLSNLDTLTEEVIDRIVSTFLQQVEDGNWE 240

Query: 241 TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS 300
           TGGWPQ  TDYSVSKLAVNAYTRLMAKIF DRPEGHKIYVNCYCPGWVKT MTGFAGNVS
Sbjct: 241 TGGWPQLSTDYSVSKLAVNAYTRLMAKIFADRPEGHKIYVNCYCPGWVKTAMTGFAGNVS 300

Query: 301 AEEGADTGVWLTLLPDQAVTGKFFAERREISF 333
           AEEGADTGVWL LLPDQAVTGKFFAERREISF
Sbjct: 301 AEEGADTGVWLALLPDQAVTGKFFAERREISF 313

BLAST of MS001786 vs. NCBI nr
Match: ADN33869.1 (short-chain dehydrogenase/reductase family protein [Cucumis melo subsp. melo])

HSP 1 Score: 582.4 bits (1500), Expect = 2.5e-162
Identity = 293/322 (90.99%), Postives = 303/322 (94.10%), Query Frame = 0

Query: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60
           MG KERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG
Sbjct: 1   MGKKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60

Query: 61  LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDILANF 120
           +TVILTSRDV VGLEAAKVLQEGGLNV FHQLDVLDALSIKQFADWLLQNYGGLDILAN 
Sbjct: 61  MTVILTSRDVCVGLEAAKVLQEGGLNVAFHQLDVLDALSIKQFADWLLQNYGGLDILAN- 120

Query: 121 GDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS 180
            DI  K P YVQ+  QINNAGVNFNLGSSNSVEFAQMVI+TNY+GTKNMIQAMIPLMKPS
Sbjct: 121 -DITSKFPIYVQLCPQINNAGVNFNLGSSNSVEFAQMVIATNYYGTKNMIQAMIPLMKPS 180

Query: 181 AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE 240
           +AGARIVNVSSRLGKLNGRRNR+ENV  RELLSNLDTL+EEVIDR VSTFLQQVEDGSWE
Sbjct: 181 SAGARIVNVSSRLGKLNGRRNRVENVEFRELLSNLDTLTEEVIDRIVSTFLQQVEDGSWE 240

Query: 241 TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS 300
           TGGWPQ  TDYSVSKLAVNAYTRLMAK FT+RPEGHKIYVNCYCPGWVKT MTGFAGN+S
Sbjct: 241 TGGWPQLSTDYSVSKLAVNAYTRLMAKKFTERPEGHKIYVNCYCPGWVKTAMTGFAGNIS 300

Query: 301 AEEGADTGVWLTLLPDQAVTGK 323
           AEEGADTGVWL LLPDQAVTG+
Sbjct: 301 AEEGADTGVWLALLPDQAVTGR 320

BLAST of MS001786 vs. ExPASy Swiss-Prot
Match: Q9ZUH5 (Short-chain dehydrogenase/reductase 2b OS=Arabidopsis thaliana OX=3702 GN=SDR2b PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.4e-45
Identity = 114/297 (38.38%), Postives = 170/297 (57.24%), Query Frame = 0

Query: 39  AVVTGGNRGIGFEISRQFAMHGLTVILTSRDVSVGLEAAKVLQE----GGLNVDFHQLDV 98
           A+VTGGNRGIGFEI RQ A  G+ VILTSRD   GLEA + L++       ++ FHQLDV
Sbjct: 9   AIVTGGNRGIGFEICRQLANKGIRVILTSRDEKQGLEAVETLKKELEISDQSIVFHQLDV 68

Query: 99  LDALSIKQFADWLLQNYGGLDILANFGDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEF 158
            D +S+   A+++  ++G LDIL N   +     +   +       G  +    + + E 
Sbjct: 69  SDPVSVTSLAEFVKTHFGKLDILINNAGVGGVITDVDALRAGTGKEGFKWEETITETYEL 128

Query: 159 AQMVISTNYFGTKNMIQAMIPLMKPSAAGARIVNVSSRLGKLNGRRNRLENVAVRELLSN 218
           A+  I  NY+G K M +A IPL++ S +  RI+NVSS +G++      L N   + +LS+
Sbjct: 129 AEECIKINYYGPKRMCEAFIPLLQLSDS-PRIINVSSFMGQV----KNLVNEWAKGILSD 188

Query: 219 LDTLSEEVIDRFVSTFLQQVEDGSWETGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPE 278
            + L+E  ID+ ++  L  +++ + +T  W + ++ Y VSK  +NAYTR++AK     PE
Sbjct: 189 AENLTEVRIDQVINQLLNDLKEDTAKTKYWAKVMSAYVVSKAGLNAYTRILAK---KHPE 248

Query: 279 GHKIYVNCYCPGWVKTPMTGFAGNVSAEEGADTGVWLTLLPDQAVTGKFFAERREIS 332
              I VN  CPG+VKT M    G +S EEGA + V L LLP Q      F +R+++S
Sbjct: 249 ---IRVNSVCPGFVKTDMNFKTGILSVEEGASSPVRLALLPHQESPSGCFFDRKQVS 294

BLAST of MS001786 vs. ExPASy Swiss-Prot
Match: Q9M2E2 ((+)-neomenthol dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=SDR1 PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 7.4e-45
Identity = 116/301 (38.54%), Postives = 167/301 (55.48%), Query Frame = 0

Query: 35  TETIAVVTGGNRGIGFEISRQFAMHGLTVILTSRDVSVGLEAAKVLQE----GGLNVDFH 94
           T   AVVTG NRGIGFEI RQ A  G+ V+LTSRD + GLEA + L++       ++ FH
Sbjct: 5   TPRYAVVTGANRGIGFEICRQLASEGIRVVLTSRDENRGLEAVETLKKELEISDQSLLFH 64

Query: 95  QLDVLDALSIKQFADWLLQNYGGLDILANFGDIIFKCPNYVQIFLQINNAGVNFNLGSSN 154
           QLDV D  SI   A+++   +G LDIL N   I     +   +       G  ++   + 
Sbjct: 65  QLDVADPASITSLAEFVKTQFGKLDILVNNAGIGGIITDAEALRAGAGKEGFKWDEIITE 124

Query: 155 SVEFAQMVISTNYFGTKNMIQAMIPLMKPSAAGARIVNVSSRLGKLNGRRNRLENVAVRE 214
           + E  +  I  NY+G K M +A IPL+K S +  RIVNVSS +G+L      + N   + 
Sbjct: 125 TYELTEECIKINYYGPKRMCEAFIPLLKLSDS-PRIVNVSSSMGQL----KNVLNEWAKG 184

Query: 215 LLSNLDTLSEEVIDRFVSTFLQQVEDGSWETGGWPQSLTDYSVSKLAVNAYTRLMAKIFT 274
           +LS+ + L+EE ID+ ++  L   ++G+ +   W + ++ Y VSK ++N YTR++AK   
Sbjct: 185 ILSDAENLTEERIDQVINQLLNDFKEGTVKEKNWAKFMSAYVVSKASLNGYTRVLAK--- 244

Query: 275 DRPEGHKIYVNCYCPGWVKTPMTGFAGNVSAEEGADTGVWLTLLPDQAVTGKFFAERREI 332
             PE     VN  CPG+VKT M    G +S EEGA + V L LLP Q      F  R+++
Sbjct: 245 KHPE---FRVNAVCPGFVKTDMNFKTGVLSVEEGASSPVRLALLPHQETPSGCFFSRKQV 294

BLAST of MS001786 vs. ExPASy Swiss-Prot
Match: B2X050 ((+)-neomenthol dehydrogenase OS=Capsicum annuum OX=4072 GN=MNR1 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.3e-44
Identity = 120/315 (38.10%), Postives = 174/315 (55.24%), Query Frame = 0

Query: 39  AVVTGGNRGIGFEISRQFAMHGLTVILTSRDVSVGLEAAKVLQEGGLNVD----FHQLDV 98
           AVVTGGN+GIG+E  RQ A  G+ V+LTSRD   G+EA + L+E     D    FHQLD+
Sbjct: 11  AVVTGGNKGIGYETCRQLASKGVVVVLTSRDEKKGIEAIERLKEESNFTDEHILFHQLDI 70

Query: 99  LDALSIKQFADWLLQNYGGLDILAN---------FGDI-IFK--CPNYVQIFLQINNAG- 158
           +D  SI    + +   +G LDIL N          GD+ + K     Y+ I    +  G 
Sbjct: 71  MDPASISSLVNLIKTKFGRLDILINNAGISGVMVEGDVQVLKEILERYISIVFTEDENGE 130

Query: 159 ----VNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPSAAGARIVNVSSRLGKLN 218
                    GS  + E  +  I TNY+G K M +A IPL++ S +  RIVNV+S +GKL 
Sbjct: 131 EGGWTKSGPGSVTNYELTKECIETNYYGAKRMTEAFIPLLQLSNS-PRIVNVASSMGKL- 190

Query: 219 GRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWETGGWPQSLTDYSVSKLA 278
                L N    E+L + D+L+EE +D+ V+ FL+   + S E+ GWP   T Y VSK +
Sbjct: 191 ---KLLCNKWAIEVLRDADSLTEEKVDQVVNEFLKDFTEKSTESKGWPSYFTAYKVSKAS 250

Query: 279 VNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVSAEEGADTGVWLTLLPDQ 333
           + AYTR++A  + +        +N  CPG+ KT +    G+++A EGA++ V L LLP+ 
Sbjct: 251 LIAYTRVLATKYPN------FRINSVCPGYCKTDVNANTGSLTAGEGAESLVNLALLPND 310

BLAST of MS001786 vs. ExPASy Swiss-Prot
Match: A4UHT7 (Salutaridine reductase OS=Papaver bracteatum OX=215227 GN=SALR PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.3e-43
Identity = 116/307 (37.79%), Postives = 166/307 (54.07%), Query Frame = 0

Query: 39  AVVTGGNRGIGFEISRQFAMHGLTVILTSRDVSVGLEAAKVLQEGG-LNVDFHQLDVLDA 98
           AVVTGGN+GIGFEI +Q +  G+ V+LT RDV+ GLEA + L+     NV FHQLDV D 
Sbjct: 15  AVVTGGNKGIGFEICKQLSSSGIMVVLTCRDVTRGLEAVEKLKNSNHENVVFHQLDVTDP 74

Query: 99  L-SIKQFADWLLQNYGGLDILANFGDIIFKCPNYVQIFLQINNAGVNFNLG--------- 158
           + ++   AD++   +G LDIL N   +     +  +    I++ G +             
Sbjct: 75  ITTMSSLADFIKARFGKLDILVNNAGVAGFSVDADRFKAMISDIGEDSEEVVKIYEKPEA 134

Query: 159 ---SSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPSAAGARIVNVSSRLGKLNGRRNRLE 218
               S + E A+  +  NY+G K++ + ++PL++ S +  RIVNVSS  G L      + 
Sbjct: 135 QELMSETYELAEECLKINYYGVKSVTEVLLPLLQLSDS-PRIVNVSSSTGSL----KYVS 194

Query: 219 NVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWETGGWPQSLTDYSVSKLAVNAYTRL 278
           N    E+L + D L+EE ID  V+  L+  ++   ET GWP     Y+ SK  +NAYTR+
Sbjct: 195 NETALEILGDGDALTEERIDMVVNMLLKDFKENLIETNGWPSFGAAYTTSKACLNAYTRV 254

Query: 279 MAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVSAEEGADTGVWLTLLPDQAVTGKFF 332
           +AK         K  VNC CPG VKT M    GN +A+EGA   V + L PD   +G FF
Sbjct: 255 LAKKIP------KFQVNCVCPGLVKTEMNYGIGNYTADEGAKHVVRIALFPDDGPSG-FF 309

BLAST of MS001786 vs. ExPASy Swiss-Prot
Match: Q071N0 (Salutaridine reductase OS=Papaver somniferum OX=3469 GN=SALR PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 3.5e-42
Identity = 116/307 (37.79%), Postives = 166/307 (54.07%), Query Frame = 0

Query: 39  AVVTGGNRGIGFEISRQFAMHGLTVILTSRDVSVGLEAAKVLQEGG-LNVDFHQLDVLDA 98
           AVVTGGN+GIGFEI +Q + +G+ V+LT RDV+ G EA + L+     NV FHQLDV D 
Sbjct: 15  AVVTGGNKGIGFEICKQLSSNGIMVVLTCRDVTKGHEAVEKLKNSNHENVVFHQLDVTDP 74

Query: 99  L-SIKQFADWLLQNYGGLDILANFGDIIFKCPNYVQIFLQINNAGVN------------F 158
           + ++   AD++  ++G LDIL N   +     +  +    I++ G +             
Sbjct: 75  IATMSSLADFIKTHFGKLDILVNNAGVAGFSVDADRFKAMISDIGEDSEELVKIYEKPEA 134

Query: 159 NLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPSAAGARIVNVSSRLGKLNGRRNRLE 218
               S + E A+  +  NY G K++ + +IPL++ S +  RIVNVSS  G L      + 
Sbjct: 135 QELMSETYELAEECLKINYNGVKSVTEVLIPLLQLSDS-PRIVNVSSSTGSL----KYVS 194

Query: 219 NVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWETGGWPQSLTDYSVSKLAVNAYTRL 278
           N    E+L + D L+EE ID  V+  L+  ++   ET GWP     Y+ SK  +NAYTR+
Sbjct: 195 NETALEILGDGDALTEERIDMVVNMLLKDFKENLIETNGWPSFGAAYTTSKACLNAYTRV 254

Query: 279 MAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVSAEEGADTGVWLTLLPDQAVTGKFF 332
           +A          K  VNC CPG VKT M    GN +AEEGA+  V + L PD   +G FF
Sbjct: 255 LANKIP------KFQVNCVCPGLVKTEMNYGIGNYTAEEGAEHVVRIALFPDDGPSG-FF 309

BLAST of MS001786 vs. ExPASy TrEMBL
Match: A0A6J1C0J7 (carbonyl reductase [NADPH] 1-like OS=Momordica charantia OX=3673 GN=LOC111007240 PE=3 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 2.8e-172
Identity = 313/332 (94.28%), Postives = 313/332 (94.28%), Query Frame = 0

Query: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60
           MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG
Sbjct: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60

Query: 61  LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDILANF 120
           LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDIL   
Sbjct: 61  LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDIL--- 120

Query: 121 GDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS 180
                           INNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS
Sbjct: 121 ----------------INNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS 180

Query: 181 AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE 240
           AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE
Sbjct: 181 AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE 240

Query: 241 TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS 300
           TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS
Sbjct: 241 TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS 300

Query: 301 AEEGADTGVWLTLLPDQAVTGKFFAERREISF 333
           AEEGADTGVWLTLLPDQAVTGKFFAERREISF
Sbjct: 301 AEEGADTGVWLTLLPDQAVTGKFFAERREISF 313

BLAST of MS001786 vs. ExPASy TrEMBL
Match: E5GBL1 (Short-chain dehydrogenase/reductase family protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.2e-162
Identity = 293/322 (90.99%), Postives = 303/322 (94.10%), Query Frame = 0

Query: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60
           MG KERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG
Sbjct: 1   MGKKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60

Query: 61  LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDILANF 120
           +TVILTSRDV VGLEAAKVLQEGGLNV FHQLDVLDALSIKQFADWLLQNYGGLDILAN 
Sbjct: 61  MTVILTSRDVCVGLEAAKVLQEGGLNVAFHQLDVLDALSIKQFADWLLQNYGGLDILAN- 120

Query: 121 GDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS 180
            DI  K P YVQ+  QINNAGVNFNLGSSNSVEFAQMVI+TNY+GTKNMIQAMIPLMKPS
Sbjct: 121 -DITSKFPIYVQLCPQINNAGVNFNLGSSNSVEFAQMVIATNYYGTKNMIQAMIPLMKPS 180

Query: 181 AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE 240
           +AGARIVNVSSRLGKLNGRRNR+ENV  RELLSNLDTL+EEVIDR VSTFLQQVEDGSWE
Sbjct: 181 SAGARIVNVSSRLGKLNGRRNRVENVEFRELLSNLDTLTEEVIDRIVSTFLQQVEDGSWE 240

Query: 241 TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS 300
           TGGWPQ  TDYSVSKLAVNAYTRLMAK FT+RPEGHKIYVNCYCPGWVKT MTGFAGN+S
Sbjct: 241 TGGWPQLSTDYSVSKLAVNAYTRLMAKKFTERPEGHKIYVNCYCPGWVKTAMTGFAGNIS 300

Query: 301 AEEGADTGVWLTLLPDQAVTGK 323
           AEEGADTGVWL LLPDQAVTG+
Sbjct: 301 AEEGADTGVWLALLPDQAVTGR 320

BLAST of MS001786 vs. ExPASy TrEMBL
Match: A0A1S3CLA0 (LOW QUALITY PROTEIN: carbonyl reductase [NADPH] 1-like OS=Cucumis melo OX=3656 GN=LOC103502267 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 2.9e-161
Identity = 293/332 (88.25%), Postives = 301/332 (90.66%), Query Frame = 0

Query: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60
           MG KERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG
Sbjct: 1   MGKKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60

Query: 61  LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDILANF 120
           +TVILTSRDV VGLEAAKVLQEGGLNV FHQLDVLDALSIKQFADWLLQNYGGLDIL   
Sbjct: 61  MTVILTSRDVCVGLEAAKVLQEGGLNVAFHQLDVLDALSIKQFADWLLQNYGGLDIL--- 120

Query: 121 GDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS 180
                           INNAGVNFNLGSSNSVEFAQMVI+TNY+GTKNMIQAMIPLMKPS
Sbjct: 121 ----------------INNAGVNFNLGSSNSVEFAQMVIATNYYGTKNMIQAMIPLMKPS 180

Query: 181 AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE 240
           +AGARIVNVSSRLGKLNGRRNR+ENV  RELLSNLDTL+EEVIDR VSTFLQQVEDGSWE
Sbjct: 181 SAGARIVNVSSRLGKLNGRRNRVENVEFRELLSNLDTLTEEVIDRIVSTFLQQVEDGSWE 240

Query: 241 TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS 300
           TGGWPQ  TDYSVSKLAVNAYTRLMAK  T+RPEGHKIYVNCYCPGWVKT MTGFAGN+S
Sbjct: 241 TGGWPQLSTDYSVSKLAVNAYTRLMAKKITERPEGHKIYVNCYCPGWVKTAMTGFAGNIS 300

Query: 301 AEEGADTGVWLTLLPDQAVTGKFFAERREISF 333
           AEEGADTGVWL LLPDQAVTGKFFAERREISF
Sbjct: 301 AEEGADTGVWLALLPDQAVTGKFFAERREISF 313

BLAST of MS001786 vs. ExPASy TrEMBL
Match: A0A0A0LNP6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G000030 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 5.0e-161
Identity = 293/332 (88.25%), Postives = 301/332 (90.66%), Query Frame = 0

Query: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60
           MG KERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG
Sbjct: 1   MGKKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60

Query: 61  LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDILANF 120
           +TVILTSRDV VGLEAAKVLQEGGLNV FHQLDVLDALSIKQFADWLLQNYGGLDIL   
Sbjct: 61  MTVILTSRDVCVGLEAAKVLQEGGLNVAFHQLDVLDALSIKQFADWLLQNYGGLDIL--- 120

Query: 121 GDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS 180
                           INNAGVNFNLGSSNSVEFAQMVI+TNY+GTKNMIQAMIPLMKPS
Sbjct: 121 ----------------INNAGVNFNLGSSNSVEFAQMVIATNYYGTKNMIQAMIPLMKPS 180

Query: 181 AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE 240
           +AGARIVNVSSRLGKLNGRRNR+ENV  RELLSNLDTL+EEVIDR VSTFLQQVEDGSWE
Sbjct: 181 SAGARIVNVSSRLGKLNGRRNRVENVEFRELLSNLDTLTEEVIDRIVSTFLQQVEDGSWE 240

Query: 241 TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS 300
           TGGWPQ  TDYSVSKLAVNAYTRLMAK FT+RPEGHKIYVNCYCPGWVKT MTGFAGN+S
Sbjct: 241 TGGWPQLSTDYSVSKLAVNAYTRLMAKKFTERPEGHKIYVNCYCPGWVKTAMTGFAGNIS 300

Query: 301 AEEGADTGVWLTLLPDQAVTGKFFAERREISF 333
           AEEGADTGVWL LLPDQAVTGK FAERREISF
Sbjct: 301 AEEGADTGVWLALLPDQAVTGKCFAERREISF 313

BLAST of MS001786 vs. ExPASy TrEMBL
Match: A0A6J1JJ29 (carbonyl reductase [NADPH] 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485563 PE=3 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.4e-157
Identity = 284/332 (85.54%), Postives = 295/332 (88.86%), Query Frame = 0

Query: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60
           MG KER  E REKRMQEISL RTIPYSDHQRWWSTETIAVVTGGNRGIGFE+SRQFAMHG
Sbjct: 1   MGRKERGNELREKRMQEISLTRTIPYSDHQRWWSTETIAVVTGGNRGIGFEVSRQFAMHG 60

Query: 61  LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDILANF 120
           +TVILTSRDV VGLEAAKVLQEGGLNV FHQLDVLDALSIKQFADWLLQNYGGLDIL   
Sbjct: 61  MTVILTSRDVCVGLEAAKVLQEGGLNVAFHQLDVLDALSIKQFADWLLQNYGGLDIL--- 120

Query: 121 GDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS 180
                           INNAGVNFNLGSSNSVE+AQMVI+TNY+GTKNM QAM+PLMKPS
Sbjct: 121 ----------------INNAGVNFNLGSSNSVEYAQMVIATNYYGTKNMTQAMVPLMKPS 180

Query: 181 AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE 240
           +AGARIVNVSSRL KLNGRRNR+ENVA RELL NLDTL+EEVIDR VSTFL+QVEDGSWE
Sbjct: 181 SAGARIVNVSSRLAKLNGRRNRVENVAFRELLGNLDTLTEEVIDRIVSTFLEQVEDGSWE 240

Query: 241 TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS 300
           TGGWPQ  TDYSVSKLAVNAYTRLMAKI  DRPEGHKIYVNCYCPGWVKT MTGFAGN+S
Sbjct: 241 TGGWPQLSTDYSVSKLAVNAYTRLMAKILGDRPEGHKIYVNCYCPGWVKTAMTGFAGNIS 300

Query: 301 AEEGADTGVWLTLLPDQAVTGKFFAERREISF 333
           AEEGADTGVWL LLPDQAVTGKFFAERREISF
Sbjct: 301 AEEGADTGVWLALLPDQAVTGKFFAERREISF 313

BLAST of MS001786 vs. TAIR 10
Match: AT5G51030.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 473.4 bits (1217), Expect = 1.5e-133
Identity = 224/332 (67.47%), Postives = 279/332 (84.04%), Query Frame = 0

Query: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60
           +G+KE++KE+R+KR+QEISLLRTIPYSDHQRWW++ET+AVVTG NRGIGFE+ RQ A HG
Sbjct: 2   VGSKEKSKEKRDKRLQEISLLRTIPYSDHQRWWTSETVAVVTGANRGIGFEMVRQLAGHG 61

Query: 61  LTVILTSRDVSVGLEAAKVLQEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDILANF 120
           LTVILTSRD +VG+EAAK+LQEGG NVDFH+LD+LD+ SI++F +W+ + YG +D+L   
Sbjct: 62  LTVILTSRDENVGVEAAKILQEGGFNVDFHRLDILDSSSIQEFCEWIKEKYGFIDVL--- 121

Query: 121 GDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPS 180
                           INNAGVN+N+GS NSVEF+ MVISTNY+GTKN+I AMIPLM+ +
Sbjct: 122 ----------------INNAGVNYNVGSDNSVEFSHMVISTNYYGTKNIINAMIPLMRHA 181

Query: 181 AAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWE 240
             GARIVNV+SRLG+L GR ++LEN  VR  L ++D+L+EE++D+ VS FL+QVE+G+WE
Sbjct: 182 CQGARIVNVTSRLGRLKGRHSKLENEDVRAKLMDVDSLTEEIVDKTVSEFLKQVEEGTWE 241

Query: 241 TGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVS 300
           +GGWP S TDYSVSK+AVNAYTR++AK  ++RPEG KIY NC+CPGWVKT MTG+AGNVS
Sbjct: 242 SGGWPHSFTDYSVSKMAVNAYTRVLAKELSERPEGEKIYANCFCPGWVKTAMTGYAGNVS 301

Query: 301 AEEGADTGVWLTLLPDQAVTGKFFAERREISF 333
           AE+GADTGVWL LLPDQA+TGKFFAERREISF
Sbjct: 302 AEDGADTGVWLALLPDQAITGKFFAERREISF 314

BLAST of MS001786 vs. TAIR 10
Match: AT5G61830.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 442.2 bits (1136), Expect = 3.7e-124
Identity = 225/335 (67.16%), Postives = 266/335 (79.40%), Query Frame = 0

Query: 1   MGTKERAKERREKRMQEISLLRTIPYSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHG 60
           M  KE+A+ERREKRMQEISLLRTIPYSDH RWWS E +AVVTG NRGIGFEI+RQ A+HG
Sbjct: 1   MTNKEKARERREKRMQEISLLRTIPYSDHHRWWSCENVAVVTGSNRGIGFEIARQLAVHG 60

Query: 61  LTVILTSRDVSVGLEAAKVL--QEGGLNVDFHQLDVLDALSIKQFADWLLQNYGGLDILA 120
           LTV+LT+R+V+ GLEA K L  QE GL V FHQLDV D+ SI++F  WL Q +GGLDIL 
Sbjct: 61  LTVVLTARNVNAGLEAVKSLRHQEEGLKVYFHQLDVTDSSSIREFGCWLKQTFGGLDIL- 120

Query: 121 NFGDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMK 180
                             +NNAGVN+NLGS N+VEFA+ VISTNY GTKNM +AMIPLM+
Sbjct: 121 ------------------VNNAGVNYNLGSDNTVEFAETVISTNYQGTKNMTKAMIPLMR 180

Query: 181 PSAAGARIVNVSSRLGKLNGRRNRLENVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGS 240
           PS  GAR+VNVSSRLG++NGRRNRL NV +R+ LS+ D L+EE+IDR VS F+ QV+DG+
Sbjct: 181 PSPHGARVVNVSSRLGRVNGRRNRLANVELRDQLSSPDLLTEELIDRTVSKFINQVKDGT 240

Query: 241 WETGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGN 300
           WE+GGWPQ+ TDYS+SKLAVNAYTRLMAK    R E  KIYVN +CPGWVKT MTG+AGN
Sbjct: 241 WESGGWPQTFTDYSMSKLAVNAYTRLMAKELERRGEEEKIYVNSFCPGWVKTAMTGYAGN 300

Query: 301 VSAEEGADTGVWLTL-LPDQAVTGKFFAERREISF 333
           +  E+ ADTGVWL+L L ++AVTGKFFAERREI+F
Sbjct: 301 MPPEDAADTGVWLSLVLSEEAVTGKFFAERREINF 316

BLAST of MS001786 vs. TAIR 10
Match: AT3G59710.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 222.2 bits (565), Expect = 6.0e-58
Identity = 125/301 (41.53%), Postives = 183/301 (60.80%), Query Frame = 0

Query: 26  YSDHQRWWSTETIAVVTGGNRGIGFEISRQFAMHGLTVILTSRDVSVGLEAAKVLQEGGL 85
           Y     WWS ET AVVTG N+GIGF + ++    GLTV+LT+R+   G +AA+ L+  G 
Sbjct: 18  YGIIHEWWSEETTAVVTGANKGIGFAVVKRLLELGLTVVLTARNAENGSQAAESLRRIGF 77

Query: 86  -NVDFHQLDVLDALSIKQFADWLLQNYGGLDILANFGDIIFKCPNYVQIFLQINNAGVNF 145
            NV F  LD+ D  SI  FA W  +N G LDIL                   +NNA V+F
Sbjct: 78  GNVHFCCLDISDPSSIAAFASWFGRNLGILDIL-------------------VNNAAVSF 137

Query: 146 NLGSSNSVEFAQMVISTNYFGTKNMIQAMIPLMKPSAAGARIVNVSSRLGKLNGRRNRLE 205
           N    N ++  + +I TN++G K + +A++PL + S + +RI+N+SSRLG L    N+L 
Sbjct: 138 NAVGENLIKEPETIIKTNFYGAKLLTEALLPLFRRSVSVSRILNMSSRLGTL----NKLR 197

Query: 206 NVAVRELLSNLDTLSEEVIDRFVSTFLQQVEDGSWETGGWPQSLTDYSVSKLAVNAYTRL 265
           + ++R +L + D L+ E ID  ++ FLQ V+ G+WE  GWP++  DY++SKLA+NAY+R+
Sbjct: 198 SPSIRRILESED-LTNEQIDATLTQFLQDVKSGTWEKQGWPENWPDYAISKLALNAYSRV 257

Query: 266 MAKIFTDRPEGHKIYVNCYCPGWVKTPMTGFAGNVSAEEGADTGVWLTLL-PDQAVTGKF 325
           +A+    R +G K+ VNC CPG+ +T MTG  G  +A+E A     L LL P++  TGKF
Sbjct: 258 LAR----RYDGKKLSVNCLCPGFTRTSMTGGQGTHTADEAAAIVAKLVLLPPEKLATGKF 290

BLAST of MS001786 vs. TAIR 10
Match: AT2G24190.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 183.3 bits (464), Expect = 3.1e-46
Identity = 114/297 (38.38%), Postives = 170/297 (57.24%), Query Frame = 0

Query: 39  AVVTGGNRGIGFEISRQFAMHGLTVILTSRDVSVGLEAAKVLQE----GGLNVDFHQLDV 98
           A+VTGGNRGIGFEI RQ A  G+ VILTSRD   GLEA + L++       ++ FHQLDV
Sbjct: 9   AIVTGGNRGIGFEICRQLANKGIRVILTSRDEKQGLEAVETLKKELEISDQSIVFHQLDV 68

Query: 99  LDALSIKQFADWLLQNYGGLDILANFGDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEF 158
            D +S+   A+++  ++G LDIL N   +     +   +       G  +    + + E 
Sbjct: 69  SDPVSVTSLAEFVKTHFGKLDILINNAGVGGVITDVDALRAGTGKEGFKWEETITETYEL 128

Query: 159 AQMVISTNYFGTKNMIQAMIPLMKPSAAGARIVNVSSRLGKLNGRRNRLENVAVRELLSN 218
           A+  I  NY+G K M +A IPL++ S +  RI+NVSS +G++      L N   + +LS+
Sbjct: 129 AEECIKINYYGPKRMCEAFIPLLQLSDS-PRIINVSSFMGQV----KNLVNEWAKGILSD 188

Query: 219 LDTLSEEVIDRFVSTFLQQVEDGSWETGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPE 278
            + L+E  ID+ ++  L  +++ + +T  W + ++ Y VSK  +NAYTR++AK     PE
Sbjct: 189 AENLTEVRIDQVINQLLNDLKEDTAKTKYWAKVMSAYVVSKAGLNAYTRILAK---KHPE 248

Query: 279 GHKIYVNCYCPGWVKTPMTGFAGNVSAEEGADTGVWLTLLPDQAVTGKFFAERREIS 332
              I VN  CPG+VKT M    G +S EEGA + V L LLP Q      F +R+++S
Sbjct: 249 ---IRVNSVCPGFVKTDMNFKTGILSVEEGASSPVRLALLPHQESPSGCFFDRKQVS 294

BLAST of MS001786 vs. TAIR 10
Match: AT2G24190.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 183.3 bits (464), Expect = 3.1e-46
Identity = 114/297 (38.38%), Postives = 170/297 (57.24%), Query Frame = 0

Query: 39  AVVTGGNRGIGFEISRQFAMHGLTVILTSRDVSVGLEAAKVLQE----GGLNVDFHQLDV 98
           A+VTGGNRGIGFEI RQ A  G+ VILTSRD   GLEA + L++       ++ FHQLDV
Sbjct: 14  AIVTGGNRGIGFEICRQLANKGIRVILTSRDEKQGLEAVETLKKELEISDQSIVFHQLDV 73

Query: 99  LDALSIKQFADWLLQNYGGLDILANFGDIIFKCPNYVQIFLQINNAGVNFNLGSSNSVEF 158
            D +S+   A+++  ++G LDIL N   +     +   +       G  +    + + E 
Sbjct: 74  SDPVSVTSLAEFVKTHFGKLDILINNAGVGGVITDVDALRAGTGKEGFKWEETITETYEL 133

Query: 159 AQMVISTNYFGTKNMIQAMIPLMKPSAAGARIVNVSSRLGKLNGRRNRLENVAVRELLSN 218
           A+  I  NY+G K M +A IPL++ S +  RI+NVSS +G++      L N   + +LS+
Sbjct: 134 AEECIKINYYGPKRMCEAFIPLLQLSDS-PRIINVSSFMGQV----KNLVNEWAKGILSD 193

Query: 219 LDTLSEEVIDRFVSTFLQQVEDGSWETGGWPQSLTDYSVSKLAVNAYTRLMAKIFTDRPE 278
            + L+E  ID+ ++  L  +++ + +T  W + ++ Y VSK  +NAYTR++AK     PE
Sbjct: 194 AENLTEVRIDQVINQLLNDLKEDTAKTKYWAKVMSAYVVSKAGLNAYTRILAK---KHPE 253

Query: 279 GHKIYVNCYCPGWVKTPMTGFAGNVSAEEGADTGVWLTLLPDQAVTGKFFAERREIS 332
              I VN  CPG+VKT M    G +S EEGA + V L LLP Q      F +R+++S
Sbjct: 254 ---IRVNSVCPGFVKTDMNFKTGILSVEEGASSPVRLALLPHQESPSGCFFDRKQVS 299

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135225.15.8e-17294.28carbonyl reductase [NADPH] 1-like [Momordica charantia][more]
KAG7023020.12.4e-16589.16Salutaridine reductase [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG7021365.13.1e-16589.55Short-chain dehydrogenase/reductase 2b, partial [Cucurbita argyrosperma subsp. a... [more]
XP_038880046.15.0e-16389.16carbonyl reductase [NADPH] 1-like [Benincasa hispida][more]
ADN33869.12.5e-16290.99short-chain dehydrogenase/reductase family protein [Cucumis melo subsp. melo][more]
Match NameE-valueIdentityDescription
Q9ZUH54.4e-4538.38Short-chain dehydrogenase/reductase 2b OS=Arabidopsis thaliana OX=3702 GN=SDR2b ... [more]
Q9M2E27.4e-4538.54(+)-neomenthol dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=SDR1 PE=1 SV=1[more]
B2X0501.3e-4438.10(+)-neomenthol dehydrogenase OS=Capsicum annuum OX=4072 GN=MNR1 PE=1 SV=1[more]
A4UHT75.3e-4337.79Salutaridine reductase OS=Papaver bracteatum OX=215227 GN=SALR PE=1 SV=1[more]
Q071N03.5e-4237.79Salutaridine reductase OS=Papaver somniferum OX=3469 GN=SALR PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1C0J72.8e-17294.28carbonyl reductase [NADPH] 1-like OS=Momordica charantia OX=3673 GN=LOC111007240... [more]
E5GBL11.2e-16290.99Short-chain dehydrogenase/reductase family protein OS=Cucumis melo subsp. melo O... [more]
A0A1S3CLA02.9e-16188.25LOW QUALITY PROTEIN: carbonyl reductase [NADPH] 1-like OS=Cucumis melo OX=3656 G... [more]
A0A0A0LNP65.0e-16188.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G000030 PE=3 SV=1[more]
A0A6J1JJ293.4e-15785.54carbonyl reductase [NADPH] 1-like OS=Cucurbita maxima OX=3661 GN=LOC111485563 PE... [more]
Match NameE-valueIdentityDescription
AT5G51030.11.5e-13367.47NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G61830.13.7e-12467.16NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G59710.16.0e-5841.53NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G24190.13.1e-4638.38NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G24190.23.1e-4638.38NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 251..270
score: 29.67
coord: 112..123
score: 38.71
coord: 38..55
score: 43.74
coord: 276..293
score: 39.14
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 251..270
score: 32.19
coord: 131..142
score: 38.33
coord: 184..192
score: 44.04
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 38..196
e-value: 3.0E-24
score: 85.6
NoneNo IPR availableGENE3D3.40.50.720coord: 30..332
e-value: 4.6E-76
score: 257.6
NoneNo IPR availablePANTHERPTHR43490(+)-NEOMENTHOL DEHYDROGENASEcoord: 129..332
NoneNo IPR availablePANTHERPTHR43490:SF89(+)-NEOMENTHOL DEHYDROGENASE-RELATEDcoord: 129..332
NoneNo IPR availablePANTHERPTHR43490:SF89(+)-NEOMENTHOL DEHYDROGENASE-RELATEDcoord: 25..125
NoneNo IPR availablePANTHERPTHR43490(+)-NEOMENTHOL DEHYDROGENASEcoord: 25..125
NoneNo IPR availableCDDcd05324carb_red_PTCR-like_SDR_ccoord: 38..332
e-value: 1.4699E-90
score: 268.336
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 238..266
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 38..324

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS001786.1MS001786.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity