MS001321 (gene) Bitter gourd (TR) v1

BLAST of MS001321 vs. NCBI nr
Match: XP_022131760.1 (uncharacterized protein LOC111004847 [Momordica charantia])

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 576/582 (98.97%), Postives = 579/582 (99.48%), Query Frame = 0

Query: 1   MAKMKKFQVKLQKLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR 60
           MAKMKKFQVKL+KLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR
Sbjct: 1   MAKMKKFQVKLEKLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR 60

Query: 61  FLNNGQETVEWDDEFHSICEFSAADDASIGSWDTNFYVLLENSTKSKTKSSVLGKASLNL 120
           FLNNGQETVEWDDEFHSICEFSAADDASIGSWDTNFYVLLENSTKSKTKSSVLGKASLNL
Sbjct: 61  FLNNGQETVEWDDEFHSICEFSAADDASIGSWDTNFYVLLENSTKSKTKSSVLGKASLNL 120

Query: 121 AEVLSEMEAKIDTILPITLKDAAGPRPATLSVSVRVNFVEVRDDPDPTRIARDPAQDEKD 180
           AEVLSEMEAKID ILPITLKDAAGPRPATL  SVRVNFVEVRDDPDPTRIARDPAQDEKD
Sbjct: 121 AEVLSEMEAKIDAILPITLKDAAGPRPATL--SVRVNFVEVRDDPDPTRIARDPAQDEKD 180

Query: 181 GFFRALKDLTSFKKKNRDKAISSDGENAVVGNRSEEEGDQKQGKLSWKKRRLSFSFRNSK 240
           GFFRALKDLTSFKKKNRDKAISSDGENAVVGNRSEEEGDQKQGKLSWKKRRLSFSFRNSK
Sbjct: 181 GFFRALKDLTSFKKKNRDKAISSDGENAVVGNRSEEEGDQKQGKLSWKKRRLSFSFRNSK 240

Query: 241 GKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTGVKTADQVSLELDGQNREGT 300
           GKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTGVKTADQVSLELDGQNREGT
Sbjct: 241 GKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTGVKTADQVSLELDGQNREGT 300

Query: 301 GGRWETREIVSRDGKAKLKTELFFGSFDQRSEKASGESACTAIVAVITHWLHSNQGAMPT 360
           GGRWETREIVSRDGKAKLKTELFFGSFDQRSEKA+GESACTAIVAVITHWLHSNQGAMPT
Sbjct: 301 GGRWETREIVSRDGKAKLKTELFFGSFDQRSEKANGESACTAIVAVITHWLHSNQGAMPT 360

Query: 361 RPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLPENSFVGFFSP 420
           RPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLPENSFVGFFSP
Sbjct: 361 RPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLPENSFVGFFSP 420

Query: 421 EKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVLEMEEDACYIVDSLG 480
           EKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVL+MEEDACYIVDSLG
Sbjct: 421 EKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVLKMEEDACYIVDSLG 480

Query: 481 ERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLAALTIEELEQE 540
           ERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLAALTIEELEQE
Sbjct: 481 ERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLAALTIEELEQE 540

Query: 541 QRKLSCNFIPHQRLQIDFHFSSPVDSSSSTSPFSVFFDEDSA 583
           QRKLSCNFIPHQRLQIDFHFSSPVDSSSSTSPFSVFFDEDSA
Sbjct: 541 QRKLSCNFIPHQRLQIDFHFSSPVDSSSSTSPFSVFFDEDSA 580

BLAST of MS001321 vs. NCBI nr
Match: KAG7029507.1 (hypothetical protein SDJN02_07846, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 818.1 bits (2112), Expect = 4.7e-233
Identity = 434/586 (74.06%), Postives = 493/586 (84.13%), Query Frame = 0

Query: 1   MAKMKKFQVKLQKLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR 60
           MAKMKKFQVKL  LKL+GFG E EA+AIEIKWKGP +HS+LSVPFYGKS LQ +RT  QR
Sbjct: 1   MAKMKKFQVKLGDLKLHGFGNEKEAIAIEIKWKGPHRHSILSVPFYGKSPLQTDRTTAQR 60

Query: 61  FLNNGQETVEWDDEFHSICEFSAADD-ASIGSWDTNFYVLLENSTKSKTKSSVLGKASLN 120
            L   Q+T++WD EF SICEF  ADD +SI SWDT FYVLLE STKSKTK SVLGKASLN
Sbjct: 61  ALIQTQQTLQWDHEFLSICEFEFADDSSSIASWDTKFYVLLEESTKSKTKFSVLGKASLN 120

Query: 121 LAEVLSEMEAKIDTILPITLKDAAGPRPATLSVSVRVNFVEVRDDPDPTRIARDPAQDEK 180
           LAE+L EME  ++  +PITLK A+    ATL  SVRVNFVEVRDD DP +      Q +K
Sbjct: 121 LAEMLLEMETIVERNVPITLKGAS--HQATL--SVRVNFVEVRDDSDPIQ-----QQQDK 180

Query: 181 DGFFRALKDLTSFKKKNRD--KAISSDGENAVVGNRSEEE-GDQKQ-GKLSWKKRRLSFS 240
           +GF +ALKDLTSF+KKNRD  KA+SSDG+N  + +  EE+ GDQKQ GKL  KKRRLSFS
Sbjct: 181 EGFLKALKDLTSFRKKNRDKGKAVSSDGQNRGLADPKEEDGGDQKQLGKLLSKKRRLSFS 240

Query: 241 FRNSKGKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTGVKTADQVSLELDGQ 300
           FR SKGKVEPWSEKT+TAVND V+VDRQQ D + +  P++  SQT    + ++SLE+D Q
Sbjct: 241 FRYSKGKVEPWSEKTNTAVNDGVSVDRQQHDTEPTA-PISTTSQTEKGESTEISLEIDEQ 300

Query: 301 NREGTGGRWETREIVSRDGKAKLKTELFFGSFDQRSEKASGESACTAIVAVITHWLHSNQ 360
           N+E + GRWET+EIVSRDGKAKLKTE+FFGSFDQRSEKA GESACTAIVAVITHWLHSN 
Sbjct: 301 NKEDSRGRWETKEIVSRDGKAKLKTEVFFGSFDQRSEKAGGESACTAIVAVITHWLHSNY 360

Query: 361 GAMPTRPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLPENSFV 420
           GAMPTRPELD+L+MEGSSEWQKLC+N CYS+SFPNKHFDLETV+EA+VRPITV  ENSFV
Sbjct: 361 GAMPTRPELDNLIMEGSSEWQKLCNNHCYSNSFPNKHFDLETVMEADVRPITVSAENSFV 420

Query: 421 GFFSPEKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVLEMEEDACYI 480
           GFFSPEKFNCL+EAMSFEQIW EV N  KT    YE R+YIVSWNDHFFVL+MEE+ACYI
Sbjct: 421 GFFSPEKFNCLSEAMSFEQIWNEV-NTTKTCPN-YETRVYIVSWNDHFFVLKMEENACYI 480

Query: 481 VDSLGERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLAALTIE 540
           VDSLGERLFEGCNQAYILKFDSSSLM+E+KE+GE+ ELVC+GKECCREFF+RFLAA+TIE
Sbjct: 481 VDSLGERLFEGCNQAYILKFDSSSLMYESKEKGEVGELVCKGKECCREFFERFLAAITIE 540

Query: 541 ELEQEQRK-LSCNFIPHQRLQIDFHFSSPVDSSSSTSPFSVFFDED 581
           ELE+EQ+K  SCNFIPHQRLQIDFHFSSPV SS STSP S+F D+D
Sbjct: 541 ELEEEQKKQSSCNFIPHQRLQIDFHFSSPVVSSLSTSPCSIFSDQD 574

BLAST of MS001321 vs. NCBI nr
Match: XP_022996565.1 (uncharacterized protein LOC111491769 [Cucurbita maxima])

HSP 1 Score: 818.1 bits (2112), Expect = 4.7e-233
Identity = 432/585 (73.85%), Postives = 492/585 (84.10%), Query Frame = 0

Query: 1   MAKMKKFQVKLQKLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR 60
           MAKMKKFQVKL  LKL+GFG E EA+AIEIKWKGPQ+HS+LSVPFYGKS LQ +RT  QR
Sbjct: 1   MAKMKKFQVKLGDLKLHGFGNEKEAIAIEIKWKGPQRHSILSVPFYGKSPLQTDRTTAQR 60

Query: 61  FLNNGQETVEWDDEFHSICEFSAADDASIGSWDTNFYVLLENSTKSKTKSSVLGKASLNL 120
            L    +T++WD EF SICEF  ADD+S  SWDT FYVLLE STKSKTK SVLGKASLNL
Sbjct: 61  PL---IQTLQWDHEFLSICEFEFADDSSTASWDTKFYVLLEESTKSKTKFSVLGKASLNL 120

Query: 121 AEVLSEMEAKIDTILPITLKDAAGPRPATLSVSVRVNFVEVRDDPDPTRIARDPAQDEKD 180
           A++L EME K++  +PI LKDA    P  +++SVRVNFVEVRDD D  +      Q +K+
Sbjct: 121 AKMLLEMETKVERNVPIMLKDA----PHQVTLSVRVNFVEVRDDSDTIQ------QQDKE 180

Query: 181 GFFRALKDLTSFKKKNRD--KAISSDGENAVVGNRSEEE-GDQKQ-GKLSWKKRRLSFSF 240
           GF +ALKDLTSF+KKNRD  KAISSDG+N  +G+  EE+ GDQKQ GKL  KKRRLSFSF
Sbjct: 181 GFLKALKDLTSFRKKNRDKGKAISSDGQNRALGDPKEEDGGDQKQLGKLLSKKRRLSFSF 240

Query: 241 RNSKGKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTGVKTADQVSLELDGQN 300
           R SKGKVEPWSEKT+TAVND V+VDRQQ D + +  P++  SQT    + ++SLE+D QN
Sbjct: 241 RYSKGKVEPWSEKTNTAVNDGVSVDRQQHDTEPTA-PISTTSQTEKGESTEISLEIDEQN 300

Query: 301 REGTGGRWETREIVSRDGKAKLKTELFFGSFDQRSEKASGESACTAIVAVITHWLHSNQG 360
           +E + GRWET+EIVSRDGKAKLKTE+FFGSFDQRSEKA GESACTAIVAVITHWLHSN G
Sbjct: 301 KEDSRGRWETKEIVSRDGKAKLKTEVFFGSFDQRSEKAGGESACTAIVAVITHWLHSNYG 360

Query: 361 AMPTRPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLPENSFVG 420
           AMPTRPELD+L+MEGSSEWQKLC+N CYS+SFPNKHFDLETV+EA+VRPITV  ENSFVG
Sbjct: 361 AMPTRPELDNLIMEGSSEWQKLCNNHCYSNSFPNKHFDLETVMEADVRPITVSAENSFVG 420

Query: 421 FFSPEKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVLEMEEDACYIV 480
           FFSPEKFNCL+EAMSFEQIW  V  N KT    YE R+YIVSWNDHFFVL+MEEDACYIV
Sbjct: 421 FFSPEKFNCLSEAMSFEQIWNLV--NTKTCPN-YETRVYIVSWNDHFFVLKMEEDACYIV 480

Query: 481 DSLGERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLAALTIEE 540
           DSLGERLFEGCNQAYILKFDSSSLM+E+KEEGE+ ELVC+GKECCREFF+RFLAA+TIEE
Sbjct: 481 DSLGERLFEGCNQAYILKFDSSSLMYESKEEGEVGELVCKGKECCREFFERFLAAITIEE 540

Query: 541 LEQEQRK-LSCNFIPHQRLQIDFHFSSPVDSSSSTSPFSVFFDED 581
           LE+EQ+K  SC+FIPHQRLQIDFHFSSPV SS STSP S+F D+D
Sbjct: 541 LEEEQKKQSSCSFIPHQRLQIDFHFSSPVVSSLSTSPCSIFSDQD 568

BLAST of MS001321 vs. NCBI nr
Match: KAG6598568.1 (hypothetical protein SDJN03_08346, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 817.0 bits (2109), Expect = 1.0e-232
Identity = 434/586 (74.06%), Postives = 492/586 (83.96%), Query Frame = 0

Query: 1   MAKMKKFQVKLQKLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR 60
           MAKMKKFQVKL  LKL+GFG E EA+AIEIKWKGP +HS+LSVPFYGKS LQ +RT  QR
Sbjct: 1   MAKMKKFQVKLGDLKLHGFGNEKEAIAIEIKWKGPHRHSILSVPFYGKSPLQTDRTIAQR 60

Query: 61  FLNNGQETVEWDDEFHSICEFSAADD-ASIGSWDTNFYVLLENSTKSKTKSSVLGKASLN 120
            L   Q+T++WD EF SICEF  ADD +SI SWDT FYVLLE STKSKTK SVLGKASLN
Sbjct: 61  ALIQTQQTLQWDHEFLSICEFEFADDSSSIASWDTKFYVLLEESTKSKTKVSVLGKASLN 120

Query: 121 LAEVLSEMEAKIDTILPITLKDAAGPRPATLSVSVRVNFVEVRDDPDPTRIARDPAQDEK 180
           LAE+L EME  ++  +PITLK A+    ATL  SVRVNFVEVRDD DP +      Q +K
Sbjct: 121 LAEMLLEMETIVERNVPITLKGAS--HQATL--SVRVNFVEVRDDSDPIQ-----QQQDK 180

Query: 181 DGFFRALKDLTSFKKKNRD--KAISSDGENAVVGNRSEEE-GDQKQ-GKLSWKKRRLSFS 240
           +GF +ALKDLTSF+KKNRD  KA+SSDG+N  + +  EE+ GDQKQ GKL  KKRRLSFS
Sbjct: 181 EGFLKALKDLTSFRKKNRDKGKAVSSDGQNRGLADPKEEDGGDQKQLGKLLSKKRRLSFS 240

Query: 241 FRNSKGKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTGVKTADQVSLELDGQ 300
           FR SKGKVEPWSEKT+TAVND V+VDRQQ D + +  P++  SQT    + ++SLE+D Q
Sbjct: 241 FRYSKGKVEPWSEKTNTAVNDGVSVDRQQHDTEPTA-PISTTSQTEKGESTEISLEIDEQ 300

Query: 301 NREGTGGRWETREIVSRDGKAKLKTELFFGSFDQRSEKASGESACTAIVAVITHWLHSNQ 360
           N+E + GRWET+EIVSRDGKAKLKTE+FFGSFDQRSEKA GESACTAIVAVITHWLHSN 
Sbjct: 301 NKEDSRGRWETKEIVSRDGKAKLKTEVFFGSFDQRSEKAGGESACTAIVAVITHWLHSNY 360

Query: 361 GAMPTRPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLPENSFV 420
            AMPTRPELD+L+MEGSSEWQKLC+N CYS+SFPNKHFDLETV+EA+VRPITV  ENSFV
Sbjct: 361 SAMPTRPELDNLIMEGSSEWQKLCNNHCYSNSFPNKHFDLETVMEADVRPITVSAENSFV 420

Query: 421 GFFSPEKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVLEMEEDACYI 480
           GFFSPEKFNCL+EAMSFEQIW EV N  KT    YE R+YIVSWNDHFFVL+MEEDACYI
Sbjct: 421 GFFSPEKFNCLSEAMSFEQIWNEV-NTTKTCPN-YETRVYIVSWNDHFFVLKMEEDACYI 480

Query: 481 VDSLGERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLAALTIE 540
           VDSLGERLFEGCNQAYILKFDSSSLM+E+KE+GE+ ELVC+GKECCREFF+RFLAA+TIE
Sbjct: 481 VDSLGERLFEGCNQAYILKFDSSSLMYESKEKGEVGELVCKGKECCREFFERFLAAITIE 540

Query: 541 ELEQEQRK-LSCNFIPHQRLQIDFHFSSPVDSSSSTSPFSVFFDED 581
           ELE+EQ+K  SCNFIPHQRLQIDFHFSSPV SS STSP S+F D+D
Sbjct: 541 ELEEEQKKQSSCNFIPHQRLQIDFHFSSPVVSSLSTSPCSIFSDQD 574

BLAST of MS001321 vs. NCBI nr
Match: XP_038885600.1 (uncharacterized protein LOC120075923 [Benincasa hispida])

HSP 1 Score: 817.0 bits (2109), Expect = 1.0e-232
Identity = 430/595 (72.27%), Postives = 491/595 (82.52%), Query Frame = 0

Query: 1   MAKMKKFQVKLQKLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR 60
           M KMKKFQVKL+ LKL G+GKENE +AIEIKWKGPQ+HSLL VPFY KS LQ NRT  QR
Sbjct: 1   MVKMKKFQVKLENLKLLGYGKENERIAIEIKWKGPQRHSLLPVPFYAKSPLQTNRTTAQR 60

Query: 61  FLNNGQETVEWDDEFHSICEFSAADD--ASIGSWDTNFYVLLENS-TKSKTKSSVLGKAS 120
            L+N  +TV+W+ EFHS+CEF    D  +SI  WDT FYVLLE   TKSKTKSSVLGKAS
Sbjct: 61  VLSNHHQTVQWNHEFHSMCEFEFPHDDASSIPFWDTKFYVLLEEEPTKSKTKSSVLGKAS 120

Query: 121 LNLAEVLSEMEAKIDTILPITLKDAAGPRPATLSVSVRVNFVEVRDDPDPTRIARDPAQD 180
           LNLAE+LS ME K++  +PI LKD+AG  P   ++SV VNFVEVRD PDP +      Q+
Sbjct: 121 LNLAEMLSAMETKMERNVPIVLKDSAGAAPHHATISVCVNFVEVRDGPDPIQ------QE 180

Query: 181 EKDGFFRALKDLTSFKKKNRD--KAISSDGENAVVGNRS--EEEGDQKQGKLSWKKRRLS 240
           +K+GF + LKDLTSFKKKNR+  K ISSDGEN  +G+ +  EE+GD KQGK   KKRRLS
Sbjct: 181 DKEGFLKTLKDLTSFKKKNREKGKVISSDGENRGLGDSTTGEEDGDHKQGKSLTKKRRLS 240

Query: 241 FSFRNSKGKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTG---VKTADQVSL 300
           FSFR+S+ KVEPW EKT+TAVND VTVDRQ+ D  +SVF VAPIS T       + + SL
Sbjct: 241 FSFRHSRRKVEPWLEKTNTAVNDGVTVDRQRHDNGASVFNVAPISTTSQMEKAESTEFSL 300

Query: 301 ELDGQNREGTGGRWETREIVSRDGKAKLKTELFFGSFDQRSEKASGESACTAIVAVITHW 360
           E + Q +E +GGRWETREIVSRDGK KLKTE+FFGSFDQRSEKA GESACTAIVAVI HW
Sbjct: 301 ETNIQYKEASGGRWETREIVSRDGKLKLKTEVFFGSFDQRSEKAGGESACTAIVAVIAHW 360

Query: 361 LHSNQGAMPTRPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLP 420
           LHSN G MPT+ ELD+L+MEGSSEWQKLC+N+CYS+SFPNKHFDLETVV+A+V PITV  
Sbjct: 361 LHSNYGVMPTQLELDNLIMEGSSEWQKLCNNECYSNSFPNKHFDLETVVQADVGPITVSA 420

Query: 421 ENSFVGFFSPEKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVLEMEE 480
           ENSFVGFFSPEKFNCLTEAMSFEQIW EVN    ++   YE RIYIVSWNDHFFVL+M+E
Sbjct: 421 ENSFVGFFSPEKFNCLTEAMSFEQIWNEVNTKTFSN---YESRIYIVSWNDHFFVLKMDE 480

Query: 481 DACYIVDSLGERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLA 540
           +ACYI+DSLGERLFEGCNQAYILKFDSSSLMFEN+E+GE+ ELVCRGKECCREFF+RFLA
Sbjct: 481 NACYIIDSLGERLFEGCNQAYILKFDSSSLMFENQEKGEVGELVCRGKECCREFFERFLA 540

Query: 541 ALTIEELEQEQRKL-SCNFIPHQRLQIDFHFSSPV--DSSSSTSPFSVFFDEDSA 583
           A+TIEELE+EQ+KL SCNFIPHQRLQIDFHFSSPV   SSSSTSP S+FFD+DSA
Sbjct: 541 AITIEELEEEQKKLGSCNFIPHQRLQIDFHFSSPVASSSSSSTSPSSLFFDQDSA 586

BLAST of MS001321 vs. ExPASy TrEMBL
Match: A0A6J1BQK8 (uncharacterized protein LOC111004847 OS=Momordica charantia OX=3673 GN=LOC111004847 PE=4 SV=1)

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 576/582 (98.97%), Postives = 579/582 (99.48%), Query Frame = 0

Query: 1   MAKMKKFQVKLQKLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR 60
           MAKMKKFQVKL+KLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR
Sbjct: 1   MAKMKKFQVKLEKLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR 60

Query: 61  FLNNGQETVEWDDEFHSICEFSAADDASIGSWDTNFYVLLENSTKSKTKSSVLGKASLNL 120
           FLNNGQETVEWDDEFHSICEFSAADDASIGSWDTNFYVLLENSTKSKTKSSVLGKASLNL
Sbjct: 61  FLNNGQETVEWDDEFHSICEFSAADDASIGSWDTNFYVLLENSTKSKTKSSVLGKASLNL 120

Query: 121 AEVLSEMEAKIDTILPITLKDAAGPRPATLSVSVRVNFVEVRDDPDPTRIARDPAQDEKD 180
           AEVLSEMEAKID ILPITLKDAAGPRPATL  SVRVNFVEVRDDPDPTRIARDPAQDEKD
Sbjct: 121 AEVLSEMEAKIDAILPITLKDAAGPRPATL--SVRVNFVEVRDDPDPTRIARDPAQDEKD 180

Query: 181 GFFRALKDLTSFKKKNRDKAISSDGENAVVGNRSEEEGDQKQGKLSWKKRRLSFSFRNSK 240
           GFFRALKDLTSFKKKNRDKAISSDGENAVVGNRSEEEGDQKQGKLSWKKRRLSFSFRNSK
Sbjct: 181 GFFRALKDLTSFKKKNRDKAISSDGENAVVGNRSEEEGDQKQGKLSWKKRRLSFSFRNSK 240

Query: 241 GKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTGVKTADQVSLELDGQNREGT 300
           GKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTGVKTADQVSLELDGQNREGT
Sbjct: 241 GKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTGVKTADQVSLELDGQNREGT 300

Query: 301 GGRWETREIVSRDGKAKLKTELFFGSFDQRSEKASGESACTAIVAVITHWLHSNQGAMPT 360
           GGRWETREIVSRDGKAKLKTELFFGSFDQRSEKA+GESACTAIVAVITHWLHSNQGAMPT
Sbjct: 301 GGRWETREIVSRDGKAKLKTELFFGSFDQRSEKANGESACTAIVAVITHWLHSNQGAMPT 360

Query: 361 RPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLPENSFVGFFSP 420
           RPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLPENSFVGFFSP
Sbjct: 361 RPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLPENSFVGFFSP 420

Query: 421 EKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVLEMEEDACYIVDSLG 480
           EKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVL+MEEDACYIVDSLG
Sbjct: 421 EKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVLKMEEDACYIVDSLG 480

Query: 481 ERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLAALTIEELEQE 540
           ERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLAALTIEELEQE
Sbjct: 481 ERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLAALTIEELEQE 540

Query: 541 QRKLSCNFIPHQRLQIDFHFSSPVDSSSSTSPFSVFFDEDSA 583
           QRKLSCNFIPHQRLQIDFHFSSPVDSSSSTSPFSVFFDEDSA
Sbjct: 541 QRKLSCNFIPHQRLQIDFHFSSPVDSSSSTSPFSVFFDEDSA 580

BLAST of MS001321 vs. ExPASy TrEMBL
Match: A0A6J1K753 (uncharacterized protein LOC111491769 OS=Cucurbita maxima OX=3661 GN=LOC111491769 PE=4 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 2.3e-233
Identity = 432/585 (73.85%), Postives = 492/585 (84.10%), Query Frame = 0

Query: 1   MAKMKKFQVKLQKLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR 60
           MAKMKKFQVKL  LKL+GFG E EA+AIEIKWKGPQ+HS+LSVPFYGKS LQ +RT  QR
Sbjct: 1   MAKMKKFQVKLGDLKLHGFGNEKEAIAIEIKWKGPQRHSILSVPFYGKSPLQTDRTTAQR 60

Query: 61  FLNNGQETVEWDDEFHSICEFSAADDASIGSWDTNFYVLLENSTKSKTKSSVLGKASLNL 120
            L    +T++WD EF SICEF  ADD+S  SWDT FYVLLE STKSKTK SVLGKASLNL
Sbjct: 61  PL---IQTLQWDHEFLSICEFEFADDSSTASWDTKFYVLLEESTKSKTKFSVLGKASLNL 120

Query: 121 AEVLSEMEAKIDTILPITLKDAAGPRPATLSVSVRVNFVEVRDDPDPTRIARDPAQDEKD 180
           A++L EME K++  +PI LKDA    P  +++SVRVNFVEVRDD D  +      Q +K+
Sbjct: 121 AKMLLEMETKVERNVPIMLKDA----PHQVTLSVRVNFVEVRDDSDTIQ------QQDKE 180

Query: 181 GFFRALKDLTSFKKKNRD--KAISSDGENAVVGNRSEEE-GDQKQ-GKLSWKKRRLSFSF 240
           GF +ALKDLTSF+KKNRD  KAISSDG+N  +G+  EE+ GDQKQ GKL  KKRRLSFSF
Sbjct: 181 GFLKALKDLTSFRKKNRDKGKAISSDGQNRALGDPKEEDGGDQKQLGKLLSKKRRLSFSF 240

Query: 241 RNSKGKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTGVKTADQVSLELDGQN 300
           R SKGKVEPWSEKT+TAVND V+VDRQQ D + +  P++  SQT    + ++SLE+D QN
Sbjct: 241 RYSKGKVEPWSEKTNTAVNDGVSVDRQQHDTEPTA-PISTTSQTEKGESTEISLEIDEQN 300

Query: 301 REGTGGRWETREIVSRDGKAKLKTELFFGSFDQRSEKASGESACTAIVAVITHWLHSNQG 360
           +E + GRWET+EIVSRDGKAKLKTE+FFGSFDQRSEKA GESACTAIVAVITHWLHSN G
Sbjct: 301 KEDSRGRWETKEIVSRDGKAKLKTEVFFGSFDQRSEKAGGESACTAIVAVITHWLHSNYG 360

Query: 361 AMPTRPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLPENSFVG 420
           AMPTRPELD+L+MEGSSEWQKLC+N CYS+SFPNKHFDLETV+EA+VRPITV  ENSFVG
Sbjct: 361 AMPTRPELDNLIMEGSSEWQKLCNNHCYSNSFPNKHFDLETVMEADVRPITVSAENSFVG 420

Query: 421 FFSPEKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVLEMEEDACYIV 480
           FFSPEKFNCL+EAMSFEQIW  V  N KT    YE R+YIVSWNDHFFVL+MEEDACYIV
Sbjct: 421 FFSPEKFNCLSEAMSFEQIWNLV--NTKTCPN-YETRVYIVSWNDHFFVLKMEEDACYIV 480

Query: 481 DSLGERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLAALTIEE 540
           DSLGERLFEGCNQAYILKFDSSSLM+E+KEEGE+ ELVC+GKECCREFF+RFLAA+TIEE
Sbjct: 481 DSLGERLFEGCNQAYILKFDSSSLMYESKEEGEVGELVCKGKECCREFFERFLAAITIEE 540

Query: 541 LEQEQRK-LSCNFIPHQRLQIDFHFSSPVDSSSSTSPFSVFFDED 581
           LE+EQ+K  SC+FIPHQRLQIDFHFSSPV SS STSP S+F D+D
Sbjct: 541 LEEEQKKQSSCSFIPHQRLQIDFHFSSPVVSSLSTSPCSIFSDQD 568

BLAST of MS001321 vs. ExPASy TrEMBL
Match: A0A6J1HEA9 (uncharacterized protein LOC111462700 OS=Cucurbita moschata OX=3662 GN=LOC111462700 PE=4 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.5e-232
Identity = 432/586 (73.72%), Postives = 489/586 (83.45%), Query Frame = 0

Query: 1   MAKMKKFQVKLQKLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR 60
           MAKMKKFQVKL  LKL+GFG E EA+AIEIKWKGP +HS+LSVPFYGKS LQ +RT  QR
Sbjct: 1   MAKMKKFQVKLGDLKLHGFGNEKEAIAIEIKWKGPHRHSILSVPFYGKSPLQTDRTTAQR 60

Query: 61  FLNNGQETVEWDDEFHSICEFSAADD-ASIGSWDTNFYVLLENSTKSKTKSSVLGKASLN 120
            L   Q+T++WD EF S+CEF  ADD +SI SWDT FYVLLE STKSKTK SVLGKASLN
Sbjct: 61  ALIQTQQTLQWDHEFLSVCEFEFADDSSSIASWDTKFYVLLEESTKSKTKFSVLGKASLN 120

Query: 121 LAEVLSEMEAKIDTILPITLKDAAGPRPATLSVSVRVNFVEVRDDPDPTRIARDPAQDEK 180
           LAE+L EME  ++  +PITLK A  P  ATL  SVRVNFVEVRDD DP +      Q +K
Sbjct: 121 LAEMLLEMETIVERNVPITLKGA--PHQATL--SVRVNFVEVRDDSDPIQ-----QQQDK 180

Query: 181 DGFFRALKDLTSFKKKNRD--KAISSDGENAVVGNRSEEE-GDQKQ-GKLSWKKRRLSFS 240
           +GF +ALKDLTSF+KKNRD  KA+SSDG+N    +  EE+ GDQKQ GKL  KKRRLSFS
Sbjct: 181 EGFLKALKDLTSFRKKNRDKGKAVSSDGQNRGFADPKEEDGGDQKQLGKLLSKKRRLSFS 240

Query: 241 FRNSKGKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTGVKTADQVSLELDGQ 300
           FR SKGKVEPWSEKT+TAVND V+VDRQQ D + +  P++  SQT    + ++SLE+D Q
Sbjct: 241 FRYSKGKVEPWSEKTNTAVNDGVSVDRQQHDTEPTA-PISITSQTEKGESTEISLEIDEQ 300

Query: 301 NREGTGGRWETREIVSRDGKAKLKTELFFGSFDQRSEKASGESACTAIVAVITHWLHSNQ 360
           N+E + GRWET+EIVSRDGKAKLKTE+FFGSFDQRSEKA GESACTAIVAVITHWLHSN 
Sbjct: 301 NKEDSRGRWETKEIVSRDGKAKLKTEVFFGSFDQRSEKAGGESACTAIVAVITHWLHSNY 360

Query: 361 GAMPTRPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLPENSFV 420
           GAMPTRPELD+L+MEGSSEWQKLC+N CYS+SFPNKHFDLETV+EA+VRPITV  ENSFV
Sbjct: 361 GAMPTRPELDNLIMEGSSEWQKLCNNHCYSNSFPNKHFDLETVMEADVRPITVSAENSFV 420

Query: 421 GFFSPEKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVLEMEEDACYI 480
           GFFSPEKFNCL+EAMSFEQIW E+ N  KT   Y   R+YIVSWNDHFFV +MEEDACYI
Sbjct: 421 GFFSPEKFNCLSEAMSFEQIWNEI-NTTKTCPNYV-TRVYIVSWNDHFFVFKMEEDACYI 480

Query: 481 VDSLGERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLAALTIE 540
           VDSLGERLFEGCNQAYILKFDSSSLM+E+KE+GE+ ELVC+GKECCREFF+RFLAA+TIE
Sbjct: 481 VDSLGERLFEGCNQAYILKFDSSSLMYESKEKGEVGELVCKGKECCREFFERFLAAITIE 540

Query: 541 ELEQEQRK-LSCNFIPHQRLQIDFHFSSPVDSSSSTSPFSVFFDED 581
           ELE+EQ K  SCNFIPHQRLQIDFHFSSPV SS STSP S+F D+D
Sbjct: 541 ELEEEQEKQSSCNFIPHQRLQIDFHFSSPVVSSLSTSPCSIFSDQD 574

BLAST of MS001321 vs. ExPASy TrEMBL
Match: A0A5A7VGR1 (C2 NT-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002390 PE=4 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 3.7e-228
Identity = 428/593 (72.18%), Postives = 483/593 (81.45%), Query Frame = 0

Query: 1   MAKMKKFQVKLQKLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR 60
           MAKMKKFQVKL  LKLYG+ KENE +AIEIKWKGPQ+HSLLSVPFY KS LQ NRT  Q 
Sbjct: 1   MAKMKKFQVKLDNLKLYGYDKENERIAIEIKWKGPQRHSLLSVPFYAKSPLQINRTSAQP 60

Query: 61  FLNNGQETVEWDDEFHSICEFS---AADDASIGSWDTNFYVLLENSTKSKTKSSVLGKAS 120
            LNN     +W+ EFHSIC F      D +SI  WDT FYVLLE STKSKTK+SVLGKAS
Sbjct: 61  VLNNHH---QWNHEFHSICAFEFPHHEDPSSIPFWDTKFYVLLEESTKSKTKTSVLGKAS 120

Query: 121 LNLAEVLSEMEAKIDTILPITLKDAAGPRPATLSVSVRVNFVEVRDDPDPTRIARDPAQD 180
           LNLAE+L  +E K++  +PITLKD+AG  P    +SV VNFVE+RD  D         Q 
Sbjct: 121 LNLAEMLLALERKMERNVPITLKDSAGAAPHHAMISVCVNFVEIRDGSDMIH------QQ 180

Query: 181 EKDGFFRALKDLTSFKKKNRDKA--ISSDGENAVVGNRS--EEEGDQKQGKLSWKKRRLS 240
           +K+GF +ALK LTSFKKKNR+K   ISSDGEN  +G+ +  E++GDQK GKL  KKRRLS
Sbjct: 181 DKEGFMKALKGLTSFKKKNREKGKMISSDGENRGLGDPTIGEDDGDQKLGKLFSKKRRLS 240

Query: 241 FSFRNSKGKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTG--VKT-ADQVSL 300
           FSFR+SKGKVEPW EKT+TAVND VTVD+QQ D D SV    PIS T    KT +   SL
Sbjct: 241 FSFRHSKGKVEPWLEKTNTAVNDGVTVDQQQHDNDPSVLKDVPISTTSQMEKTESTAFSL 300

Query: 301 ELDGQNREGTGGRWETREIVSRDGKAKLKTELFFGSFDQRSEKASGESACTAIVAVITHW 360
           E DGQN+E +GG+WETREIVSRDGK KLKTE+FFGSFDQRSEKA GESACTAIVAVITHW
Sbjct: 301 ETDGQNKETSGGKWETREIVSRDGKTKLKTEVFFGSFDQRSEKAGGESACTAIVAVITHW 360

Query: 361 LHSNQGAMPTRPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLP 420
           LHSN G MPT+ ELD+L+MEGSSEWQKLC+N CYS+ FPNKHFDLETVV+A+VRPI V P
Sbjct: 361 LHSNYGMMPTQQELDNLIMEGSSEWQKLCNNVCYSNYFPNKHFDLETVVQADVRPIAVSP 420

Query: 421 ENSFVGFFSPEKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVLEMEE 480
           E+SFVGFFSPEKF+CLTEAMSFEQIW EV  NAKT +  YE RIYIVSWNDHFFV++MEE
Sbjct: 421 EDSFVGFFSPEKFSCLTEAMSFEQIWNEV--NAKTCS-IYESRIYIVSWNDHFFVMKMEE 480

Query: 481 DACYIVDSLGERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLA 540
           DACYI+DSLGERLFEGCNQAYILKFDSSSLMFEN+E+GE  ELVCRGKECCREFF+RFLA
Sbjct: 481 DACYIIDSLGERLFEGCNQAYILKFDSSSLMFENQEKGEAGELVCRGKECCREFFERFLA 540

Query: 541 ALTIEELEQEQRKLS-CNFIPHQRLQIDFHFSSPVDSSSSTSPFSVFFDEDSA 583
           A+TIEELE+EQ+KLS  NF+PHQRLQIDFHFSSPV SSSSTSP+S+F D+  A
Sbjct: 541 AITIEELEEEQKKLSDFNFVPHQRLQIDFHFSSPVASSSSTSPYSLFSDQSPA 581

BLAST of MS001321 vs. ExPASy TrEMBL
Match: A0A1S3BBP9 (uncharacterized protein LOC103488316 OS=Cucumis melo OX=3656 GN=LOC103488316 PE=4 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 3.7e-228
Identity = 428/593 (72.18%), Postives = 483/593 (81.45%), Query Frame = 0

Query: 1   MAKMKKFQVKLQKLKLYGFGKENEAVAIEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQR 60
           MAKMKKFQVKL  LKLYG+ KENE +AIEIKWKGPQ+HSLLSVPFY KS LQ NRT  Q 
Sbjct: 1   MAKMKKFQVKLDNLKLYGYDKENERIAIEIKWKGPQRHSLLSVPFYAKSPLQINRTSAQP 60

Query: 61  FLNNGQETVEWDDEFHSICEFS---AADDASIGSWDTNFYVLLENSTKSKTKSSVLGKAS 120
            LNN     +W+ EFHSIC F      D +SI  WDT FYVLLE STKSKTK+SVLGKAS
Sbjct: 61  VLNNHH---QWNHEFHSICAFEFPHHEDPSSIPFWDTKFYVLLEESTKSKTKTSVLGKAS 120

Query: 121 LNLAEVLSEMEAKIDTILPITLKDAAGPRPATLSVSVRVNFVEVRDDPDPTRIARDPAQD 180
           LNLAE+L  +E K++  +PITLKD+AG  P    +SV VNFVE+RD  D         Q 
Sbjct: 121 LNLAEMLLALERKMERNVPITLKDSAGAAPHHAMISVCVNFVEIRDGSDMIH------QQ 180

Query: 181 EKDGFFRALKDLTSFKKKNRDKA--ISSDGENAVVGNRS--EEEGDQKQGKLSWKKRRLS 240
           +K+GF +ALK LTSFKKKNR+K   ISSDGEN  +G+ +  E++GDQK GKL  KKRRLS
Sbjct: 181 DKEGFMKALKGLTSFKKKNREKGKMISSDGENRGLGDPTIGEDDGDQKLGKLFSKKRRLS 240

Query: 241 FSFRNSKGKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTG--VKT-ADQVSL 300
           FSFR+SKGKVEPW EKT+TAVND VTVD+QQ D D SV    PIS T    KT +   SL
Sbjct: 241 FSFRHSKGKVEPWLEKTNTAVNDGVTVDQQQHDNDPSVLKDVPISTTSQMEKTESTAFSL 300

Query: 301 ELDGQNREGTGGRWETREIVSRDGKAKLKTELFFGSFDQRSEKASGESACTAIVAVITHW 360
           E DGQN+E +GG+WETREIVSRDGK KLKTE+FFGSFDQRSEKA GESACTAIVAVITHW
Sbjct: 301 ETDGQNKETSGGKWETREIVSRDGKTKLKTEVFFGSFDQRSEKAGGESACTAIVAVITHW 360

Query: 361 LHSNQGAMPTRPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEAEVRPITVLP 420
           LHSN G MPT+ ELD+L+MEGSSEWQKLC+N CYS+ FPNKHFDLETVV+A+VRPI V P
Sbjct: 361 LHSNYGMMPTQQELDNLIMEGSSEWQKLCNNVCYSNYFPNKHFDLETVVQADVRPIAVSP 420

Query: 421 ENSFVGFFSPEKFNCLTEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVLEMEE 480
           E+SFVGFFSPEKF+CLTEAMSFEQIW EV  NAKT +  YE RIYIVSWNDHFFV++MEE
Sbjct: 421 EDSFVGFFSPEKFSCLTEAMSFEQIWNEV--NAKTCS-IYESRIYIVSWNDHFFVMKMEE 480

Query: 481 DACYIVDSLGERLFEGCNQAYILKFDSSSLMFENKEEGEIKELVCRGKECCREFFKRFLA 540
           DACYI+DSLGERLFEGCNQAYILKFDSSSLMFEN+E+GE  ELVCRGKECCREFF+RFLA
Sbjct: 481 DACYIIDSLGERLFEGCNQAYILKFDSSSLMFENQEKGEAGELVCRGKECCREFFERFLA 540

Query: 541 ALTIEELEQEQRKLS-CNFIPHQRLQIDFHFSSPVDSSSSTSPFSVFFDEDSA 583
           A+TIEELE+EQ+KLS  NF+PHQRLQIDFHFSSPV SSSSTSP+S+F D+  A
Sbjct: 541 AITIEELEEEQKKLSDFNFVPHQRLQIDFHFSSPVASSSSTSPYSLFSDQSPA 581

BLAST of MS001321 vs. TAIR 10
Match: AT5G04860.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G11760.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 315.5 bits (807), Expect = 9.2e-86
Identity = 234/694 (33.72%), Postives = 327/694 (47.12%), Query Frame = 0

Query: 28  IEIKWKGPQKHSLLSVPFYGKSSLQRNRTRPQRFLNNGQETVEWDDEFHSICEFSAADDA 87
           +EIKWKGP+  +L       K S+ RN T    F  +G   VEW++EF  +CEFS   + 
Sbjct: 62  VEIKWKGPKSVTL-------KRSVVRNLTEEGGFRGDG--VVEWNEEFKRVCEFSVYKEG 121

Query: 88  SIGSWDTNFYVLLENSTKSKTKSSVLGKASLNLAEVLSEMEAKIDTILPITLKDAAGPRP 147
           S   W  +  V    +  SK K    GKASLN+AE  S M+ + D  + + LKD      
Sbjct: 122 SFLPWFVSLTVFSGLNQGSKEKVRSFGKASLNIAEYFSLMK-EDDVQVKVPLKDCDSSSV 181

Query: 148 ATLSVSVRVNFVEVRDDPDPTRIA------------------------------------ 207
            +  V + + F      P+  R A                                    
Sbjct: 182 RSPHVHISLQFSPKESLPERQRSALPVLWSPLSAEAEKAESVVKVGLRKMKTFNNCMSST 241

Query: 208 -RDPAQDEKDGFFRALKDLTSFKKK-NRDKAISSDGENAVVGNRSEEEGDQKQGK----- 267
                + EKDG   +  D  S ++  + D +   D ++   G+ ++E  + K+ +     
Sbjct: 242 QASEKESEKDGSSGSGSDGKSPERNLDSDSSYPFDTDSLDEGDAADESEENKENESSLAD 301

Query: 268 ------------------------------------------------------------ 327
                                                                       
Sbjct: 302 PVNYKTLRSANWARGSFHTVTNPEDEDLIYYSHRSPLAETGHCSDEVSNDVVSLEQAKGQ 361

Query: 328 ------LSWKKRRLSFSFRNSKGKVEPWSEKTDTAV-NDAVTVDRQQ----DDKDSSVFP 387
                 LSWKKR+L  SFR+ K K EP  +K       D +  DR+Q    D+ +S  + 
Sbjct: 362 MSKKRMLSWKKRKL--SFRSPKQKGEPLLKKDCLEEGGDDIDFDRRQLSSSDESNSDWYR 421

Query: 388 -----VAPISQTGVKTADQVSLELDGQNREGTGGRWETREIVSRDGKAKLKTELFFGSFD 447
                + P+SQ G              + +   G WET+EI+SRDG  KL   +F  S D
Sbjct: 422 SDDAIMKPLSQFG--------------DDDFVVGSWETKEIISRDGLMKLTARVFLASID 481

Query: 448 QRSEKASGESACTAIVAVITHWLHSNQGAMPTRPELDSLVMEGSSEWQKLCSNDCYSSSF 507
           QRSE+A+GESACTA+VAV+ HWL SN+  +PTR E DSL+ EGSSEW+ +C N+ Y   F
Sbjct: 482 QRSERAAGESACTALVAVMAHWLGSNRDIIPTRSEFDSLIREGSSEWRNMCENEEYRERF 541

Query: 508 PNKHFDLETVVEAEVRPITVLPENSFVGFFSPEK-----------FNCLTEAMSFEQIWK 562
           P+KHFDLETV++A+VRPI V+PE SF+GFF PEK            + L   MSF+ IW+
Sbjct: 542 PDKHFDLETVLQAKVRPICVVPERSFIGFFHPEKSEEEEGKEDASLDFLKGVMSFDSIWE 601

BLAST of MS001321 vs. TAIR 10
Match: AT3G11760.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G04860.1); Has 84 Blast hits to 73 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 84; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 295.8 bits (756), Expect = 7.5e-80
Identity = 164/387 (42.38%), Postives = 238/387 (61.50%), Query Frame = 0

Query: 203 SDGENAVVGNRSEEEGDQKQGKLSWKKRRLSFSFRNSKGKVEPWSEKTD-TAVNDAVTVD 262
           SD E++  G   E     ++  L W+KR+L  SFR+ K K EP  +K +     D +  D
Sbjct: 318 SDAEDSAAGLVYEASLLPRRSILPWRKRKL--SFRSPKSKGEPLLKKDNGEEGGDDIDFD 377

Query: 263 RQQDDKDSSVFPVAPISQTGVKTADQVSLELDGQNREGTGGRWETREIVSRDGKAKLKTE 322
           R+Q   D +  P             + S    G++    G  WE +E++SRDG  KL+T 
Sbjct: 378 RRQLSSDEAHPPFGSKIDEDSSANPRTSFSEFGEDSFAIGS-WEEKEVISRDGHMKLQTS 437

Query: 323 LFFGSFDQRSEKASGESACTAIVAVITHWLHSNQGAMPTRPELDSLVMEGSSEWQKLCSN 382
           +F  S DQRSE+A+GESACTA+VAVI  W   N   MP + + DSL+ EGS EW+ LC N
Sbjct: 438 VFLASIDQRSERAAGESACTALVAVIADWFQKNGNLMPIKSQFDSLIREGSLEWRNLCEN 497

Query: 383 DCYSSSFPNKHFDLETVVEAEVRPITVLPENSFVGFFSPE------KFNCLTEAMSFEQI 442
           + Y   FP+KHFDL+TV++A++RP+TV+P  SFVGFF P+      +F  L  AMSF+ I
Sbjct: 498 ETYMQKFPDKHFDLDTVLQAKIRPLTVIPGKSFVGFFHPDGMINEGRFEFLQGAMSFDSI 557

Query: 443 WKEVNNNAKTSTG--YYE----PRIYIVSWNDHFFVLEMEEDACYIVDSLGERLFEGCNQ 502
           W E+ +  ++S     Y+    P +YIVSWNDHFFVL++E++A YI+D+LGERL+EGC+Q
Sbjct: 558 WAEIISLEESSANGDSYDDDSPPHVYIVSWNDHFFVLKVEKEAYYIIDTLGERLYEGCDQ 617

Query: 503 AYILKFDSSSLMFE-------NKEEGEIKELVCRGKECCREFFKRFLAALTIEELEQEQR 562
           AY+LKFD  +++ +         E     E++ RGKE C+E+ K FLAA+ I EL+++ +
Sbjct: 618 AYVLKFDHKTVIHKILHTEEAGSESEPESEILSRGKESCKEYIKNFLAAIPIRELQEDIK 677

Query: 563 KLSCNFIP-HQRLQIDFHFSSPVDSSS 569
           K   +  P H RLQI+FH+++  + +S
Sbjct: 678 KGLASTAPVHHRLQIEFHYTTNAEITS 701

BLAST of MS001321 vs. TAIR 10
Match: AT2G25460.1 (CONTAINS InterPro DOMAIN/s: C2 calcium-dependent membrane targeting (InterPro:IPR000008); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G04860.1); Has 108 Blast hits to 69 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 108; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 229.6 bits (584), Expect = 6.6e-60
Identity = 158/459 (34.42%), Postives = 237/459 (51.63%), Query Frame = 0

Query: 3   KMKKFQVKLQKLKLYGF--------GKENEAVAIEIKWKGP-QKHSLLSVPFYGKSSLQR 62
           K++K  V ++ ++L G           +N +  +E+KWKGP     L  VPFY +S+   
Sbjct: 4   KVRKLHVTVKPVRLDGLPAILGDETAGKNLSAMVEVKWKGPVSGFGLGFVPFY-RSNRPV 63

Query: 63  NRTRPQRFLNNGQETVEWDDEFHSICEFSAADDASIGSWDTNFYVLLENSTKSKTKSSVL 122
           N T   + +  G   VEW++EF  +C         +G W+ +F V    +  +K K S++
Sbjct: 64  NHT-SSKPIALGSNHVEWEEEFERVC-------CIVGPWNLSFNVFYGENMDAKNKKSLI 123

Query: 123 GKASLNLAEVLSEMEAKIDTILPITLKDAAGPRPATLSVSVRVNFVEVRDDPDP----TR 182
           GKASL+L+E+ S+ E+ ++  LPI  K +   + ATL V+  V F EVR +PD      +
Sbjct: 124 GKASLDLSELASKQESTVERKLPIRSKGSVLSKEATLVVN--VTFSEVRTEPDDFMQLGQ 183

Query: 183 IARDPAQDEKDGFFRALKDLTSFKKKNRDKAISSDGENAVV-------GNRSEEEGDQKQ 242
           I+ D A  +K    R           +   A SS G + VV        + ++ E  QK 
Sbjct: 184 ISVDSAIPKKKSTSRRGGGSHFDSFSSPATASSSGGRSPVVETGLFSSPDENQSEPGQKA 243

Query: 243 GKLSWKKRRLSFSFRNSKGKVEPWSEKTDTAVNDAVTVDRQQDDKDSSVFPVAPISQTGV 302
           G   WK+RRLSFS    +   E    KT T  ++ +                        
Sbjct: 244 GFNWWKRRRLSFSMTWRREPREDEVTKTSTKPSEELE----------------------- 303

Query: 303 KTADQVSLELDGQNREGTGGRWETREIVSRDGKAKLKTELFFGSFDQRSEKASGESACTA 362
           K A ++ +E           +W  +++VSRDGK+KLK+E++  S DQRSE+A+GE+AC A
Sbjct: 304 KPATEIPIE---------ANKWVMKDLVSRDGKSKLKSEVYLASIDQRSEQAAGEAACAA 363

Query: 363 IVAVITHWLHSNQGAM-PTRPELDSLVMEGSSEWQKLCSNDCYSSSFPNKHFDLETVVEA 422
           +  V+ HW H+N   + P+    DSL+ +GSS WQ LC  + Y   FPN+HFDLET+V A
Sbjct: 364 VAVVVAHWFHANPKLINPSGTAFDSLITQGSSLWQSLCDKESYLRLFPNRHFDLETIVSA 419

Query: 423 EVRPITVLPENSFVGFFSPEKFNCLTEAMSFEQIWKEVN 441
            +RP+ V  + SF G FSPE+F  L   MSF+QIW E++
Sbjct: 424 NLRPVRVCTDKSFTGLFSPERFASLDGLMSFDQIWDELS 419

BLAST of MS001321 vs. TAIR 10
Match: AT2G10560.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G04860.1); Has 70 Blast hits to 70 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 70; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 167.9 bits (424), Expect = 2.4e-41
Identity = 95/227 (41.85%), Postives = 136/227 (59.91%), Query Frame = 0

Query: 378 LCSNDCYSSSFPNKHFDLETVVEAEVRPITVLPENSFVGFFSPEK-----------FNCL 437
           +C N+ Y   FP+KHFDLETV++A+VRPI V+PE +F+GFF  EK            + L
Sbjct: 1   MCENEEYRERFPDKHFDLETVLQAKVRPICVVPERTFIGFFHREKSKEEEEKEDVSLDFL 60

Query: 438 TEAMSFEQIWKEVNNNAKTSTGYYEPRIYIVSWNDHFFVLEMEEDACYIVDSLGERLFEG 497
              MSF+ IW+E+       +   E  IYIVSWNDH+FVL +  DA YI+D+LGER++EG
Sbjct: 61  KGVMSFDSIWEEIMKQEPEESA-SEHVIYIVSWNDHYFVLLVNHDAYYIIDTLGERVYEG 120

Query: 498 CNQAYILKFDSS-------SLMFENK------------------------EEGEIKELVC 557
           CNQAY+LKFD         S++ +NK                        E+GE + +VC
Sbjct: 121 CNQAYVLKFDQDAEIKRLPSVIKDNKADMGSQKQGGKNKYEQPERSKESEEQGE-EVVVC 180

Query: 558 RGKECCREFFKRFLAALTIEELEQEQRK-LSCNFIPHQRLQIDFHFS 562
           RGKE CRE+ K FLAA+ I++++ + ++ L  +F  H RLQI+ +++
Sbjct: 181 RGKESCREYIKSFLAAIPIQQVKADMKEGLVSSF--HHRLQIELYYT 223