MS001295 (gene) Bitter gourd (TR) v1

Overview


Name	MS001295
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	tRNA (Cytosine(38)-C(5))-methyltransferase
Location	scaffold36: 3042680 .. 3048717 (-)
RNA-Seq Expression	MS001295
Synteny	MS001295

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAGGTTTCTCCGCAGCTGCAAGGCGAACCATGGAGAGTCCTTGAATTCTACAGCGGCATTGGTGGCATGGTACTATTCTTCCCCACGCCTCTCTCTTCCTCCATCTTTATCATGTGTATATCTTTCTTTAGAACCGTTGTTTGCTAATCGAATTCAAACAGAGGTACTCGCTACTGAGTGCTGGCGTCGATGCGAAAGTGGTACAAGCTTTTGATATCAACGACAAAGCAAACGACGTTTACGAGCACAATTTCGGCGATCGTCCGTATCAGGTATGACAGTGTCTTCTTCTTATTTTGTTTCTTGAGAATGATTCTATTTCTATCGTACGAGTATTTTTTCGCTGCTTCTCAATTCGAACAATGAAATTGGTGTTTCCTTCAAAATGGAGATGAAAAAATTATTTCCAGTCGTGGTTTAAGTAGATTGAATTTGAAGTCGTTGAATGTTGGAGTTAAGCTTATCTAGTTGGTGAGCAATTACCTCAGAATCATCTAGTAAGCTGACGATGCGTTAGCTATCTTAAGTGTTTTTCTGTTGGAATATTCGATTCAAATGACTAGCTCTGTGGATTTTCTTTTGAACTGCCAAACCATAACCGTCGTACAATTTTTCGACAGCGTCTTGGCGATTCATTCTGTATCGCAGGAGTAAATTTTTTGCTGCTTCTCAACAGAGACAACGAAACTATTTTGTTTTTTCAAAATTGAGATGAAAATACTTTCCGGTTGTGGTTTAAGTATATTGAATGTTGAAACTAAGCTTATTTATTTCGTTAGGAATTACCTTCCACTCACTAAAGGACGTATTTCTTAAGTGATAAGATTACAAATTGCAGCTAGTTTGAATTCACAACGTAAATGGAAAGTATGGTAGCAATATGAAGTATTTTTGCTGGAATCTCTGATTCAAACAACTTTCGTTGTGGTTTTTTTTTTCGAACTGCTAAACCAAAAGCGTCATAAAATTTAGGACATGTAACAGCTCATGCGAAGCTTCCTTTGAAATGGAGTGTCAATTTTTGCTTATAAGTTTAGTTATGGCCAAAAGCAACCCTATGTGCTTGGTTGGAAGTTTGGAGGTTCAGGAAAGTAAAGAATTATTGATTTTTGAGACCAGTCCTGCGTCTTTCTTGTAGCTCTCTTTTGATATCTGAATCTTTCCAGTAGCTCTCATTTGATTTCCCTGCTACATAGGTCTGGCATCCTTAGTCGTTGGATCTATTATCTAATTTGGAGTCTGGACATTTAGGATACCGAAGAAATTCTTATATTTCGTGTCCATATTAGTCCTCGGAGGTTAGACATGGCATTTGTGTATTGGTTTTGCATGATAAAAGTGTGGGTGGGTGGATGCCATGGGAAATCGTGGTCTCCTTTGACTTTTGAGTTATTCCTTCTCAAGTTAGAGTTGATTTTGGCACGATTTTCTGGCTTCACCTCCAAAAAAGAAGGGTCGAAATTGTTTACCATTACATTGCTTCGTTAGTATGTGCCTTATTTGGAATGTTAGAAATAGGAAAAGCTTTTAGCAGTAGGCAGAATATTATTTGGCTTTCTAATAACACTGAAGAGTTCTATAATTGAACAGTTGGAGAGCTTGTTTCCCTCTCCTTCTCGTTGGATTCTGTTAGATTAGACCACTTATCACCTTCTATATTTCTTTGGTTGCAATAAATTGACTTCCTGTACTTCGTTCAAGTGGACAAAGAGAAGATATGAATGTTTGTGAATGTTGATGCAATGCATGCAAATGATTGGTTCAGTTATATTTGTGCAGTTGGCTTTTGCAGTCAAGACTAACTTTTACTAGGGCTATTATCTTGGCAAGTACAAAACAGAGCATACAGCTTAGTTTGCATAATATTGGGATAAACTTTGATGTTCTAAAATAAATTGACCATATTACTCTTTTCTAAGTACTTTATTTGGATAGGGTAATATTGAAAGTCTGACTGCTGCTGATATCGACAGCTACCAAGCTCATGTTTGGCTTCTTTCACCTCCTTGCCAACCGTATACACGACAAGGTGTGTTTTCGTATCAATTTAGAAATAAATAAATTATCAATATCATCTGCTGTTTAGTTAAAGAAATTTTGTGGTAAATTTTACATACTGAAAGTACATTCCTTGGTTATTAATATCAAATTGTATTCGACCAGTTTCAAGTTCATGGTTTCGCTGAATGCTACCATTGAATGTTTTTCTTCCCCAATAAAAGCACTGTGATAAAGTGAGAAATTTGTACTTCTCAACTGTCATTGGGGAACATGCTTTATCATTGAACTAGCTAGATATTAGATACTTTGTTTTTTTTTATTTTTTAACTAAGTTTGAGTTTCTTACTAATTTTCGGAAAATTATGATAAACGATTTCCAAGTATTTGAACTTCACGGGAATACTGTCTTTAAGCATAAATTATGGCAGCTACATGACAGCATGTTAGCACAGTTAAATGTTATTTTATATTTTTTTCAACGATGTAATATATTTTAGACAACAAATCCGAATGTATCTTTAGCTTGAAATATATAAAACTATGAAATGAGATTGATTTTTATTTTTATTTTTTTAAAAAAACCTTATTAAGGAGATGTTGGACAGTTGTTTTTGGCCCATTTTGTCCATTCTTTCAATTAACGATTTTCATATATCCCTGCATGGCTACATTTAACTACTTCTGCCTTTCATTTCGTACTCATAATAGAGTACTAGTATTTTGAAGGCACCATCTAAATAGAATTTCATTGATTAGTAGCCAAAGAGATTTGAACAGTATTTTTCCGTGCTTGGTCTGTATCTGGAACATAATAACTTAAATGCCCATTGGAAAAGTGACGCACTGGGTACATTCCTTTACTCAGTTTCTTGGAAAATCTCTCTCCCCCCACCCCCACTAACACAAATAAAATAAAATAAAAGATATCAAGAACAATCTGGCCCAAATTACTTGCTACATTTTATGCATGTAAATCATGCATCTTATAAACTGGAGAGTGGATGATCGTGCCTTATATAAATAGGTCTACAAAAGCATTCTGGCGATGCTCGGGCATTTTCATTCCTCAAAATTCTTGAGTTGATGCTACAAACGTCAAGGCCACCAGTAATGTTATTCATGGAAAACGTTGTTGGATTTGAGGTATGTTACTTGAACTTATTGGTGTACTGGAATTTATTTGATTGATTCTTCATCTTTTTTCAGATGCTTTCCTTTCTGTTGCAGACATCTGACACACATACAAAAATGATTGAGATATTGGAAAAAGCTGATTTTTCTAGACAGGAGTTTATTTTAAGCCCAATGCAATTTGGTGTTCCATATTCTAGGCCACGATACTTCTGCCTGGTACCGTACTTTCTTCACTTCCTTTCCTCTCCATGCTGTTATTGTTAAACAGATTCCTTTCCAATTTAGTTCCACCAATATCACAATGTCATGGAGCTTTTAATGCCATTAAAAATAGCCAGTCAGAATAGCTGAGTATCGTTTAAACTTTCTGCCTTTGGTGATTCGTAATTATAACACAACATCAAAACTTCCAAGGAAGAACACCATTTAGTCTACGAGGTTTAAGAAGATTCATCTCTTAAGTCAAAAAATTGTCATTTGAACCCATAAAGTTCAGAAAAAGAACATTAAGACTGCAGGTTTTCAGGGGAAAATCCTCATCTAATGACAAGCAAGATGAAGAAGAATGGAGAGAAGGGGGCATAGAAAAAGAAGTACCCAGAAACATTCAGCTAATGGTGGGCCTACTCGATGCACTCTTAAAATACCCTTGTGTAATACTTCTGAAGACACCAACTGGCCAACCAAGTATTGAAAAATAGTTGAATTGTTTCCATATAGAAACTAAAGAAAAGGATTGTGATTGTGCCATCAGCATGGTTATCTTGATAAGTGCCTTACCTTTAGCCATTTGCTTTAGACCCACGAGAGTCTTCTAAATTAGAAGCAAGGCTACAGAATTGGCAAATCAAGGTCAAATACAACCACAACTGCCACGAAGTAAAAGATGAAAAATAATGAAAGCATCTAGGAAATTACTTAGCTAACAGTAGGTGACAGCTTGAGGAAGACGAGGGATGAAGATCCTATCCCTCTGTTTCTGATGTTGTGGATTTTGTGGTTCTTTATTAGTTTGTGCCTACTTCAACAACAATGAAGAAAGAGATAATGAAGAACAAGGCAGAGGGGCAGGAATTGGTGTTGTGGTATTTTATTTTGGAACCACTTCTTTAATAAATAAGCAGGTAAATATGCTGCATAATCCAAAAACTAGGAGGGAACATAGTTCTGCTATGTATTGAAAATAGACCATAGAGGATGCACCATAAGAGGTTTCAATTGCTCATCGTTTTTCTATTAAGATTCATAAAAGGCACAAAAATCCGAGGACCATTTGAAACCATTTTCTAAACTTTAGGGTTAAAGTTCGCTTTTAGGTACAAAATAGAGTCATTTCGGATTGACTGAGAAGGCATTTTGTACAAACTTCTACAGATGTGACATCATAACTCATCATGAGCTGTTCTCTTATCAACTCAATGGTAGTATGTTCTTTCACTTTATTTTCCACATGAAATTGTGGCATGGTCATGCAGCAATACAACATCGAACGTATAAAAAATAATCAGTTTGCTGGTGAAGGTTCATGCCTGTAATATGGATTTCTTTACTATGCGTCTGTGAATTTAGTGTTTGAAGGCAGATCTTATCATTCATTTTTCACTATATTTTTAGATTATTGGCTATTAAGAAAATAAATGTACTTTTTGTAGCTTCTAAATTTAGCATCCTTGTACTTTTGTAGGCTAAAAGGAGGCCTTTATCCTTTCACAGGCTATTATACAATAATCAACTTCTGTGGTCTCCAAGCCCATTACTTGAGAATGATGTTGACATAGAAACCATTGAAAATCATGGATCACAAGCGACTTCTGATAAGTTGCTGCTCTCTTGTGAACCAATATGTAGGTTTCTTGATCATTCCAGCCATCACAGTGAATTTAGTTTTTCTGATGCCAGTAGTATTCAAAGAGAGGGTGCATTGGAGAAAACCAAGGATACAGATGAGCAAGGATCTTTTGATCAGTATCTCGTCCCATCCAGTTTGATAGACAGATGGGGCAGTGCTATGGGTATCCAGACATATTTTATGTTCAATTGTTATAGATCTGTTTTGTCTCATCAACAGAACTTGTTATAGACTCTGCAATAGATCCTTAAGAGGGAGTTAGATATTGTGTATCCTGATTCAAGGCGATGCTGCTGTTTCACAAAAAGCTACTACCGATACGTGAAGGGTACTGGTTCACTTTTGGCACCTTTTGAGGTATAATTTTATCCTTAACATAACTGTGTCGAATATGGGATCTTGCACCTTCCTTAAATTCTTAATAACAGATAGCTAAATCCTAAGTTTCAAATATCATAACTAAATATAATTCCGTATATTTTTTGAGTTAGTTAAATAATGTATGTTTGTACTTTGTATGACAGACTTTCACCTGAATTTGTGAAGATATGCGATTCTGATTTGAGGCAATTGACATTTTTTGAACCTGGAAAAGAATCATGTTCAAACTTGATACTGCATGTTAATAAGTGAACAAACAAATCCTTTATAGTTCCCCTTGTATTTAGATTTTTATTTTTATTTAAAACCATAGTGTTTTGCAACTTCCCCCCGTTTTTGTTGGTCAGCAGTGATATTCGTCCATCTAGTATAAAAATATGCAACTTTTCTTGTAACATCATCATCCCACTTTTCTCACTGACTGGTAATGCAGGAAGAAAAGATGGGCAAGGCTCATTCTCTGAAAGAACAGAAGCTCAGATATTTTACTCCTAGAGAGGTCCTTGATGATTTTATGTTAAAATGATGCTTCTTTTGATTTCATTCTCAAGCTCACGCTGTTTATTAATTTTTCGATTTTGTTTCTTATTAATATTAGGTTGCTAACTTGCATTCCTTTCCGGAAGATTTTCAATTTCCACAGCATATCGGTCTTCGGCAACGGTAT

mRNA sequence

ATGGAGAAGGTTTCTCCGCAGCTGCAAGGCGAACCATGGAGAGTCCTTGAATTCTACAGCGGCATTGGTGGCATGAGGTACTCGCTACTGAGTGCTGGCGTCGATGCGAAAGTGGTACAAGCTTTTGATATCAACGACAAAGCAAACGACGTTTACGAGCACAATTTCGGCGATCGTCCGTATCAGGGTAATATTGAAAGTCTGACTGCTGCTGATATCGACAGCTACCAAGCTCATGTTTGGCTTCTTTCACCTCCTTGCCAACCGTATACACGACAAGGTCTACAAAAGCATTCTGGCGATGCTCGGGCATTTTCATTCCTCAAAATTCTTGAGTTGATGCTACAAACGTCAAGGCCACCAGTAATGTTATTCATGGAAAACGTTGTTGGATTTGAGACATCTGACACACATACAAAAATGATTGAGATATTGGAAAAAGCTGATTTTTCTAGACAGGAGTTTATTTTAAGCCCAATGCAATTTGGTGTTCCATATTCTAGGCCACGATACTTCTGCCTGGCTAAAAGGAGGCCTTTATCCTTTCACAGGCTATTATACAATAATCAACTTCTGTGGTCTCCAAGCCCATTACTTGAGAATGATGTTGACATAGAAACCATTGAAAATCATGGATCACAAGCGACTTCTGATAAGTTGCTGCTCTCTTGTGAACCAATATGTAGGTTTCTTGATCATTCCAGCCATCACAGTGAATTTAGTTTTTCTGATGCCAGTAGTATTCAAAGAGAGGGTGCATTGGAGAAAACCAAGGATACAGATGAGCAAGGATCTTTTGATCAGTATCTCGTCCCATCCAGTTTGATAGACAGATGGGGCAGTGCTATGGATATTGTGTATCCTGATTCAAGGCGATGCTGCTGTTTCACAAAAAGCTACTACCGATACGTGAAGGGTACTGGTTCACTTTTGGCACCTTTTGAGGAAGAAAAGATGGGCAAGGCTCATTCTCTGAAAGAACAGAAGCTCAGATATTTTACTCCTAGAGAGGTTGCTAACTTGCATTCCTTTCCGGAAGATTTTCAATTTCCACAGCATATCGGTCTTCGGCAACGGTAT

Coding sequence (CDS)

ATGGAGAAGGTTTCTCCGCAGCTGCAAGGCGAACCATGGAGAGTCCTTGAATTCTACAGCGGCATTGGTGGCATGAGGTACTCGCTACTGAGTGCTGGCGTCGATGCGAAAGTGGTACAAGCTTTTGATATCAACGACAAAGCAAACGACGTTTACGAGCACAATTTCGGCGATCGTCCGTATCAGGGTAATATTGAAAGTCTGACTGCTGCTGATATCGACAGCTACCAAGCTCATGTTTGGCTTCTTTCACCTCCTTGCCAACCGTATACACGACAAGGTCTACAAAAGCATTCTGGCGATGCTCGGGCATTTTCATTCCTCAAAATTCTTGAGTTGATGCTACAAACGTCAAGGCCACCAGTAATGTTATTCATGGAAAACGTTGTTGGATTTGAGACATCTGACACACATACAAAAATGATTGAGATATTGGAAAAAGCTGATTTTTCTAGACAGGAGTTTATTTTAAGCCCAATGCAATTTGGTGTTCCATATTCTAGGCCACGATACTTCTGCCTGGCTAAAAGGAGGCCTTTATCCTTTCACAGGCTATTATACAATAATCAACTTCTGTGGTCTCCAAGCCCATTACTTGAGAATGATGTTGACATAGAAACCATTGAAAATCATGGATCACAAGCGACTTCTGATAAGTTGCTGCTCTCTTGTGAACCAATATGTAGGTTTCTTGATCATTCCAGCCATCACAGTGAATTTAGTTTTTCTGATGCCAGTAGTATTCAAAGAGAGGGTGCATTGGAGAAAACCAAGGATACAGATGAGCAAGGATCTTTTGATCAGTATCTCGTCCCATCCAGTTTGATAGACAGATGGGGCAGTGCTATGGATATTGTGTATCCTGATTCAAGGCGATGCTGCTGTTTCACAAAAAGCTACTACCGATACGTGAAGGGTACTGGTTCACTTTTGGCACCTTTTGAGGAAGAAAAGATGGGCAAGGCTCATTCTCTGAAAGAACAGAAGCTCAGATATTTTACTCCTAGAGAGGTTGCTAACTTGCATTCCTTTCCGGAAGATTTTCAATTTCCACAGCATATCGGTCTTCGGCAACGGTAT

Protein sequence

MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRPYQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRPPVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPLSFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEFSFSDASSIQREGALEKTKDTDEQGSFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTKSYYRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQRY

Homology

[Close]

BLAST of MS001295 vs. NCBI nr
Match: XP_022132298.1 (DNA (cytosine-5)-methyltransferase isoform X1 [Momordica charantia])

HSP 1 Score: 742.3 bits (1915), Expect = 2.0e-210
Identity = 359/360 (99.72%), Postives = 359/360 (99.72%), Query Frame = 0


Query: 1   MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL 180

Query: 181 SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF 240
           SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF
Sbjct: 181 SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF 240

Query: 241 SFSDASSIQREGALEKTKDTDEQGSFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTKSY 300
           SFSDASSIQREGALEKTKDTDEQGSFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTKSY
Sbjct: 241 SFSDASSIQREGALEKTKDTDEQGSFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTKSY 300

Query: 301 YRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQRY 360
           YRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQ Y
Sbjct: 301 YRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQCY 360

[Close]

BLAST of MS001295 vs. NCBI nr
Match: XP_022961760.1 (tRNA (cytosine(38)-C(5))-methyltransferase [Cucurbita moschata])

HSP 1 Score: 674.9 bits (1740), Expect = 3.9e-190
Identity = 327/362 (90.33%), Postives = 339/362 (93.65%), Query Frame = 0


Query: 1   MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVSP+LQGEPWRVLEFYSGIGGMRYSLL AG+DAKV+QAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVSPKLQGEPWRVLEFYSGIGGMRYSLLRAGIDAKVIQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQK SGDARAFSFLKILELMLQ SRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMLQASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKA FSRQEFILSPMQF VPYSRPRYFCLAKRRPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKAGFSRQEFILSPMQFRVPYSRPRYFCLAKRRPL 180

Query: 181 SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF 240
           SF   L NNQLLWSP PLLEND D++ IE+HGSQ TSDK LLSCEPIC+FLD+S+H +EF
Sbjct: 181 SFQTQLCNNQLLWSPWPLLENDADLDIIESHGSQTTSDKFLLSCEPICKFLDNSNHQNEF 240

Query: 241 SFSDASSIQREGALEKTKDTDEQG--SFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK 300
           S  DASSI REGALEKTKD DEQ   SFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK
Sbjct: 241 SSHDASSIHREGALEKTKDVDEQDIESFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK 300

Query: 301 SYYRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQ 360
           SYYRYVKGTGSLLAPFE E+MGKAHSLKEQKLRYFTPREVANLHSFPEDF+FPQHIGLRQ
Sbjct: 301 SYYRYVKGTGSLLAPFEVERMGKAHSLKEQKLRYFTPREVANLHSFPEDFEFPQHIGLRQ 360

[Close]

BLAST of MS001295 vs. NCBI nr
Match: XP_038884640.1 (tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 670.6 bits (1729), Expect = 7.4e-189
Identity = 326/362 (90.06%), Postives = 340/362 (93.92%), Query Frame = 0


Query: 1   MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKV P+LQ EPWRVLEFYSGIGGMRYSLLSAG+ AKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVLPKLQEEPWRVLEFYSGIGGMRYSLLSAGIAAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP 120
           YQGNIESLTAADIDS+ AHVWLLSPPCQPYTRQGLQK SGDARAFSFLKILELMLQ SRP
Sbjct: 61  YQGNIESLTAADIDSHHAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMLQASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKA FSRQEFILSPMQFGVPYSRPRYFCLAKR+PL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKAGFSRQEFILSPMQFGVPYSRPRYFCLAKRKPL 180

Query: 181 SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF 240
           SF + LYN+QLLWSPSPLLEND +IE IENHGSQATSDKLLLSCEPICRFLD+S+HH+E 
Sbjct: 181 SFQKQLYNSQLLWSPSPLLENDANIEIIENHGSQATSDKLLLSCEPICRFLDNSNHHNEL 240

Query: 241 SFSDASSIQREGALEKTKDTDEQG--SFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK 300
           SF DASSI RE ALEK KD DEQ   S +QY+VPSSLIDRWGSAMDIVYPDSRRCCCFTK
Sbjct: 241 SFHDASSIPREVALEKDKDLDEQDLESLNQYVVPSSLIDRWGSAMDIVYPDSRRCCCFTK 300

Query: 301 SYYRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQ 360
           SYYRYVKGTGSLLAPFE E+MGKAHSLKEQKLRYFTPREVANLHSFPEDFQFP+HIGLRQ
Sbjct: 301 SYYRYVKGTGSLLAPFEAERMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPEHIGLRQ 360

[Close]

BLAST of MS001295 vs. NCBI nr
Match: KAG6598586.1 (tRNA (cytosine(38)-C(5))-methyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029520.1 tRNA (cytosine(38)-C(5))-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 669.1 bits (1725), Expect = 2.2e-188
Identity = 325/362 (89.78%), Postives = 339/362 (93.65%), Query Frame = 0


Query: 1   MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVSP+LQGEPWRVLEFYSGIGGMRYSLL AG+DAKV++AFDIN+KANDVYEHNFGDRP
Sbjct: 1   MEKVSPKLQGEPWRVLEFYSGIGGMRYSLLRAGIDAKVIRAFDINNKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQK SGDARAFSFLKILELMLQ SRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMLQASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKA FSRQEFILSPMQF VPYSRPRYFCLAKRRPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKAGFSRQEFILSPMQFRVPYSRPRYFCLAKRRPL 180

Query: 181 SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF 240
           SF   L NNQLLWSP PLLEND D+E IE+HGSQ TSDKLLLSCEPI +FLD+S+H +EF
Sbjct: 181 SFQTQLCNNQLLWSPRPLLENDADLEIIESHGSQTTSDKLLLSCEPIRKFLDNSNHQNEF 240

Query: 241 SFSDASSIQREGALEKTKDTDEQG--SFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK 300
           +  DASSI REGALEKTKD DEQ   SFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK
Sbjct: 241 TSHDASSIHREGALEKTKDVDEQDIESFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK 300

Query: 301 SYYRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQ 360
           SYYRYVKGTGSLLAPFE E+MGKAHSLKEQKLRYFTPREVANLHSFPEDF+FPQHIGLRQ
Sbjct: 301 SYYRYVKGTGSLLAPFEVERMGKAHSLKEQKLRYFTPREVANLHSFPEDFEFPQHIGLRQ 360

[Close]

BLAST of MS001295 vs. NCBI nr
Match: XP_023546512.1 (tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 668.7 bits (1724), Expect = 2.8e-188
Identity = 325/362 (89.78%), Postives = 338/362 (93.37%), Query Frame = 0


Query: 1   MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVSP+LQGEPWRVLEFYSGIGGMRYSLL AG+DAKV++AFDIN+KANDVYEHNFGDRP
Sbjct: 1   MEKVSPKLQGEPWRVLEFYSGIGGMRYSLLRAGIDAKVIRAFDINNKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQK SGDARAFSFLKILELMLQ SRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMLQASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKA FSRQEFILSPMQF VPYSRPRYFCLAKRRPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKAGFSRQEFILSPMQFRVPYSRPRYFCLAKRRPL 180

Query: 181 SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF 240
           SF   L NNQLLWSP PLLEN  D+E IE+HGSQ TSDKLLLSCEPIC+FLD+S+H +EF
Sbjct: 181 SFQTQLCNNQLLWSPRPLLENYADLEIIESHGSQTTSDKLLLSCEPICKFLDNSNHQNEF 240

Query: 241 SFSDASSIQREGALEKTKDTDEQ--GSFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK 300
           S  DASSI REGALEKTKD DEQ   SF QYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK
Sbjct: 241 SSHDASSIHREGALEKTKDVDEQDIDSFGQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK 300

Query: 301 SYYRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQ 360
           SYYRYVKGTGSLLAPFE E+MGKAHSLKEQKLRYFTPREVANLHSFPEDF+FPQHIGLRQ
Sbjct: 301 SYYRYVKGTGSLLAPFEVERMGKAHSLKEQKLRYFTPREVANLHSFPEDFEFPQHIGLRQ 360

[Close]

BLAST of MS001295 vs. ExPASy Swiss-Prot
Match: F4JWT7 (tRNA (cytosine(38)-C(5))-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=DNMT2 PE=1 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 5.6e-131
Identity = 231/351 (65.81%), Postives = 274/351 (78.06%), Query Frame = 0


Query: 11  EPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRPYQGNIESLTA 70
           +PW+VLEFYSGIGGMRYSL+++G+ ++VV+AF+IND ANDVY+HNF  RPYQGNI+SLTA
Sbjct: 13  KPWQVLEFYSGIGGMRYSLMASGIVSEVVEAFEINDSANDVYQHNFKHRPYQGNIQSLTA 72

Query: 71  ADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRPPVMLFMENVV 130
           AD+D Y A  WLLSPPCQPYTRQGLQKHSGDARAFSFL+ILEL+  T++PP MLF+ENVV
Sbjct: 73  ADLDKYNADAWLLSPPCQPYTRQGLQKHSGDARAFSFLRILELIPHTTKPPQMLFVENVV 132

Query: 131 GFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPLSFHRLLYNNQ 190
           GFETSDTH +MI  L K D+  QEFILSP+QFGVPYSRPRYFCLAKR+PL F     NN+
Sbjct: 133 GFETSDTHMEMIGTLTKLDYVTQEFILSPLQFGVPYSRPRYFCLAKRKPLPFKSQHSNNK 192

Query: 191 LLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSH-HSEFSFSDASSIQ 250
           LLWSP PL   D  +E  +    +   DKLL  C+P+ +FL+ ++H   E S  D S   
Sbjct: 193 LLWSPDPLYGRDDQVEFGKCQAEEGL-DKLLEFCKPVEKFLELAAHVDGEPSSVDDSENG 252

Query: 251 REGALEKTKDTDEQGSFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTKSYYRYVKGTGS 310
            +    +  D+    S  QYLVP SLI+RWG+AMDIVYPDS+RCCCFTKSYYRYVKGTGS
Sbjct: 253 SKDCCGQEGDS-VPDSVHQYLVPVSLIERWGNAMDIVYPDSKRCCCFTKSYYRYVKGTGS 312

Query: 311 LLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQRY 361
           LLA  + +  GK   LKEQ+LRYFTPREVAN HSFPEDF+FP+HI LRQRY
Sbjct: 313 LLATVQPKIKGKESCLKEQRLRYFTPREVANFHSFPEDFEFPKHISLRQRY 361

[Close]

BLAST of MS001295 vs. ExPASy Swiss-Prot
Match: O55055 (tRNA (cytosine(38)-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Trdmt1 PE=1 SV=2)

HSP 1 Score: 225.3 bits (573), Expect = 1.1e-57
Identity = 143/382 (37.43%), Postives = 198/382 (51.83%), Query Frame = 0


Query: 11  EPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNF-GDRPYQGNIESLT 70
           EP RVLE YSGIGGM ++L  + + A VV A D+N  AN+VY+HNF         IE ++
Sbjct: 2   EPLRVLELYSGIGGMHHALRESHIPAHVVAAIDVNTVANEVYKHNFPHTHLLSKTIEGIS 61

Query: 71  AADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRPPVMLFMENV 130
             D D    ++ L+SPPCQP+TR GLQ    D R  SFL IL+++ +  + P  + +ENV
Sbjct: 62  LEDFDKLSFNMILMSPPCQPFTRIGLQGDMTDPRTTSFLYILDILPRLQKLPKYILLENV 121

Query: 131 VGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPLSFHRLLYNN 190
            GFE S T   +I+ +E   F  QEF+LSP   G+P SR RYF +AK +   F       
Sbjct: 122 KGFEVSSTRGLLIQTIEACGFQYQEFLLSPSSLGIPNSRLRYFLIAKLQSEPFP------ 181

Query: 191 QLLWSPSPLLENDVDIETIENHG---SQATSDKLLLSCEPICRFLDHSSHHSE------F 250
               +P  +L     I T+E       + +  ++  +   IC   + SS  S       F
Sbjct: 182 --FQAPGQILMEFPKIVTVEPQKYAVVEESQPRVQRTGPRIC--AESSSTQSSGKDTILF 241

Query: 251 SFSDASSIQREG------ALEKTKDTDEQGSFDQYLVPSSLIDRWGSAMDIVYPDSRRCC 310
                    R+       +++  KD  E G  D+YL+P  L+ R+   +DIV P SRR  
Sbjct: 242 KLETVEERDRKHQQDSDLSVQMLKDFLEDGDTDEYLLPPKLLLRYALLLDIVKPTSRRSM 301

Query: 311 CFTKSYYRYVKGTGSLLAPFE----------------EEKMGKAHSLKEQKLRYFTPREV 361
           CFTK Y  Y++GTGS+L   E                EEK+ K   L   KLRYFTP+E+
Sbjct: 302 CFTKGYGSYIEGTGSVLQAAEDAQIENIYKSLPDLPPEEKIAKLSML---KLRYFTPKEI 361

[Close]

BLAST of MS001295 vs. ExPASy Swiss-Prot
Match: Q54JH6 (DNA (cytosine-5)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=dnmA PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 7.0e-57
Identity = 130/355 (36.62%), Postives = 193/355 (54.37%), Query Frame = 0


Query: 11  EPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRPYQGNIESLTA 70
           E  RVLEFYSGIGGM Y L  +GVD +V+Q+FDIN  AN  Y++ F +   Q +IES + 
Sbjct: 2   EQLRVLEFYSGIGGMHYGLQESGVDFQVIQSFDINTNANLNYKYTFNEDSSQKSIESYSV 61

Query: 71  ADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRPPVMLFMENVV 130
            +++ ++A+ WL+SPPCQP+TR GLQK   D R  SF  +L+++ +   PP  + +ENV 
Sbjct: 62  EELEGFKANAWLMSPPCQPFTRLGLQKDDQDNRTNSFFHLLDVLTKIKDPPTYILIENVF 121

Query: 131 GFE---TSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPLSFHRLLY 190
           GF    +S+T   +++ L K ++S QEF LSP QFG+   R RYFC+AKR          
Sbjct: 122 GFAKKGSSNTRDHLLDTLIKMNYSFQEFHLSPQQFGLANQRLRYFCIAKR---------- 181

Query: 191 NNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEFSFSDASS 250
           N +L +       N+   E   N+ S   +++       I   +     H+     D  S
Sbjct: 182 NGKLNFKKEQDKHNEKVDENKLNNNSNNNNEQNKYDNLKILDHIPGYDFHTTLEECDEIS 241

Query: 251 IQREGALEKTKDTDEQGSFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTKSYYRYVKGT 310
              +      KD  +   +++Y VP +L+   G   DI   DS+   C TKSY ++++GT
Sbjct: 242 NYFD------KDLTDDELYEKYKVPHNLLLSKGMLFDIKQKDSKTSNCVTKSYGKFIEGT 301

Query: 311 GSLLAPFEEEK--MGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQRY 361
           GS++      K  +    SL   KLRYF+P+E+  LH FPE+F+F   +   Q Y
Sbjct: 302 GSIIQMDNNFKADINDNKSLIPLKLRYFSPKEITRLHGFPEEFKFSPKLTTIQCY 340

[Close]

BLAST of MS001295 vs. ExPASy Swiss-Prot
Match: Q4G073 (tRNA (cytosine(38)-C(5))-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trdmt1 PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.7e-56
Identity = 143/382 (37.43%), Postives = 197/382 (51.57%), Query Frame = 0


Query: 11  EPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRPYQG-NIESLT 70
           EP RVLE YSGIGGM ++L  + V A VV A D++  AN+VY+HNF         IE ++
Sbjct: 2   EPLRVLELYSGIGGMHHALRESRVPAHVVAAIDVSTVANEVYKHNFPHTHLLAKTIEGIS 61

Query: 71  AADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRPPVMLFMENV 130
             + D    ++ L+SPPCQP+TR GLQ    D R  SFL IL+++ +  + P  + +ENV
Sbjct: 62  LEEFDKLSFNMILMSPPCQPFTRIGLQGDMSDRRTNSFLYILDILPRLQKLPKYILLENV 121

Query: 131 VGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAK--RRPLSFHRLLY 190
            GFE S T   +I+ +E   F  QEF+LSP   G+P SR RYF +AK    PL F     
Sbjct: 122 KGFEVSSTRGLLIQTMEACGFQYQEFLLSPSSLGIPNSRLRYFLIAKLQSEPLCFQ---- 181

Query: 191 NNQLLWSPSPLLENDVDIETIENHGSQATSD-KLLLSCEPICRFLDHSSHHSE------F 250
                 +P  +L    +  T++        + KL +        LD SS          F
Sbjct: 182 ------APGQILMEFPNSGTVQPQEYAVVEEGKLRVRTREPDVCLDSSSTQCSGQDSILF 241

Query: 251 SFSDASSIQREG------ALEKTKDTDEQGSFDQYLVPSSLIDRWGSAMDIVYPDSRRCC 310
               A+ I R+       +++  K   E G   QYL+P+  + R+   +DIV P SRR  
Sbjct: 242 KHETAADIDRKRQQDSDLSVQMLKGFLEDGDTAQYLLPAKSLLRYALLLDIVKPTSRRSM 301

Query: 311 CFTKSYYRYVKGTGSLLAPFE----------------EEKMGKAHSLKEQKLRYFTPREV 361
           CFTK Y  Y++GTGS+L   E                EEK+ K   L   KLRYFTP+E+
Sbjct: 302 CFTKGYGSYIEGTGSVLQTAEDVQIENIYKSLPDLPPEEKIAKLSML---KLRYFTPKEI 361

[Close]

BLAST of MS001295 vs. ExPASy Swiss-Prot
Match: O14717 (tRNA (cytosine(38)-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=TRDMT1 PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.7e-55
Identity = 144/380 (37.89%), Postives = 202/380 (53.16%), Query Frame = 0


Query: 11  EPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRPYQG-NIESLT 70
           EP RVLE YSG+GGM ++L  + + A+VV A D+N  AN+VY++NF         IE +T
Sbjct: 2   EPLRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNFPHTQLLAKTIEGIT 61

Query: 71  AADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRPPVMLFMENV 130
             + D     + L+SPPCQP+TR G Q    D+R  SFL IL+++ +  + P  + +ENV
Sbjct: 62  LEEFDRLSFDMILMSPPCQPFTRIGRQGDMTDSRTNSFLHILDILPRLQKLPKYILLENV 121

Query: 131 VGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAK--RRPLSFH---R 190
            GFE S T   +I+ +E   F  QEF+LSP   G+P SR RYF +AK    PL F    +
Sbjct: 122 KGFEVSSTRDLLIQTIENCGFQYQEFLLSPTSLGIPNSRLRYFLIAKLQSEPLPFQAPGQ 181

Query: 191 LLYNNQLLWSPSPL-----LENDVDIETIENH----GSQATS--DKLLLSCEPICRFLDH 250
           +L     + S  P      +EN +  + +E +    GS   S  D +L   E        
Sbjct: 182 VLMEFPKIESVHPQKYAMDVENKIQEKNVEPNISFDGSIQCSGKDAILFKLETA-----E 241

Query: 251 SSHHSEFSFSDASSIQREGALEKTKDTDEQGSFDQYLVPSSLIDRWGSAMDIVYPDSRRC 310
             H      SD S    +  LE   DTD     +QYL+P   + R+   +DIV P  RR 
Sbjct: 242 EIHRKNQQDSDLSVKMLKDFLE--DDTD----VNQYLLPPKSLLRYALLLDIVQPTCRRS 301

Query: 311 CCFTKSYYRYVKGTGSLLAPFEEEKMGKAH------SLKEQ-------KLRYFTPREVAN 361
            CFTK Y  Y++GTGS+L   E+ ++   +      S +EQ       KLRYFTP+E+AN
Sbjct: 302 VCFTKGYGSYIEGTGSVLQTAEDVQVENIYKSLTNLSQEEQITKLLILKLRYFTPKEIAN 361

[Close]

BLAST of MS001295 vs. ExPASy TrEMBL
Match: A0A6J1BVV7 (DNA (Cytosine-5)-methyltransferase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005184 PE=3 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 9.7e-211
Identity = 359/360 (99.72%), Postives = 359/360 (99.72%), Query Frame = 0


Query: 1   MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL 180

Query: 181 SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF 240
           SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF
Sbjct: 181 SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF 240

Query: 241 SFSDASSIQREGALEKTKDTDEQGSFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTKSY 300
           SFSDASSIQREGALEKTKDTDEQGSFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTKSY
Sbjct: 241 SFSDASSIQREGALEKTKDTDEQGSFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTKSY 300

Query: 301 YRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQRY 360
           YRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQ Y
Sbjct: 301 YRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQCY 360

[Close]

BLAST of MS001295 vs. ExPASy TrEMBL
Match: A0A6J1HD73 (tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111462432 PE=3 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 1.9e-190
Identity = 327/362 (90.33%), Postives = 339/362 (93.65%), Query Frame = 0


Query: 1   MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVSP+LQGEPWRVLEFYSGIGGMRYSLL AG+DAKV+QAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVSPKLQGEPWRVLEFYSGIGGMRYSLLRAGIDAKVIQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQK SGDARAFSFLKILELMLQ SRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMLQASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKA FSRQEFILSPMQF VPYSRPRYFCLAKRRPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKAGFSRQEFILSPMQFRVPYSRPRYFCLAKRRPL 180

Query: 181 SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF 240
           SF   L NNQLLWSP PLLEND D++ IE+HGSQ TSDK LLSCEPIC+FLD+S+H +EF
Sbjct: 181 SFQTQLCNNQLLWSPWPLLENDADLDIIESHGSQTTSDKFLLSCEPICKFLDNSNHQNEF 240

Query: 241 SFSDASSIQREGALEKTKDTDEQG--SFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK 300
           S  DASSI REGALEKTKD DEQ   SFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK
Sbjct: 241 SSHDASSIHREGALEKTKDVDEQDIESFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK 300

Query: 301 SYYRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQ 360
           SYYRYVKGTGSLLAPFE E+MGKAHSLKEQKLRYFTPREVANLHSFPEDF+FPQHIGLRQ
Sbjct: 301 SYYRYVKGTGSLLAPFEVERMGKAHSLKEQKLRYFTPREVANLHSFPEDFEFPQHIGLRQ 360

[Close]

BLAST of MS001295 vs. ExPASy TrEMBL
Match: A0A6J1K9S0 (tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111491952 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 3.1e-188
Identity = 326/363 (89.81%), Postives = 339/363 (93.39%), Query Frame = 0


Query: 1   MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKVSP+LQGEPWRVLEFYSGIGGMRYSLL AG+DAKV+QAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVSPKLQGEPWRVLEFYSGIGGMRYSLLRAGIDAKVIQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP 120
           YQGNIESLTAADIDSY+AHVWLLSPPCQPYTRQGLQK SGDARAFSFLKILELMLQ SRP
Sbjct: 61  YQGNIESLTAADIDSYRAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMLQASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKA FSRQEFILSPMQF VPYSRPRYFCLAKRRPL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKAGFSRQEFILSPMQFRVPYSRPRYFCLAKRRPL 180

Query: 181 SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF 240
           SF   L NNQLLWSP PLLEND D+E IE+HGSQ TSDKLLLSCEPIC+FLD+S+H +EF
Sbjct: 181 SFQTQLCNNQLLWSPWPLLENDADLEIIESHGSQTTSDKLLLSCEPICKFLDNSNHQNEF 240

Query: 241 SFSDASSIQR-EGALEKTKDTDEQG--SFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFT 300
           S  DASSI R EGALEK KD DEQ   SFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFT
Sbjct: 241 SSHDASSIHREEGALEKIKDVDEQDIESFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFT 300

Query: 301 KSYYRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLR 360
           KSYYRYVKGTGSLLAPFE ++MGKAHSLKEQKLRYFTPREVANLHSFPEDF+FPQHIGLR
Sbjct: 301 KSYYRYVKGTGSLLAPFEVKRMGKAHSLKEQKLRYFTPREVANLHSFPEDFEFPQHIGLR 360

[Close]

BLAST of MS001295 vs. ExPASy TrEMBL
Match: A0A5A7VCE7 (tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002740 PE=3 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 3.4e-187
Identity = 324/362 (89.50%), Postives = 338/362 (93.37%), Query Frame = 0


Query: 1   MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKV P+LQ EPWRVLEFYSGIGGMRYSLLSAG+ AKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQK SGDARAFSFLKILELMLQ SRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMLQASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKA FS QEFILSPMQFGVPYSRPRYFCLAKR+PL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKAGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180

Query: 181 SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF 240
           SF + L+NNQLLWSPSPLL+ND D+E+IE HGSQATSDKLLLSCEPICRFLDHS+H  E 
Sbjct: 181 SFQKQLHNNQLLWSPSPLLDNDADLESIETHGSQATSDKLLLSCEPICRFLDHSNHR-EL 240

Query: 241 SFSDASSIQREGALEKTKDTDEQ--GSFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK 300
           SF DASSIQ E ALEK +D +EQ   SF+QYLVPS LIDRWGSAMDIVYPDSRRCCCFTK
Sbjct: 241 SFYDASSIQSEVALEKNEDPNEQVIESFNQYLVPSGLIDRWGSAMDIVYPDSRRCCCFTK 300

Query: 301 SYYRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQ 360
           SYYRYVKGTGSLLAPFE E+MGKAHSLKEQKLRYFTPREVANLHSFP+DFQFPQHIGLRQ
Sbjct: 301 SYYRYVKGTGSLLAPFEAERMGKAHSLKEQKLRYFTPREVANLHSFPKDFQFPQHIGLRQ 360

[Close]

BLAST of MS001295 vs. ExPASy TrEMBL
Match: A0A1S3BC07 (tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488282 PE=3 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 3.4e-187
Identity = 324/362 (89.50%), Postives = 338/362 (93.37%), Query Frame = 0


Query: 1   MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60
           MEKV P+LQ EPWRVLEFYSGIGGMRYSLLSAG+ AKVVQAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRP 120
           YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQK SGDARAFSFLKILELMLQ SRP
Sbjct: 61  YQGNIESLTAADIDSYQAHVWLLSPPCQPYTRQGLQKQSGDARAFSFLKILELMLQASRP 120

Query: 121 PVMLFMENVVGFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPL 180
           PVMLFMENVVGFETSDTHTKMIEILEKA FS QEFILSPMQFGVPYSRPRYFCLAKR+PL
Sbjct: 121 PVMLFMENVVGFETSDTHTKMIEILEKAGFSTQEFILSPMQFGVPYSRPRYFCLAKRKPL 180

Query: 181 SFHRLLYNNQLLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSHHSEF 240
           SF + L+NNQLLWSPSPLL+ND D+E+IE HGSQATSDKLLLSCEPICRFLDHS+H  E 
Sbjct: 181 SFQKQLHNNQLLWSPSPLLDNDADLESIETHGSQATSDKLLLSCEPICRFLDHSNHR-EL 240

Query: 241 SFSDASSIQREGALEKTKDTDEQ--GSFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTK 300
           SF DASSIQ E ALEK +D +EQ   SF+QYLVPS LIDRWGSAMDIVYPDSRRCCCFTK
Sbjct: 241 SFYDASSIQSEVALEKNEDPNEQVIESFNQYLVPSGLIDRWGSAMDIVYPDSRRCCCFTK 300

Query: 301 SYYRYVKGTGSLLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQ 360
           SYYRYVKGTGSLLAPFE E+MGKAHSLKEQKLRYFTPREVANLHSFP+DFQFPQHIGLRQ
Sbjct: 301 SYYRYVKGTGSLLAPFEAERMGKAHSLKEQKLRYFTPREVANLHSFPKDFQFPQHIGLRQ 360

[Close]

BLAST of MS001295 vs. TAIR 10
Match: AT5G25480.1 (DNA methyltransferase-2 )

HSP 1 Score: 468.8 bits (1205), Expect = 4.0e-132
Identity = 231/351 (65.81%), Postives = 274/351 (78.06%), Query Frame = 0


Query: 11  EPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRPYQGNIESLTA 70
           +PW+VLEFYSGIGGMRYSL+++G+ ++VV+AF+IND ANDVY+HNF  RPYQGNI+SLTA
Sbjct: 13  KPWQVLEFYSGIGGMRYSLMASGIVSEVVEAFEINDSANDVYQHNFKHRPYQGNIQSLTA 72

Query: 71  ADIDSYQAHVWLLSPPCQPYTRQGLQKHSGDARAFSFLKILELMLQTSRPPVMLFMENVV 130
           AD+D Y A  WLLSPPCQPYTRQGLQKHSGDARAFSFL+ILEL+  T++PP MLF+ENVV
Sbjct: 73  ADLDKYNADAWLLSPPCQPYTRQGLQKHSGDARAFSFLRILELIPHTTKPPQMLFVENVV 132

Query: 131 GFETSDTHTKMIEILEKADFSRQEFILSPMQFGVPYSRPRYFCLAKRRPLSFHRLLYNNQ 190
           GFETSDTH +MI  L K D+  QEFILSP+QFGVPYSRPRYFCLAKR+PL F     NN+
Sbjct: 133 GFETSDTHMEMIGTLTKLDYVTQEFILSPLQFGVPYSRPRYFCLAKRKPLPFKSQHSNNK 192

Query: 191 LLWSPSPLLENDVDIETIENHGSQATSDKLLLSCEPICRFLDHSSH-HSEFSFSDASSIQ 250
           LLWSP PL   D  +E  +    +   DKLL  C+P+ +FL+ ++H   E S  D S   
Sbjct: 193 LLWSPDPLYGRDDQVEFGKCQAEEGL-DKLLEFCKPVEKFLELAAHVDGEPSSVDDSENG 252

Query: 251 REGALEKTKDTDEQGSFDQYLVPSSLIDRWGSAMDIVYPDSRRCCCFTKSYYRYVKGTGS 310
            +    +  D+    S  QYLVP SLI+RWG+AMDIVYPDS+RCCCFTKSYYRYVKGTGS
Sbjct: 253 SKDCCGQEGDS-VPDSVHQYLVPVSLIERWGNAMDIVYPDSKRCCCFTKSYYRYVKGTGS 312

Query: 311 LLAPFEEEKMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHIGLRQRY 361
           LLA  + +  GK   LKEQ+LRYFTPREVAN HSFPEDF+FP+HI LRQRY
Sbjct: 313 LLATVQPKIKGKESCLKEQRLRYFTPREVANFHSFPEDFEFPKHISLRQRY 361

The following BLAST results are available for this feature:

BLAST of MS001295 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132298.1	2.0e-210	99.72	DNA (cytosine-5)-methyltransferase isoform X1 [Momordica charantia]	[more]
XP_022961760.1	3.9e-190	90.33	tRNA (cytosine(38)-C(5))-methyltransferase [Cucurbita moschata]	[more]
XP_038884640.1	7.4e-189	90.06	tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X1 [Benincasa hispida]	[more]
KAG6598586.1	2.2e-188	89.78	tRNA (cytosine(38)-C(5))-methyltransferase 2, partial [Cucurbita argyrosperma su...	[more]
XP_023546512.1	2.8e-188	89.78	tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucurbita pepo subsp. pep...	[more]

BLAST of MS001295 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JWT7	5.6e-131	65.81	tRNA (cytosine(38)-C(5))-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O55055	1.1e-57	37.43	tRNA (cytosine(38)-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Trdmt1 PE...	[more]
Q54JH6	7.0e-57	36.62	DNA (cytosine-5)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=dnmA ...	[more]
Q4G073	2.7e-56	37.43	tRNA (cytosine(38)-C(5))-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trdm...	[more]
O14717	1.7e-55	37.89	tRNA (cytosine(38)-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=TRDMT1 PE=...	[more]

BLAST of MS001295 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVV7	9.7e-211	99.72	DNA (Cytosine-5)-methyltransferase isoform X1 OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1HD73	1.9e-190	90.33	tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1K9S0	3.1e-188	89.81	tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A5A7VCE7	3.4e-187	89.50	tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo var. makuw...	[more]
A0A1S3BC07	3.4e-187	89.50	tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN...	[more]

BLAST of MS001295 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25480.1	4.0e-132	65.81	DNA methyltransferase-2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001525	C-5 cytosine methyltransferase	PRINTS	PR00105	C5METTRFRASE	coord: 14..30 score: 32.88 coord: 120..134 score: 36.2 coord: 163..176 score: 38.64
IPR001525	C-5 cytosine methyltransferase	PFAM	PF00145	DNA_methylase	coord: 14..353 e-value: 3.7E-32 score: 112.0
IPR001525	C-5 cytosine methyltransferase	PROSITE	PS51679	SAM_MT_C5	coord: 13..360 score: 35.886425
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 6..190 e-value: 3.7E-47 score: 162.0
None	No IPR available	GENE3D	3.90.120.10	DNA Methylase, subunit A, domain 2	coord: 243..360 e-value: 3.0E-34 score: 119.9
None	No IPR available	PANTHER	PTHR46098	TRNA (CYTOSINE(38)-C(5))-METHYLTRANSFERASE	coord: 11..360
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 12..360

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001295.1	MS001295.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008168	methyltransferase activity