MS001286 (gene) Bitter gourd (TR) v1

Overview


Name	MS001286
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	N-alpha-acetyltransferase 50
Location	scaffold36: 2978667 .. 2982662 (+)
RNA-Seq Expression	MS001286
Synteny	MS001286

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGGCGGGGCGCGAAGTCCCAATTTCGCTCGACGGTGTGAGGGACAAGAACTTGATGCAGCTCAAGAAGCTCAACACAGCTCTCTTCCCTGTTCGCTACAACGAAAAGTACTACGTCGATGTCCTTGCCTCTGGCGAGTTCACTAAGCTAGGTCTGATTACTTCTTCTATCGACTCTACGGCCTGTATCTTCTTCTCTTCTCCATTCTTTTGATTCTCTCATCATTGGTTCTCGATTTTTACCTTTTGAGCATTCTTATTGTGTTCTTTATCATTCTGCCTTTCGTCCTTTGCTTATGAATCAGCCGATTTTCTTTACGGATTTAGCTACTAGTTATTTGGGAGGTGGGAAGAATGTTCGTAGGGTTTCTACTATGAATTATGTGGAGTTAGTTCAGTTCTTTGGTGGGTTTTGAAGTATTAAGCTGTTTGAACTTTGGAATTGGCATTATGTTTTTCTGCTTGGATGCTCTTTGAGACATTATATGTAGTATATCTCGATTTTATATTCATTATTATTGGTAGCTTGCAGGCTTTAAGAGCCAGATTAGTGTTAATTACAAGAAAGGGATGGGAGAAAAAATAATGGGAACCGAGAAAACAGATTAGGTGGGCTTACCTTTCAAACTAAAGGCCTTTTTAGTTTATTCACAAATTTTAAACTAGTGCTTGATAATTCTTCAATTTTATCCACTATGACTTAATCTCTTCCAAAAATTTCCATTTGGTTTCTCCAGCTTCCTGGTAGGAATTATCAATCTAAACCTATATTATAGTTTGTTTGTTAGAAAAATGAGTCATCCACTGGCTACTGTTGGTAGGTTTCTCGGACCATGACTATGATTTTGATTGCTCTTGCGAGGCAATGAATTATTGTTGTTTTTATTTTGTGGAAATAGTTCGGCATTGAGTTATTTTTAACTGTGGGTTTGATCAATTTATTTATTTATAATATATCTCTTGCTTCTTTGGTCATGTGGAACCATCAAACGATTGGTTCGTAAGTTCAACAGTTGATGGTTCAAGATATTTAGTTGACTTGTTACTTGTACTATTTCTCATCATGGATCACTCAAGGTCCACTGGAGATCCATTTTTCAGTATTTTTTCATTGTGAAGAACTTGTTGGAATGAATCTTTCTTCTAGATAGTAGGAGTCATCGTTTTATGCTTTCACTACTACAGTTGAAGATCACCCTAGCAACTTGATATGGGTGCTTTCTGAAGTATCCTCCACTTTTTCTTATTTTTAGATTCATTCACAGTAAGTTTTTCTTGCATAAAACTGACCGTATAAAGTTTGTGTGAGAAACTAGGGATGCAATTCAAGCATGAACTTGAATTTTTGTAGTATCCAATCTATCTGTACACTATGTAACGTGAGATCCTTTTTTGTAATGACATGTAATGTTTTTATTCCTAGCTTCATAGTTTTTATCTTTCCGAAATAAATCTCTCTATAATTTGTAGATATGGTTTTCATGCGCTTCAGAATTAATGAAGTATGATTTTTTCACTTCACTCCTAGCTTGAAATAAGTTAAAATCTTCCTTGCCAATTCATCAACTTGTTTTCCCTTTAATTAGGAAAATTGAAATTGTGTTTGGGTGTTTCATGACTATGCTAGTTCTCAGGTGCACCGTGCACATACCACACTTATGCATTAAAGTTCTACTATTTTATTTTTAGGCTTTCATTTCCTTTCTACTAAAAGATAGGTAATTCATTTTCTTCATCTCTGATTTTAACCTTGCGTATTTAAGTCCCATCATTTTTTTAATAGGAAACAAAAGAATATATTAATGGAGAAACAGAAAGATCATACTACAGAAAGGGGAGATGGGATATCCCACAAGGTCACCATACTCCATAGGGTTACAAAAAAGATTTTCAATTGGCTGTAATATGAACAAGATCATAATTACAGACGGGTTTGGAAAGAGAATACCGAAGGGATGCAAAGAGTCTAACTGAGTCCCAGAAATCCTTAGACTAGGGTATCTCTCTTTGGTTTGTGTGTTTTCAATTTGCACGTATTGCACTAGTCTACTGAAAATTTGCGAGTTTCTGCTTTAAGAAAAAGGCTAGGAAAAAAAGAACAATCTAGTTTCCTTTTCTAAATTCTTCTCTTGTGCATTTCAGTAATTTGACGATTTAGTAGATTTAACCTGCTGCAGGACTACTCCGATGCATTTTAGAATGTGAATTTTGCATAATTCTAGCCATAAGATTATCCTGTTAGTTCTGAGTCATATACTGAAAATTAATCAGTATACTAATTAATAGTTACCACTTTGAATGCCTTATAGGTATTACAATGGTTTTTGTGAATTTAAAACAAAACCTTAATCTTGCTATTTTAATTGTGCTAATAGGTTGTATAGTTGTATTCCAAAAGGCTAAAACTGCGTATTCCTATTGGTTTTGCAGCATACTACAGTGATATATGTGTTGGTTCCATTGCATGCCGCCTTGAGAAGAAGGAAAATGGATCCGTACGTGTCTACATCATGACCCTGGGTGTTCTAGCACCGTATCGTGGATTAGGCATCGGTAAGTACCTACTTCATGTCTCCCATAAGACCTTTTGTTTGTATGTTTGTGCTTTTTTTTTTCCCGTTAAGAAATGGGGTACATTAAGTTACTAAGAGAGAGTAGAAAACCCTACTGGGGAAGGCTATTACAAAAAGTGTCTCCAATCCAAATTAATCATTGAAAAAATAAAAACTGTGATTACAAAAGAACTTCATGGGAAGGGCACTCCAACTTGAGGTCACATACCGTACAAGTTCACAGAAAGATTCAAAAGACTCCACAAGAAACCTTCTAAATACACACTTCCGTAGCACATTAGCTTCATCTTTCAGCCAACTGAAGAGGAGAATTTTGGGAAGCCAAAAATCAAGCTTATTCCGCATAACAGAGGAGATGCTAGAAATTTTAAGCAGAAAAAGACCATCCATTCTCATGCAGAAAAATGGCAGTGCAAACTGCAAAAGCCGAAAAGAGATGATCAAGAGTTTCTCTCTCTTCTAGACATGAATAGCAATCGTTTAGGGCAATGGGCCGGCTGGGATTTTTCTTTTAAAGCTTTTCTTACAGTGACAGTTTTGTGTCTAAGATGGATCCAACTTCCAATTTCTTTTATCAGAAGTATGTCCTCTAAGACCCAAATCCCAGTTATGACCCTCATCGTTACAACAATTGCCAATTACTGCAGCAAAAATAAGAGTTTGCATGGGAGTGGGGGAGGGCAATCAACATTCTTTTAAATTTGGAATTTTGGAGTACGAAGCCCATATTTGATTGTAATGAAATTCCTCCAAAGATCAAAAGCATTTTCCGCATTATTAAATCTCCACGTCCATTTAACTACGAGTGCCTTGTTTCCTTTCTTTCAAAGCCCTAATACCAATGCCATACAAAGAGAGATGAAGAACAGTCATTTCCATCTAACAAGATGGCTGCCCTGACCTTGGTTGGTACCCCTCTCTAGAAGAAGTTCCTTATGGTCTTCTCCAAACATTTTACTACCTTGGAAGGGATTTTATAATATTTTCTAGTAATGTTTGGGCATTGTGCCCTAGGTTATGGTATTTGCTAGTAACGGAAATAAGAAAAACTGAAGAAAAGTCACATGACGTGCAAAATTGAAGTATCATAGTTGGTTAAAAGGCCTTTATTTTATAATTCTTAAAAACCATTTGATTGAGATTAGAAGTTGATACTTTTTGGTGACACATTTTAGTTGCTTGTTTTATGATTTGGGTTTTATGCAGGTAGTAGACTGTTAAATCATGCTCTTGATCTCTGCTCAAAGCAAAACATTACTGAAATCTATTTGCATGTTCAGACGAATAATGATGATGCCATCAATTTCTATAAGAAATTTGGGTTTGAGATTACAGAAACCATTCAGAACTATTATGCAAACATTACCCCGCCAGATTGCTATGTTCTTACCAAGTTAATCACTCAAACTCAGACCAAGAAG

mRNA sequence

ATGGGGGCGGGGCGCGAAGTCCCAATTTCGCTCGACGGTGTGAGGGACAAGAACTTGATGCAGCTCAAGAAGCTCAACACAGCTCTCTTCCCTGTTCGCTACAACGAAAAGTACTACGTCGATGTCCTTGCCTCTGGCGAGTTCACTAAGCTAGCATACTACAGTGATATATGTGTTGGTTCCATTGCATGCCGCCTTGAGAAGAAGGAAAATGGATCCGTACGTGTCTACATCATGACCCTGGGTGTTCTAGCACCGTATCGTGGATTAGGCATCGGTAATAGACTGTTAAATCATGCTCTTGATCTCTGCTCAAAGCAAAACATTACTGAAATCTATTTGCATGTTCAGACGAATAATGATGATGCCATCAATTTCTATAAGAAATTTGGGTTTGAGATTACAGAAACCATTCAGAACTATTATGCAAACATTACCCCGCCAGATTGCTATGTTCTTACCAAGTTAATCACTCAAACTCAGACCAAGAAG

Coding sequence (CDS)

ATGGGGGCGGGGCGCGAAGTCCCAATTTCGCTCGACGGTGTGAGGGACAAGAACTTGATGCAGCTCAAGAAGCTCAACACAGCTCTCTTCCCTGTTCGCTACAACGAAAAGTACTACGTCGATGTCCTTGCCTCTGGCGAGTTCACTAAGCTAGCATACTACAGTGATATATGTGTTGGTTCCATTGCATGCCGCCTTGAGAAGAAGGAAAATGGATCCGTACGTGTCTACATCATGACCCTGGGTGTTCTAGCACCGTATCGTGGATTAGGCATCGGTAATAGACTGTTAAATCATGCTCTTGATCTCTGCTCAAAGCAAAACATTACTGAAATCTATTTGCATGTTCAGACGAATAATGATGATGCCATCAATTTCTATAAGAAATTTGGGTTTGAGATTACAGAAACCATTCAGAACTATTATGCAAACATTACCCCGCCAGATTGCTATGTTCTTACCAAGTTAATCACTCAAACTCAGACCAAGAAG

Protein sequence

MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVGSIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQNITEIYLHVQTNNDDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK

Homology

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BLAST of MS001286 vs. NCBI nr
Match: XP_022132323.1 (N-alpha-acetyltransferase 50 [Momordica charantia])

HSP 1 Score: 332.8 bits (852), Expect = 1.7e-87
Identity = 163/164 (99.39%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG 60
           MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG
Sbjct: 1   MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG 60

Query: 61  SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQNITEIYLHVQTNN 120
           SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIG+RLLNHALDLCSKQNITEIYLHVQTNN
Sbjct: 61  SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGSRLLNHALDLCSKQNITEIYLHVQTNN 120

Query: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 165
           DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK
Sbjct: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 164

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BLAST of MS001286 vs. NCBI nr
Match: XP_038884070.1 (N-alpha-acetyltransferase 50 [Benincasa hispida])

HSP 1 Score: 324.7 bits (831), Expect = 4.6e-85
Identity = 158/164 (96.34%), Postives = 160/164 (97.56%), Query Frame = 0


Query: 1   MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG 60
           MG GREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYY DVLASGEFTKLAYYSDICVG
Sbjct: 1   MGVGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYADVLASGEFTKLAYYSDICVG 60

Query: 61  SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQNITEIYLHVQTNN 120
           SIACRLEKKE+GSVRVYIMTLGVLAPYRGLGIG RLLNH LDLCSKQNITEIYLHVQTNN
Sbjct: 61  SIACRLEKKEHGSVRVYIMTLGVLAPYRGLGIGGRLLNHVLDLCSKQNITEIYLHVQTNN 120

Query: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 165
           DDAINFYKKFGFEITETIQNYYANITPPDCYVLT+LITQTQTKK
Sbjct: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTRLITQTQTKK 164

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BLAST of MS001286 vs. NCBI nr
Match: XP_004138740.1 (N-alpha-acetyltransferase 50 [Cucumis sativus] >XP_008445150.1 PREDICTED: N-alpha-acetyltransferase 50 [Cucumis melo] >KAA0064960.1 N-alpha-acetyltransferase 50 [Cucumis melo var. makuwa])

HSP 1 Score: 323.2 bits (827), Expect = 1.3e-84
Identity = 157/164 (95.73%), Postives = 160/164 (97.56%), Query Frame = 0


Query: 1   MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG 60
           MG GR+VPISLDGVRDKNLMQLKKLNTALFPVRYNEKYY DVLASGEFTKLAYYSDICVG
Sbjct: 1   MGVGRQVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYADVLASGEFTKLAYYSDICVG 60

Query: 61  SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQNITEIYLHVQTNN 120
           SIACRLEKKE+GSVRVYIMTLGVLAPYRGLGIG+RLLNH LDLCSKQNI EIYLHVQTNN
Sbjct: 61  SIACRLEKKEHGSVRVYIMTLGVLAPYRGLGIGSRLLNHVLDLCSKQNIAEIYLHVQTNN 120

Query: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 165
           DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK
Sbjct: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 164

[Close]

BLAST of MS001286 vs. NCBI nr
Match: XP_023545587.1 (N-alpha-acetyltransferase 50-like [Cucurbita pepo subsp. pepo] >KAG6598593.1 N-alpha-acetyltransferase 50, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029526.1 N-alpha-acetyltransferase 50 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 312.0 bits (798), Expect = 3.1e-81
Identity = 153/164 (93.29%), Postives = 157/164 (95.73%), Query Frame = 0


Query: 1   MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG 60
           M AGREVPISLD VRDKNLMQLKKLNTALFPVRYNEKYY DVLASG+FTKLAYYSDICVG
Sbjct: 1   MVAGREVPISLDEVRDKNLMQLKKLNTALFPVRYNEKYYADVLASGDFTKLAYYSDICVG 60

Query: 61  SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQNITEIYLHVQTNN 120
           SIACRLEKKE+GSVRVYIMTLGVLAPYRGLGIG++LLNH LDLCSKQNI EIYLHVQTNN
Sbjct: 61  SIACRLEKKEHGSVRVYIMTLGVLAPYRGLGIGSKLLNHVLDLCSKQNIPEIYLHVQTNN 120

Query: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 165
           DDAI FY KFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK
Sbjct: 121 DDAIKFYTKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 164

[Close]

BLAST of MS001286 vs. NCBI nr
Match: XP_022996667.1 (N-alpha-acetyltransferase 50-like [Cucurbita maxima])

HSP 1 Score: 310.8 bits (795), Expect = 6.8e-81
Identity = 152/164 (92.68%), Postives = 157/164 (95.73%), Query Frame = 0


Query: 1   MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG 60
           M AGREVPISLD VRDKNLMQLKKLNTALFPVRYNEKYY DVLASG+FTKLAYYSDICVG
Sbjct: 1   MVAGREVPISLDEVRDKNLMQLKKLNTALFPVRYNEKYYADVLASGDFTKLAYYSDICVG 60

Query: 61  SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQNITEIYLHVQTNN 120
           SIACRLEKK++GSVRVYIMTLGVLAPYRGLGIG++LLNH LDLCSKQNI EIYLHVQTNN
Sbjct: 61  SIACRLEKKDHGSVRVYIMTLGVLAPYRGLGIGSKLLNHVLDLCSKQNIPEIYLHVQTNN 120

Query: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 165
           DDAI FY KFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK
Sbjct: 121 DDAIKFYTKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 164

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BLAST of MS001286 vs. ExPASy Swiss-Prot
Match: Q0IIJ0 (N-alpha-acetyltransferase 50 OS=Bos taurus OX=9913 GN=NAA50 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.5e-41
Identity = 82/148 (55.41%), Postives = 105/148 (70.95%), Query Frame = 0


Query: 9   ISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVGSIACRLEK 68
           I L  V   N+ QLK+LN  +FPV YN+K+Y DVL  GE  KLAY++DI VG++ CR++ 
Sbjct: 6   IELGDVTPHNIKQLKRLNQVIFPVSYNDKFYKDVLEVGELAKLAYFNDIAVGAVCCRVDH 65

Query: 69  KENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQ-NITEIYLHVQTNNDDAINFY 128
            +N   R+YIMTLG LAPYR LGIG ++LNH L++C K      IYLHVQ +N+ AI+FY
Sbjct: 66  SQN-QKRLYIMTLGCLAPYRRLGIGTKMLNHVLNICEKDGTFDNIYLHVQISNESAIDFY 125

Query: 129 KKFGFEITETIQNYYANITPPDCYVLTK 156
           +KFGFEI ET +NYY  I P D +VL K
Sbjct: 126 RKFGFEIIETKKNYYKRIEPADAHVLQK 152

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BLAST of MS001286 vs. ExPASy Swiss-Prot
Match: Q9GZZ1 (N-alpha-acetyltransferase 50 OS=Homo sapiens OX=9606 GN=NAA50 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.5e-41
Identity = 82/148 (55.41%), Postives = 105/148 (70.95%), Query Frame = 0


Query: 9   ISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVGSIACRLEK 68
           I L  V   N+ QLK+LN  +FPV YN+K+Y DVL  GE  KLAY++DI VG++ CR++ 
Sbjct: 6   IELGDVTPHNIKQLKRLNQVIFPVSYNDKFYKDVLEVGELAKLAYFNDIAVGAVCCRVDH 65

Query: 69  KENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQ-NITEIYLHVQTNNDDAINFY 128
            +N   R+YIMTLG LAPYR LGIG ++LNH L++C K      IYLHVQ +N+ AI+FY
Sbjct: 66  SQN-QKRLYIMTLGCLAPYRRLGIGTKMLNHVLNICEKDGTFDNIYLHVQISNESAIDFY 125

Query: 129 KKFGFEITETIQNYYANITPPDCYVLTK 156
           +KFGFEI ET +NYY  I P D +VL K
Sbjct: 126 RKFGFEIIETKKNYYKRIEPADAHVLQK 152

[Close]

BLAST of MS001286 vs. ExPASy Swiss-Prot
Match: Q6PGB6 (N-alpha-acetyltransferase 50 OS=Mus musculus OX=10090 GN=Naa50 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.5e-41
Identity = 82/148 (55.41%), Postives = 105/148 (70.95%), Query Frame = 0


Query: 9   ISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVGSIACRLEK 68
           I L  V   N+ QLK+LN  +FPV YN+K+Y DVL  GE  KLAY++DI VG++ CR++ 
Sbjct: 6   IELGDVTPHNIKQLKRLNQVIFPVSYNDKFYKDVLEVGELAKLAYFNDIAVGAVCCRVDH 65

Query: 69  KENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQ-NITEIYLHVQTNNDDAINFY 128
            +N   R+YIMTLG LAPYR LGIG ++LNH L++C K      IYLHVQ +N+ AI+FY
Sbjct: 66  SQN-QKRLYIMTLGCLAPYRRLGIGTKMLNHVLNICEKDGTFDNIYLHVQISNESAIDFY 125

Query: 129 KKFGFEITETIQNYYANITPPDCYVLTK 156
           +KFGFEI ET +NYY  I P D +VL K
Sbjct: 126 RKFGFEIIETKKNYYKRIEPADAHVLQK 152

[Close]

BLAST of MS001286 vs. ExPASy Swiss-Prot
Match: Q5RF28 (N-alpha-acetyltransferase 50 OS=Pongo abelii OX=9601 GN=NAA50 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.5e-41
Identity = 82/148 (55.41%), Postives = 105/148 (70.95%), Query Frame = 0


Query: 9   ISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVGSIACRLEK 68
           I L  V   N+ QLK+LN  +FPV YN+K+Y DVL  GE  KLAY++DI VG++ CR++ 
Sbjct: 6   IELGDVTPHNIKQLKRLNQVIFPVSYNDKFYKDVLEVGELAKLAYFNDIAVGAVCCRVDH 65

Query: 69  KENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQ-NITEIYLHVQTNNDDAINFY 128
            +N   R+YIMTLG LAPYR LGIG ++LNH L++C K      IYLHVQ +N+ AI+FY
Sbjct: 66  SQN-QKRLYIMTLGCLAPYRRLGIGTKMLNHVLNICEKDGTFDNIYLHVQISNESAIDFY 125

Query: 129 KKFGFEITETIQNYYANITPPDCYVLTK 156
           +KFGFEI ET +NYY  I P D +VL K
Sbjct: 126 RKFGFEIIETKKNYYKRIEPADAHVLQK 152

[Close]

BLAST of MS001286 vs. ExPASy Swiss-Prot
Match: Q6GP53 (N-alpha-acetyltransferase 50 OS=Xenopus laevis OX=8355 GN=naa50 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.5e-41
Identity = 82/148 (55.41%), Postives = 105/148 (70.95%), Query Frame = 0


Query: 9   ISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVGSIACRLEK 68
           I L  V   N+ QLK+LN  +FPV YN+K+Y DVL  GE  KLAY++DI VG++ CR++ 
Sbjct: 6   IELGDVTPHNIKQLKRLNQVIFPVSYNDKFYKDVLEVGELAKLAYFNDIAVGAVCCRVDH 65

Query: 69  KENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQ-NITEIYLHVQTNNDDAINFY 128
            +N   R+YIMTLG LAPYR LGIG ++LNH L++C K      IYLHVQ +N+ AI+FY
Sbjct: 66  SQN-QKRLYIMTLGCLAPYRRLGIGTKMLNHVLNICEKDGTFDNIYLHVQISNESAIDFY 125

Query: 129 KKFGFEITETIQNYYANITPPDCYVLTK 156
           +KFGFEI ET +NYY  I P D +VL K
Sbjct: 126 RKFGFEIIETKKNYYKRIEPADAHVLQK 152

[Close]

BLAST of MS001286 vs. ExPASy TrEMBL
Match: A0A6J1BVY2 (N-alpha-acetyltransferase 50 OS=Momordica charantia OX=3673 GN=LOC111005202 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 8.1e-88
Identity = 163/164 (99.39%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG 60
           MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG
Sbjct: 1   MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG 60

Query: 61  SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQNITEIYLHVQTNN 120
           SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIG+RLLNHALDLCSKQNITEIYLHVQTNN
Sbjct: 61  SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGSRLLNHALDLCSKQNITEIYLHVQTNN 120

Query: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 165
           DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK
Sbjct: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 164

[Close]

BLAST of MS001286 vs. ExPASy TrEMBL
Match: A0A5A7VCP3 (N-alpha-acetyltransferase 50 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002810 PE=4 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 6.4e-85
Identity = 157/164 (95.73%), Postives = 160/164 (97.56%), Query Frame = 0


Query: 1   MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG 60
           MG GR+VPISLDGVRDKNLMQLKKLNTALFPVRYNEKYY DVLASGEFTKLAYYSDICVG
Sbjct: 1   MGVGRQVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYADVLASGEFTKLAYYSDICVG 60

Query: 61  SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQNITEIYLHVQTNN 120
           SIACRLEKKE+GSVRVYIMTLGVLAPYRGLGIG+RLLNH LDLCSKQNI EIYLHVQTNN
Sbjct: 61  SIACRLEKKEHGSVRVYIMTLGVLAPYRGLGIGSRLLNHVLDLCSKQNIAEIYLHVQTNN 120

Query: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 165
           DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK
Sbjct: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 164

[Close]

BLAST of MS001286 vs. ExPASy TrEMBL
Match: A0A0A0LSD7 (N-acetyltransferase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379910 PE=4 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 6.4e-85
Identity = 157/164 (95.73%), Postives = 160/164 (97.56%), Query Frame = 0


Query: 1   MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG 60
           MG GR+VPISLDGVRDKNLMQLKKLNTALFPVRYNEKYY DVLASGEFTKLAYYSDICVG
Sbjct: 1   MGVGRQVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYADVLASGEFTKLAYYSDICVG 60

Query: 61  SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQNITEIYLHVQTNN 120
           SIACRLEKKE+GSVRVYIMTLGVLAPYRGLGIG+RLLNH LDLCSKQNI EIYLHVQTNN
Sbjct: 61  SIACRLEKKEHGSVRVYIMTLGVLAPYRGLGIGSRLLNHVLDLCSKQNIAEIYLHVQTNN 120

Query: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 165
           DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK
Sbjct: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 164

[Close]

BLAST of MS001286 vs. ExPASy TrEMBL
Match: A0A1S3BBJ0 (N-alpha-acetyltransferase 50 OS=Cucumis melo OX=3656 GN=LOC103488274 PE=4 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 6.4e-85
Identity = 157/164 (95.73%), Postives = 160/164 (97.56%), Query Frame = 0


Query: 1   MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG 60
           MG GR+VPISLDGVRDKNLMQLKKLNTALFPVRYNEKYY DVLASGEFTKLAYYSDICVG
Sbjct: 1   MGVGRQVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYADVLASGEFTKLAYYSDICVG 60

Query: 61  SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQNITEIYLHVQTNN 120
           SIACRLEKKE+GSVRVYIMTLGVLAPYRGLGIG+RLLNH LDLCSKQNI EIYLHVQTNN
Sbjct: 61  SIACRLEKKEHGSVRVYIMTLGVLAPYRGLGIGSRLLNHVLDLCSKQNIAEIYLHVQTNN 120

Query: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 165
           DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK
Sbjct: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 164

[Close]

BLAST of MS001286 vs. ExPASy TrEMBL
Match: A0A6J1K2M5 (N-alpha-acetyltransferase 50-like OS=Cucurbita maxima OX=3661 GN=LOC111491844 PE=4 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.3e-81
Identity = 152/164 (92.68%), Postives = 157/164 (95.73%), Query Frame = 0


Query: 1   MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG 60
           M AGREVPISLD VRDKNLMQLKKLNTALFPVRYNEKYY DVLASG+FTKLAYYSDICVG
Sbjct: 1   MVAGREVPISLDEVRDKNLMQLKKLNTALFPVRYNEKYYADVLASGDFTKLAYYSDICVG 60

Query: 61  SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQNITEIYLHVQTNN 120
           SIACRLEKK++GSVRVYIMTLGVLAPYRGLGIG++LLNH LDLCSKQNI EIYLHVQTNN
Sbjct: 61  SIACRLEKKDHGSVRVYIMTLGVLAPYRGLGIGSKLLNHVLDLCSKQNIPEIYLHVQTNN 120

Query: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 165
           DDAI FY KFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK
Sbjct: 121 DDAIKFYTKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 164

[Close]

BLAST of MS001286 vs. TAIR 10
Match: AT5G11340.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 275.8 bits (704), Expect = 2.3e-74
Identity = 128/164 (78.05%), Postives = 147/164 (89.63%), Query Frame = 0


Query: 1   MGAGREVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAYYSDICVG 60
           MGAGREV +SLDGVRDKNLMQLK LNT LFPVRYN+KYY D +A+GEFTKLAYY+DICVG
Sbjct: 1   MGAGREVSVSLDGVRDKNLMQLKILNTVLFPVRYNDKYYADAIAAGEFTKLAYYNDICVG 60

Query: 61  SIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQNITEIYLHVQTNN 120
           +IACRLEKKE+G++RVYIMTLGVLAPYRG+GIG+ LLNH LD+CSKQN+ EIYLHVQTNN
Sbjct: 61  AIACRLEKKESGAMRVYIMTLGVLAPYRGIGIGSNLLNHVLDMCSKQNMCEIYLHVQTNN 120

Query: 121 DDAINFYKKFGFEITETIQNYYANITPPDCYVLTKLITQTQTKK 165
           +DAI FYKKFGFEIT+TIQNYY NI P DCYV++K   Q++  K
Sbjct: 121 EDAIKFYKKFGFEITDTIQNYYINIEPRDCYVVSKSFAQSEANK 164

[Close]

BLAST of MS001286 vs. TAIR 10
Match: AT5G16800.2 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 2.3e-10
Identity = 39/161 (24.22%), Postives = 71/161 (44.10%), Query Frame = 0


Query: 5   REVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAY--------YSD 64
           R   I    +   +L +L++++  +FP+RY  +++ +V+  G+    A         +S+
Sbjct: 21  RRPTICFRPINPSDLERLEQIHRDIFPIRYESEFFQNVVNGGDIVSWAAVDRSRPDGHSE 80

Query: 65  ICVGSIAC--------------RLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALD 124
             +G +                R +  +     VYI+TLGV+  YR  GI   L+N  + 
Sbjct: 81  ELIGFVTAKIVLAKESEISDLIRYDSSKGEGTLVYILTLGVVETYRKRGIAKALINEVVK 140

Query: 125 LCSKQNITE-IYLHVQTNNDDAINFYKKFGFEITETIQNYY 143
             S   +   +YLHV  +N+ AI  YK+  F     +  +Y
Sbjct: 141 YSSGIPVCRGVYLHVIAHNNPAIRLYKRMSFRCVRRLHGFY 181

[Close]

BLAST of MS001286 vs. TAIR 10
Match: AT5G16800.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 2.3e-10
Identity = 39/161 (24.22%), Postives = 71/161 (44.10%), Query Frame = 0


Query: 5   REVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAY--------YSD 64
           R   I    +   +L +L++++  +FP+RY  +++ +V+  G+    A         +S+
Sbjct: 21  RRPTICFRPINPSDLERLEQIHRDIFPIRYESEFFQNVVNGGDIVSWAAVDRSRPDGHSE 80

Query: 65  ICVGSIAC--------------RLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALD 124
             +G +                R +  +     VYI+TLGV+  YR  GI   L+N  + 
Sbjct: 81  ELIGFVTAKIVLAKESEISDLIRYDSSKGEGTLVYILTLGVVETYRKRGIAKALINEVVK 140

Query: 125 LCSKQNITE-IYLHVQTNNDDAINFYKKFGFEITETIQNYY 143
             S   +   +YLHV  +N+ AI  YK+  F     +  +Y
Sbjct: 141 YSSGIPVCRGVYLHVIAHNNPAIRLYKRMSFRCVRRLHGFY 181

[Close]

BLAST of MS001286 vs. TAIR 10
Match: AT5G16800.3 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 2.3e-10
Identity = 39/161 (24.22%), Postives = 71/161 (44.10%), Query Frame = 0


Query: 5   REVPISLDGVRDKNLMQLKKLNTALFPVRYNEKYYVDVLASGEFTKLAY--------YSD 64
           R   I    +   +L +L++++  +FP+RY  +++ +V+  G+    A         +S+
Sbjct: 21  RRPTICFRPINPSDLERLEQIHRDIFPIRYESEFFQNVVNGGDIVSWAAVDRSRPDGHSE 80

Query: 65  ICVGSIAC--------------RLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALD 124
             +G +                R +  +     VYI+TLGV+  YR  GI   L+N  + 
Sbjct: 81  ELIGFVTAKIVLAKESEISDLIRYDSSKGEGTLVYILTLGVVETYRKRGIAKALINEVVK 140

Query: 125 LCSKQNITE-IYLHVQTNNDDAINFYKKFGFEITETIQNYY 143
             S   +   +YLHV  +N+ AI  YK+  F     +  +Y
Sbjct: 141 YSSGIPVCRGVYLHVIAHNNPAIRLYKRMSFRCVRRLHGFY 181

[Close]

BLAST of MS001286 vs. TAIR 10
Match: AT2G38130.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 59.7 bits (143), Expect = 2.6e-09
Identity = 30/94 (31.91%), Postives = 49/94 (52.13%), Query Frame = 0


Query: 51  LAYYSDICVGSIACRLEKKENGSVRVYIMTLGVLAPYRGLGIGNRLLNHALDLCSKQNIT 110
           LA++   CVG+I C++      + R YI  L V+ PYRG GI + L+  A+    +    
Sbjct: 60  LAFHKGKCVGTIVCKMGDHRQ-TFRGYIAMLVVIKPYRGRGIASELVTRAIKAMMESGCE 119

Query: 111 EIYLHVQTNNDDAINFYKKFGFEITETIQNYYAN 145
           E+ L  + +N  A+  Y + GF   + + +YY N
Sbjct: 120 EVTLEAEVSNKGALALYGRLGFIRAKRLYHYYLN 152

The following BLAST results are available for this feature:

BLAST of MS001286 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132323.1	1.7e-87	99.39	N-alpha-acetyltransferase 50 [Momordica charantia]	[more]
XP_038884070.1	4.6e-85	96.34	N-alpha-acetyltransferase 50 [Benincasa hispida]	[more]
XP_004138740.1	1.3e-84	95.73	N-alpha-acetyltransferase 50 [Cucumis sativus] >XP_008445150.1 PREDICTED: N-alph...	[more]
XP_023545587.1	3.1e-81	93.29	N-alpha-acetyltransferase 50-like [Cucurbita pepo subsp. pepo] >KAG6598593.1 N-a...	[more]
XP_022996667.1	6.8e-81	92.68	N-alpha-acetyltransferase 50-like [Cucurbita maxima]	[more]

BLAST of MS001286 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0IIJ0	2.5e-41	55.41	N-alpha-acetyltransferase 50 OS=Bos taurus OX=9913 GN=NAA50 PE=2 SV=1	[more]
Q9GZZ1	2.5e-41	55.41	N-alpha-acetyltransferase 50 OS=Homo sapiens OX=9606 GN=NAA50 PE=1 SV=1	[more]
Q6PGB6	2.5e-41	55.41	N-alpha-acetyltransferase 50 OS=Mus musculus OX=10090 GN=Naa50 PE=1 SV=1	[more]
Q5RF28	2.5e-41	55.41	N-alpha-acetyltransferase 50 OS=Pongo abelii OX=9601 GN=NAA50 PE=2 SV=1	[more]
Q6GP53	2.5e-41	55.41	N-alpha-acetyltransferase 50 OS=Xenopus laevis OX=8355 GN=naa50 PE=2 SV=1	[more]

BLAST of MS001286 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVY2	8.1e-88	99.39	N-alpha-acetyltransferase 50 OS=Momordica charantia OX=3673 GN=LOC111005202 PE=4...	[more]
A0A5A7VCP3	6.4e-85	95.73	N-alpha-acetyltransferase 50 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]
A0A0A0LSD7	6.4e-85	95.73	N-acetyltransferase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_...	[more]
A0A1S3BBJ0	6.4e-85	95.73	N-alpha-acetyltransferase 50 OS=Cucumis melo OX=3656 GN=LOC103488274 PE=4 SV=1	[more]
A0A6J1K2M5	3.3e-81	92.68	N-alpha-acetyltransferase 50-like OS=Cucurbita maxima OX=3661 GN=LOC111491844 PE...	[more]

BLAST of MS001286 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G11340.1	2.3e-74	78.05	Acyl-CoA N-acyltransferases (NAT) superfamily protein	[more]
AT5G16800.2	2.3e-10	24.22	Acyl-CoA N-acyltransferases (NAT) superfamily protein	[more]
AT5G16800.1	2.3e-10	24.22	Acyl-CoA N-acyltransferases (NAT) superfamily protein	[more]
AT5G16800.3	2.3e-10	24.22	Acyl-CoA N-acyltransferases (NAT) superfamily protein	[more]
AT2G38130.1	2.6e-09	31.91	Acyl-CoA N-acyltransferases (NAT) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000182	GNAT domain	PFAM	PF00583	Acetyltransf_1	coord: 51..132 e-value: 3.7E-16 score: 59.4
IPR000182	GNAT domain	PROSITE	PS51186	GNAT	coord: 9..158 score: 21.884481
None	No IPR available	GENE3D	3.40.630.30		coord: 5..158 e-value: 4.4E-53 score: 181.6
None	No IPR available	PANTHER	PTHR42919:SF22	SUMO-CONJUGATING ENZYME SCE1	coord: 1..162
None	No IPR available	PANTHER	PTHR42919	N-ALPHA-ACETYLTRANSFERASE	coord: 1..162
None	No IPR available	CDD	cd04301	NAT_SF	coord: 51..115 e-value: 5.35702E-11 score: 53.4336
IPR016181	Acyl-CoA N-acyltransferase	SUPERFAMILY	55729	Acyl-CoA N-acyltransferases (Nat)	coord: 17..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001286.1	MS001286.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008080	N-acetyltransferase activity