MS001250 (gene) Bitter gourd (TR) v1

Overview


Name	MS001250
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Aminoacyl-tRNA hydrolase
Location	scaffold36: 2662705 .. 2663985 (+)
RNA-Seq Expression	MS001250
Synteny	MS001250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACATCTGTAGTTTCAGCATTCTCATTCTCCATCCCCAATTTCTGCAGGCTCTGTTCAAGGATTAGGGCTTCGCCATCTTCTTCACTCCCTCTTCGCTATCGGAACGGAAATTCCAGGTTATTTAGCTCGGTTTCGATGAGTGAATCCGTTGAAAGTAATTCCGTTCCTCCAACGGACGACGGCGGAGCGAAGGCGGAGGAACGAGCCAAGGAGGAGACCGCCGATGTTTTGGTTCAGTATGTGGTGCTCCGGAGAGATCTGATCGACTCGTGGCCGCTCGGAAGCGTCGTAACTCAAGGATGCCATGCTTCAGTCTCCGCCATTTGGTTGAACAAGGACGATTCCCACACTTCTGAGTACTGTAGTCCTGGTAACATCGATTCTATGCACAAGGTAAGTCAAGCTTTTAACTCCATTTTTGATTTTGATTTTCAAGTAAAGCACCTCGAACAATGTAGTATGCGTTCGTGGCTACTATCTTTGTATGTTGATGTGCCGAATATTTGGTTTCTCGGTGCTCTATTTAGTCGGTCTGACAGTTGAGATATTCTGAGCAATTTGGAAGTTTTATGCTGTGGTCGCTGGTTGATTTATTCAGTGCTGGGAAGTACATTTTATTTGTAGTTTTCGATCTTTCTTCCGTCAGGTGTTTGAACATTTCGGAAGAACAATGGTTGATTGGAAAGCCTATTCTTATTTCTGTTCGCGTTGTTTTCCATGTGAAGAATTATTAAACTGTGTTCTGATTTTCCACCTTAATTCCACAATTGTATCCTTCCAGAATCTACATTATAATCGGAGGTCAAGGAGAGCTCATTTATCTCATATTTCTGCTTGTACATTTTCTTTTAGTTTTATGTGGTCTTCAATCATTTTCATGGTTAAAATGCATTCCTCTTCATTCGCATTTTTGTTAAACGATTGAACTGCCAAACCTCTTGTTTATTGGCAAATTCCTTGCATATCAGATTAATATCTTGTGCAACTTTGTTGAATTAACTGGCATACTTCCGTTGATCTATTAAGCCATACTGGACTTTTTGATGCCTGAAGGTGGGTTTTAAGAAGCTGATTGTATTTCACTTTTTCCATATGTAGGTTACTGTCGAGGTGAAAGGAGAAACCCAGATCGTTAACTTGTCAGAGAAACTCAAAGCAAATTCAATCGCCCATAAACTGTGGATCGAACAACCCGAGAACATTCCAACATGCCTTGCCACAAAGCCCTACCCCAAATCTCTAGTATCAGCATTTTTTAAAAAGCTGAAACTCTGTAAG

mRNA sequence

ATGACATCTGTAGTTTCAGCATTCTCATTCTCCATCCCCAATTTCTGCAGGCTCTGTTCAAGGATTAGGGCTTCGCCATCTTCTTCACTCCCTCTTCGCTATCGGAACGGAAATTCCAGGTTATTTAGCTCGGTTTCGATGAGTGAATCCGTTGAAAGTAATTCCGTTCCTCCAACGGACGACGGCGGAGCGAAGGCGGAGGAACGAGCCAAGGAGGAGACCGCCGATGTTTTGGTTCAGTATGTGGTGCTCCGGAGAGATCTGATCGACTCGTGGCCGCTCGGAAGCGTCGTAACTCAAGGATGCCATGCTTCAGTCTCCGCCATTTGGTTGAACAAGGACGATTCCCACACTTCTGAGTACTGTAGTCCTGGTAACATCGATTCTATGCACAAGGTTACTGTCGAGGTGAAAGGAGAAACCCAGATCGTTAACTTGTCAGAGAAACTCAAAGCAAATTCAATCGCCCATAAACTGTGGATCGAACAACCCGAGAACATTCCAACATGCCTTGCCACAAAGCCCTACCCCAAATCTCTAGTATCAGCATTTTTTAAAAAGCTGAAACTCTGTAAG

Coding sequence (CDS)

ATGACATCTGTAGTTTCAGCATTCTCATTCTCCATCCCCAATTTCTGCAGGCTCTGTTCAAGGATTAGGGCTTCGCCATCTTCTTCACTCCCTCTTCGCTATCGGAACGGAAATTCCAGGTTATTTAGCTCGGTTTCGATGAGTGAATCCGTTGAAAGTAATTCCGTTCCTCCAACGGACGACGGCGGAGCGAAGGCGGAGGAACGAGCCAAGGAGGAGACCGCCGATGTTTTGGTTCAGTATGTGGTGCTCCGGAGAGATCTGATCGACTCGTGGCCGCTCGGAAGCGTCGTAACTCAAGGATGCCATGCTTCAGTCTCCGCCATTTGGTTGAACAAGGACGATTCCCACACTTCTGAGTACTGTAGTCCTGGTAACATCGATTCTATGCACAAGGTTACTGTCGAGGTGAAAGGAGAAACCCAGATCGTTAACTTGTCAGAGAAACTCAAAGCAAATTCAATCGCCCATAAACTGTGGATCGAACAACCCGAGAACATTCCAACATGCCTTGCCACAAAGCCCTACCCCAAATCTCTAGTATCAGCATTTTTTAAAAAGCTGAAACTCTGTAAG

Protein sequence

MTSVVSAFSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKVTVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK

Homology

[Close]

BLAST of MS001250 vs. NCBI nr
Match: XP_022132277.1 (uncharacterized protein LOC111005172 [Momordica charantia])

HSP 1 Score: 379.8 bits (974), Expect = 1.4e-101
Identity = 191/192 (99.48%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MTSVVSAFSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTD 60
           MTSVVSAFSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTD
Sbjct: 40  MTSVVSAFSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTD 99

Query: 61  DGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSE 120
           DGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSE
Sbjct: 100 DGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSE 159

Query: 121 YCSPGNIDSMHKVTVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSL 180
           YCSPGNIDSMHK+TVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSL
Sbjct: 160 YCSPGNIDSMHKITVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSL 219

Query: 181 VSAFFKKLKLCK 193
           VSAFFKKLKLCK
Sbjct: 220 VSAFFKKLKLCK 231

[Close]

BLAST of MS001250 vs. NCBI nr
Match: XP_038884124.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Benincasa hispida])

HSP 1 Score: 293.5 bits (750), Expect = 1.3e-75
Identity = 157/196 (80.10%), Postives = 165/196 (84.18%), Query Frame = 0


Query: 1   MTSVVSA--FSFSIPNFCRL--CSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSV 60
           MT V+SA  F+FSI    RL  CS  R SPSSSL LR  N NS  FSS SMS+S E+NSV
Sbjct: 1   MTCVISASRFAFSISKSTRLCSCSSPRVSPSSSLSLRSWNRNSNPFSSASMSQSAETNSV 60

Query: 61  PPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDS 120
           PP DDGGAKA     EE ADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDD 
Sbjct: 61  PPPDDGGAKA-----EEIADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDP 120

Query: 121 HTSEYCSPGNIDSMHKVTVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPY 180
           HTS+YCSP NIDSMHKVT+EVKGETQIVNLSEKLKANSI HKLWIEQPENIPTCLATKPY
Sbjct: 121 HTSDYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWIEQPENIPTCLATKPY 180

Query: 181 PKSLVSAFFKKLKLCK 193
           PKS+VS FFKKLKLCK
Sbjct: 181 PKSIVSPFFKKLKLCK 191

[Close]

BLAST of MS001250 vs. NCBI nr
Match: XP_023546189.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 292.4 bits (747), Expect = 2.9e-75
Identity = 156/196 (79.59%), Postives = 165/196 (84.18%), Query Frame = 0


Query: 1   MTSVVSA--FSFSIPNFCRL--CSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSV 60
           M SV+SA  F+FSI    +L  CSR R  PSSSL LR RN NS LFSS SMSES ESNS 
Sbjct: 12  MISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAESNSA 71

Query: 61  PPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDS 120
           PP DDG         E+TADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSA+WLNKDD 
Sbjct: 72  PPPDDG--------VEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAVWLNKDDP 131

Query: 121 HTSEYCSPGNIDSMHKVTVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPY 180
           HTSEYCSP NIDSMHKVT+EVKGETQIVNLSEKLKA+SIAHKLWIEQPENIPTCLATKPY
Sbjct: 132 HTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPENIPTCLATKPY 191

Query: 181 PKSLVSAFFKKLKLCK 193
           PKS+VSAFFKKLKLCK
Sbjct: 192 PKSVVSAFFKKLKLCK 199

[Close]

BLAST of MS001250 vs. NCBI nr
Match: XP_022962062.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita moschata])

HSP 1 Score: 287.7 bits (735), Expect = 7.2e-74
Identity = 154/196 (78.57%), Postives = 164/196 (83.67%), Query Frame = 0


Query: 1   MTSVVSA--FSFSIPNFCRL--CSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSV 60
           M SV+SA  F+FSI    +L  CSR R  PSSSL LR RN NS LFSS SMSES ESNS 
Sbjct: 1   MISVISASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSDSMSESAESNSA 60

Query: 61  PPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDS 120
           PP DD        + E+TADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSA+WLNKDD 
Sbjct: 61  PPPDD--------SVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAVWLNKDDP 120

Query: 121 HTSEYCSPGNIDSMHKVTVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPY 180
           HT EYCSP NIDSMHKVT+EVKGETQIVNLSEKL+ANSIAHKLWIEQPENIPTCLATKPY
Sbjct: 121 HTFEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLRANSIAHKLWIEQPENIPTCLATKPY 180

Query: 181 PKSLVSAFFKKLKLCK 193
           PKS+VSAFFKKLKLCK
Sbjct: 181 PKSVVSAFFKKLKLCK 188

[Close]

BLAST of MS001250 vs. NCBI nr
Match: XP_023002512.1 (putative peptidyl-tRNA hydrolase PTRHD1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 287.0 bits (733), Expect = 1.2e-73
Identity = 154/194 (79.38%), Postives = 161/194 (82.99%), Query Frame = 0


Query: 1   MTSVVSA--FSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPP 60
           M SV+SA  F+FSI N  RLCS  R    SS+ L   N NS  FSS SMSES ESNSV P
Sbjct: 1   MASVISASRFTFSISNSARLCSNARVWLPSSICLLSWNWNSISFSSASMSESAESNSVLP 60

Query: 61  TDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHT 120
            DDGGAKA     EE ADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDD HT
Sbjct: 61  LDDGGAKA-----EEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHT 120

Query: 121 SEYCSPGNIDSMHKVTVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPK 180
           SEYCSP NIDSMHKVT+EVKGETQIVNLSEKLKANSI HKLW+EQPENIPTCLA+KPYPK
Sbjct: 121 SEYCSPQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPK 180

Query: 181 SLVSAFFKKLKLCK 193
           S+VS FFKKLKLCK
Sbjct: 181 SVVSPFFKKLKLCK 189

[Close]

BLAST of MS001250 vs. ExPASy Swiss-Prot
Match: Q3SZ85 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Bos taurus OX=9913 GN=PTRHD1 PE=2 SV=2)

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-26
Identity = 59/119 (49.58%), Postives = 81/119 (68.07%), Query Frame = 0


Query: 77  VLVQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKV 136
           +LVQY+VLR+DL     SWP G+VV Q CHA+ +A+ L++D  HT+ Y     ++ M KV
Sbjct: 24  ILVQYLVLRKDLTQPPFSWPAGAVVAQACHAATAALHLHRDHPHTAAYLR--ELERMRKV 83

Query: 137 TVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK 193
            +E   ET +  L+E L+  +I HKLW+EQPENIPTC+A +PYPK  VS + KK +L K
Sbjct: 84  VLEAPDETTLKLLAETLQQKNIDHKLWMEQPENIPTCIALRPYPKEEVSQYLKKFRLFK 140

[Close]

BLAST of MS001250 vs. ExPASy Swiss-Prot
Match: D3Z4S3 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Mus musculus OX=10090 GN=Ptrhd1 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.4e-24
Identity = 56/119 (47.06%), Postives = 78/119 (65.55%), Query Frame = 0


Query: 77  VLVQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKV 136
           +LVQY+VLR+DL     SWP G++V Q CHA+ +A+ L++D  HT+ Y     +  M KV
Sbjct: 24  ILVQYLVLRKDLSQAPFSWPTGALVAQACHAATAALHLHRDHPHTAAYLR--ELGRMRKV 83

Query: 137 TVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK 193
            +E   ET +  L+E L+  +I H LW+EQPENI TC+A +PYPK  VS + KK +L K
Sbjct: 84  VLEAADETTLKELAETLQQKNIDHTLWLEQPENIATCIALRPYPKEEVSQYLKKFRLFK 140

[Close]

BLAST of MS001250 vs. ExPASy Swiss-Prot
Match: Q6GMV3 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Homo sapiens OX=9606 GN=PTRHD1 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 9.3e-24
Identity = 55/119 (46.22%), Postives = 76/119 (63.87%), Query Frame = 0


Query: 77  VLVQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDSHTSEYCSPGNIDSMHKV 136
           VLVQY+VLR+DL     SWP G++V Q CHA+ +A+  ++D  HT+ Y     +  M KV
Sbjct: 24  VLVQYLVLRKDLSQAPFSWPAGALVAQACHAATAALHTHRDHPHTAAYLQ--ELGRMRKV 83

Query: 137 TVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSLVSAFFKKLKLCK 193
            +E   ET +  L+E L+  +I H LW+EQPENI TC+A +PYPK  V  + KK +L K
Sbjct: 84  VLEAPDETTLKELAETLQQKNIDHMLWLEQPENIATCIALRPYPKEEVGQYLKKFRLFK 140

[Close]

BLAST of MS001250 vs. ExPASy TrEMBL
Match: A0A6J1BTE6 (Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111005172 PE=4 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 6.8e-102
Identity = 191/192 (99.48%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MTSVVSAFSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTD 60
           MTSVVSAFSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTD
Sbjct: 40  MTSVVSAFSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPPTD 99

Query: 61  DGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSE 120
           DGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSE
Sbjct: 100 DGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHTSE 159

Query: 121 YCSPGNIDSMHKVTVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSL 180
           YCSPGNIDSMHK+TVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSL
Sbjct: 160 YCSPGNIDSMHKITVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPKSL 219

Query: 181 VSAFFKKLKLCK 193
           VSAFFKKLKLCK
Sbjct: 220 VSAFFKKLKLCK 231

[Close]

BLAST of MS001250 vs. ExPASy TrEMBL
Match: A0A6J1HBR1 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111462634 PE=4 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 3.5e-74
Identity = 154/196 (78.57%), Postives = 164/196 (83.67%), Query Frame = 0


Query: 1   MTSVVSA--FSFSIPNFCRL--CSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSV 60
           M SV+SA  F+FSI    +L  CSR R  PSSSL LR RN NS LFSS SMSES ESNS 
Sbjct: 1   MISVISASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSDSMSESAESNSA 60

Query: 61  PPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDS 120
           PP DD        + E+TADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSA+WLNKDD 
Sbjct: 61  PPPDD--------SVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAVWLNKDDP 120

Query: 121 HTSEYCSPGNIDSMHKVTVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPY 180
           HT EYCSP NIDSMHKVT+EVKGETQIVNLSEKL+ANSIAHKLWIEQPENIPTCLATKPY
Sbjct: 121 HTFEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLRANSIAHKLWIEQPENIPTCLATKPY 180

Query: 181 PKSLVSAFFKKLKLCK 193
           PKS+VSAFFKKLKLCK
Sbjct: 181 PKSVVSAFFKKLKLCK 188

[Close]

BLAST of MS001250 vs. ExPASy TrEMBL
Match: A0A6J1KJQ6 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 6.0e-74
Identity = 154/194 (79.38%), Postives = 161/194 (82.99%), Query Frame = 0


Query: 1   MTSVVSA--FSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPP 60
           M SV+SA  F+FSI N  RLCS  R    SS+ L   N NS  FSS SMSES ESNSV P
Sbjct: 1   MASVISASRFTFSISNSARLCSNARVWLPSSICLLSWNWNSISFSSASMSESAESNSVLP 60

Query: 61  TDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHT 120
            DDGGAKA     EE ADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDD HT
Sbjct: 61  LDDGGAKA-----EEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHT 120

Query: 121 SEYCSPGNIDSMHKVTVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPK 180
           SEYCSP NIDSMHKVT+EVKGETQIVNLSEKLKANSI HKLW+EQPENIPTCLA+KPYPK
Sbjct: 121 SEYCSPQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPK 180

Query: 181 SLVSAFFKKLKLCK 193
           S+VS FFKKLKLCK
Sbjct: 181 SVVSPFFKKLKLCK 189

[Close]

BLAST of MS001250 vs. ExPASy TrEMBL
Match: A0A6J1GJ11 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454312 PE=4 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.7e-73
Identity = 154/194 (79.38%), Postives = 161/194 (82.99%), Query Frame = 0


Query: 1   MTSVVSA--FSFSIPNFCRLCSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSVPP 60
           M SV+SA  F+FSI N  RLC   R    SS+ LR  N NS  FSS SMSES ESNSV P
Sbjct: 1   MASVISASRFTFSISNSLRLCWNARVWLPSSICLRSWNWNSISFSSASMSESGESNSVLP 60

Query: 61  TDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDSHT 120
            DDGGAKA     EE ADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDD HT
Sbjct: 61  LDDGGAKA-----EEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHT 120

Query: 121 SEYCSPGNIDSMHKVTVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPYPK 180
           SEYCSP NIDSMHKVT+EVKGETQIVNLSEKLKANSI HKLW+EQPENIPTCLA+KPYPK
Sbjct: 121 SEYCSPQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPK 180

Query: 181 SLVSAFFKKLKLCK 193
           S+VS FFKKLKLCK
Sbjct: 181 SVVSPFFKKLKLCK 189

[Close]

BLAST of MS001250 vs. ExPASy TrEMBL
Match: A0A6J1K304 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491924 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 2.3e-73
Identity = 154/196 (78.57%), Postives = 161/196 (82.14%), Query Frame = 0


Query: 1   MTSVVSA--FSFSIPNFCRL--CSRIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSV 60
           M SV+SA  F+FSI    +L  CSR R  PSSSL LR RN NS LFSS SMSES ESNS 
Sbjct: 1   MISVISASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSASMSESAESNSA 60

Query: 61  PPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDS 120
           PP DD          E+ ADVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDD 
Sbjct: 61  PPPDD--------IVEKAADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDP 120

Query: 121 HTSEYCSPGNIDSMHKVTVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPY 180
            TSEYCSP NIDSMHKVT+EVKGETQIVNLSEKLK NSIAHKLWIEQPENIPTCLATKPY
Sbjct: 121 QTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKENSIAHKLWIEQPENIPTCLATKPY 180

Query: 181 PKSLVSAFFKKLKLCK 193
           PKS+VSAFFKKLKLCK
Sbjct: 181 PKSVVSAFFKKLKLCK 188

[Close]

BLAST of MS001250 vs. TAIR 10
Match: AT5G10700.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 201.8 bits (512), Expect = 4.9e-52
Identity = 113/196 (57.65%), Postives = 137/196 (69.90%), Query Frame = 0


Query: 1   MTSVVSAFSFSIPNFCRLCS----RIRASPSSSLPLRYRNGNSRLFSSVSMSESVESNSV 60
           M + V + +F  P   R  S    RIR   S+      R  + R F++ S S S ++  +
Sbjct: 1   MATAVLSAAFRFPIVTRRLSAPAPRIRFYHST-----IRFAHFRRFTTASPSMSQQTGEI 60

Query: 61  PPTDDGGAKAEERAKEETADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDS 120
                  A  +  + E+  DV+VQYVVLRRDLIDSWPLGSVVTQGCHASV+AIW  KDD 
Sbjct: 61  ------DAVVDASSAEKPDDVVVQYVVLRRDLIDSWPLGSVVTQGCHASVAAIWSFKDDP 120

Query: 121 HTSEYCSPGNIDSMHKVTVEVKGETQIVNLSEKLKANSIAHKLWIEQPENIPTCLATKPY 180
            T +YC P +IDSMHKVT+EVKGETQ++NLSEKLK   I+HKLW+EQPENIPTC+ATKPY
Sbjct: 121 VTLQYCDPQHIDSMHKVTLEVKGETQMMNLSEKLKLGGISHKLWMEQPENIPTCIATKPY 180

Query: 181 PKSLVSAFFKKLKLCK 193
           PKS VS+FFKKLKLCK
Sbjct: 181 PKSQVSSFFKKLKLCK 185

The following BLAST results are available for this feature:

BLAST of MS001250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132277.1	1.4e-101	99.48	uncharacterized protein LOC111005172 [Momordica charantia]	[more]
XP_038884124.1	1.3e-75	80.10	putative peptidyl-tRNA hydrolase PTRHD1 [Benincasa hispida]	[more]
XP_023546189.1	2.9e-75	79.59	putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita pepo subsp. pepo]	[more]
XP_022962062.1	7.2e-74	78.57	putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita moschata]	[more]
XP_023002512.1	1.2e-73	79.38	putative peptidyl-tRNA hydrolase PTRHD1 isoform X1 [Cucurbita maxima]	[more]

BLAST of MS001250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3SZ85	1.5e-26	49.58	Putative peptidyl-tRNA hydrolase PTRHD1 OS=Bos taurus OX=9913 GN=PTRHD1 PE=2 SV=...	[more]
D3Z4S3	1.4e-24	47.06	Putative peptidyl-tRNA hydrolase PTRHD1 OS=Mus musculus OX=10090 GN=Ptrhd1 PE=1 ...	[more]
Q6GMV3	9.3e-24	46.22	Putative peptidyl-tRNA hydrolase PTRHD1 OS=Homo sapiens OX=9606 GN=PTRHD1 PE=1 S...	[more]

BLAST of MS001250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BTE6	6.8e-102	99.48	Aminoacyl-tRNA hydrolase OS=Momordica charantia OX=3673 GN=LOC111005172 PE=4 SV=...	[more]
A0A6J1HBR1	3.5e-74	78.57	Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111462634 PE=4 SV=1	[more]
A0A6J1KJQ6	6.0e-74	79.38	Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1	[more]
A0A6J1GJ11	1.7e-73	79.38	Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454312 PE=4 SV=1	[more]
A0A6J1K304	2.3e-73	78.57	Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491924 PE=4 SV=1	[more]

BLAST of MS001250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10700.1	4.9e-52	57.65	Peptidyl-tRNA hydrolase II (PTH2) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002833	Peptidyl-tRNA hydrolase, PTH2	PFAM	PF01981	PTH2	coord: 79..190 e-value: 8.2E-24 score: 84.2
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	GENE3D	3.40.1490.10	Bit1	coord: 78..191 e-value: 1.1E-17 score: 66.1
IPR023476	Peptidyl-tRNA hydrolase II domain superfamily	SUPERFAMILY	102462	Peptidyl-tRNA hydrolase II	coord: 78..191
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 49..70
None	No IPR available	CDD	cd02429	PTH2_like	coord: 78..191 e-value: 5.67832E-66 score: 196.471
IPR042237	Putative peptidyl-tRNA hydrolase PTRHD1	PANTHER	PTHR46194	PEPTIDYL-TRNA HYDROLASE PTRHD1-RELATED	coord: 47..192

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001250.1	MS001250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity