MS001133 (gene) Bitter gourd (TR) v1

Overview


Name	MS001133
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Rhamnogalacturonan endolyase
Location	scaffold36: 1694069 .. 1698015 (+)
RNA-Seq Expression	MS001133
Synteny	MS001133

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGCCTCTGGGTGTGCAACTATACGTCACAGAACGTCAGGTACGTACGTCCCTCCCTCTCCTTTTCTCTATATTTCTTCTCCCCTTTATGTCAAAACGGAGAAATGTGCAGGTGGTGATGGATAATGGCATAGTTCAAGTCACGTTTTCAAACCCAGATGGCATAGTGACCGGAATCCGATACAATGGAGTCGACAACTTGCTCGAAATTCTCAACGACGAATATAACAGAGGGTATACTGTAGTGAACATGTCGTCCCATTACAGTAATTCTTCCATGCATAAATACGCTGCTTCACAATAATATTATTGTGCATGCAGGTATTGGGACCTCGTGTGGAGCCCATCCGCCTCCACAACTGGATCGTTCGATGTGTATGTATTCTGTTCTCTTTTACAAGTAGGCTCTCTTACAGATTTCTGGCTCATAAGTTTTGAGTAGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTANNNNNNNNNTTTTTTTTTTTTTTTTTTTGTGCTTGCAGGATAAAGGCTACGAGTTTTGAGGTTATAGTGGAAAATGAAGACCAAGTGGAGCTCTCCTTTACACGAAAATGGGATCTGTCTCTCGAGGGAAAGCTTGTGCCTTTAGAGATTGATAAAAGGTTAGATTGATTCGAATCTTGCTTTGGCAAGAAAGTTAAATTCCAGACCTTTTTCTTATGAATGGATTTGTAGCTATGTGTAGGTTTGTAATGCTTCGGGGTTCGTCAGGGTTCTACTCTTATGCTATTTACCAACACTTGAAAGAATGGCCTGGTTTCAACCTTGGTGAAACCAGACTCGCCTTCAAACTCAGAAAAGACAAGTAACACCCAAATTCTCTACATTATTTCATTGAATGCTTGCTTTAGCTTAAATAGTACCTAATTTTTTTTGGATAATTCTTAATCTTATTCTTAATTTTTTTGGATAATTCTTATGTTTTTGTATATAATAATAAAAGAATAGTTTGACTTTTTTTTTTCTTTTGAGGGATAAATTTAATTGGGCATTTTTAATATATTAAACATAAAAATAAGCATTTTAAAAGATTTAAAGACAAAATTGAGAATTAAAAGGGGGACTACTTTGGTATATTTGACCTTTAATTTATAGCATTTGTTTGTAATTTGAAAATTTGAACAATGCATATGGATAATTGTCGTAGGTTCCACTATATGGCCATGGCAGACAACAGACAGAGATCCATGCCGCTGCCAGATGACCGGTCTCCGCCAAGAGGCCGAGCCCTGGCCTATCCGGAGGCTGTCCTGCTCGTCGACCCCGTCGAACCTGAGCTCCAGGGAGAGGTCCATTATTGAATCTTTTTAATCTAAATTCCTCCAATTTCGTATTACAATAAGTTGAAAAAAACCATAATCATGGAAATGCAAATGTTCTGATTTCTTTTCGATCGTCATGCATTGGTGTGGTACTGTGTAAAGGTGGATGACAAGTATCAATATTCATGTGACAACAAAGATAGCAGCGTCCATGGATGGATATCCACTGACCCGCCGGTCGGGTTCTGGCTAATCACCCCCAGCAACGAGTTCCGGTCCGGCGGACCCGTTAAGCAGAACCTGACTTCTCACGTCGGCCCAACCACCCTTGCTGTAAGTGATTCTTCATTCATTCTGCCTTCGTTCATGGGGTTGTTCAATGTTTGAGGGCATCAGAAGCCAGAGTCTTGACTCAAAACGACGTATCGAATGATCAGGTTTTTATTAGCGCCCATTACACGGGAGAGGATCTGGTCCCTAAAATCGGAGCGGGCGAGGCGTGGAAGAAAGTTTTTGGTCCTGTGTTCATCTATCTCAACTCAACTTACGATTCCAGCGACGCAATTCAGCTCTGGGAAGATGCCAAAGCACAGGTTTCTTCCTTTTTCTTCACTATTCATTTTCATTGGAAGATAGTTAATAACTACTCTACACTTCAGCTCTGGACTAAACACACACAACTCTAAAAGTTATTACTTAAAAATTTAATCTAGTTCTAAAATTTCACAACTTATTCAATCGAAGACTGAGACTTCTTGTTCCGTATGAAAAGGAGACGATCGAAGTCCAAAGTTGGCCCTACAGCTTCCCTGCTTCGGATGATTTTCCAAAGTCAAACCAGCGTGGTAATATCGGTGGTAGACTCTTAGTCAAGGATAGGTACACAATTGCCAATGGACATTGGAAAATCTTAATAGGTTTTAGTATTTAGTCCATAAATTAATTAATTAGTCGAATGAGGAGATTGACATGATTTAGGTACGTCAGCGAAGACTTCATACCAGCAAACGGTGCGTTTGTTGGCTTAGCGCCACCAGGAGACGTAGGATCGTGGCAAAGAGAGAGTAAGGTAAACTTATTTACTTTTCCTTATTTAATTTCCTTTTCTAAAAAGGGAATATTATTGGCTGATCATTTGAATATTATTAGGGCTACCAATTTTGGAGTAGAGCAGATGAAAGTGGATACTTTTCTATCAACGGCGTAAGAACTGGCGACTACAATCTTTATGCATGGGTCCCCGGTTTCATTGGAGATTATCGAAATGATTCTCTCATCAACATTTCAGAAGGTTCTAATTTTCTTCCTGTGGTTCACATTTATATATACTTTTTTGCAAGTCATGTAGCTTTGGACGTAAAATTGGTTAGAACAATTACTGGTTTAGGAATGCGAACGAATTAATCTATTAGATTTATAACAAATTAGAGATTTGGAACCAAATAAAATCTATCTAAACCAAATCGATCAATCATTTTTGAATTTATATGTTGTGAAATTGAATCGATTGAAATTTACTGGTAGGGTAGATGAACCAATTCAATTCTATCGATTTTATCTGTTTTTTCAATTTGAACTTGCAATTCTAAGTTAAAATTTTGTGTGTGCAAGAGTATAGGTAGTGAGATAGACGTGGGTGACATAATATACGAAGCTCCGAGAGAAGGGGCTACAATATGGGAAATAGGAATCCCCGATCGCTCAGCAGCAGAGTTCTATGTTCCAGATCCAAATCCTGGCTACGTCAATAAGCTCTATCTTAACCATCCTGATAAGTAAATATAAATCTACGAGCTCATCCCTCATTATTCTAATTAATCCCTTCATTTAATCAATTCGATTTATTCTTAAGTTGACAATTAATTTGGCAGGTTTCGGCAGTATGGTCTGTGGGAAAGATACACAGAATTGTACCCCGACGCAGACTTGGTATATACAATTGGCGTTAGTGATTACACTAAGGATTGGTTCTTCGCACAGGTCACCAGGTGTTTGTTTATCTTTAGTCTTAGTTTTTCTCTTCATCAATTCAATATTTAGATCTTAGGATTGGTTCTGAATTCTGATTTCTCTTCAAAAATACTATAAATTTTATTAATGTTCTAGTTTTTGCTGATCATGATCCGTAGGAAGACAGAAAACGGAACATTCAAGGGGACAACGTGGCAAGTGAAGTTCAAGCTGGACAATGTGGACAGAAAAGGAACTTACAAGCTTCGGATCGCTTTGGCTTCCGCTTCTCTGGCGGAATTGCAGGTATACAGATCAAATACAACCTTAATTATTTTCTTTTTTCCTTTCTAGTTTCTGGGGTGGCGTGCAAAACCAGTCCACTGTGATGTTTTTGAAGGTTGCCGTAAATGACCCAAAATCAAAGCCAGCGTTCTCGAGTGGGCTGATCGGAAGGGACAATTCGATCGCCAGACACGGAATTCACGGCCTGCATTGGCTCTACAACGTGGATATTCCGGCCAATCGCCTCGTCGCCGGGGACAACGTCATCTTTTTCACGCAACCCAGGAGCACCAGCCCTTTCCAAGCTATTATGTACGATTATATTCGTCTGGAATCTCCTCCACCTCCCTCCTCCGCCCATGCTTTGAAT

mRNA sequence

ATGCCGCCTCTGGGTGTGCAACTATACGTCACAGAACGTCAGGTACGTACGTCCCTCCCTCTCCTTTTCTCTATATTTCTTCTCCCCTTTATGTCAAAACGGAGAAATGTGCAGGTGGTGATGGATAATGGCATAGTTCAAGTCACGTTTTCAAACCCAGATGGCATAGTGACCGGAATCCGATACAATGGAGTCGACAACTTGCTCGAAATTCTCAACGACGAATATAACAGAGGGTATTGGGACCTCGTGTGGAGCCCATCCGCCTCCACAACTGGATCGTTCGATGTGATAAAGGCTACGAGTTTTGAGGTTATAGTGGAAAATGAAGACCAAGTGGAGCTCTCCTTTACACGAAAATGGGATCTGTCTCTCGAGGGAAAGCTTGTGCCTTTAGAGATTGATAAAAGGTTTGTAATGCTTCGGGGTTCGTCAGGGTTCTACTCTTATGCTATTTACCAACACTTGAAAGAATGGCCTGGTTTCAACCTTGGTGAAACCAGACTCGCCTTCAAACTCAGAAAAGACAAGTTCCACTATATGGCCATGGCAGACAACAGACAGAGATCCATGCCGCTGCCAGATGACCGGTCTCCGCCAAGAGGCCGAGCCCTGGCCTATCCGGAGGCTGTCCTGCTCGTCGACCCCGTCGAACCTGAGCTCCAGGGAGAGGTGGATGACAAGTATCAATATTCATGTGACAACAAAGATAGCAGCGTCCATGGATGGATATCCACTGACCCGCCGGTCGGGTTCTGGCTAATCACCCCCAGCAACGAGTTCCGGTCCGGCGGACCCGTTAAGCAGAACCTGACTTCTCACGTCGGCCCAACCACCCTTGCTGTTTTTATTAGCGCCCATTACACGGGAGAGGATCTGGTCCCTAAAATCGGAGCGGGCGAGGCGTGGAAGAAAGTTTTTGGTCCTGTGTTCATCTATCTCAACTCAACTTACGATTCCAGCGACGCAATTCAGCTCTGGGAAGATGCCAAAGCACAGGAGACGATCGAAGTCCAAAGTTGGCCCTACAGCTTCCCTGCTTCGGATGATTTTCCAAAGTCAAACCAGCGTGGTAATATCGGTGGTAGACTCTTAGTCAAGGATAGGTACGTCAGCGAAGACTTCATACCAGCAAACGGTGCGTTTGTTGGCTTAGCGCCACCAGGAGACGTAGGATCGTGGCAAAGAGAGAGTAAGGGCTACCAATTTTGGAGTAGAGCAGATGAAAGTGGATACTTTTCTATCAACGGCGTAAGAACTGGCGACTACAATCTTTATGCATGGGTCCCCGGTTTCATTGGAGATTATCGAAATGATTCTCTCATCAACATTTCAGAAGGTAGTGAGATAGACGTGGGTGACATAATATACGAAGCTCCGAGAGAAGGGGCTACAATATGGGAAATAGGAATCCCCGATCGCTCAGCAGCAGAGTTCTATGTTCCAGATCCAAATCCTGGCTACGTCAATAAGCTCTATCTTAACCATCCTGATAAGTTTCGGCAGTATGGTCTGTGGGAAAGATACACAGAATTGTACCCCGACGCAGACTTGGTATATACAATTGGCGTTAGTGATTACACTAAGGATTGGTTCTTCGCACAGGTCACCAGGAAGACAGAAAACGGAACATTCAAGGGGACAACGTGGCAAGTGAAGTTCAAGCTGGACAATGTGGACAGAAAAGGAACTTACAAGCTTCGGATCGCTTTGGCTTCCGCTTCTCTGGCGGAATTGCAGGTTGCCGTAAATGACCCAAAATCAAAGCCAGCGTTCTCGAGTGGGCTGATCGGAAGGGACAATTCGATCGCCAGACACGGAATTCACGGCCTGCATTGGCTCTACAACGTGGATATTCCGGCCAATCGCCTCGTCGCCGGGGACAACGTCATCTTTTTCACGCAACCCAGGAGCACCAGCCCTTTCCAAGCTATTATGTACGATTATATTCGTCTGGAATCTCCTCCACCTCCCTCCTCCGCCCATGCTTTGAAT

Coding sequence (CDS)

ATGCCGCCTCTGGGTGTGCAACTATACGTCACAGAACGTCAGGTACGTACGTCCCTCCCTCTCCTTTTCTCTATATTTCTTCTCCCCTTTATGTCAAAACGGAGAAATGTGCAGGTGGTGATGGATAATGGCATAGTTCAAGTCACGTTTTCAAACCCAGATGGCATAGTGACCGGAATCCGATACAATGGAGTCGACAACTTGCTCGAAATTCTCAACGACGAATATAACAGAGGGTATTGGGACCTCGTGTGGAGCCCATCCGCCTCCACAACTGGATCGTTCGATGTGATAAAGGCTACGAGTTTTGAGGTTATAGTGGAAAATGAAGACCAAGTGGAGCTCTCCTTTACACGAAAATGGGATCTGTCTCTCGAGGGAAAGCTTGTGCCTTTAGAGATTGATAAAAGGTTTGTAATGCTTCGGGGTTCGTCAGGGTTCTACTCTTATGCTATTTACCAACACTTGAAAGAATGGCCTGGTTTCAACCTTGGTGAAACCAGACTCGCCTTCAAACTCAGAAAAGACAAGTTCCACTATATGGCCATGGCAGACAACAGACAGAGATCCATGCCGCTGCCAGATGACCGGTCTCCGCCAAGAGGCCGAGCCCTGGCCTATCCGGAGGCTGTCCTGCTCGTCGACCCCGTCGAACCTGAGCTCCAGGGAGAGGTGGATGACAAGTATCAATATTCATGTGACAACAAAGATAGCAGCGTCCATGGATGGATATCCACTGACCCGCCGGTCGGGTTCTGGCTAATCACCCCCAGCAACGAGTTCCGGTCCGGCGGACCCGTTAAGCAGAACCTGACTTCTCACGTCGGCCCAACCACCCTTGCTGTTTTTATTAGCGCCCATTACACGGGAGAGGATCTGGTCCCTAAAATCGGAGCGGGCGAGGCGTGGAAGAAAGTTTTTGGTCCTGTGTTCATCTATCTCAACTCAACTTACGATTCCAGCGACGCAATTCAGCTCTGGGAAGATGCCAAAGCACAGGAGACGATCGAAGTCCAAAGTTGGCCCTACAGCTTCCCTGCTTCGGATGATTTTCCAAAGTCAAACCAGCGTGGTAATATCGGTGGTAGACTCTTAGTCAAGGATAGGTACGTCAGCGAAGACTTCATACCAGCAAACGGTGCGTTTGTTGGCTTAGCGCCACCAGGAGACGTAGGATCGTGGCAAAGAGAGAGTAAGGGCTACCAATTTTGGAGTAGAGCAGATGAAAGTGGATACTTTTCTATCAACGGCGTAAGAACTGGCGACTACAATCTTTATGCATGGGTCCCCGGTTTCATTGGAGATTATCGAAATGATTCTCTCATCAACATTTCAGAAGGTAGTGAGATAGACGTGGGTGACATAATATACGAAGCTCCGAGAGAAGGGGCTACAATATGGGAAATAGGAATCCCCGATCGCTCAGCAGCAGAGTTCTATGTTCCAGATCCAAATCCTGGCTACGTCAATAAGCTCTATCTTAACCATCCTGATAAGTTTCGGCAGTATGGTCTGTGGGAAAGATACACAGAATTGTACCCCGACGCAGACTTGGTATATACAATTGGCGTTAGTGATTACACTAAGGATTGGTTCTTCGCACAGGTCACCAGGAAGACAGAAAACGGAACATTCAAGGGGACAACGTGGCAAGTGAAGTTCAAGCTGGACAATGTGGACAGAAAAGGAACTTACAAGCTTCGGATCGCTTTGGCTTCCGCTTCTCTGGCGGAATTGCAGGTTGCCGTAAATGACCCAAAATCAAAGCCAGCGTTCTCGAGTGGGCTGATCGGAAGGGACAATTCGATCGCCAGACACGGAATTCACGGCCTGCATTGGCTCTACAACGTGGATATTCCGGCCAATCGCCTCGTCGCCGGGGACAACGTCATCTTTTTCACGCAACCCAGGAGCACCAGCCCTTTCCAAGCTATTATGTACGATTATATTCGTCTGGAATCTCCTCCACCTCCCTCCTCCGCCCATGCTTTGAAT

Protein sequence

MPPLGVQLYVTERQVRTSLPLLFSIFLLPFMSKRRNVQVVMDNGIVQVTFSNPDGIVTGIRYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRKWDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSVHGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNIGGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRTGDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATIWEIGIPDRSAAEFYVPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRDNSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSSAHALN

Homology

[Close]

BLAST of MS001133 vs. NCBI nr
Match: XP_022132072.1 (probable rhamnogalacturonate lyase B isoform X1 [Momordica charantia])

HSP 1 Score: 1320.4 bits (3416), Expect = 0.0e+00
Identity = 639/665 (96.09%), Postives = 640/665 (96.24%), Query Frame = 0


Query: 1   MPPLGVQLYVTERQVRTSLPLLFSIFLLPFMSKRRNVQVVMDNGIVQVTFSNPDGIVTGI 60
           MPPLGVQLYVTER                        QVVMDNGIVQVTFSNPDGIV GI
Sbjct: 76  MPPLGVQLYVTER------------------------QVVMDNGIVQVTFSNPDGIVIGI 135

Query: 61  RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK 120
           RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK
Sbjct: 136 RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK 195

Query: 121 WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 180
           WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY
Sbjct: 196 WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 255

Query: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV 240
           MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV
Sbjct: 256 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV 315

Query: 241 HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 300
           HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG
Sbjct: 316 HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 375

Query: 301 EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI 360
           EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI
Sbjct: 376 EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI 435

Query: 361 GGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT 420
           GGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT
Sbjct: 436 GGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT 495

Query: 421 GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATIWEIGIPDRSAAEFY 480
           GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGAT+WEIGIPDRSAAEFY
Sbjct: 496 GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATMWEIGIPDRSAAEFY 555

Query: 481 VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT 540
           VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT
Sbjct: 556 VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT 615

Query: 541 ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD 600
           ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD
Sbjct: 616 ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD 675

Query: 601 NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS 660
           NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS
Sbjct: 676 NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS 716

Query: 661 AHALN 666
           AHALN
Sbjct: 736 AHALN 716

[Close]

BLAST of MS001133 vs. NCBI nr
Match: XP_022132073.1 (probable rhamnogalacturonate lyase B isoform X2 [Momordica charantia])

HSP 1 Score: 1308.5 bits (3385), Expect = 0.0e+00
Identity = 636/665 (95.64%), Postives = 637/665 (95.79%), Query Frame = 0


Query: 1   MPPLGVQLYVTERQVRTSLPLLFSIFLLPFMSKRRNVQVVMDNGIVQVTFSNPDGIVTGI 60
           MPPLGVQLYVTER                        QVVMDNGIVQVTFSNPDGIV GI
Sbjct: 76  MPPLGVQLYVTER------------------------QVVMDNGIVQVTFSNPDGIVIGI 135

Query: 61  RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK 120
           RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK
Sbjct: 136 RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK 195

Query: 121 WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 180
           WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY
Sbjct: 196 WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 255

Query: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV 240
           MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV
Sbjct: 256 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV 315

Query: 241 HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 300
           HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG
Sbjct: 316 HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 375

Query: 301 EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI 360
           EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI
Sbjct: 376 EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI 435

Query: 361 GGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT 420
           GGRLLVKD   SEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT
Sbjct: 436 GGRLLVKD---SEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT 495

Query: 421 GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATIWEIGIPDRSAAEFY 480
           GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGAT+WEIGIPDRSAAEFY
Sbjct: 496 GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATMWEIGIPDRSAAEFY 555

Query: 481 VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT 540
           VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT
Sbjct: 556 VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT 615

Query: 541 ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD 600
           ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD
Sbjct: 616 ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD 675

Query: 601 NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS 660
           NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS
Sbjct: 676 NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS 713

Query: 661 AHALN 666
           AHALN
Sbjct: 736 AHALN 713

[Close]

BLAST of MS001133 vs. NCBI nr
Match: XP_023537007.1 (probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537008.1 probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537009.1 probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537010.1 probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1220.7 bits (3157), Expect = 0.0e+00
Identity = 579/664 (87.20%), Postives = 609/664 (91.72%), Query Frame = 0


Query: 1   MPPLGVQLYVTERQVRTSLPLLFSIFLLPFMSKRRNVQVVMDNGIVQVTFSNPDGIVTGI 60
           MPPLGVQL + E                         QV MDNGIVQVT SNPDGIVTGI
Sbjct: 1   MPPLGVQLSIAEH------------------------QVTMDNGIVQVTLSNPDGIVTGI 60

Query: 61  RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK 120
           RYNGVDNLLE+LNDE NRGYWDLVW+PSAS TGSFDVIKAT FEVIVENEDQVELSFTRK
Sbjct: 61  RYNGVDNLLEVLNDESNRGYWDLVWNPSASATGSFDVIKATDFEVIVENEDQVELSFTRK 120

Query: 121 WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 180
           WDLSLEGKLVPLEIDKRF+MLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKD+FHY
Sbjct: 121 WDLSLEGKLVPLEIDKRFIMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDRFHY 180

Query: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV 240
           MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDP+E ELQGEVDDKYQYSCDNKDS V
Sbjct: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPIESELQGEVDDKYQYSCDNKDSGV 240

Query: 241 HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 300
           HGWISTDPP+GFWLITPSNE RSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG
Sbjct: 241 HGWISTDPPIGFWLITPSNECRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 300

Query: 301 EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI 360
           EAWKKVFGPVFIYLNSTYDSSDA+QLWED K+Q +IEVQSWPYSFPAS+DFPKSNQRGNI
Sbjct: 301 EAWKKVFGPVFIYLNSTYDSSDALQLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNI 360

Query: 361 GGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT 420
            GRLLVKD YVSED+IPANGAFVGLAPPGDVGSWQRESKGYQFWSRADE GYF I+ VR 
Sbjct: 361 SGRLLVKDSYVSEDYIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADEDGYFLIDHVRP 420

Query: 421 GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATIWEIGIPDRSAAEFY 480
           GDYNLYAWVPGFIGDYR DS+INI+EGS+IDV +IIYE PR GAT+WEIG+PDRSAAEFY
Sbjct: 421 GDYNLYAWVPGFIGDYRYDSIINITEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAAEFY 480

Query: 481 VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT 540
           VPDPNP YVNKLYLNHPDKFRQYGLWERY+ELYPDADLVYT+GVSDYTKDWFFAQVTRKT
Sbjct: 481 VPDPNPNYVNKLYLNHPDKFRQYGLWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKT 540

Query: 541 ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD 600
           ENGTFKGTTWQVKF+LDNVD + TY+LR+ALASASLAELQV VNDPK+KP FSSGLIGRD
Sbjct: 541 ENGTFKGTTWQVKFELDNVDSQATYQLRVALASASLAELQVGVNDPKAKPVFSSGLIGRD 600

Query: 601 NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS 660
           NSIARHGIHGL+WLYNV+IP NRLV+GDN IFFTQPRSTSPFQAIMYDYIRLE+PPPPSS
Sbjct: 601 NSIARHGIHGLYWLYNVNIPGNRLVSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSS 640

Query: 661 AHAL 665
           AH L
Sbjct: 661 AHGL 640

[Close]

BLAST of MS001133 vs. NCBI nr
Match: XP_023001855.1 (rhamnogalacturonate lyase B-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1215.3 bits (3143), Expect = 0.0e+00
Identity = 577/664 (86.90%), Postives = 606/664 (91.27%), Query Frame = 0


Query: 1   MPPLGVQLYVTERQVRTSLPLLFSIFLLPFMSKRRNVQVVMDNGIVQVTFSNPDGIVTGI 60
           MPPLGVQL ++E                         QV MDNGIVQVT SNPDGIVTGI
Sbjct: 1   MPPLGVQLSISEH------------------------QVTMDNGIVQVTLSNPDGIVTGI 60

Query: 61  RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK 120
           RYNGVDNLLE+LNDE NRGYWDLVW+PSAS TGSFDVIKAT FEVIVENEDQVELSFTRK
Sbjct: 61  RYNGVDNLLEVLNDESNRGYWDLVWNPSASATGSFDVIKATDFEVIVENEDQVELSFTRK 120

Query: 121 WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 180
           WDLSLEGKLVPLEIDKRF+MLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY
Sbjct: 121 WDLSLEGKLVPLEIDKRFIMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 180

Query: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV 240
           MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDP+E ELQGEVDDKYQYSCDNKDS V
Sbjct: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPIESELQGEVDDKYQYSCDNKDSGV 240

Query: 241 HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 300
           HGWISTDPP+GFWLITPSNE RSGGPVKQNLTSHVGPT+LAVFISAHYTGEDLVPKIGAG
Sbjct: 241 HGWISTDPPIGFWLITPSNECRSGGPVKQNLTSHVGPTSLAVFISAHYTGEDLVPKIGAG 300

Query: 301 EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI 360
           EAWKKVFGPVFIYLNSTYD SDA QLWED K+Q +IEVQSWPYSFPAS+DFPKSNQRGNI
Sbjct: 301 EAWKKVFGPVFIYLNSTYDFSDAFQLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNI 360

Query: 361 GGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT 420
            GRLLVKD YVSED+IPANGAFVGLAPPGDVGSWQRESKGYQFWSRADE GYF I+ VR 
Sbjct: 361 SGRLLVKDSYVSEDYIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHVRP 420

Query: 421 GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATIWEIGIPDRSAAEFY 480
           GDYNLYAWVPGFIGDYR DS+INI EGS+IDV +IIYE PR GAT+WEIG+PDRSAAEFY
Sbjct: 421 GDYNLYAWVPGFIGDYRYDSIINIIEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAAEFY 480

Query: 481 VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT 540
           VPDPNP YVNKLYLNHPDKFRQYGLWERY+ELYPDADLVYT+GVSDYTKDWFFAQVTRKT
Sbjct: 481 VPDPNPNYVNKLYLNHPDKFRQYGLWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKT 540

Query: 541 ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD 600
           ENGTFKGTTWQVKF+LDNVD + TYKLR+ALASAS+ ELQV VNDPK+KP FSSGLIGRD
Sbjct: 541 ENGTFKGTTWQVKFELDNVDSQATYKLRVALASASIVELQVGVNDPKAKPLFSSGLIGRD 600

Query: 601 NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS 660
           NSIARHGIHGL+WLYNV+IP NRLV+GDN IFFTQPRSTSPFQAIMYDYIRLE+PPPPSS
Sbjct: 601 NSIARHGIHGLYWLYNVNIPGNRLVSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSS 640

Query: 661 AHAL 665
           AH L
Sbjct: 661 AHGL 640

[Close]

BLAST of MS001133 vs. NCBI nr
Match: KAG7020263.1 (rhiE [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1213.7 bits (3139), Expect = 0.0e+00
Identity = 576/662 (87.01%), Postives = 606/662 (91.54%), Query Frame = 0


Query: 1   MPPLGVQLYVTERQVRTSLPLLFSIFLLPFMSKRRNVQVVMDNGIVQVTFSNPDGIVTGI 60
           MPPLGVQL + E                         QV MDNGIVQVT SNPDGIVTGI
Sbjct: 1   MPPLGVQLSIAEH------------------------QVTMDNGIVQVTLSNPDGIVTGI 60

Query: 61  RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK 120
           RYNGVDNLLE+LNDE NRGYWDLVW+PSAS TGSFDVIKAT FEVIVENEDQVELSFTRK
Sbjct: 61  RYNGVDNLLEVLNDESNRGYWDLVWNPSASATGSFDVIKATDFEVIVENEDQVELSFTRK 120

Query: 121 WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 180
           WDLSLEGKLVPL IDKRF+MLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY
Sbjct: 121 WDLSLEGKLVPLLIDKRFIMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 180

Query: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV 240
           MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDP+E ELQGEVDDKYQYSCDNKDS V
Sbjct: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPIESELQGEVDDKYQYSCDNKDSGV 240

Query: 241 HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 300
           HGWISTDPP+GFWLITPSNE RSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG
Sbjct: 241 HGWISTDPPIGFWLITPSNECRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 300

Query: 301 EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI 360
           EAWKKVFGPVFIYLNSTYDSSDA+QLWED K+Q +IEVQSWPYSFPAS+DFPKSNQRGNI
Sbjct: 301 EAWKKVFGPVFIYLNSTYDSSDALQLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNI 360

Query: 361 GGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT 420
            G+LLVKD YVSED+IPANGAFVGLAPPGDVGSWQRESKGYQFWSRADE GYF I+ VR 
Sbjct: 361 SGKLLVKDSYVSEDYIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHVRP 420

Query: 421 GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATIWEIGIPDRSAAEFY 480
           GDYNLYAWVPGFIGDYR +S+INI EGS+IDV +IIYE PR GAT+WEIG+PDRSAAEFY
Sbjct: 421 GDYNLYAWVPGFIGDYRYNSIINILEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAAEFY 480

Query: 481 VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT 540
           VPDPNP YVNKLYLNHPDKFRQYGLWERY+ELYPDADLVYT+GVSDYTKDWFFAQVTRKT
Sbjct: 481 VPDPNPNYVNKLYLNHPDKFRQYGLWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKT 540

Query: 541 ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD 600
           ENGTFKGTTWQVKF+LDNVD + TYKLR+ALASAS+AELQV VNDPK+KP FSSGLIGRD
Sbjct: 541 ENGTFKGTTWQVKFELDNVDSQATYKLRVALASASIAELQVVVNDPKAKPVFSSGLIGRD 600

Query: 601 NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS 660
           NSIARHGIHGL+WLYNV+IP NRLV+GDN IFFTQPRSTSPFQAIMYDYIRLE+PPPPSS
Sbjct: 601 NSIARHGIHGLYWLYNVNIPGNRLVSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSS 638

Query: 661 AH 663
           AH
Sbjct: 661 AH 638

[Close]

BLAST of MS001133 vs. ExPASy Swiss-Prot
Match: A5ABH4 (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 3.1e-26
Identity = 163/702 (23.22%), Postives = 277/702 (39.46%), Query Frame = 0


Query: 21  LLFSIFLLPFMSKRRNVQVVMDNGIVQVTFSNPDGIVTGIRYNGVDNLLEILNDEYNRGY 80
           LL ++      + + N  + + N  +   FS   G +T +  +  D L     D     Y
Sbjct: 12  LLLTLTSATLHTSQTNTTITLTNNRLTANFSKSQGRITDLYLDNQDLLGPQSGDTGVGPY 71

Query: 81  WDLVWSPS-----ASTTGSFDVIKATSFEVIVENEDQVELSFTRKWDLSLEGKLVPL--E 140
            D    PS      ST+ +  +   T            + S T    + ++    P    
Sbjct: 72  LDCYCIPSGFYTPGSTSPTLQLFTGT------------DKSGTSYAGVLMDETYPPTGQH 131

Query: 141 IDKRFVMLRGSSGFYSYAIYQHLKEWPGF--NLGETRLAFKLRKDKFHYMAMADNRQRSM 200
             + + +  G +G ++++   +  E   +  NL E R  F+   + + +++ ++   ++ 
Sbjct: 132 FQQYWFLRDGETGLHTFSRLAYYNETTPYLRNLQEFRTLFRPNTELWTHLSSSE--VQTA 191

Query: 201 PLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDD----------KYQYSCDNKDSSVH 260
           PLP  +        A  E V++ D          D+          KY +S   +D+SVH
Sbjct: 192 PLPSKK--------AVEEEVVVQDATWTFNNTPTDEYYVQFADYFTKYTFSNAWRDNSVH 251

Query: 261 GW-----ISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPK 320
           G       S     G WL+  + +   GGP+  +LT  V        +S H+ GE   P 
Sbjct: 252 GMYADGSTSNGSTFGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNYLVSNHH-GEG-TPN 311

Query: 321 IGAGEAWKKVFGPVFIYLNSTYDSSDAIQ-LWEDAKAQETIEVQSWPYSFPAS-----DD 380
           I  G  + + FGP + + N    S+ ++Q L  DA   ET+   SW   F  S       
Sbjct: 312 ITYG--FDRTFGPQYYHFNGGKGSTASLQELKSDA---ETLADPSWNVDFYDSIAKHVVG 371

Query: 381 FPKSNQRGNIGGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRE----SKGYQFWSR 440
           +  S+QRG++ G++ +             GA   +A     G + ++    S  YQ+W+ 
Sbjct: 372 YTPSSQRGSVQGKIKL-----------PKGATRPIAVLTVDGQYFQDNSVNSSSYQYWAE 431

Query: 441 ADESGYFSINGVRTGDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATI 500
            D+SG+FS++ V+ G Y L  +  G  GD+  D  + +  G +  + +  +EA   G  I
Sbjct: 432 IDDSGHFSVDHVKEGPYRLTVYADGIFGDFVRDG-VQVKAGKKTTIQE-TWEAESAGTEI 491

Query: 501 WEIGIPDRSAAEF---YVPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIG 560
           W +G PD+S+ EF      DP         L+ P+    +G ++  ++ +PD  + YTIG
Sbjct: 492 WRLGTPDKSSGEFRHGVARDPTHP------LHPPEYLIYWGAYDWQSD-FPDG-INYTIG 551

Query: 561 VSDYTKD-----WFFAQVTRKTENGTFKGT-TWQVKFKL--DNVDRKGTYKLRIALASAS 620
            SD   D     W     T       +  T  W + F L  D++  +    L I LA A 
Sbjct: 552 TSDPATDLNTVHWSVFGPTPNDPRVEYDTTHDWTINFPLSEDDLAERSKATLTIQLAGAK 611

Query: 621 LAE----------------LQVAVNDPKSKPAFSSGLIGRDNSIARHGIHGLHWLYNVDI 659
            A                 L+  +ND         G     + I R  +        ++ 
Sbjct: 612 AASGNTDVYNASEPYTNLALESYINDQAEPLTLLIGFNQSSSCIVRSAVSCYQVRSRMEF 661

[Close]

BLAST of MS001133 vs. ExPASy Swiss-Prot
Match: Q5AZ85 (Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 8.5e-24
Identity = 157/691 (22.72%), Postives = 270/691 (39.07%), Query Frame = 0


Query: 18  SLPLLFSIFLLPFMSKRRNVQVVMDNGIVQVTFSNPDGIVTGIRYNGVDNLLEILNDEYN 77
           SL    S+      +   +  +V++N  ++ TF    G +  +  +G D L         
Sbjct: 8   SLAAAASVARAALTATENDTSIVLENDRLRATFDKGRGSIIDLYLDGQDFLGPQSGSTGI 67

Query: 78  RGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRKWDLSLEGKLVPL--EID 137
             Y D   +PS   T       A S   + E     + + T+   + L     P   E  
Sbjct: 68  GPYLDCYCTPSGFYT-------AGSTNPVTELVQGTDSTGTKYAGIILNDTYTPTGQEFQ 127

Query: 138 KRFVMLRGSSGFYSYAIYQHLKEWPGF--NLGETRLAFKLRKDKFHYMAMADNRQRSMPL 197
           + + +  G +GF+ ++   +  E   F  NL E R  F+   D + ++  +D   ++ PL
Sbjct: 128 QYWFLRDGETGFHMFSRLAYYNETTPFLRNLQELRTLFRPNTDLWTHLTSSD--LQTAPL 187

Query: 198 PDDRSPPRGRALAYPEAVLLVDPVEPELQ--GEVDDKYQYSCDNKDSSVHGW-----IST 257
           P D +      +      L   P +   Q   E   KY +S   +D+ VHG       S 
Sbjct: 188 PSDEAIAEQIVVQDATWRLNNTPDDAYYQQFSEYFTKYTFSNHWRDNDVHGLYADGSTSD 247

Query: 258 DPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKV 317
               G WL+  + +   GGP+  +LT  V        +S H+ GE   P I  G  + + 
Sbjct: 248 GTTYGAWLVMNTKDTYYGGPLHSDLT--VDGIIYNYIVSNHH-GEG-TPNITNG--FDRT 307

Query: 318 FGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPAS-----DDFPKSNQRGNIG 377
           FGP F YL +   SS   +L ++A+   ++   SW   F  S       +  S+QRG++ 
Sbjct: 308 FGPQF-YLFNGGGSSSLEELRDEAR---SLASPSWNADFYDSIAKHVIGYVPSSQRGSVK 367

Query: 378 GRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRES---KGYQFWSRADESGYFSINGV 437
           G +           +P N          D   +Q  S     +Q+W+  D++G FSI+ V
Sbjct: 368 GTIK----------LPKNAKSPIAVLTVDGHYFQDNSAVPSSHQYWADIDKNGRFSIDRV 427

Query: 438 RTGDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATIWEIGIPDRSAAE 497
             G Y L  +  G  GD+  D ++ +       + +  ++    G  IW +G PD+S+ E
Sbjct: 428 VAGKYRLTVYADGIFGDFTRDGIV-VKARKSTSIKE-TWKPESAGTEIWRLGTPDKSSGE 487

Query: 498 F---YVPDPNPGYVNKLYLNHPDKFRQY-GLWERYTELYPDADLVYTIGVSDYTKDW--- 557
           F      DP        +  HP ++  Y G ++  ++     D  Y IG SD   D+   
Sbjct: 488 FRHGAARDPT-------HPRHPPEYLIYWGAYDWQSDFPGGID--YMIGESDPATDFNTV 547

Query: 558 FFAQVTRKTENGTFKGTT---WQVKFKLD--NVDRKGTYKLRIALASASLAE-------- 617
            +A      +N   +  T   W+++F L    +  + T  L I LA A  A         
Sbjct: 548 HWAVFGPTPDNPVAESNTTHDWRIRFDLSAKQLHARKTATLTIQLAGAKAASGNTDVYNA 607

Query: 618 --------LQVAVNDPKSKPAFSSGLIGRDNSIARHGIHGLHWLYNVDIPANRLVAGDNV 654
                   L+  +N+ +       G     + I R  +         + PA+ L  G N+
Sbjct: 608 SEPYANLPLRSYINEQEEPLTMVIGYDQSSSCIVRSAVSCYQVREKWEFPASWLKEGSNL 658

[Close]

BLAST of MS001133 vs. ExPASy Swiss-Prot
Match: Q2U5P7 (Probable rhamnogalacturonate lyase C OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rglC PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.1e-10
Identity = 107/469 (22.81%), Postives = 172/469 (36.67%), Query Frame = 0


Query: 137 RFVMLRG-SSGFYSYAIYQHLKE-WPGF-NLGETRLAFKLRKDKFHYMAMADNRQRSMPL 196
           ++  LRG  +G ++++   +  E  P   +LGE R  F+   D + + + +D      PL
Sbjct: 123 QWFFLRGEETGLHAFSRVTYFNETTPSLRSLGELRTLFRPSTDLWTHFSTSDGNYGPKPL 182

Query: 197 PDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSVHGWI------STD 256
             +       A  Y   V   D        +   KY  +   ++  VHG        S  
Sbjct: 183 GSNSGLVVQDATTYIGNV--TDDPYVSQYSDYFTKYTLAESWRNHDVHGLFSDGSSSSDG 242

Query: 257 PPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAGEAWKKVF 316
              G WL+  + E   GGP+  +L   V        +S H+      P       + + +
Sbjct: 243 STFGAWLVHNTVETYYGGPLHSDLV--VDGIVYNYLVSGHHG----APTPNLTHGFDRTW 302

Query: 317 GPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPAS--DDFPKSNQRGNIGGRLL 376
           GP F Y N     +    L  DA      E  +  Y   A    +F  S  R    G++ 
Sbjct: 303 GPQFYYFNRGDSETTLADLRADAAKYADPEWNAEFYDSIADHIPNFTPSTGRTTFKGKVS 362

Query: 377 VKDRYVSEDFIPANGAFVGLAPPG-DVGSWQRESKGYQFWSRADESGYFSINGVRTGDYN 436
           +           A    + L+  G D       ++  Q+W+  D+SG FSI  V  G Y 
Sbjct: 363 LPK--------GAKRPIIVLSEDGQDFQLNVFNTESLQYWAEIDKSGSFSIPRVVEGTYR 422

Query: 437 LYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATIWEIGIPDRSAAEF---YV 496
           +  +     G +  D  + + +    D     ++    G  IW IGIPD+S+ E+   Y 
Sbjct: 423 ITIYADEIFGWFIQDH-VKVLKSQSKDY-SFTWKEESAGKEIWRIGIPDKSSGEYLHGYA 482

Query: 497 PDPNPGYVNKLYLNHPDKFRQYGLWERYTELYP---DADLVYTIGVSDYTKD-----WFF 556
           PD +           P++ R Y  W +Y   YP      + + +G SD ++D     W F
Sbjct: 483 PDTSKPL-------QPEQHRIY--WGKYD--YPADFPEGINFHVGKSDPSQDLNYIHWAF 542

Query: 557 AQVT---RKTENGTFKGTTWQVKFKL--DNVDRKGTYKLRIALASASLA 578
                   +TE        W V F L  D +    T    + +A A  A
Sbjct: 543 FPSQGNHLRTEPYYDNVNNWTVTFDLTADQLHNTNTATFTVQIAGAKTA 562

[Close]

BLAST of MS001133 vs. ExPASy TrEMBL
Match: A0A6J1BSU2 (Rhamnogalacturonan endolyase OS=Momordica charantia OX=3673 GN=LOC111005034 PE=3 SV=1)

HSP 1 Score: 1320.4 bits (3416), Expect = 0.0e+00
Identity = 639/665 (96.09%), Postives = 640/665 (96.24%), Query Frame = 0


Query: 1   MPPLGVQLYVTERQVRTSLPLLFSIFLLPFMSKRRNVQVVMDNGIVQVTFSNPDGIVTGI 60
           MPPLGVQLYVTER                        QVVMDNGIVQVTFSNPDGIV GI
Sbjct: 76  MPPLGVQLYVTER------------------------QVVMDNGIVQVTFSNPDGIVIGI 135

Query: 61  RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK 120
           RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK
Sbjct: 136 RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK 195

Query: 121 WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 180
           WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY
Sbjct: 196 WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 255

Query: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV 240
           MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV
Sbjct: 256 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV 315

Query: 241 HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 300
           HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG
Sbjct: 316 HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 375

Query: 301 EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI 360
           EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI
Sbjct: 376 EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI 435

Query: 361 GGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT 420
           GGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT
Sbjct: 436 GGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT 495

Query: 421 GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATIWEIGIPDRSAAEFY 480
           GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGAT+WEIGIPDRSAAEFY
Sbjct: 496 GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATMWEIGIPDRSAAEFY 555

Query: 481 VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT 540
           VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT
Sbjct: 556 VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT 615

Query: 541 ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD 600
           ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD
Sbjct: 616 ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD 675

Query: 601 NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS 660
           NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS
Sbjct: 676 NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS 716

Query: 661 AHALN 666
           AHALN
Sbjct: 736 AHALN 716

[Close]

BLAST of MS001133 vs. ExPASy TrEMBL
Match: A0A6J1BV89 (Rhamnogalacturonan endolyase OS=Momordica charantia OX=3673 GN=LOC111005034 PE=3 SV=1)

HSP 1 Score: 1308.5 bits (3385), Expect = 0.0e+00
Identity = 636/665 (95.64%), Postives = 637/665 (95.79%), Query Frame = 0


Query: 1   MPPLGVQLYVTERQVRTSLPLLFSIFLLPFMSKRRNVQVVMDNGIVQVTFSNPDGIVTGI 60
           MPPLGVQLYVTER                        QVVMDNGIVQVTFSNPDGIV GI
Sbjct: 76  MPPLGVQLYVTER------------------------QVVMDNGIVQVTFSNPDGIVIGI 135

Query: 61  RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK 120
           RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK
Sbjct: 136 RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK 195

Query: 121 WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 180
           WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY
Sbjct: 196 WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 255

Query: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV 240
           MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV
Sbjct: 256 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV 315

Query: 241 HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 300
           HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG
Sbjct: 316 HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 375

Query: 301 EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI 360
           EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI
Sbjct: 376 EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI 435

Query: 361 GGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT 420
           GGRLLVKD   SEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT
Sbjct: 436 GGRLLVKD---SEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT 495

Query: 421 GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATIWEIGIPDRSAAEFY 480
           GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGAT+WEIGIPDRSAAEFY
Sbjct: 496 GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATMWEIGIPDRSAAEFY 555

Query: 481 VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT 540
           VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT
Sbjct: 556 VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT 615

Query: 541 ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD 600
           ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD
Sbjct: 616 ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD 675

Query: 601 NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS 660
           NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS
Sbjct: 676 NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS 713

Query: 661 AHALN 666
           AHALN
Sbjct: 736 AHALN 713

[Close]

BLAST of MS001133 vs. ExPASy TrEMBL
Match: A0A6J1KMB3 (Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111495919 PE=3 SV=1)

HSP 1 Score: 1215.3 bits (3143), Expect = 0.0e+00
Identity = 577/664 (86.90%), Postives = 606/664 (91.27%), Query Frame = 0


Query: 1   MPPLGVQLYVTERQVRTSLPLLFSIFLLPFMSKRRNVQVVMDNGIVQVTFSNPDGIVTGI 60
           MPPLGVQL ++E                         QV MDNGIVQVT SNPDGIVTGI
Sbjct: 1   MPPLGVQLSISEH------------------------QVTMDNGIVQVTLSNPDGIVTGI 60

Query: 61  RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK 120
           RYNGVDNLLE+LNDE NRGYWDLVW+PSAS TGSFDVIKAT FEVIVENEDQVELSFTRK
Sbjct: 61  RYNGVDNLLEVLNDESNRGYWDLVWNPSASATGSFDVIKATDFEVIVENEDQVELSFTRK 120

Query: 121 WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 180
           WDLSLEGKLVPLEIDKRF+MLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY
Sbjct: 121 WDLSLEGKLVPLEIDKRFIMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 180

Query: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV 240
           MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDP+E ELQGEVDDKYQYSCDNKDS V
Sbjct: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPIESELQGEVDDKYQYSCDNKDSGV 240

Query: 241 HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 300
           HGWISTDPP+GFWLITPSNE RSGGPVKQNLTSHVGPT+LAVFISAHYTGEDLVPKIGAG
Sbjct: 241 HGWISTDPPIGFWLITPSNECRSGGPVKQNLTSHVGPTSLAVFISAHYTGEDLVPKIGAG 300

Query: 301 EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI 360
           EAWKKVFGPVFIYLNSTYD SDA QLWED K+Q +IEVQSWPYSFPAS+DFPKSNQRGNI
Sbjct: 301 EAWKKVFGPVFIYLNSTYDFSDAFQLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNI 360

Query: 361 GGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT 420
            GRLLVKD YVSED+IPANGAFVGLAPPGDVGSWQRESKGYQFWSRADE GYF I+ VR 
Sbjct: 361 SGRLLVKDSYVSEDYIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHVRP 420

Query: 421 GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATIWEIGIPDRSAAEFY 480
           GDYNLYAWVPGFIGDYR DS+INI EGS+IDV +IIYE PR GAT+WEIG+PDRSAAEFY
Sbjct: 421 GDYNLYAWVPGFIGDYRYDSIINIIEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAAEFY 480

Query: 481 VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT 540
           VPDPNP YVNKLYLNHPDKFRQYGLWERY+ELYPDADLVYT+GVSDYTKDWFFAQVTRKT
Sbjct: 481 VPDPNPNYVNKLYLNHPDKFRQYGLWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKT 540

Query: 541 ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD 600
           ENGTFKGTTWQVKF+LDNVD + TYKLR+ALASAS+ ELQV VNDPK+KP FSSGLIGRD
Sbjct: 541 ENGTFKGTTWQVKFELDNVDSQATYKLRVALASASIVELQVGVNDPKAKPLFSSGLIGRD 600

Query: 601 NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS 660
           NSIARHGIHGL+WLYNV+IP NRLV+GDN IFFTQPRSTSPFQAIMYDYIRLE+PPPPSS
Sbjct: 601 NSIARHGIHGLYWLYNVNIPGNRLVSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSS 640

Query: 661 AHAL 665
           AH L
Sbjct: 661 AHGL 640

[Close]

BLAST of MS001133 vs. ExPASy TrEMBL
Match: A0A6J1GGG5 (Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111454003 PE=3 SV=1)

HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 574/664 (86.45%), Postives = 605/664 (91.11%), Query Frame = 0


Query: 1   MPPLGVQLYVTERQVRTSLPLLFSIFLLPFMSKRRNVQVVMDNGIVQVTFSNPDGIVTGI 60
           MPPLGVQL + E                         QV MDNGIVQVT SNPDGIVTGI
Sbjct: 1   MPPLGVQLSIAEH------------------------QVTMDNGIVQVTLSNPDGIVTGI 60

Query: 61  RYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKATSFEVIVENEDQVELSFTRK 120
           RYNGVDNLLE+LNDE NRGYWDLVW+PSAS TGSFDVIKAT FEVIVENEDQVELSFTRK
Sbjct: 61  RYNGVDNLLEVLNDESNRGYWDLVWNPSASATGSFDVIKATDFEVIVENEDQVELSFTRK 120

Query: 121 WDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 180
           WDLSLEGKLVPL IDKRF+MLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY
Sbjct: 121 WDLSLEGKLVPLLIDKRFIMLRGSSGFYSYAIYQHLKEWPGFNLGETRLAFKLRKDKFHY 180

Query: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKYQYSCDNKDSSV 240
           MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDP+E EL+GEVDDKYQYSCDNKDS V
Sbjct: 181 MAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPIESELKGEVDDKYQYSCDNKDSGV 240

Query: 241 HGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 300
           HGWISTDPP+GFWLITPSNE RSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG
Sbjct: 241 HGWISTDPPIGFWLITPSNECRSGGPVKQNLTSHVGPTTLAVFISAHYTGEDLVPKIGAG 300

Query: 301 EAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASDDFPKSNQRGNI 360
           EAWKKVFGPVFIYLNSTYDSSDA+QLWED K+Q +IEVQSWPYSFPAS+DFPKSNQRGNI
Sbjct: 301 EAWKKVFGPVFIYLNSTYDSSDALQLWEDGKSQASIEVQSWPYSFPASEDFPKSNQRGNI 360

Query: 361 GGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADESGYFSINGVRT 420
            GRLLVKD YVSED+IPANGAFVGLAPPGDVGSWQRESKGYQFWSRADE GYF I+ +R 
Sbjct: 361 SGRLLVKDSYVSEDYIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADEDGYFFIDHIRP 420

Query: 421 GDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATIWEIGIPDRSAAEFY 480
           GDYNLYAWVPGFIGDYR DS+I+I EGS+IDV +IIYE PR GAT+WEIG+PDRSA EFY
Sbjct: 421 GDYNLYAWVPGFIGDYRYDSIIDILEGSQIDVDEIIYEPPRAGATLWEIGVPDRSAEEFY 480

Query: 481 VPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTKDWFFAQVTRKT 540
           VPDPNP YVNKLYLNH DKFRQYGLWERY+ELYPDADLVYT+GVSDYTKDWFFAQVTRKT
Sbjct: 481 VPDPNPNYVNKLYLNHSDKFRQYGLWERYSELYPDADLVYTVGVSDYTKDWFFAQVTRKT 540

Query: 541 ENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSKPAFSSGLIGRD 600
           ENGTFKGTTWQVKF+LDNVD + TYKLR+ALASAS+AELQV VNDPK+KP FSSGLIGRD
Sbjct: 541 ENGTFKGTTWQVKFELDNVDSQATYKLRVALASASIAELQVGVNDPKAKPVFSSGLIGRD 600

Query: 601 NSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDYIRLESPPPPSS 660
           NSIARHGIHGL+WLYNV+IP NRLV+GDN IFFTQPRSTSPFQAIMYDYIRLE+PPPPSS
Sbjct: 601 NSIARHGIHGLYWLYNVNIPGNRLVSGDNTIFFTQPRSTSPFQAIMYDYIRLEAPPPPSS 640

Query: 661 AHAL 665
           AH L
Sbjct: 661 AHGL 640

[Close]

BLAST of MS001133 vs. ExPASy TrEMBL
Match: A0A6J1KHT0 (Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111495919 PE=3 SV=1)

HSP 1 Score: 1206.8 bits (3121), Expect = 0.0e+00
Identity = 577/675 (85.48%), Postives = 606/675 (89.78%), Query Frame = 0


Query: 1   MPPLGVQLYVTERQVRTSLPLLFSIFLLPFMSKRRNVQVVMDNGIVQVTFSNPDGIVTGI 60
           MPPLGVQL ++E                         QV MDNGIVQVT SNPDGIVTGI
Sbjct: 1   MPPLGVQLSISEH------------------------QVTMDNGIVQVTLSNPDGIVTGI 60

Query: 61  RYNGVDNLLEILNDEYNRG-----------YWDLVWSPSASTTGSFDVIKATSFEVIVEN 120
           RYNGVDNLLE+LNDE NRG           YWDLVW+PSAS TGSFDVIKAT FEVIVEN
Sbjct: 61  RYNGVDNLLEVLNDESNRGYIMNEKLASYKYWDLVWNPSASATGSFDVIKATDFEVIVEN 120

Query: 121 EDQVELSFTRKWDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWPGFNLGETRL 180
           EDQVELSFTRKWDLSLEGKLVPLEIDKRF+MLRGSSGFYSYAIYQHLKEWPGFNLGETRL
Sbjct: 121 EDQVELSFTRKWDLSLEGKLVPLEIDKRFIMLRGSSGFYSYAIYQHLKEWPGFNLGETRL 180

Query: 181 AFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPELQGEVDDKY 240
           AFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDP+E ELQGEVDDKY
Sbjct: 181 AFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPIESELQGEVDDKY 240

Query: 241 QYSCDNKDSSVHGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFISAHYT 300
           QYSCDNKDS VHGWISTDPP+GFWLITPSNE RSGGPVKQNLTSHVGPT+LAVFISAHYT
Sbjct: 241 QYSCDNKDSGVHGWISTDPPIGFWLITPSNECRSGGPVKQNLTSHVGPTSLAVFISAHYT 300

Query: 301 GEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEVQSWPYSFPASD 360
           GEDLVPKIGAGEAWKKVFGPVFIYLNSTYD SDA QLWED K+Q +IEVQSWPYSFPAS+
Sbjct: 301 GEDLVPKIGAGEAWKKVFGPVFIYLNSTYDFSDAFQLWEDGKSQASIEVQSWPYSFPASE 360

Query: 361 DFPKSNQRGNIGGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADE 420
           DFPKSNQRGNI GRLLVKD YVSED+IPANGAFVGLAPPGDVGSWQRESKGYQFWSRADE
Sbjct: 361 DFPKSNQRGNISGRLLVKDSYVSEDYIPANGAFVGLAPPGDVGSWQRESKGYQFWSRADE 420

Query: 421 SGYFSINGVRTGDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYEAPREGATIWEI 480
            GYF I+ VR GDYNLYAWVPGFIGDYR DS+INI EGS+IDV +IIYE PR GAT+WEI
Sbjct: 421 DGYFFIDHVRPGDYNLYAWVPGFIGDYRYDSIINIIEGSQIDVDEIIYEPPRAGATLWEI 480

Query: 481 GIPDRSAAEFYVPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADLVYTIGVSDYTK 540
           G+PDRSAAEFYVPDPNP YVNKLYLNHPDKFRQYGLWERY+ELYPDADLVYT+GVSDYTK
Sbjct: 481 GVPDRSAAEFYVPDPNPNYVNKLYLNHPDKFRQYGLWERYSELYPDADLVYTVGVSDYTK 540

Query: 541 DWFFAQVTRKTENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAELQVAVNDPKSK 600
           DWFFAQVTRKTENGTFKGTTWQVKF+LDNVD + TYKLR+ALASAS+ ELQV VNDPK+K
Sbjct: 541 DWFFAQVTRKTENGTFKGTTWQVKFELDNVDSQATYKLRVALASASIVELQVGVNDPKAK 600

Query: 601 PAFSSGLIGRDNSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRSTSPFQAIMYDY 660
           P FSSGLIGRDNSIARHGIHGL+WLYNV+IP NRLV+GDN IFFTQPRSTSPFQAIMYDY
Sbjct: 601 PLFSSGLIGRDNSIARHGIHGLYWLYNVNIPGNRLVSGDNTIFFTQPRSTSPFQAIMYDY 651

Query: 661 IRLESPPPPSSAHAL 665
           IRLE+PPPPSSAH L
Sbjct: 661 IRLEAPPPPSSAHGL 651

[Close]

BLAST of MS001133 vs. TAIR 10
Match: AT1G09890.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 964.5 bits (2492), Expect = 4.3e-281
Identity = 438/617 (70.99%), Postives = 520/617 (84.28%), Query Frame = 0


Query: 41  MDNGIVQVTFSNPDGIVTGIRYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVIKA 100
           MDNGI +VT S PDGIVTGI YNG+DNLLE+LN+E NRGYWDLVW  S  T G FDVIK 
Sbjct: 1   MDNGIARVTLSKPDGIVTGIEYNGIDNLLEVLNEEVNRGYWDLVWGGS-GTAGGFDVIKG 60

Query: 101 TSFEVIVENEDQVELSFTRKWDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKEWP 160
           ++FEVIV+NE+Q+ELSFTRKWD S EGK VPL IDKRFVML GSSGFY+YAIY+HLKEWP
Sbjct: 61  SNFEVIVKNEEQIELSFTRKWDPSQEGKAVPLNIDKRFVMLSGSSGFYTYAIYEHLKEWP 120

Query: 161 GFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVEPE 220
            F+L ETR+AFKLRK+KFHYMA+ D+RQR MPLPDDR P RG+ALAYPEAVLLV+P+E +
Sbjct: 121 AFSLAETRIAFKLRKEKFHYMAVTDDRQRFMPLPDDRLPDRGQALAYPEAVLLVNPLESQ 180

Query: 221 LQGEVDDKYQYSCDNKDSSVHGWISTD-PPVGFWLITPSNEFRSGGPVKQNLTSHVGPTT 280
            +GEVDDKYQYSC+NKD +VHGWI T+ P VGFWLITPS+E+R+GGP KQNLTSHVGPT 
Sbjct: 181 FKGEVDDKYQYSCENKDITVHGWICTEQPSVGFWLITPSHEYRTGGPQKQNLTSHVGPTA 240

Query: 281 LAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLN-STYDSSDAIQLWEDAKAQETIEV 340
           LAVFISAHYTGEDLVPK   GEAWKKVFGPVF+YLN ST D +D + LW+DAK+Q  +E 
Sbjct: 241 LAVFISAHYTGEDLVPKFSEGEAWKKVFGPVFVYLNSSTDDDNDPLWLWQDAKSQMNVEA 300

Query: 341 QSWPYSFPASDDFPKSNQRGNIGGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRES 400
           +SWPYSFPASDD+ K+ QRGN+ GRLLV+DRYV +DFI AN  +VGLA PG  GSWQRE 
Sbjct: 301 ESWPYSFPASDDYVKTEQRGNVVGRLLVQDRYVDKDFIAANRGYVGLAVPGAAGSWQREC 360

Query: 401 KGYQFWSRADESGYFSINGVRTGDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYE 460
           K YQFW+R DE G+F I+G+R G YNLYAW+PGFIGDY+ D +I I+ G  I V D++Y+
Sbjct: 361 KEYQFWTRTDEEGFFYISGIRPGQYNLYAWIPGFIGDYKYDDVITITSGCYIYVEDLVYQ 420

Query: 461 APREGATIWEIGIPDRSAAEFYVPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADL 520
            PR GAT+WEIG PDRSAAEFYVPDPNP Y+N LY NHPD+FRQYGLWERY ELYPD DL
Sbjct: 421 PPRNGATLWEIGFPDRSAAEFYVPDPNPKYINNLYQNHPDRFRQYGLWERYAELYPDKDL 480

Query: 521 VYTIGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAE 580
           VY +G SDY KDWF+AQVTRK +N T++GTTWQ+KF+L N+D+  +Y LR+A+ASA+ +E
Sbjct: 481 VYVVGSSDYRKDWFYAQVTRKKDNKTYQGTTWQIKFELKNIDKNHSYTLRVAIASATFSE 540

Query: 581 LQVAVNDPKSKPAFSSGLIGRDNSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPRS 640
           LQ+ VN+  + P F+SGLIGRDNSIARHGIHGL+WL+NV++  ++L+ G+N +F TQPRS
Sbjct: 541 LQIRVNNANASPMFTSGLIGRDNSIARHGIHGLYWLFNVEVAGSKLLEGENTLFLTQPRS 600

Query: 641 TSPFQAIMYDYIRLESP 656
           TSPFQ IMYDYIR E+P
Sbjct: 601 TSPFQGIMYDYIRFEAP 616

[Close]

BLAST of MS001133 vs. TAIR 10
Match: AT1G09910.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 897.1 bits (2317), Expect = 8.4e-261
Identity = 408/619 (65.91%), Postives = 495/619 (79.97%), Query Frame = 0


Query: 39  VVMDNGIVQVTFSNPDGIVTGIRYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVI 98
           VVMDNGI+QVT S P GI+TGI YNG+DN+LE+ N E NRGYWDL W+      G FDVI
Sbjct: 58  VVMDNGILQVTLSKPGGIITGIEYNGIDNVLEVRNKETNRGYWDLHWNEPGG-KGIFDVI 117

Query: 99  KATSFEVIVENEDQVELSFTRKWDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKE 158
              +F VIVE E+QVE+SF R WD SLEGK +PL IDKRF+MLRGSSG YSY IY+HLK+
Sbjct: 118 SGVTFRVIVETEEQVEISFLRTWDPSLEGKYIPLNIDKRFIMLRGSSGVYSYGIYEHLKD 177

Query: 159 WPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVE 218
           WPGF LGETR+AFKLRKDKFHYMA+AD+R+R MP PDD    R + L Y EA LL  P +
Sbjct: 178 WPGFELGETRIAFKLRKDKFHYMAVADDRKRIMPFPDDLCKGRCQTLDYQEASLLTAPCD 237

Query: 219 PELQGEVDDKYQYSCDNKDSSVHGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPT 278
           P LQGEVDDKYQYSC+NKD  VHGWIS DPPVGFW ITPSNEFRSGGP+KQNLTSHVGPT
Sbjct: 238 PRLQGEVDDKYQYSCENKDLRVHGWISFDPPVGFWQITPSNEFRSGGPLKQNLTSHVGPT 297

Query: 279 TLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEV 338
           TLAVF S HY G+ ++P+   GE WKKV+GPVFIYLNST +  D + LW+DAK +   EV
Sbjct: 298 TLAVFHSTHYAGKTMMPRFEHGEPWKKVYGPVFIYLNSTANGDDPLCLWDDAKIKMMAEV 357

Query: 339 QSWPYSFPASDDFPKSNQRGNIGGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRES 398
           + WPYSF ASDD+PKS +RG   GRLL++DR+++ D I A GA+VGLAPPGD GSWQ E 
Sbjct: 358 ERWPYSFVASDDYPKSEERGTARGRLLIRDRFINNDLISARGAYVGLAPPGDSGSWQIEC 417

Query: 399 KGYQFWSRADESGYFSINGVRTGDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYE 458
           KGYQFW+ ADE+GYFSI  VR G+YNLYAWVP FIGDY N +++ ++ G  I++GDI+YE
Sbjct: 418 KGYQFWAIADEAGYFSIGNVRPGEYNLYAWVPSFIGDYHNGTIVRVTSGCMIEMGDIVYE 477

Query: 459 APREGATIWEIGIPDRSAAEFYVPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPDADL 518
            PR+G T+WEIGIPDR A+EF++PDP+P  VN++ ++H D+FRQYGLW++YT++YP+ DL
Sbjct: 478 PPRDGPTLWEIGIPDRKASEFFIPDPDPTLVNRVLVHHQDRFRQYGLWKKYTDMYPNDDL 537

Query: 519 VYTIGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLAE 578
           VYT+GVSDY +DWFFA V RK +    +GTTWQ+ F L+N+D+K  YKLR+A+ASA+LAE
Sbjct: 538 VYTVGVSDYRRDWFFAHVPRK-KGDVHEGTTWQIIFNLENIDQKANYKLRVAIASATLAE 597

Query: 579 LQVAVNDPKS-KPAFSSGLIGRDNSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPR 638
           LQ+ +ND ++ +P F++GLIGRDNSIARHGIHG++ LY V+IP NRLV GDN IF  QPR
Sbjct: 598 LQIRINDAEAIRPLFTTGLIGRDNSIARHGIHGVYMLYAVNIPGNRLVQGDNTIFLKQPR 657

Query: 639 STSPFQAIMYDYIRLESPP 657
              PFQ IMYDYIRLE PP
Sbjct: 658 CNGPFQGIMYDYIRLEGPP 674

[Close]

BLAST of MS001133 vs. TAIR 10
Match: AT4G24430.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 892.1 bits (2304), Expect = 2.7e-259
Identity = 416/631 (65.93%), Postives = 509/631 (80.67%), Query Frame = 0


Query: 39  VVMDNGIVQVTFSNPDGIVTGIRYNGVDNLLEILNDEYNRGYWDLVWSPSAS--TTGSFD 98
           VVM NG V+VT S PDG VTGI Y GVDNLLE  N+++NRGYWDLVWS   +  TTG  +
Sbjct: 16  VVMGNGKVKVTISKPDGFVTGISYQGVDNLLETHNEDFNRGYWDLVWSDEGTPGTTGKSE 75

Query: 99  VIKATSFEVIVENEDQVELSFTRKWDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHL 158
            IK TSFEV+VENE+ VE+SF+RKWD SL+  + P+ +DKRF+M +  +GFYSYAI++HL
Sbjct: 76  RIKGTSFEVVVENEELVEISFSRKWDSSLQDSIAPINVDKRFIMRKDVTGFYSYAIFEHL 135

Query: 159 KEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDP 218
            EWP FNL +TR+ +KLRKDKF YMA+ADNRQR MPLP+DR   RGR LAYPEAVLLV P
Sbjct: 136 AEWPAFNLPQTRIVYKLRKDKFKYMAIADNRQRKMPLPEDRLGKRGRPLAYPEAVLLVHP 195

Query: 219 VEPELQGEVDDKYQYSCDNKDSSVHGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVG 278
           VE E +GEVDDKY+YS +NKD  VHGWIS +  +G W I PSNEFRSGG  KQNLTSHVG
Sbjct: 196 VEDEFKGEVDDKYEYSSENKDLKVHGWISHNLDLGCWQIIPSNEFRSGGLSKQNLTSHVG 255

Query: 279 PTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYD-SSDAIQLWEDAKAQET 338
           P +LA+F+SAHY GED+V K+ AG++WKKVFGPVF YLN   D +SD + LW+DAK Q  
Sbjct: 256 PISLAMFLSAHYAGEDMVMKVKAGDSWKKVFGPVFTYLNCLPDKTSDPLSLWQDAKNQML 315

Query: 339 IEVQSWPYSFPASDDFPKSNQRGNIGGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQ 398
            EVQSWPY FPAS+DFP S++RG I GRLLV D+++S+DF+PANGAFVGLAPPG+VGSWQ
Sbjct: 316 TEVQSWPYDFPASEDFPVSDKRGCISGRLLVCDKFLSDDFLPANGAFVGLAPPGEVGSWQ 375

Query: 399 RESKGYQFWSRADESGYFSINGVRTGDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDI 458
            ESKGYQFW+ AD  GYF+IN +R G+YNL  +V G+IGDY+ + LINI+ G +IDVG+I
Sbjct: 376 LESKGYQFWTEADSDGYFAINDIREGEYNLNGYVTGWIGDYQYEQLINITAGCDIDVGNI 435

Query: 459 IYEAPREGATIWEIGIPDRSAAEFYVPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPD 518
           +YE PR+G T+WEIGIPDRSAAEF+VPDPNP Y+NKLY+ HPD+FRQYGLWERYTELYP 
Sbjct: 436 VYEPPRDGPTVWEIGIPDRSAAEFFVPDPNPKYINKLYIGHPDRFRQYGLWERYTELYPK 495

Query: 519 ADLVYTIGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASAS 578
            DLV+TIGVSDY KDWFFA VTRK  + T++ TTWQ+KFKL+NV +  TYK+RIALA+A+
Sbjct: 496 EDLVFTIGVSDYKKDWFFAHVTRKMGDDTYQKTTWQIKFKLENVQKSCTYKIRIALATAN 555

Query: 579 LAELQVAVND---PKSKPAFSSGLIGRDNSIARHGIHGLHWLYNVDIPANRLVAGDNVIF 638
           +AELQV +ND    K+ P F++G+IG DN+IARHGIHG++ LYNVD+P+ +LV GDN +F
Sbjct: 556 VAELQVRMNDDDTEKTTPIFTTGVIGHDNAIARHGIHGIYRLYNVDVPSEKLVEGDNTLF 615

Query: 639 FTQPRSTS-PFQAIMYDYIRLESPPPPSSAH 663
            TQ  +T+  F  +MYDYIRLE PP  S +H
Sbjct: 616 LTQTMTTTGAFNGLMYDYIRLEGPPLDSYSH 646

[Close]

BLAST of MS001133 vs. TAIR 10
Match: AT1G09880.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 853.6 bits (2204), Expect = 1.1e-247
Identity = 395/623 (63.40%), Postives = 491/623 (78.81%), Query Frame = 0


Query: 41  MDNGIVQVTFSNPDGIVTGIRYNGVDNLLE-ILNDEYNRGYWDLVWS----PSASTTGSF 100
           M+N  +Q+T SNP+G VTGI+YNG+DN+L    N EY+RGYWD+VW+     +  T G+ 
Sbjct: 1   MENRFLQLTLSNPEGFVTGIQYNGIDNVLAYYTNKEYDRGYWDVVWNFPGKKAKKTKGTL 60

Query: 101 DVIKATSFEVIVENEDQVELSFTRKWDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQH 160
           D I+AT  EVI +N++++ELSFTR W+ S     VP+ IDKRFVML+ SSGFYSYAI++ 
Sbjct: 61  DRIEATKMEVITQNDEKIELSFTRTWNTS-STTAVPVNIDKRFVMLQNSSGFYSYAIFER 120

Query: 161 LKEWPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVD 220
           L+ WP   L   RL FKL K KFHYMA++D+RQR MP+PDDR PPRG+ LAYPEAV L+D
Sbjct: 121 LQGWPAVELDNMRLVFKLNKKKFHYMAISDDRQRYMPVPDDRVPPRGQPLAYPEAVQLLD 180

Query: 221 PVEPELQGEVDDKYQYSCDNKDSSVHGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHV 280
           P+EPE +GEVDDKY+YS ++KD  VHGWIST+  VGFW ITPSNEFRS GP+KQ L SHV
Sbjct: 181 PIEPEFKGEVDDKYEYSMESKDIKVHGWISTNDSVGFWQITPSNEFRSAGPLKQFLGSHV 240

Query: 281 GPTTLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQET 340
           GPT LAVF S HY G DL+     GEAWKKVFGPVFIYLNS     D + LW +AK Q  
Sbjct: 241 GPTNLAVFHSTHYVGADLIMSFKNGEAWKKVFGPVFIYLNSFPKGVDPLLLWHEAKNQTK 300

Query: 341 IEVQSWPYSFPASDDFPKSNQRGNIGGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQ 400
           IE + WPY+F ASDDFP S+QRG++ GRLLV+DR++S + IPANG++VGLA PGDVGSWQ
Sbjct: 301 IEEEKWPYNFTASDDFPASDQRGSVSGRLLVRDRFISSEDIPANGSYVGLAAPGDVGSWQ 360

Query: 401 RESKGYQFWSRADESGYFSINGVRTGDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDI 460
           RE KGYQFWS+ADE+G FSIN VR+G YNLYA+ PGFIGDY ND++ +IS GS+I +GD+
Sbjct: 361 RECKGYQFWSKADENGSFSINNVRSGRYNLYAFAPGFIGDYHNDTVFDISPGSKISLGDL 420

Query: 461 IYEAPREGATIWEIGIPDRSAAEFYVPDPNPGYVNKLYLNHPDKFRQYGLWERYTELYPD 520
           +YE PR+G+T+WEIG+PDRSAAEFY+PDPNP +VNKLYLNH DK+RQYGLWERY+ELYPD
Sbjct: 421 VYEPPRDGSTLWEIGVPDRSAAEFYIPDPNPSFVNKLYLNHSDKYRQYGLWERYSELYPD 480

Query: 521 ADLVYTIGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFKLDNVDRK--GTYKLRIALAS 580
            D+VY + + DY+K+WFF QVTRK  NG +KGTTWQ++F+ D+  +   G +KLRIALA+
Sbjct: 481 EDMVYNVDIDDYSKNWFFMQVTRKQANGGYKGTTWQIRFQFDDKMKNVTGNFKLRIALAT 540

Query: 581 ASLAELQVAVND-PKSKPAFSSGLIGRDNSIARHGIHGLHWLYNVDIPANRLVAGDNVIF 640
           +++AELQV VND     P F +  IGRDN+IARHGIHGL+WLY+V++PA  L  G+N I+
Sbjct: 541 SNVAELQVRVNDLSADPPLFRTEQIGRDNTIARHGIHGLYWLYSVNVPAASLHVGNNTIY 600

Query: 641 FTQPRSTSPFQAIMYDYIRLESP 656
            TQ  +TSPFQ +MYDYIRLE P
Sbjct: 601 LTQALATSPFQGLMYDYIRLEYP 622

[Close]

BLAST of MS001133 vs. TAIR 10
Match: AT2G22620.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 765.8 bits (1976), Expect = 2.9e-221
Identity = 355/618 (57.44%), Postives = 454/618 (73.46%), Query Frame = 0


Query: 39  VVMDNGIVQVTFSNPDGIVTGIRYNGVDNLLEILNDEYNRGYWDLVWSPSASTTGSFDVI 98
           VV+DNGIVQVTFSNP+G++TGI+Y+G+DN+L+   D+  RGYWD+VW        + D +
Sbjct: 62  VVVDNGIVQVTFSNPEGLITGIKYHGIDNVLDDKIDD--RGYWDVVWYEPEKKQKT-DKL 121

Query: 99  KATSFEVIVENEDQVELSFTRKWDLSLEGKLVPLEIDKRFVMLRGSSGFYSYAIYQHLKE 158
           + T FE+I +NE+Q+E+SFTR W +S  G LVPL +DKR+++  G SG Y Y I + L+ 
Sbjct: 122 EGTKFEIITQNEEQIEISFTRTWTISRRGSLVPLNVDKRYIIRSGVSGLYMYGILERLEG 181

Query: 159 WPGFNLGETRLAFKLRKDKFHYMAMADNRQRSMPLPDDRSPPRGRALAYPEAVLLVDPVE 218
           WP  ++ + R+ FKL   KF +MA++D+RQRSMP   DR     ++LAY EAVLL +P  
Sbjct: 182 WPDVDMDQIRIVFKLNPKKFDFMAISDDRQRSMPSMADRE--NSKSLAYKEAVLLTNPSN 241

Query: 219 PELQGEVDDKYQYSCDNKDSSVHGWISTDPPVGFWLITPSNEFRSGGPVKQNLTSHVGPT 278
           P  +GEVDDKY YS ++KD++VHGWIS+DPPVGFW+ITPS+EFR GGP+KQ+LTSH GP 
Sbjct: 242 PMFKGEVDDKYMYSMEDKDNNVHGWISSDPPVGFWMITPSDEFRLGGPIKQDLTSHAGPI 301

Query: 279 TLAVFISAHYTGEDLVPKIGAGEAWKKVFGPVFIYLNSTYDSSDAIQLWEDAKAQETIEV 338
           TL++F S HY G+++      GE WKKVFGPV  YLNS       ++LW DAK Q   EV
Sbjct: 302 TLSMFTSTHYAGKEMRMDYRNGEPWKKVFGPVLAYLNSVSPKDSTLRLWRDAKRQMAAEV 361

Query: 339 QSWPYSFPASDDFPKSNQRGNIGGRLLVKDRYVSEDFIPANGAFVGLAPPGDVGSWQRES 398
           +SWPY F  S+D+P  +QRG + G+ L+KD YVS   I    AFVGLAP G+ GSWQ ES
Sbjct: 362 KSWPYDFITSEDYPLRHQRGTLEGQFLIKDSYVSRLKIYGKFAFVGLAPIGEAGSWQTES 421

Query: 399 KGYQFWSRADESGYFSINGVRTGDYNLYAWVPGFIGDYRNDSLINISEGSEIDVGDIIYE 458
           KGYQFW++AD  G F I  VR G+Y+LYAW  GFIGDY+ +  I I+ GSE++VG ++YE
Sbjct: 422 KGYQFWTKADRRGRFIIENVRAGNYSLYAWGSGFIGDYKYEQNITITPGSEMNVGPLVYE 481

Query: 459 APREGATIWEIGIPDRSAAEFYVPDPNPGYVNKLYLNH-PDKFRQYGLWERYTELYPDAD 518
            PR G T+WEIG+PDR+A EFY+PDP P  +NKLY+N   D+FRQYGLW+RY +LYP  D
Sbjct: 482 PPRNGPTLWEIGVPDRTAGEFYIPDPYPTLMNKLYVNPLQDRFRQYGLWDRYADLYPQND 541

Query: 519 LVYTIGVSDYTKDWFFAQVTRKTENGTFKGTTWQVKFKLDNVDRKGTYKLRIALASASLA 578
           LVYTIGVSDY  DWFFA V R   N T++ TTWQ+ F L NV+R G Y LRIALASA+ +
Sbjct: 542 LVYTIGVSDYRSDWFFAHVARNVGNDTYQPTTWQIIFNLKNVNRIGRYTLRIALASAADS 601

Query: 579 ELQVAVNDPKSKPAFSSGLIGRDNSIARHGIHGLHWLYNVDIPANRLVAGDNVIFFTQPR 638
           ELQ+ +NDPKS   F++G IG+DN+IARHGIHGL+ LY++D+  N L  GDN IF TQ R
Sbjct: 602 ELQIRINDPKSDAIFTTGFIGKDNAIARHGIHGLYRLYSIDVAGNLLSVGDNTIFLTQTR 661

Query: 639 STSPFQAIMYDYIRLESP 656
           S +PFQ IMYDYIRLESP
Sbjct: 662 SRTPFQGIMYDYIRLESP 674

The following BLAST results are available for this feature:

BLAST of MS001133 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132072.1	0.0e+00	96.09	probable rhamnogalacturonate lyase B isoform X1 [Momordica charantia]	[more]
XP_022132073.1	0.0e+00	95.64	probable rhamnogalacturonate lyase B isoform X2 [Momordica charantia]	[more]
XP_023537007.1	0.0e+00	87.20	probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo] >XP...	[more]
XP_023001855.1	0.0e+00	86.90	rhamnogalacturonate lyase B-like isoform X2 [Cucurbita maxima]	[more]
KAG7020263.1	0.0e+00	87.01	rhiE [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MS001133 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A5ABH4	3.1e-26	23.22	Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / F...	[more]
Q5AZ85	8.5e-24	22.72	Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 ...	[more]
Q2U5P7	1.1e-10	22.81	Probable rhamnogalacturonate lyase C OS=Aspergillus oryzae (strain ATCC 42149 / ...	[more]

BLAST of MS001133 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BSU2	0.0e+00	96.09	Rhamnogalacturonan endolyase OS=Momordica charantia OX=3673 GN=LOC111005034 PE=3...	[more]
A0A6J1BV89	0.0e+00	95.64	Rhamnogalacturonan endolyase OS=Momordica charantia OX=3673 GN=LOC111005034 PE=3...	[more]
A0A6J1KMB3	0.0e+00	86.90	Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111495919 PE=3 SV...	[more]
A0A6J1GGG5	0.0e+00	86.45	Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111454003 PE=3 ...	[more]
A0A6J1KHT0	0.0e+00	85.48	Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111495919 PE=3 SV...	[more]

BLAST of MS001133 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G09890.1	4.3e-281	70.99	Rhamnogalacturonate lyase family protein	[more]
AT1G09910.1	8.4e-261	65.91	Rhamnogalacturonate lyase family protein	[more]
AT4G24430.1	2.7e-259	65.93	Rhamnogalacturonate lyase family protein	[more]
AT1G09880.1	1.1e-247	63.40	Rhamnogalacturonate lyase family protein	[more]
AT2G22620.1	2.9e-221	57.44	Rhamnogalacturonate lyase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029411	Rhamnogalacturonan lyase, domain III	PFAM	PF14683	CBM-like	coord: 465..652 e-value: 7.7E-58 score: 195.1
IPR010325	Rhamnogalacturonate lyase	PFAM	PF06045	Rhamnogal_lyase	coord: 35..225 e-value: 1.7E-90 score: 302.2
None	No IPR available	GENE3D	2.60.120.260		coord: 462..653 e-value: 3.5E-23 score: 84.0
None	No IPR available	GENE3D	2.60.40.1120		coord: 354..452 e-value: 3.3E-6 score: 29.2
None	No IPR available	PANTHER	PTHR32018:SF8	RHAMNOGALACTURONATE LYASE FAMILY PROTEIN	coord: 35..656
None	No IPR available	PANTHER	PTHR32018	RHAMNOGALACTURONATE LYASE FAMILY PROTEIN	coord: 35..656
None	No IPR available	CDD	cd10320	RGL4_N	coord: 36..323 e-value: 4.36278E-82 score: 258.473
None	No IPR available	CDD	cd10317	RGL4_C	coord: 467..653 e-value: 3.44126E-57 score: 189.024
IPR029413	Rhamnogalacturonan lyase, domain II	PFAM	PF14686	fn3_3	coord: 379..451 e-value: 5.1E-25 score: 87.1
IPR029413	Rhamnogalacturonan lyase, domain II	CDD	cd10316	RGL4_M	coord: 356..455 e-value: 1.93237E-33 score: 121.202
IPR014718	Glycoside hydrolase-type carbohydrate-binding	GENE3D	2.70.98.10		coord: 30..327 e-value: 2.3E-11 score: 45.6
IPR008979	Galactose-binding-like domain superfamily	SUPERFAMILY	49785	Galactose-binding domain-like	coord: 463..653
IPR011013	Galactose mutarotase-like domain superfamily	SUPERFAMILY	74650	Galactose mutarotase-like	coord: 43..317
IPR013784	Carbohydrate-binding-like fold	SUPERFAMILY	49452	Starch-binding domain-like	coord: 377..454

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001133.1	MS001133.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0003824	catalytic activity