MS000928 (gene) Bitter gourd (TR) v1

Overview
NameMS000928
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionsufE-like protein 2, chloroplastic
Locationscaffold36: 316524 .. 317231 (-)
RNA-Seq ExpressionMS000928
SyntenyMS000928
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTCAATCCCTATAATTACTTCTTGTGGTTTTGGCTTCGCCGAGTTTTCAGATTCATCCAAGCTTTCTTTTGAACGAATTCGCTTTCGTCAAGTAGAAAACACGAGATTTCTCGCATCCAAATCTGTAAGATGCGCTATATCTCATGATCTTAATGTCACTTCCTCCAATAAATTATGTAGTCGACGCCCTAAGTGCGATCTCGATGCGATTACAATTCCGGTTTATGCGTCTGAGTTAGTGGCCGATAAGATTCATCGGCTTGTTCTGGAATTCGAGTCTCTTTTGGAGCCGATTGACCGGGTGAAACGACTGTTGCATTACGCAGCGATTCTTGATCCTTCGGACGAATCGATTCGACTGCCGGAGAATCGAGTCAAGGGATGCGCGACGCAGGTGTGGTTGGATGTGAAAATTGATGAGTTCGAGCGAATGAGATTTAAAGCGGATAGCGATTCAGAGATCGCGAAGGGATATTGTTCCTGCTTGATCTGGATGTTGGATGGCGCGGAGCCATTGGAGGTGTTGAAGGTGAGGAGCGATGATTTGGACGCTGTGAATGTTGGATTGCACGGAAAAGCGATATCTAGGGTGAATACATGGCAAAATGTGTTGATGAGTATGCAAATGAGGACCAGCGCTCTGGTCTCTAGAAGTGAACCGAAGCCGCCATTGGAGCCTTTTATCATCACCGTTCATCATCGC

mRNA sequence

ATGAGTTCAATCCCTATAATTACTTCTTGTGGTTTTGGCTTCGCCGAGTTTTCAGATTCATCCAAGCTTTCTTTTGAACGAATTCGCTTTCGTCAAGTAGAAAACACGAGATTTCTCGCATCCAAATCTGTAAGATGCGCTATATCTCATGATCTTAATGTCACTTCCTCCAATAAATTATGTAGTCGACGCCCTAAGTGCGATCTCGATGCGATTACAATTCCGGTTTATGCGTCTGAGTTAGTGGCCGATAAGATTCATCGGCTTGTTCTGGAATTCGAGTCTCTTTTGGAGCCGATTGACCGGGTGAAACGACTGTTGCATTACGCAGCGATTCTTGATCCTTCGGACGAATCGATTCGACTGCCGGAGAATCGAGTCAAGGGATGCGCGACGCAGGTGTGGTTGGATGTGAAAATTGATGAGTTCGAGCGAATGAGATTTAAAGCGGATAGCGATTCAGAGATCGCGAAGGGATATTGTTCCTGCTTGATCTGGATGTTGGATGGCGCGGAGCCATTGGAGGTGTTGAAGGTGAGGAGCGATGATTTGGACGCTGTGAATGTTGGATTGCACGGAAAAGCGATATCTAGGGTGAATACATGGCAAAATGTGTTGATGAGTATGCAAATGAGGACCAGCGCTCTGGTCTCTAGAAGTGAACCGAAGCCGCCATTGGAGCCTTTTATCATCACCGTTCATCATCGC

Coding sequence (CDS)

ATGAGTTCAATCCCTATAATTACTTCTTGTGGTTTTGGCTTCGCCGAGTTTTCAGATTCATCCAAGCTTTCTTTTGAACGAATTCGCTTTCGTCAAGTAGAAAACACGAGATTTCTCGCATCCAAATCTGTAAGATGCGCTATATCTCATGATCTTAATGTCACTTCCTCCAATAAATTATGTAGTCGACGCCCTAAGTGCGATCTCGATGCGATTACAATTCCGGTTTATGCGTCTGAGTTAGTGGCCGATAAGATTCATCGGCTTGTTCTGGAATTCGAGTCTCTTTTGGAGCCGATTGACCGGGTGAAACGACTGTTGCATTACGCAGCGATTCTTGATCCTTCGGACGAATCGATTCGACTGCCGGAGAATCGAGTCAAGGGATGCGCGACGCAGGTGTGGTTGGATGTGAAAATTGATGAGTTCGAGCGAATGAGATTTAAAGCGGATAGCGATTCAGAGATCGCGAAGGGATATTGTTCCTGCTTGATCTGGATGTTGGATGGCGCGGAGCCATTGGAGGTGTTGAAGGTGAGGAGCGATGATTTGGACGCTGTGAATGTTGGATTGCACGGAAAAGCGATATCTAGGGTGAATACATGGCAAAATGTGTTGATGAGTATGCAAATGAGGACCAGCGCTCTGGTCTCTAGAAGTGAACCGAAGCCGCCATTGGAGCCTTTTATCATCACCGTTCATCATCGC

Protein sequence

MSSIPIITSCGFGFAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKLCSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESIRLPENRVKGCATQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVRSDDLDAVNVGLHGKAISRVNTWQNVLMSMQMRTSALVSRSEPKPPLEPFIITVHHR
Homology
BLAST of MS000928 vs. NCBI nr
Match: XP_022131476.1 (sufE-like protein 2, chloroplastic [Momordica charantia])

HSP 1 Score: 469.2 bits (1206), Expect = 2.2e-128
Identity = 233/236 (98.73%), Postives = 235/236 (99.58%), Query Frame = 0

Query: 1   MSSIPIITSCGFGFAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 60
           MSSIPIITSCGFGFAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL
Sbjct: 1   MSSIPIITSCGFGFAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 60

Query: 61  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 120
           CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI
Sbjct: 61  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 120

Query: 121 RLPENRVKGCATQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 180
           RLPENRVKGC TQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR
Sbjct: 121 RLPENRVKGCXTQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 180

Query: 181 SDDLDAVNVGLHGKAISRVNTWQNVLMSMQMRTSALVSRSEPKPPLEPFIITVHHR 237
           SDDLDAVNVGLHGKAISRVNTWQNV+MSMQMRT+ALVSRSEPKPPLEPFIITVHHR
Sbjct: 181 SDDLDAVNVGLHGKAISRVNTWQNVMMSMQMRTNALVSRSEPKPPLEPFIITVHHR 236

BLAST of MS000928 vs. NCBI nr
Match: XP_038886328.1 (sufE-like protein 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 391.3 bits (1004), Expect = 5.7e-105
Identity = 194/237 (81.86%), Postives = 211/237 (89.03%), Query Frame = 0

Query: 1   MSSIPIITSCGFGFAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 60
           MSSIP+ITS G GFA+FS SS L  ++I    + N RF+ASKSV C ISHDLN+TSSNKL
Sbjct: 29  MSSIPMITSSGIGFAKFSGSSNLCLQQIPSPHMLNRRFVASKSVTCTISHDLNLTSSNKL 88

Query: 61  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 120
           C+RRP CDLD+I IP+Y SELVAD++HRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI
Sbjct: 89  CTRRPNCDLDSIAIPLYTSELVADRLHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 148

Query: 121 RLPENRVKGCATQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 180
           RLPENRV+GCATQVWLDVKIDEF+RMRFKADSDSEIAKGYCSCLIWML+GAEP EVLKVR
Sbjct: 149 RLPENRVRGCATQVWLDVKIDEFDRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVR 208

Query: 181 SDDLDAVNVGLHGKAISRVNTWQNVLMSMQMRTSALVSRSEPKPPLEPFII-TVHHR 237
           SDDL+AVNVGLHGKA SRVNTWQNVLMSMQMRT  LVS+ E KPPLEPF   TVHHR
Sbjct: 209 SDDLEAVNVGLHGKAASRVNTWQNVLMSMQMRTKTLVSKIEAKPPLEPFTFRTVHHR 265

BLAST of MS000928 vs. NCBI nr
Match: KAA0061053.1 (sufE-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 372.5 bits (955), Expect = 2.7e-99
Identity = 187/237 (78.90%), Postives = 206/237 (86.92%), Query Frame = 0

Query: 1   MSSIPIITSCGFGFAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 60
           MSSIP+I S    F++FS SSKL F++ R   V N RF ASKSVRCAISHDLN+TSSNKL
Sbjct: 1   MSSIPMIASSRIAFSDFSGSSKLHFQQFRSPHVVNRRFAASKSVRCAISHDLNLTSSNKL 60

Query: 61  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 120
           CS RP CDLD+I IPVY+SELVADK+ RLVLEFESLLEPIDRVKRLLHYAAIL  SD++I
Sbjct: 61  CSPRPNCDLDSIAIPVYSSELVADKLRRLVLEFESLLEPIDRVKRLLHYAAILGSSDKAI 120

Query: 121 RLPENRVKGCATQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 180
           R+PENRV+GCA QVWLDVKIDEF+RMRFKADSDSEIAKGYCSCLIWML+GAEP EVL VR
Sbjct: 121 RIPENRVRGCAAQVWLDVKIDEFDRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLNVR 180

Query: 181 SDDLDAVNVGLHGKAISRVNTWQNVLMSMQMRTSALVSRSEPKPPLEPFII-TVHHR 237
           SDDL+AVNVGLHGKA SRVNTWQNVLMSMQMRT+ LVS++E KP LEPF   TVH R
Sbjct: 181 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSKTEVKPLLEPFTFRTVHRR 237

BLAST of MS000928 vs. NCBI nr
Match: XP_008444379.1 (PREDICTED: sufE-like protein 2, chloroplastic [Cucumis melo])

HSP 1 Score: 364.4 bits (934), Expect = 7.5e-97
Identity = 185/237 (78.06%), Postives = 202/237 (85.23%), Query Frame = 0

Query: 1   MSSIPIITSCGFGFAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 60
           MSSIP+I S    F++FS SSKL F++ R   V N RF ASKSVRCAISHDLN+TSSNKL
Sbjct: 1   MSSIPMIASSRIAFSDFSGSSKLHFQQFRSPHVVNRRFAASKSVRCAISHDLNLTSSNKL 60

Query: 61  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 120
           CS RP CDLD+I IPVY+SELVADK+ RLVLEFESLLEPIDRVKRLLHYAAIL  SD++I
Sbjct: 61  CSPRPNCDLDSIAIPVYSSELVADKLRRLVLEFESLLEPIDRVKRLLHYAAILGSSDKAI 120

Query: 121 RLPENRVKGCATQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 180
           R+PENRV+GCA QVWLDVKIDEF+RMRFKADSDSEIAKGYCSCLIWML+GAEP EVL VR
Sbjct: 121 RIPENRVRGCAAQVWLDVKIDEFDRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLNVR 180

Query: 181 SDDLDAVNVGLHGKAISRVNTWQNVLMSMQMRTSALVSRSEPKPPLEPFII-TVHHR 237
           SDDL+AVNVGLHGKA SRVNTWQNVLMSMQMRT+ L      KPPLEPF   TVH R
Sbjct: 181 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTL------KPPLEPFTFRTVHRR 231

BLAST of MS000928 vs. NCBI nr
Match: XP_004143058.1 (sufE-like protein 2, chloroplastic [Cucumis sativus] >KGN62329.1 hypothetical protein Csa_018584 [Cucumis sativus])

HSP 1 Score: 339.3 bits (869), Expect = 2.6e-89
Identity = 177/237 (74.68%), Postives = 192/237 (81.01%), Query Frame = 0

Query: 2   SSIPIITSCGFG-FAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 61
           S IP+  S   G F +FS SS L F++ RF ++ N    AS      ISH LN+TSSNKL
Sbjct: 3   SIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAAS------ISHHLNLTSSNKL 62

Query: 62  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 121
           CS RP CDL +I +PVY SE VADK+ RLVLEFESLLEPIDRVKRLLHYAAILD SDE+I
Sbjct: 63  CSPRPNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAI 122

Query: 122 RLPENRVKGCATQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 181
           RLPENRVKGCA QVWLDV +DEF RMRFKADSDSEIAKGYCSCLIWML+GAEP EVLKVR
Sbjct: 123 RLPENRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVR 182

Query: 182 SDDLDAVNVGLHGKAISRVNTWQNVLMSMQMRTSALVSRSEPKPPLEPFII-TVHHR 237
           SDDL+AVNVGLHGKA SRVNTWQNVLMSMQMRT+ LVS SE KPPLEPF   TVH R
Sbjct: 183 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRR 233

BLAST of MS000928 vs. ExPASy Swiss-Prot
Match: Q9FXE3 (SufE-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SUFE2 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 9.9e-52
Identity = 111/208 (53.37%), Postives = 138/208 (66.35%), Query Frame = 0

Query: 14  FAEFSDSSKLSFERIRFRQVENTRF---LASKSVRCAISHDLNVTSSNKLCSRRPKCDLD 73
           F   +    +S  R   +   N RF    + K + C +   LN+ S+ K  S      L 
Sbjct: 7   FKALASPPLISTSRPTTKSFPNPRFTSRFSPKPITC-MRDSLNLGSNPKAPS---PFSLA 66

Query: 74  AITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESIRLPENRVKGC 133
            +++        +DK+  LV EF SL EPIDRVKRLL+YAA L P DES R+ ENRV GC
Sbjct: 67  TVSVDAPLGTKTSDKLRILVSEFRSLTEPIDRVKRLLNYAATLAPLDESARISENRVTGC 126

Query: 134 ATQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVRSDDLDAVNVG 193
            TQVWL++K+DEF RMRFKADSDSEI+KG+CSCLIW+LDGA+P EV+ VRS+DL  +NVG
Sbjct: 127 TTQVWLEIKMDEFGRMRFKADSDSEISKGFCSCLIWILDGAKPEEVMGVRSEDLSEMNVG 186

Query: 194 LHGKAISRVNTWQNVLMSMQMRTSALVS 219
           +HGK  SRVNTW NVLMSMQ RT  LV+
Sbjct: 187 VHGKEQSRVNTWHNVLMSMQKRTMTLVA 210

BLAST of MS000928 vs. ExPASy Swiss-Prot
Match: Q9FGS4 (Quinolinate synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=QS PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 8.7e-40
Identity = 84/160 (52.50%), Postives = 114/160 (71.25%), Query Frame = 0

Query: 79  SELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESIRLPENRVKGCATQVWLDV 138
           +ELV  K+ RLV EF+SL EPIDR+K +LHYA++L    ES +   NRV GC  +VWLD 
Sbjct: 81  TELVPYKLQRLVKEFKSLTEPIDRLKWVLHYASLLPQMPESSKTESNRVMGCTARVWLDA 140

Query: 139 KIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVRSDDLDAVNVGLHGKAISR 198
           ++ +  +MRF ADSDS+++KG CSCLI +LD A P+EV++++++DL  +NVGL G   SR
Sbjct: 141 ELGQDGKMRFCADSDSDVSKGMCSCLIQVLDEASPVEVMELKTEDLAELNVGLLGGERSR 200

Query: 199 VNTWQNVLMSMQMRTSALVSRSEPK-PPLEPF---IITVH 235
           VNTW NVL+SMQ +T  LV+  E K P  EPF   ++T H
Sbjct: 201 VNTWYNVLVSMQKKTRRLVAEREGKVPSFEPFPSLVLTAH 240

BLAST of MS000928 vs. ExPASy Swiss-Prot
Match: Q2QTL0 (Quinolinate synthase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0290150 PE=3 SV=2)

HSP 1 Score: 122.1 bits (305), Expect = 8.5e-27
Identity = 68/146 (46.58%), Postives = 86/146 (58.90%), Query Frame = 0

Query: 85  KIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESIRLPENRVKGCATQVWLDVKIDEFE 144
           ++ RL  EF    +  DR +RLL  AA L    E+ R+  NRV GC  QVWL  + D   
Sbjct: 69  RVSRLAEEFRVSPDAADRARRLLARAAALPRLGEADRVAANRVMGCVAQVWLVGRCDGAG 128

Query: 145 RMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVRSDDLDAVNVGLHGK-AISRVNTWQ 204
           RMRF ADSDSE+++GYC+CL+  LDGA P EVL V   DL  +     G  A SR +TW 
Sbjct: 129 RMRFAADSDSELSRGYCACLVSALDGARPEEVLDVDPADLAPLGGAAAGTGARSRASTWH 188

Query: 205 NVLMSMQMRTSALVSRSEPKPPLEPF 230
           NVL+ MQ R  A ++  E +P  EPF
Sbjct: 189 NVLIGMQKRARAAIAAREGRPAGEPF 214

BLAST of MS000928 vs. ExPASy Swiss-Prot
Match: Q84W65 (SufE-like protein 1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SUFE1 PE=1 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 2.4e-13
Identity = 48/143 (33.57%), Postives = 76/143 (53.15%), Query Frame = 0

Query: 80  ELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESIRLPENRVKGCATQVWLDVK 139
           E +  K+  +V  F+S+ EP  + ++L+ Y   L P D   +  EN+V+GC +QVW+   
Sbjct: 81  EELPPKLQEIVKLFQSVQEPKAKYEQLMFYGKNLTPLDSQFKTRENKVEGCVSQVWVRAF 140

Query: 140 IDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVRSDDLDAVNVGL-HGKAISR 199
            DE   + ++ADSDS + KG  + L+  L G    E+L++  D   AV +GL    + SR
Sbjct: 141 FDEERNVVYEADSDSVLTKGLAALLVKGLSGRPVPEILRITPD--FAVLLGLQQSLSPSR 200

Query: 200 VNTWQNVLMSMQMRTSALVSRSE 222
            N   N+L  MQ +   L  + E
Sbjct: 201 NNGLLNMLKLMQKKALHLEVKGE 221

BLAST of MS000928 vs. ExPASy Swiss-Prot
Match: P74523 (Uncharacterized SufE-like protein slr1419 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1419 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.3e-11
Identity = 38/140 (27.14%), Postives = 78/140 (55.71%), Query Frame = 0

Query: 76  VYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESIRLPENRVKGCATQVW 135
           V A+  +   + ++V  F+   +P  R ++LL Y   L+P  E  ++  N+V+GC +QV+
Sbjct: 12  VMANATLPPNLAKIVERFQRHTDPKKRYEQLLWYGKKLEPMMEEGKIAANKVQGCVSQVY 71

Query: 136 LDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVRSDDLDAVNVGLHGKA 195
           +   +++  ++ ++ DSD+++ KG  + LI  L+G  P E++++  D ++A  + +    
Sbjct: 72  ITADLED-GKVMYQGDSDAQLVKGLVALLIQGLNGLTPTEIVELTPDFIEATGLQV-SLT 131

Query: 196 ISRVNTWQNVLMSMQMRTSA 216
            SR N + N+   MQ +  A
Sbjct: 132 PSRANGFYNIFKMMQTKAIA 149

BLAST of MS000928 vs. ExPASy TrEMBL
Match: A0A6J1BPL7 (sufE-like protein 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111004673 PE=4 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.0e-128
Identity = 233/236 (98.73%), Postives = 235/236 (99.58%), Query Frame = 0

Query: 1   MSSIPIITSCGFGFAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 60
           MSSIPIITSCGFGFAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL
Sbjct: 1   MSSIPIITSCGFGFAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 60

Query: 61  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 120
           CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI
Sbjct: 61  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 120

Query: 121 RLPENRVKGCATQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 180
           RLPENRVKGC TQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR
Sbjct: 121 RLPENRVKGCXTQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 180

Query: 181 SDDLDAVNVGLHGKAISRVNTWQNVLMSMQMRTSALVSRSEPKPPLEPFIITVHHR 237
           SDDLDAVNVGLHGKAISRVNTWQNV+MSMQMRT+ALVSRSEPKPPLEPFIITVHHR
Sbjct: 181 SDDLDAVNVGLHGKAISRVNTWQNVMMSMQMRTNALVSRSEPKPPLEPFIITVHHR 236

BLAST of MS000928 vs. ExPASy TrEMBL
Match: A0A5A7UYK2 (SufE-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001910 PE=4 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.3e-99
Identity = 187/237 (78.90%), Postives = 206/237 (86.92%), Query Frame = 0

Query: 1   MSSIPIITSCGFGFAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 60
           MSSIP+I S    F++FS SSKL F++ R   V N RF ASKSVRCAISHDLN+TSSNKL
Sbjct: 1   MSSIPMIASSRIAFSDFSGSSKLHFQQFRSPHVVNRRFAASKSVRCAISHDLNLTSSNKL 60

Query: 61  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 120
           CS RP CDLD+I IPVY+SELVADK+ RLVLEFESLLEPIDRVKRLLHYAAIL  SD++I
Sbjct: 61  CSPRPNCDLDSIAIPVYSSELVADKLRRLVLEFESLLEPIDRVKRLLHYAAILGSSDKAI 120

Query: 121 RLPENRVKGCATQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 180
           R+PENRV+GCA QVWLDVKIDEF+RMRFKADSDSEIAKGYCSCLIWML+GAEP EVL VR
Sbjct: 121 RIPENRVRGCAAQVWLDVKIDEFDRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLNVR 180

Query: 181 SDDLDAVNVGLHGKAISRVNTWQNVLMSMQMRTSALVSRSEPKPPLEPFII-TVHHR 237
           SDDL+AVNVGLHGKA SRVNTWQNVLMSMQMRT+ LVS++E KP LEPF   TVH R
Sbjct: 181 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSKTEVKPLLEPFTFRTVHRR 237

BLAST of MS000928 vs. ExPASy TrEMBL
Match: A0A1S3BAY5 (sufE-like protein 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487725 PE=4 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 3.6e-97
Identity = 185/237 (78.06%), Postives = 202/237 (85.23%), Query Frame = 0

Query: 1   MSSIPIITSCGFGFAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 60
           MSSIP+I S    F++FS SSKL F++ R   V N RF ASKSVRCAISHDLN+TSSNKL
Sbjct: 1   MSSIPMIASSRIAFSDFSGSSKLHFQQFRSPHVVNRRFAASKSVRCAISHDLNLTSSNKL 60

Query: 61  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 120
           CS RP CDLD+I IPVY+SELVADK+ RLVLEFESLLEPIDRVKRLLHYAAIL  SD++I
Sbjct: 61  CSPRPNCDLDSIAIPVYSSELVADKLRRLVLEFESLLEPIDRVKRLLHYAAILGSSDKAI 120

Query: 121 RLPENRVKGCATQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 180
           R+PENRV+GCA QVWLDVKIDEF+RMRFKADSDSEIAKGYCSCLIWML+GAEP EVL VR
Sbjct: 121 RIPENRVRGCAAQVWLDVKIDEFDRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLNVR 180

Query: 181 SDDLDAVNVGLHGKAISRVNTWQNVLMSMQMRTSALVSRSEPKPPLEPFII-TVHHR 237
           SDDL+AVNVGLHGKA SRVNTWQNVLMSMQMRT+ L      KPPLEPF   TVH R
Sbjct: 181 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTL------KPPLEPFTFRTVHRR 231

BLAST of MS000928 vs. ExPASy TrEMBL
Match: A0A0A0LMT7 (SufE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G349630 PE=4 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 1.2e-89
Identity = 177/237 (74.68%), Postives = 192/237 (81.01%), Query Frame = 0

Query: 2   SSIPIITSCGFG-FAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 61
           S IP+  S   G F +FS SS L F++ RF ++ N    AS      ISH LN+TSSNKL
Sbjct: 3   SIIPVTASSPLGFFPQFSASSNLHFQQFRFPRLLNRTLAAS------ISHHLNLTSSNKL 62

Query: 62  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 121
           CS RP CDL +I +PVY SE VADK+ RLVLEFESLLEPIDRVKRLLHYAAILD SDE+I
Sbjct: 63  CSPRPNCDLHSIALPVYTSESVADKLRRLVLEFESLLEPIDRVKRLLHYAAILDSSDEAI 122

Query: 122 RLPENRVKGCATQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 181
           RLPENRVKGCA QVWLDV +DEF RMRFKADSDSEIAKGYCSCLIWML+GAEP EVLKVR
Sbjct: 123 RLPENRVKGCAAQVWLDVNVDEFGRMRFKADSDSEIAKGYCSCLIWMLEGAEPWEVLKVR 182

Query: 182 SDDLDAVNVGLHGKAISRVNTWQNVLMSMQMRTSALVSRSEPKPPLEPFII-TVHHR 237
           SDDL+AVNVGLHGKA SRVNTWQNVLMSMQMRT+ LVS SE KPPLEPF   TVH R
Sbjct: 183 SDDLEAVNVGLHGKATSRVNTWQNVLMSMQMRTNTLVSNSEMKPPLEPFTSRTVHRR 233

BLAST of MS000928 vs. ExPASy TrEMBL
Match: A0A6J1KU63 (sufE-like protein 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111496439 PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.6e-84
Identity = 166/221 (75.11%), Postives = 188/221 (85.07%), Query Frame = 0

Query: 1   MSSIPIITSCGFGFAEFSDSSKLSFERIRFRQVENTRFLASKSVRCAISHDLNVTSSNKL 60
           M+S+PI+T  GF  AEFS SSKL  +++R R V N RFLASKS+R  IS      SS++ 
Sbjct: 1   MASMPIVT-FGFRSAEFSGSSKLHLQKMRSRYVVNQRFLASKSLRRTIS------SSDRF 60

Query: 61  CSRRPKCDLDAITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESI 120
             R   CDLDA  IPVY S+LV DK+HRLVLEF+ LL+PIDRVKRLL YAAILDPS ESI
Sbjct: 61  HLRHSNCDLDAFAIPVYTSDLVVDKLHRLVLEFKPLLKPIDRVKRLLQYAAILDPSGESI 120

Query: 121 RLPENRVKGCATQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVR 180
           RLPENRV+GCATQVWLDV+IDEF+RMRFKADSDSEIAKGYCSCLIWML+GA+P EVLK+R
Sbjct: 121 RLPENRVRGCATQVWLDVRIDEFDRMRFKADSDSEIAKGYCSCLIWMLEGAKPCEVLKMR 180

Query: 181 SDDLDAVNVGLHGKAISRVNTWQNVLMSMQMRTSALVSRSE 222
           SDDL+AVNVGLHGKAISRVNTWQNVLMSMQMRT+ALVSR+E
Sbjct: 181 SDDLEAVNVGLHGKAISRVNTWQNVLMSMQMRTNALVSRNE 214

BLAST of MS000928 vs. TAIR 10
Match: AT1G67810.1 (sulfur E2 )

HSP 1 Score: 204.9 bits (520), Expect = 7.1e-53
Identity = 111/208 (53.37%), Postives = 138/208 (66.35%), Query Frame = 0

Query: 14  FAEFSDSSKLSFERIRFRQVENTRF---LASKSVRCAISHDLNVTSSNKLCSRRPKCDLD 73
           F   +    +S  R   +   N RF    + K + C +   LN+ S+ K  S      L 
Sbjct: 7   FKALASPPLISTSRPTTKSFPNPRFTSRFSPKPITC-MRDSLNLGSNPKAPS---PFSLA 66

Query: 74  AITIPVYASELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESIRLPENRVKGC 133
            +++        +DK+  LV EF SL EPIDRVKRLL+YAA L P DES R+ ENRV GC
Sbjct: 67  TVSVDAPLGTKTSDKLRILVSEFRSLTEPIDRVKRLLNYAATLAPLDESARISENRVTGC 126

Query: 134 ATQVWLDVKIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVRSDDLDAVNVG 193
            TQVWL++K+DEF RMRFKADSDSEI+KG+CSCLIW+LDGA+P EV+ VRS+DL  +NVG
Sbjct: 127 TTQVWLEIKMDEFGRMRFKADSDSEISKGFCSCLIWILDGAKPEEVMGVRSEDLSEMNVG 186

Query: 194 LHGKAISRVNTWQNVLMSMQMRTSALVS 219
           +HGK  SRVNTW NVLMSMQ RT  LV+
Sbjct: 187 VHGKEQSRVNTWHNVLMSMQKRTMTLVA 210

BLAST of MS000928 vs. TAIR 10
Match: AT5G50210.1 (quinolinate synthase )

HSP 1 Score: 165.2 bits (417), Expect = 6.2e-41
Identity = 84/160 (52.50%), Postives = 114/160 (71.25%), Query Frame = 0

Query: 79  SELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESIRLPENRVKGCATQVWLDV 138
           +ELV  K+ RLV EF+SL EPIDR+K +LHYA++L    ES +   NRV GC  +VWLD 
Sbjct: 81  TELVPYKLQRLVKEFKSLTEPIDRLKWVLHYASLLPQMPESSKTESNRVMGCTARVWLDA 140

Query: 139 KIDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVRSDDLDAVNVGLHGKAISR 198
           ++ +  +MRF ADSDS+++KG CSCLI +LD A P+EV++++++DL  +NVGL G   SR
Sbjct: 141 ELGQDGKMRFCADSDSDVSKGMCSCLIQVLDEASPVEVMELKTEDLAELNVGLLGGERSR 200

Query: 199 VNTWQNVLMSMQMRTSALVSRSEPK-PPLEPF---IITVH 235
           VNTW NVL+SMQ +T  LV+  E K P  EPF   ++T H
Sbjct: 201 VNTWYNVLVSMQKKTRRLVAEREGKVPSFEPFPSLVLTAH 240

BLAST of MS000928 vs. TAIR 10
Match: AT4G26500.1 (chloroplast sulfur E )

HSP 1 Score: 77.4 bits (189), Expect = 1.7e-14
Identity = 48/143 (33.57%), Postives = 76/143 (53.15%), Query Frame = 0

Query: 80  ELVADKIHRLVLEFESLLEPIDRVKRLLHYAAILDPSDESIRLPENRVKGCATQVWLDVK 139
           E +  K+  +V  F+S+ EP  + ++L+ Y   L P D   +  EN+V+GC +QVW+   
Sbjct: 81  EELPPKLQEIVKLFQSVQEPKAKYEQLMFYGKNLTPLDSQFKTRENKVEGCVSQVWVRAF 140

Query: 140 IDEFERMRFKADSDSEIAKGYCSCLIWMLDGAEPLEVLKVRSDDLDAVNVGL-HGKAISR 199
            DE   + ++ADSDS + KG  + L+  L G    E+L++  D   AV +GL    + SR
Sbjct: 141 FDEERNVVYEADSDSVLTKGLAALLVKGLSGRPVPEILRITPD--FAVLLGLQQSLSPSR 200

Query: 200 VNTWQNVLMSMQMRTSALVSRSE 222
            N   N+L  MQ +   L  + E
Sbjct: 201 NNGLLNMLKLMQKKALHLEVKGE 221

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131476.12.2e-12898.73sufE-like protein 2, chloroplastic [Momordica charantia][more]
XP_038886328.15.7e-10581.86sufE-like protein 2, chloroplastic [Benincasa hispida][more]
KAA0061053.12.7e-9978.90sufE-like protein 2 [Cucumis melo var. makuwa][more]
XP_008444379.17.5e-9778.06PREDICTED: sufE-like protein 2, chloroplastic [Cucumis melo][more]
XP_004143058.12.6e-8974.68sufE-like protein 2, chloroplastic [Cucumis sativus] >KGN62329.1 hypothetical pr... [more]
Match NameE-valueIdentityDescription
Q9FXE39.9e-5253.37SufE-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SUFE2 PE=2... [more]
Q9FGS48.7e-4052.50Quinolinate synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=QS PE=1 S... [more]
Q2QTL08.5e-2746.58Quinolinate synthase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q84W652.4e-1333.57SufE-like protein 1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702... [more]
P745231.3e-1127.14Uncharacterized SufE-like protein slr1419 OS=Synechocystis sp. (strain PCC 6803 ... [more]
Match NameE-valueIdentityDescription
A0A6J1BPL71.0e-12898.73sufE-like protein 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11100467... [more]
A0A5A7UYK21.3e-9978.90SufE-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G... [more]
A0A1S3BAY53.6e-9778.06sufE-like protein 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487725 PE=4 ... [more]
A0A0A0LMT71.2e-8974.68SufE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G349630 PE=4 S... [more]
A0A6J1KU631.6e-8475.11sufE-like protein 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111496439 P... [more]
Match NameE-valueIdentityDescription
AT1G67810.17.1e-5353.37sulfur E2 [more]
AT5G50210.16.2e-4152.50quinolinate synthase [more]
AT4G26500.11.7e-1433.57chloroplast sulfur E [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003808Fe-S metabolism associated domain, SufE-likePFAMPF02657SufEcoord: 92..211
e-value: 2.4E-24
score: 85.5
IPR003808Fe-S metabolism associated domain, SufE-likePANTHERPTHR43597SULFUR ACCEPTOR PROTEIN CSDEcoord: 42..231
NoneNo IPR availableGENE3D3.90.1010.10coord: 81..218
e-value: 2.4E-36
score: 126.3
NoneNo IPR availablePANTHERPTHR43597:SF5SUFE-LIKE PROTEIN 2, CHLOROPLASTICcoord: 42..231
NoneNo IPR availableSUPERFAMILY82649SufE/NifUcoord: 84..217

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000928.1MS000928.1mRNA