MS000820 (gene) Bitter gourd (TR) v1

Overview


Name	MS000820
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNase H domain-containing protein
Location	scaffold236: 94140 .. 94547 (+)
RNA-Seq Expression	MS000820
Synteny	MS000820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGAGACCTCCCTCAAGTAACCATTGGAGGCTTAATACTGATGCGGCATGGCATGCAGAAAATGGAACAGAAGGGCTCGGGTGGGTCTATCGAGATGAAAGAGGGAAAGTGATTAGGGCTGGGTGCACACCAATTTTCAGAGACTATGGAGTTACTTACTTGGAGCTAATGGCAATCTGTGATGGGCTAGACGCATTGGTGAACAAAGGCACTGAACCGATTCATCTAGAGACGGATTCCATGAAGGATGTTCAATTTCTTCAGGGCAAAATGGATGTCTTCACAGAAATTTCTTGGCTTCTGGAGGATATCAGAAATTATGAAGGAGGAAGGAGAATTGGATCAATCCAACATGTTAAGAGGACCTCAAATTCGGTAGCTCATGAGATTGCTCGATTTTCGATGGAA

mRNA sequence

TGGAGACCTCCCTCAAGTAACCATTGGAGGCTTAATACTGATGCGGCATGGCATGCAGAAAATGGAACAGAAGGGCTCGGGTGGGTCTATCGAGATGAAAGAGGGAAAGTGATTAGGGCTGGGTGCACACCAATTTTCAGAGACTATGGAGTTACTTACTTGGAGCTAATGGCAATCTGTGATGGGCTAGACGCATTGGTGAACAAAGGCACTGAACCGATTCATCTAGAGACGGATTCCATGAAGGATGTTCAATTTCTTCAGGGCAAAATGGATGTCTTCACAGAAATTTCTTGGCTTCTGGAGGATATCAGAAATTATGAAGGAGGAAGGAGAATTGGATCAATCCAACATGTTAAGAGGACCTCAAATTCGGTAGCTCATGAGATTGCTCGATTTTCGATGGAA

Coding sequence (CDS)

TGGAGACCTCCCTCAAGTAACCATTGGAGGCTTAATACTGATGCGGCATGGCATGCAGAAAATGGAACAGAAGGGCTCGGGTGGGTCTATCGAGATGAAAGAGGGAAAGTGATTAGGGCTGGGTGCACACCAATTTTCAGAGACTATGGAGTTACTTACTTGGAGCTAATGGCAATCTGTGATGGGCTAGACGCATTGGTGAACAAAGGCACTGAACCGATTCATCTAGAGACGGATTCCATGAAGGATGTTCAATTTCTTCAGGGCAAAATGGATGTCTTCACAGAAATTTCTTGGCTTCTGGAGGATATCAGAAATTATGAAGGAGGAAGGAGAATTGGATCAATCCAACATGTTAAGAGGACCTCAAATTCGGTAGCTCATGAGATTGCTCGATTTTCGATGGAA

Protein sequence

WRPPSSNHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAICDGLDALVNKGTEPIHLETDSMKDVQFLQGKMDVFTEISWLLEDIRNYEGGRRIGSIQHVKRTSNSVAHEIARFSME

Homology

[Close]

BLAST of MS000820 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 144.4 bits (363), Expect = 7.0e-31
Identity = 65/136 (47.79%), Postives = 93/136 (68.38%), Query Frame = 0


Query: 1   WRPPSSNHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAIC 60
           W+PP+SN W+LNT+AAW A+  T G+GW+ RDE+G+VI+A C  I  +  +TYLE+MAIC
Sbjct: 79  WKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAIC 138

Query: 61  DGLDALVNKGTEPIHLETDSMKDVQFLQGKMDVFTEISWLLEDIRNYEGGRRIGSIQHVK 120
           +GL A+  +   PIHLE+DS++ +  L  +    TEI WLLE+I        I S++H+ 
Sbjct: 139 EGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHIS 198

Query: 121 RTSNSVAHEIARFSME 137
           R +N VAH +AR +ME
Sbjct: 199 REANKVAHGLARRAME 214

[Close]

BLAST of MS000820 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 141.4 bits (355), Expect = 5.9e-30
Identity = 66/144 (45.83%), Postives = 95/144 (65.97%), Query Frame = 0


Query: 1   WRPPSSNHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAIC 60
           W+PP+SN W+LNTDAAW A+  T G+GW+ RDE+G+VI+A C  I  +  +TYLE+MAIC
Sbjct: 79  WKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAIC 138

Query: 61  DGLDA--------LVNKGTEPIHLETDSMKDVQFLQGKMDVFTEISWLLEDIRNYEGGRR 120
           +GL A        +  +   PIHLE+DS++ +  L  +    TEI WLLE+I       +
Sbjct: 139 EGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMK 198

Query: 121 IGSIQHVKRTSNSVAHEIARFSME 137
           I S++H+ R +N VAH++AR +ME
Sbjct: 199 IVSMRHISREANKVAHDLARRAME 222

[Close]

BLAST of MS000820 vs. NCBI nr
Match: XP_022148737.1 (uncharacterized protein LOC111017329 [Momordica charantia])

HSP 1 Score: 121.3 bits (303), Expect = 6.4e-24
Identity = 56/125 (44.80%), Postives = 83/125 (66.40%), Query Frame = 0


Query: 7   NHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAICDGLDAL 66
           N W+LNTDAAWHA  G  GLGW+ R+E+ ++ +AG   I     +TYLELMAI  G++A+
Sbjct: 11  NFWKLNTDAAWHANQGVGGLGWIIRNEKREIKKAGGQTITDARDITYLELMAIWLGIEAV 70

Query: 67  VNKGTEPIHLETDSMKDVQFLQGKMDVFTEISWLLEDIRNYEGGRRIGSIQHVKRTSNSV 126
           V+  +  + +E++S++ +  +QG     TEI WL++DI+N+    R+   QHV+R  NSV
Sbjct: 71  VSSSSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSV 130

Query: 127 AHEIA 132
           A EIA
Sbjct: 131 AQEIA 135

[Close]

BLAST of MS000820 vs. NCBI nr
Match: XP_022154990.1 (uncharacterized protein LOC111022134 isoform X1 [Momordica charantia])

HSP 1 Score: 115.2 bits (287), Expect = 4.6e-22
Identity = 46/87 (52.87%), Postives = 66/87 (75.86%), Query Frame = 0


Query: 1   WRPPSSNHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAIC 60
           W+PP+SN W+LNTDAAW A+  T+G+GW+ RDE+G+VI+ GC  I  +  +TYLE+MAIC
Sbjct: 255 WKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAIC 314

Query: 61  DGLDALVNKGTEPIHLETDSMKDVQFL 88
           +GL A+  +   PIHLE+DS++ +  L
Sbjct: 315 EGLRAIRQEHCRPIHLESDSLEAIHLL 341

[Close]

BLAST of MS000820 vs. NCBI nr
Match: XP_022154991.1 (uncharacterized protein LOC111022134 isoform X2 [Momordica charantia])

HSP 1 Score: 115.2 bits (287), Expect = 4.6e-22
Identity = 46/87 (52.87%), Postives = 66/87 (75.86%), Query Frame = 0


Query: 1   WRPPSSNHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAIC 60
           W+PP+SN W+LNTDAAW A+  T+G+GW+ RDE+G+VI+ GC  I  +  +TYLE+MAIC
Sbjct: 220 WKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAIC 279

Query: 61  DGLDALVNKGTEPIHLETDSMKDVQFL 88
           +GL A+  +   PIHLE+DS++ +  L
Sbjct: 280 EGLRAIRQEHCRPIHLESDSLEAIHLL 306

[Close]

BLAST of MS000820 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 3.4e-31
Identity = 65/136 (47.79%), Postives = 93/136 (68.38%), Query Frame = 0


Query: 1   WRPPSSNHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAIC 60
           W+PP+SN W+LNT+AAW A+  T G+GW+ RDE+G+VI+A C  I  +  +TYLE+MAIC
Sbjct: 79  WKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAIC 138

Query: 61  DGLDALVNKGTEPIHLETDSMKDVQFLQGKMDVFTEISWLLEDIRNYEGGRRIGSIQHVK 120
           +GL A+  +   PIHLE+DS++ +  L  +    TEI WLLE+I        I S++H+ 
Sbjct: 139 EGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHIS 198

Query: 121 RTSNSVAHEIARFSME 137
           R +N VAH +AR +ME
Sbjct: 199 REANKVAHGLARRAME 214

[Close]

BLAST of MS000820 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.9e-30
Identity = 66/144 (45.83%), Postives = 95/144 (65.97%), Query Frame = 0


Query: 1   WRPPSSNHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAIC 60
           W+PP+SN W+LNTDAAW A+  T G+GW+ RDE+G+VI+A C  I  +  +TYLE+MAIC
Sbjct: 79  WKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAIC 138

Query: 61  DGLDA--------LVNKGTEPIHLETDSMKDVQFLQGKMDVFTEISWLLEDIRNYEGGRR 120
           +GL A        +  +   PIHLE+DS++ +  L  +    TEI WLLE+I       +
Sbjct: 139 EGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMK 198

Query: 121 IGSIQHVKRTSNSVAHEIARFSME 137
           I S++H+ R +N VAH++AR +ME
Sbjct: 199 IVSMRHISREANKVAHDLARRAME 222

[Close]

BLAST of MS000820 vs. ExPASy TrEMBL
Match: A0A6J1D5W1 (uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017329 PE=4 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 3.1e-24
Identity = 56/125 (44.80%), Postives = 83/125 (66.40%), Query Frame = 0


Query: 7   NHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAICDGLDAL 66
           N W+LNTDAAWHA  G  GLGW+ R+E+ ++ +AG   I     +TYLELMAI  G++A+
Sbjct: 11  NFWKLNTDAAWHANQGVGGLGWIIRNEKREIKKAGGQTITDARDITYLELMAIWLGIEAV 70

Query: 67  VNKGTEPIHLETDSMKDVQFLQGKMDVFTEISWLLEDIRNYEGGRRIGSIQHVKRTSNSV 126
           V+  +  + +E++S++ +  +QG     TEI WL++DI+N+    R+   QHV+R  NSV
Sbjct: 71  VSSSSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSV 130

Query: 127 AHEIA 132
           A EIA
Sbjct: 131 AQEIA 135

[Close]

BLAST of MS000820 vs. ExPASy TrEMBL
Match: A0A6J1DL64 (uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.2e-22
Identity = 46/87 (52.87%), Postives = 66/87 (75.86%), Query Frame = 0


Query: 1   WRPPSSNHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAIC 60
           W+PP+SN W+LNTDAAW A+  T+G+GW+ RDE+G+VI+ GC  I  +  +TYLE+MAIC
Sbjct: 255 WKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAIC 314

Query: 61  DGLDALVNKGTEPIHLETDSMKDVQFL 88
           +GL A+  +   PIHLE+DS++ +  L
Sbjct: 315 EGLRAIRQEHCRPIHLESDSLEAIHLL 341

[Close]

BLAST of MS000820 vs. ExPASy TrEMBL
Match: A0A6J1DQC9 (uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.2e-22
Identity = 46/87 (52.87%), Postives = 66/87 (75.86%), Query Frame = 0


Query: 1   WRPPSSNHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAIC 60
           W+PP+SN W+LNTDAAW A+  T+G+GW+ RDE+G+VI+ GC  I  +  +TYLE+MAIC
Sbjct: 220 WKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAIC 279

Query: 61  DGLDALVNKGTEPIHLETDSMKDVQFL 88
           +GL A+  +   PIHLE+DS++ +  L
Sbjct: 280 EGLRAIRQEHCRPIHLESDSLEAIHLL 306

[Close]

BLAST of MS000820 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 8.0e-09
Identity = 40/135 (29.63%), Postives = 58/135 (42.96%), Query Frame = 0


Query: 1   WRPPSSNHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAIC 60
           W+ P     + NTDA W  EN   G+GW+ R+E G V+  G   + R   V   EL A+ 
Sbjct: 137 WKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALR 196

Query: 61  DGLDALVNKGTEPIHLETDSMKDVQFLQGKMDVFTEISWLLEDIRNYEGGRRIGSIQHVK 120
             +  +     + I  E+D+   V  L    D +  +   LEDI+           +   
Sbjct: 197 WAVLTMSRFNYKRIIFESDAQALVNLLNSD-DFWPTLQPALEDIQQLLHHFEEVKFEFTP 256

Query: 121 RTSNSVAHEIARFSM 136
           R  N VA  IAR S+
Sbjct: 257 RGGNKVADRIARESI 270

[Close]

BLAST of MS000820 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 8.0e-09
Identity = 35/135 (25.93%), Postives = 64/135 (47.41%), Query Frame = 0


Query: 1   WRPPSSNHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAIC 60
           WRPP     + NTDA W+ +N   G+GWV R+E+G+V   G   + +   V   EL A+ 
Sbjct: 420 WRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMR 479

Query: 61  DGLDALVNKGTEPIHLETDSMKDVQFLQGKMDVFTEISWLLEDIRNYEGGRRIGSIQHVK 120
             + +L       +  E+DS   ++ L    +++  +   ++D++             + 
Sbjct: 480 WAVLSLSRFQYNYVIFESDSQVLIEILNND-EIWPSLKPTIQDLQRLLSQFTEVKFVFIP 539

Query: 121 RTSNSVAHEIARFSM 136
           R  N++A  +AR S+
Sbjct: 540 REGNTLAERVARESL 553

[Close]

BLAST of MS000820 vs. TAIR 10
Match: AT5G61090.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 42.4 bits (98), Expect = 3.5e-04
Identity = 36/136 (26.47%), Postives = 55/136 (40.44%), Query Frame = 0


Query: 1   WRPPSSNHWRLNTDAAWHAENGTEGLGWVYRDERGKVIRAGCTPIFRDYGVTYLELMAIC 60
           W PP     +LN     +  +       + RD+ GK +  G     +        L+AI 
Sbjct: 499 WTPPLV---KLNIQGTSNPLSDLTRSAGIVRDQSGKWV-FGYIRCHKSIPEVVAGLLAIY 558

Query: 61  DGLDALVNKGTEPIHLETDSMKDVQFLQGKMDVFTEISWLLEDIRNYEGGRRIGSIQHVK 120
            GL  L + G   IHLET S + +  L  K  +F +   LL   ++         I H+ 
Sbjct: 559 QGLKYLWDSGFRRIHLETTSFEIINALTTKSSLFCKSKTLLGACKDMILKEWECDIYHIS 618

Query: 121 RTSNSVAHEIARFSME 137
           +  NS A  +A+ S E
Sbjct: 619 KEQNSCAEWLAKRSEE 630

The following BLAST results are available for this feature:

BLAST of MS000820 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143535.1	7.0e-31	47.79	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022156777.1	5.9e-30	45.83	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_022148737.1	6.4e-24	44.80	uncharacterized protein LOC111017329 [Momordica charantia]	[more]
XP_022154990.1	4.6e-22	52.87	uncharacterized protein LOC111022134 isoform X1 [Momordica charantia]	[more]
XP_022154991.1	4.6e-22	52.87	uncharacterized protein LOC111022134 isoform X2 [Momordica charantia]	[more]

BLAST of MS000820 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS000820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CP26	3.4e-31	47.79	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DSV1	2.9e-30	45.83	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1D5W1	3.1e-24	44.80	uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A6J1DL64	2.2e-22	52.87	uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DQC9	2.2e-22	52.87	uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of MS000820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34320.1	8.0e-09	29.63	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G29090.1	8.0e-09	25.93	Ribonuclease H-like superfamily protein	[more]
AT5G61090.1	3.5e-04	26.47	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 12..133 e-value: 1.2E-18 score: 67.1
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 8..136 e-value: 7.9E-14 score: 53.8
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 1..134
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 11..132 e-value: 2.75463E-19 score: 75.4284
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 7..132

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000820.1	MS000820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity