MS000459 (gene) Bitter gourd (TR) v1

Overview


Name	MS000459
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	ethylene-responsive transcription factor CRF4-like
Location	scaffold44: 1853051 .. 1854043 (+)
RNA-Seq Expression	MS000459
Synteny	MS000459

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGATACTCTGTTTTGTCCAGTTAAGTTCACTGAGCACCGAAACTTCACCAAGAAATTTTCCACCAAGAAGCAGCAGCAGAATCAGCATCACCCGCCGGAAAATAGAGTTGTACGGATATCGGTAACTGACCCGGACGCGACGGATTCGTCCAGTGACGAAGAGGAGGGGGAGTTTTTTGAGCGGCAGAGAATTAAGAAATACGTGAACGAGATCAAAATCGAAAGCGGGTCCAGAAATAGCCTTGTACCCGGTTGCCGGAAGCGGCCGGCCGGTGAGGGTTCCGAGTTCCGGCGCCCGGTGAAGCCGCCTCCGACAACCAATGGGAAGAAATTTCGCGGCGTGCGGCAGAGGCCTTGGGGGAAATGGGCGGCGGAGATTAGAGATCCTGCTCGCCGTCAGCGGGTGTGGCTGGGGACTTTCGACACGGCGGAGGAAGCCGCCATGGTTTACGACAACGCCGCCATTAAGCTCCGTGGGCCGGACGCCCTCACCAATTTCGCAACCCCGCCACCGCCGCCGGAGAAGGAAGCGGAGGTTAATATCCCCTCTGTTTCCGGGTCCTACTACGACTCCGGCGAGGAGTCTCACAATTTGTCATCCCCAACTTCGGTTCTCCACTTCAGAACTCAGTCGCCGGAAGAATCCGAAAACCCCCCAAAACCCGAAGCGTTTCAGAAACCGTCGCCGCCGTCGCTGGCCGACGACCAATTTCACGAATGTCAAGGCGAAACCTCTTTCTCCGGAGAATATTCCACCGATTTCCCCAAGTTCGAGGACATATTCCAACTTCCGAGCCCCGACATGTCGATTTTCTTCGACGAACAGCCGCTGTTCTTCGAGGAGAGCATTTGGAACGAAGGTTTCAGCCAGATTTTCACCAACATGCCGGAAGATTTGGGATCGCCGGTGCTATCTTCTTCGATTAGCCAAGGGGGCGACGATTATTTCCAAGATATTTTATTGGGGTCCGACCCTTTGGTGGTTCTC

mRNA sequence

ATGGAGGATACTCTGTTTTGTCCAGTTAAGTTCACTGAGCACCGAAACTTCACCAAGAAATTTTCCACCAAGAAGCAGCAGCAGAATCAGCATCACCCGCCGGAAAATAGAGTTGTACGGATATCGGTAACTGACCCGGACGCGACGGATTCGTCCAGTGACGAAGAGGAGGGGGAGTTTTTTGAGCGGCAGAGAATTAAGAAATACGTGAACGAGATCAAAATCGAAAGCGGGTCCAGAAATAGCCTTGTACCCGGTTGCCGGAAGCGGCCGGCCGGTGAGGGTTCCGAGTTCCGGCGCCCGGTGAAGCCGCCTCCGACAACCAATGGGAAGAAATTTCGCGGCGTGCGGCAGAGGCCTTGGGGGAAATGGGCGGCGGAGATTAGAGATCCTGCTCGCCGTCAGCGGGTGTGGCTGGGGACTTTCGACACGGCGGAGGAAGCCGCCATGGTTTACGACAACGCCGCCATTAAGCTCCGTGGGCCGGACGCCCTCACCAATTTCGCAACCCCGCCACCGCCGCCGGAGAAGGAAGCGGAGGTTAATATCCCCTCTGTTTCCGGGTCCTACTACGACTCCGGCGAGGAGTCTCACAATTTGTCATCCCCAACTTCGGTTCTCCACTTCAGAACTCAGTCGCCGGAAGAATCCGAAAACCCCCCAAAACCCGAAGCGTTTCAGAAACCGTCGCCGCCGTCGCTGGCCGACGACCAATTTCACGAATGTCAAGGCGAAACCTCTTTCTCCGGAGAATATTCCACCGATTTCCCCAAGTTCGAGGACATATTCCAACTTCCGAGCCCCGACATGTCGATTTTCTTCGACGAACAGCCGCTGTTCTTCGAGGAGAGCATTTGGAACGAAGGTTTCAGCCAGATTTTCACCAACATGCCGGAAGATTTGGGATCGCCGGTGCTATCTTCTTCGATTAGCCAAGGGGGCGACGATTATTTCCAAGATATTTTATTGGGGTCCGACCCTTTGGTGGTTCTC

Coding sequence (CDS)

ATGGAGGATACTCTGTTTTGTCCAGTTAAGTTCACTGAGCACCGAAACTTCACCAAGAAATTTTCCACCAAGAAGCAGCAGCAGAATCAGCATCACCCGCCGGAAAATAGAGTTGTACGGATATCGGTAACTGACCCGGACGCGACGGATTCGTCCAGTGACGAAGAGGAGGGGGAGTTTTTTGAGCGGCAGAGAATTAAGAAATACGTGAACGAGATCAAAATCGAAAGCGGGTCCAGAAATAGCCTTGTACCCGGTTGCCGGAAGCGGCCGGCCGGTGAGGGTTCCGAGTTCCGGCGCCCGGTGAAGCCGCCTCCGACAACCAATGGGAAGAAATTTCGCGGCGTGCGGCAGAGGCCTTGGGGGAAATGGGCGGCGGAGATTAGAGATCCTGCTCGCCGTCAGCGGGTGTGGCTGGGGACTTTCGACACGGCGGAGGAAGCCGCCATGGTTTACGACAACGCCGCCATTAAGCTCCGTGGGCCGGACGCCCTCACCAATTTCGCAACCCCGCCACCGCCGCCGGAGAAGGAAGCGGAGGTTAATATCCCCTCTGTTTCCGGGTCCTACTACGACTCCGGCGAGGAGTCTCACAATTTGTCATCCCCAACTTCGGTTCTCCACTTCAGAACTCAGTCGCCGGAAGAATCCGAAAACCCCCCAAAACCCGAAGCGTTTCAGAAACCGTCGCCGCCGTCGCTGGCCGACGACCAATTTCACGAATGTCAAGGCGAAACCTCTTTCTCCGGAGAATATTCCACCGATTTCCCCAAGTTCGAGGACATATTCCAACTTCCGAGCCCCGACATGTCGATTTTCTTCGACGAACAGCCGCTGTTCTTCGAGGAGAGCATTTGGAACGAAGGTTTCAGCCAGATTTTCACCAACATGCCGGAAGATTTGGGATCGCCGGTGCTATCTTCTTCGATTAGCCAAGGGGGCGACGATTATTTCCAAGATATTTTATTGGGGTCCGACCCTTTGGTGGTTCTC

Protein sequence

MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEFFERQRIKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRPWGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPPPEKEAEVNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADDQFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNMPEDLGSPVLSSSISQGGDDYFQDILLGSDPLVVL

Homology

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BLAST of MS000459 vs. NCBI nr
Match: XP_022141160.1 (ethylene-responsive transcription factor CRF4-like [Momordica charantia])

HSP 1 Score: 668.7 bits (1724), Expect = 2.6e-188
Identity = 330/331 (99.70%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60
           MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF
Sbjct: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60

Query: 61  FERQRIKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120
           FERQR+KKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP
Sbjct: 61  FERQRVKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120

Query: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPPPEKEAE 180
           WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPPPEKEAE
Sbjct: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPPPEKEAE 180

Query: 181 VNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADDQFH 240
           VNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADDQFH
Sbjct: 181 VNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADDQFH 240

Query: 241 ECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNMPED 300
           ECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNMPED
Sbjct: 241 ECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNMPED 300

Query: 301 LGSPVLSSSISQGGDDYFQDILLGSDPLVVL 332
           LGSPVLSSSISQGGDDYFQDILLGSDPLVVL
Sbjct: 301 LGSPVLSSSISQGGDDYFQDILLGSDPLVVL 331

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BLAST of MS000459 vs. NCBI nr
Match: XP_004149454.1 (ethylene-responsive transcription factor CRF4 [Cucumis sativus] >KGN57872.1 hypothetical protein Csa_011137 [Cucumis sativus])

HSP 1 Score: 505.8 bits (1301), Expect = 2.9e-139
Identity = 258/334 (77.25%), Postives = 284/334 (85.03%), Query Frame = 0


Query: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60
           MEDTLF PVKFTEHRNFT KFS KK     HH  E+RVVRISVTDPDATDSSSDE+  +F
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKK-----HHQSESRVVRISVTDPDATDSSSDED--DF 60

Query: 61  FERQRIKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120
           FERQR+KKY+NEIKI+SG RN+L+P CRKRPAG+ SEFRR  K  P TNGKKFRGVRQRP
Sbjct: 61  FERQRVKKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRP 120

Query: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPPPEKEAE 180
           WGKWAAEIRDPARR R+WLGT+DTAEEAAMVYDNAAI+LRGPDALTNFATPPP P  E E
Sbjct: 121 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKE 180

Query: 181 V---NIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADD 240
           V   NIPSVSGSYYDS EESHNLSSPTSVLHFRT SPEESE PPK +  QKP P    DD
Sbjct: 181 VETANIPSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKP-PAPFVDD 240

Query: 241 QFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNM 300
           QFHECQGETSF+ EY T+FP+F+  F+ PSP+  IF D+QPLFFE+SIWN+ FS+IFTN+
Sbjct: 241 QFHECQGETSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNL 300

Query: 301 PEDLGSPVLSSSISQGGDDYFQDILLGSDPLVVL 332
           PED GSP+LSSSI QGGDDYFQDIL+GSDPLVVL
Sbjct: 301 PEDFGSPLLSSSIGQGGDDYFQDILMGSDPLVVL 326

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BLAST of MS000459 vs. NCBI nr
Match: XP_008439327.1 (PREDICTED: ethylene-responsive transcription factor CRF4-like [Cucumis melo] >KAA0033741.1 ethylene-responsive transcription factor CRF4-like [Cucumis melo var. makuwa] >TYJ99880.1 ethylene-responsive transcription factor CRF4-like [Cucumis melo var. makuwa])

HSP 1 Score: 500.7 bits (1288), Expect = 9.4e-138
Identity = 260/335 (77.61%), Postives = 284/335 (84.78%), Query Frame = 0


Query: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60
           MEDTLF PVKFTEHRNFT KFS KK     HH  E+RVVRISVTDPDATDSSSDE+  +F
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKK-----HHQSESRVVRISVTDPDATDSSSDED--DF 60

Query: 61  FERQRIKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120
           FERQR+KKY+NEIKI+SG RN+L+P CRKRPAG+ SEFRR  K  P TNGKKFRGVRQRP
Sbjct: 61  FERQRVKKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRP 120

Query: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPP--PPPEKE 180
           WGKWAAEIRDPARR R+WLGT+DTAEEAAMVYDNAAI+LRGPDALTNFATPP  P PEKE
Sbjct: 121 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPAVPMPEKE 180

Query: 181 AE-VNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADD 240
            E  NIPSVSGSYYDS EESHNLSSPTSVLHFRT SPEESE PPK +  QKP P    DD
Sbjct: 181 VETTNIPSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKP-PAPFVDD 240

Query: 241 QFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNM 300
           QFHECQGETSFS EY T+FP+F+  F+ PSP+  IF D+QPLFFE+SIWN+ FS+IFTN+
Sbjct: 241 QFHECQGETSFSEEYHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNDDFSEIFTNL 300

Query: 301 PEDLGSPVL-SSSISQGGDDYFQDILLGSDPLVVL 332
           PED GSP+L SSSI QGGDDYFQDIL GSDPLVVL
Sbjct: 301 PEDFGSPLLSSSSIGQGGDDYFQDILTGSDPLVVL 327

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BLAST of MS000459 vs. NCBI nr
Match: XP_038900136.1 (ethylene-responsive transcription factor CRF4-like [Benincasa hispida])

HSP 1 Score: 500.0 bits (1286), Expect = 1.6e-137
Identity = 257/334 (76.95%), Postives = 285/334 (85.33%), Query Frame = 0


Query: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60
           MEDTLF PVKFTEHRNFT KFS KK     HH  E+RVVRISVTDPDATDSSSDE+  ++
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKK-----HHHSESRVVRISVTDPDATDSSSDED--DY 60

Query: 61  FERQRIKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120
           FERQR+KKY+NEIKI+SG RN+L+P CRKRPAGE S+F+R  K  P  NGKKFRGVRQRP
Sbjct: 61  FERQRVKKYINEIKIQSGCRNNLLPSCRKRPAGERSDFQRQGKVAPPMNGKKFRGVRQRP 120

Query: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPP--PPEKE 180
           WGKWAAEIRDPARR R+WLGT+DTAEEAAMVYDNAAI+LRGPDALTNFATPPP   PEKE
Sbjct: 121 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVSMPEKE 180

Query: 181 AEV-NIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADD 240
            E+ NIPSVSGSYYDS EESHNLSSPTSVLHFRT SPEESE PPK +  Q P P    DD
Sbjct: 181 VEMANIPSVSGSYYDSCEESHNLSSPTSVLHFRTPSPEESEKPPKSDEVQNP-PAPFTDD 240

Query: 241 QFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNM 300
           QFHECQGETSFS E+ T+FP+F+  F+ PSP+  IF D+QPLFFE+SIWNE FS+IFTN+
Sbjct: 241 QFHECQGETSFSEEFHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNEDFSEIFTNL 300

Query: 301 PEDLGSPVLSSSISQGGDDYFQDILLGSDPLVVL 332
           PED GSP+LSSSISQGGDDYFQDIL+GSDPLVVL
Sbjct: 301 PEDFGSPLLSSSISQGGDDYFQDILMGSDPLVVL 326

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BLAST of MS000459 vs. NCBI nr
Match: KAG6576679.1 (Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014728.1 Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 495.4 bits (1274), Expect = 3.9e-136
Identity = 257/334 (76.95%), Postives = 282/334 (84.43%), Query Frame = 0


Query: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60
           MEDTLFCPVK TEHRNFT KFS KK     HH  E+RVVRISVTDPDATDSSSDEE  E 
Sbjct: 1   MEDTLFCPVKVTEHRNFTSKFSLKK-----HHQSESRVVRISVTDPDATDSSSDEE--EL 60

Query: 61  FERQRIKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120
           FERQR+KKY+NEIKI+SG RNSL+P CRKR AGE S+ RR  K  P TNGKKFRGVRQRP
Sbjct: 61  FERQRVKKYINEIKIQSGCRNSLLPSCRKRSAGEQSDSRRSGKVVPATNGKKFRGVRQRP 120

Query: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPP--PPPEKE 180
           WGKWAAEIRDPARR R+WLGT+DTAEEAAMVYDNAAI+LRGPDALTNFATP   P PEKE
Sbjct: 121 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPSSVPMPEKE 180

Query: 181 AEV-NIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADD 240
            EV NI SVSGSYYDS EESHNLSSPTSVLHFRT SPEE+E P KP+  Q+P PP  ADD
Sbjct: 181 VEVANIASVSGSYYDSSEESHNLSSPTSVLHFRTPSPEETEKPQKPDESQQP-PPPFADD 240

Query: 241 QFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNM 300
           QFHECQ +T+ S EY T+FP+F+D+F+LP P+  IF DEQPLFFEESIWNE FS+ FTN+
Sbjct: 241 QFHECQVKTTLSEEYYTEFPRFDDVFKLPIPEPPIFVDEQPLFFEESIWNEDFSEFFTNL 300

Query: 301 PEDLGSPVLSSSISQGGDDYFQDILLGSDPLVVL 332
           PED G+P+LSS ISQGGDDYFQDIL+GSDPLVVL
Sbjct: 301 PEDFGTPLLSSPISQGGDDYFQDILMGSDPLVVL 326

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BLAST of MS000459 vs. ExPASy Swiss-Prot
Match: Q9SUE3 (Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN=CRF4 PE=1 SV=2)

HSP 1 Score: 194.9 bits (494), Expect = 1.4e-48
Identity = 134/332 (40.36%), Postives = 199/332 (59.94%), Query Frame = 0


Query: 8   PVKFTEHRNFTKKFSTKKQQQNQHHPPEN-RVVRISVTDPDATDSSSDEEEGEFFERQRI 67
           PVK+TEH+   +K++ K   + +    ++ R+VR+S+TD DATDSSSDEEE   F R+R+
Sbjct: 11  PVKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATDSSSDEEE-FLFPRRRV 70

Query: 68  KKYVNEIKIESGSRN----SLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRPWG 127
           K+ +NEI++E  S +    S  P   ++     S  ++P       N KK+RGVRQRPWG
Sbjct: 71  KRLINEIRVEPSSSSTGDVSASPTKDRKRINVDSTVQKP--SVSGQNQKKYRGVRQRPWG 130

Query: 128 KWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPPPEKEAEVN 187
           KWAAEIRDP +R+R+WLGTF TAEEAA+VYDNAAIKLRGPDALTNF   P P   + +  
Sbjct: 131 KWAAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPVQEQEQ 190

Query: 188 IP--SVSGSYYDSGEESHNLSSPTSVLHFRT---QSPEESENPPKPEAFQKPSPPSLADD 247
            P  ++S S  +S ++S +LSSPTSVL+++T   + P +S   P  + F +P    ++  
Sbjct: 191 EPESNMSVSISESMDDSQHLSSPTSVLNYQTYVSEEPIDSLIKPVKQEFLEPEQEPIS-- 250

Query: 248 QFHECQGETSFSGE-YSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIF-- 307
            +H  +G T+ + + +  D    ++ F    PD+SIF  +QP+   +   N+ F+ +   
Sbjct: 251 -WHLGEGNTNTNDDSFPLDITFLDNYFNESLPDISIF--DQPMSPIQPTENDFFNDLMLF 310

Query: 308 -TNMPEDLGSPV--LSSSISQGGDDYFQDILL 324
            +N  E   S +  + SS +   D    D+LL
Sbjct: 311 DSNAEEYYSSEIKEIGSSFNDLDDSLISDLLL 334

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BLAST of MS000459 vs. ExPASy Swiss-Prot
Match: Q9FK12 (Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN=CRF3 PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 2.5e-48
Identity = 147/364 (40.38%), Postives = 201/364 (55.22%), Query Frame = 0


Query: 6   FCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF-FERQ 65
           F P+K+TEH+    K+ TKK  +        + V I  TDPDATDSSSDE+E +F F R+
Sbjct: 7   FRPLKYTEHKTSMTKY-TKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDFLFPRR 66

Query: 66  RIKKYVNEIKIESGSRNSLVPGC----RKRPAGEGSEFRRPVKPPPTTNG--------KK 125
           R+K++VNEI +E  S N++V G     RKR +    E + P       N         KK
Sbjct: 67  RVKRFVNEITVEP-SCNNVVTGVSMKDRKRLSSSSDETQSPASSRQRPNNKVSVSGQIKK 126

Query: 126 FRGVRQRPWGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPP 185
           FRGVRQRPWGKWAAEIRDP +R+R+WLGTF+TAEEAA+VYDNAAI+LRGPDALTNF+ PP
Sbjct: 127 FRGVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNFSIPP 186

Query: 186 PPPEKEAE-----------VNIPSVSGSYYDSGEES-HNLSSPTSVLHFRTQSPEESENP 245
              E+E E           +  P+ + S  +S EE   +LSSPTSVL+ R++  ++ + P
Sbjct: 187 QEEEEEEEPEPVIEEKPVIMTTPTPTTSSSESTEEDLQHLSSPTSVLNHRSEEIQQVQQP 246

Query: 246 PKPEAFQKPSPPSLADDQFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLF 305
            K     KP P       +H           ++T   + +D F L +P +  +F+E P  
Sbjct: 247 FKS---AKPEPGVSNAPWWHT---------GFNTGLGESDDSFPLDTPFLDNYFNESPP- 306

Query: 306 FEESIWNEGFSQIFTNMPE-------------DLGSPVLSSSISQGGDDY--FQDILLGS 330
            E SI+++   QIF    +             ++   + SSSI   G  +  F D L+ S
Sbjct: 307 -EMSIFDQPMDQIFCENDDIFNDMLFLGGETMNIEDELTSSSIKDMGSTFSDFDDSLI-S 353

[Close]

BLAST of MS000459 vs. ExPASy Swiss-Prot
Match: Q9SUQ2 (Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN=CRF2 PE=1 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 5.5e-40
Identity = 143/358 (39.94%), Postives = 196/358 (54.75%), Query Frame = 0


Query: 9   VKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGE----FFERQ 68
           +KFTEH+  T   +   +  N H   + R++RISVTDPDATDSSSD+EE E      +R+
Sbjct: 12  IKFTEHK--TNTTTIVSELTNTH---QTRILRISVTDPDATDSSSDDEEEEHQRFVSKRR 71

Query: 69  RIKKYVNEIKIESGSRNSLVPGC---------RKRPAGEGSEFRRPVKPPPTTNG-KKFR 128
           R+KK+VNE+ ++SG   ++V G          +KR     S     V    TT G KKFR
Sbjct: 72  RVKKFVNEVYLDSG---AVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFR 131

Query: 129 GVRQRPWGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFA----- 188
           GVRQRPWGKWAAEIRDP +R R+WLGT++TAEEAAMVYDNAAI+LRGPDALTNF+     
Sbjct: 132 GVRQRPWGKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTT 191

Query: 189 -----TPPPPP----EKEAEVNIPSVSGSYYDSGEESHN-LSSPTSVLHFRTQSPEESEN 248
                 PPP P    +K+   +  SV+ S   S   S++ L SP SVL       E S  
Sbjct: 192 ATEKKAPPPSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLRSPFAVDEFSGI 251

Query: 249 PPKPEAF----QKPSPPSLADDQFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFD 308
              P A     ++PS  ++++          +FS ++S      +D+F   S  +  +  
Sbjct: 252 SSSPVAAVVVKEEPSMTTVSE----------TFS-DFSAPLFSDDDVFDFRSSVVPDYLG 311

Query: 309 EQPLFFEESIWNEGFSQIFTNMPEDLGSPVLSSSISQGGDDYFQDI--LLGSDPLVVL 332
              LF E+    +  + +  N   D GS + S  +    +D+FQDI  L GSDPL+ +
Sbjct: 312 GD-LFGEDLFTADMCTDM--NFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

[Close]

BLAST of MS000459 vs. ExPASy Swiss-Prot
Match: O82503 (Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN=CRF1 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 7.9e-39
Identity = 135/308 (43.83%), Postives = 169/308 (54.87%), Query Frame = 0


Query: 37  RVVRISVTDPDATDSSSDEEEGEFFE-----RQRIKKYVNEIKIESGSRNSLVPGCRKRP 96
           R++RISVTDP ATDSSSDEEE   F+     R+R+KKYV E+ ++S   +   P  +KR 
Sbjct: 11  RILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVVSDKEKPMKKKRK 70

Query: 97  AGEGSEFRRPVKPP--PTTNGKKFRGVRQRPWGKWAAEIRDPARRQRVWLGTFDTAEEAA 156
                  +R V  P   TT  +KFRGVRQRPWGKWAAEIRDP+RR RVWLGTFDTAEEAA
Sbjct: 71  -------KRVVTVPVVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAA 130

Query: 157 MVYDNAAIKLRGPDALTNFATPPPPPE---KEAEVNIPSVSGSYYDSGEESHNLSSPTSV 216
           +VYDNAAI+LRGP+A  NF  PPPP     +EA   +  VS      GE    L SP SV
Sbjct: 131 IVYDNAAIQLRGPNAELNF--PPPPVTENVEEASTEVKGVSDFIIGGGE---CLRSPVSV 190

Query: 217 LHFRTQSPEESENPPKPEAFQKPSPPSLADDQFHECQGETSFSG-EYSTDFPKFEDIFQL 276
           L    +SP   E+    E F   S   +        + E SF+G ++S      +D+F  
Sbjct: 191 L----ESPFSGESTAVKEEFVGVSTAEIV------VKKEPSFNGSDFSAPLFSDDDVFGF 250

Query: 277 PSPDMSIFFDEQPLFFEESIWNEGFSQIFTNMPEDLGSPVLSSSISQGGDDYFQDI--LL 332
            S  MS  F    LF +    +  F   F       G    S   S   +D+FQDI  L 
Sbjct: 251 -STSMSESFGGD-LFGDNLFADMSFGSGF-------GFGSGSGFSSWHVEDHFQDIGDLF 287

[Close]

BLAST of MS000459 vs. ExPASy Swiss-Prot
Match: Q9M374 (Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN=CRF6 PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.3e-33
Identity = 100/219 (45.66%), Postives = 126/219 (57.53%), Query Frame = 0


Query: 9   VKFTEHRNFTKKFSTKKQQQNQHHPPEN---RVVRISVTDPDATDSSSDEEE-GEFFERQ 68
           VKFTE+R  T   +T          P N   R+VRI+VTDP ATDSSSD+++        
Sbjct: 8   VKFTENRTVTNVAAT----------PSNGSPRLVRITVTDPFATDSSSDDDDNNNVTVVP 67

Query: 69  RIKKYVNEIKIESGSRNSLVPGCRKRPAGEGS-----EFRRPVKPPPTTNGKKFRGVRQR 128
           R+K+YV EI+   G  +S     + +   E S     +    VKP      KK+RGVRQR
Sbjct: 68  RVKRYVKEIRFCQGESSSSTAARKGKHKEEESVVVEDDVSTSVKP------KKYRGVRQR 127

Query: 129 PWGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPPPEKEA 188
           PWGK+AAEIRDP+ R R+WLGTF TAEEAA+ YD AAI L+GP ALTNF TPP P     
Sbjct: 128 PWGKFAAEIRDPSSRTRIWLGTFVTAEEAAIAYDRAAIHLKGPKALTNFLTPPTP---TP 187

Query: 189 EVNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESE 219
            +++ +VS   Y   +   +L SPTSVL F      E E
Sbjct: 188 VIDLQTVSACDY-GRDSRQSLHSPTSVLRFNVNEETEHE 206

[Close]

BLAST of MS000459 vs. ExPASy TrEMBL
Match: A0A6J1CH70 (ethylene-responsive transcription factor CRF4-like OS=Momordica charantia OX=3673 GN=LOC111011618 PE=4 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 1.3e-188
Identity = 330/331 (99.70%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60
           MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF
Sbjct: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60

Query: 61  FERQRIKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120
           FERQR+KKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP
Sbjct: 61  FERQRVKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120

Query: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPPPEKEAE 180
           WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPPPEKEAE
Sbjct: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPPPEKEAE 180

Query: 181 VNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADDQFH 240
           VNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADDQFH
Sbjct: 181 VNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADDQFH 240

Query: 241 ECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNMPED 300
           ECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNMPED
Sbjct: 241 ECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNMPED 300

Query: 301 LGSPVLSSSISQGGDDYFQDILLGSDPLVVL 332
           LGSPVLSSSISQGGDDYFQDILLGSDPLVVL
Sbjct: 301 LGSPVLSSSISQGGDDYFQDILLGSDPLVVL 331

[Close]

BLAST of MS000459 vs. ExPASy TrEMBL
Match: A0A0A0LB30 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G357110 PE=4 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.4e-139
Identity = 258/334 (77.25%), Postives = 284/334 (85.03%), Query Frame = 0


Query: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60
           MEDTLF PVKFTEHRNFT KFS KK     HH  E+RVVRISVTDPDATDSSSDE+  +F
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKK-----HHQSESRVVRISVTDPDATDSSSDED--DF 60

Query: 61  FERQRIKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120
           FERQR+KKY+NEIKI+SG RN+L+P CRKRPAG+ SEFRR  K  P TNGKKFRGVRQRP
Sbjct: 61  FERQRVKKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRP 120

Query: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPPPEKEAE 180
           WGKWAAEIRDPARR R+WLGT+DTAEEAAMVYDNAAI+LRGPDALTNFATPPP P  E E
Sbjct: 121 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPPVPMPEKE 180

Query: 181 V---NIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADD 240
           V   NIPSVSGSYYDS EESHNLSSPTSVLHFRT SPEESE PPK +  QKP P    DD
Sbjct: 181 VETANIPSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKP-PAPFVDD 240

Query: 241 QFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNM 300
           QFHECQGETSF+ EY T+FP+F+  F+ PSP+  IF D+QPLFFE+SIWN+ FS+IFTN+
Sbjct: 241 QFHECQGETSFTEEYHTEFPRFDYDFKFPSPEAPIFLDDQPLFFEDSIWNDDFSEIFTNL 300

Query: 301 PEDLGSPVLSSSISQGGDDYFQDILLGSDPLVVL 332
           PED GSP+LSSSI QGGDDYFQDIL+GSDPLVVL
Sbjct: 301 PEDFGSPLLSSSIGQGGDDYFQDILMGSDPLVVL 326

[Close]

BLAST of MS000459 vs. ExPASy TrEMBL
Match: A0A5D3BJ86 (Ethylene-responsive transcription factor CRF4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold63471G00010 PE=4 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 4.5e-138
Identity = 260/335 (77.61%), Postives = 284/335 (84.78%), Query Frame = 0


Query: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60
           MEDTLF PVKFTEHRNFT KFS KK     HH  E+RVVRISVTDPDATDSSSDE+  +F
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKK-----HHQSESRVVRISVTDPDATDSSSDED--DF 60

Query: 61  FERQRIKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120
           FERQR+KKY+NEIKI+SG RN+L+P CRKRPAG+ SEFRR  K  P TNGKKFRGVRQRP
Sbjct: 61  FERQRVKKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRP 120

Query: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPP--PPPEKE 180
           WGKWAAEIRDPARR R+WLGT+DTAEEAAMVYDNAAI+LRGPDALTNFATPP  P PEKE
Sbjct: 121 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPAVPMPEKE 180

Query: 181 AE-VNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADD 240
            E  NIPSVSGSYYDS EESHNLSSPTSVLHFRT SPEESE PPK +  QKP P    DD
Sbjct: 181 VETTNIPSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKP-PAPFVDD 240

Query: 241 QFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNM 300
           QFHECQGETSFS EY T+FP+F+  F+ PSP+  IF D+QPLFFE+SIWN+ FS+IFTN+
Sbjct: 241 QFHECQGETSFSEEYHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNDDFSEIFTNL 300

Query: 301 PEDLGSPVL-SSSISQGGDDYFQDILLGSDPLVVL 332
           PED GSP+L SSSI QGGDDYFQDIL GSDPLVVL
Sbjct: 301 PEDFGSPLLSSSSIGQGGDDYFQDILTGSDPLVVL 327

[Close]

BLAST of MS000459 vs. ExPASy TrEMBL
Match: A0A1S3AY55 (ethylene-responsive transcription factor CRF4-like OS=Cucumis melo OX=3656 GN=LOC103484149 PE=4 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 4.5e-138
Identity = 260/335 (77.61%), Postives = 284/335 (84.78%), Query Frame = 0


Query: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60
           MEDTLF PVKFTEHRNFT KFS KK     HH  E+RVVRISVTDPDATDSSSDE+  +F
Sbjct: 1   MEDTLFSPVKFTEHRNFTNKFSAKK-----HHQSESRVVRISVTDPDATDSSSDED--DF 60

Query: 61  FERQRIKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120
           FERQR+KKY+NEIKI+SG RN+L+P CRKRPAG+ SEFRR  K  P TNGKKFRGVRQRP
Sbjct: 61  FERQRVKKYINEIKIQSGCRNNLLPSCRKRPAGDRSEFRRQGKVVPPTNGKKFRGVRQRP 120

Query: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPP--PPPEKE 180
           WGKWAAEIRDPARR R+WLGT+DTAEEAAMVYDNAAI+LRGPDALTNFATPP  P PEKE
Sbjct: 121 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPPAVPMPEKE 180

Query: 181 AE-VNIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADD 240
            E  NIPSVSGSYYDS EESHNLSSPTSVLHFRT SPEESE PPK +  QKP P    DD
Sbjct: 181 VETTNIPSVSGSYYDSSEESHNLSSPTSVLHFRTPSPEESEKPPKSDDLQKP-PAPFVDD 240

Query: 241 QFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNM 300
           QFHECQGETSFS EY T+FP+F+  F+ PSP+  IF D+QPLFFE+SIWN+ FS+IFTN+
Sbjct: 241 QFHECQGETSFSEEYHTEFPRFDYDFKFPSPESPIFLDDQPLFFEDSIWNDDFSEIFTNL 300

Query: 301 PEDLGSPVL-SSSISQGGDDYFQDILLGSDPLVVL 332
           PED GSP+L SSSI QGGDDYFQDIL GSDPLVVL
Sbjct: 301 PEDFGSPLLSSSSIGQGGDDYFQDILTGSDPLVVL 327

[Close]

BLAST of MS000459 vs. ExPASy TrEMBL
Match: A0A6J1E4E1 (ethylene-responsive transcription factor CRF4-like OS=Cucurbita moschata OX=3662 GN=LOC111430503 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.6e-135
Identity = 256/334 (76.65%), Postives = 281/334 (84.13%), Query Frame = 0


Query: 1   MEDTLFCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF 60
           MEDTLFCPVK TEHRNFT KFS KK     HH  E+RVVRISVTDPDATDSSSDEE  E 
Sbjct: 1   MEDTLFCPVKVTEHRNFTSKFSLKK-----HHQSESRVVRISVTDPDATDSSSDEE--EL 60

Query: 61  FERQRIKKYVNEIKIESGSRNSLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRP 120
           FERQR+KKY+NEIKI+SG RNSL+P CRKR AGE S+ RR  K  P TNGKKFRGVRQRP
Sbjct: 61  FERQRVKKYINEIKIQSGCRNSLLPSCRKRSAGEQSDSRRSGKVVPATNGKKFRGVRQRP 120

Query: 121 WGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPP--PPPEKE 180
           WGKWAAEIRDPARR R+WLGT+DTAEEAAMVYDNAAI+LRGPDALTNFATP   P PEKE
Sbjct: 121 WGKWAAEIRDPARRVRLWLGTYDTAEEAAMVYDNAAIQLRGPDALTNFATPSSVPMPEKE 180

Query: 181 AEV-NIPSVSGSYYDSGEESHNLSSPTSVLHFRTQSPEESENPPKPEAFQKPSPPSLADD 240
            EV NI SVSGSYYDS EESHNLSSPTSVLHFRT SPEE+E P KP+  Q+P PP  ADD
Sbjct: 181 VEVANIASVSGSYYDSSEESHNLSSPTSVLHFRTSSPEETEKPQKPDESQQP-PPPFADD 240

Query: 241 QFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIFTNM 300
           QFHE Q +T+ S EY T+FP+F+D+F+LP P+  IF DEQPLFFEESIWNE FS+ FTN+
Sbjct: 241 QFHERQVKTTLSEEYYTEFPRFDDVFKLPIPEPPIFVDEQPLFFEESIWNEDFSEFFTNL 300

Query: 301 PEDLGSPVLSSSISQGGDDYFQDILLGSDPLVVL 332
           PED G+P+LSS ISQGGDDYFQDIL+GSDPLVVL
Sbjct: 301 PEDFGTPLLSSPISQGGDDYFQDILMGSDPLVVL 326

[Close]

BLAST of MS000459 vs. TAIR 10
Match: AT4G27950.1 (cytokinin response factor 4 )

HSP 1 Score: 194.9 bits (494), Expect = 1.0e-49
Identity = 134/332 (40.36%), Postives = 199/332 (59.94%), Query Frame = 0


Query: 8   PVKFTEHRNFTKKFSTKKQQQNQHHPPEN-RVVRISVTDPDATDSSSDEEEGEFFERQRI 67
           PVK+TEH+   +K++ K   + +    ++ R+VR+S+TD DATDSSSDEEE   F R+R+
Sbjct: 11  PVKYTEHKTVIRKYTKKSSMERKTSVRDSARLVRVSMTDRDATDSSSDEEE-FLFPRRRV 70

Query: 68  KKYVNEIKIESGSRN----SLVPGCRKRPAGEGSEFRRPVKPPPTTNGKKFRGVRQRPWG 127
           K+ +NEI++E  S +    S  P   ++     S  ++P       N KK+RGVRQRPWG
Sbjct: 71  KRLINEIRVEPSSSSTGDVSASPTKDRKRINVDSTVQKP--SVSGQNQKKYRGVRQRPWG 130

Query: 128 KWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPPPPPEKEAEVN 187
           KWAAEIRDP +R+R+WLGTF TAEEAA+VYDNAAIKLRGPDALTNF   P P   + +  
Sbjct: 131 KWAAEIRDPEQRRRIWLGTFATAEEAAIVYDNAAIKLRGPDALTNFTVQPEPEPVQEQEQ 190

Query: 188 IP--SVSGSYYDSGEESHNLSSPTSVLHFRT---QSPEESENPPKPEAFQKPSPPSLADD 247
            P  ++S S  +S ++S +LSSPTSVL+++T   + P +S   P  + F +P    ++  
Sbjct: 191 EPESNMSVSISESMDDSQHLSSPTSVLNYQTYVSEEPIDSLIKPVKQEFLEPEQEPIS-- 250

Query: 248 QFHECQGETSFSGE-YSTDFPKFEDIFQLPSPDMSIFFDEQPLFFEESIWNEGFSQIF-- 307
            +H  +G T+ + + +  D    ++ F    PD+SIF  +QP+   +   N+ F+ +   
Sbjct: 251 -WHLGEGNTNTNDDSFPLDITFLDNYFNESLPDISIF--DQPMSPIQPTENDFFNDLMLF 310

Query: 308 -TNMPEDLGSPV--LSSSISQGGDDYFQDILL 324
            +N  E   S +  + SS +   D    D+LL
Sbjct: 311 DSNAEEYYSSEIKEIGSSFNDLDDSLISDLLL 334

[Close]

BLAST of MS000459 vs. TAIR 10
Match: AT5G53290.1 (cytokinin response factor 3 )

HSP 1 Score: 194.1 bits (492), Expect = 1.7e-49
Identity = 147/364 (40.38%), Postives = 201/364 (55.22%), Query Frame = 0


Query: 6   FCPVKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGEF-FERQ 65
           F P+K+TEH+    K+ TKK  +        + V I  TDPDATDSSSDE+E +F F R+
Sbjct: 7   FRPLKYTEHKTSMTKY-TKKSSEKLSGGKSLKKVSICYTDPDATDSSSDEDEEDFLFPRR 66

Query: 66  RIKKYVNEIKIESGSRNSLVPGC----RKRPAGEGSEFRRPVKPPPTTNG--------KK 125
           R+K++VNEI +E  S N++V G     RKR +    E + P       N         KK
Sbjct: 67  RVKRFVNEITVEP-SCNNVVTGVSMKDRKRLSSSSDETQSPASSRQRPNNKVSVSGQIKK 126

Query: 126 FRGVRQRPWGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFATPP 185
           FRGVRQRPWGKWAAEIRDP +R+R+WLGTF+TAEEAA+VYDNAAI+LRGPDALTNF+ PP
Sbjct: 127 FRGVRQRPWGKWAAEIRDPEQRRRIWLGTFETAEEAAVVYDNAAIRLRGPDALTNFSIPP 186

Query: 186 PPPEKEAE-----------VNIPSVSGSYYDSGEES-HNLSSPTSVLHFRTQSPEESENP 245
              E+E E           +  P+ + S  +S EE   +LSSPTSVL+ R++  ++ + P
Sbjct: 187 QEEEEEEEPEPVIEEKPVIMTTPTPTTSSSESTEEDLQHLSSPTSVLNHRSEEIQQVQQP 246

Query: 246 PKPEAFQKPSPPSLADDQFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFDEQPLF 305
            K     KP P       +H           ++T   + +D F L +P +  +F+E P  
Sbjct: 247 FKS---AKPEPGVSNAPWWHT---------GFNTGLGESDDSFPLDTPFLDNYFNESPP- 306

Query: 306 FEESIWNEGFSQIFTNMPE-------------DLGSPVLSSSISQGGDDY--FQDILLGS 330
            E SI+++   QIF    +             ++   + SSSI   G  +  F D L+ S
Sbjct: 307 -EMSIFDQPMDQIFCENDDIFNDMLFLGGETMNIEDELTSSSIKDMGSTFSDFDDSLI-S 353

[Close]

BLAST of MS000459 vs. TAIR 10
Match: AT4G23750.1 (cytokinin response factor 2 )

HSP 1 Score: 166.4 bits (420), Expect = 3.9e-41
Identity = 143/358 (39.94%), Postives = 196/358 (54.75%), Query Frame = 0


Query: 9   VKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGE----FFERQ 68
           +KFTEH+  T   +   +  N H   + R++RISVTDPDATDSSSD+EE E      +R+
Sbjct: 12  IKFTEHK--TNTTTIVSELTNTH---QTRILRISVTDPDATDSSSDDEEEEHQRFVSKRR 71

Query: 69  RIKKYVNEIKIESGSRNSLVPGC---------RKRPAGEGSEFRRPVKPPPTTNG-KKFR 128
           R+KK+VNE+ ++SG   ++V G          +KR     S     V    TT G KKFR
Sbjct: 72  RVKKFVNEVYLDSG---AVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFR 131

Query: 129 GVRQRPWGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFA----- 188
           GVRQRPWGKWAAEIRDP +R R+WLGT++TAEEAAMVYDNAAI+LRGPDALTNF+     
Sbjct: 132 GVRQRPWGKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTT 191

Query: 189 -----TPPPPP----EKEAEVNIPSVSGSYYDSGEESHN-LSSPTSVLHFRTQSPEESEN 248
                 PPP P    +K+   +  SV+ S   S   S++ L SP SVL       E S  
Sbjct: 192 ATEKKAPPPSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLRSPFAVDEFSGI 251

Query: 249 PPKPEAF----QKPSPPSLADDQFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFD 308
              P A     ++PS  ++++          +FS ++S      +D+F   S  +  +  
Sbjct: 252 SSSPVAAVVVKEEPSMTTVSE----------TFS-DFSAPLFSDDDVFDFRSSVVPDYLG 311

Query: 309 EQPLFFEESIWNEGFSQIFTNMPEDLGSPVLSSSISQGGDDYFQDI--LLGSDPLVVL 332
              LF E+    +  + +  N   D GS + S  +    +D+FQDI  L GSDPL+ +
Sbjct: 312 GD-LFGEDLFTADMCTDM--NFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

[Close]

BLAST of MS000459 vs. TAIR 10
Match: AT4G23750.2 (cytokinin response factor 2 )

HSP 1 Score: 166.4 bits (420), Expect = 3.9e-41
Identity = 143/358 (39.94%), Postives = 196/358 (54.75%), Query Frame = 0


Query: 9   VKFTEHRNFTKKFSTKKQQQNQHHPPENRVVRISVTDPDATDSSSDEEEGE----FFERQ 68
           +KFTEH+  T   +   +  N H   + R++RISVTDPDATDSSSD+EE E      +R+
Sbjct: 12  IKFTEHK--TNTTTIVSELTNTH---QTRILRISVTDPDATDSSSDDEEEEHQRFVSKRR 71

Query: 69  RIKKYVNEIKIESGSRNSLVPGC---------RKRPAGEGSEFRRPVKPPPTTNG-KKFR 128
           R+KK+VNE+ ++SG   ++V G          +KR     S     V    TT G KKFR
Sbjct: 72  RVKKFVNEVYLDSG---AVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFR 131

Query: 129 GVRQRPWGKWAAEIRDPARRQRVWLGTFDTAEEAAMVYDNAAIKLRGPDALTNFA----- 188
           GVRQRPWGKWAAEIRDP +R R+WLGT++TAEEAAMVYDNAAI+LRGPDALTNF+     
Sbjct: 132 GVRQRPWGKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTT 191

Query: 189 -----TPPPPP----EKEAEVNIPSVSGSYYDSGEESHN-LSSPTSVLHFRTQSPEESEN 248
                 PPP P    +K+   +  SV+ S   S   S++ L SP SVL       E S  
Sbjct: 192 ATEKKAPPPSPVKKKKKKNNKSKKSVTASSSISRSSSNDCLCSPVSVLRSPFAVDEFSGI 251

Query: 249 PPKPEAF----QKPSPPSLADDQFHECQGETSFSGEYSTDFPKFEDIFQLPSPDMSIFFD 308
              P A     ++PS  ++++          +FS ++S      +D+F   S  +  +  
Sbjct: 252 SSSPVAAVVVKEEPSMTTVSE----------TFS-DFSAPLFSDDDVFDFRSSVVPDYLG 311

Query: 309 EQPLFFEESIWNEGFSQIFTNMPEDLGSPVLSSSISQGGDDYFQDI--LLGSDPLVVL 332
              LF E+    +  + +  N   D GS + S  +    +D+FQDI  L GSDPL+ +
Sbjct: 312 GD-LFGEDLFTADMCTDM--NFGFDFGSGLSSWHM----EDHFQDIGDLFGSDPLLAV 343

[Close]

BLAST of MS000459 vs. TAIR 10
Match: AT4G11140.1 (cytokinin response factor 1 )

HSP 1 Score: 162.5 bits (410), Expect = 5.6e-40
Identity = 135/308 (43.83%), Postives = 169/308 (54.87%), Query Frame = 0


Query: 37  RVVRISVTDPDATDSSSDEEEGEFFE-----RQRIKKYVNEIKIESGSRNSLVPGCRKRP 96
           R++RISVTDP ATDSSSDEEE   F+     R+R+KKYV E+ ++S   +   P  +KR 
Sbjct: 11  RILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVVSDKEKPMKKKRK 70

Query: 97  AGEGSEFRRPVKPP--PTTNGKKFRGVRQRPWGKWAAEIRDPARRQRVWLGTFDTAEEAA 156
                  +R V  P   TT  +KFRGVRQRPWGKWAAEIRDP+RR RVWLGTFDTAEEAA
Sbjct: 71  -------KRVVTVPVVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAA 130

Query: 157 MVYDNAAIKLRGPDALTNFATPPPPPE---KEAEVNIPSVSGSYYDSGEESHNLSSPTSV 216
           +VYDNAAI+LRGP+A  NF  PPPP     +EA   +  VS      GE    L SP SV
Sbjct: 131 IVYDNAAIQLRGPNAELNF--PPPPVTENVEEASTEVKGVSDFIIGGGE---CLRSPVSV 190

Query: 217 LHFRTQSPEESENPPKPEAFQKPSPPSLADDQFHECQGETSFSG-EYSTDFPKFEDIFQL 276
           L    +SP   E+    E F   S   +        + E SF+G ++S      +D+F  
Sbjct: 191 L----ESPFSGESTAVKEEFVGVSTAEIV------VKKEPSFNGSDFSAPLFSDDDVFGF 250

Query: 277 PSPDMSIFFDEQPLFFEESIWNEGFSQIFTNMPEDLGSPVLSSSISQGGDDYFQDI--LL 332
            S  MS  F    LF +    +  F   F       G    S   S   +D+FQDI  L 
Sbjct: 251 -STSMSESFGGD-LFGDNLFADMSFGSGF-------GFGSGSGFSSWHVEDHFQDIGDLF 287

The following BLAST results are available for this feature:

BLAST of MS000459 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141160.1	2.6e-188	99.70	ethylene-responsive transcription factor CRF4-like [Momordica charantia]	[more]
XP_004149454.1	2.9e-139	77.25	ethylene-responsive transcription factor CRF4 [Cucumis sativus] >KGN57872.1 hypo...	[more]
XP_008439327.1	9.4e-138	77.61	PREDICTED: ethylene-responsive transcription factor CRF4-like [Cucumis melo] >KA...	[more]
XP_038900136.1	1.6e-137	76.95	ethylene-responsive transcription factor CRF4-like [Benincasa hispida]	[more]
KAG6576679.1	3.9e-136	76.95	Ethylene-responsive transcription factor CRF4, partial [Cucurbita argyrosperma s...	[more]

BLAST of MS000459 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SUE3	1.4e-48	40.36	Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9FK12	2.5e-48	40.38	Ethylene-responsive transcription factor CRF3 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9SUQ2	5.5e-40	39.94	Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O82503	7.9e-39	43.83	Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9M374	1.3e-33	45.66	Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of MS000459 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CH70	1.3e-188	99.70	ethylene-responsive transcription factor CRF4-like OS=Momordica charantia OX=367...	[more]
A0A0A0LB30	1.4e-139	77.25	AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G357110 PE=...	[more]
A0A5D3BJ86	4.5e-138	77.61	Ethylene-responsive transcription factor CRF4-like OS=Cucumis melo var. makuwa O...	[more]
A0A1S3AY55	4.5e-138	77.61	ethylene-responsive transcription factor CRF4-like OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1E4E1	3.6e-135	76.65	ethylene-responsive transcription factor CRF4-like OS=Cucurbita moschata OX=3662...	[more]

BLAST of MS000459 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27950.1	1.0e-49	40.36	cytokinin response factor 4	[more]
AT5G53290.1	1.7e-49	40.38	cytokinin response factor 3	[more]
AT4G23750.1	3.9e-41	39.94	cytokinin response factor 2	[more]
AT4G23750.2	3.9e-41	39.94	cytokinin response factor 2	[more]
AT4G11140.1	5.6e-40	43.83	cytokinin response factor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 113..124 score: 70.91 coord: 135..151 score: 67.06
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 112..175 e-value: 1.0E-39 score: 147.9
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 112..161 e-value: 3.3E-15 score: 56.0
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 112..169 score: 24.025661
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 111..169 e-value: 3.22969E-35 score: 120.454
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 111..170 e-value: 1.7E-27 score: 97.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 18..60
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 166..237
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 186..218
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 80..118
None	No IPR available	PANTHER	PTHR31194	SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR	coord: 6..221
None	No IPR available	PANTHER	PTHR31194:SF137	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR CRF4-LIKE	coord: 6..221
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 112..169

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000459.1	MS000459.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity