MS000262 (gene) Bitter gourd (TR) v1

Overview
NameMS000262
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionTyrosine--tRNA ligase
Locationscaffold44: 408711 .. 410195 (+)
RNA-Seq ExpressionMS000262
SyntenyMS000262
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTGCAACTATGGCGGCCATTGCTTCTGCTTGTGGCTCCAGAACCTTCCTTCTCTCCCAACGAAGCCTCTTCTTCTCCACCTTCAACATTTCCTCTAAGCCCACCTTCTCCTTCTCCTCCGTCAGAAGCAGTTGCCGCTGCATCGCTTCCTCTGCAAGTTCTCTCCAATCCTCCACTCTCGAACCCCACAGCGCTTCTCGGCGCAACGTCGTCGAAATTCTCGAAGAAAGAGGCCTTCTCGAGTCCATCACCAGCGACAATCTCCGCTCGGCTTCTCTCAGCCCTCTCAAGGTCTATTGCGGCTTCGACCCCACCGCCGAGAGCTTGCATTTGGGGAATCTCCTTGGTCTCATCGTTCTCTCTTGGTTCCGCCGATGCGGCCACGCTACCGTCGCTCTCATCGGTGGCGCCACCGCGCGAGTCGGCGACCCCTCCGGCAAGAGCCTGGAGCGGCCCGAGCTTGACCTCAAGACCTTGGAGGCCAACACTCTTGGAATTACCAATTCCATAACTAGAATTTTGGGGAATTCGGGCTCGGATTCGAATTTCAGCCCTAGCTTTGTGATTCTCAATAATTACGATTGGTGGAAGGAGTTCAGATTTCTGGATTTCTTGAAAGGAGTGGGTCGGTTTGCGAGAGTGGGGACGATGATGGCCAAGGAGAGCGTCAGGAGAAGGCTGGAAACGGAGCAAGGAATGAGCTATACTGAGTTTACTTACCAGCTCTTGCAGGCTTATGATTTTCTGCATCTGTTTCAAAACGAGGGTGTTTCTGTTCAGATTGGTGGTAGTGATCAATGGGGGAATATCACTGCAGGGACAGAGCTTATTAGGAAAATTTTGCCTCATGCTGATGGCGTTTATGGCTTAACGTTCCCTCTTCTGCTGAAGAGCGACGGCACCAAATTCGGCAAATCCGAAGACGGCGCCGTCTGGCTCTCGCCGTCAATGCTATCTCCATACAAGTTCTATCAGTACTTCTTCAGTGTCCCAGATGCTGATGTGGTCAGATTTCTCAAGATCCTCACATTTTTGGATATGAAGGACATTCAAGACTTGGAAAGCCAAATGGGGAGCTCCGGGTACGTGCCGAATACGGCGCAGAGGCGGCTGGCGGAAGAAGTTACCCGGTTTGTTCACGGAGAAGATGGTTTGAGTGAGGCCCTTAAGGCCACTGAGGCATTGAAACCAGGAGCAGAGACAAAGTTGGACTGGAAGACTATTGAGGGGATTGCTGAGGATGTTCCGTGCTGCTCATTGGGGTATGATCAAGTGTTGAATCTTTTGATTGTCGATCTTTCGGTTACTTCTGGTTTGCTCGAAAGCAAGTCAGCTGCCCGTCGATTGCTGAAGCAAGGGGGGCTTTATTTGAATAATAGTAGAGTGGATTCTGAAGGCAAGAGAGTTGAAGCTGCAGACATTGTAGATGGGAAGGTTCTGCTTCTATCTGCAGGAAAGAAGAACAAGGTGGTTGTAAGGATATTG

mRNA sequence

GCTGCAACTATGGCGGCCATTGCTTCTGCTTGTGGCTCCAGAACCTTCCTTCTCTCCCAACGAAGCCTCTTCTTCTCCACCTTCAACATTTCCTCTAAGCCCACCTTCTCCTTCTCCTCCGTCAGAAGCAGTTGCCGCTGCATCGCTTCCTCTGCAAGTTCTCTCCAATCCTCCACTCTCGAACCCCACAGCGCTTCTCGGCGCAACGTCGTCGAAATTCTCGAAGAAAGAGGCCTTCTCGAGTCCATCACCAGCGACAATCTCCGCTCGGCTTCTCTCAGCCCTCTCAAGGTCTATTGCGGCTTCGACCCCACCGCCGAGAGCTTGCATTTGGGGAATCTCCTTGGTCTCATCGTTCTCTCTTGGTTCCGCCGATGCGGCCACGCTACCGTCGCTCTCATCGGTGGCGCCACCGCGCGAGTCGGCGACCCCTCCGGCAAGAGCCTGGAGCGGCCCGAGCTTGACCTCAAGACCTTGGAGGCCAACACTCTTGGAATTACCAATTCCATAACTAGAATTTTGGGGAATTCGGGCTCGGATTCGAATTTCAGCCCTAGCTTTGTGATTCTCAATAATTACGATTGGTGGAAGGAGTTCAGATTTCTGGATTTCTTGAAAGGAGTGGGTCGGTTTGCGAGAGTGGGGACGATGATGGCCAAGGAGAGCGTCAGGAGAAGGCTGGAAACGGAGCAAGGAATGAGCTATACTGAGTTTACTTACCAGCTCTTGCAGGCTTATGATTTTCTGCATCTGTTTCAAAACGAGGGTGTTTCTGTTCAGATTGGTGGTAGTGATCAATGGGGGAATATCACTGCAGGGACAGAGCTTATTAGGAAAATTTTGCCTCATGCTGATGGCGTTTATGGCTTAACGTTCCCTCTTCTGCTGAAGAGCGACGGCACCAAATTCGGCAAATCCGAAGACGGCGCCGTCTGGCTCTCGCCGTCAATGCTATCTCCATACAAGTTCTATCAGTACTTCTTCAGTGTCCCAGATGCTGATGTGGTCAGATTTCTCAAGATCCTCACATTTTTGGATATGAAGGACATTCAAGACTTGGAAAGCCAAATGGGGAGCTCCGGGTACGTGCCGAATACGGCGCAGAGGCGGCTGGCGGAAGAAGTTACCCGGTTTGTTCACGGAGAAGATGGTTTGAGTGAGGCCCTTAAGGCCACTGAGGCATTGAAACCAGGAGCAGAGACAAAGTTGGACTGGAAGACTATTGAGGGGATTGCTGAGGATGTTCCGTGCTGCTCATTGGGGTATGATCAAGTGTTGAATCTTTTGATTGTCGATCTTTCGGTTACTTCTGGTTTGCTCGAAAGCAAGTCAGCTGCCCGTCGATTGCTGAAGCAAGGGGGGCTTTATTTGAATAATAGTAGAGTGGATTCTGAAGGCAAGAGAGTTGAAGCTGCAGACATTGTAGATGGGAAGGTTCTGCTTCTATCTGCAGGAAAGAAGAACAAGGTGGTTGTAAGGATATTG

Coding sequence (CDS)

GCTGCAACTATGGCGGCCATTGCTTCTGCTTGTGGCTCCAGAACCTTCCTTCTCTCCCAACGAAGCCTCTTCTTCTCCACCTTCAACATTTCCTCTAAGCCCACCTTCTCCTTCTCCTCCGTCAGAAGCAGTTGCCGCTGCATCGCTTCCTCTGCAAGTTCTCTCCAATCCTCCACTCTCGAACCCCACAGCGCTTCTCGGCGCAACGTCGTCGAAATTCTCGAAGAAAGAGGCCTTCTCGAGTCCATCACCAGCGACAATCTCCGCTCGGCTTCTCTCAGCCCTCTCAAGGTCTATTGCGGCTTCGACCCCACCGCCGAGAGCTTGCATTTGGGGAATCTCCTTGGTCTCATCGTTCTCTCTTGGTTCCGCCGATGCGGCCACGCTACCGTCGCTCTCATCGGTGGCGCCACCGCGCGAGTCGGCGACCCCTCCGGCAAGAGCCTGGAGCGGCCCGAGCTTGACCTCAAGACCTTGGAGGCCAACACTCTTGGAATTACCAATTCCATAACTAGAATTTTGGGGAATTCGGGCTCGGATTCGAATTTCAGCCCTAGCTTTGTGATTCTCAATAATTACGATTGGTGGAAGGAGTTCAGATTTCTGGATTTCTTGAAAGGAGTGGGTCGGTTTGCGAGAGTGGGGACGATGATGGCCAAGGAGAGCGTCAGGAGAAGGCTGGAAACGGAGCAAGGAATGAGCTATACTGAGTTTACTTACCAGCTCTTGCAGGCTTATGATTTTCTGCATCTGTTTCAAAACGAGGGTGTTTCTGTTCAGATTGGTGGTAGTGATCAATGGGGGAATATCACTGCAGGGACAGAGCTTATTAGGAAAATTTTGCCTCATGCTGATGGCGTTTATGGCTTAACGTTCCCTCTTCTGCTGAAGAGCGACGGCACCAAATTCGGCAAATCCGAAGACGGCGCCGTCTGGCTCTCGCCGTCAATGCTATCTCCATACAAGTTCTATCAGTACTTCTTCAGTGTCCCAGATGCTGATGTGGTCAGATTTCTCAAGATCCTCACATTTTTGGATATGAAGGACATTCAAGACTTGGAAAGCCAAATGGGGAGCTCCGGGTACGTGCCGAATACGGCGCAGAGGCGGCTGGCGGAAGAAGTTACCCGGTTTGTTCACGGAGAAGATGGTTTGAGTGAGGCCCTTAAGGCCACTGAGGCATTGAAACCAGGAGCAGAGACAAAGTTGGACTGGAAGACTATTGAGGGGATTGCTGAGGATGTTCCGTGCTGCTCATTGGGGTATGATCAAGTGTTGAATCTTTTGATTGTCGATCTTTCGGTTACTTCTGGTTTGCTCGAAAGCAAGTCAGCTGCCCGTCGATTGCTGAAGCAAGGGGGGCTTTATTTGAATAATAGTAGAGTGGATTCTGAAGGCAAGAGAGTTGAAGCTGCAGACATTGTAGATGGGAAGGTTCTGCTTCTATCTGCAGGAAAGAAGAACAAGGTGGTTGTAAGGATATTG

Protein sequence

AATMAAIASACGSRTFLLSQRSLFFSTFNISSKPTFSFSSVRSSCRCIASSASSLQSSTLEPHSASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWFRRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSDSNFSPSFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTYQLLQAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKFGKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSSGYVPNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLSAGKKNKVVVRIL
Homology
BLAST of MS000262 vs. NCBI nr
Match: XP_022141006.1 (tyrosine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia] >XP_022141007.1 tyrosine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 942.2 bits (2434), Expect = 1.8e-270
Identity = 485/491 (98.78%), Postives = 487/491 (99.19%), Query Frame = 0

Query: 4   MAAIASACGSRTFLLSQRSLFFSTFNISSKPTFSFSSVRSSCRCIASSASSLQSSTLEPH 63
           MAAIASACGSRTFLLSQRSLFFSTFNISSK T SFSSVRSSCRCIASSASSLQSSTLEPH
Sbjct: 1   MAAIASACGSRTFLLSQRSLFFSTFNISSKSTVSFSSVRSSCRCIASSASSLQSSTLEPH 60

Query: 64  SASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 123
           SASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF
Sbjct: 61  SASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 120

Query: 124 RRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSDSNF 183
           RRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSI RILGNSGSDSNF
Sbjct: 121 RRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSIARILGNSGSDSNF 180

Query: 184 SPSFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTYQLL 243
           SP+FVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLE+EQGMSYTEFTYQLL
Sbjct: 181 SPNFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLESEQGMSYTEFTYQLL 240

Query: 244 QAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKF 303
           QAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKF
Sbjct: 241 QAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKF 300

Query: 304 GKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSSGYV 363
           GKSEDGAVWLSPSMLSPYKFYQYFFSVPDADV RFLKILTFLDMKDIQDLESQMGSSGYV
Sbjct: 301 GKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVARFLKILTFLDMKDIQDLESQMGSSGYV 360

Query: 364 PNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYD 423
           PNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYD
Sbjct: 361 PNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYD 420

Query: 424 QVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLS 483
           QVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLS
Sbjct: 421 QVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLS 480

Query: 484 AGKKNKVVVRI 495
           AGKKNKVVVRI
Sbjct: 481 AGKKNKVVVRI 491

BLAST of MS000262 vs. NCBI nr
Match: KAG6600210.1 (Tyrosine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 836.6 bits (2160), Expect = 1.1e-238
Identity = 429/491 (87.37%), Postives = 458/491 (93.28%), Query Frame = 0

Query: 4   MAAIASACGSRTFLLSQRSLFFSTFNISSKPTFSFSSVRSSCRCIASSASSLQSSTLEPH 63
           MA  ASA  S TFLLSQR+LFFS   ISSKP+ SF S++S+  C+ASSASSLQSSTLEP 
Sbjct: 1   MAMAASARSSMTFLLSQRTLFFSNSIISSKPSLSF-SLKSTFSCLASSASSLQSSTLEPQ 60

Query: 64  SASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 123
           +ASRRNVVEILEERGLLESITSDNLRSA LSPLKVYCGFDPTAESLHLGNLLGLIVLSWF
Sbjct: 61  TASRRNVVEILEERGLLESITSDNLRSACLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 120

Query: 124 RRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSDSNF 183
           RRCGH TVALIGGATARVGDPSGKSLERPELDL+TLE NTLGITN ITRILGNSG DSNF
Sbjct: 121 RRCGHTTVALIGGATARVGDPSGKSLERPELDLQTLETNTLGITNIITRILGNSGLDSNF 180

Query: 184 SPSFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTYQLL 243
           S  FVILNNYDWWKEFRFLDFLK VGRFARVGTMMAKESV+RRLE++QGMSYTEFTYQLL
Sbjct: 181 SSDFVILNNYDWWKEFRFLDFLKEVGRFARVGTMMAKESVKRRLESDQGMSYTEFTYQLL 240

Query: 244 QAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKF 303
           Q YDFL+L+QN+GVSVQIGGSDQWGNITAGTELIRKILP ADGVYGLTFPLLLKSDGTKF
Sbjct: 241 QGYDFLYLYQNQGVSVQIGGSDQWGNITAGTELIRKILPQADGVYGLTFPLLLKSDGTKF 300

Query: 304 GKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSSGYV 363
           GKS DGA+WLSPS LSPYKFYQYFFSVPDADV+RFL+ILTFLD+++I+DLESQM S GYV
Sbjct: 301 GKSVDGAIWLSPSKLSPYKFYQYFFSVPDADVIRFLRILTFLDIEEIRDLESQMASPGYV 360

Query: 364 PNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYD 423
           PNTAQRRLAEEVTRFVHGEDGL+EAL+ATEALKPGAETKLDWKTI GI EDVPCCSL YD
Sbjct: 361 PNTAQRRLAEEVTRFVHGEDGLNEALEATEALKPGAETKLDWKTIVGIGEDVPCCSLEYD 420

Query: 424 QVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLS 483
           QVLNLL+VDL+V SGLLESKSAARRLLKQGGLYLNNSRVDSE K++EA DI+DGKVLLLS
Sbjct: 421 QVLNLLVVDLAVMSGLLESKSAARRLLKQGGLYLNNSRVDSESKKIEAIDIIDGKVLLLS 480

Query: 484 AGKKNKVVVRI 495
           AGKKNK+VVRI
Sbjct: 481 AGKKNKMVVRI 490

BLAST of MS000262 vs. NCBI nr
Match: XP_022942360.1 (tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 834.3 bits (2154), Expect = 5.4e-238
Identity = 428/491 (87.17%), Postives = 457/491 (93.08%), Query Frame = 0

Query: 4   MAAIASACGSRTFLLSQRSLFFSTFNISSKPTFSFSSVRSSCRCIASSASSLQSSTLEPH 63
           MA  ASA  S TFLLSQR+LFFS   ISSKP+ SF S++S+  C+ASSASSLQSSTLEP 
Sbjct: 1   MAMAASARSSMTFLLSQRTLFFSNSIISSKPSLSF-SLKSTFSCLASSASSLQSSTLEPQ 60

Query: 64  SASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 123
           +ASRRNVVEILEERGLLESITSDNLRSA LSPLKVYCGFDPTAESLHLGNLLGLIVLSWF
Sbjct: 61  TASRRNVVEILEERGLLESITSDNLRSACLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 120

Query: 124 RRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSDSNF 183
           RRCGH TVALIGGATARVGDPSGKSLERPELDL+TLE NTLGITN ITRILGNSG DSNF
Sbjct: 121 RRCGHTTVALIGGATARVGDPSGKSLERPELDLQTLETNTLGITNIITRILGNSGLDSNF 180

Query: 184 SPSFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTYQLL 243
           S  FVILNNYDWWKEFRFLDFLK VGRFARVGTMMAKESV+RRLE++QGMSYTEFTYQLL
Sbjct: 181 SSDFVILNNYDWWKEFRFLDFLKEVGRFARVGTMMAKESVKRRLESDQGMSYTEFTYQLL 240

Query: 244 QAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKF 303
           Q YDFL+L+QN+GVSVQIGGSDQWGNITAGTELIRKILP ADGVYGLTFPLLLKSDGTKF
Sbjct: 241 QGYDFLYLYQNQGVSVQIGGSDQWGNITAGTELIRKILPQADGVYGLTFPLLLKSDGTKF 300

Query: 304 GKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSSGYV 363
           GKS DGA+WLSPS LSPYKFYQYFFSVPDADV+RFL+ILTFLD+++I+DLESQM S GYV
Sbjct: 301 GKSVDGAIWLSPSKLSPYKFYQYFFSVPDADVIRFLRILTFLDIEEIRDLESQMASPGYV 360

Query: 364 PNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYD 423
           PNTAQRRLAEEVTRFVHGEDGL+EAL+ATEALKPGAETKLDWKTI GI EDVPCCSL YD
Sbjct: 361 PNTAQRRLAEEVTRFVHGEDGLNEALEATEALKPGAETKLDWKTIVGIGEDVPCCSLEYD 420

Query: 424 QVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLS 483
           QVLNL +VDL+V SGLLESKSAARRLLKQGGLYLNNSRVDSE K++EA DI+DGKVLLLS
Sbjct: 421 QVLNLSVVDLAVMSGLLESKSAARRLLKQGGLYLNNSRVDSESKKIEAIDIIDGKVLLLS 480

Query: 484 AGKKNKVVVRI 495
           AGKKNK+VVRI
Sbjct: 481 AGKKNKMVVRI 490

BLAST of MS000262 vs. NCBI nr
Match: KAG7030871.1 (Tyrosine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 832.4 bits (2149), Expect = 2.0e-237
Identity = 427/491 (86.97%), Postives = 457/491 (93.08%), Query Frame = 0

Query: 4   MAAIASACGSRTFLLSQRSLFFSTFNISSKPTFSFSSVRSSCRCIASSASSLQSSTLEPH 63
           MA  ASA  S TFLLSQR+LFFS   ISSKP+ SF S++S+  C+ASSASSLQSSTLEP 
Sbjct: 1   MAMAASARSSMTFLLSQRTLFFSNSIISSKPSLSF-SLKSTFSCLASSASSLQSSTLEPQ 60

Query: 64  SASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 123
           +ASRRNVVEILEERGLLESITSD+LRSA LSPLKVYCGFDPTAESLHLGNLLGLIVLSWF
Sbjct: 61  TASRRNVVEILEERGLLESITSDSLRSACLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 120

Query: 124 RRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSDSNF 183
           RRCGH TVALIGGATARVGDPSGKSLERPELDL+TLE NTLGITN ITRILGNSG DSNF
Sbjct: 121 RRCGHTTVALIGGATARVGDPSGKSLERPELDLQTLETNTLGITNIITRILGNSGLDSNF 180

Query: 184 SPSFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTYQLL 243
           S  FVILNNYDWWKEFRFLDFLK VGRFARVGTMMAKESV+RRLE++QGMSYTEFTYQLL
Sbjct: 181 SSDFVILNNYDWWKEFRFLDFLKEVGRFARVGTMMAKESVKRRLESDQGMSYTEFTYQLL 240

Query: 244 QAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKF 303
           Q YDFL+L+QN+GVSVQIGGSDQWGNITAGTELIRKILP ADGVYGLTFPLLLKSDGTKF
Sbjct: 241 QGYDFLYLYQNQGVSVQIGGSDQWGNITAGTELIRKILPQADGVYGLTFPLLLKSDGTKF 300

Query: 304 GKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSSGYV 363
           GKS DGA+WLSPS LSPYKFYQYFFSVPDADV+RFL+ILTFLD+++I+DLESQM S GYV
Sbjct: 301 GKSVDGAIWLSPSKLSPYKFYQYFFSVPDADVIRFLRILTFLDIEEIRDLESQMASPGYV 360

Query: 364 PNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYD 423
           PNTAQRRLAEEVTRFVHGEDGL+EAL+ATEALKPGAETKLDWKTI GI EDVPCCSL YD
Sbjct: 361 PNTAQRRLAEEVTRFVHGEDGLNEALEATEALKPGAETKLDWKTIVGIGEDVPCCSLEYD 420

Query: 424 QVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLS 483
           QVLNL +VDL+V SGLLESKSAARRLLKQGGLYLNNSRVDSE K++EA DI+DGKVLLLS
Sbjct: 421 QVLNLSVVDLAVMSGLLESKSAARRLLKQGGLYLNNSRVDSESKKIEAIDIIDGKVLLLS 480

Query: 484 AGKKNKVVVRI 495
           AGKKNK+VVRI
Sbjct: 481 AGKKNKMVVRI 490

BLAST of MS000262 vs. NCBI nr
Match: XP_023535379.1 (tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 831.6 bits (2147), Expect = 3.5e-237
Identity = 426/491 (86.76%), Postives = 458/491 (93.28%), Query Frame = 0

Query: 4   MAAIASACGSRTFLLSQRSLFFSTFNISSKPTFSFSSVRSSCRCIASSASSLQSSTLEPH 63
           MA  ASA  S TFLLSQR+LFFS   ISSKP+ +F S++S+  C+ASSASSLQSSTLEP 
Sbjct: 1   MAMAASARSSITFLLSQRTLFFSNSIISSKPSLTF-SLKSTFSCLASSASSLQSSTLEPQ 60

Query: 64  SASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 123
           +ASRRNVVEILEERGLLESITSDNLRSA LSPLKVYCGFDPTAESLHLGNLLGLIVLSWF
Sbjct: 61  TASRRNVVEILEERGLLESITSDNLRSACLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 120

Query: 124 RRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSDSNF 183
           RRCGH TVALIGGATARVGDPSGKSLERPELDL+TLE NTLGITN ITRILGNSG DSNF
Sbjct: 121 RRCGHTTVALIGGATARVGDPSGKSLERPELDLQTLETNTLGITNIITRILGNSGLDSNF 180

Query: 184 SPSFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTYQLL 243
           S +FVILNNYDWWKEFRFLDFLK VGRFARVGTMMAKESV+RRLE++QGMSYTEFTYQLL
Sbjct: 181 SSNFVILNNYDWWKEFRFLDFLKEVGRFARVGTMMAKESVKRRLESDQGMSYTEFTYQLL 240

Query: 244 QAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKF 303
           Q YDFL+L+QN+GVSVQIGGSDQWGNITAGTELIRKILP AD VYGLTFPLLLKSDGTKF
Sbjct: 241 QGYDFLYLYQNQGVSVQIGGSDQWGNITAGTELIRKILPQADAVYGLTFPLLLKSDGTKF 300

Query: 304 GKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSSGYV 363
           GKS DGA+WLSPS LSPYKFYQYFFSVPDADV+RFL+ILTFLD+++IQDLE+QM S+GYV
Sbjct: 301 GKSVDGAIWLSPSKLSPYKFYQYFFSVPDADVIRFLRILTFLDIEEIQDLENQMASAGYV 360

Query: 364 PNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYD 423
           PNTAQRRLAEEVTRFVHGEDGL+EAL+ATEALKPGAETKLDWKTI GI EDVPCCSL YD
Sbjct: 361 PNTAQRRLAEEVTRFVHGEDGLNEALEATEALKPGAETKLDWKTIVGIGEDVPCCSLEYD 420

Query: 424 QVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLS 483
           QVLNL +VDL+V SGLLESKSAARRLLKQGGLYLNNSRVDSE K++EA DI+DGKVLLLS
Sbjct: 421 QVLNLSVVDLAVMSGLLESKSAARRLLKQGGLYLNNSRVDSESKKIEAIDIIDGKVLLLS 480

Query: 484 AGKKNKVVVRI 495
           AGKKNK+VVRI
Sbjct: 481 AGKKNKMVVRI 490

BLAST of MS000262 vs. ExPASy Swiss-Prot
Match: Q9M876 (Tyrosine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=EMB2768 PE=2 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 2.6e-187
Identity = 346/498 (69.48%), Postives = 393/498 (78.92%), Query Frame = 0

Query: 13  SRTFLLSQRSLFFSTFNISSKPTF------------SFSSVRSSCRCIASSASSLQSSTL 72
           SRTFL   R      F   S  TF            S +++ SS +C   S SS ++   
Sbjct: 19  SRTFLSPLRVASLLVFPEKSSATFFRRVQVPHLFSTSTTTLFSSVKCSIHSTSSPET--- 78

Query: 73  EPHSASRRNVVEILEERGLLESITSDNLRSA----SLSPLKVYCGFDPTAESLHLGNLLG 132
           E  +  R NVV+ILEERGLLESITS+NLRSA     ++PL+VYCGFDPTAESLHLGNLLG
Sbjct: 79  ENQAVFRPNVVDILEERGLLESITSENLRSACSDPKVAPLRVYCGFDPTAESLHLGNLLG 138

Query: 133 LIVLSWFRRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGN 192
           +IVLSWF+RCGH  V LIGGAT RVGDPSGKSLERPELD  TLE N  GIT  I +ILG+
Sbjct: 139 IIVLSWFQRCGHQAVGLIGGATGRVGDPSGKSLERPELDADTLEKNIAGITKIIIKILGS 198

Query: 193 SGSDSNFSPSFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYT 252
           + S      S+VI NNYDWWK+   LDFL  VGRFARVGTMMAKESV++RLE+EQGMSYT
Sbjct: 199 NPSPGG---SYVIFNNYDWWKDMTMLDFLNKVGRFARVGTMMAKESVKKRLESEQGMSYT 258

Query: 253 EFTYQLLQAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLL 312
           EFTYQLLQAYDFLHLF+NEG++VQIGGSDQWGNITAGT+LIRKIL   +  YGLTFPLLL
Sbjct: 259 EFTYQLLQAYDFLHLFKNEGINVQIGGSDQWGNITAGTDLIRKILQAEEAAYGLTFPLLL 318

Query: 313 KSDGTKFGKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQ 372
           K+DGTKFGKSEDGA+WLSPSMLSPYKFYQYFFSVPD DV+RFLK LTFL + +I+ LE Q
Sbjct: 319 KNDGTKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDVDVIRFLKTLTFLSLDEIKILEDQ 378

Query: 373 MGSSGYVPNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVP 432
           M   GYVPNTAQ +LAEEVTRFVHGE+GL EA+KATEAL+PGAETKLDW  IE IAED+P
Sbjct: 379 MSKPGYVPNTAQIKLAEEVTRFVHGEEGLKEAIKATEALRPGAETKLDWNLIERIAEDIP 438

Query: 433 CCSLGYDQVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVD 492
            CSL  D+V  L IVDLSV++GL ESKSAARR+LKQGG Y+NN RVD E KRV+  DIV+
Sbjct: 439 SCSLPIDRVSGLSIVDLSVSAGLFESKSAARRMLKQGGFYMNNERVDDENKRVDEEDIVE 498

Query: 493 GKVLLLSAGKKNKVVVRI 495
           G+ L+LSAGKKNKVVVRI
Sbjct: 499 GRGLVLSAGKKNKVVVRI 510

BLAST of MS000262 vs. ExPASy Swiss-Prot
Match: Q6MC06 (Tyrosine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=tyrS PE=3 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 1.4e-132
Identity = 225/428 (52.57%), Postives = 315/428 (73.60%), Query Frame = 0

Query: 69  NVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWFRRCGH 128
           NV+++L ERG ++++TS+ +R  +  P+KVYCGFDPTA+SLHLGNL+ ++ L+WF+R GH
Sbjct: 3   NVMDVLRERGFIDAVTSEEIRQLTNQPIKVYCGFDPTADSLHLGNLVAIMGLAWFQRFGH 62

Query: 129 ATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSDSNF-SPSF 188
             VA++GGAT  +GDPSGK+ ER  LD K ++ N  GI+ ++  IL     D N  +   
Sbjct: 63  TPVAIVGGATGMIGDPSGKNAERQLLDEKKIQLNLKGISKNLEAIL-----DFNHPTAPA 122

Query: 189 VILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTYQLLQAYD 248
           +ILNN DW+K F F+ FL+ VG+  R+  M+AK+SV+ RL +E+GMS+TEF YQ+LQ YD
Sbjct: 123 IILNNLDWFKNFTFISFLRDVGKLFRLSPMLAKDSVKTRLNSEEGMSFTEFCYQILQGYD 182

Query: 249 FLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKFGKSE 308
           FLHLF+N GV+V++GGSDQWGNITAGT+LIRK+  HA   YG+TFPLL KSDG KFGKSE
Sbjct: 183 FLHLFENYGVTVELGGSDQWGNITAGTDLIRKV--HAKPAYGITFPLLTKSDGQKFGKSE 242

Query: 309 DGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSSGYVPNTA 368
            GAVWLSP  LS Y+FYQ+   V DADV+  +++LTFLDM +I+  E  M  + YVP TA
Sbjct: 243 KGAVWLSPDKLSSYEFYQHLIRVEDADVINLMRMLTFLDMGEIRHYEQMMKEADYVPRTA 302

Query: 369 QRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVLN 428
           Q+RLAEE+TR VHGE+GL  A+K TE + PG++T L+   +E +A D+P C +  + VLN
Sbjct: 303 QKRLAEEITRLVHGEEGLKIAIKVTEGVAPGSQTSLNADILEKLAADMPSCEMKLENVLN 362

Query: 429 LLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLSAGKK 488
             ++DL V + L  SKS ARRLL+ GG+Y+NN +++ E   +    ++  +++LL+AGKK
Sbjct: 363 KKLIDLLVETKLQTSKSEARRLLRNGGVYINNKKIEDENHIISVECLISSRLILLAAGKK 422

Query: 489 NKVVVRIL 496
           NK+++R++
Sbjct: 423 NKMIIRLM 423

BLAST of MS000262 vs. ExPASy Swiss-Prot
Match: A5UUZ9 (Tyrosine--tRNA ligase OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=tyrS PE=3 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 4.9e-93
Identity = 184/427 (43.09%), Postives = 271/427 (63.47%), Query Frame = 0

Query: 70  VVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWFRRCGHA 129
           +++ L  RGLL   +       +   + +Y GFDP+A+SLH+G+LL L+ L+ F+R GH 
Sbjct: 4   ILDELRWRGLLYESSDGLDELLTRERVTLYIGFDPSADSLHIGHLLPLLTLARFQRWGHT 63

Query: 130 TVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSDSNFSPSFVI 189
            +AL GG T  +GDPSGK+ ERP L  + + AN   I   + + L  +  D     S ++
Sbjct: 64  PIALAGGGTGLIGDPSGKTQERPLLSKEQVAANVEAIKGQLAQFLDFNAGDH----SALL 123

Query: 190 LNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTYQLLQAYDFL 249
           LNN DW      +DFL+ VG+   V  M+AK+SVR R+ +E G+S+TEF+Y LLQAYDF 
Sbjct: 124 LNNADWLTRLSLMDFLRDVGKHLTVNYMLAKDSVRSRMTSETGISFTEFSYMLLQAYDFA 183

Query: 250 HLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKFGKSEDG 309
           +LF++ G  +Q GGSDQWGNITAG ELIR+ L      YGL +PL+ K+DGTKFGK+E G
Sbjct: 184 YLFEHYGCKLQAGGSDQWGNITAGIELIRRTL--GQKAYGLVYPLVTKADGTKFGKTESG 243

Query: 310 AVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSSGYVPNTAQR 369
           AVWL P   SPY+FYQ++++V DADV ++L+  T+L   +I++LE ++ +S     TAQ+
Sbjct: 244 AVWLDPKRTSPYRFYQFWYNVDDADVGKYLRYFTWLSADEIEELE-RITASQPERRTAQQ 303

Query: 370 RLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVL--N 429
           RLA EVTR VHGE  L+ A +A++AL  G+  +L    +  I EDVP  +L         
Sbjct: 304 RLAREVTRMVHGETALARAEEASQALFGGSLARLSAGDVIDIFEDVPSTTLPRQDFEGDG 363

Query: 430 LLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLSAGKK 489
             ++D+ +  G+  SKS ARR ++ GG+Y+NN +    G+R+   D +DG+ ++L  G+K
Sbjct: 364 FPLIDVLLRCGIATSKSDARRTIEGGGIYVNNIQCPDVGRRLTLNDSIDGQFIVLRKGRK 423

Query: 490 NKVVVRI 495
              +V++
Sbjct: 424 QYHLVKV 423

BLAST of MS000262 vs. ExPASy Swiss-Prot
Match: A7NK64 (Tyrosine--tRNA ligase OS=Roseiflexus castenholzii (strain DSM 13941 / HLO8) OX=383372 GN=tyrS PE=3 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 3.2e-92
Identity = 185/429 (43.12%), Postives = 273/429 (63.64%), Query Frame = 0

Query: 70  VVEILEERGLLESITSDNLRS-ASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWFRRCGH 129
           +++ L  RGLL   +SD L        + +Y GFDP+A+SLH+G+LL L+ L+ F+R GH
Sbjct: 4   ILDELRWRGLLYE-SSDGLDDLLERERVTLYIGFDPSADSLHIGHLLPLLTLARFQRWGH 63

Query: 130 ATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSDSNFSPSFV 189
             +AL GG T  +GDPSGK+ ERP L  + + AN   I   + + L  +  D     S +
Sbjct: 64  TPIALAGGGTGLIGDPSGKTQERPLLSKEQVAANVEAIKRQLAQFLDFNAGDH----SAM 123

Query: 190 ILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTYQLLQAYDF 249
           +LNN +W      +DFL+ VG+   V  M+AK+SVR R+ +E G+S+TEF+Y LLQAYDF
Sbjct: 124 LLNNAEWLTRLSLMDFLRDVGKHLTVNYMLAKDSVRSRMMSETGISFTEFSYMLLQAYDF 183

Query: 250 LHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKFGKSED 309
            +LF++ G  +Q GGSDQWGNITAG ELIR+        YGL +PL+ K+DGTKFGK+E 
Sbjct: 184 AYLFEHYGCKLQAGGSDQWGNITAGIELIRR--TSGQKAYGLVYPLVTKADGTKFGKTES 243

Query: 310 GAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSSGYVPNTAQ 369
           GAVWL P   SPY+FYQ++++V DADV ++L+  T+L   +I++LE Q+ +      TAQ
Sbjct: 244 GAVWLDPKRTSPYRFYQFWYNVDDADVGKYLRYFTWLSAAEIEELE-QITARQPERRTAQ 303

Query: 370 RRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYDQVL-- 429
           +RLA EVTR VHGE  L+ A +A++AL  G+   L    +  I EDVP  +L    +   
Sbjct: 304 QRLAREVTRMVHGEAALARAEEASQALFGGSLASLSADDVADIFEDVPSITLPRQDLEGD 363

Query: 430 NLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLSAGK 489
            + ++D  V  G+  SKS ARR ++ GG+Y+NN +    G+R+   D +DG+ ++L  G+
Sbjct: 364 GMSLIDALVRCGIATSKSDARRAIEGGGIYINNVQSADVGRRLTLHDSIDGQCIVLRKGR 423

Query: 490 KNKVVVRIL 496
           ++  ++R++
Sbjct: 424 RHYHLIRLV 424

BLAST of MS000262 vs. ExPASy Swiss-Prot
Match: Q650K7 (Tyrosine--tRNA ligase OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=tyrS PE=3 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 1.6e-91
Identity = 188/432 (43.52%), Postives = 265/432 (61.34%), Query Frame = 0

Query: 69  NVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWFRRCGH 128
           N VE L  RG++  +        +   +  Y G DPTA+SLH+G+L G+++L  F+RCGH
Sbjct: 2   NFVEELRWRGMVHDMMPGTEELLAKEQVTAYVGIDPTADSLHIGHLCGVMILRHFQRCGH 61

Query: 129 ATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSDSNFSPSFV 188
             +ALIGGAT  +GDPSGKS ER  LD +TL  N   I   + + L       N +    
Sbjct: 62  KPLALIGGATGMIGDPSGKSAERNLLDEETLRHNQACIKKQLAKFLDFESDAPNRAE--- 121

Query: 189 ILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRL--ETEQGMSYTEFTYQLLQAY 248
           ++NNYDW KEF FLDF + VG+   V  MMAKESV++RL  E   G+S+TEFTYQLLQ Y
Sbjct: 122 LVNNYDWMKEFTFLDFAREVGKHITVNYMMAKESVKKRLNGEARDGLSFTEFTYQLLQGY 181

Query: 249 DFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKFGKS 308
           DFLHL++ +G  +Q+GGSDQWGNIT GTELIR+   +    Y LT PL+ K+DG KFGK+
Sbjct: 182 DFLHLYETKGCKLQMGGSDQWGNITTGTELIRR--TNGGEAYALTCPLITKADGGKFGKT 241

Query: 309 EDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSSGYVPNT 368
           E G +WL P   SPYKFYQ++ +V DAD  R++KI T LD  +I  L ++   + ++   
Sbjct: 242 ESGNIWLDPRYTSPYKFYQFWLNVSDADAERYIKIFTSLDKAEIDGLVAEHNEAPHL-RV 301

Query: 369 AQRRLAEEVTRFVHGEDGLSEALKATEALKPGAET----KLDWKTIEGIAEDVPCCSLGY 428
            Q+RLA+EVT  VH E+  + A+ A+  L   A +    KLD  T+  + E VP   +  
Sbjct: 302 LQKRLAKEVTVMVHSEEDYNAAVDASNILFGNATSDALKKLDEDTLLAVFEGVPQFEISR 361

Query: 429 DQVL-NLLIVDLSV-TSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVL 488
           D ++  +  VDL V  + +  SK   R+L++ GG+ LN  ++ +  + +  AD++D K L
Sbjct: 362 DALVEGVKAVDLFVDNAAVFASKGEMRKLVQGGGVSLNKEKLAAFDQVITTADLLDEKYL 421

Query: 489 LLSAGKKNKVVV 493
           L+  GKKN  ++
Sbjct: 422 LVQRGKKNYYLI 427

BLAST of MS000262 vs. ExPASy TrEMBL
Match: A0A6J1CIM7 (Tyrosine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111011519 PE=3 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 8.8e-271
Identity = 485/491 (98.78%), Postives = 487/491 (99.19%), Query Frame = 0

Query: 4   MAAIASACGSRTFLLSQRSLFFSTFNISSKPTFSFSSVRSSCRCIASSASSLQSSTLEPH 63
           MAAIASACGSRTFLLSQRSLFFSTFNISSK T SFSSVRSSCRCIASSASSLQSSTLEPH
Sbjct: 1   MAAIASACGSRTFLLSQRSLFFSTFNISSKSTVSFSSVRSSCRCIASSASSLQSSTLEPH 60

Query: 64  SASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 123
           SASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF
Sbjct: 61  SASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 120

Query: 124 RRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSDSNF 183
           RRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSI RILGNSGSDSNF
Sbjct: 121 RRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSIARILGNSGSDSNF 180

Query: 184 SPSFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTYQLL 243
           SP+FVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLE+EQGMSYTEFTYQLL
Sbjct: 181 SPNFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLESEQGMSYTEFTYQLL 240

Query: 244 QAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKF 303
           QAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKF
Sbjct: 241 QAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKF 300

Query: 304 GKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSSGYV 363
           GKSEDGAVWLSPSMLSPYKFYQYFFSVPDADV RFLKILTFLDMKDIQDLESQMGSSGYV
Sbjct: 301 GKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVARFLKILTFLDMKDIQDLESQMGSSGYV 360

Query: 364 PNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYD 423
           PNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYD
Sbjct: 361 PNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYD 420

Query: 424 QVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLS 483
           QVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLS
Sbjct: 421 QVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLS 480

Query: 484 AGKKNKVVVRI 495
           AGKKNKVVVRI
Sbjct: 481 AGKKNKVVVRI 491

BLAST of MS000262 vs. ExPASy TrEMBL
Match: A0A6J1FW55 (Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111447426 PE=3 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.6e-238
Identity = 428/491 (87.17%), Postives = 457/491 (93.08%), Query Frame = 0

Query: 4   MAAIASACGSRTFLLSQRSLFFSTFNISSKPTFSFSSVRSSCRCIASSASSLQSSTLEPH 63
           MA  ASA  S TFLLSQR+LFFS   ISSKP+ SF S++S+  C+ASSASSLQSSTLEP 
Sbjct: 1   MAMAASARSSMTFLLSQRTLFFSNSIISSKPSLSF-SLKSTFSCLASSASSLQSSTLEPQ 60

Query: 64  SASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 123
           +ASRRNVVEILEERGLLESITSDNLRSA LSPLKVYCGFDPTAESLHLGNLLGLIVLSWF
Sbjct: 61  TASRRNVVEILEERGLLESITSDNLRSACLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 120

Query: 124 RRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSDSNF 183
           RRCGH TVALIGGATARVGDPSGKSLERPELDL+TLE NTLGITN ITRILGNSG DSNF
Sbjct: 121 RRCGHTTVALIGGATARVGDPSGKSLERPELDLQTLETNTLGITNIITRILGNSGLDSNF 180

Query: 184 SPSFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTYQLL 243
           S  FVILNNYDWWKEFRFLDFLK VGRFARVGTMMAKESV+RRLE++QGMSYTEFTYQLL
Sbjct: 181 SSDFVILNNYDWWKEFRFLDFLKEVGRFARVGTMMAKESVKRRLESDQGMSYTEFTYQLL 240

Query: 244 QAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKF 303
           Q YDFL+L+QN+GVSVQIGGSDQWGNITAGTELIRKILP ADGVYGLTFPLLLKSDGTKF
Sbjct: 241 QGYDFLYLYQNQGVSVQIGGSDQWGNITAGTELIRKILPQADGVYGLTFPLLLKSDGTKF 300

Query: 304 GKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSSGYV 363
           GKS DGA+WLSPS LSPYKFYQYFFSVPDADV+RFL+ILTFLD+++I+DLESQM S GYV
Sbjct: 301 GKSVDGAIWLSPSKLSPYKFYQYFFSVPDADVIRFLRILTFLDIEEIRDLESQMASPGYV 360

Query: 364 PNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYD 423
           PNTAQRRLAEEVTRFVHGEDGL+EAL+ATEALKPGAETKLDWKTI GI EDVPCCSL YD
Sbjct: 361 PNTAQRRLAEEVTRFVHGEDGLNEALEATEALKPGAETKLDWKTIVGIGEDVPCCSLEYD 420

Query: 424 QVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLS 483
           QVLNL +VDL+V SGLLESKSAARRLLKQGGLYLNNSRVDSE K++EA DI+DGKVLLLS
Sbjct: 421 QVLNLSVVDLAVMSGLLESKSAARRLLKQGGLYLNNSRVDSESKKIEAIDIIDGKVLLLS 480

Query: 484 AGKKNKVVVRI 495
           AGKKNK+VVRI
Sbjct: 481 AGKKNKMVVRI 490

BLAST of MS000262 vs. ExPASy TrEMBL
Match: A0A6J1JFS2 (Tyrosine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111486472 PE=3 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 2.1e-235
Identity = 424/491 (86.35%), Postives = 454/491 (92.46%), Query Frame = 0

Query: 4   MAAIASACGSRTFLLSQRSLFFSTFNISSKPTFSFSSVRSSCRCIASSASSLQSSTLEPH 63
           MA  ASA  S TFLLSQR+LFFS   ISSKP+ SF S++S+  C+ASSASSLQSSTLEP 
Sbjct: 1   MAMAASARSSITFLLSQRTLFFSNSIISSKPSLSF-SLKSTFSCLASSASSLQSSTLEPQ 60

Query: 64  SASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 123
           +ASRRNVVEILEERGLLESITSDNLR A LSPLKVYCGFDPTAESLHLGNLLGLIVLSWF
Sbjct: 61  TASRRNVVEILEERGLLESITSDNLRFACLSPLKVYCGFDPTAESLHLGNLLGLIVLSWF 120

Query: 124 RRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSDSNF 183
           RRCGH +VALIGGATARVGDPSGKSLERPELDL+TLE NTLGITN ITRILGNSG DSNF
Sbjct: 121 RRCGHTSVALIGGATARVGDPSGKSLERPELDLQTLETNTLGITNIITRILGNSGLDSNF 180

Query: 184 SPSFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTYQLL 243
           S  FVILNNYDWWKEFRFLDFLK VGRFARVGTMMAKESV+RRLE++QGMSYTEFTYQLL
Sbjct: 181 SSDFVILNNYDWWKEFRFLDFLKEVGRFARVGTMMAKESVKRRLESDQGMSYTEFTYQLL 240

Query: 244 QAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDGTKF 303
           Q YDFL+L+QN+GV VQIGGSDQWGNITAGTELIRKILP AD VYGLTFPLLLKSDGTKF
Sbjct: 241 QGYDFLYLYQNQGVCVQIGGSDQWGNITAGTELIRKILPQADAVYGLTFPLLLKSDGTKF 300

Query: 304 GKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSSGYV 363
           GKS DGA+WLSPS LSPYKFYQYFFSVPDADV+RFL+ILTFLD+++I+DLESQM S GYV
Sbjct: 301 GKSVDGAIWLSPSKLSPYKFYQYFFSVPDADVIRFLRILTFLDIEEIRDLESQMVSPGYV 360

Query: 364 PNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSLGYD 423
           PNTAQRRLAEEVTRFVHGEDGL+EAL+ATEALKPGAETKLDWKTI GI EDVPCCSL YD
Sbjct: 361 PNTAQRRLAEEVTRFVHGEDGLNEALEATEALKPGAETKLDWKTIVGIGEDVPCCSLEYD 420

Query: 424 QVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVLLLS 483
           QVLNL +VDL+V SGLLESKSAARRLLKQGGLYLNNSRVDSE K++EA DI+DGKVLLLS
Sbjct: 421 QVLNLSVVDLAVMSGLLESKSAARRLLKQGGLYLNNSRVDSESKKIEAVDIIDGKVLLLS 480

Query: 484 AGKKNKVVVRI 495
           AGKKNK+VVRI
Sbjct: 481 AGKKNKMVVRI 490

BLAST of MS000262 vs. ExPASy TrEMBL
Match: A0A0A0KU70 (Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_4G025150 PE=3 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 2.2e-229
Identity = 421/494 (85.22%), Postives = 450/494 (91.09%), Query Frame = 0

Query: 2   ATMAAIASAC-GSRTFLLSQRSLFFSTFNISSKPTFSFSSVRSSCRCIASSASSLQSSTL 61
           A  A+ +S+C  S+ FLLSQRS FFST  ISSKP+ SF    +S    +SS +SL  S  
Sbjct: 4   AIFASSSSSCYTSKAFLLSQRSFFFST--ISSKPSSSFFFKPTSRFFSSSSPTSLHYS-- 63

Query: 62  EPHSASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVL 121
            P  +   NV++ILE+RGLL+SITSDNLRSASLSPLKVYCGFDPTA+SLHLGNLLGLIVL
Sbjct: 64  -PTPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVL 123

Query: 122 SWFRRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSD 181
           SWFRRCGH+TVALIGGATARVGDPSGKSLERPELDL+TLEANTLGITN IT+ILGNSGSD
Sbjct: 124 SWFRRCGHSTVALIGGATARVGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSD 183

Query: 182 SNFSPSFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTY 241
           S+F P+FVILNNYDWWKEFRFLDFLK VGRFARVG+M+AKESVRRRLE+EQGMSYTEFTY
Sbjct: 184 SDFCPNFVILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTY 243

Query: 242 QLLQAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDG 301
           QLLQ YDFLHL+QNEGVSVQIGGSDQWGNITAGTELIRK LP ADGVYGLTFPLLLKSDG
Sbjct: 244 QLLQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDG 303

Query: 302 TKFGKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSS 361
           TKFGKSEDGA+WLSPSMLSPYKFYQYFFSVPDADVVRFL+ILTFLD+++IQDLESQM   
Sbjct: 304 TKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMMKP 363

Query: 362 GYVPNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSL 421
           GYVPNTAQRRLAEEVTRFVHGEDGL EALKATEALKPGAETKLDWKTIEGIAEDVPCCSL
Sbjct: 364 GYVPNTAQRRLAEEVTRFVHGEDGLREALKATEALKPGAETKLDWKTIEGIAEDVPCCSL 423

Query: 422 GYDQVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVL 481
            YDQVLNL +VDLSVTSGLLESKSAARRLLKQGGLYLNN RVDSE KRVEA DIVDGKVL
Sbjct: 424 EYDQVLNLSVVDLSVTSGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDIVDGKVL 483

Query: 482 LLSAGKKNKVVVRI 495
           LLSAGKKNKVVVRI
Sbjct: 484 LLSAGKKNKVVVRI 492

BLAST of MS000262 vs. ExPASy TrEMBL
Match: A0A5D3CZD9 (Tyrosine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00290 PE=3 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 7.1e-228
Identity = 414/494 (83.81%), Postives = 451/494 (91.30%), Query Frame = 0

Query: 2   ATMAAIASAC-GSRTFLLSQRSLFFSTFNISSKPTFSFSSVRSSCRCIASSASSLQSSTL 61
           A +A+ + +C  S+ FLLSQRS FFST  ISSKP+ SF    +S    +SS +SL+ S  
Sbjct: 4   AILASSSPSCYTSKAFLLSQRSFFFST--ISSKPSSSFFLKPTSRFFASSSPTSLRYS-- 63

Query: 62  EPHSASRRNVVEILEERGLLESITSDNLRSASLSPLKVYCGFDPTAESLHLGNLLGLIVL 121
            P  +   NV++ILE+RGLL+SITSDNLRSASLSPLKVYCGFDPTA+SLHLGNLLGLIVL
Sbjct: 64  -PTPSPPTNVIQILEQRGLLDSITSDNLRSASLSPLKVYCGFDPTAQSLHLGNLLGLIVL 123

Query: 122 SWFRRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGNSGSD 181
           SWFRRCGH+TVALIGGATAR+GDPSGKSLERPELDL+TLEANTLGITN IT+ILGNSGSD
Sbjct: 124 SWFRRCGHSTVALIGGATARIGDPSGKSLERPELDLQTLEANTLGITNIITKILGNSGSD 183

Query: 182 SNFSPSFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYTEFTY 241
           S+F P+F+ILNNYDWWKEFRFLDFLK VGRFARVG+M+AKESVRRRLE+EQGMSYTEFTY
Sbjct: 184 SDFCPNFLILNNYDWWKEFRFLDFLKDVGRFARVGSMIAKESVRRRLESEQGMSYTEFTY 243

Query: 242 QLLQAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLLKSDG 301
           QLLQ YDFLHL+QNEGVSVQIGGSDQWGNITAGTELIRK LP ADGVYGLTFPLLLKSDG
Sbjct: 244 QLLQGYDFLHLYQNEGVSVQIGGSDQWGNITAGTELIRKRLPQADGVYGLTFPLLLKSDG 303

Query: 302 TKFGKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQMGSS 361
           TKFGKSEDGA+WLSPSMLSPYKFYQYFFSVPDADVVRFL+ILTFLD+++IQDLESQM   
Sbjct: 304 TKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDADVVRFLRILTFLDIEEIQDLESQMTKP 363

Query: 362 GYVPNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSL 421
           GYVPNT QRRLAEEVTRFVHGE+GLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSL
Sbjct: 364 GYVPNTVQRRLAEEVTRFVHGENGLSEALKATEALKPGAETKLDWKTIEGIAEDVPCCSL 423

Query: 422 GYDQVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVDGKVL 481
            YDQVLNL +VDLSVT+GLLESKSAARRLLKQGGLYLNN RVDSE KRVEA D++DGKVL
Sbjct: 424 EYDQVLNLSVVDLSVTTGLLESKSAARRLLKQGGLYLNNGRVDSESKRVEAVDVIDGKVL 483

Query: 482 LLSAGKKNKVVVRI 495
           LLSAGKKNKVVVRI
Sbjct: 484 LLSAGKKNKVVVRI 492

BLAST of MS000262 vs. TAIR 10
Match: AT3G02660.1 (Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial )

HSP 1 Score: 656.4 bits (1692), Expect = 1.9e-188
Identity = 346/498 (69.48%), Postives = 393/498 (78.92%), Query Frame = 0

Query: 13  SRTFLLSQRSLFFSTFNISSKPTF------------SFSSVRSSCRCIASSASSLQSSTL 72
           SRTFL   R      F   S  TF            S +++ SS +C   S SS ++   
Sbjct: 19  SRTFLSPLRVASLLVFPEKSSATFFRRVQVPHLFSTSTTTLFSSVKCSIHSTSSPET--- 78

Query: 73  EPHSASRRNVVEILEERGLLESITSDNLRSA----SLSPLKVYCGFDPTAESLHLGNLLG 132
           E  +  R NVV+ILEERGLLESITS+NLRSA     ++PL+VYCGFDPTAESLHLGNLLG
Sbjct: 79  ENQAVFRPNVVDILEERGLLESITSENLRSACSDPKVAPLRVYCGFDPTAESLHLGNLLG 138

Query: 133 LIVLSWFRRCGHATVALIGGATARVGDPSGKSLERPELDLKTLEANTLGITNSITRILGN 192
           +IVLSWF+RCGH  V LIGGAT RVGDPSGKSLERPELD  TLE N  GIT  I +ILG+
Sbjct: 139 IIVLSWFQRCGHQAVGLIGGATGRVGDPSGKSLERPELDADTLEKNIAGITKIIIKILGS 198

Query: 193 SGSDSNFSPSFVILNNYDWWKEFRFLDFLKGVGRFARVGTMMAKESVRRRLETEQGMSYT 252
           + S      S+VI NNYDWWK+   LDFL  VGRFARVGTMMAKESV++RLE+EQGMSYT
Sbjct: 199 NPSPGG---SYVIFNNYDWWKDMTMLDFLNKVGRFARVGTMMAKESVKKRLESEQGMSYT 258

Query: 253 EFTYQLLQAYDFLHLFQNEGVSVQIGGSDQWGNITAGTELIRKILPHADGVYGLTFPLLL 312
           EFTYQLLQAYDFLHLF+NEG++VQIGGSDQWGNITAGT+LIRKIL   +  YGLTFPLLL
Sbjct: 259 EFTYQLLQAYDFLHLFKNEGINVQIGGSDQWGNITAGTDLIRKILQAEEAAYGLTFPLLL 318

Query: 313 KSDGTKFGKSEDGAVWLSPSMLSPYKFYQYFFSVPDADVVRFLKILTFLDMKDIQDLESQ 372
           K+DGTKFGKSEDGA+WLSPSMLSPYKFYQYFFSVPD DV+RFLK LTFL + +I+ LE Q
Sbjct: 319 KNDGTKFGKSEDGAIWLSPSMLSPYKFYQYFFSVPDVDVIRFLKTLTFLSLDEIKILEDQ 378

Query: 373 MGSSGYVPNTAQRRLAEEVTRFVHGEDGLSEALKATEALKPGAETKLDWKTIEGIAEDVP 432
           M   GYVPNTAQ +LAEEVTRFVHGE+GL EA+KATEAL+PGAETKLDW  IE IAED+P
Sbjct: 379 MSKPGYVPNTAQIKLAEEVTRFVHGEEGLKEAIKATEALRPGAETKLDWNLIERIAEDIP 438

Query: 433 CCSLGYDQVLNLLIVDLSVTSGLLESKSAARRLLKQGGLYLNNSRVDSEGKRVEAADIVD 492
            CSL  D+V  L IVDLSV++GL ESKSAARR+LKQGG Y+NN RVD E KRV+  DIV+
Sbjct: 439 SCSLPIDRVSGLSIVDLSVSAGLFESKSAARRMLKQGGFYMNNERVDDENKRVDEEDIVE 498

Query: 493 GKVLLLSAGKKNKVVVRI 495
           G+ L+LSAGKKNKVVVRI
Sbjct: 499 GRGLVLSAGKKNKVVVRI 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141006.11.8e-27098.78tyrosine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia] >XP_022... [more]
KAG6600210.11.1e-23887.37Tyrosine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrospe... [more]
XP_022942360.15.4e-23887.17tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata][more]
KAG7030871.12.0e-23786.97Tyrosine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrospe... [more]
XP_023535379.13.5e-23786.76tyrosine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9M8762.6e-18769.48Tyrosine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=37... [more]
Q6MC061.4e-13252.57Tyrosine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=... [more]
A5UUZ94.9e-9343.09Tyrosine--tRNA ligase OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=tyrS PE=3 SV... [more]
A7NK643.2e-9243.12Tyrosine--tRNA ligase OS=Roseiflexus castenholzii (strain DSM 13941 / HLO8) OX=3... [more]
Q650K71.6e-9143.52Tyrosine--tRNA ligase OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=tyrS P... [more]
Match NameE-valueIdentityDescription
A0A6J1CIM78.8e-27198.78Tyrosine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111011519 PE=3 SV=1[more]
A0A6J1FW552.6e-23887.17Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111447426 PE=3 SV=1[more]
A0A6J1JFS22.1e-23586.35Tyrosine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111486472 PE=3 SV=1[more]
A0A0A0KU702.2e-22985.22Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_4G025150 PE=3 SV=1[more]
A0A5D3CZD97.1e-22883.81Tyrosine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13... [more]
Match NameE-valueIdentityDescription
AT3G02660.11.9e-18869.48Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002307Tyrosine-tRNA ligasePRINTSPR01040TRNASYNTHTYRcoord: 232..247
score: 53.12
coord: 288..300
score: 46.92
coord: 253..275
score: 47.25
coord: 108..130
score: 34.35
IPR002307Tyrosine-tRNA ligaseTIGRFAMTIGR00234TIGR00234coord: 69..492
e-value: 2.2E-104
score: 347.7
IPR002307Tyrosine-tRNA ligaseCDDcd00805TyrRS_corecoord: 96..378
e-value: 1.27762E-106
score: 316.855
IPR002305Aminoacyl-tRNA synthetase, class IcPFAMPF00579tRNA-synt_1bcoord: 94..394
e-value: 7.4E-71
score: 238.9
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 40..294
e-value: 1.9E-94
score: 318.1
IPR036986RNA-binding S4 domain superfamilyGENE3D3.10.290.10coord: 400..494
e-value: 3.9E-21
score: 76.7
NoneNo IPR availableGENE3D1.10.240.10coord: 295..399
e-value: 7.8E-35
score: 121.3
NoneNo IPR availablePROSITEPS50889S4coord: 428..494
score: 11.559733
NoneNo IPR availableSUPERFAMILY55174Alpha-L RNA-binding motifcoord: 397..493
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 69..394
IPR002942RNA-binding S4 domainPFAMPF01479S4coord: 436..475
e-value: 2.5E-6
score: 27.1
IPR002942RNA-binding S4 domainCDDcd00165S4coord: 435..494
e-value: 3.87137E-7
score: 45.3182
IPR024088Tyrosine-tRNA ligase, bacterial-typePANTHERPTHR11766TYROSYL-TRNA SYNTHETASEcoord: 66..494
IPR001412Aminoacyl-tRNA synthetase, class I, conserved sitePROSITEPS00178AA_TRNA_LIGASE_Icoord: 104..114
IPR024107Tyrosine-tRNA ligase, bacterial-type, type 1HAMAPMF_02006Tyr_tRNA_synth_type1coord: 68..494
score: 39.894012

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000262.1MS000262.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006418 tRNA aminoacylation for protein translation
biological_process GO:0006437 tyrosyl-tRNA aminoacylation
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0004831 tyrosine-tRNA ligase activity