MELO3C035704 (gene) Melon (DHL92) v4

Overview
NameMELO3C035704
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptiontRNA-5-taurinomethyluridine 2-sulfurtransferase
Locationchr12: 19695810 .. 19698329 (-)
RNA-Seq ExpressionMELO3C035704
SyntenyMELO3C035704
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCTCCATGTTTTATTCGAGCCAATGTGTAGAAAATGGCAAGGCCTCTTTTGGGGTTGTTGAAGCCGTTAACCTTATAACTTCTTTGAAATTATTTGGTTTACTCTTGGTTGTTGGCTATCCGGTATCAGTAGGTGTTAGTCTTTATTAATCCTATTAAAGTGTATATGTTTGATTCAGTGTTAGTTAATTTATAGATTGTGAGATGAGCATTAGAATCCACATGTATGAGTATGTGTATAGCTTGTGCAATTACAAAGACTCTTTTGTTGGAAATGAAACTAAAACACTGCTTACTATTCATATTCATCTCAAACTCATTTATAAACTTCGATAAAGACAAACAAAAAGTCCAACAATGTATCTCATCACTTTGTCAAAACAAATTCAGATAATATTAAACATTGATTGATGAAGCTCAACAATTAAACCAGATAACTATTTTCAAGGAGGACCAAAATGAAGCAAATTGGAAAAGGAAAAAAAAGAAAAGGATCAAAGCAACGGTGACTGTTTCATAGTGGATCACCTGGGCACTCACAAACTTGTTTGTGAAATGCATCTTCAAGACATGGCTTTTAGATACTTGGTTATGGACTTAAGGAATGCAATTTTAGATGGATGTGCTGTAGATTGTAATGTCAAGATATGTTAAACTTGAATATTATGTGATGACTTTTGACAAGATATGTTCAATTTTTTAACCGGGTTTTTAGCATATTGAAATATGCAACTGTGCAAGCAGAGTTTTTACTCATGTTGATTATATAATACTGCTGTTGTATATTTCCATGATGTTTTATTACCACAAAACTTGTATGCATAAAAATTGTTTGTAATACATATCCGATCCCTTAGACTCAACTACATACGTAGTGGATAAAGGTCTTCAAGCATAAAATAGGCATCTTAAATTTTATGCTATTGGTTGAATTGAACTTGACCGTCGTTTGAATTGCTCGGTAGAACCTGTAGGACTACATTAAGATGGGGTTCTACACTTTTTCTTCGGCCATTTCAAATTTCATTAGAACTAAGATATCCTCCTGGGACATGCGTTTTAAGTTCAGGGTTTTTGTTATTGATTGAACTTGACAGCCTTTTACTAGCTTTTGAATTGCTCAGTTGAACTTGTATGGTTTCAGAATTTGCAACAAGATATTACACTTCAAAAATTATTGTAGCTGATATAGTGAATGTTGGCGTAATTGTGAGTAATCAAATGGCCATTTGAGCTCTAAGGTAATAGCCTTTTCGTGTGAATGCACTTAAATGGGTTTTGTTTTCGCAGGAAGACTTCGAGAAATTTTGGTCAAAATGTTCATGGGACGATGATTTGAAGTATGCCAAAGCTGTTTGTGATCATGTTTGTTACTATTTTAGCTTATAAATTCATCTAGACTTACAAAGGTTAATTTTTCCAATGAATATTCAAATGAAATAAAGGATTTGTTGGGAAAATTAAACATAATTCAAGATACCCACAGGTAATGTCTTTCTTCTGCAGTTAGTATTGTCTTTCTTTTGTTGGAAAAATTAAACACTATTTTGCTATTTTCATGACTTTTGTCTATATTCTACACTACTCATGGTAGTTAGTAAGGCCTTTAGTAATTTAACTTGAAGTTTCATGTATAAGCTCAGTGGAAATAGATATGACTAATATTGTATTATACGGCCAATATTTGTTGCAATCCTTTAGGAAACAACATCATTAATAAGCTTTATGATTTATATCACTTATCTATTCAAGGTTTATCATCAAACACATTTATTCCTTCCTTGTTCCTTCTTGCTCCAAAATAAAGATTTCTTATGTTACTTGAATATTATTGTTCTTAGGTCAAGGATCAAACATATTTTCTATCACATCTCTCACAGAATCAACTTAAAAGGCTTTTATTCCCTCTTGGTTGTATTCCAAAGGTTTGTTCAAGCTAAATTTATAGTCTTTATTATCATGTATTTTTTTTTTTGAGGTATCTCTTATGTTTCAGACTTCAGGGTGTATTCCGAGAAGTTCAAGTTCAACTGTTGTAAATTTGTAAGATTTGGTACTAGCCCTCTAACCTTGTACAGACATAAACACCTAGTAGCTAACATTGATTTCATTAAGTTTAGAAATTTATGTATTTAATAAAAGTTAAAAATTGTATTATAGATTTTGGGAAGCCAATTAATGTAGATAACATTTTAGAGATTTGTGGATGTTATATCGCAATTTAAACCTATTAAGTTTTCACTATTCTCGTGTGCAAAACTTATATAAGTGCCTCAATTTATTAGTTCATATCATGCTAACTCCTATCCCAACTAGCTACATTTTTTCAACTTATGCTCTATCTTTTCGGAAGGAAAAAGGAATACTTGTGTGATTGTTATCTTGTTAATATGTTTTTCTAGGATTGTGACTTTAGCAATATTTTTCTTCTCTCAACTTTATATTCCTTCAACAATCGGTTTTAAACCGACATTAAAGGCCAAATTTCTTGTAGTGTTGATAGATCTATCGGTGTGA

mRNA sequence

ATGTCCTCCATGTTTTATTCGAGCCAATGTGTAGAAAATGGCAAGGCCTCTTTTGGGGTTGTTGAAGCCGTTAACCTTATAACTTCTTTGAAATTATTTGGTTTACTCTTGGTTGTTGGCTATCCGGAAGACTTCGAGAAATTTTGGTCAAAATGTTCATGGGACGATGATTTGAAGTATGCCAAAGCTGTTTGTGATCATGTCAAGGATCAAACATATTTTCTATCACATCTCTCACAGAATCAACTTAAAAGGCTTTTATTCCCTCTTGGTTGTATTCCAAAGTTGAGGTATCTCTTATGTTTCAGACTTCAGGGTGTATTCCGAGAAGTTCAAGTTCAACTGTTGATTGTGACTTTAGCAATATTTTTCTTCTCTCAACTTTATATTCCTTCAACAATCGGTTTTAAACCGACATTAAAGGCCAAATTTCTTGTAGTGTTGATAGATCTATCGGTGTGA

Coding sequence (CDS)

ATGTCCTCCATGTTTTATTCGAGCCAATGTGTAGAAAATGGCAAGGCCTCTTTTGGGGTTGTTGAAGCCGTTAACCTTATAACTTCTTTGAAATTATTTGGTTTACTCTTGGTTGTTGGCTATCCGGAAGACTTCGAGAAATTTTGGTCAAAATGTTCATGGGACGATGATTTGAAGTATGCCAAAGCTGTTTGTGATCATGTCAAGGATCAAACATATTTTCTATCACATCTCTCACAGAATCAACTTAAAAGGCTTTTATTCCCTCTTGGTTGTATTCCAAAGTTGAGGTATCTCTTATGTTTCAGACTTCAGGGTGTATTCCGAGAAGTTCAAGTTCAACTGTTGATTGTGACTTTAGCAATATTTTTCTTCTCTCAACTTTATATTCCTTCAACAATCGGTTTTAAACCGACATTAAAGGCCAAATTTCTTGTAGTGTTGATAGATCTATCGGTGTGA

Protein sequence

MSSMFYSSQCVENGKASFGVVEAVNLITSLKLFGLLLVVGYPEDFEKFWSKCSWDDDLKYAKAVCDHVKDQTYFLSHLSQNQLKRLLFPLGCIPKLRYLLCFRLQGVFREVQVQLLIVTLAIFFFSQLYIPSTIGFKPTLKAKFLVVLIDLSV
Homology
[Close]
BLAST of MELO3C035704 vs. ExPASy Swiss-Prot
Match: Q0SMH1 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN=mnmA PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 7.7e-05
Identity = 22/47 (46.81%), Postives = 30/47 (63.83%), Query Frame = 0 

Query: 49  WSKCSWDDDLKYAKAVCDHVKDQTYFLSHLSQNQLKRLLFPLGCIPK 96
           ++K    D   + K   D +KDQ+YFLSHLSQ Q+ +L FPLG + K
Sbjct: 125 YAKIQTKDKKFFLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLK 171

[Close]
BLAST of MELO3C035704 vs. ExPASy Swiss-Prot
Match: Q1LJ45 (tRNA-specific 2-thiouridylase MnmA OS=Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34) OX=266264 GN=mnmA PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.7e-04
Identity = 22/32 (68.75%), Postives = 24/32 (75.00%), Query Frame = 0 

Query: 66  DHVKDQTYFLSHLSQNQLKRLLFPLGCIPKLR 98
           DH KDQ+YFL  L+Q QL R LFPLG IPK R
Sbjct: 144 DHTKDQSYFLHRLNQAQLSRTLFPLGEIPKTR 175

[Close]
BLAST of MELO3C035704 vs. ExPASy Swiss-Prot
Match: Q0K720 (tRNA-specific 2-thiouridylase MnmA OS=Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) OX=381666 GN=mnmA PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.7e-04
Identity = 22/32 (68.75%), Postives = 24/32 (75.00%), Query Frame = 0 

Query: 66  DHVKDQTYFLSHLSQNQLKRLLFPLGCIPKLR 98
           DH KDQ+YFL  L+Q QL R LFPLG IPK R
Sbjct: 146 DHTKDQSYFLHRLNQAQLSRTLFPLGEIPKTR 177

[Close]
BLAST of MELO3C035704 vs. ExPASy Swiss-Prot
Match: Q46XF1 (tRNA-specific 2-thiouridylase MnmA OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) OX=264198 GN=mnmA PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.7e-04
Identity = 22/32 (68.75%), Postives = 24/32 (75.00%), Query Frame = 0 

Query: 66  DHVKDQTYFLSHLSQNQLKRLLFPLGCIPKLR 98
           DH KDQ+YFL  L+Q QL R LFPLG IPK R
Sbjct: 144 DHTKDQSYFLHRLNQAQLSRTLFPLGEIPKTR 175

[Close]
BLAST of MELO3C035704 vs. ExPASy Swiss-Prot
Match: B3R6Q0 (tRNA-specific 2-thiouridylase MnmA OS=Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CCUG 44338 / CIP 107171 / LMG 19424 / R1) OX=977880 GN=mnmA PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.7e-04
Identity = 22/32 (68.75%), Postives = 24/32 (75.00%), Query Frame = 0 

Query: 66  DHVKDQTYFLSHLSQNQLKRLLFPLGCIPKLR 98
           DH KDQ+YFL  L+Q QL R LFPLG IPK R
Sbjct: 146 DHTKDQSYFLHRLNQAQLSRTLFPLGEIPKTR 177

[Close]
BLAST of MELO3C035704 vs. NCBI nr
Match: KAE9463251.1 (hypothetical protein C3L33_04838, partial [Rhododendron williamsianum])

HSP 1 Score: 94.7 bits (234), Expect = 7.2e-16
Identity = 44/74 (59.46%), Postives = 52/74 (70.27%), Query Frame = 0 

Query: 43 EDFEKFWSKCSWDDDLKYAKAVCDH------------------VKDQTYFLSHLSQNQLK 99
          EDFE FWS+C W++DLKYAK VC+                   VKDQTYFLSHLSQ+QLK
Sbjct: 14 EDFENFWSECPWEEDLKYAKVVCNQVDVPLEVXHLTDEYWNNVVKDQTYFLSHLSQSQLK 73

[Close]
BLAST of MELO3C035704 vs. NCBI nr
Match: RWW67338.1 (hypothetical protein BHE74_00025214 [Ensete ventricosum])

HSP 1 Score: 92.0 bits (227), Expect = 4.6e-15
Identity = 45/89 (50.56%), Postives = 53/89 (59.55%), Query Frame = 0 

Query: 42  PEDFEKFWSKCSWDDDLKYAKAVCDHV--------------------------------K 99
           PEDFE FWS+C WD+DLKYA+AVCD V                                K
Sbjct: 199 PEDFENFWSECPWDEDLKYARAVCDQVRCFLLWDFCLSMNVDVPLEVVHLSDEYWNNVIK 258

[Close]
BLAST of MELO3C035704 vs. NCBI nr
Match: RRT63170.1 (hypothetical protein B296_00042876 [Ensete ventricosum])

HSP 1 Score: 92.0 bits (227), Expect = 4.6e-15
Identity = 45/89 (50.56%), Postives = 53/89 (59.55%), Query Frame = 0 

Query: 42  PEDFEKFWSKCSWDDDLKYAKAVCDHV--------------------------------K 99
           PEDFE FWS+C WD+DLKYA+AVCD V                                K
Sbjct: 199 PEDFENFWSECPWDEDLKYARAVCDQVRCFLLWDFCLSMNVDVPLEVVHLSDEYWNNVIK 258

[Close]
BLAST of MELO3C035704 vs. NCBI nr
Match: RWW14291.1 (hypothetical protein GW17_00021947 [Ensete ventricosum] >RZS08586.1 hypothetical protein BHM03_00039571 [Ensete ventricosum])

HSP 1 Score: 92.0 bits (227), Expect = 4.6e-15
Identity = 45/89 (50.56%), Postives = 53/89 (59.55%), Query Frame = 0 

Query: 42  PEDFEKFWSKCSWDDDLKYAKAVCDHV--------------------------------K 99
           PEDFE FWS+C WD+DLKYA+AVCD V                                K
Sbjct: 197 PEDFENFWSECPWDEDLKYARAVCDQVRCFLLWDFCLSMNVDVPLEVVHLSDEYWNNVIK 256

[Close]
BLAST of MELO3C035704 vs. NCBI nr
Match: GFP98782.1 (tRNA-specific 2-thiouridylase mnma [Phtheirospermum japonicum])

HSP 1 Score: 80.1 bits (196), Expect = 1.8e-11
Identity = 44/108 (40.74%), Postives = 51/108 (47.22%), Query Frame = 0 

Query: 37  LVVGYPEDFEKFWSKCSWDDDLKYAKAVCDH----------------------------- 96
           L + + EDFE FWS+C W++DLKYAKAVCD                              
Sbjct: 123 LKIWFQEDFENFWSECPWEEDLKYAKAVCDQIDVPLEGAFVDAVRGMKFDFIASGHYAKV 182

[Close]
BLAST of MELO3C035704 vs. ExPASy TrEMBL
Match: A0A6A4M3X7 (Uncharacterized protein (Fragment) OS=Rhododendron williamsianum OX=262921 GN=C3L33_04838 PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 3.5e-16
Identity = 44/74 (59.46%), Postives = 52/74 (70.27%), Query Frame = 0 

Query: 43 EDFEKFWSKCSWDDDLKYAKAVCDH------------------VKDQTYFLSHLSQNQLK 99
          EDFE FWS+C W++DLKYAK VC+                   VKDQTYFLSHLSQ+QLK
Sbjct: 14 EDFENFWSECPWEEDLKYAKVVCNQVDVPLEVXHLTDEYWNNVVKDQTYFLSHLSQSQLK 73

[Close]
BLAST of MELO3C035704 vs. ExPASy TrEMBL
Match: A0A444EVC8 (Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=GW17_00021947 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.2e-15
Identity = 45/89 (50.56%), Postives = 53/89 (59.55%), Query Frame = 0 

Query: 42  PEDFEKFWSKCSWDDDLKYAKAVCDHV--------------------------------K 99
           PEDFE FWS+C WD+DLKYA+AVCD V                                K
Sbjct: 197 PEDFENFWSECPWDEDLKYARAVCDQVRCFLLWDFCLSMNVDVPLEVVHLSDEYWNNVIK 256

[Close]
BLAST of MELO3C035704 vs. ExPASy TrEMBL
Match: A0A426ZGQ9 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Ensete ventricosum OX=4639 GN=B296_00042876 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 2.2e-15
Identity = 45/89 (50.56%), Postives = 53/89 (59.55%), Query Frame = 0 

Query: 42  PEDFEKFWSKCSWDDDLKYAKAVCDHV--------------------------------K 99
           PEDFE FWS+C WD+DLKYA+AVCD V                                K
Sbjct: 199 PEDFENFWSECPWDEDLKYARAVCDQVRCFLLWDFCLSMNVDVPLEVVHLSDEYWNNVIK 258

[Close]
BLAST of MELO3C035704 vs. ExPASy TrEMBL
Match: K7LPK6 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Glycine max OX=3847 GN=100785275 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 4.2e-14
Identity = 42/70 (60.00%), Postives = 48/70 (68.57%), Query Frame = 0 

Query: 44 DFEKFWSKCSWDDDLKYAKAVCDH------------------VKDQTYFLSHLSQNQLKR 96
          +FE FWS+C W+DDLKYAK VC+                   VKDQTYFLSHLSQ+QLK+
Sbjct: 4  NFENFWSECPWEDDLKYAKDVCNQVDVPLEVVHLTDEYWNNVVKDQTYFLSHLSQSQLKQ 63

[Close]
BLAST of MELO3C035704 vs. ExPASy TrEMBL
Match: A0A0D3FEH8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Oryza barthii OX=65489 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.0e-12
Identity = 45/107 (42.06%), Postives = 50/107 (46.73%), Query Frame = 0 

Query: 37  LVVGYPEDFEKFWSKCSWDDDLKYAKAVC------------------------------- 96
           L + + EDF  FWS+C WDDDLKYA+AVC                               
Sbjct: 109 LKIWFQEDFRNFWSECPWDDDLKYAQAVCDKSIYLMRAFLEAIENLGFDYIASGHYAHVV 168

[Close]
BLAST of MELO3C035704 vs. TAIR 10
Match: AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases )

HSP 1 Score: 62.8 bits (151), Expect = 2.8e-10
Identity = 43/146 (29.45%), Postives = 49/146 (33.56%), Query Frame = 0 

Query: 37  LVVGYPEDFEKFWSKCSWDDDLKYAKAVC------------------------------- 96
           L + + E FE FW++C W+DDLKYAK VC                               
Sbjct: 113 LKIWFQEGFENFWNQCPWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCG 172

The following BLAST results are available for this feature:
BLAST of MELO3C035704 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q0SMH17.7e-0546.81tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN... [more]
Q1LJ451.7e-0468.75tRNA-specific 2-thiouridylase MnmA OS=Cupriavidus metallidurans (strain ATCC 431... [more]
Q0K7201.7e-0468.75tRNA-specific 2-thiouridylase MnmA OS=Cupriavidus necator (strain ATCC 17699 / D... [more]
Q46XF11.7e-0468.75tRNA-specific 2-thiouridylase MnmA OS=Cupriavidus pinatubonensis (strain JMP 134... [more]
B3R6Q01.7e-0468.75tRNA-specific 2-thiouridylase MnmA OS=Cupriavidus taiwanensis (strain DSM 17343 ... [more]
BLAST of MELO3C035704 vs. NCBI nr
Match NameE-valueIdentityDescription
KAE9463251.17.2e-1659.46hypothetical protein C3L33_04838, partial [Rhododendron williamsianum][more]
RWW67338.14.6e-1550.56hypothetical protein BHE74_00025214 [Ensete ventricosum][more]
RRT63170.14.6e-1550.56hypothetical protein B296_00042876 [Ensete ventricosum][more]
RWW14291.14.6e-1550.56hypothetical protein GW17_00021947 [Ensete ventricosum] >RZS08586.1 hypothetical... [more]
GFP98782.11.8e-1140.74tRNA-specific 2-thiouridylase mnma [Phtheirospermum japonicum][more]
BLAST of MELO3C035704 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6A4M3X73.5e-1659.46Uncharacterized protein (Fragment) OS=Rhododendron williamsianum OX=262921 GN=C3... [more]
A0A444EVC82.2e-1550.56Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=GW17_00021947 PE=4 SV=1[more]
A0A426ZGQ92.2e-1550.56tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Ensete ventricosum OX=4639 GN... [more]
K7LPK64.2e-1460.00tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Glycine max OX=3847 GN=100785... [more]
A0A0D3FEH83.0e-1242.06tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Oryza barthii OX=65489 PE=3 S... [more]
BLAST of MELO3C035704 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G51310.12.8e-1029.45transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 66..98
e-value: 7.9E-8
score: 33.9
NoneNo IPR availablePFAMPF03054tRNA_Me_transcoord: 66..95
e-value: 8.8E-8
score: 31.3
NoneNo IPR availablePANTHERPTHR43052FAMILY NOT NAMEDcoord: 39..69
coord: 66..95

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C035704.1MELO3C035704.1mRNA