MELO3C034352 (gene) Melon (DHL92) v4

Overview
NameMELO3C034352
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionCystatin domain-containing protein
Locationchr10: 11504987 .. 11505307 (+)
RNA-Seq ExpressionMELO3C034352
SyntenyMELO3C034352
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTGTGTAGAAGTACCTCCAAATACTTATGACCCGATTGTGGATGTCAATAGTCCAACTGTGGTAGAAGCTGGAAAGATGGCAGTGGAAGAACATAACAAAAAAGAAAATGACAATTTAGTTTTCGTTCGTGTAGTGAGTGGTTTAACACCACGTTTTATTGTTGGGATTTTATTATGGTCATATATTATTGTGATAGAAGCTAAAACTTGCTATGGATATCCCGTGGCATATGTGGCTGAAATAGTCAAGTATGTTTCATTTCCACCCGATAGCTATAAACTTATCAGTTTTAAACCTATACTCAAGTATGAGTAA

mRNA sequence

ATGAGTTGTGTAGAAGTACCTCCAAATACTTATGACCCGATTGTGGATGTCAATAGTCCAACTGTGGTAGAAGCTGGAAAGATGGCAGTGGAAGAACATAACAAAAAAGAAAATGACAATTTAGTTTTCGTTCGTGTAGTGAGTGGTTTAACACCACGTTTTATTGTTGGGATTTTATTATGGTCATATATTATTGTGATAGAAGCTAAAACTTGCTATGGATATCCCGTGGCATATGTGGCTGAAATAGTCAAGTATGTTTCATTTCCACCCGATAGCTATAAACTTATCAGTTTTAAACCTATACTCAAGTATGAGTAA

Coding sequence (CDS)

ATGAGTTGTGTAGAAGTACCTCCAAATACTTATGACCCGATTGTGGATGTCAATAGTCCAACTGTGGTAGAAGCTGGAAAGATGGCAGTGGAAGAACATAACAAAAAAGAAAATGACAATTTAGTTTTCGTTCGTGTAGTGAGTGGTTTAACACCACGTTTTATTGTTGGGATTTTATTATGGTCATATATTATTGTGATAGAAGCTAAAACTTGCTATGGATATCCCGTGGCATATGTGGCTGAAATAGTCAAGTATGTTTCATTTCCACCCGATAGCTATAAACTTATCAGTTTTAAACCTATACTCAAGTATGAGTAA

Protein sequence

MSCVEVPPNTYDPIVDVNSPTVVEAGKMAVEEHNKKENDNLVFVRVVSGLTPRFIVGILLWSYIIVIEAKTCYGYPVAYVAEIVKYVSFPPDSYKLISFKPILKYE
Homology
BLAST of MELO3C034352 vs. NCBI nr
Match: KAE8648095.1 (hypothetical protein Csa_004720 [Cucumis sativus])

HSP 1 Score: 155.2 bits (391), Expect = 3.1e-34
Identity = 75/106 (70.75%), Postives = 92/106 (86.79%), Query Frame = 0

Query: 1   MSCVEVPPNTYDPIVDVNSPTVVEAGKMAVEEHNKKENDNLVFVRVVSGLTPRFIVGILL 60
           MSCV+V PNT+D I DV+SP V+EAGK+AV+EHNKKENDNLVF RVVSGL PR I+G +L
Sbjct: 1   MSCVQVLPNTHDLIKDVHSPNVIEAGKIAVDEHNKKENDNLVFTRVVSGLAPRAIIGTIL 60

Query: 61  WSYIIVIEAKTCYGYPVAYVAEIVKYVSFPPDSYKLISFKPILKYE 107
           WSYI+VIEAK C GYPVAYVA+IVKY++ P +SY+L+SF+ IL Y+
Sbjct: 61  WSYILVIEAKNCDGYPVAYVAKIVKYLTIPENSYELVSFQAILGYK 106

BLAST of MELO3C034352 vs. NCBI nr
Match: KAE8648094.1 (hypothetical protein Csa_004738 [Cucumis sativus])

HSP 1 Score: 150.6 bits (379), Expect = 7.6e-33
Identity = 72/106 (67.92%), Postives = 90/106 (84.91%), Query Frame = 0

Query: 1   MSCVEVPPNTYDPIVDVNSPTVVEAGKMAVEEHNKKENDNLVFVRVVSGLTPRFIVGILL 60
           MSCV+VPPNT+D I DV+SP VVEAGK+AVEEHNKKENDNLVF+RVVS + PR ++ I L
Sbjct: 1   MSCVQVPPNTHDLIKDVHSPDVVEAGKVAVEEHNKKENDNLVFIRVVSAIAPRALIFIKL 60

Query: 61  WSYIIVIEAKTCYGYPVAYVAEIVKYVSFPPDSYKLISFKPILKYE 107
           WS+I+VIEAKTC G+PV YV EI KY++ P +SYKLI F+ +L+Y+
Sbjct: 61  WSFILVIEAKTCNGFPVTYVVEIDKYLTIPEESYKLIRFEAVLEYK 106

BLAST of MELO3C034352 vs. NCBI nr
Match: VFQ87820.1 (unnamed protein product [Cuscuta campestris])

HSP 1 Score: 60.5 bits (145), Expect = 1.0e-05
Identity = 40/88 (45.45%), Postives = 51/88 (57.95%), Query Frame = 0

Query: 15  VDVNSPTVVEAGKMAVEEHNKKENDNLVFVRVVSGLTPRFIVGILLWSYIIVIEAKTCYG 74
           VD  SP VV AG  AV+ HNK ENDNL F  V SG   + + G   + Y +VI+A++ +G
Sbjct: 36  VDPESPEVVAAGNFAVDAHNKAENDNLGFEDVASG-EEQVVAG---FQYKLVIDARSGHG 95

Query: 75  YPVAYVAEIVKYVSFPPDSYKLISFKPI 103
               Y  E V +    PDSY LISFK +
Sbjct: 96  ---VYQYEAVVWAKPSPDSYSLISFKQL 116

BLAST of MELO3C034352 vs. NCBI nr
Match: KAE8649716.1 (hypothetical protein Csa_012476 [Cucumis sativus])

HSP 1 Score: 55.5 bits (132), Expect = 3.3e-04
Identity = 37/103 (35.92%), Postives = 56/103 (54.37%), Query Frame = 0

Query: 11  YDPIVDVNSPTVVEAGKMAVEEHNKKENDNLVFVRVVSGLTPRFI-----VGILLWSYII 70
           ++PI D+N P V E G + V++HNKK  +NL FVRVV+GL   F+     +G  +  Y +
Sbjct: 7   FEPIKDINDPQVQEIGNLTVDDHNKKTGENLKFVRVVNGLFAGFVDPFSPLGGTV--YEL 66

Query: 71  VIEAKTCYGYPVAYVAEIVKYVSFPPDS--YKLISFKPILKYE 107
           V+EA         Y  ++    +  P    Y   SF+P+L+YE
Sbjct: 67  VLEAINAGKINWTYKVKVSASTTIRPLGVVYNFQSFEPVLQYE 107

BLAST of MELO3C034352 vs. NCBI nr
Match: XP_008442277.1 (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa] >TYK05422.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa])

HSP 1 Score: 55.1 bits (131), Expect = 4.4e-04
Identity = 40/108 (37.04%), Postives = 58/108 (53.70%), Query Frame = 0

Query: 9   NTYDPIVDVN-SPTVVEAGKMAVEEHNKKENDNLVFVRVVSGLTPRF------IVGILLW 68
           + + PI D+N +  V + G + V++HNKK  +NL FVRVV+GL   F      + G+L  
Sbjct: 5   SAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGGVL-- 64

Query: 69  SYIIVIEAKTCYGYPVAYVAEIVKYVSFPPD---SYKLISFKPILKYE 107
            Y +V+EA T       Y A + K  +  P     Y   SFKP+L+YE
Sbjct: 65  -YELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYE 109

BLAST of MELO3C034352 vs. ExPASy TrEMBL
Match: A0A484MG82 (Cystatin domain-containing protein OS=Cuscuta campestris OX=132261 GN=CCAM_LOCUS29596 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 5.0e-06
Identity = 40/88 (45.45%), Postives = 51/88 (57.95%), Query Frame = 0

Query: 15  VDVNSPTVVEAGKMAVEEHNKKENDNLVFVRVVSGLTPRFIVGILLWSYIIVIEAKTCYG 74
           VD  SP VV AG  AV+ HNK ENDNL F  V SG   + + G   + Y +VI+A++ +G
Sbjct: 36  VDPESPEVVAAGNFAVDAHNKAENDNLGFEDVASG-EEQVVAG---FQYKLVIDARSGHG 95

Query: 75  YPVAYVAEIVKYVSFPPDSYKLISFKPI 103
               Y  E V +    PDSY LISFK +
Sbjct: 96  ---VYQYEAVVWAKPSPDSYSLISFKQL 116

BLAST of MELO3C034352 vs. ExPASy TrEMBL
Match: A0A0A0L3W1 (Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE=4 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 5.6e-05
Identity = 43/111 (38.74%), Postives = 61/111 (54.95%), Query Frame = 0

Query: 4   VEVPPNTYDPIVDVNSPTVVEA--GKMAVEEHNKKENDNLVFVRVVSGLTPRFIV----- 63
           VE   +T  PI D+N    V++  G++AVE+HNKK  DNL  V VV+GL     V     
Sbjct: 12  VEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPGST 71

Query: 64  --GILLWSYIIVIEAKTCYGYPVAYVAEIVKYVSFPPDSYKLISFKPILKY 106
             GIL   Y +++EAKT  G    YVA++++        Y+  SF+P+L Y
Sbjct: 72  HEGIL---YHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPLLPY 119

BLAST of MELO3C034352 vs. ExPASy TrEMBL
Match: A0A5A7TJK0 (Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001140 PE=4 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.1e-04
Identity = 40/108 (37.04%), Postives = 58/108 (53.70%), Query Frame = 0

Query: 9   NTYDPIVDVN-SPTVVEAGKMAVEEHNKKENDNLVFVRVVSGLTPRF------IVGILLW 68
           + + PI D+N +  V + G + V++HNKK  +NL FVRVV+GL   F      + G+L  
Sbjct: 5   SAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGGVL-- 64

Query: 69  SYIIVIEAKTCYGYPVAYVAEIVKYVSFPPD---SYKLISFKPILKYE 107
            Y +V+EA T       Y A + K  +  P     Y   SFKP+L+YE
Sbjct: 65  -YELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYE 109

BLAST of MELO3C034352 vs. ExPASy TrEMBL
Match: A0A484KUC3 (Cystatin domain-containing protein OS=Cuscuta campestris OX=132261 GN=CCAM_LOCUS9871 PE=4 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.1e-04
Identity = 43/100 (43.00%), Postives = 55/100 (55.00%), Query Frame = 0

Query: 7   PPNTYDPIVDVNSPTVVEAGKMAVEEHNKKENDNLVFVRVVSG---LTPRFIVGILLWSY 66
           P   ++P VD  SP VV AG  AVE HN+ ENDNLVF  V SG   +  RF        Y
Sbjct: 28  PAGGWEP-VDPKSPEVVAAGSFAVEAHNEAENDNLVFEDVASGEKQMAARF-------QY 87

Query: 67  IIVIEAKTCYGYPVAYVAEIVKYVSFPP-DSYKLISFKPI 103
            +VI+A++ +G    Y  E V +V     +SY LISFK +
Sbjct: 88  KLVIDARSGHG---VYQYEAVVWVKRSRLNSYSLISFKQL 116

BLAST of MELO3C034352 vs. ExPASy TrEMBL
Match: A0A1S3B5B9 (cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=4 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.1e-04
Identity = 40/108 (37.04%), Postives = 58/108 (53.70%), Query Frame = 0

Query: 9   NTYDPIVDVN-SPTVVEAGKMAVEEHNKKENDNLVFVRVVSGLTPRF------IVGILLW 68
           + + PI D+N +  V + G + V++HNKK  +NL FVRVV+GL   F      + G+L  
Sbjct: 5   SAFKPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGGVL-- 64

Query: 69  SYIIVIEAKTCYGYPVAYVAEIVKYVSFPPD---SYKLISFKPILKYE 107
            Y +V+EA T       Y A + K  +  P     Y   SFKP+L+YE
Sbjct: 65  -YELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYE 109

BLAST of MELO3C034352 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 43.9 bits (102), Expect = 9.4e-05
Identity = 35/90 (38.89%), Postives = 48/90 (53.33%), Query Frame = 0

Query: 13  PIVDVNSPTVVEAGKMAVEEHNKKENDNLVFVRVVSGLTPRFIVGILLWSYIIVIEAKTC 72
           PI +V+ P VV   K A+EEHNK+  + LVFV+VV G T + + G     Y + I AK  
Sbjct: 34  PIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEG-TTQVVSGT---KYDLKIAAKDG 93

Query: 73  YGYPVAYVAEIVKYVSFPPDSYKLISFKPI 103
            G    Y A +V+ +     S  L SFK +
Sbjct: 94  GGKIKNYEAVVVEKLWL--HSKSLESFKAL 117

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8648095.13.1e-3470.75hypothetical protein Csa_004720 [Cucumis sativus][more]
KAE8648094.17.6e-3367.92hypothetical protein Csa_004738 [Cucumis sativus][more]
VFQ87820.11.0e-0545.45unnamed protein product [Cuscuta campestris][more]
KAE8649716.13.3e-0435.92hypothetical protein Csa_012476 [Cucumis sativus][more]
XP_008442277.14.4e-0437.04PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cys... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A484MG825.0e-0645.45Cystatin domain-containing protein OS=Cuscuta campestris OX=132261 GN=CCAM_LOCUS... [more]
A0A0A0L3W15.6e-0538.74Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE... [more]
A0A5A7TJK02.1e-0437.04Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A484KUC32.1e-0443.00Cystatin domain-containing protein OS=Cuscuta campestris OX=132261 GN=CCAM_LOCUS... [more]
A0A1S3B5B92.1e-0437.04cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=... [more]
Match NameE-valueIdentityDescription
AT4G16500.19.4e-0538.89Cystatin/monellin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.450.10coord: 10..106
e-value: 7.6E-13
score: 50.3
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 18..103
IPR000010Cystatin domainPFAMPF16845SQAPIcoord: 14..101
e-value: 3.0E-11
score: 43.5
IPR000010Cystatin domainCDDcd00042CYcoord: 15..72
e-value: 7.12128E-6
score: 39.3796

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C034352.1MELO3C034352.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity