MELO3C031025 (gene) Melon (DHL92) v4

Overview


Name	MELO3C031025
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	Ribonuclease T(2)
Location	chr04: 31812932 .. 31814155 (-)
RNA-Seq Expression	MELO3C031025
Synteny	MELO3C031025

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCAGCTCTCATTATTCTACATTTCCATACTCAGCTTATTGTTCATTTCTCAATGGCTCAGCATACTTTCATTTTATGTCTTTTAAGTTTGTTGTTGTTTGTGAATGGCTATCCATTCGACTTCTTCCAACTAGTCGAACAATGGGGTCCAAACACATGTAACGACGGCACCCAGTGTCATGCTCAACCTCAACCCGAATTCACTATTCATGGGCTGTGGCCGAGTAACTTTTCAAATGCCCGACTTTTTTGTGCAACTAGAAAACTATTTGACAATGGCCAGGTTCCCATCTTCTTTATATATATATATATATACATATATATGTTGTGTGTGTGTGCGCTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTATCTTTAGCATTCTATTCATTGTTTCAACACATTCTTTTTATTTTATTTTAGATTAGCTTACTTGAAGGAGACCTAGATCGTTACTGGCCGGATGTAATAAATGGGAACAACCGCGGCTTCTGGAGTAATGAATGGAAGAAGCACGGGGCATGTATAGACCCACCTTTTAACATAACTCAGTATTTCCAACTTTCTTTGGATCATCATCGAACGTATGATCTGTTGAAAATCTTAAATGGTGTTTGGCACCGCAACCGCCAAATGCCCAATAATGGTCTCGCACCTAAAGACATTCTAAACCCCATTTTTAATGCAACAAACAAGACAGCAGGCATCCGTTGCAACAAAAATGGTATAACTAAGAAGTTGCAATTGCATGAAATTGTACTGTGTTTTGACAACAATGGTACTACCCTCATTGATTGCCCTAAGTTTGTTAGCAACAGTTGTAATCAACCTAAATTTGTGTGGCTCCTCCCACAACAGAGTTCAGATGGTGTTCCAGATTATGCATGATCATTTGATATCCTCATCGTTTGTTATGCTTAGAAGACCTATTAATTACAGGAAGTTTATGTTTTTCTCCTTATTAAATCAGAATGTGTGTTCACTGTTGTCGCTTAATGTTTAAGTAAGTGTTTGTGTTGGTTGTCGTCGTTAAGTATGTGTTTAAATGGATCAGTTATTGATGTTTATGTGTGTTCAGCCTGTTGTCATTAAGCAATGTTTAAATCATTTTAATTATGTGTTCACTATTGTTTATTGCTAAATCAGAAAAATTCATCACCAATGTCCCACATTTGTAAGGTCAAA

mRNA sequence

TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCAGCTCTCATTATTCTACATTTCCATACTCAGCTTATTGTTCATTTCTCAATGGCTCAGCATACTTTCATTTTATGTCTTTTAAGTTTGTTGTTGTTTGTGAATGGCTATCCATTCGACTTCTTCCAACTAGTCGAACAATGGGGTCCAAACACATGTAACGACGGCACCCAGTGTCATGCTCAACCTCAACCCGAATTCACTATTCATGGGCTGTGGCCGAGTAACTTTTCAAATGCCCGACTTTTTTGTGCAACTAGAAAACTATTTGACAATGGCCAGATTAGCTTACTTGAAGGAGACCTAGATCGTTACTGGCCGGATGTAATAAATGGGAACAACCGCGGCTTCTGGAGTAATGAATGGAAGAAGCACGGGGCATGTATAGACCCACCTTTTAACATAACTCAGTATTTCCAACTTTCTTTGGATCATCATCGAACGTATGATCTGTTGAAAATCTTAAATGGTGTTTGGCACCGCAACCGCCAAATGCCCAATAATGGTCTCGCACCTAAAGACATTCTAAACCCCATTTTTAATGCAACAAACAAGACAGCAGGCATCCGTTGCAACAAAAATGGTATAACTAAGAAGTTGCAATTGCATGAAATTGTACTGTGTTTTGACAACAATGGTACTACCCTCATTGATTGCCCTAAGTTTGTTAGCAACAGTTGTAATCAACCTAAATTTGTGTGGCTCCTCCCACAACAGAGTTCAGATGGTGTTCCAGATTATGCATGATCATTTGATATCCTCATCGTTTGTTATGCTTAGAAGACCTATTAATTACAGGAAGTTTATGTTTTTCTCCTTATTAAATCAGAATGTGTGTTCACTGTTGTCGCTTAATGTTTAAGTAAGTGTTTGTGTTGGTTGTCGTCGTTAAGTATGTGTTTAAATGGATCAGTTATTGATGTTTATGTGTGTTCAGCCTGTTGTCATTAAGCAATGTTTAAATCATTTTAATTATGTGTTCACTATTGTTTATTGCTAAATCAGAAAAATTCATCACCAATGTCCCACATTTGTAAGGTCAAA

Coding sequence (CDS)

ATGGCTCAGCATACTTTCATTTTATGTCTTTTAAGTTTGTTGTTGTTTGTGAATGGCTATCCATTCGACTTCTTCCAACTAGTCGAACAATGGGGTCCAAACACATGTAACGACGGCACCCAGTGTCATGCTCAACCTCAACCCGAATTCACTATTCATGGGCTGTGGCCGAGTAACTTTTCAAATGCCCGACTTTTTTGTGCAACTAGAAAACTATTTGACAATGGCCAGATTAGCTTACTTGAAGGAGACCTAGATCGTTACTGGCCGGATGTAATAAATGGGAACAACCGCGGCTTCTGGAGTAATGAATGGAAGAAGCACGGGGCATGTATAGACCCACCTTTTAACATAACTCAGTATTTCCAACTTTCTTTGGATCATCATCGAACGTATGATCTGTTGAAAATCTTAAATGGTGTTTGGCACCGCAACCGCCAAATGCCCAATAATGGTCTCGCACCTAAAGACATTCTAAACCCCATTTTTAATGCAACAAACAAGACAGCAGGCATCCGTTGCAACAAAAATGGTATAACTAAGAAGTTGCAATTGCATGAAATTGTACTGTGTTTTGACAACAATGGTACTACCCTCATTGATTGCCCTAAGTTTGTTAGCAACAGTTGTAATCAACCTAAATTTGTGTGGCTCCTCCCACAACAGAGTTCAGATGGTGTTCCAGATTATGCATGA

Protein sequence

MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCNDGTQCHAQPQPEFTIHGLWPSNFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNITQYFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNKNGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDYA

Homology

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BLAST of MELO3C031025 vs. NCBI nr
Match: TYK25090.1 (S-RNase [Cucumis melo var. makuwa])

HSP 1 Score: 511.9 bits (1317), Expect = 2.8e-141
Identity = 231/231 (100.00%), Postives = 231/231 (100.00%), Query Frame = 0


Query: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCNDGTQCHAQPQPEFTIHGLWPSNF 60
           MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCNDGTQCHAQPQPEFTIHGLWPSNF
Sbjct: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCNDGTQCHAQPQPEFTIHGLWPSNF 60

Query: 61  SNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNITQ 120
           SNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNITQ
Sbjct: 61  SNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNITQ 120

Query: 121 YFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNKNGIT 180
           YFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNKNGIT
Sbjct: 121 YFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNKNGIT 180

Query: 181 KKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDYA 232
           KKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDYA
Sbjct: 181 KKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDYA 231

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BLAST of MELO3C031025 vs. NCBI nr
Match: XP_011651927.1 (ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [Cucumis sativus])

HSP 1 Score: 318.2 bits (814), Expect = 6.0e-83
Identity = 154/237 (64.98%), Postives = 182/237 (76.79%), Query Frame = 0


Query: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCND-GTQCHAQPQPEFTIHGLWPSN 60
           MAQH FILCLLS LLFVNG PFDFFQLV+QWGPN C+  G++C+AQPQP FTIHGLWPSN
Sbjct: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSN 60

Query: 61  FSNARLFC-ATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNI 120
           FS   + C  T+KLF  G+++ LE  L++YWPDVI+G N  FW +EW+KH  CIDPPF I
Sbjct: 61  FSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPPFTI 120

Query: 121 TQYFQLSLDH--HRTYDLLKILN--GVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRC 180
             YF +SL H  ++TYDLL ILN  G+ H+     +  L  +++L+PI NAT    GIRC
Sbjct: 121 FWYFDISLRHRMNKTYDLLTILNDAGLSHQT----HKDLISQNVLDPIKNATGMEPGIRC 180

Query: 181 NKNGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSC-NQPKFVWLLPQQSSDGVPDY 231
           N NGITKKLQL EIVLCF N+GTTLIDCP FVSN+C +QPKFVWLLPQQSS GVPDY
Sbjct: 181 NINGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDY 233

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BLAST of MELO3C031025 vs. NCBI nr
Match: KAE8651058.1 (hypothetical protein Csa_002465 [Cucumis sativus])

HSP 1 Score: 233.0 bits (593), Expect = 2.5e-57
Identity = 118/221 (53.39%), Postives = 146/221 (66.06%), Query Frame = 0


Query: 1   MAQHTFILCLLSLLLFVN-GYPFDFFQLVEQWGPNTCNDGTQ---CHAQPQPEFTIHGLW 60
           M Q   I+  LSL++FVN G PF+FFQ VEQWGPN CN G +   CH  PQ +FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPF 120
           PSN SNA L C ++ ++   ++  LEG L+  WPD+  GNN  FW +EW  HG C DPPF
Sbjct: 61  PSNHSNAHLLCQSKTIY--SKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPF 120

Query: 121 NITQYFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNK 180
           ++ QYFQ+SL+  R +DLL IL      N Q   N LA ++I+ PI   T K  GIRCNK
Sbjct: 121 SLFQYFQISLNLLRKFDLLTILKAA-GLNPQTSQN-LAIQNIMAPIQRTTKKYPGIRCNK 180

Query: 181 NGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVW 218
           N  T K QL+EIVLC + +G TLIDCP FVSN+C +  FVW
Sbjct: 181 NVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAK-SFVW 216

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BLAST of MELO3C031025 vs. NCBI nr
Match: XP_031738690.1 (ribonuclease MC-like [Cucumis sativus])

HSP 1 Score: 233.0 bits (593), Expect = 2.5e-57
Identity = 118/221 (53.39%), Postives = 146/221 (66.06%), Query Frame = 0


Query: 1   MAQHTFILCLLSLLLFVN-GYPFDFFQLVEQWGPNTCNDGTQ---CHAQPQPEFTIHGLW 60
           M Q   I+  LSL++FVN G PF+FFQ VEQWGPN CN G +   CH  PQ +FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPF 120
           PSN SNA L C ++ ++   ++  LEG L+  WPD+  GNN  FW +EW  HG C DPPF
Sbjct: 61  PSNHSNAHLLCQSKTIY--SKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPF 120

Query: 121 NITQYFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNK 180
           ++ QYFQ+SL+  R +DLL IL      N Q   N LA ++I+ PI   T K  GIRCNK
Sbjct: 121 SLFQYFQISLNLLRKFDLLTILKAA-GLNPQTSQN-LAIQNIMAPIQRTTKKYPGIRCNK 180

Query: 181 NGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVW 218
           N  T K QL+EIVLC + +G TLIDCP FVSN+C +  FVW
Sbjct: 181 NVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAK-SFVW 216

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BLAST of MELO3C031025 vs. NCBI nr
Match: XP_031738547.1 (ribonuclease MC-like [Cucumis sativus] >XP_031739193.1 ribonuclease MC-like [Cucumis sativus] >KAE8651059.1 hypothetical protein Csa_001394 [Cucumis sativus] >KAE8651065.1 hypothetical protein Csa_001488 [Cucumis sativus])

HSP 1 Score: 216.9 bits (551), Expect = 1.9e-52
Identity = 109/221 (49.32%), Postives = 141/221 (63.80%), Query Frame = 0


Query: 1   MAQHTFILCLLSLLLFVN-GYPFDFFQLVEQWGPNTCNDGTQ---CHAQPQPEFTIHGLW 60
           M Q   I+  LSL++FVN G PF+FFQ VEQWGPN CN G +   CH  PQ +FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPF 120
           PSN SNA L C  + +    ++ LLE  L+  WPD+  G N  FW++EW  HG C DPPF
Sbjct: 61  PSNHSNAHLLC--QSITKYTKVLLLERQLNISWPDLKGGMNNEFWTHEWNFHGKCSDPPF 120

Query: 121 NITQYFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNK 180
           ++ QYFQ+SL+  + +D+L IL       +   N G+  ++I+ PI   T K  GIRC K
Sbjct: 121 SLFQYFQMSLNLLQKFDVLAILKAAGLNPQTSQNIGI--QNIMTPIQRTTKKYPGIRCYK 180

Query: 181 NGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVW 218
           N  T K QL+EIVLC + +G TLIDCP FVS++C +   VW
Sbjct: 181 NVKTGKSQLNEIVLCLEKDGATLIDCPTFVSSTCAK-SIVW 216

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BLAST of MELO3C031025 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 6.1e-30
Identity = 66/186 (35.48%), Postives = 92/186 (49.46%), Query Frame = 0


Query: 22  FDFFQLVEQWGPNTCN--DGTQCHAQPQPEFTIHGLWPSNFSNARLFCATRKLFDNGQIS 81
           FD F  V+QW P  C+      C       FTIHGLWP     +   C     FD  +IS
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 82  LLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNITQYFQLSLDHHRTYDLLKILN 141
            L+  L+  WP+V+  NN+ FWS+EW KHG C +  FN   YF+L++D    YD++    
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDII---- 120

Query: 142 GVWHRNRQMPNNGLAPKDILNPIFNAT-NKTAGIRCNKNGITKKLQLHEIVLCFDNNGTT 201
           G    +   PN     +  +     A   K  G+RC  +  TK   L ++V CF  +G+T
Sbjct: 121 GALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGST 180

Query: 202 LIDCPK 205
           LIDC +
Sbjct: 181 LIDCTR 182

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BLAST of MELO3C031025 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 1.0e-24
Identity = 66/197 (33.50%), Postives = 97/197 (49.24%), Query Frame = 0


Query: 22  FDFFQLVEQWGPNTCNDGTQC----HAQPQPEFTIHGLWPSNFSNA-RLFCATRKLFDNG 81
           FDFF  V+QW  + C+    C      +P  +F IHGLWP+N        C     +D  
Sbjct: 29  FDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWPNNNDGTYPSNCDPNSPYDQS 88

Query: 82  QISLLEGDLDRYWPDVI--NGNNRGFWSNEWKKHGACIDPPF-NITQYFQLSLDHHRTYD 141
           QIS L   + + WP +   +G+   FWS+EW+KHG C +    N   YF+ +LD     D
Sbjct: 89  QISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAESVLTNQHAYFKKALDLKNQID 148

Query: 142 LLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNKNGITKKLQLHEIVLCFD 201
           LL IL G    +           +I N I +A   T  I+CN +  +   QL+++ +C D
Sbjct: 149 LLSILQGA---DIHPDGESYDLVNIRNAIKSAIGYTPWIQCNVDQ-SGNSQLYQVYICVD 208

Query: 202 NNGTTLIDCPKFVSNSC 211
            +G++LI+CP F    C
Sbjct: 209 GSGSSLIECPIFPGGKC 221

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BLAST of MELO3C031025 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.9e-24
Identity = 67/219 (30.59%), Postives = 108/219 (49.32%), Query Frame = 0


Query: 8   LCLLSLLLFV--------NGYPFDFFQLVEQWGPNTCNDGTQC----HAQPQPEFTIHGL 67
           LCL+SLL+ +        +   FDFF  V+QW  + C+   +C      +P  +F IHGL
Sbjct: 8   LCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGL 67

Query: 68  WPSNFSNARL--FCATRKLFDNGQISLLEGDLDRYWPDVI--NGNNRGFWSNEWKKHGAC 127
           WP N+ +      C   K FD+  IS L   + + WP +   +G+   FW +EW+KHG C
Sbjct: 68  WP-NYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTC 127

Query: 128 IDPPFNITQYFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAG 187
            +   +  +YFQ +L+  +  +LL  L               + + I + I  +   T  
Sbjct: 128 SESVIDQHEYFQTALNLKQKTNLLGALT---KAGINPDGKSYSLESIRDSIKESIGFTPW 187

Query: 188 IRCNKNGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSC 211
           + CN++G +   QL+++ LC D +G+ LI+CP F    C
Sbjct: 188 VECNRDG-SGNSQLYQVYLCVDRSGSGLIECPVFPHGKC 221

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BLAST of MELO3C031025 vs. ExPASy Swiss-Prot
Match: Q38717 (Ribonuclease S-4 OS=Antirrhinum hispanicum OX=49039 GN=S4 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 9.5e-23
Identity = 61/189 (32.28%), Postives = 95/189 (50.26%), Query Frame = 0


Query: 23  DFFQLVEQWGPNTC-NDGTQCHAQPQP-EFTIHGLWPSNFSNARLFCATRKLFDNGQISL 82
           D+ +LV QW  + C  +  +C   P P  FTIHGLWP N++       T   F     + 
Sbjct: 34  DYLKLVLQWPKSFCLINSRKCQRNPLPSNFTIHGLWPDNYTRQAPQSCTTNNFQRFTDTD 93

Query: 83  LEGDLDRYWPDV----INGNNRGFWSNEWKKHGACIDPPFNITQYFQLSLDHHRTYDLLK 142
           +E  ++  WPD+    I G +  FW ++W+KHG+C  PP     YF  +L+     D+L 
Sbjct: 94  IEQRMEESWPDLKQQSIAGLSYNFWQDQWRKHGSCCFPPHESEIYFLKALELKDRLDVLT 153

Query: 143 IL-NGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNKNGITKKLQLHEIVLCFDNN 202
           IL N  ++     P + L    + N I  A  KT  ++C ++       L E+V+C DNN
Sbjct: 154 ILENNNFNPGTPQPFSVLR---VFNTISRAIGKTPILKCAQS------YLKEVVICVDNN 213

Query: 203 GTTLIDCPK 205
           G +++ CP+
Sbjct: 214 GASVVHCPR 213

[Close]

BLAST of MELO3C031025 vs. ExPASy Swiss-Prot
Match: O80325 (Ribonuclease S-7 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 4.0e-21
Identity = 67/205 (32.68%), Postives = 103/205 (50.24%), Query Frame = 0


Query: 5   TFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCND-GTQCHAQPQPEFTIHGLWPSNFSNA 64
           T +  L+ L+L  +   +D+FQ  +Q+ P  CN   T C   P   FT+HGLWPSN +  
Sbjct: 11  TMVFSLIVLILSSSTVGYDYFQFTQQYQPAVCNSKPTPCKDPPDKLFTVHGLWPSNLNGP 70

Query: 65  RLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNR-GFWSNEWKKHGACIDPP-FNITQY 124
                T    +  +I  ++  L   WP+V++  N  GFW+ +W KHG+C  P   N T Y
Sbjct: 71  HPENCTNATVNPHRIKNIQAQLKIIWPNVLDRTNHVGFWNKQWIKHGSCGYPAIMNDTHY 130

Query: 125 FQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAP-KDILNPIFNAT-NKTAGIRCNKN-G 184
           FQ  ++ + T    + ++ +  + +  P     P   I N I N+T NK    +C KN G
Sbjct: 131 FQTVINMYITQK--QNVSEILSKAKIEPLGIQRPLVHIENAIRNSTNNKKPKFKCQKNSG 190

Query: 185 ITKKLQLHEIVLCFDNNGTTLIDCP 204
           +T   +L E+ LC D + T   +CP
Sbjct: 191 VT---ELVEVGLCSDGSLTQFRNCP 210

[Close]

BLAST of MELO3C031025 vs. ExPASy TrEMBL
Match: A0A5D3DP91 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002140 PE=3 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.4e-141
Identity = 231/231 (100.00%), Postives = 231/231 (100.00%), Query Frame = 0


Query: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCNDGTQCHAQPQPEFTIHGLWPSNF 60
           MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCNDGTQCHAQPQPEFTIHGLWPSNF
Sbjct: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCNDGTQCHAQPQPEFTIHGLWPSNF 60

Query: 61  SNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNITQ 120
           SNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNITQ
Sbjct: 61  SNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNITQ 120

Query: 121 YFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNKNGIT 180
           YFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNKNGIT
Sbjct: 121 YFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNKNGIT 180

Query: 181 KKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDYA 232
           KKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDYA
Sbjct: 181 KKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDYA 231

[Close]

BLAST of MELO3C031025 vs. ExPASy TrEMBL
Match: A0A0A0LAB0 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.9e-83
Identity = 154/237 (64.98%), Postives = 182/237 (76.79%), Query Frame = 0


Query: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCND-GTQCHAQPQPEFTIHGLWPSN 60
           MAQH FILCLLS LLFVNG PFDFFQLV+QWGPN C+  G++C+AQPQP FTIHGLWPSN
Sbjct: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAGSKCYAQPQPMFTIHGLWPSN 60

Query: 61  FSNARLFC-ATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNI 120
           FS   + C  T+KLF  G+++ LE  L++YWPDVI+G N  FW +EW+KH  CIDPPF I
Sbjct: 61  FSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKNSNFWEHEWRKHADCIDPPFTI 120

Query: 121 TQYFQLSLDH--HRTYDLLKILN--GVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRC 180
             YF +SL H  ++TYDLL ILN  G+ H+     +  L  +++L+PI NAT    GIRC
Sbjct: 121 FWYFDISLRHRMNKTYDLLTILNDAGLSHQT----HKDLISQNVLDPIKNATGMEPGIRC 180

Query: 181 NKNGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSC-NQPKFVWLLPQQSSDGVPDY 231
           N NGITKKLQL EIVLCF N+GTTLIDCP FVSN+C +QPKFVWLLPQQSS GVPDY
Sbjct: 181 NINGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDY 233

[Close]

BLAST of MELO3C031025 vs. ExPASy TrEMBL
Match: A0A0A0LAN6 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 1.2e-57
Identity = 118/221 (53.39%), Postives = 146/221 (66.06%), Query Frame = 0


Query: 1   MAQHTFILCLLSLLLFVN-GYPFDFFQLVEQWGPNTCNDGTQ---CHAQPQPEFTIHGLW 60
           M Q   I+  LSL++FVN G PF+FFQ VEQWGPN CN G +   CH  PQ +FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPF 120
           PSN SNA L C ++ ++   ++  LEG L+  WPD+  GNN  FW +EW  HG C DPPF
Sbjct: 61  PSNHSNAHLLCQSKTIY--SKVISLEGQLNISWPDLKYGNNDKFWGHEWDTHGKCSDPPF 120

Query: 121 NITQYFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAGIRCNK 180
           ++ QYFQ+SL+  R +DLL IL      N Q   N LA ++I+ PI   T K  GIRCNK
Sbjct: 121 SLFQYFQISLNLLRKFDLLTILKAA-GLNPQTSQN-LAIQNIMAPIQRTTKKYPGIRCNK 180

Query: 181 NGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVW 218
           N  T K QL+EIVLC + +G TLIDCP FVSN+C +  FVW
Sbjct: 181 NVKTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAK-SFVW 216

[Close]

BLAST of MELO3C031025 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 1.6e-49
Identity = 110/230 (47.83%), Postives = 137/230 (59.57%), Query Frame = 0


Query: 1   MAQHTFILCLLSLLLFVN--GYPFDFFQLVEQWGPNTCNDGTQCHAQPQPEFTIHGLWPS 60
           MAQ  FI+ +   +L  +     FD+FQ+V+QW P TC+ G +C+A P   FTIHGLWPS
Sbjct: 1   MAQAPFIVSIFKFVLLTSCVKAQFDYFQMVQQWPPATCS-GVRCYANPPAMFTIHGLWPS 60

Query: 61  NFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNI 120
           N+SN  L CA +  F+  QI+ L   L+ YWPDVI+GNN+ FW +EW  HG C DPPFN 
Sbjct: 61  NYSNVPLVCAGQP-FNPAQIATLVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQ 120

Query: 121 TQYFQ--LSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPK--------DILNPIFNATNK 180
            QYFQ  L++  +  YDLL ILN            GL P          I   I  AT K
Sbjct: 121 LQYFQTTLNIRTNHNYDLLAILN----------TAGLGPTATNTRQYGAIEGAIQAATGK 180

Query: 181 TAGIRCNKNGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWL 219
             G+RCNKN  +KK QL EI+LCFD NG TLIDC +F   +C   +FVWL
Sbjct: 181 KPGLRCNKNAQSKKKQLFEIILCFDKNGVTLIDCTQFAGITC-PSQFVWL 217

[Close]

BLAST of MELO3C031025 vs. ExPASy TrEMBL
Match: A0A6J1IAR7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 3.6e-49
Identity = 108/225 (48.00%), Postives = 136/225 (60.44%), Query Frame = 0


Query: 6   FILCLLSLLLFVNGY--PFDFFQLVEQWGPNTCNDGTQCHAQPQPEFTIHGLWPSNFSNA 65
           FI+ + +L+L   G    FD+FQ+V+QW P TC+ G +C+A P   FTIHGLWPS +SN 
Sbjct: 7   FIVSIFNLVLLTIGVKAQFDYFQMVQQWPPATCS-GVRCYANPPAMFTIHGLWPSTYSNV 66

Query: 66  RLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRGFWSNEWKKHGACIDPPFNITQYFQ 125
              CA +  FD  QI+ LE  L+ YWPDVINGNN+ FW +EW  HG C DPPFN  QYFQ
Sbjct: 67  PPVCAGQP-FDRAQIATLEPQLNTYWPDVINGNNQRFWKHEWDNHGTCSDPPFNQLQYFQ 126

Query: 126 --LSLDHHRTYDLLKILNGVWHRNRQMPNNGLAP--------KDILNPIFNATNKTAGIR 185
             L++  +  YDLL ILN            GL P        + I   I  AT K  G+R
Sbjct: 127 TTLNIRTNHNYDLLAILN----------TAGLGPTATTTRQYQAIEGAIQAATGKKPGLR 186

Query: 186 CNKNGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWL 219
           CNKN  +K+ QL EI+LCFD NG TLI+C +F   +C   +FVWL
Sbjct: 187 CNKNAQSKRKQLFEIILCFDKNGVTLINCTQFAGITC-PSQFVWL 218

[Close]

BLAST of MELO3C031025 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 113.2 bits (282), Expect = 2.7e-25
Identity = 67/219 (30.59%), Postives = 108/219 (49.32%), Query Frame = 0


Query: 8   LCLLSLLLFV--------NGYPFDFFQLVEQWGPNTCNDGTQC----HAQPQPEFTIHGL 67
           LCL+SLL+ +        +   FDFF  V+QW  + C+   +C      +P  +F IHGL
Sbjct: 8   LCLISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGL 67

Query: 68  WPSNFSNARL--FCATRKLFDNGQISLLEGDLDRYWPDVI--NGNNRGFWSNEWKKHGAC 127
           WP N+ +      C   K FD+  IS L   + + WP +   +G+   FW +EW+KHG C
Sbjct: 68  WP-NYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTC 127

Query: 128 IDPPFNITQYFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAPKDILNPIFNATNKTAG 187
            +   +  +YFQ +L+  +  +LL  L               + + I + I  +   T  
Sbjct: 128 SESVIDQHEYFQTALNLKQKTNLLGALT---KAGINPDGKSYSLESIRDSIKESIGFTPW 187

Query: 188 IRCNKNGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSC 211
           + CN++G +   QL+++ LC D +G+ LI+CP F    C
Sbjct: 188 VECNRDG-SGNSQLYQVYLCVDRSGSGLIECPVFPHGKC 221

[Close]

BLAST of MELO3C031025 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 110.2 bits (274), Expect = 2.3e-24
Identity = 74/228 (32.46%), Postives = 102/228 (44.74%), Query Frame = 0


Query: 6   FILCLLSLLLFVNGYPFDFFQLVEQWGPNTCNDGTQC----HAQPQPEFTIHGLWPSNFS 65
           FIL L  L +      FDFF  V QW    C+    C      +P  +F IHGLWP+  +
Sbjct: 6   FILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKT 65

Query: 66  NA-RLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRG--FWSNEWKKHGACIDPPFNI 125
                 C     FD+ ++S L  DL R WP +   +N G  FW++EW+KHG C +   + 
Sbjct: 66  GGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQ 125

Query: 126 TQYFQLSLDHHRTYDLLKILNGVWHRNRQMPNNGLAP-------KDILNPIFNATNKTAG 185
             YF+  L   +  +LL  L           N G+ P       KDI N I        G
Sbjct: 126 HDYFEAGLKLKQKANLLHALT----------NAGIKPDDKFYEMKDIENTIKQVVGFAPG 185

Query: 186 IRCNKNGITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQ----PKF 216
           I CNK+  +   QL++I LC D + +  I+CP      C+     PKF
Sbjct: 186 IECNKDS-SHNSQLYQIYLCVDTSASKFINCPVMPHGRCDSRVQFPKF 222

[Close]

BLAST of MELO3C031025 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 102.1 bits (253), Expect = 6.3e-22
Identity = 67/216 (31.02%), Postives = 92/216 (42.59%), Query Frame = 0


Query: 22  FDFFQLVEQWGPNTCNDGTQC----HAQPQPEFTIHGLWPSNFSNAR--LFCATRKLFDN 81
           F+FF  V  W    C+    C          +F IHGLWP  F+N     FC    LFD 
Sbjct: 26  FNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGLWP-QFNNGTWPAFCDQTNLFDI 85

Query: 82  GQISLLEGDLDRYWPD----VINGNNRGFWSNEWKKHGACIDPPFNITQYFQLSLDHHRT 141
            +IS L   +++ W +        N    W +EW KHG C+   F+   YF+ +L     
Sbjct: 86  SKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGTCVQSIFDQHSYFRTNLKFKHK 145

Query: 142 YDLLKIL--NGVWHRNRQMPNNGLAPKD-ILNPIFNATNKTAGIRCNKNGITKKLQLHEI 201
             LL IL   G+       PN+G    D I N I  A     GI CN++ +    QL +I
Sbjct: 146 VHLLNILIQKGI------KPNDGFYSLDEIKNAIKCAIGFAPGIECNED-VKGNKQLFQI 205

Query: 202 VLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSS 225
            +C DN     ++CP     SC        LP++ S
Sbjct: 206 YICLDNYAKEFVECPYVPDKSCASKIKFPSLPERDS 233

The following BLAST results are available for this feature:

BLAST of MELO3C031025 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK25090.1	2.8e-141	100.00	S-RNase [Cucumis melo var. makuwa]	[more]
XP_011651927.1	6.0e-83	64.98	ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [C...	[more]
KAE8651058.1	2.5e-57	53.39	hypothetical protein Csa_002465 [Cucumis sativus]	[more]
XP_031738690.1	2.5e-57	53.39	ribonuclease MC-like [Cucumis sativus]	[more]
XP_031738547.1	1.9e-52	49.32	ribonuclease MC-like [Cucumis sativus] >XP_031739193.1 ribonuclease MC-like [Cuc...	[more]

BLAST of MELO3C031025 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	6.1e-30	35.48	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P80022	1.0e-24	33.50	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P42813	3.9e-24	30.59	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
Q38717	9.5e-23	32.28	Ribonuclease S-4 OS=Antirrhinum hispanicum OX=49039 GN=S4 PE=2 SV=1	[more]
O80325	4.0e-21	32.68	Ribonuclease S-7 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]

BLAST of MELO3C031025 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DP91	1.4e-141	100.00	Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00...	[more]
A0A0A0LAB0	2.9e-83	64.98	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1	[more]
A0A0A0LAN6	1.2e-57	53.39	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1	[more]
A0A6J1EXD5	1.6e-49	47.83	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1	[more]
A0A6J1IAR7	3.6e-49	48.00	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1	[more]

BLAST of MELO3C031025 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	2.7e-25	30.59	ribonuclease 1	[more]
AT1G26820.1	2.3e-24	32.46	ribonuclease 3	[more]
AT1G14210.1	6.3e-22	31.02	Ribonuclease T2 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 22..202 e-value: 1.7E-34 score: 119.6
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 10..213
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 18..216 e-value: 3.1E-46 score: 160.0
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 19..215
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 10..213
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 50..57
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 22..217 e-value: 1.50549E-52 score: 166.354

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C031025.1	MELO3C031025.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding