MELO3C030349 (gene) Melon (DHL92) v4

BLAST of MELO3C030349 vs. NCBI nr
Match: KAE8651450.1 (hypothetical protein Csa_001467 [Cucumis sativus])

HSP 1 Score: 384.4 bits (986), Expect = 6.5e-103
Identity = 184/215 (85.58%), Postives = 195/215 (90.70%), Query Frame = 0

Query: 1    MVATNENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESI 60
            +VATNENG+K YNN+TIIDL+L +FFAGHNTPAIAA+WALLHISQNP IFQMAKEEQESI
Sbjct: 840  IVATNENGSKPYNNSTIIDLILGIFFAGHNTPAIAAMWALLHISQNPHIFQMAKEEQESI 899

Query: 61   IRQRPSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTV 120
            IRQRPSTQKGLTFQEIKQMKYL KFINEVLRRNTVAPTNF           YT+PKGWTV
Sbjct: 900  IRQRPSTQKGLTFQEIKQMKYLTKFINEVLRRNTVAPTNFRKARTYVNINGYTIPKGWTV 959

Query: 121  QIWGVAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLH 180
            QIW VA+HMDS+IYSN QEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSEL KLEITILLH
Sbjct: 960  QIWSVAIHMDSQIYSNSQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLH 1019

Query: 181  HFILNYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
            HFILNYKMELV+QNC VTHLPSPKP DNCLC+IIR
Sbjct: 1020 HFILNYKMELVDQNCKVTHLPSPKPRDNCLCKIIR 1054

BLAST of MELO3C030349 vs. NCBI nr
Match: XP_011652660.1 (beta-amyrin 11-oxidase [Cucumis sativus])

HSP 1 Score: 384.4 bits (986), Expect = 6.5e-103
Identity = 184/215 (85.58%), Postives = 195/215 (90.70%), Query Frame = 0

Query: 1   MVATNENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESI 60
           +VATNENG+K YNN+TIIDL+L +FFAGHNTPAIAA+WALLHISQNP IFQMAKEEQESI
Sbjct: 274 IVATNENGSKPYNNSTIIDLILGIFFAGHNTPAIAAMWALLHISQNPHIFQMAKEEQESI 333

Query: 61  IRQRPSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTV 120
           IRQRPSTQKGLTFQEIKQMKYL KFINEVLRRNTVAPTNF           YT+PKGWTV
Sbjct: 334 IRQRPSTQKGLTFQEIKQMKYLTKFINEVLRRNTVAPTNFRKARTYVNINGYTIPKGWTV 393

Query: 121 QIWGVAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLH 180
           QIW VA+HMDS+IYSN QEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSEL KLEITILLH
Sbjct: 394 QIWSVAIHMDSQIYSNSQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLH 453

Query: 181 HFILNYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
           HFILNYKMELV+QNC VTHLPSPKP DNCLC+IIR
Sbjct: 454 HFILNYKMELVDQNCKVTHLPSPKPRDNCLCKIIR 488

BLAST of MELO3C030349 vs. NCBI nr
Match: XP_004148514.3 (beta-amyrin 11-oxidase-like isoform X1 [Cucumis sativus])

HSP 1 Score: 354.0 bits (907), Expect = 9.4e-94
Identity = 172/215 (80.00%), Postives = 189/215 (87.91%), Query Frame = 0

Query: 1   MVATNENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESI 60
           +VA NENG+KSY+++TIIDLLL  FFAGHNTPA AA+WALLHISQNP IFQMAKEEQESI
Sbjct: 275 IVAKNENGSKSYSDDTIIDLLLGTFFAGHNTPATAAMWALLHISQNPHIFQMAKEEQESI 334

Query: 61  IRQRPSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTV 120
           IRQRPSTQKGLTFQEIKQMK+L KFINEVLRRNTVAPTNF           YT+PKGWTV
Sbjct: 335 IRQRPSTQKGLTFQEIKQMKFLTKFINEVLRRNTVAPTNFRKAKTNVNINGYTIPKGWTV 394

Query: 121 QIWGVAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLH 180
           QIW VAVHMDS+IYSNPQ+FDPSRWDNYTPKPGEFIPFGLGS+FCPGSEL KLE+TILLH
Sbjct: 395 QIWSVAVHMDSQIYSNPQDFDPSRWDNYTPKPGEFIPFGLGSKFCPGSELAKLEMTILLH 454

Query: 181 HFILNYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
           HF+LNYK+E V+QNCN T LPS KP+   LCRI+R
Sbjct: 455 HFVLNYKVEPVDQNCNDTRLPSAKPMG--LCRIVR 487

BLAST of MELO3C030349 vs. NCBI nr
Match: XP_004148515.2 (beta-amyrin 11-oxidase [Cucumis sativus] >AIT72036.1 cytochrome P450 [Cucumis sativus] >KGN60415.1 hypothetical protein Csa_001840 [Cucumis sativus])

HSP 1 Score: 330.5 bits (846), Expect = 1.1e-86
Identity = 161/215 (74.88%), Postives = 176/215 (81.86%), Query Frame = 0

Query: 1   MVATNENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESI 60
           +VA NENG K Y+NN I DL+L +FFAGH+TPAIAA WALLHISQNP +FQ AKEEQESI
Sbjct: 274 VVAINENGAKLYSNNFIRDLILGIFFAGHSTPAIAACWALLHISQNPHVFQKAKEEQESI 333

Query: 61  IRQRPSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFHYT----------VPKGWTV 120
           IRQRPS QKGLT  EIKQMKYL K INEVLRRNT+  TNF  T          +PKGWTV
Sbjct: 334 IRQRPSVQKGLTLNEIKQMKYLTKIINEVLRRNTITATNFRETKTDVNINGHFIPKGWTV 393

Query: 121 QIWGVAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLH 180
           QIW +AVHMD +IYSNPQEF+PSRWDNYTPKPGEFIPFGLGSRFCPGSEL KLEITILLH
Sbjct: 394 QIWNIAVHMDPQIYSNPQEFNPSRWDNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLH 453

Query: 181 HFILNYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
           HFILNYKME VN   N   +PSP+P +NCLC+IIR
Sbjct: 454 HFILNYKMEGVNLESNNNQMPSPRP-ENCLCKIIR 487

BLAST of MELO3C030349 vs. NCBI nr
Match: XP_022952843.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 322.4 bits (825), Expect = 3.0e-84
Identity = 151/215 (70.23%), Postives = 176/215 (81.86%), Query Frame = 0

Query: 1   MVATNENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESI 60
           ++A +ENG K Y+NN IIDLLL L  AGH+TPA AA+WAL+HIS+ P +F  AKEEQESI
Sbjct: 270 IMARDENGMKLYDNNAIIDLLLGLLHAGHHTPAYAAMWALVHISEKPHVFHKAKEEQESI 329

Query: 61  IRQRPSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTV 120
           I+QRPSTQKGLTFQEIKQMKYLMKFINE+LRRNT+A  +F           Y +PKGW V
Sbjct: 330 IKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNTIAVASFRKAKKDVNINDYIIPKGWNV 389

Query: 121 QIWGVAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLH 180
            IW  A+HMD ++YSNPQEFDPSRW+NY+PKPG F+PFG+G+RFCPGSELTKLEITILLH
Sbjct: 390 LIWTRAIHMDPQLYSNPQEFDPSRWENYSPKPGTFLPFGIGTRFCPGSELTKLEITILLH 449

Query: 181 HFILNYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
           HFILNYKME VN  C +THLP+PK VDNCLC I +
Sbjct: 450 HFILNYKMERVNPKCCITHLPAPKLVDNCLCTITK 484

BLAST of MELO3C030349 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 2.8e-56
Identity = 106/211 (50.24%), Postives = 143/211 (67.77%), Query Frame = 0

Query: 5   NENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESIIRQR 64
           +ENG K   +  I DLL+ L FAGH + A + +W++ +++Q+P I + AKEEQE I R R
Sbjct: 280 DENGRK-LEDEDISDLLIGLLFAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTR 339

Query: 65  PSTQKGLTFQEIKQMKYLMKFINEVLR---------RNTVAPTNFH-YTVPKGWTVQIWG 124
            S+QK L+ +EIKQM YL + I+E LR         R   A  N + Y +PKGW V IW 
Sbjct: 340 FSSQKQLSLKEIKQMVYLSQVIDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWA 399

Query: 125 VAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLHHFIL 184
            A+HMDS  Y NP+EF+PSRWD+Y  K G F+PFG GSR CPG++L KLEI+I LH+F+ 
Sbjct: 400 RAIHMDSEYYPNPEEFNPSRWDDYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLR 459

Query: 185 NYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
           NY++E +N  C+VT LP  KP DNCL ++I+
Sbjct: 460 NYRLERINPECHVTSLPVSKPTDNCLAKVIK 489

BLAST of MELO3C030349 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 4.9e-53
Identity = 96/221 (43.44%), Postives = 143/221 (64.71%), Query Frame = 0

Query: 1   MVATNENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESI 60
           ++A  + G +   ++ IID+L+    AGH +     +WA + + +NP I   AK EQE+I
Sbjct: 279 LIAVEDEGGRRLQDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPEILAKAKAEQEAI 338

Query: 61  IRQRPSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTV 120
           +R  P  QKGLT ++ ++M YL + ++E LR   ++  +F           Y +PKGW V
Sbjct: 339 MRSIPPGQKGLTLRDFRKMAYLSQVVDETLRFVNISFVSFRQATRDVFVNGYLIPKGWKV 398

Query: 121 QIWGVAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLH 180
           Q+W  +VHMD ++Y +P++FDPSRW+   P+ G F+PFGLG+R CPG++L KLEI++ LH
Sbjct: 399 QLWYRSVHMDPQVYPDPKKFDPSRWEGPPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLH 458

Query: 181 HFILNYKMELVNQNCNVTHLPSPKPVDNCLCRIIRGLSKSY 212
           HF+L YK+   N NC V +LP P+PVDNCL +I R LS S+
Sbjct: 459 HFLLGYKLTRKNPNCRVRYLPHPRPVDNCLAKITR-LSSSH 498

BLAST of MELO3C030349 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 9.2e-52
Identity = 93/213 (43.66%), Postives = 142/213 (66.67%), Query Frame = 0

Query: 3   ATNENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESIIR 62
           A +E G +  +++ IID+L+    AGH +     +WA + + +NP +F  AK EQE+I+R
Sbjct: 305 AQDERG-RHLDDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPDMFARAKAEQEAIMR 364

Query: 63  QRPSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTVQI 122
             PS+Q+GLT ++ ++M+YL + I+E LR   ++  +F           Y +PKGW VQ+
Sbjct: 365 SIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVSFRQATRDVFVNGYLIPKGWKVQL 424

Query: 123 WGVAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLHHF 182
           W  +VHMD ++Y +P +FDPSRW+ ++P+ G F+ FGLG+R CPG++L KLEI++ LHHF
Sbjct: 425 WYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTFLAFGLGARLCPGNDLAKLEISVFLHHF 484

Query: 183 ILNYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
           +L YK+   N  C V +LP P+PVDNCL +I R
Sbjct: 485 LLGYKLARTNPRCRVRYLPHPRPVDNCLAKITR 516

BLAST of MELO3C030349 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 2.3e-50
Identity = 92/213 (43.19%), Postives = 137/213 (64.32%), Query Frame = 0

Query: 3   ATNENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESIIR 62
           A +E G +   ++ I+D+L+    AGH +     +WA + + +NP IF  AK EQE I+R
Sbjct: 290 AEDERG-RRLADDEIVDVLIMYLNAGHESSGHITMWATVFLQENPDIFARAKAEQEEIMR 349

Query: 63  QRPSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTVQI 122
             P+TQ GLT ++ K+M +L + ++E LR   ++  +F           Y +PKGW VQ+
Sbjct: 350 SIPATQNGLTLRDFKKMHFLSQVVDETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQL 409

Query: 123 WGVAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLHHF 182
           W  +VHMD ++Y +P+ F+PSRW+   PK G F+PFGLG+R CPG++L KLEI++ LHHF
Sbjct: 410 WYRSVHMDDQVYPDPKMFNPSRWEGPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHF 469

Query: 183 ILNYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
           +L YK++  N  C V +LP P+PVDNCL  I +
Sbjct: 470 LLGYKLKRANPKCRVRYLPHPRPVDNCLATITK 501

BLAST of MELO3C030349 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 195.3 bits (495), Expect = 7.3e-49
Identity = 100/211 (47.39%), Postives = 133/211 (63.03%), Query Frame = 0

Query: 5   NENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESIIRQR 64
           +ENG +  ++  IIDLLL    AGH +     +WA + + ++P I Q AKEEQE I+++R
Sbjct: 278 DENG-RVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKR 337

Query: 65  PSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTVQIWG 124
              QK LT +E ++M YL + I+E LR  T + T F           Y +PKGW V  W 
Sbjct: 338 APGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWF 397

Query: 125 VAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLHHFIL 184
             VH+D  IY +P++FDPSRW+ YTPK G F+PFGLGS  CPG++L KLEI+I LHHF+L
Sbjct: 398 RNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL 457

Query: 185 NYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
            Y++E  N  C V  LP  +P DNCL RI R
Sbjct: 458 KYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of MELO3C030349 vs. ExPASy TrEMBL
Match: A0A097IYP6 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G903550 PE=2 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 5.4e-87
Identity = 161/215 (74.88%), Postives = 176/215 (81.86%), Query Frame = 0

Query: 1   MVATNENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESI 60
           +VA NENG K Y+NN I DL+L +FFAGH+TPAIAA WALLHISQNP +FQ AKEEQESI
Sbjct: 274 VVAINENGAKLYSNNFIRDLILGIFFAGHSTPAIAACWALLHISQNPHVFQKAKEEQESI 333

Query: 61  IRQRPSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFHYT----------VPKGWTV 120
           IRQRPS QKGLT  EIKQMKYL K INEVLRRNT+  TNF  T          +PKGWTV
Sbjct: 334 IRQRPSVQKGLTLNEIKQMKYLTKIINEVLRRNTITATNFRETKTDVNINGHFIPKGWTV 393

Query: 121 QIWGVAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLH 180
           QIW +AVHMD +IYSNPQEF+PSRWDNYTPKPGEFIPFGLGSRFCPGSEL KLEITILLH
Sbjct: 394 QIWNIAVHMDPQIYSNPQEFNPSRWDNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLH 453

Query: 181 HFILNYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
           HFILNYKME VN   N   +PSP+P +NCLC+IIR
Sbjct: 454 HFILNYKMEGVNLESNNNQMPSPRP-ENCLCKIIR 487

BLAST of MELO3C030349 vs. ExPASy TrEMBL
Match: A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.5e-84
Identity = 151/215 (70.23%), Postives = 176/215 (81.86%), Query Frame = 0

Query: 1   MVATNENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESI 60
           ++A +ENG K Y+NN IIDLLL L  AGH+TPA AA+WAL+HIS+ P +F  AKEEQESI
Sbjct: 270 IMARDENGMKLYDNNAIIDLLLGLLHAGHHTPAYAAMWALVHISEKPHVFHKAKEEQESI 329

Query: 61  IRQRPSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTV 120
           I+QRPSTQKGLTFQEIKQMKYLMKFINE+LRRNT+A  +F           Y +PKGW V
Sbjct: 330 IKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNTIAVASFRKAKKDVNINDYIIPKGWNV 389

Query: 121 QIWGVAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLH 180
            IW  A+HMD ++YSNPQEFDPSRW+NY+PKPG F+PFG+G+RFCPGSELTKLEITILLH
Sbjct: 390 LIWTRAIHMDPQLYSNPQEFDPSRWENYSPKPGTFLPFGIGTRFCPGSELTKLEITILLH 449

Query: 181 HFILNYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
           HFILNYKME VN  C +THLP+PK VDNCLC I +
Sbjct: 450 HFILNYKMERVNPKCCITHLPAPKLVDNCLCTITK 484

BLAST of MELO3C030349 vs. ExPASy TrEMBL
Match: A0A6J1GJP8 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454912 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.8e-83
Identity = 148/215 (68.84%), Postives = 176/215 (81.86%), Query Frame = 0

Query: 1   MVATNENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESI 60
           ++A +ENG K Y+NN IIDLLL L   GH+TPA AA+WAL+HIS+ P +F  AKEEQESI
Sbjct: 270 IMARDENGMKLYDNNAIIDLLLGLLHGGHHTPAYAAMWALVHISETPHVFHKAKEEQESI 329

Query: 61  IRQRPSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTV 120
           ++QR STQKGLTFQEIKQMKYLMKFINE+LRRNT+A  +F           YT+PKGWTV
Sbjct: 330 LKQRSSTQKGLTFQEIKQMKYLMKFINELLRRNTIAVASFREAKTEININDYTIPKGWTV 389

Query: 121 QIWGVAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLH 180
            IW  A+HMD ++YSNP+EFDPSRW+N++PKPG FIPFG+G+RFCPGSELTKLE+TILLH
Sbjct: 390 LIWTRAIHMDPQLYSNPEEFDPSRWENHSPKPGTFIPFGIGTRFCPGSELTKLELTILLH 449

Query: 181 HFILNYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
           HFILNYKME VN  C +THLP+PK VDNCLC I +
Sbjct: 450 HFILNYKMERVNPKCCITHLPAPKLVDNCLCTITK 484

BLAST of MELO3C030349 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.6e-83
Identity = 149/215 (69.30%), Postives = 176/215 (81.86%), Query Frame = 0

Query: 1   MVATNENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESI 60
           ++A +ENG K Y+NNTIIDLLL L  AGH+TPA AA+WAL+HIS+ P IF  AKEEQESI
Sbjct: 271 IMARDENGMKLYDNNTIIDLLLGLLHAGHHTPAYAAMWALVHISEKPHIFHKAKEEQESI 330

Query: 61  IRQRPSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTV 120
           ++QRPSTQKGLTFQEIKQMKYLMKFINE+LRRNT+   +F           Y +PKGW+V
Sbjct: 331 LKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNTIVVASFRKAKTDININDYIIPKGWSV 390

Query: 121 QIWGVAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLH 180
            IW  AVHMD ++YSNP+EFDPSRW+N++PKPG FIPFG+G+RFCPGSELTKLEITILLH
Sbjct: 391 LIWTRAVHMDPQLYSNPEEFDPSRWENHSPKPGTFIPFGIGTRFCPGSELTKLEITILLH 450

Query: 181 HFILNYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
           HFILNYKME  N  C ++HLP+PK VDNCLC I +
Sbjct: 451 HFILNYKMERTNPKCCISHLPAPKLVDNCLCTITK 485

BLAST of MELO3C030349 vs. ExPASy TrEMBL
Match: A0A6J1I8I5 (beta-amyrin 11-oxidase-like OS=Cucurbita maxima OX=3661 GN=LOC111470631 PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 2.0e-81
Identity = 147/224 (65.62%), Postives = 175/224 (78.12%), Query Frame = 0

Query: 1   MVATNENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESI 60
           ++A +ENG K Y+NN IIDLLL L   GH+TPA AA+WAL+ I + P +F  AKEEQESI
Sbjct: 270 IMARDENGMKLYDNNAIIDLLLGLLHGGHHTPAYAAMWALVQIFETPHVFHKAKEEQESI 329

Query: 61  IRQRPSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH------------------- 120
           ++QRPSTQKGLTFQEIKQMKYLMKFINE+LRRNT+A  +F                    
Sbjct: 330 LKQRPSTQKGLTFQEIKQMKYLMKFINELLRRNTIAVASFREAKTDININGELFYYIVLD 389

Query: 121 YTVPKGWTVQIWGVAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELT 180
           YT+PKGWTV IW  A+HMD ++YSNP+EFDPSRW+N++PKPG FIPFG+G+RFCPGSELT
Sbjct: 390 YTIPKGWTVLIWTRAIHMDPQLYSNPKEFDPSRWENHSPKPGTFIPFGMGTRFCPGSELT 449

Query: 181 KLEITILLHHFILNYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
           KLE+TILLHHFILNYKME VN  C +THLP+PK VDNCLC I +
Sbjct: 450 KLELTILLHHFILNYKMERVNPKCCITHLPAPKLVDNCLCTITK 493

BLAST of MELO3C030349 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 195.3 bits (495), Expect = 5.2e-50
Identity = 100/211 (47.39%), Postives = 133/211 (63.03%), Query Frame = 0

Query: 5   NENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESIIRQR 64
           +ENG +  ++  IIDLLL    AGH +     +WA + + ++P I Q AKEEQE I+++R
Sbjct: 278 DENG-RVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKR 337

Query: 65  PSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTVQIWG 124
              QK LT +E ++M YL + I+E LR  T + T F           Y +PKGW V  W 
Sbjct: 338 APGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWF 397

Query: 125 VAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLHHFIL 184
             VH+D  IY +P++FDPSRW+ YTPK G F+PFGLGS  CPG++L KLEI+I LHHF+L
Sbjct: 398 RNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL 457

Query: 185 NYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
            Y++E  N  C V  LP  +P DNCL RI R
Sbjct: 458 KYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of MELO3C030349 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 195.3 bits (495), Expect = 5.2e-50
Identity = 100/211 (47.39%), Postives = 133/211 (63.03%), Query Frame = 0

Query: 5   NENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESIIRQR 64
           +ENG +  ++  IIDLLL    AGH +     +WA + + ++P I Q AKEEQE I+++R
Sbjct: 278 DENG-RVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPMILQKAKEEQERIVKKR 337

Query: 65  PSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTVQIWG 124
              QK LT +E ++M YL + I+E LR  T + T F           Y +PKGW V  W 
Sbjct: 338 APGQK-LTLKETREMVYLSQVIDETLRVITFSLTAFREAKSDVQMDGYIIPKGWKVLTWF 397

Query: 125 VAVHMDSRIYSNPQEFDPSRWDNYTPKPGEFIPFGLGSRFCPGSELTKLEITILLHHFIL 184
             VH+D  IY +P++FDPSRW+ YTPK G F+PFGLGS  CPG++L KLEI+I LHHF+L
Sbjct: 398 RNVHLDPEIYPDPKKFDPSRWEGYTPKAGTFLPFGLGSHLCPGNDLAKLEISIFLHHFLL 457

Query: 185 NYKMELVNQNCNVTHLPSPKPVDNCLCRIIR 206
            Y++E  N  C V  LP  +P DNCL RI R
Sbjct: 458 KYRVERSNPGCPVMFLPHNRPKDNCLARITR 486

BLAST of MELO3C030349 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 189.1 bits (479), Expect = 3.7e-48
Identity = 90/210 (42.86%), Postives = 134/210 (63.81%), Query Frame = 0

Query: 5   NENGTKSYNNNTIIDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESIIRQR 64
           +E+G K+ ++  IID+LL    AGH +     +WA + + ++P + Q AK EQE I++ R
Sbjct: 279 DEDG-KTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPEVLQRAKAEQEMILKSR 338

Query: 65  PSTQKGLTFQEIKQMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTVQIWG 124
           P  QKGL+ +E ++M++L + ++E LR  T + T F           Y +PKGW V  W 
Sbjct: 339 PEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVEMNGYLIPKGWKVLTWF 398

Query: 125 VAVHMDSRIYSNPQEFDPSRWDN-YTPKPGEFIPFGLGSRFCPGSELTKLEITILLHHFI 184
             VH+D  ++ +P++FDP+RWDN + PK G F+PFG GS  CPG++L KLEI+I LHHF+
Sbjct: 399 RDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLCPGNDLAKLEISIFLHHFL 458

Query: 185 LNYKMELVNQNCNVTHLPSPKPVDNCLCRI 204
           L Y+++  N  C V +LP  +P DNCL RI
Sbjct: 459 LKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

BLAST of MELO3C030349 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 126.7 bits (317), Expect = 2.3e-29
Identity = 65/186 (34.95%), Postives = 98/186 (52.69%), Query Frame = 0

Query: 18  IDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESIIRQRPSTQKGLTFQEIK 77
           +DL+  L FA   T ++A   A+  +S +P + +   EE E+I+R R     GLT++E K
Sbjct: 271 LDLMFVLLFASFETTSLALTLAIKFLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYK 330

Query: 78  QMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTVQIWGVAVHMDSRIYSNP 137
            M Y  +FINE  R   + P  F           YT+P GW V +   AVH++  +Y +P
Sbjct: 331 SMTYTFQFINETARLANIVPAIFRKALRDIKFKDYTIPAGWAVMVCPPAVHLNPEMYKDP 390

Query: 138 QEFDPSRWD--NYTPKPGEFIPFGLGSRFCPGSELTKLEITILLHHFILNYKMELVNQNC 192
             F+PSRW+    T     F+ FG G RFC G++ TKL++   LH  +  Y+ E + +  
Sbjct: 391 LVFNPSRWEGSKVTNASKHFMAFGGGMRFCVGTDFTKLQMAAFLHSLVTKYRWEEI-KGG 450

BLAST of MELO3C030349 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 126.7 bits (317), Expect = 2.3e-29
Identity = 65/186 (34.95%), Postives = 98/186 (52.69%), Query Frame = 0

Query: 18  IDLLLPLFFAGHNTPAIAAIWALLHISQNPRIFQMAKEEQESIIRQRPSTQKGLTFQEIK 77
           +DL+  L FA   T ++A   A+  +S +P + +   EE E+I+R R     GLT++E K
Sbjct: 277 LDLMFVLLFASFETTSLALTLAIKFLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYK 336

Query: 78  QMKYLMKFINEVLRRNTVAPTNFH----------YTVPKGWTVQIWGVAVHMDSRIYSNP 137
            M Y  +FINE  R   + P  F           YT+P GW V +   AVH++  +Y +P
Sbjct: 337 SMTYTFQFINETARLANIVPAIFRKALRDIKFKDYTIPAGWAVMVCPPAVHLNPEMYKDP 396

Query: 138 QEFDPSRWD--NYTPKPGEFIPFGLGSRFCPGSELTKLEITILLHHFILNYKMELVNQNC 192
             F+PSRW+    T     F+ FG G RFC G++ TKL++   LH  +  Y+ E + +  
Sbjct: 397 LVFNPSRWEGSKVTNASKHFMAFGGGMRFCVGTDFTKLQMAAFLHSLVTKYRWEEI-KGG 456