MELO3C029085.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C029085.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
DescriptionDefensin-like protein 3
Locationchr01: 20065174 .. 20065701 (-)
RNA-Seq ExpressionMELO3C029085.jh1
SyntenyMELO3C029085.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDSstart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
AACATTAAAATGAAAGGAAATGAGGTAGTCACAATATATAAAAGGGGTATTGACTCCTAAGCAGGGCATTGGAATTAAAGTACAAGAAACTGAAAGATTAAGAAGAAAAAGACATGGCAGGACGATGGAAAAAACTCACATTTGTGGCATTTGTCTTTCTATCCATTGCTTCTGGTACTCAATCTGATGCCATTATTTCATGTCATTTCCAAAATAAAAATAGAGATTAACAGAATCACGTAGTGTTGTTGTTACTTACAGGATGGATGATGGAGGAGACGGAAGGAAAGATTTGCTTCAAGCAGAGCCCGTCGTTCAAGGGAAAGTGTACTTCAAATGATGAATGTGTTAAAGCTTGCGATATCGACATGTGGTATAATGGAGTTTGCTCGAACGGTTTATGCATTTGTACCGTCATTTGTTGACTTTGTCATGGTCGTGTTATACAATTTTAATCTGGTATAAAATTGACTGAAGTTGTTATCTTCGAAATGAATAAATAAGTTGTTGATCGCCGCTGTTAAACTA

mRNA sequence

AACATTAAAATGAAAGGAAATGAGGTAGTCACAATATATAAAAGGGGTATTGACTCCTAAGCAGGGCATTGGAATTAAAGTACAAGAAACTGAAAGATTAAGAAGAAAAAGACATGGCAGGACGATGGAAAAAACTCACATTTGTGGCATTTGTCTTTCTATCCATTGCTTCTGGATGGATGATGGAGGAGACGGAAGGAAAGATTTGCTTCAAGCAGAGCCCGTCGTTCAAGGGAAAGTGTACTTCAAATGATGAATGTGTTAAAGCTTGCGATATCGACATGTGGTATAATGGAGTTTGCTCGAACGGTTTATGCATTTGTACCGTCATTTGTTGACTTTGTCATGGTCGTGTTATACAATTTTAATCTGGTATAAAATTGACTGAAGTTGTTATCTTCGAAATGAATAAATAAGTTGTTGATCGCCGCTGTTAAACTA

Coding sequence (CDS)

ATGGCAGGACGATGGAAAAAACTCACATTTGTGGCATTTGTCTTTCTATCCATTGCTTCTGGATGGATGATGGAGGAGACGGAAGGAAAGATTTGCTTCAAGCAGAGCCCGTCGTTCAAGGGAAAGTGTACTTCAAATGATGAATGTGTTAAAGCTTGCGATATCGACATGTGGTATAATGGAGTTTGCTCGAACGGTTTATGCATTTGTACCGTCATTTGTTGA

Protein sequence

MAGRWKKLTFVAFVFLSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWYNGVCSNGLCICTVIC
Homology
BLAST of MELO3C029085.jh1 vs. NCBI nr
Match: KAA0062794.1 (defensin-like protein 3 [Cucumis melo var. makuwa] >TYJ96759.1 defensin-like protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 160 bits (404), Expect = 1.64e-49
Identity = 74/74 (100.00%), Postives = 74/74 (100.00%), Query Frame = 0

Query: 1  MAGRWKKLTFVAFVFLSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWYN 60
          MAGRWKKLTFVAFVFLSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWYN
Sbjct: 1  MAGRWKKLTFVAFVFLSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWYN 60

Query: 61 GVCSNGLCICTVIC 74
          GVCSNGLCICTVIC
Sbjct: 61 GVCSNGLCICTVIC 74

BLAST of MELO3C029085.jh1 vs. NCBI nr
Match: KGN44293.1 (hypothetical protein Csa_016568 [Cucumis sativus])

HSP 1 Score: 134 bits (338), Expect = 2.00e-39
Identity = 64/75 (85.33%), Postives = 67/75 (89.33%), Query Frame = 0

Query: 1  MAGRWKKLTFVAFVFL-SIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWY 60
          MAG WKKLTFVAF+FL +IASGWMM ET GKICFKQS SFKGKC SNDECVKACD DMWY
Sbjct: 1  MAGGWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWY 60

Query: 61 NGVCSNGLCICTVIC 74
          +G CSNGLCICTVIC
Sbjct: 61 DGFCSNGLCICTVIC 75

BLAST of MELO3C029085.jh1 vs. NCBI nr
Match: XP_038887289.1 (defensin-like protein 2 [Benincasa hispida])

HSP 1 Score: 122 bits (307), Expect = 1.07e-34
Identity = 56/75 (74.67%), Postives = 64/75 (85.33%), Query Frame = 0

Query: 1  MAGR-WKKLTFVAFVFLSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWY 60
          MAG  W+KLT V F+FL++ASGWM+ ETEGK CFKQSPSF+G+CTSN+ECV ACD DMWY
Sbjct: 1  MAGEGWRKLTVVLFIFLALASGWMVVETEGKTCFKQSPSFRGQCTSNNECVGACDNDMWY 60

Query: 61 NGVCSNGLCICTVIC 74
          NG CSNGLCIC V C
Sbjct: 61 NGYCSNGLCICAVHC 75

BLAST of MELO3C029085.jh1 vs. NCBI nr
Match: AHA50469.1 (defensin-like protein 3 [Citrullus lanatus])

HSP 1 Score: 91.7 bits (226), Expect = 2.12e-22
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 0

Query: 1  MAG--RWKKLTFVAFVFLSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMW 60
          MAG   W+KLT V F+FLS+ASGWM+ ETEG+ C++ SPSFKG+C+S+++C+  CD    
Sbjct: 1  MAGSEEWRKLTIVLFIFLSLASGWMVAETEGRACYRPSPSFKGRCSSDEDCIGVCD---- 60

Query: 61 YNGVCSNGLCICTVIC 74
            G C++G CICTV C
Sbjct: 61 --GFCNHGSCICTVPC 70

BLAST of MELO3C029085.jh1 vs. NCBI nr
Match: XP_031744629.1 (defensin-1-like [Cucumis sativus])

HSP 1 Score: 85.1 bits (209), Expect = 8.08e-20
Identity = 37/70 (52.86%), Postives = 50/70 (71.43%), Query Frame = 0

Query: 5  WKKLTFVAFVFLSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWYNGVCS 64
          W+KLT V F+ LS+ASGWM+ ETEG++C++ SP  KG C+SND+C+  CD      G CS
Sbjct: 6  WRKLTVVLFILLSLASGWMVAETEGRVCYRPSPRLKGVCSSNDDCIAVCD------GFCS 65

Query: 65 NGLCICTVIC 74
          +G C+C V C
Sbjct: 66 HGKCMCEVPC 69

BLAST of MELO3C029085.jh1 vs. ExPASy TrEMBL
Match: A0A5D3BCI9 (Defensin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold986G00530 PE=4 SV=1)

HSP 1 Score: 160 bits (404), Expect = 7.93e-50
Identity = 74/74 (100.00%), Postives = 74/74 (100.00%), Query Frame = 0

Query: 1  MAGRWKKLTFVAFVFLSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWYN 60
          MAGRWKKLTFVAFVFLSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWYN
Sbjct: 1  MAGRWKKLTFVAFVFLSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWYN 60

Query: 61 GVCSNGLCICTVIC 74
          GVCSNGLCICTVIC
Sbjct: 61 GVCSNGLCICTVIC 74

BLAST of MELO3C029085.jh1 vs. ExPASy TrEMBL
Match: A0A0A0K447 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G239060 PE=4 SV=1)

HSP 1 Score: 134 bits (338), Expect = 9.68e-40
Identity = 64/75 (85.33%), Postives = 67/75 (89.33%), Query Frame = 0

Query: 1  MAGRWKKLTFVAFVFL-SIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWY 60
          MAG WKKLTFVAF+FL +IASGWMM ET GKICFKQS SFKGKC SNDECVKACD DMWY
Sbjct: 1  MAGGWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWY 60

Query: 61 NGVCSNGLCICTVIC 74
          +G CSNGLCICTVIC
Sbjct: 61 DGFCSNGLCICTVIC 75

BLAST of MELO3C029085.jh1 vs. ExPASy TrEMBL
Match: W5RQN1 (Defensin-like protein 3 OS=Citrullus lanatus OX=3654 GN=PDF2.3 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.03e-22
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 0

Query: 1  MAG--RWKKLTFVAFVFLSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMW 60
          MAG   W+KLT V F+FLS+ASGWM+ ETEG+ C++ SPSFKG+C+S+++C+  CD    
Sbjct: 1  MAGSEEWRKLTIVLFIFLSLASGWMVAETEGRACYRPSPSFKGRCSSDEDCIGVCD---- 60

Query: 61 YNGVCSNGLCICTVIC 74
            G C++G CICTV C
Sbjct: 61 --GFCNHGSCICTVPC 70

BLAST of MELO3C029085.jh1 vs. ExPASy TrEMBL
Match: A0A5D3BEY4 (Defensin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold986G00520 PE=4 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 7.22e-16
Identity = 33/69 (47.83%), Postives = 47/69 (68.12%), Query Frame = 0

Query: 6  KKLTFVAFVFLSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWYNGVCSN 65
          +KLT V  + LS+ASGWM+ ETEG++CF+  P+ KG C+S+++C+  CD      G CS 
Sbjct: 7  RKLTVVLVILLSVASGWMVAETEGRVCFRPRPTLKGVCSSDEDCIAVCD------GFCSL 66

Query: 66 GLCICTVIC 74
          G C+C V C
Sbjct: 67 GKCMCAVPC 69

BLAST of MELO3C029085.jh1 vs. ExPASy TrEMBL
Match: A0A5A7V3H5 (Defensin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold986G00550 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 2.48e-13
Identity = 33/70 (47.14%), Postives = 44/70 (62.86%), Query Frame = 0

Query: 6  KKLTFVAFVFLSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDI-DMWYNGVCS 65
          +KLT V  +FL  ASGWM+ +TEG  C+K S +F+G C S +EC + C   + W +G CS
Sbjct: 7  RKLTVVVLIFLVFASGWMVVQTEGLTCYKPSTAFQGTCNSAEECGRVCFYQEGWDDGFCS 66

Query: 66 NGLCICTVIC 74
             CICT  C
Sbjct: 67 GDKCICTEKC 76

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0062794.11.64e-49100.00defensin-like protein 3 [Cucumis melo var. makuwa] >TYJ96759.1 defensin-like pro... [more]
KGN44293.12.00e-3985.33hypothetical protein Csa_016568 [Cucumis sativus][more]
XP_038887289.11.07e-3474.67defensin-like protein 2 [Benincasa hispida][more]
AHA50469.12.12e-2255.26defensin-like protein 3 [Citrullus lanatus][more]
XP_031744629.18.08e-2052.86defensin-1-like [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3BCI97.93e-50100.00Defensin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A0A0K4479.68e-4085.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G239060 PE=4 SV=1[more]
W5RQN11.03e-2255.26Defensin-like protein 3 OS=Citrullus lanatus OX=3654 GN=PDF2.3 PE=2 SV=1[more]
A0A5D3BEY47.22e-1647.83Defensin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7V3H52.48e-1347.14Defensin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF00304Gamma-thionincoord: 30..74
e-value: 5.3E-6
score: 26.6
IPR036574Knottin, scorpion toxin-like superfamilyGENE3D3.30.30.10coord: 29..74
e-value: 6.0E-8
score: 34.8
IPR036574Knottin, scorpion toxin-like superfamilySUPERFAMILY57095Scorpion toxin-likecoord: 30..74
IPR008176Defensin, plantPROSITEPS00940GAMMA_THIONINcoord: 30..53

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C029085.jh1.t1MELO3C029085.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response