MELO3C024722 (gene) Melon (DHL92) v4

BLAST of MELO3C024722 vs. NCBI nr
Match: XP_008462778.1 (PREDICTED: probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucumis melo])

HSP 1 Score: 1615.5 bits (4182), Expect = 0.0e+00
Identity = 823/823 (100.00%), Postives = 823/823 (100.00%), Query Frame = 0

Query: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60
           MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60

Query: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120
           GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA
Sbjct: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
           KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 824
           SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS
Sbjct: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 823

BLAST of MELO3C024722 vs. NCBI nr
Match: XP_004151907.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucumis sativus] >KAE8652344.1 hypothetical protein Csa_021970 [Cucumis sativus])

HSP 1 Score: 1580.8 bits (4092), Expect = 0.0e+00
Identity = 807/823 (98.06%), Postives = 811/823 (98.54%), Query Frame = 0

Query: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60
           MDTLFVPITFSELPHPLLT NTNA SQ R+GPVSRFSPLSSLIHLNRFP  FKKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60

Query: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120
           GHFRCAAEAS HR GHHHGHHHHHHGCQHHCSGDSD VELTG QKAFVRFAEAIRWTDLA
Sbjct: 61  GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLDVNDDNLPDTSDLEHRRVPVRDV+VGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGEV+PLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
           KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKAS GSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 824
           SVRALNHPSWSWKQD QNLIH+ARSKLNTTPE SSGTIQTATS
Sbjct: 781 SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS 823

BLAST of MELO3C024722 vs. NCBI nr
Match: XP_038901378.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 1526.5 bits (3951), Expect = 0.0e+00
Identity = 784/823 (95.26%), Postives = 795/823 (96.60%), Query Frame = 0

Query: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60
           MDTLFVPI+FSE PHPLLT NTNAG+Q RIGP+SRFSP SSLI LNRFPI  KK+SYPRF
Sbjct: 1   MDTLFVPISFSESPHPLLTRNTNAGAQSRIGPLSRFSPRSSLIRLNRFPIPCKKISYPRF 60

Query: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120
           GHFRCAAEASDHR    HGHHHHHHGCQHHC GDSD VELTG QKAFVRFAEAIRWTDLA
Sbjct: 61  GHFRCAAEASDHR----HGHHHHHHGCQHHCGGDSDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHLH+CCGSAALFVTAAA PYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GG+VNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGRVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMAS HTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASSHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
           KS FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVES EYFPGRGL
Sbjct: 481 KSHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESFEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKAS GSVDFITSFCKSENESR IKDAVRASTYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRASTYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPRPGV+DAIAELQ  GKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVVDAIAELQDQGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVG+VLA RASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGMVLARRASATATAVADVLLLQDS 720

Query: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           IS VPFCIAKSRQTTSL+KQNVTLAL+SILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISAVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 824
           SVRALN PSWSWKQDFQNL+HN RSKLN  PETSSGTIQTATS
Sbjct: 781 SVRALNQPSWSWKQDFQNLMHNPRSKLNPKPETSSGTIQTATS 819

BLAST of MELO3C024722 vs. NCBI nr
Match: XP_038901377.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1516.9 bits (3926), Expect = 0.0e+00
Identity = 784/837 (93.67%), Postives = 795/837 (94.98%), Query Frame = 0

Query: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60
           MDTLFVPI+FSE PHPLLT NTNAG+Q RIGP+SRFSP SSLI LNRFPI  KK+SYPRF
Sbjct: 1   MDTLFVPISFSESPHPLLTRNTNAGAQSRIGPLSRFSPRSSLIRLNRFPIPCKKISYPRF 60

Query: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120
           GHFRCAAEASDHR    HGHHHHHHGCQHHC GDSD VELTG QKAFVRFAEAIRWTDLA
Sbjct: 61  GHFRCAAEASDHR----HGHHHHHHGCQHHCGGDSDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHLH+CCGSAALFVTAAA PYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHLCCGSAALFVTAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GG+VNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGRVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMAS HTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASSHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
           KS FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVES EYFPGRGL
Sbjct: 481 KSHFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESFEYFPGRGL 540

Query: 541 IATLHGIK--------------SGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRAS 600
           IATLHGIK              SGIGGKLRKAS GSVDFITSFCKSENESR IKDAVRAS
Sbjct: 541 IATLHGIKVEGEEYLHSISILQSGIGGKLRKASLGSVDFITSFCKSENESRKIKDAVRAS 600

Query: 601 TYGSEFVHAALSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVA 660
           TYGSEFVHAALSVDQKVTLIHLEDQPRPGV+DAIAELQ  GKLRVMMLTGDHDSSAWKVA
Sbjct: 601 TYGSEFVHAALSVDQKVTLIHLEDQPRPGVVDAIAELQDQGKLRVMMLTGDHDSSAWKVA 660

Query: 661 NAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASA 720
           NAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVG+VLA RASA
Sbjct: 661 NAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGMVLARRASA 720

Query: 721 TATAVADVLLLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLT 780
           TATAVADVLLLQDSIS VPFCIAKSRQTTSL+KQNVTLAL+SILLASLPSVLGFLPLWLT
Sbjct: 721 TATAVADVLLLQDSISAVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLT 780

Query: 781 VLLHEGGTLLVCLNSVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 824
           VLLHEGGTLLVCLNSVRALN PSWSWKQDFQNL+HN RSKLN  PETSSGTIQTATS
Sbjct: 781 VLLHEGGTLLVCLNSVRALNQPSWSWKQDFQNLMHNPRSKLNPKPETSSGTIQTATS 833

BLAST of MELO3C024722 vs. NCBI nr
Match: XP_023533453.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1440.2 bits (3727), Expect = 0.0e+00
Identity = 741/823 (90.04%), Postives = 769/823 (93.44%), Query Frame = 0

Query: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60
           MDTLFVPITFS+  H LLT  TNAG++ RIGPVS FS  SS+I L R             
Sbjct: 1   MDTLFVPITFSKPIHSLLTRTTNAGARSRIGPVSCFSLRSSVIRLKR------------- 60

Query: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120
             FRCAA+ASDHR GH   HHHHHH CQHHC GD D VELTG QKAFVRFA+AIRWTDLA
Sbjct: 61  --FRCAADASDHRHGH---HHHHHHSCQHHCGGDGDGVELTGAQKAFVRFAKAIRWTDLA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHLH+CCGSAALF+TAAA PYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHLCCGSAALFITAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSM DVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMTDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLD+NDDNLPDTSDLEHR+VPVRD+EVGSYI+VGAGESVPVDCEVY GCATITVEHL
Sbjct: 241 FALVLDMNDDNLPDTSDLEHRKVPVRDIEVGSYIMVGAGESVPVDCEVYHGCATITVEHL 300

Query: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGE+KPLQIKVG+RVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEIKPLQIKVGQRVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG MVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGFMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
            S  ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGK+LPSISVES EYFPGRGL
Sbjct: 481 NSHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKNLPSISVESFEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKAS GSVDFITSFCKSENESR IK+AVRAS+YGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPR GV+DAIAELQ    LRVMMLTGDHDSSAWK+ANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRAGVLDAIAELQDQANLRVMMLTGDHDSSAWKIANAVGINEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720
           EDKLAHVKEISRE GGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREKGGGLIMVGEGINDAPALAAATVGIVLACRASATATAVADVLLLQDS 720

Query: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCIAKSRQTT+L+KQNVTLAL+SILLASLPS+LGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTALVKQNVTLALSSILLASLPSILGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 824
           S+RALNHPSWSWKQDFQNLIHNARS L+TTPE SSGTI+TA+S
Sbjct: 781 SIRALNHPSWSWKQDFQNLIHNARSSLHTTPEPSSGTIRTASS 805

BLAST of MELO3C024722 vs. ExPASy Swiss-Prot
Match: Q9M3H5 (Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HMA1 PE=2 SV=2)

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 562/751 (74.83%), Postives = 636/751 (84.69%), Query Frame = 0

Query: 71  DHRRGHHHG-----HHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLANYLRE 130
           DH   HHH      H+HHHH  QH C     + E +  QK    FA+AI W  LANYLRE
Sbjct: 66  DHHHDHHHDDEQDHHNHHHHHHQHGCCSVELKAE-SKPQKMLFGFAKAIGWVRLANYLRE 125

Query: 131 HLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDISGGKVN 190
           HLH+CC +AA+F+ AA  PYL P+P +K LQN F+ V FPLVGVSASLDAL DI+GGKVN
Sbjct: 126 HLHLCCSAAAMFLAAAVCPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAGGKVN 185

Query: 191 IHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVL 250
           IHVLMALAAFAS+FMGN LEGGLLL MFN+AHIAEE+FT +SM+DVKELKES+PD AL++
Sbjct: 186 IHVLMALAAFASVFMGNALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDSALLI 245

Query: 251 DVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVK 310
           +V++ N+P+ SDL ++ VPV  VEVGSY+LVG GE VPVDCEVYQG ATIT+EHLTGEVK
Sbjct: 246 EVHNGNVPNISDLSYKSVPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLTGEVK 305

Query: 311 PLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGE 370
           PL+ K G+RVPGGARNLDGR+IVKATK W +STL++IVQLTEEA  NKPKLQRWLDEFGE
Sbjct: 306 PLEAKAGDRVPGGARNLDGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLDEFGE 365

Query: 371 NYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIA 430
           NYSKVVVVLS+A+A +GP LFKWPF+ T   RGSVYRALGLMVAASPCALA APLAYA A
Sbjct: 366 NYSKVVVVLSLAIAFLGPFLFKWPFLSTAACRGSVYRALGLMVAASPCALAVAPLAYATA 425

Query: 431 ISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQFA 490
           ISSCARKGILLKG  VLDA+ASCHT+AFDKTGTLTTGGL  KAIEPIYGH+ GG  S   
Sbjct: 426 ISSCARKGILLKGAQVLDALASCHTIAFDKTGTLTTGGLTCKAIEPIYGHQ-GGTNSSVI 485

Query: 491 SCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGLIATLH 550
           +CCIP+CEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSI VES EYFPGRGL AT++
Sbjct: 486 TCCIPNCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSIFVESFEYFPGRGLTATVN 545

Query: 551 GIKS-GIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVDQKVT 610
           G+K+     +LRKAS GS++FITS  KSE+ES+ IKDAV AS+YG +FVHAALSVDQKVT
Sbjct: 546 GVKTVAEESRLRKASLGSIEFITSLFKSEDESKQIKDAVNASSYGKDFVHAALSVDQKVT 605

Query: 611 LIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKL 670
           LIHLEDQPRPGV   IAEL+   +LRVMMLTGDHDSSAW+VANAVGI EVY +LKPEDKL
Sbjct: 606 LIHLEDQPRPGVSGVIAELKSWARLRVMMLTGDHDSSAWRVANAVGITEVYCNLKPEDKL 665

Query: 671 AHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGV 730
            HVK I+RE GGGLIMVGEGINDAPALAAATVGIVLA RASATA AVAD+LLL+D+I+GV
Sbjct: 666 NHVKNIAREAGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADILLLRDNITGV 725

Query: 731 PFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSVRA 790
           PFC+AKSRQTTSL+KQNV LALTSI LA+LPSVLGF+PLWLTVLLHEGGTLLVCLNSVR 
Sbjct: 726 PFCVAKSRQTTSLVKQNVALALTSIFLAALPSVLGFVPLWLTVLLHEGGTLLVCLNSVRG 785

Query: 791 LNHPSWSWKQDFQNLIHNARSKLNTTPETSS 816
           LN PSWSWKQD  +LI+  RS+  T+  ++S
Sbjct: 786 LNDPSWSWKQDIVHLINKLRSQEPTSSSSNS 814

BLAST of MELO3C024722 vs. ExPASy Swiss-Prot
Match: P58414 (Probable cadmium-transporting ATPase OS=Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) OX=169963 GN=cadA PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.9e-73
Identity = 182/601 (30.28%), Postives = 317/601 (52.75%), Query Frame = 0

Query: 189 LMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVLDVN 248
           LM +A   +  +G   EG +++++F  + + E Y   ++   ++ L +  P  AL+    
Sbjct: 148 LMTIAIIGASIIGEWAEGSIVVILFAFSEVLERYSMDKARQSIRSLMDIAPKEALI---- 207

Query: 249 DDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQ 308
                   D   + + V D+++G  +++  G+ + +D  V +G + I    +TGE  P++
Sbjct: 208 ------RRDDVEQMIAVSDIQIGDIMIIKPGQKIAMDGVVIKGYSAINQSAITGESIPVE 267

Query: 309 IKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYS 368
            KV + V  G  N +G + VK TK  E++T+S+I+ L EEAQ  +   Q ++D+F + Y+
Sbjct: 268 KKVDDEVFAGTLNEEGLLEVKVTKHVEDTTISKIIHLVEEAQGERAPAQAFVDKFAKYYT 327

Query: 369 KVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA-AAPLAYAIAIS 428
             ++++++ V ++ PL F   +         VY+ L L+V   PC+L  + P++   AI 
Sbjct: 328 PTIMLIALLVVVVPPLFFGGDWDTW------VYQGLSLLVVGCPCSLVISTPVSIVSAIG 387

Query: 429 SCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQFASC 488
           + A+ G+L+KGG  L+ +     +AFDKTGTLT G  V     P   H            
Sbjct: 388 NSAKNGVLVKGGIYLEEIGGLQAIAFDKTGTLTKGKPVVTDFIPYSEH------------ 447

Query: 489 CIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSV--GKDLPSISVESTEYFPGRGLIATLH 548
                E+ +L++  A+E  + HP+  A++  ++    D  SI +++     G+G+   ++
Sbjct: 448 ---MDEQNSLSIITALETMSQHPLASAIISKAMIDNVDYKSIEIDNFSSITGKGVKGEVN 507

Query: 549 GIKSGIG-GKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVDQKVT 608
           GI   IG  KL ++S      I     S+    + K    A  +G+E           + 
Sbjct: 508 GITYYIGSSKLFESSLEKSQSI-----SQTYQSLQKQGKTAMLFGTE--------SNILA 567

Query: 609 LIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKL 668
           +I + D+ R    + IA+L  LG    +MLTGD++ +A  +   +G++++   L PEDKL
Sbjct: 568 IIAVADEVRESSKEVIAQLHKLGIAHTIMLTGDNNDTAQFIGKEIGVSDIKAELMPEDKL 627

Query: 669 AHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGV 728
            ++KE+ ++  G + M+G+G+NDAPALAA+TVGI +    + TA   ADV L+ D +  +
Sbjct: 628 TYIKEL-KQTYGKVAMIGDGVNDAPALAASTVGIAMGGAGTDTALETADVALMGDDLKKL 687

Query: 729 PFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSVRA 786
           PF +  SR+T  +IKQN+T +L   LLA L  + G+L LW+ ++   G TLLV LN +R 
Sbjct: 688 PFIVNLSRKTLKIIKQNITFSLGIKLLALLLVLPGWLTLWIAIVADMGATLLVTLNGLRL 703

BLAST of MELO3C024722 vs. ExPASy Swiss-Prot
Match: Q60048 (Probable cadmium-transporting ATPase OS=Listeria monocytogenes OX=1639 GN=cadA PE=1 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 9.4e-73
Identity = 194/680 (28.53%), Postives = 342/680 (50.29%), Query Frame = 0

Query: 110 FAEAIRWTDLANYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGV 169
           F +   +TD  +++R++  +    + LF+ A  +   +       L N     A  + G 
Sbjct: 72  FTDPEHFTDHQSFIRKNWRLLL--SGLFI-AVGYASQIMNGEDFYLTNALFIFAIFIGGY 131

Query: 170 SASLDALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMI 229
           S   +   ++   +  +  LM +A   + F+G   EG +++++F ++   E Y   ++  
Sbjct: 132 SLFKEGFKNLLKFEFTMETLMTIAIIGAAFIGEWAEGSIVVILFAVSEALERYSMDKARQ 191

Query: 230 DVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVY 289
            ++ L +  P  ALV     D          R V V D+++G  +++  G+ + +D  V 
Sbjct: 192 SIRSLMDIAPKEALVRRSGTD----------RMVHVDDIQIGDIMIIKPGQKIAMDGHVV 251

Query: 290 QGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEA 349
           +G + +    +TGE  P++  + + V  G  N +G + V  TK  E++T+S+I+ L EEA
Sbjct: 252 KGYSAVNQAAITGESIPVEKNIDDSVFAGTLNEEGLLEVAVTKRVEDTTISKIIHLVEEA 311

Query: 350 QLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVA 409
           Q  +   Q ++D F + Y+  ++V++  +A + PLLF         +   VY+ L ++V 
Sbjct: 312 QGERAPAQAFVDTFAKYYTPAIIVIAALIATVPPLLF------GGNWETWVYQGLSVLVV 371

Query: 410 ASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTG-GLVFK 469
             PCAL  + P+A   AI + A+ G+L+KGG  L+ +     +AFDKTGTLT G  +V  
Sbjct: 372 GCPCALVVSTPVAIVTAIGNAAKNGVLVKGGVYLEEIGGLKAIAFDKTGTLTKGVPVVTD 431

Query: 470 AIEPIYGHKIGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGK--DLP 529
            IE      I  +K+                + AA+E+ + HP+  A++ +   +  DL 
Sbjct: 432 YIELTEATNIQHNKNYI--------------IMAALEQLSQHPLASAIIKYGETREMDLT 491

Query: 530 SISVESTEYFPGRGLIATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRA 589
           SI+V       G+G+  T+ G    +G  +      +  F  S  +  ++ ++      A
Sbjct: 492 SINVNDFTSITGKGIRGTVDGNTYYVGSPVLFKELLASQFTDSIHRQVSDLQL--KGKTA 551

Query: 590 STYGSEFVHAALSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKV 649
             +G+          + ++++ + D+ R      I  L  LG  + +MLTGD+ ++A  +
Sbjct: 552 MLFGTN--------QKLISIVAVADEVRSSSQHVIKRLHELGIEKTIMLTGDNQATAQAI 611

Query: 650 ANAVGINEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRAS 709
              VG++E+   L P+DKL ++K++     G + MVG+GINDAPALAAATVGI +    +
Sbjct: 612 GQQVGVSEIEGELMPQDKLDYIKQLKINF-GKVAMVGDGINDAPALAAATVGIAMGGAGT 671

Query: 710 ATATAVADVLLLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWL 769
            TA   ADV L+ D +  +PF +  SR+T  +IKQN+T +L   L+A L  + G+L LW+
Sbjct: 672 DTAIETADVALMGDDLQKLPFTVKLSRKTLQIIKQNITFSLVIKLIALLLVIPGWLTLWI 707

Query: 770 TVLLHEGGTLLVCLNSVRAL 786
            ++   G TLLV LN +R +
Sbjct: 732 AIMADMGATLLVTLNGLRLM 707

BLAST of MELO3C024722 vs. ExPASy Swiss-Prot
Match: Q6GIX1 (Probable cadmium-transporting ATPase OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=cadA PE=3 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 5.1e-71
Identity = 178/605 (29.42%), Postives = 318/605 (52.56%), Query Frame = 0

Query: 185 NIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALV 244
           ++  LM +A   +  +G   E  +++V+F ++   E +   ++   ++ L +  P  ALV
Sbjct: 160 DMKTLMTVAVIGAAIIGEWAEASIVVVLFAISEALERFSMDRARQSIRSLMDIAPKEALV 219

Query: 245 LDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 304
           +    + +          + V D+ VG  ++V  GE + +D  +  G + +    +TGE 
Sbjct: 220 MRNGQEIM----------IHVDDIAVGDIMIVKPGEKIAMDGIIINGVSAVNQAAITGES 279

Query: 305 KPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG 364
            P+   V + V  G  N +G + VK TK  E++T+S+I+ L EEAQ  +   Q ++D+F 
Sbjct: 280 VPVAKTVDDEVFAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAPAQAFVDKFA 339

Query: 365 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA-AAPLAYA 424
           + Y+ +++V++  VA++ PL F         +   VY+ L ++V   PCAL  + P++  
Sbjct: 340 KYYTPIIMVIAALVAVVPPLFF------GGSWDTWVYQGLAVLVVGCPCALVISTPISIV 399

Query: 425 IAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQ 484
            AI + A+KG+L+KGG  L+ + +   +AFDKTGTLT      K +  +   K+  D+ +
Sbjct: 400 SAIGNAAKKGVLIKGGVYLEELGAIKAIAFDKTGTLT------KGVPVVTDFKVLNDQVE 459

Query: 485 FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDL--PSISVESTEYFPGRGLI 544
                    EKE  ++  A+E  + HP+  A++  +   ++    + VE      GRG+ 
Sbjct: 460 ---------EKELFSIITALEYRSQHPLASAIMKKAEQDNITYSDVRVEDFTSITGRGIQ 519

Query: 545 ATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVDQ 604
             + G    IG           DF   F   EN+ +++++  + +          +  DQ
Sbjct: 520 GNIDGTTYYIGSPRLFKELNVSDFSLEF---ENKVKVLQNQGKTA--------MIIGTDQ 579

Query: 605 KVT-LIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 664
            +  +I + D+ R    + I +L  LG  + +MLTGD+  +A  +   VG++++   L P
Sbjct: 580 TILGVIAVADEVRETSKNVIQKLHQLGIKQTIMLTGDNQGTAEAIGAHVGVSDIQSELMP 639

Query: 665 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 724
           +DKL ++K++  E  G + M+G+G+NDAPALAA+TVGI +    + TA   AD+ L+ D 
Sbjct: 640 QDKLDYIKKMKAE-HGNVAMIGDGVNDAPALAASTVGIAMGGAGTDTAIETADIALMGDD 699

Query: 725 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 784
           +S +PF +  SR+T ++IK N+T A+   ++A L  + G+L LW+ +L   G T+LV LN
Sbjct: 700 LSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMGATILVALN 721

Query: 785 SVRAL 786
           S+R +
Sbjct: 760 SLRLM 721

BLAST of MELO3C024722 vs. ExPASy Swiss-Prot
Match: P20021 (Cadmium-transporting ATPase OS=Staphylococcus aureus OX=1280 GN=cadA PE=1 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 1.1e-70
Identity = 180/605 (29.75%), Postives = 319/605 (52.73%), Query Frame = 0

Query: 185 NIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALV 244
           ++  LM +A   +  +G   E  +++++F ++   E +   +S   ++ L +  P  ALV
Sbjct: 161 DMKTLMTVAVIGATIIGKWAEASIVVILFAISEALERFSMDRSRQSIRSLMDIAPKEALV 220

Query: 245 LDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEV 304
                + +          + V D+ VG  ++V  GE + +D  +  G + +    +TGE 
Sbjct: 221 RRNGQEII----------IHVDDIAVGDIMIVKPGEKIAMDGIIVNGLSAVNQAAITGES 280

Query: 305 KPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFG 364
            P+   V + V  G  N +G I VK TK  E++T+++I+ L EEAQ  +   Q ++D+F 
Sbjct: 281 VPVSKAVDDEVFAGTLNEEGLIEVKITKYVEDTTITKIIHLVEEAQGERAPAQAFVDKFA 340

Query: 365 ENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALA-AAPLAYA 424
           + Y+ +++V++  VA++ PL F         +   VY+ L ++V   PCAL  + P++  
Sbjct: 341 KYYTPIIMVIAALVAVVPPLFF------GGSWDTWVYQGLAVLVVGCPCALVISTPISIV 400

Query: 425 IAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQ 484
            AI + A+KG+L+KGG  L+ + +  TVAFDKTGTLT      K +  +   ++  D+ +
Sbjct: 401 SAIGNAAKKGVLVKGGVYLEKLGAIKTVAFDKTGTLT------KGVPVVTDFEVLNDQVE 460

Query: 485 FASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLP--SISVESTEYFPGRGLI 544
                    EKE  ++  A+E  + HP+  A++  +   ++P  ++ VE      GRG+ 
Sbjct: 461 ---------EKELFSIITALEYRSQHPLASAIMKKAEQDNIPYSNVQVEEFTSITGRGIK 520

Query: 545 ATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVR-ASTYGSEFVHAALSVD 604
             ++G    IG           DF   F   EN  +++++  + A   G+E         
Sbjct: 521 GIVNGTTYYIGSPKLFKELNVSDFSLGF---ENNVKILQNQGKTAMIIGTE--------K 580

Query: 605 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 664
             + +I + D+ R    + I +L  LG  + +MLTGD+  +A  +   VG++++   L P
Sbjct: 581 TILGVIAVADEVRETSKNVIQKLHQLGIKQTIMLTGDNQGTANAIGTHVGVSDIQSELMP 640

Query: 665 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 724
           +DKL ++K++  E    + M+G+G+NDAPALAA+TVGI +    + TA   AD+ L+ D 
Sbjct: 641 QDKLDYIKKMQSEY-DNVAMIGDGVNDAPALAASTVGIAMGGAGTDTAIETADIALMGDD 700

Query: 725 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 784
           +S +PF +  SR+T ++IK N+T A+   ++A L  + G+L LW+ +L   G T+LV LN
Sbjct: 701 LSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMGATILVALN 722

Query: 785 SVRAL 786
           S+R +
Sbjct: 761 SLRLM 722

BLAST of MELO3C024722 vs. ExPASy TrEMBL
Match: A0A1S3CI84 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501066 PE=3 SV=1)

HSP 1 Score: 1615.5 bits (4182), Expect = 0.0e+00
Identity = 823/823 (100.00%), Postives = 823/823 (100.00%), Query Frame = 0

Query: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60
           MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60

Query: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120
           GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA
Sbjct: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
           KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 824
           SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS
Sbjct: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 823

BLAST of MELO3C024722 vs. ExPASy TrEMBL
Match: A0A0A0LN89 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G433360 PE=3 SV=1)

HSP 1 Score: 1560.8 bits (4040), Expect = 0.0e+00
Identity = 801/823 (97.33%), Postives = 805/823 (97.81%), Query Frame = 0

Query: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60
           MDTLFVPITFSELPHPLLT NTNA SQ R+GPVSRFSPLSSLIHLNRFP  FKKLSYPRF
Sbjct: 1   MDTLFVPITFSELPHPLLTCNTNAASQSRVGPVSRFSPLSSLIHLNRFPTRFKKLSYPRF 60

Query: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120
           GHFRCAAEAS HR GHHHGHHHHHHGCQHHCSGDSD VELTG QKAFVRFAEAIRWTDLA
Sbjct: 61  GHFRCAAEASGHRHGHHHGHHHHHHGCQHHCSGDSDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLDVNDDNLPDTSDLEHRRVPVRDV+VGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVKVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGEV+PLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVRPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
           KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL
Sbjct: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKAS GSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720

Query: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLG     L VLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLG-----LKVLLHEGGTLLVCLN 780

Query: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 824
           SVRALNHPSWSWKQD QNLIH+ARSKLNTTPE SSGTIQTATS
Sbjct: 781 SVRALNHPSWSWKQDLQNLIHDARSKLNTTPENSSGTIQTATS 818

BLAST of MELO3C024722 vs. ExPASy TrEMBL
Match: A0A6J1L1A5 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499516 PE=3 SV=1)

HSP 1 Score: 1438.3 bits (3722), Expect = 0.0e+00
Identity = 740/823 (89.91%), Postives = 769/823 (93.44%), Query Frame = 0

Query: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60
           MDTLF+PITFS+  H LLT  TNAG++ RIGPVS FS  SS I L R             
Sbjct: 1   MDTLFIPITFSKPIHSLLTRTTNAGARSRIGPVSCFSLRSSAIRLKR------------- 60

Query: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120
             FRCAA+ASDHR GHH  HHHHHH CQHHC GD D VELTG QKAFV FAEAIRWTDLA
Sbjct: 61  --FRCAADASDHRHGHH--HHHHHHSCQHHCGGDGDGVELTGAQKAFVGFAEAIRWTDLA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHLH+CCGSAALF+TAAA PYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHLCCGSAALFITAAACPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSM DVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMTDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLD+NDDNLPDTSDLEHR+VPVRD+EVGSYI+VGAGESVPVDCEVY GCATITVEHL
Sbjct: 241 FALVLDMNDDNLPDTSDLEHRKVPVRDIEVGSYIMVGAGESVPVDCEVYHGCATITVEHL 300

Query: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGE+KPLQIKVG+RVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQ NKPKLQRWL
Sbjct: 301 TGEIKPLQIKVGQRVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQQNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG MVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGFMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
           KS  ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGK+LPSISVES EYFPGRGL
Sbjct: 481 KSHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKNLPSISVESFEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKAS GSVDFITSFCKSENESR IK+AVRAS++GSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSFGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPR GV+DAIAELQ   KLRVMMLTGDHDSSAWK+ANAVGI+EVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRAGVLDAIAELQDQAKLRVMMLTGDHDSSAWKIANAVGISEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720
           EDKLAHVKEISRE GGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREKGGGLIMVGEGINDAPALAAATVGIVLACRASATATAVADVLLLQDS 720

Query: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCIAKSRQTT+L+KQNVTLAL+SILLASLPS+LGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTALVKQNVTLALSSILLASLPSILGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 824
           S+RALNHPSWSWKQDFQNLIHNARS L+TTPE SSGTI+TA+S
Sbjct: 781 SIRALNHPSWSWKQDFQNLIHNARSSLHTTPEPSSGTIRTASS 806

BLAST of MELO3C024722 vs. ExPASy TrEMBL
Match: A0A6J1G6Z6 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111451327 PE=3 SV=1)

HSP 1 Score: 1431.8 bits (3705), Expect = 0.0e+00
Identity = 739/823 (89.79%), Postives = 767/823 (93.20%), Query Frame = 0

Query: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60
           MDTLFVPITFS+  H LLT  TNAG++ RIGPVS FS  SS+I L R             
Sbjct: 1   MDTLFVPITFSKPIHSLLTRTTNAGARSRIGPVSCFSLRSSVIRLKR------------- 60

Query: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120
             FRCAA+ASDHR    HGHHHHHH CQHHC GD D VELTG QKAFVRFAEAIRWTDLA
Sbjct: 61  --FRCAADASDHR----HGHHHHHHSCQHHCGGDGDGVELTGAQKAFVRFAEAIRWTDLA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHLH+CCGSAALF+TAAA PYLVPKPAVK LQNVFIAVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLHLCCGSAALFITAAACPYLVPKPAVKLLQNVFIAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSM DVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMTDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLD+NDDNLPDTSDLEHR+VPVRD+EVGSYI+VGAGESVPVDCEVY GCATITVEHL
Sbjct: 241 FALVLDMNDDNLPDTSDLEHRKVPVRDIEVGSYIMVGAGESVPVDCEVYHGCATITVEHL 300

Query: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGE+KPLQIKVG+RVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEIKPLQIKVGQRVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALG MVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGFMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMASC TVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCDTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
            S  ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGK+LPSISVES EYFPGRGL
Sbjct: 481 NSHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKNLPSISVESFEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600
           IATLHGIKSGIGG LRKAS GSVDFITSFCKSENESR IK+AVRAS+YGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGPLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPR GV+DAIAELQ   KLRVMMLTGDHDSSAWK+ANAVGI+EVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRAGVLDAIAELQDQAKLRVMMLTGDHDSSAWKIANAVGIDEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720
           EDKLAHVKEISRE GGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQDS
Sbjct: 661 EDKLAHVKEISREKGGGLIMVGEGINDAPALAAATVGIVLACRASATATAVADVLLLQDS 720

Query: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCIAKSRQTT+L+KQNVTLAL+SILLASLPS+LGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCIAKSRQTTALVKQNVTLALSSILLASLPSILGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTATS 824
           S+RALNHPSWSWKQDFQNLIHNARS L+TTPE SSGTI+TA+S
Sbjct: 781 SIRALNHPSWSWKQDFQNLIHNARSSLHTTPEPSSGTIRTASS 804

BLAST of MELO3C024722 vs. ExPASy TrEMBL
Match: A0A6J1DBA2 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018894 PE=3 SV=1)

HSP 1 Score: 1375.9 bits (3560), Expect = 0.0e+00
Identity = 715/822 (86.98%), Postives = 750/822 (91.24%), Query Frame = 0

Query: 1   MDTLFVPITFSELPHPLLTSNTNAGSQPRIGPVSRFSPLSSLIHLNRFPICFKKLSYPRF 60
           MD LFV        H  +   TN G+     PV RFS  SSLI +    +  K   YPR 
Sbjct: 1   MDALFV-------HHSYIRRTTNDGAHSTAIPVPRFSLRSSLIGIKLLHLHSKNFPYPRL 60

Query: 61  GHFRCAAEASDHRRGHHHGHHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLA 120
               CA E +    GHHHG   HHHGCQHHC GD   VE TG QKAF+RFA+AIRWT+LA
Sbjct: 61  RRIPCAPEGA----GHHHG---HHHGCQHHCDGDG--VEPTGAQKAFIRFAKAIRWTELA 120

Query: 121 NYLREHLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDIS 180
           NYLREHL +CCGSAALF+TAAA PYLVPKPAVKPLQNV +AVAFPLVGVSASLDALTDIS
Sbjct: 121 NYLREHLQLCCGSAALFLTAAACPYLVPKPAVKPLQNVLVAVAFPLVGVSASLDALTDIS 180

Query: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPD 240
           GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFN+AHIAEEYFTKQSMIDVKELKESHPD
Sbjct: 181 GGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNLAHIAEEYFTKQSMIDVKELKESHPD 240

Query: 241 FALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300
           FALVLD+NDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL
Sbjct: 241 FALVLDMNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHL 300

Query: 301 TGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360
           TGEVKPL+IKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL
Sbjct: 301 TGEVKPLEIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWL 360

Query: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420
           DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL
Sbjct: 361 DEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGD 480
           AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPI+GHK+GG+
Sbjct: 421 AYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIFGHKVGGE 480

Query: 481 KSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGL 540
           K+  ASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHS GKDLPSISVES EYFPGRGL
Sbjct: 481 KTHLASCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSAGKDLPSISVESFEYFPGRGL 540

Query: 541 IATLHGIKSGIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVD 600
           IATLHGIKSGIGGKLRKAS GSVDFITSFCKSENESR IK+AVRAS+YGSEFVHAALSVD
Sbjct: 541 IATLHGIKSGIGGKLRKASLGSVDFITSFCKSENESRKIKEAVRASSYGSEFVHAALSVD 600

Query: 601 QKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660
           QKVTLIHLEDQPRPG+++AI+ELQ   K+RVMMLTGDHDSSAWKVANAVGINEVYYSLKP
Sbjct: 601 QKVTLIHLEDQPRPGMLNAISELQDHAKVRVMMLTGDHDSSAWKVANAVGINEVYYSLKP 660

Query: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDS 720
           EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLA RASATATAVADVLLLQD+
Sbjct: 661 EDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLARRASATATAVADVLLLQDN 720

Query: 721 ISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780
           IS VPFCIAKSRQTTSL+KQNVTLAL+SILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISCVPFCIAKSRQTTSLVKQNVTLALSSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SVRALNHPSWSWKQDFQNLIHNARSKLNTTPETSSGTIQTAT 823
           S+RALN PSWSWKQDFQNL+ +ARS++  T +TSSGTIQTA+
Sbjct: 781 SIRALNQPSWSWKQDFQNLVRDARSRVLATRQTSSGTIQTAS 806

BLAST of MELO3C024722 vs. TAIR 10
Match: AT4G37270.1 (heavy metal atpase 1 )

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 562/751 (74.83%), Postives = 636/751 (84.69%), Query Frame = 0

Query: 71  DHRRGHHHG-----HHHHHHGCQHHCSGDSDRVELTGTQKAFVRFAEAIRWTDLANYLRE 130
           DH   HHH      H+HHHH  QH C     + E +  QK    FA+AI W  LANYLRE
Sbjct: 66  DHHHDHHHDDEQDHHNHHHHHHQHGCCSVELKAE-SKPQKMLFGFAKAIGWVRLANYLRE 125

Query: 131 HLHMCCGSAALFVTAAAFPYLVPKPAVKPLQNVFIAVAFPLVGVSASLDALTDISGGKVN 190
           HLH+CC +AA+F+ AA  PYL P+P +K LQN F+ V FPLVGVSASLDAL DI+GGKVN
Sbjct: 126 HLHLCCSAAAMFLAAAVCPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAGGKVN 185

Query: 191 IHVLMALAAFASIFMGNPLEGGLLLVMFNMAHIAEEYFTKQSMIDVKELKESHPDFALVL 250
           IHVLMALAAFAS+FMGN LEGGLLL MFN+AHIAEE+FT +SM+DVKELKES+PD AL++
Sbjct: 186 IHVLMALAAFASVFMGNALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDSALLI 245

Query: 251 DVNDDNLPDTSDLEHRRVPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVK 310
           +V++ N+P+ SDL ++ VPV  VEVGSY+LVG GE VPVDCEVYQG ATIT+EHLTGEVK
Sbjct: 246 EVHNGNVPNISDLSYKSVPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLTGEVK 305

Query: 311 PLQIKVGERVPGGARNLDGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGE 370
           PL+ K G+RVPGGARNLDGR+IVKATK W +STL++IVQLTEEA  NKPKLQRWLDEFGE
Sbjct: 306 PLEAKAGDRVPGGARNLDGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLDEFGE 365

Query: 371 NYSKVVVVLSVAVALIGPLLFKWPFICTPGFRGSVYRALGLMVAASPCALAAAPLAYAIA 430
           NYSKVVVVLS+A+A +GP LFKWPF+ T   RGSVYRALGLMVAASPCALA APLAYA A
Sbjct: 366 NYSKVVVVLSLAIAFLGPFLFKWPFLSTAACRGSVYRALGLMVAASPCALAVAPLAYATA 425

Query: 431 ISSCARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQFA 490
           ISSCARKGILLKG  VLDA+ASCHT+AFDKTGTLTTGGL  KAIEPIYGH+ GG  S   
Sbjct: 426 ISSCARKGILLKGAQVLDALASCHTIAFDKTGTLTTGGLTCKAIEPIYGHQ-GGTNSSVI 485

Query: 491 SCCIPSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGLIATLH 550
           +CCIP+CEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSI VES EYFPGRGL AT++
Sbjct: 486 TCCIPNCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSIFVESFEYFPGRGLTATVN 545

Query: 551 GIKS-GIGGKLRKASFGSVDFITSFCKSENESRMIKDAVRASTYGSEFVHAALSVDQKVT 610
           G+K+     +LRKAS GS++FITS  KSE+ES+ IKDAV AS+YG +FVHAALSVDQKVT
Sbjct: 546 GVKTVAEESRLRKASLGSIEFITSLFKSEDESKQIKDAVNASSYGKDFVHAALSVDQKVT 605

Query: 611 LIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGINEVYYSLKPEDKL 670
           LIHLEDQPRPGV   IAEL+   +LRVMMLTGDHDSSAW+VANAVGI EVY +LKPEDKL
Sbjct: 606 LIHLEDQPRPGVSGVIAELKSWARLRVMMLTGDHDSSAWRVANAVGITEVYCNLKPEDKL 665

Query: 671 AHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATAVADVLLLQDSISGV 730
            HVK I+RE GGGLIMVGEGINDAPALAAATVGIVLA RASATA AVAD+LLL+D+I+GV
Sbjct: 666 NHVKNIAREAGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADILLLRDNITGV 725

Query: 731 PFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFLPLWLTVLLHEGGTLLVCLNSVRA 790
           PFC+AKSRQTTSL+KQNV LALTSI LA+LPSVLGF+PLWLTVLLHEGGTLLVCLNSVR 
Sbjct: 726 PFCVAKSRQTTSLVKQNVALALTSIFLAALPSVLGFVPLWLTVLLHEGGTLLVCLNSVRG 785

Query: 791 LNHPSWSWKQDFQNLIHNARSKLNTTPETSS 816
           LN PSWSWKQD  +LI+  RS+  T+  ++S
Sbjct: 786 LNDPSWSWKQDIVHLINKLRSQEPTSSSSNS 814

BLAST of MELO3C024722 vs. TAIR 10
Match: AT4G30110.1 (heavy metal atpase 2 )

HSP 1 Score: 211.5 bits (537), Expect = 2.6e-54
Identity = 178/634 (28.08%), Postives = 310/634 (48.90%), Query Frame = 0

Query: 159 FIAVAFPLVGVSASL-DALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAH 218
           ++AVA  + G+   L  A+  ++  +++I++L+ +   A+I M +  E  +++ +F +A 
Sbjct: 112 WLAVAAVVAGIYPILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAE 171

Query: 219 IAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVG 278
             +   + ++   ++ L    P  A++ +  ++            V V +++  + I V 
Sbjct: 172 WLQSRASYKASAVMQSLMSLAPQKAVIAETGEE------------VEVDELKTNTVIAVK 231

Query: 279 AGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEES 338
           AGE++P+D  V  G   +  + LTGE  P+       V  G  NL+G I V  T   E+ 
Sbjct: 232 AGETIPIDGVVVDGNCEVDEKTLTGEAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDC 291

Query: 339 TLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFR 398
            ++++ +L EEAQ +K + QR++D+  + Y+  ++++S+    I P   K         +
Sbjct: 292 VVAKMAKLVEEAQNSKTETQRFIDKCSKYYTPAIILISICFVAI-PFALK-----VHNLK 351

Query: 399 GSVYRALGLMVAASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKT 458
             V+ AL ++V+A PC L  + P+A   A++  A  G+L+KG   L+ +A    VAFDKT
Sbjct: 352 HWVHLALVVLVSACPCGLILSTPVATFCALTKAATSGLLIKGADYLETLAKIKIVAFDKT 411

Query: 459 GTLTTGGLVFKAIEPIYGHKIGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVV 518
           GT+T G  +                  F S       +  L   ++ E  ++HP+  AVV
Sbjct: 412 GTITRGEFIV---------------MDFQSLSEDISLQSLLYWVSSTESKSSHPMAAAVV 471

Query: 519 DH--SVGKDLPSISVESTEYFPGRGLIATLHGIKSGIGGKLRKASFGSVDFITSFCKSEN 578
           D+  SV  +    +VE  + FPG G+   + G +  IG K   +  G        C S  
Sbjct: 472 DYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKRIASRAG--------CLSVP 531

Query: 579 ESRMIKDAVRASTYGSEFVHAALSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMML 638
           +  +  D     T G  +V   L+      + +L D  R GV  A+ EL+ LG +++ ML
Sbjct: 532 DIDV--DTKGGKTIGYVYVGETLA-----GVFNLSDACRSGVAQAMKELKSLG-IKIAML 591

Query: 639 TGDHDSSAWKVANAVG--INEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALA 698
           TGD+ ++A      +G  ++ V   L PEDK   +K++ RE  G   MVG+G+NDAPALA
Sbjct: 592 TGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKRE-EGPTAMVGDGLNDAPALA 651

Query: 699 AATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLA 758
            A +GI +    SA AT   +++L+ + I  +P  I  +++    + +NV +++T     
Sbjct: 652 TADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKGAI 695

Query: 759 SLPSVLGFLPLWLTVLLHEGGTLLVCLNSVRALN 787
              +  G   +W  VL   G  LLV LNS+  L+
Sbjct: 712 LALAFAGHPLIWAAVLADVGTCLLVILNSMLLLS 695

BLAST of MELO3C024722 vs. TAIR 10
Match: AT2G19110.1 (heavy metal atpase 4 )

HSP 1 Score: 210.7 bits (535), Expect = 4.5e-54
Identity = 176/635 (27.72%), Postives = 308/635 (48.50%), Query Frame = 0

Query: 159 FIAVAFPLVGVSASL-DALTDISGGKVNIHVLMALAAFASIFMGNPLEGGLLLVMFNMAH 218
           ++AVA    G+   L  A   I   +++I++L+ +   A++ M + +E   ++ +F ++ 
Sbjct: 122 WLAVAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISD 181

Query: 219 IAEEYFTKQSMIDVKELKESHPDFALVLDVNDDNLPDTSDLEHRRVPVRDVEVGSYILVG 278
             E   + ++   ++ L    P  A++ +  ++            V V +V+V + + V 
Sbjct: 182 WLETRASYKATSVMQSLMSLAPQKAIIAETGEE------------VEVDEVKVDTVVAVK 241

Query: 279 AGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNLDGRIIVKATKTWEES 338
           AGE++P+D  V  G   +  + LTGE  P+  +    V  G  NL+G I VK T    + 
Sbjct: 242 AGETIPIDGIVVDGNCEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDC 301

Query: 339 TLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAVALIGPLLFKWPFICTPGFR 398
            ++++ +L EEAQ +K K QR +D+  + Y+  ++++S  VA++ P++ K         +
Sbjct: 302 VVAKMAKLVEEAQSSKTKSQRLIDKCSQYYTPAIILVSACVAIV-PVIMK-----VHNLK 361

Query: 399 GSVYRALGLMVAASPCAL-AAAPLAYAIAISSCARKGILLKGGHVLDAMASCHTVAFDKT 458
              + AL ++V+  PC L  + P+A   A++  A  G+L+K    LD ++    VAFDKT
Sbjct: 362 HWFHLALVVLVSGCPCGLILSTPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKT 421

Query: 459 GTLTTGGLVFKAIEPIYGHKIGGDKSQFASCCIPSCEKEALAVAAAMEKGTTHPIGRAVV 518
           GT+T G  +                  F S       +  L   +++E  ++HP+   +V
Sbjct: 422 GTITRGEFIV---------------IDFKSLSRDINLRSLLYWVSSVESKSSHPMAATIV 481

Query: 519 DH--SVGKDLPSISVESTEYFPGRGLIATLHGIKSGIGGKLRKASFGSVDFITSFCKSEN 578
           D+  SV  +     VE  + FPG G+   + G    IG K   +  G        C +  
Sbjct: 482 DYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFIGNKKIASRAG--------CSTVP 541

Query: 579 ESRMIKDAVRASTYGSEFVHAALSVDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMML 638
           E       +   T G + V      ++     +L D  R GV  A+AEL+ LG ++  ML
Sbjct: 542 E-------IEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKSLG-IKTAML 601

Query: 639 TGDHDSSAWKVANAVG--INEVYYSLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALA 698
           TGD+ ++A      +G  ++ V+  L PEDK   ++E  +E  G   MVG+G+NDAPALA
Sbjct: 602 TGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKE--GPTAMVGDGVNDAPALA 661

Query: 699 AATVGIVLAHRASATATAVADVLLLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLA 758
            A +GI +    SA AT   +++L+ + I  +P  +  +R+    + +NV L++  IL A
Sbjct: 662 TADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLSI--ILKA 703

Query: 759 SLPSV--LGFLPLWLTVLLHEGGTLLVCLNSVRAL 786
            + ++   G   +W  VL+  G  LLV  NS+  L
Sbjct: 722 GILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLL 703

BLAST of MELO3C024722 vs. TAIR 10
Match: AT5G21930.1 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 196.4 bits (498), Expect = 8.8e-50
Identity = 179/586 (30.55%), Postives = 277/586 (47.27%), Query Frame = 0

Query: 208 LLLVMFNMAHIAEEYFTKQSMIDVKELKE-SHPDFALVLDVNDDNLPDTSDLEHR----R 267
           +LL    +    EE    Q+  D+ EL         LV+  +D+N P  S L        
Sbjct: 286 MLLGFVLLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICIN 345

Query: 268 VPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNL 327
           V V D+ VG  +LV  GE+ PVD  V  G + +    LTGE  P+  + G  V  G  N 
Sbjct: 346 VSVDDIRVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINW 405

Query: 328 DGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAV---- 387
           DG + +KA+ T   ST+S+IV++ E+AQ N   +QR  D     +   ++ LS       
Sbjct: 406 DGPLRIKASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAFW 465

Query: 388 ALIGPLLFKWPFICTPGFRG--------SVYRALGLMVAASPCALA-AAPLAYAIAISSC 447
             +G  +F  P +      G        S+  A+ ++V + PCAL  A P A  I  S  
Sbjct: 466 YYVGSHIF--PDVLLNDIAGPDGDALALSLKLAVDVLVVSCPCALGLATPTAILIGTSLG 525

Query: 448 ARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQFASCCI 507
           A++G L++GG VL+ +AS   VA DKTGTLT G  V   +  + G++             
Sbjct: 526 AKRGYLIRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYE------------- 585

Query: 508 PSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGLIATLHGIKS 567
              E+E L +AAA+EK  THPI +A+V+ +   +L +         PG G +A + G   
Sbjct: 586 ---EQEVLKMAAAVEKTATHPIAKAIVNEAESLNLKTPETRGQLTEPGFGTLAEIDG--- 645

Query: 568 GIGGKLRKASFGSVDFITS-FCKSENESRMIK----------DAVRASTYGSEFVHAALS 627
                 R  + GS+++++  F K  + S M+K          +    S Y    V+    
Sbjct: 646 ------RFVAVGSLEWVSDRFLKKNDSSDMVKLESLLDHKLSNTSSTSRYSKTVVYVGRE 705

Query: 628 VDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGI--NEVYY 687
            +  +  I + D  R      +A LQ  G ++ ++L+GD + +   VA  VGI      Y
Sbjct: 706 GEGIIGAIAISDCLRQDAEFTVARLQEKG-IKTVLLSGDREGAVATVAKNVGIKSESTNY 765

Query: 688 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATA-VADVL 747
           SL PE K   +  + +  G  + MVG+GINDAP+LA A VGI L   A   A +  A V+
Sbjct: 766 SLSPEKKFEFISNL-QSSGHRVAMVGDGINDAPSLAQADVGIALKIEAQENAASNAASVI 825

Query: 748 LLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFL 762
           L+++ +S V   ++ ++ T S + QN+  A+   ++ S+P   G L
Sbjct: 826 LVRNKLSHVVDALSLAQATMSKVYQNLAWAIAYNVI-SIPIAAGVL 840

BLAST of MELO3C024722 vs. TAIR 10
Match: AT5G21930.2 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 196.4 bits (498), Expect = 8.8e-50
Identity = 179/586 (30.55%), Postives = 277/586 (47.27%), Query Frame = 0

Query: 208 LLLVMFNMAHIAEEYFTKQSMIDVKELKE-SHPDFALVLDVNDDNLPDTSDLEHR----R 267
           +LL    +    EE    Q+  D+ EL         LV+  +D+N P  S L        
Sbjct: 286 MLLGFVLLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICIN 345

Query: 268 VPVRDVEVGSYILVGAGESVPVDCEVYQGCATITVEHLTGEVKPLQIKVGERVPGGARNL 327
           V V D+ VG  +LV  GE+ PVD  V  G + +    LTGE  P+  + G  V  G  N 
Sbjct: 346 VSVDDIRVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINW 405

Query: 328 DGRIIVKATKTWEESTLSRIVQLTEEAQLNKPKLQRWLDEFGENYSKVVVVLSVAV---- 387
           DG + +KA+ T   ST+S+IV++ E+AQ N   +QR  D     +   ++ LS       
Sbjct: 406 DGPLRIKASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAFW 465

Query: 388 ALIGPLLFKWPFICTPGFRG--------SVYRALGLMVAASPCALA-AAPLAYAIAISSC 447
             +G  +F  P +      G        S+  A+ ++V + PCAL  A P A  I  S  
Sbjct: 466 YYVGSHIF--PDVLLNDIAGPDGDALALSLKLAVDVLVVSCPCALGLATPTAILIGTSLG 525

Query: 448 ARKGILLKGGHVLDAMASCHTVAFDKTGTLTTGGLVFKAIEPIYGHKIGGDKSQFASCCI 507
           A++G L++GG VL+ +AS   VA DKTGTLT G  V   +  + G++             
Sbjct: 526 AKRGYLIRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYE------------- 585

Query: 508 PSCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSISVESTEYFPGRGLIATLHGIKS 567
              E+E L +AAA+EK  THPI +A+V+ +   +L +         PG G +A + G   
Sbjct: 586 ---EQEVLKMAAAVEKTATHPIAKAIVNEAESLNLKTPETRGQLTEPGFGTLAEIDG--- 645

Query: 568 GIGGKLRKASFGSVDFITS-FCKSENESRMIK----------DAVRASTYGSEFVHAALS 627
                 R  + GS+++++  F K  + S M+K          +    S Y    V+    
Sbjct: 646 ------RFVAVGSLEWVSDRFLKKNDSSDMVKLESLLDHKLSNTSSTSRYSKTVVYVGRE 705

Query: 628 VDQKVTLIHLEDQPRPGVIDAIAELQHLGKLRVMMLTGDHDSSAWKVANAVGI--NEVYY 687
            +  +  I + D  R      +A LQ  G ++ ++L+GD + +   VA  VGI      Y
Sbjct: 706 GEGIIGAIAISDCLRQDAEFTVARLQEKG-IKTVLLSGDREGAVATVAKNVGIKSESTNY 765

Query: 688 SLKPEDKLAHVKEISREMGGGLIMVGEGINDAPALAAATVGIVLAHRASATATA-VADVL 747
           SL PE K   +  + +  G  + MVG+GINDAP+LA A VGI L   A   A +  A V+
Sbjct: 766 SLSPEKKFEFISNL-QSSGHRVAMVGDGINDAPSLAQADVGIALKIEAQENAASNAASVI 825

Query: 748 LLQDSISGVPFCIAKSRQTTSLIKQNVTLALTSILLASLPSVLGFL 762
           L+++ +S V   ++ ++ T S + QN+  A+   ++ S+P   G L
Sbjct: 826 LVRNKLSHVVDALSLAQATMSKVYQNLAWAIAYNVI-SIPIAAGVL 840