MELO3C023314.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C023314.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
DescriptionBifunctional dihydrofolate reductase-thymidylate synthase
Locationchr11: 4405811 .. 4412110 (+)
RNA-Seq ExpressionMELO3C023314.jh1
SyntenyMELO3C023314.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonstart_codonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGTTCTCGAATATTCCAATTTGGATCATAGACGGCGATAATCCGCAGCAAGAAACGCGTCTTTGGATTTTCCTCTGTTTTCTTCTTCAAATCTTCTTTCCCACATTTACCCTCTTCCATTACAGACCAAAAAAAGTTAAAAAACCACAAAAAGATCGAAACCAGATAAACAAAAGAAGCGGTTTTTCCATCTAAAAAACTCCCTACCAGCTGGATTTCCCACTCTTCTTTCCTTTCCAGAAATGCCCATTTCGTAAAAACCCTTCTAAATCCATAAACGTTCACCCCCATTTCCTCTCCTAAACCTAACCCTCTTCCAGTCGAAGGTCAGCCTAAGTTTATCTTAGGCTTTTTCGAGTTGAATATTTTCATCCCCCGTTTAGCCTCGGTGGGTTGTGTTTAATTTTCTTTTTCCCCCTTCTTTTTCTTTCTGATTTTGTTGCCCCATTTTGAAATTTGATTGTGGGGGAATGGGGAACGAGCTTTTCTGGTTTCTGTTCTTGTTCTTGTTATCTGGGTTTGAGGTTAAATCTTCTTTCTGTAAATGGGTGATCGTCCATTGTTCTTATTCTTCATGCATTTTGTAGACTGGATTTGCATTTGAACTGTGAGTAGTTCACTGCATGTGCCCTTTTCAATAGTTTGTTTGTTTGGTCTGTCTGAGTTTTTCAACCTCTTGTTCGTCTCTTTATGATATTATCCATTATCTCTAATGGAAAGCGAATGTGCGTTGTAAGGATTGTGTTCCTTATTTGATTTTTCTCTGCAGTTTGTTGGGTAGGGAAGAATCTGGAGGTAAATTAAAGGAAAAAAGAAGAATGCATTACAATGCAACAATTGTTTAACACTTTTTCTTCTGTTGTTTACTTTTTAATTTTTTAGTGAATTATATTCTTGTAAAACTAAGGGTTTGGTAGTGGGTTCATTTTTTTTAGTGTTTTTCGTTTGTTTGGTAGAATAATTAATATGAATATATTAGGTAATATGTTCAGTTCTTTTGAACCAAAAGATATATCAATTGGAAAAGTAAGCCCTAGACGAAATGATCTCTAACTTTACCTAAATCCCTCTCCTTTGAATGGTGAACTCCATTTTTTTTTCTAATTTAATATCCTTTTGCCTAAAATTAGGTTAAACATTATAGAAGTAATTTGTTTTGATCTCTTTTAATTGAAGAAACATTCAGGACATGCGTCAAACTTCTGTTTGTGAAAAGATGAATTAGGAAAATCTATTTAGAAATTGATTTATTTTCTTCTTTAGTTGTTTTGGTTTTAATTCTTCACCTCCAATAATGTGAAGCAACCATTTCATTTGTTTCAAAATTTTCTAGATTTTGGTTTGCAAGTTTTATTCTTTGTCCTCTGGCATCCGTGTATGGAGAGTTATAAATCTCCATCCACATTAATTTGAGCCAATAAGTTTTTAAAGTTTATGACAAGTTTGTGATATTTTTAACGAAATTGAAGTGTTTTTGATTTCAAAGAAAGAGTATTGAATGTTTAATCATTGGATCATGTTCCAAATAATTGAATTTTTTCGGTTTTATTGAATTTCAGGTTCTACCACACTTCCGTGGAAGTTGGGTGTGTGGGGGAAAATCATTGAAGTCACCACTATCTAGGTTTGGTCAAGCTTTTAGTTCTAATATGGCCGCTGATCCTTTGACAATTTCTAATGGCATTGCCAATGTCAATTCACTACCTGATCCACGGAGGACTTATCAAGTTGTTGTAGCTGCTACTGAAAATTTGGGTATCGGTAAGGATGGGAAATTACCATGGAGGTTGCCTTCTGATCTCAAGTTTTTTAAAAACATAACCACAACAACATCAGATCCTGGGAAAAGGAATGCAATTCTAATGGGTCGTAAAACATGGGAGAGTCTTCCTGCCGAGCATCGACCACTACCTGGTCGCCTGAATGTTGTTCTGACTCGTTCTGGAAGTTTTGACATTGCAACTGTTGAAAATGTTATAATATGTGGTAGTATAATGTCTGCTTTGGAATTATTAGCAGCTTCTCCTTACTGTTTGTCAATTGAGAAGGTGTTTGTTATAGGTGGTGGTGAAATATTAAGGTGTGCACTTGTTTTCCTGACCTCCCTTAGTGTACTATTAGTTGTTTCTTTGCACTTATTGTGTTCTTGATGCAGGGAAGCTATGAATGCCCCTGAATGTGATGCTGTCCACATTACTGAAATTGAGACAAATATTGAATGTGACACCTTCATACCAGCTATTGATAAGTCGATGTTTCAACCATGGTACTCATCTTTTCCTGTGGTTGAAAATAACTTGCGATATTCGTTTAATAGTTATGTTCGTGTGAGGAGCTCAGCTGAACTTCCCATTGATCAGAACAATGGTTTGGTCTCTCATAACAAGCCTGACTTTTCTAACATTGAAGCTAAGAAGTTTTCTTTCCTACCTAAGGAGATTTTCGAGAGGCATGAGGAGCACTTGTACCTAAAACTTGTTCAAGAAATCATCTCAGATGGTATTCCTAAGGATGATAGAACAGGAACAGGTACTTTGTCAAAATTTGGTTGCCAGGTAACGTTATCTTATTATTTAAAATGTCTATTTAACTATTTTTTATACCAGCTGCTCTACTTTAAATGCTAATGTGTGTACAAGTTTCTGTGTTGTTCCTGGTATCTGTGTGCAGATGCGGTACAACTTGCGCAAGACTTTTCCACTGCTTACAACTAAGGCATGTGCTGGTTTCCTGTTTTGATTTATTTTGTAGCTGTATTATGATATCAACACTCCCTTTTCTTGTTTTATTTCCCATATTGAATTTCTAATAATCTGGTGCATCATATATGGGCATGGTTCTGTTGTTGACATATATCCTTACCGTTTGATCCTTCAGAAAGTATTCTGGCGAGGGGTGGTTGAAGAACTTCTTTGGTTCATTAGTGGTTCAACCAATGCAAAGGTAAGAATTAAGATGCTATGCTATCTCTATCACTAGCACTTTTAAGCTTTACTTTACTAGTTGGTGGCATTGTATTCCCCCCTCCTCCATGTTGTTTCGTCTATCATCTTTTCGAGTCAGAATTTCCAAGGCTTCAAAGATAAGGAAGGTTTAGGAATTGGGAACTAAGTGATTAGGATGTTTTCCTTGCTTGGCTTAGTGGCGAAAACACCATATCTTCTGGCCTGTTATGATTTTAGTTGGTTCAAGAATCACTCTATTGGGGTACCAGGATACCATTTTCTTTAGGGGACTTATTTTCCTAAAATTTTTATATTAGAACTTTATTTGTTAACAAAGCTGTAGATATTCCCTCGACTCCTTGTTTCTCTCTTGCGTGGTCTTCCTCTTCAAGCATACATGGACCCTCAAGTTTTTGGTGTGCTTTGTTTTAAAGGTTTTTTCTCACTTGTTGACTCCATCTTCTTCATCGTTCTTTTCTCTTTGCCAAGTTCTGGAAGGTCACAATCTTGAAGAAATTTTGGCACTTACCTTTTTTGTTGGTTGATTGCACATGGAACTTCAACACAGATTGCTATGTTCTTTATAATCAAGCTAGAATACATTTAAATTTTATGGAGATAACACTTGATGACAAAGATATATGATTGGTTTTAGGAAATTTGTAATCTTACTGTGACTCTTAGGAGGGGATGCTACTTTATGATGGTGAAGGCAGTCATTTTACTTTATGATTGAGAAGTCACTCTTTCACTACTTCATTCGCTGAGGTTGAAGAAAAGTTATTTAGTAGATTGCTTTTTGATGATCGCATTTGGAGAAGCAGATTCAAGTTCCCTATTTTAAGTACACCCTTGTAGTCTATGGCCTAGTGTTTTTTTTGTGGTTTATTCTGGTATTCTTTATTCACAAGCATTAAAGCTTTTTTTCTTTTCTTCCTTTGAATTTATGACTTCTTTTTGGGGGTCCGTTTCCTGTTTGGTTGGCCTGAGCATATTTTCGTTCATTGAACGGTATGTGGCTTTCCATTAAAAGAAAGGGAAATAAAGATAGATATTTCCTGTTACTGAGAGAACACAAATACATTGAAACATTCCTATGCTATCATTTCTGAACTGTTTTATATTTTCTTTATTTAAAACAATTTCATTGATGGTTTTTTTTTGGGGTGTGATTCTCTGCAAGGACAAATGATTGTAGCCCTTTTACTGTAATGGTCACTTATTGCAATTACTTTTTAGTGGCTTTTGCTTCTTGTTTTGGCTTATGTGTTTGTCCTTATTTCCTATAACCTTCAAGCGGTACTTTGGGGGAAAGAGAAACTCTTGCGTCCTAGGAATAACATGTGCAAGTATACATCATTATTAAGAATAAATAAAGAGTATGAAGGAGTTTAAATGGATGCCTCATTAAGTGGCTGCATGGGAACTTTTTTTCCTGGACAAGTTGATCTGCATTATGTCCTAAAGTTTGGCATTATCAACAGGTTCTTCAGGAGAAAGGCATTCATATATGGGATGGCAACGCATCCAGAGATTACCTCGATAGGCAATTGCTTATACATTTAAATTTTGACTTTTTAAATAAGAAACTAGTGAAACTTGAGTTTTCTAATATATAGTTTAAGCTATAATTCTGTTTTCTTTTCCCTTCCTTTTTTCCTTTTTGGAAATCGTAGTATCGGTTTAAGAGAAAGGGAGGAAGGTGACTTGGGACCTGTATATGGGTTCCAGTGGCGACACTTTGGTGCTAGGTTCGTGGATTTTTCTGATGATTGATCCCTTTGAAGTTTGTTTTTATTTTATTGCAGGACTTAAGCTTTAATGCTTTCCACATTTCTTCTACAGGTATACGGACATGCATGCTGACTATACTGGTCAAGGATTTGATCAGTTGCTAGATGTTATTAACAAGATAAAAAATAATCCTGATGACCGGAGGATCATTCTTTCTGCATGGAATCCATCTGATCTCAAACTGATGGCACTTCCTCCTTGCCACATGTTCTCTCAGGTTGGACTCAAATGCATGGTGTCCTATGTTCCCACAACTCATTTCCCTAACATTGTCTATTGATCGCTTTTTGCAGTTTTACGTTGCAAATGGGGAGTTATCATGTCAAATGTATCAGCGTTCTGCTGACATGGGCCTTGGTGTTCCATTTAATATTGCGTCATATGCTCTTCTGACTTGCATGATTGCTCATGTTTGTGGTATGCTACTCCATATTAACTATTAGTTTGATTTCTACTATAGCGATGGCCAAGCATTTAAATTATAATTACCTAGTTGCATTGCTGTCGAACTTTTTTCCCTTTTAAATTTTGGTACAAAGAAGACAGTTTGGTTGGCCAGTATGCGAGGTTTCTTTTTAATGTTATGGGCTGAGTGCTATAAACGTGCCCTTTAAATCTGCGATAGCTTCCTGGACTTGGTTTTGTCTACAGCTTTATGTTCGTACAAAACTAGCACTCTTTTAAGAATTTTAATTTTTCTTATTTAGTTTCCTGTTGAAAATCTGTTTTGCAATCTTTTGGGTCTTCCCTTAATGTTTCCTGAACATACAAAAAAAAAAAAAAAAACTATGTTTCTAAATTATTTCGGAGTTGACCAGGATTTTCAACGATGCAATTATTTGTATATACACCTATTTGTGGCAATCTAGTAAAGTTGTGAACTGAGATTTTTTCTCATCAATATAAGGTTCCTTAAACATTATGAACAATAGTAATGTGGTTTTCGCTCCCCTACAGCTATACTTTTAGTTACTCTAAACAACCAATCCTTTCTATGTGCAGGTCTTGCTCGAGGTGACTTCATACATGTGATTGGAGATGCTCATGTTTACCGTACTCATGTGAGGCCATTGCAGGAACAACTTCAGAAACTTCCCAAACCCTTCCCTGTAAGTAGGAGAACTATAATTACGTGGTTCTTCCATGCTAGCGCTTACTAACCTCCCATTTTCGATATTACATCTTACCTTATTGTAGATTTTGAAGATAAATCCCGAGAAGAAGGATATTGATTCTTTTGTGGCAGATGATTTTGAACTAACTGGTTATGACCCTCATGTGAAGATAGCAATGAAAATGGCAGTATAGAACTTGATCTAACTGTCCAGCAAACAAAGGAAACTCAGTTCCTTTTATCACAATCTCCAAATTTTGAGGTAAGTACATTAAGATTACCATGAAAAATGTTCTTTGAATTGTTTTAAATTGATCATATCAGGTTGAGTAGTCAGTGAAGAACATGTATTCCATGGTACTAAAGCGGTTTACCGATATTTCAGGTACAAGCATCAGAGCCTAATTTGGTTGATAGGGTCTGCAAATAAAAAGTGCTAATTGGATATACTTTATACTTTATCATGTGTAGTGAGAATCCTTTAGTCCCTATGGTTGGGTCTAAAGTGATTTAGTTCGGTTTTGTATTTGCTATAGCTTTCTCTCATTGGAAAATGGAATGGTTTCACACACTTGAATGCACGGTCATGACTATTACAATACCGTGCTACATACAAATATAATGTAAGCTCGACCGATCATTCTCGAACTTCATCTGGTTTTGAAAGGATGGTGAATTTGATAGACTGTGTTGTGACTTGTTGCTTCCTCATTTCTAGTTCTCGCTACCTTAAAAAAATTTGTCCATTATCTCATGTTAGAGAAACAATGGAGTTGGGAGATCTT

mRNA sequence

CAGTTCTCGAATATTCCAATTTGGATCATAGACGGCGATAATCCGCAGCAAGAAACGCGTCTTTGGATTTTCCTCTGTTTTCTTCTTCAAATCTTCTTTCCCACATTTACCCTCTTCCATTACAGACCAAAAAAAGTTAAAAAACCACAAAAAGATCGAAACCAGATAAACAAAAGAAGCGGTTTTTCCATCTAAAAAACTCCCTACCAGCTGGATTTCCCACTCTTCTTTCCTTTCCAGAAATGCCCATTTCGTAAAAACCCTTCTAAATCCATAAACGTTCACCCCCATTTCCTCTCCTAAACCTAACCCTCTTCCAGTCGAAGGTCAGCCTAAGTTTATCTTAGGCTTTTTCGAGTTGAATATTTTCATCCCCCGTTTAGCCTCGGTTCTACCACACTTCCGTGGAAGTTGGGTGTGTGGGGGAAAATCATTGAAGTCACCACTATCTAGGTTTGGTCAAGCTTTTAGTTCTAATATGGCCGCTGATCCTTTGACAATTTCTAATGGCATTGCCAATGTCAATTCACTACCTGATCCACGGAGGACTTATCAAGTTGTTGTAGCTGCTACTGAAAATTTGGGTATCGGTAAGGATGGGAAATTACCATGGAGGTTGCCTTCTGATCTCAAGTTTTTTAAAAACATAACCACAACAACATCAGATCCTGGGAAAAGGAATGCAATTCTAATGGGTCGTAAAACATGGGAGAGTCTTCCTGCCGAGCATCGACCACTACCTGGTCGCCTGAATGTTGTTCTGACTCGTTCTGGAAGTTTTGACATTGCAACTGTTGAAAATGTTATAATATGTGGTAGTATAATGTCTGCTTTGGAATTATTAGCAGCTTCTCCTTACTGTTTGTCAATTGAGAAGGTGTTTGTTATAGGTGGTGGTGAAATATTAAGGGAAGCTATGAATGCCCCTGAATGTGATGCTGTCCACATTACTGAAATTGAGACAAATATTGAATGTGACACCTTCATACCAGCTATTGATAAGTCGATGTTTCAACCATGGTACTCATCTTTTCCTGTGGTTGAAAATAACTTGCGATATTCGTTTAATAGTTATGTTCGTGTGAGGAGCTCAGCTGAACTTCCCATTGATCAGAACAATGGTTTGGTCTCTCATAACAAGCCTGACTTTTCTAACATTGAAGCTAAGAAGTTTTCTTTCCTACCTAAGGAGATTTTCGAGAGGCATGAGGAGCACTTGTACCTAAAACTTGTTCAAGAAATCATCTCAGATGGTATTCCTAAGGATGATAGAACAGGAACAGGTACTTTGTCAAAATTTGGTTGCCAGATGCGGTACAACTTGCGCAAGACTTTTCCACTGCTTACAACTAAGGCATAAAGTATTCTGGCGAGGGGTGGTTGAAGAACTTCTTTGGTTCATTAGTGGTTCAACCAATGCAAAGGTTCTTCAGGAGAAAGGCATTCATATATGGGATGGCAACGCATCCAGAGATTACCTCGATAGTATCGGTTTAAGAGAAAGGGAGGAAGGTGACTTGGGACCTGTATATGGGTTCCAGTGGCGACACTTTGGTGCTAGGTATACGGACATGCATGCTGACTATACTGGTCAAGGATTTGATCAGTTGCTAGATGTTATTAACAAGATAAAAAATAATCCTGATGACCGGAGGATCATTCTTTCTGCATGGAATCCATCTGATCTCAAACTGATGGCACTTCCTCCTTGCCACATGTTCTCTCAGTTTTACGTTGCAAATGGGGAGTTATCATGTCAAATGTATCAGCGTTCTGCTGACATGGGCCTTGGTGTTCCATTTAATATTGCGTCATATGCTCTTCTGACTTGCATGATTGCTCATGTTTGTGGTCTTGCTCGAGGTGACTTCATACATGTGATTGGAGATGCTCATGTTTACCGTACTCATGTGAGGCCATTGCAGGAACAACTTCAGAAACTTCCCAAACCCTTCCCTATTTTGAAGATAAATCCCGAGAAGAAGGATATTGATTCTTTTGTGGCAGATGATTTTGAACTAACTGGTTATGACCCTCATGTGAAGATAGCAATGAAAATGGCAGTATAGAACTTGATCTAACTGTCCAGCAAACAAAGGAAACTCAGTTCCTTTTATCACAATCTCCAAATTTTGAGGTACAAGCATCAGAGCCTAATTTGGTTGATAGGGTCTGCAAATAAAAAGTGCTAATTGGATATACTTTATACTTTATCATGTGTAGTGAGAATCCTTTAGTCCCTATGGTTGGGTCTAAAGTGATTTAGTTCGGTTTTGTATTTGCTATAGCTTTCTCTCATTGGAAAATGGAATGGTTTCACACACTTGAATGCACGGTCATGACTATTACAATACCGTGCTACATACAAATATAATGTAAGCTCGACCGATCATTCTCGAACTTCATCTGGTTTTGAAAGGATGGTGAATTTGATAGACTGTGTTGTGACTTGTTGCTTCCTCATTTCTAGTTCTCGCTACCTTAAAAAAATTTGTCCATTATCTCATGTTAGAGAAACAATGGAGTTGGGAGATCTT

Coding sequence (CDS)

ATGGCCGCTGATCCTTTGACAATTTCTAATGGCATTGCCAATGTCAATTCACTACCTGATCCACGGAGGACTTATCAAGTTGTTGTAGCTGCTACTGAAAATTTGGGTATCGGTAAGGATGGGAAATTACCATGGAGGTTGCCTTCTGATCTCAAGTTTTTTAAAAACATAACCACAACAACATCAGATCCTGGGAAAAGGAATGCAATTCTAATGGGTCGTAAAACATGGGAGAGTCTTCCTGCCGAGCATCGACCACTACCTGGTCGCCTGAATGTTGTTCTGACTCGTTCTGGAAGTTTTGACATTGCAACTGTTGAAAATGTTATAATATGTGGTAGTATAATGTCTGCTTTGGAATTATTAGCAGCTTCTCCTTACTGTTTGTCAATTGAGAAGGTGTTTGTTATAGGTGGTGGTGAAATATTAAGGGAAGCTATGAATGCCCCTGAATGTGATGCTGTCCACATTACTGAAATTGAGACAAATATTGAATGTGACACCTTCATACCAGCTATTGATAAGTCGATGTTTCAACCATGGTACTCATCTTTTCCTGTGGTTGAAAATAACTTGCGATATTCGTTTAATAGTTATGTTCGTGTGAGGAGCTCAGCTGAACTTCCCATTGATCAGAACAATGGTTTGGTCTCTCATAACAAGCCTGACTTTTCTAACATTGAAGCTAAGAAGTTTTCTTTCCTACCTAAGGAGATTTTCGAGAGGCATGAGGAGCACTTGTACCTAAAACTTGTTCAAGAAATCATCTCAGATGGTATTCCTAAGGATGATAGAACAGGAACAGGTACTTTGTCAAAATTTGGTTGCCAGATGCGGTACAACTTGCGCAAGACTTTTCCACTGCTTACAACTAAGGCA

Protein sequence

MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTTTSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALELLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQPWYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIFERHEEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKA
Homology
BLAST of MELO3C023314.jh1 vs. NCBI nr
Match: XP_008460959.1 (PREDICTED: bifunctional dihydrofolate reductase-thymidylate synthase [Cucumis melo] >XP_008460960.1 PREDICTED: bifunctional dihydrofolate reductase-thymidylate synthase [Cucumis melo] >XP_008460961.1 PREDICTED: bifunctional dihydrofolate reductase-thymidylate synthase [Cucumis melo])

HSP 1 Score: 1050 bits (2716), Expect = 0.0
Identity = 514/528 (97.35%), Postives = 518/528 (98.11%), Query Frame = 0

Query: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT
Sbjct: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120
           TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE
Sbjct: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDK+MFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKAMFQP 180

Query: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF
Sbjct: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240

Query: 241 ERHEEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-YPYR-----LILQKVFWRGVV 300
           ERHEE+LYLKLVQEIISDGIPKDDRTGTGTLSKFGCQ+ Y  R     L  +KVFWRGVV
Sbjct: 241 ERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGVV 300

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR
Sbjct: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360

Query: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN 420
           YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN
Sbjct: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN 420

Query: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 480
           GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL
Sbjct: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 480

Query: 481 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 522
           QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV
Sbjct: 481 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 528

BLAST of MELO3C023314.jh1 vs. NCBI nr
Match: TYK28823.1 (bifunctional dihydrofolate reductase-thymidylate synthase [Cucumis melo var. makuwa])

HSP 1 Score: 1050 bits (2716), Expect = 0.0
Identity = 514/528 (97.35%), Postives = 518/528 (98.11%), Query Frame = 0

Query: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT
Sbjct: 49  MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 108

Query: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120
           TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE
Sbjct: 109 TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 168

Query: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDK+MFQP
Sbjct: 169 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKAMFQP 228

Query: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF
Sbjct: 229 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 288

Query: 241 ERHEEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-YPYR-----LILQKVFWRGVV 300
           ERHEE+LYLKLVQEIISDGIPKDDRTGTGTLSKFGCQ+ Y  R     L  +KVFWRGVV
Sbjct: 289 ERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGVV 348

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR
Sbjct: 349 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 408

Query: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN 420
           YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN
Sbjct: 409 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN 468

Query: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 480
           GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL
Sbjct: 469 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 528

Query: 481 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 522
           QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV
Sbjct: 529 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 576

BLAST of MELO3C023314.jh1 vs. NCBI nr
Match: KAA0045571.1 (bifunctional dihydrofolate reductase-thymidylate synthase [Cucumis melo var. makuwa])

HSP 1 Score: 1034 bits (2673), Expect = 0.0
Identity = 511/547 (93.42%), Postives = 516/547 (94.33%), Query Frame = 0

Query: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT
Sbjct: 49  MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 108

Query: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120
           TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE
Sbjct: 109 TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 168

Query: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDK+MFQP
Sbjct: 169 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKAMFQP 228

Query: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF
Sbjct: 229 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 288

Query: 241 ERHEEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-YPYR-----LILQKVFWRGVV 300
           ERHEE+LYLKLVQEIISDGIPKDDRTGTGTLSKFGCQ+ Y  R     L  + +FWRGVV
Sbjct: 289 ERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKALFWRGVV 348

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLD-------------------SIGLRER 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLD                    IGLRER
Sbjct: 349 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHLNFDFLNKKLVKLDIGLRER 408

Query: 361 EEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSD 420
           EEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSD
Sbjct: 409 EEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSD 468

Query: 421 LKLMALPPCHMFSQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARG 480
           LKLMALPPCHMFSQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARG
Sbjct: 469 LKLMALPPCHMFSQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARG 528

Query: 481 DFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVK 522
           DFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVK
Sbjct: 529 DFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVK 588

BLAST of MELO3C023314.jh1 vs. NCBI nr
Match: XP_004148833.1 (bifunctional dihydrofolate reductase-thymidylate synthase [Cucumis sativus] >XP_011649237.1 bifunctional dihydrofolate reductase-thymidylate synthase [Cucumis sativus] >KGN61816.1 hypothetical protein Csa_006327 [Cucumis sativus])

HSP 1 Score: 1021 bits (2640), Expect = 0.0
Identity = 500/528 (94.70%), Postives = 509/528 (96.40%), Query Frame = 0

Query: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLT SNGIANVNS PDPRRTYQVVVAATENLGIGK GKLPWRLPSD+KFFKNIT T
Sbjct: 1   MAADPLTSSNGIANVNSPPDPRRTYQVVVAATENLGIGKGGKLPWRLPSDIKFFKNITIT 60

Query: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120
           TSDPGKRNA+LMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSI SALE
Sbjct: 61  TSDPGKRNAVLMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETN+ECDTFIPA+DKS+FQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNVECDTFIPAVDKSIFQP 180

Query: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240
           WYSSFPVVE NLRYSFNSYVRVRSSAE PIDQNNGLVSHNKPDFSN EAK FSFLPKEIF
Sbjct: 181 WYSSFPVVEKNLRYSFNSYVRVRSSAEPPIDQNNGLVSHNKPDFSNFEAK-FSFLPKEIF 240

Query: 241 ERHEEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-YPYR-----LILQKVFWRGVV 300
           ERHEE+LYLKLVQEIISDGIPKDDRTGTGTLSKFGCQ+ Y  R     L  +KVFWRGVV
Sbjct: 241 ERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGVV 300

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR
Sbjct: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360

Query: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN 420
           YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDL+LMALPPCHMFSQFYVAN
Sbjct: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLQLMALPPCHMFSQFYVAN 420

Query: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 480
           GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL
Sbjct: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 480

Query: 481 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 522
           QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV
Sbjct: 481 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 527

BLAST of MELO3C023314.jh1 vs. NCBI nr
Match: XP_038901596.1 (bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hispida] >XP_038901597.1 bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hispida])

HSP 1 Score: 1012 bits (2616), Expect = 0.0
Identity = 490/528 (92.80%), Postives = 506/528 (95.83%), Query Frame = 0

Query: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60
           MA DPLT SNGIANVN+ PDPRRTYQVVVAA+EN+GIGKDGKLPWRLPSDLKFFKNITTT
Sbjct: 1   MAGDPLTSSNGIANVNAPPDPRRTYQVVVAASENMGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120
           TSD GKRNAILMGRKTWES+PAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSI SALE
Sbjct: 61  TSDHGKRNAILMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFV+GGGEILREA+NAPECDA+HITE+ETNIECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVVGGGEILREALNAPECDAIHITEVETNIECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSF SYVRVRSS E PIDQNNGLVSHNKPDFSN EAKKFSFLPKEIF
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDFSNFEAKKFSFLPKEIF 240

Query: 241 ERHEEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-YPYR-----LILQKVFWRGVV 300
           E+HEE+LYL LVQEIIS+GIPKDDRTGTGTLS+FGCQ+ Y  R     L  +KVFWRGVV
Sbjct: 241 EKHEEYLYLNLVQEIISNGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTKKVFWRGVV 300

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360
           EELLWFISGSTNA VLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR
Sbjct: 301 EELLWFISGSTNANVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360

Query: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN 420
           YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMF+QFYVAN
Sbjct: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVAN 420

Query: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 480
           GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL
Sbjct: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 480

Query: 481 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 522
           QEQLQKLPKPFPILKINPEKKDIDSFV+DDFELTGYDPH KIAMKMAV
Sbjct: 481 QEQLQKLPKPFPILKINPEKKDIDSFVSDDFELTGYDPHEKIAMKMAV 528

BLAST of MELO3C023314.jh1 vs. ExPASy Swiss-Prot
Match: P51820 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 1.3e-242
Identity = 406/531 (76.46%), Postives = 455/531 (85.69%), Query Frame = 0

Query: 1   MAADPLTISNGIAN--VNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNIT 60
           M +D   ISNG +N  VN LP+ +RTYQVVVAAT++ GIGKDGKLPWRLP+DLKFFK IT
Sbjct: 1   MPSDSSVISNGHSNGSVNPLPNLQRTYQVVVAATQDWGIGKDGKLPWRLPTDLKFFKEIT 60

Query: 61  TTTSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSA 120
             TS+PGK+NAI+MGRKTWES+P E+RPL GRLNVVLTRSGSFDIAT ENV+ICGS+ SA
Sbjct: 61  MKTSEPGKKNAIVMGRKTWESIPLEYRPLSGRLNVVLTRSGSFDIATAENVVICGSMSSA 120

Query: 121 LELLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMF 180
           LELLAASPY LSIEKVFVIGGG+I REA+N P C+A+H+TEI+++IECDTF+P +D ++F
Sbjct: 121 LELLAASPYSLSIEKVFVIGGGQIFREALNVPGCEAIHLTEIQSSIECDTFMPPVDFTIF 180

Query: 181 QPWYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKE 240
           +PWYSSFP VENN+RYSF +YVRVRSSA     QN   +  N  +    E K FSFLPK 
Sbjct: 181 RPWYSSFPKVENNIRYSFTTYVRVRSSAAESAGQNIDPLLDNNSESMKFEVKDFSFLPKM 240

Query: 241 IFERHEEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-------YPYRLILQKVFWR 300
           I ERHEE+LYLKLVQ+II++G  K DRTGTGTLSKFGCQ+       +P  L  +KVFWR
Sbjct: 241 ISERHEEYLYLKLVQDIIAEGTTKGDRTGTGTLSKFGCQMRFNLRGNFPL-LTTKKVFWR 300

Query: 301 GVVEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHF 360
           GVVEELLWFISGSTNAKVLQEKGIHIWDGNASR+YLD +GL EREEGDLGPVYGFQWRHF
Sbjct: 301 GVVEELLWFISGSTNAKVLQEKGIHIWDGNASREYLDGVGLTEREEGDLGPVYGFQWRHF 360

Query: 361 GARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFY 420
           GARYTDMH DY+GQGFDQLLDVINKIK NPDDRRIILSAWNP DLKLMALPPCHMF+QFY
Sbjct: 361 GARYTDMHHDYSGQGFDQLLDVINKIKRNPDDRRIILSAWNPVDLKLMALPPCHMFAQFY 420

Query: 421 VANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHV 480
           VA+GELSCQMYQRSADMGLG+PFNIASYALLTCMIAHVC L  GDFIHVIGDAH+YR HV
Sbjct: 421 VAHGELSCQMYQRSADMGLGIPFNIASYALLTCMIAHVCDLIPGDFIHVIGDAHIYRNHV 480

Query: 481 RPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 523
           RPLQEQL   PKPFP LKINP+KKDIDSFVA DF+L GYDPH KI MK++V
Sbjct: 481 RPLQEQLHNQPKPFPTLKINPKKKDIDSFVAADFKLIGYDPHQKIDMKLSV 530

BLAST of MELO3C023314.jh1 vs. ExPASy Swiss-Prot
Match: Q05762 (Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thaliana OX=3702 GN=THY-1 PE=1 SV=2)

HSP 1 Score: 819.3 bits (2115), Expect = 2.5e-236
Identity = 387/523 (74.00%), Postives = 446/523 (85.28%), Query Frame = 0

Query: 7   TISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTTTSDPGK 66
           T  N         +P+RTYQVVVAAT+ +GIGKDGKLPW LP+DLKFFK+IT TTSD  K
Sbjct: 4   TTLNDSVTTTLASEPQRTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDITLTTSDSSK 63

Query: 67  RNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALELLAASP 126
           +NA++MGRKTWES+P ++RPL GRLNVVLTRSG FDIA  ENV+ C S+ SAL+LLAA P
Sbjct: 64  KNAVVMGRKTWESIPIKYRPLSGRLNVVLTRSGGFDIANTENVVTCSSVDSALDLLAAPP 123

Query: 127 YCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQPWYSSFP 186
           YCLSIE+VFVIGGG+ILREA+N P CDA+H+TEI+T+++CDTFIPAID S++QPW SSFP
Sbjct: 124 YCLSIERVFVIGGGDILREALNRPSCDAIHLTEIDTSVDCDTFIPAIDTSVYQPWSSSFP 183

Query: 187 VVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIFERHEEH 246
           V EN LR+ F ++VRV+SSA+   D++NG  S         + KKF FLPK +F++HEE 
Sbjct: 184 VTENGLRFCFTTFVRVKSSADESSDESNGSQS------LQFDGKKFLFLPKMVFDQHEEF 243

Query: 247 LYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-------YPYRLILQKVFWRGVVEELLW 306
           LYL +V++IIS+G  K+DRTGTGTLSKFGCQ+       +P  L  ++VFWRGVVEELLW
Sbjct: 244 LYLNMVEDIISNGNVKNDRTGTGTLSKFGCQMKFNLRRSFPL-LTTKRVFWRGVVEELLW 303

Query: 307 FISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGARYTDMH 366
           FISGSTNAKVLQEKGIHIWDGNASR+YLD IGL EREEGDLGPVYGFQWRHFGA+YTDMH
Sbjct: 304 FISGSTNAKVLQEKGIHIWDGNASREYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMH 363

Query: 367 ADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVANGELSC 426
           ADYTGQGFDQL+DVI+KIKNNPDDRRII+SAWNPSDLKLMALPPCHMF+QFYVA GELSC
Sbjct: 364 ADYTGQGFDQLVDVIDKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVAEGELSC 423

Query: 427 QMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQ 486
           QMYQRSADMGLGVPFNIASY+LLTCM+AHVC L  GDFIHV+GDAHVY+THVRPLQEQL 
Sbjct: 424 QMYQRSADMGLGVPFNIASYSLLTCMLAHVCDLVPGDFIHVLGDAHVYKTHVRPLQEQLL 483

Query: 487 KLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 523
            LPKPFP++KINPEKK IDSFVA DF+LTGYDPH KI MKMAV
Sbjct: 484 NLPKPFPVMKINPEKKQIDSFVASDFDLTGYDPHKKIEMKMAV 519

BLAST of MELO3C023314.jh1 vs. ExPASy Swiss-Prot
Match: P45350 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 3.2e-236
Identity = 387/512 (75.59%), Postives = 444/512 (86.72%), Query Frame = 0

Query: 19  PDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTTTSDPGKRNAILMGRKTWE 78
           PDP+RTYQVVVAAT+N+GIGKDGKLPWRLPSD+KFFK++T TTSDP KRNA++MGRKTWE
Sbjct: 18  PDPQRTYQVVVAATQNMGIGKDGKLPWRLPSDMKFFKDVTMTTSDPLKRNAVIMGRKTWE 77

Query: 79  SLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALELLAASPYCLSIEKVFVIG 138
           S+P +HRPLPGRLNVVLTRSGSFDIATVENV+ICGS++SALELLA SPYC+S+EKVFVIG
Sbjct: 78  SIPIQHRPLPGRLNVVLTRSGSFDIATVENVVICGSMISALELLAGSPYCVSVEKVFVIG 137

Query: 139 GGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQPWYSSFPVVENNLRYSFNS 198
           GG+I REA+NAP CDAVHITEIE +IECDTFIP +D+S+FQPWYSSFP+VEN +RY F +
Sbjct: 138 GGQIYREALNAPGCDAVHITEIEEHIECDTFIPLLDESVFQPWYSSFPLVENKIRYCFTT 197

Query: 199 YVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIFERHEEHLYLKLVQEIISD 258
           YVRVR+S      Q NG  S +K D  N E + FSFLPK +FE+HEE+LYL LV+ IIS+
Sbjct: 198 YVRVRNSVAELTSQTNGCSSDSKSDSGNFEIQNFSFLPKTVFEKHEEYLYLGLVENIISN 257

Query: 259 GIPKDDRTGTGTLSKFGCQI-------YPYRLILQKVFWRGVVEELLWFISGSTNAKVLQ 318
           G+ K+DRT TGT+S FGCQ+       +P  L  +KVFWRGVVEELLWFISGSTNAK+L+
Sbjct: 258 GVTKNDRTRTGTVSIFGCQMRFNLRKSFPL-LTTKKVFWRGVVEELLWFISGSTNAKILK 317

Query: 319 EKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLL 378
           EKG++IW+GN SR+YLDSIGL +REEGDLGP+YGFQWRHFGARYTDMHADY+GQGFDQLL
Sbjct: 318 EKGVNIWEGNGSREYLDSIGLTDREEGDLGPIYGFQWRHFGARYTDMHADYSGQGFDQLL 377

Query: 379 DVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVANGELSCQMYQRSADMGLG 438
           DVI+KIKNNPDDRRII SAWNPSDL+LMALPPCHMF+QFYVANGELSCQMYQRSADMGLG
Sbjct: 378 DVISKIKNNPDDRRIIQSAWNPSDLRLMALPPCHMFAQFYVANGELSCQMYQRSADMGLG 437

Query: 439 VPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQE-QLQKLPKPFPILKI 498
           VPFNIA+YALLTCMIAHVC L  GDF+H IGDAHVY  H+  L E   + LPK FP+LKI
Sbjct: 438 VPFNIAAYALLTCMIAHVCDLVPGDFVHSIGDAHVYSNHLSDLFETSFRMLPKTFPVLKI 497

Query: 499 NPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 523
           N  +KDIDSF A DF+L GYDPH KI MKMAV
Sbjct: 498 NSGEKDIDSFEAADFKLIGYDPHQKIEMKMAV 528

BLAST of MELO3C023314.jh1 vs. ExPASy Swiss-Prot
Match: Q05763 (Bifunctional dihydrofolate reductase-thymidylate synthase 2 OS=Arabidopsis thaliana OX=3702 GN=THY-2 PE=2 SV=2)

HSP 1 Score: 797.3 bits (2058), Expect = 1.0e-229
Identity = 389/526 (73.95%), Postives = 445/526 (84.60%), Query Frame = 0

Query: 7   TISNGI-ANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTTTSDPG 66
           T++N +  NV     P+ TYQVVVAAT+ +GIGKDGKLPW LP+DLKFFK++T +TSD  
Sbjct: 47  TMANTLNGNVIMTSKPQSTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDLTLSTSDSA 106

Query: 67  KRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALELLAAS 126
           K+NA++MGRKTWES+P ++RPL GRLNVVL+RS  FDIA  ENV+ C SI SAL+LLAA 
Sbjct: 107 KKNAVVMGRKTWESIPKKYRPLSGRLNVVLSRSSGFDIANTENVVTCSSIDSALDLLAAP 166

Query: 127 PYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQPWYSSF 186
           P+ LSIEKVFVIGGG+ILREA+N P C+A+HITEI+T+I+CDTFIP +D S +QPW SSF
Sbjct: 167 PFSLSIEKVFVIGGGDILREALNKPSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSSF 226

Query: 187 PVVENNLRYSFNSYVRVR-SSAELPIDQNNGLVSHNKPDFSNIEAKKF-SFLPKEIFERH 246
           P+ EN LR+SF ++VRV+ SSA    D+++G           ++ KKF S LPK IF+RH
Sbjct: 227 PICENGLRFSFTTHVRVKSSSAGEASDESDG------SKVLQVDWKKFSSVLPKMIFDRH 286

Query: 247 EEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-------YPYRLILQKVFWRGVVEE 306
           EE+LYL LV+EIIS+G  KDDRTGTGTLSKFGCQ+       +P  L  ++VFWRGVVEE
Sbjct: 287 EEYLYLNLVKEIISNGNLKDDRTGTGTLSKFGCQMKFNLRRNFPL-LTTKRVFWRGVVEE 346

Query: 307 LLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGARYT 366
           LLWFISGSTNAKVLQEKGI IWDGNASR YLD IGL EREEGDLGPVYGFQWRHFGA+YT
Sbjct: 347 LLWFISGSTNAKVLQEKGIRIWDGNASRAYLDGIGLTEREEGDLGPVYGFQWRHFGAKYT 406

Query: 367 DMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVANGE 426
           DMHADYTGQGFDQLLDVINKIKNNPDDRRII+SAWNPSDLKLMALPPCHMF+QFYVANGE
Sbjct: 407 DMHADYTGQGFDQLLDVINKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVANGE 466

Query: 427 LSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQE 486
           LSCQMYQRSADMGLGVPFNIASY+LLTC++AHVC L  GDFIHVIGDAHVY+ HVRPLQE
Sbjct: 467 LSCQMYQRSADMGLGVPFNIASYSLLTCILAHVCDLVPGDFIHVIGDAHVYKNHVRPLQE 526

Query: 487 QLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 523
           QL+  PKPFP+LKINPEKKDIDSFVADDFEL GYDPH KI MKMAV
Sbjct: 527 QLENPPKPFPVLKINPEKKDIDSFVADDFELIGYDPHKKIDMKMAV 565

BLAST of MELO3C023314.jh1 vs. ExPASy Swiss-Prot
Match: O81395 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Zea mays OX=4577 GN=DRTS PE=2 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 2.2e-229
Identity = 373/518 (72.01%), Postives = 439/518 (84.75%), Query Frame = 0

Query: 12  IANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTTTSDPGKRNAIL 71
           +AN +S   P+R YQVVVA T ++GIGKDG LPW+LP DLKFFK +T TTSDP K+NA++
Sbjct: 5   LANGDSQGRPQRNYQVVVAGTRDMGIGKDGVLPWKLPGDLKFFKELTLTTSDPVKKNAVI 64

Query: 72  MGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALELLAASPYCLSI 131
           MGRKTWES+P + RPLPGRLNV+LTRSGSFD ATVENV+ICGS+ SALELLA++PYCLSI
Sbjct: 65  MGRKTWESIPVKSRPLPGRLNVILTRSGSFDFATVENVVICGSMESALELLASTPYCLSI 124

Query: 132 EKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQPWYSSFPVVENN 191
           EKVFVIGGG++LRE +  P C+A+H+T+I+++IECDTFIP +D S+FQPWYSSFPV+E+N
Sbjct: 125 EKVFVIGGGQVLREYLKGPACEAIHLTDIQSSIECDTFIPPVDFSVFQPWYSSFPVIESN 184

Query: 192 LRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIFERHEEHLYLKL 251
           +R+SF S+VRVR S     + N    +         E + FSFLPK +++RHEE+ YL L
Sbjct: 185 IRHSFVSFVRVRKSVAETHESNGKESTEVDTKNDKFETENFSFLPKMVYDRHEEYQYLNL 244

Query: 252 VQEIISDGIPKDDRTGTGTLSKFGCQI-------YPYRLILQKVFWRGVVEELLWFISGS 311
           V++II  G  K+DRTGTGTLSKFGCQ+       +P  L  ++VFWRGVVEELLWFISGS
Sbjct: 245 VEDIIRSGAQKNDRTGTGTLSKFGCQMRFNLRKNFPL-LTTKRVFWRGVVEELLWFISGS 304

Query: 312 TNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGARYTDMHADYTG 371
           TNAKVLQEKGIHIWDGNASR+YL+S+GL  REEGDLGP+YGFQWRHFGA YTDMHADYTG
Sbjct: 305 TNAKVLQEKGIHIWDGNASREYLNSVGLAHREEGDLGPIYGFQWRHFGAEYTDMHADYTG 364

Query: 372 QGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVANGELSCQMYQR 431
           +GFDQL+DVI+KIKN+P+DRRIILSAWNPSDLK MALPPCHMF+QFYV NGELSCQMYQR
Sbjct: 365 KGFDQLMDVIDKIKNDPEDRRIILSAWNPSDLKKMALPPCHMFAQFYVENGELSCQMYQR 424

Query: 432 SADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKP 491
           SADMGLGVPFNIASY+LLT MIA VC L+ GDF+HVIGDAHVYR HVR L+EQ+QK+PKP
Sbjct: 425 SADMGLGVPFNIASYSLLTYMIAQVCDLSPGDFVHVIGDAHVYRNHVRALEEQIQKMPKP 484

Query: 492 FPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 523
           FPILKINP KKDIDSF+A DF+L GYDPH KI MKMAV
Sbjct: 485 FPILKINPSKKDIDSFMASDFKLVGYDPHQKIEMKMAV 521

BLAST of MELO3C023314.jh1 vs. ExPASy TrEMBL
Match: A0A1S3CE37 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo OX=3656 GN=LOC103499691 PE=3 SV=1)

HSP 1 Score: 1050 bits (2716), Expect = 0.0
Identity = 514/528 (97.35%), Postives = 518/528 (98.11%), Query Frame = 0

Query: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT
Sbjct: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120
           TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE
Sbjct: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDK+MFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKAMFQP 180

Query: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF
Sbjct: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240

Query: 241 ERHEEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-YPYR-----LILQKVFWRGVV 300
           ERHEE+LYLKLVQEIISDGIPKDDRTGTGTLSKFGCQ+ Y  R     L  +KVFWRGVV
Sbjct: 241 ERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGVV 300

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR
Sbjct: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360

Query: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN 420
           YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN
Sbjct: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN 420

Query: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 480
           GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL
Sbjct: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 480

Query: 481 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 522
           QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV
Sbjct: 481 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 528

BLAST of MELO3C023314.jh1 vs. ExPASy TrEMBL
Match: A0A5D3DYK8 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00260 PE=3 SV=1)

HSP 1 Score: 1050 bits (2716), Expect = 0.0
Identity = 514/528 (97.35%), Postives = 518/528 (98.11%), Query Frame = 0

Query: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT
Sbjct: 49  MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 108

Query: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120
           TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE
Sbjct: 109 TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 168

Query: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDK+MFQP
Sbjct: 169 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKAMFQP 228

Query: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF
Sbjct: 229 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 288

Query: 241 ERHEEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-YPYR-----LILQKVFWRGVV 300
           ERHEE+LYLKLVQEIISDGIPKDDRTGTGTLSKFGCQ+ Y  R     L  +KVFWRGVV
Sbjct: 289 ERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGVV 348

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR
Sbjct: 349 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 408

Query: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN 420
           YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN
Sbjct: 409 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN 468

Query: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 480
           GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL
Sbjct: 469 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 528

Query: 481 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 522
           QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV
Sbjct: 529 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 576

BLAST of MELO3C023314.jh1 vs. ExPASy TrEMBL
Match: A0A5A7TVM8 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G00740 PE=3 SV=1)

HSP 1 Score: 1034 bits (2673), Expect = 0.0
Identity = 511/547 (93.42%), Postives = 516/547 (94.33%), Query Frame = 0

Query: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT
Sbjct: 49  MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 108

Query: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120
           TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE
Sbjct: 109 TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 168

Query: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDK+MFQP
Sbjct: 169 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKAMFQP 228

Query: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF
Sbjct: 229 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 288

Query: 241 ERHEEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-YPYR-----LILQKVFWRGVV 300
           ERHEE+LYLKLVQEIISDGIPKDDRTGTGTLSKFGCQ+ Y  R     L  + +FWRGVV
Sbjct: 289 ERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKALFWRGVV 348

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLD-------------------SIGLRER 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLD                    IGLRER
Sbjct: 349 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDRQLLIHLNFDFLNKKLVKLDIGLRER 408

Query: 361 EEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSD 420
           EEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSD
Sbjct: 409 EEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSD 468

Query: 421 LKLMALPPCHMFSQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARG 480
           LKLMALPPCHMFSQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARG
Sbjct: 469 LKLMALPPCHMFSQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARG 528

Query: 481 DFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVK 522
           DFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVK
Sbjct: 529 DFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVK 588

BLAST of MELO3C023314.jh1 vs. ExPASy TrEMBL
Match: A0A0A0LPA5 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis sativus OX=3659 GN=Csa_2G248730 PE=3 SV=1)

HSP 1 Score: 1021 bits (2640), Expect = 0.0
Identity = 500/528 (94.70%), Postives = 509/528 (96.40%), Query Frame = 0

Query: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLT SNGIANVNS PDPRRTYQVVVAATENLGIGK GKLPWRLPSD+KFFKNIT T
Sbjct: 1   MAADPLTSSNGIANVNSPPDPRRTYQVVVAATENLGIGKGGKLPWRLPSDIKFFKNITIT 60

Query: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120
           TSDPGKRNA+LMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSI SALE
Sbjct: 61  TSDPGKRNAVLMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETN+ECDTFIPA+DKS+FQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNVECDTFIPAVDKSIFQP 180

Query: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240
           WYSSFPVVE NLRYSFNSYVRVRSSAE PIDQNNGLVSHNKPDFSN EAK FSFLPKEIF
Sbjct: 181 WYSSFPVVEKNLRYSFNSYVRVRSSAEPPIDQNNGLVSHNKPDFSNFEAK-FSFLPKEIF 240

Query: 241 ERHEEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-YPYR-----LILQKVFWRGVV 300
           ERHEE+LYLKLVQEIISDGIPKDDRTGTGTLSKFGCQ+ Y  R     L  +KVFWRGVV
Sbjct: 241 ERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKKVFWRGVV 300

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR
Sbjct: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360

Query: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN 420
           YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDL+LMALPPCHMFSQFYVAN
Sbjct: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLQLMALPPCHMFSQFYVAN 420

Query: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 480
           GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL
Sbjct: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 480

Query: 481 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 522
           QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV
Sbjct: 481 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 527

BLAST of MELO3C023314.jh1 vs. ExPASy TrEMBL
Match: A0A6J1FWP8 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111448895 PE=3 SV=1)

HSP 1 Score: 993 bits (2567), Expect = 0.0
Identity = 484/528 (91.67%), Postives = 499/528 (94.51%), Query Frame = 0

Query: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60
           MAADPLT SNG ANVN+ P+PRRTYQVVVAATEN+GIGKDGKLPWRLPSDLKFFKNIT T
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60

Query: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120
           TSD GKRNAILMGRKTWES+PAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+ SALE
Sbjct: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREA+NAPECDAVH+TEIETN+ECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240
           WYSSFPVVENNLRYSF SYVRVRSS E PIDQNNGLVSHNKPD  N EAK FSFLPKEIF
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240

Query: 241 ERHEEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-YPYR-----LILQKVFWRGVV 300
           ERHEE+LYLKLVQEIISDG  KDDRTGTGTLSKFGCQ+ Y  R     L  +KVFWRGVV
Sbjct: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTKKVFWRGVV 300

Query: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360
           EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR
Sbjct: 301 EELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGAR 360

Query: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVAN 420
           YTDMHADYTGQGFDQLLDVINKIKNNPDDRRI+LSAWNPSDLKLMALPPCHMF+QFYVAN
Sbjct: 361 YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVAN 420

Query: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPL 480
           GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVC LA GDFIHVIGDAHVYRTHVRPL
Sbjct: 421 GELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRPL 480

Query: 481 QEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 522
           QEQLQKLPKPFP+LKINPEKKDIDSFVA DFELTGYDPH KIAMKMAV
Sbjct: 481 QEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 528

BLAST of MELO3C023314.jh1 vs. TAIR 10
Match: AT2G16370.1 (thymidylate synthase 1 )

HSP 1 Score: 819.3 bits (2115), Expect = 1.8e-237
Identity = 387/523 (74.00%), Postives = 446/523 (85.28%), Query Frame = 0

Query: 7   TISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTTTSDPGK 66
           T  N         +P+RTYQVVVAAT+ +GIGKDGKLPW LP+DLKFFK+IT TTSD  K
Sbjct: 4   TTLNDSVTTTLASEPQRTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDITLTTSDSSK 63

Query: 67  RNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALELLAASP 126
           +NA++MGRKTWES+P ++RPL GRLNVVLTRSG FDIA  ENV+ C S+ SAL+LLAA P
Sbjct: 64  KNAVVMGRKTWESIPIKYRPLSGRLNVVLTRSGGFDIANTENVVTCSSVDSALDLLAAPP 123

Query: 127 YCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQPWYSSFP 186
           YCLSIE+VFVIGGG+ILREA+N P CDA+H+TEI+T+++CDTFIPAID S++QPW SSFP
Sbjct: 124 YCLSIERVFVIGGGDILREALNRPSCDAIHLTEIDTSVDCDTFIPAIDTSVYQPWSSSFP 183

Query: 187 VVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIFERHEEH 246
           V EN LR+ F ++VRV+SSA+   D++NG  S         + KKF FLPK +F++HEE 
Sbjct: 184 VTENGLRFCFTTFVRVKSSADESSDESNGSQS------LQFDGKKFLFLPKMVFDQHEEF 243

Query: 247 LYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-------YPYRLILQKVFWRGVVEELLW 306
           LYL +V++IIS+G  K+DRTGTGTLSKFGCQ+       +P  L  ++VFWRGVVEELLW
Sbjct: 244 LYLNMVEDIISNGNVKNDRTGTGTLSKFGCQMKFNLRRSFPL-LTTKRVFWRGVVEELLW 303

Query: 307 FISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGARYTDMH 366
           FISGSTNAKVLQEKGIHIWDGNASR+YLD IGL EREEGDLGPVYGFQWRHFGA+YTDMH
Sbjct: 304 FISGSTNAKVLQEKGIHIWDGNASREYLDGIGLTEREEGDLGPVYGFQWRHFGAKYTDMH 363

Query: 367 ADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVANGELSC 426
           ADYTGQGFDQL+DVI+KIKNNPDDRRII+SAWNPSDLKLMALPPCHMF+QFYVA GELSC
Sbjct: 364 ADYTGQGFDQLVDVIDKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVAEGELSC 423

Query: 427 QMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQ 486
           QMYQRSADMGLGVPFNIASY+LLTCM+AHVC L  GDFIHV+GDAHVY+THVRPLQEQL 
Sbjct: 424 QMYQRSADMGLGVPFNIASYSLLTCMLAHVCDLVPGDFIHVLGDAHVYKTHVRPLQEQLL 483

Query: 487 KLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 523
            LPKPFP++KINPEKK IDSFVA DF+LTGYDPH KI MKMAV
Sbjct: 484 NLPKPFPVMKINPEKKQIDSFVASDFDLTGYDPHKKIEMKMAV 519

BLAST of MELO3C023314.jh1 vs. TAIR 10
Match: AT4G34570.1 (thymidylate synthase 2 )

HSP 1 Score: 797.3 bits (2058), Expect = 7.1e-231
Identity = 389/526 (73.95%), Postives = 445/526 (84.60%), Query Frame = 0

Query: 7   TISNGI-ANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTTTSDPG 66
           T++N +  NV     P+ TYQVVVAAT+ +GIGKDGKLPW LP+DLKFFK++T +TSD  
Sbjct: 47  TMANTLNGNVIMTSKPQSTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDLTLSTSDSA 106

Query: 67  KRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALELLAAS 126
           K+NA++MGRKTWES+P ++RPL GRLNVVL+RS  FDIA  ENV+ C SI SAL+LLAA 
Sbjct: 107 KKNAVVMGRKTWESIPKKYRPLSGRLNVVLSRSSGFDIANTENVVTCSSIDSALDLLAAP 166

Query: 127 PYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQPWYSSF 186
           P+ LSIEKVFVIGGG+ILREA+N P C+A+HITEI+T+I+CDTFIP +D S +QPW SSF
Sbjct: 167 PFSLSIEKVFVIGGGDILREALNKPSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSSF 226

Query: 187 PVVENNLRYSFNSYVRVR-SSAELPIDQNNGLVSHNKPDFSNIEAKKF-SFLPKEIFERH 246
           P+ EN LR+SF ++VRV+ SSA    D+++G           ++ KKF S LPK IF+RH
Sbjct: 227 PICENGLRFSFTTHVRVKSSSAGEASDESDG------SKVLQVDWKKFSSVLPKMIFDRH 286

Query: 247 EEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-------YPYRLILQKVFWRGVVEE 306
           EE+LYL LV+EIIS+G  KDDRTGTGTLSKFGCQ+       +P  L  ++VFWRGVVEE
Sbjct: 287 EEYLYLNLVKEIISNGNLKDDRTGTGTLSKFGCQMKFNLRRNFPL-LTTKRVFWRGVVEE 346

Query: 307 LLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREEGDLGPVYGFQWRHFGARYT 366
           LLWFISGSTNAKVLQEKGI IWDGNASR YLD IGL EREEGDLGPVYGFQWRHFGA+YT
Sbjct: 347 LLWFISGSTNAKVLQEKGIRIWDGNASRAYLDGIGLTEREEGDLGPVYGFQWRHFGAKYT 406

Query: 367 DMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVANGE 426
           DMHADYTGQGFDQLLDVINKIKNNPDDRRII+SAWNPSDLKLMALPPCHMF+QFYVANGE
Sbjct: 407 DMHADYTGQGFDQLLDVINKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVANGE 466

Query: 427 LSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQE 486
           LSCQMYQRSADMGLGVPFNIASY+LLTC++AHVC L  GDFIHVIGDAHVY+ HVRPLQE
Sbjct: 467 LSCQMYQRSADMGLGVPFNIASYSLLTCILAHVCDLVPGDFIHVIGDAHVYKNHVRPLQE 526

Query: 487 QLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 523
           QL+  PKPFP+LKINPEKKDIDSFVADDFEL GYDPH KI MKMAV
Sbjct: 527 QLENPPKPFPVLKINPEKKDIDSFVADDFELIGYDPHKKIDMKMAV 565

BLAST of MELO3C023314.jh1 vs. TAIR 10
Match: AT2G21550.1 (Bifunctional dihydrofolate reductase/thymidylate synthase )

HSP 1 Score: 554.3 bits (1427), Expect = 1.1e-157
Identity = 285/515 (55.34%), Postives = 363/515 (70.49%), Query Frame = 0

Query: 1   MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 60
           MA+   TI N   +  S+   RR+YQVV+AAT ++G+G D KLPW LPS+ +FF+++TT 
Sbjct: 1   MASTGETICNNGQSAVSV-TRRRSYQVVIAATRDMGLGMDMKLPWDLPSEYQFFQDVTTR 60

Query: 61  TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 120
           TSDP KRNA +MGRK+WES P E RPLPGRLN+VLT+S   +IA  ENV++  S+ SALE
Sbjct: 61  TSDPTKRNATIMGRKSWESTPLEIRPLPGRLNIVLTKSSCHNIAIDENVLVSSSMESALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKSMFQP 180
           LLA  PY LSIEKVFVIGGGE+LR  MNA  CDA+H+TEI+ ++ CD F P +D S+++P
Sbjct: 121 LLATEPYSLSIEKVFVIGGGELLRNYMNASICDAIHLTEIDISVPCDAFAPRVDTSLYRP 180

Query: 181 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 240
           WYSSFPVVEN +RYSFN+YVR + +          + S  K   +  + K++SFLPK +F
Sbjct: 181 WYSSFPVVENGIRYSFNTYVRRKDAI---------VGSGEKKSVAESDLKEYSFLPKMVF 240

Query: 241 ERHEEHLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQI-------YPYRLILQKVFWRGV 300
           ERHEE  YL LVQ IIS G    D     TLSKFGCQ+       +P  L  +K+FW GV
Sbjct: 241 ERHEEFGYLNLVQNIISSG----DMNDNSTLSKFGCQMRFNLRKTFPL-LTTKKIFWLGV 300

Query: 301 VEELLWFISGSTNAKVLQEKGIHIWDGNASRDYLDSIGLREREE-GDLGPVYGFQWRHFG 360
           VEE+L  ISGS N K   E G HIWD + +++YLDS G+   EE GD   ++G  W+H  
Sbjct: 301 VEEILQLISGSNNPK---ENGSHIWDTDEAKEYLDSFGVNATEEDGDNPFLHGLHWKHCD 360

Query: 361 ARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYV 420
           AR+         Q F QL DVINKIKNNP D+RI+L+A NP D KL ++ PCH F+QFYV
Sbjct: 361 ARFVI-------QEFSQLSDVINKIKNNPHDQRIMLAACNPLDFKL-SVSPCHTFTQFYV 420

Query: 421 ANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVR 480
           ANGE+SCQ+YQ S +  +G+PF+IA+Y+LLTC+IAHVC L  GDFIHVIG A++ + HV+
Sbjct: 421 ANGEVSCQIYQSSTEASIGIPFSIATYSLLTCIIAHVCDLGAGDFIHVIGQAYINKAHVK 480

Query: 481 PLQEQLQKLPKPFPILKINPEKKDIDSFVADDFEL 508
            +Q+QLQ  PKPFPILKINPEKK +D+F A D EL
Sbjct: 481 AIQKQLQISPKPFPILKINPEKKKMDNFEASDLEL 489

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008460959.10.097.35PREDICTED: bifunctional dihydrofolate reductase-thymidylate synthase [Cucumis me... [more]
TYK28823.10.097.35bifunctional dihydrofolate reductase-thymidylate synthase [Cucumis melo var. mak... [more]
KAA0045571.10.093.42bifunctional dihydrofolate reductase-thymidylate synthase [Cucumis melo var. mak... [more]
XP_004148833.10.094.70bifunctional dihydrofolate reductase-thymidylate synthase [Cucumis sativus] >XP_... [more]
XP_038901596.10.092.80bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hisp... [more]
Match NameE-valueIdentityDescription
P518201.3e-24276.46Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine max OX=3847... [more]
Q057622.5e-23674.00Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thali... [more]
P453503.2e-23675.59Bifunctional dihydrofolate reductase-thymidylate synthase OS=Daucus carota OX=40... [more]
Q057631.0e-22973.95Bifunctional dihydrofolate reductase-thymidylate synthase 2 OS=Arabidopsis thali... [more]
O813952.2e-22972.01Bifunctional dihydrofolate reductase-thymidylate synthase OS=Zea mays OX=4577 GN... [more]
Match NameE-valueIdentityDescription
A0A1S3CE370.097.35Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo OX=365... [more]
A0A5D3DYK80.097.35Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo var. m... [more]
A0A5A7TVM80.093.42Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo var. m... [more]
A0A0A0LPA50.094.70Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis sativus OX=... [more]
A0A6J1FWP80.091.67Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita moschata ... [more]
Match NameE-valueIdentityDescription
AT2G16370.11.8e-23774.00thymidylate synthase 1 [more]
AT4G34570.17.1e-23173.95thymidylate synthase 2 [more]
AT2G21550.11.1e-15755.34Bifunctional dihydrofolate reductase/thymidylate synthase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00070DHFRcoord: 50..58
score: 46.75
coord: 70..81
score: 61.9
coord: 133..147
score: 41.65
coord: 36..46
score: 58.68
NoneNo IPR availablePANTHERPTHR11548:SF12BIFUNCTIONAL DIHYDROFOLATE REDUCTASE-THYMIDYLATE SYNTHASEcoord: 1..280
IPR024072Dihydrofolate reductase-like domain superfamilyGENE3D3.40.430.10Dihydrofolate Reductase, subunit Acoord: 7..203
e-value: 1.3E-63
score: 216.5
IPR024072Dihydrofolate reductase-like domain superfamilySUPERFAMILY53597Dihydrofolate reductase-likecoord: 22..201
IPR001796Dihydrofolate reductase domainPFAMPF00186DHFR_1coord: 26..201
e-value: 1.1E-38
score: 132.5
IPR001796Dihydrofolate reductase domainPROSITEPS51330DHFR_2coord: 24..201
score: 52.187798
IPR001796Dihydrofolate reductase domainCDDcd00209DHFRcoord: 28..200
e-value: 2.85038E-63
score: 195.049
IPR036926Thymidylate synthase/dCMP hydroxymethylase superfamilyGENE3D3.30.572.10Thymidylate synthase/dCMP hydroxymethylase domaincoord: 232..296
e-value: 1.1E-7
score: 32.8
IPR036926Thymidylate synthase/dCMP hydroxymethylase superfamilySUPERFAMILY55831Thymidylate synthase/dCMP hydroxymethylasecoord: 226..281
IPR045097Thymidylate synthase/dCMP hydroxymethylasePANTHERPTHR11548THYMIDYLATE SYNTHASE 1coord: 1..280
IPR017925Dihydrofolate reductase conserved sitePROSITEPS00075DHFR_1coord: 36..58

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C023314.jh1.t2MELO3C023314.jh1.t2mRNA
MELO3C023314.jh1.t3MELO3C023314.jh1.t3mRNA
MELO3C023314.jh1.t1MELO3C023314.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006231 dTMP biosynthetic process
biological_process GO:0006730 one-carbon metabolic process
biological_process GO:0046654 tetrahydrofolate biosynthetic process
molecular_function GO:0004146 dihydrofolate reductase activity
molecular_function GO:0004799 thymidylate synthase activity
molecular_function GO:0016741 transferase activity, transferring one-carbon groups