MELO3C022247 (gene) Melon (DHL92) v4

Overview


Name	MELO3C022247
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	cytochrome b5
Location	chr11: 29702071 .. 29703773 (-)
RNA-Seq Expression	MELO3C022247
Synteny	MELO3C022247

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAACCAATAAAAATTAAAATACTCAAAAATGAATCAAATTAAAGTGTAGGATTACAATAATTTACATGATATTTCTTTCTAAGGCTAAGAGATTTGTTCTTTCCATCCTATATAAAATATTATACTAAACAACAAAAGCTAAAATAAATACACCAGAAATGCAACATTTTTTCTAAAACAATCATTTAGAAGTAATTGTTGTATTGCTCTCAATATTTTCCGTCTTTTCTTTTCATTTTGATATCTCTTCTACTTCTTCTTCTTCTTCTTATCTTCTTCTATTTAAGGTCTAAAATATTGAAACTCTGTTCAAGGGAAGGATTAGAAAAAAAAAAGTTGATAGGAAATGGATTCAAATTCAAAAGTGTATTCTTTTGATGAAGTTGCAAAGCACAACCACCAGAAGGATTGTTGGCTTATAATCTCTGGATATGTATGTTCTTCAATTTCCCTTTTCTTCTTTTTGAAGTCTTTCTCTAATTACAACACATATTCATTGCTTCTATTACATCATCTTAAAGATTTCGATTATGAGAAACATGCAGAAGTTTCTTCCGTGTGACAATCTTTTTCTCTCTCACGTTTTCATAATGTTCTAAGGCATTCTCTTGTCTCTAAGTTTTAGAAGAGTAGTGTGAGTGTTGTTGTCTTATCAGTACTCCTTGTCTGGAGTGGTTCTGGAGGGTTATTGTGAATTATGATGTTGTTTCTAGATTGTTTGTTAGTTTTGCTGTTTTGTTTCTGTATTACCCTAGCTTTGGTAGATGAATTGTTTTTAGGTGTACGATGTGACTTCATTTTTGCCGGACCATCCAGGTGGCGATGAGCTTCTGCTACTTGCAGTGGGTGAGATGCTACACACCCATTTCTATAATCATACAAATCCATCTCTAATCAATTGCATGCTTTTTTCTAATGTTTTATATATGCACACATTAACCTGGTTTCATTAGTATACATTTTACTGATCTTTATTCTAGTTTATATATACTTTTAGAAATGATCTTAAAATTAGTCTTCTGTTGTTTCTTTGAAATATTAAGATTCATGTAACTTTTCAAAGACATTTTTGCATAAACGACTATGGAATCACGGCCCAATTTTAACATTTTGCTTTAAGTAGTCACATAGCACTATATATACAAAGAGAGGGCTAAAACTTCGATTTTCTTGCTGTCACTAGTTGTAGCAGAAAAATGAATTGAGCTTCTAACTTTGTTGTTAGTATTACATTTTTTTTCCTTGTATTTATTACTTTATCATTTTGATGATGACTAGAGTCGAATTATGTTTGCGGATTAATTAACGTGATAAAATTGAATATATGCAGAAAAAGATGCGACATTTGATTTTAAGAGCGTTGGACATAGCGAATTAGCTCAAGAGAAGATGAAAATGTATCGAATAGGGAAGATTGACATGTCGACTCTCCCTAAAAAACAAAATTACGTGGAAAGTACACCATATCAGCCAACTCCGAGTCTATTTTCCTTCTCACCCATCCATGTTCTACTCCCTATTTTGCTCTTGGCCTTGGCTTTTGCGTTTCCCTACTTCAAACTTAAGGCCTAAAATTCATAAGTTTCCATTTCTATTTTACTTTCCCACAAGGATTATAGCCTATGGGCTATACAATAACAATTTGTAGCAACTTCATTATTGAATGATTCAATGGAATTTTTACGTCTTTATCATGAACTTAA

mRNA sequence

GAACCAATAAAAATTAAAATACTCAAAAATGAATCAAATTAAAGTGTAGGATTACAATAATTTACATGATATTTCTTTCTAAGGCTAAGAGATTTGTTCTTTCCATCCTATATAAAATATTATACTAAACAACAAAAGCTAAAATAAATACACCAGAAATGCAACATTTTTTCTAAAACAATCATTTAGAAGTAATTGTTGTATTGCTCTCAATATTTTCCGTCTTTTCTTTTCATTTTGATATCTCTTCTACTTCTTCTTCTTCTTCTTATCTTCTTCTATTTAAGGTCTAAAATATTGAAACTCTGTTCAAGGGAAGGATTAGAAAAAAAAAAGTTGATAGGAAATGGATTCAAATTCAAAAGTGTATTCTTTTGATGAAGTTGCAAAGCACAACCACCAGAAGGATTGTTGGCTTATAATCTCTGGATATGTGTACGATGTGACTTCATTTTTGCCGGACCATCCAGGTGGCGATGAGCTTCTGCTACTTGCAGTGGAAAAAGATGCGACATTTGATTTTAAGAGCGTTGGACATAGCGAATTAGCTCAAGAGAAGATGAAAATGTATCGAATAGGGAAGATTGACATGTCGACTCTCCCTAAAAAACAAAATTACGTGGAAAGTACACCATATCAGCCAACTCCGAGTCTATTTTCCTTCTCACCCATCCATGTTCTACTCCCTATTTTGCTCTTGGCCTTGGCTTTTGCGTTTCCCTACTTCAAACTTAAGGCCTAAAATTCATAAGTTTCCATTTCTATTTTACTTTCCCACAAGGATTATAGCCTATGGGCTATACAATAACAATTTGTAGCAACTTCATTATTGAATGATTCAATGGAATTTTTACGTCTTTATCATGAACTTAA

Coding sequence (CDS)

ATGGATTCAAATTCAAAAGTGTATTCTTTTGATGAAGTTGCAAAGCACAACCACCAGAAGGATTGTTGGCTTATAATCTCTGGATATGTGTACGATGTGACTTCATTTTTGCCGGACCATCCAGGTGGCGATGAGCTTCTGCTACTTGCAGTGGAAAAAGATGCGACATTTGATTTTAAGAGCGTTGGACATAGCGAATTAGCTCAAGAGAAGATGAAAATGTATCGAATAGGGAAGATTGACATGTCGACTCTCCCTAAAAAACAAAATTACGTGGAAAGTACACCATATCAGCCAACTCCGAGTCTATTTTCCTTCTCACCCATCCATGTTCTACTCCCTATTTTGCTCTTGGCCTTGGCTTTTGCGTTTCCCTACTTCAAACTTAAGGCCTAA

Protein sequence

MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFKSVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLALAFAFPYFKLKA

Homology

[Close]

BLAST of MELO3C022247 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 127.9 bits (320), Expect = 8.6e-29
Identity = 70/136 (51.47%), Postives = 89/136 (65.44%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+ KV SF+EV+KHN  KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQP------TPSLFSFSPIHVLLP 120
            VGHS+ A++ M  Y IG+ID S++P  + YV   P QP      TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPE-FIIKILQFLVP 120

Query: 121 ILLLALAFAFPYFKLK 131
           IL+L LA    ++  K
Sbjct: 121 ILILGLALVVRHYTKK 133

[Close]

BLAST of MELO3C022247 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 3.3e-28
Identity = 69/136 (50.74%), Postives = 87/136 (63.97%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S  KV  F+EV++HN  KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQP------TPSLFSFSPIHVLLP 120
            VGHS+ A++ M+ Y IG+ID ST+P  + YV   P QP      TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYV--APVQPAYNQDKTPE-FMIKILQFLVP 120

Query: 121 ILLLALAFAFPYFKLK 131
           IL+L LA     +  K
Sbjct: 121 ILILGLALVVRQYTKK 133

[Close]

BLAST of MELO3C022247 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 5.2e-26
Identity = 61/132 (46.21%), Postives = 84/132 (63.64%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M   +KV++  EV++HN+ KDCWL+ISG VYDVT FL DHPGGDE+LL A  KDAT DF+
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQP-----TPSLFSFSPIHVLLPI 120
            VGHS  A+  +  Y +G ID +T+P K  Y  + P QP       S F    +  L+P+
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKY--TPPNQPHYNQDKTSEFVVKLLQFLVPL 120

Query: 121 LLLALAFAFPYF 128
           ++L +AF   ++
Sbjct: 121 IILGVAFGIRFY 130

[Close]

BLAST of MELO3C022247 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.2e-25
Identity = 58/126 (46.03%), Postives = 83/126 (65.87%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M   +K+++  EV++HN   DCW++I+G VY+VT FL DHPGGD++LL +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQP-----TPSLFSFSPIHVLLPI 120
            VGHSE A+E M+ Y +G+ID +T+PKK  Y  + P QP       S F    +  L+P+
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY--TPPKQPHYNQDKTSEFIIKLLQFLVPL 120

Query: 121 LLLALA 122
            +L LA
Sbjct: 121 AILGLA 124

[Close]

BLAST of MELO3C022247 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 117.1 bits (292), Expect = 1.5e-25
Identity = 63/128 (49.22%), Postives = 81/128 (63.28%), Query Frame = 0


Query: 2   DSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFKS 61
           + N KVY+ +EVAKHN + DCWLII G VY+V+ FL DHPGGD++LL +  KDAT DF+ 
Sbjct: 3   NDNKKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFED 62

Query: 62  VGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQP------TPSLFSFSPIHVLLPI 121
           VGHS  A+  M  Y +G ID ST+P +  YV   P QP      TP  F    +  L+P+
Sbjct: 63  VGHSTTARAMMDEYYVGDIDTSTIPARTKYV--PPKQPHYNQDKTPE-FIIKILQFLVPL 122

Query: 122 LLLALAFA 124
            +L LA A
Sbjct: 123 AILGLAVA 127

[Close]

BLAST of MELO3C022247 vs. NCBI nr
Match: XP_008459741.1 (PREDICTED: cytochrome b5 [Cucumis melo])

HSP 1 Score: 267.3 bits (682), Expect = 6.9e-68
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120
           SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of MELO3C022247 vs. NCBI nr
Match: XP_004140502.1 (cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cucumis sativus])

HSP 1 Score: 250.4 bits (638), Expect = 8.7e-63
Identity = 123/131 (93.89%), Postives = 126/131 (96.18%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHN QKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120
           SVGHSELA EKMKMY+IGKIDMSTLP+KQ YVES PYQ TPSLFSFSPIH+LLPILLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of MELO3C022247 vs. NCBI nr
Match: XP_038875836.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 244.6 bits (623), Expect = 4.8e-61
Identity = 116/131 (88.55%), Postives = 127/131 (96.95%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDS+SK+YSFDEV+KHNH +DCWLIISGYVYDVT+FLPDHPGG+ELLLLAV +DATFDFK
Sbjct: 3   MDSSSKLYSFDEVSKHNHHRDCWLIISGYVYDVTAFLPDHPGGEELLLLAVGRDATFDFK 62

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120
           SVGHSELAQEKMK YRIGKIDMSTLP+KQ YVE+TPYQPTPSLFSFSPIH+L+PILLLAL
Sbjct: 63  SVGHSELAQEKMKKYRIGKIDMSTLPRKQQYVETTPYQPTPSLFSFSPIHLLIPILLLAL 122

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 123 AFAFPYFKLKA 133

[Close]

BLAST of MELO3C022247 vs. NCBI nr
Match: XP_023000205.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 223.8 bits (569), Expect = 8.8e-55
Identity = 104/131 (79.39%), Postives = 119/131 (90.84%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSK+Y+F+EVAKHNH+KDCWLIISGYVYDVTS+LPDHPGG ELL+L VEKDATFDFK
Sbjct: 1   MDSNSKIYTFNEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGSELLVLTVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120
           SVGHSE A+E MK Y+IGKID  TLP +Q YVE+TPY+PTPSL SFSP+HVL+P+LLL L
Sbjct: 61  SVGHSETAREMMKKYQIGKIDPLTLPTEQKYVETTPYKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of MELO3C022247 vs. NCBI nr
Match: XP_022964486.1 (cytochrome b5-like [Cucurbita moschata])

HSP 1 Score: 222.6 bits (566), Expect = 2.0e-54
Identity = 104/131 (79.39%), Postives = 121/131 (92.37%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVY+FDEVAKHNH+KDCWLIISG+VYDVTS+LPDHPGG ELL+LAVEKDATFDFK
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120
           SVGHSE A+E+MK Y+IGKID  TLP +Q +VE+TP++PTPSL SFSP+HVL+P+LLL L
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of MELO3C022247 vs. ExPASy TrEMBL
Match: A0A1S3CAE5 (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 3.3e-68
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120
           SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of MELO3C022247 vs. ExPASy TrEMBL
Match: A0A0A0KA20 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G107940 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 4.2e-63
Identity = 123/131 (93.89%), Postives = 126/131 (96.18%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVYSFDEVAKHN QKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120
           SVGHSELA EKMKMY+IGKIDMSTLP+KQ YVES PYQ TPSLFSFSPIH+LLPILLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

[Close]

BLAST of MELO3C022247 vs. ExPASy TrEMBL
Match: A0A6J1KHQ2 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 4.2e-55
Identity = 104/131 (79.39%), Postives = 119/131 (90.84%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSK+Y+F+EVAKHNH+KDCWLIISGYVYDVTS+LPDHPGG ELL+L VEKDATFDFK
Sbjct: 1   MDSNSKIYTFNEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGSELLVLTVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120
           SVGHSE A+E MK Y+IGKID  TLP +Q YVE+TPY+PTPSL SFSP+HVL+P+LLL L
Sbjct: 61  SVGHSETAREMMKKYQIGKIDPLTLPTEQKYVETTPYKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of MELO3C022247 vs. ExPASy TrEMBL
Match: A0A6J1HJ10 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 9.4e-55
Identity = 104/131 (79.39%), Postives = 121/131 (92.37%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           MDSNSKVY+FDEVAKHNH+KDCWLIISG+VYDVTS+LPDHPGG ELL+LAVEKDATFDFK
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120
           SVGHSE A+E+MK Y+IGKID  TLP +Q +VE+TP++PTPSL SFSP+HVL+P+LLL L
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 121 AFAFPYFKLKA 132
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

[Close]

BLAST of MELO3C022247 vs. ExPASy TrEMBL
Match: A0A6J5WK33 (Cytochrome b5 heme-binding domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS15953 PE=3 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 2.4e-34
Identity = 77/133 (57.89%), Postives = 98/133 (73.68%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+S+VY FDEVAKHNH+KDCW+IISG VYDVT FL DHPGGDE+LLLAVEKDAT DF 
Sbjct: 1   MASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKDATDDFN 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNY---VESTPYQPTPSLFSFSPIHVLLPILL 120
            VGHS+ A+E+M+ + +GK+D ST+P++ NY    ++TP     S F    +  LLP+L+
Sbjct: 61  DVGHSDSAKEQMEKFYVGKVDTSTIPEQPNYKLPAQTTPPSGQSSGFVVKLLQFLLPLLI 120

Query: 121 LALAFAFPYFKLK 131
           L  AFA  Y+  K
Sbjct: 121 LGAAFALQYYGKK 133

[Close]

BLAST of MELO3C022247 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 127.9 bits (320), Expect = 6.1e-30
Identity = 70/136 (51.47%), Postives = 89/136 (65.44%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M S+ KV SF+EV+KHN  KDCWLIISG VYDVT F+ DHPGGDE+LL +  KDAT DF+
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQP------TPSLFSFSPIHVLLP 120
            VGHS+ A++ M  Y IG+ID S++P  + YV   P QP      TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPE-FIIKILQFLVP 120

Query: 121 ILLLALAFAFPYFKLK 131
           IL+L LA    ++  K
Sbjct: 121 ILILGLALVVRHYTKK 133

[Close]

BLAST of MELO3C022247 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 117.5 bits (293), Expect = 8.2e-27
Identity = 58/126 (46.03%), Postives = 83/126 (65.87%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M   +K+++  EV++HN   DCW++I+G VY+VT FL DHPGGD++LL +  KDAT DF+
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQP-----TPSLFSFSPIHVLLPI 120
            VGHSE A+E M+ Y +G+ID +T+PKK  Y  + P QP       S F    +  L+P+
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY--TPPKQPHYNQDKTSEFIIKLLQFLVPL 120

Query: 121 LLLALA 122
            +L LA
Sbjct: 121 AILGLA 124

[Close]

BLAST of MELO3C022247 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 116.7 bits (291), Expect = 1.4e-26
Identity = 61/137 (44.53%), Postives = 84/137 (61.31%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M  + KV++  EV++H+  KDCW++I G VYDVT FL DHPGGDE++L +  KDAT DF+
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVEST-----PYQPTPSLFSFSPIHVLLPI 120
            VGHS  A+  +  Y +G ID +T+P K  +V  T       Q   S F    +  L+P+
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 LLLALAFAFPYF-KLKA 132
           L+L LAF   Y+ K KA
Sbjct: 121 LILGLAFGIRYYTKTKA 137

[Close]

BLAST of MELO3C022247 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 110.5 bits (275), Expect = 1.0e-24
Identity = 55/131 (41.98%), Postives = 82/131 (62.60%), Query Frame = 0


Query: 5   SKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFKSVGH 64
           + + SF +VAKH  + DCW++I G VYD+++F+ +HPGGD +LL    KDA+ DF+ V H
Sbjct: 2   ANLISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNH 61

Query: 65  SELAQEKMKMYRIGKIDMSTLPKKQNYV-----ESTPYQPTPSLFSFSPIHVLLPILLLA 124
           S+ A+E MK Y IG +D ST+P  Q Y+     EST  + T        +  L+P+L+L 
Sbjct: 62  SKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILG 121

Query: 125 LAFAFPYFKLK 131
           +AFA  ++  K
Sbjct: 122 VAFALRFYNNK 132

[Close]

BLAST of MELO3C022247 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 107.5 bits (267), Expect = 8.5e-24
Identity = 51/126 (40.48%), Postives = 82/126 (65.08%), Query Frame = 0


Query: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60
           M + +K+YS +E A HN Q DCW++I G VYDV+S++ +HPGGD++LL    KDAT DF+
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  SVGHSELAQEKMKMYRIGKIDMSTL---PKKQNYVESTPYQPTPSLFSFSPIHVLLPILL 120
             GHS+ A+E M+ Y IG++D S+L   P+ + Y +  P      LF  +  + ++P+ +
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 LALAFA 124
           + ++ A
Sbjct: 121 ITISVA 126

The following BLAST results are available for this feature:

BLAST of MELO3C022247 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42342	8.6e-29	51.47	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
P40934	3.3e-28	50.74	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
P49098	5.2e-26	46.21	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
O48845	1.2e-25	46.03	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
P49100	1.5e-25	49.22	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]

BLAST of MELO3C022247 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008459741.1	6.9e-68	100.00	PREDICTED: cytochrome b5 [Cucumis melo]	[more]
XP_004140502.1	8.7e-63	93.89	cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cuc...	[more]
XP_038875836.1	4.8e-61	88.55	cytochrome b5-like [Benincasa hispida]	[more]
XP_023000205.1	8.8e-55	79.39	cytochrome b5-like [Cucurbita maxima]	[more]
XP_022964486.1	2.0e-54	79.39	cytochrome b5-like [Cucurbita moschata]	[more]

BLAST of MELO3C022247 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CAE5	3.3e-68	100.00	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1	[more]
A0A0A0KA20	4.2e-63	93.89	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A6J1KHQ2	4.2e-55	79.39	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1	[more]
A0A6J1HJ10	9.4e-55	79.39	cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1	[more]
A0A6J5WK33	2.4e-34	57.89	Cytochrome b5 heme-binding domain-containing protein OS=Prunus armeniaca OX=3659...	[more]

BLAST of MELO3C022247 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53560.1	6.1e-30	51.47	cytochrome B5 isoform E	[more]
AT2G32720.1	8.2e-27	46.03	cytochrome B5 isoform B	[more]
AT5G48810.1	1.4e-26	44.53	cytochrome B5 isoform D	[more]
AT2G46650.1	1.0e-24	41.98	cytochrome B5 isoform C	[more]
AT1G26340.1	8.5e-24	40.48	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 30..40 score: 64.74 coord: 55..62 score: 35.8 coord: 40..54 score: 44.55 coord: 68..80 score: 35.78
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 6.4E-26 score: 102.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 e-value: 4.6E-22 score: 77.9
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 26.711296
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 1..84 e-value: 6.4E-31 score: 108.2
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..89
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..129
None	No IPR available	PANTHER	PTHR19359:SF81	SUBFAMILY NOT NAMED	coord: 1..129
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C022247.1	MELO3C022247.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding