MELO3C022070.jh1 (gene) Melon (Harukei-3) v1.41

BLAST of MELO3C022070.jh1 vs. NCBI nr
Match: XP_008459428.1 (PREDICTED: putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo] >ADN34285.1 beta-glucan-binding protein [Cucumis melo subsp. melo])

HSP 1 Score: 1454 bits (3764), Expect = 0.0
Identity = 727/727 (100.00%), Postives = 727/727 (100.00%), Query Frame = 0

Query: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST 60
           MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST 60

Query: 61  QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI 120
           QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI
Sbjct: 61  QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI 120

Query: 121 LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF 180
           LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF
Sbjct: 121 LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF 180

Query: 181 LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS 240
           LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS
Sbjct: 181 LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS 240

Query: 241 GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA 300
           GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA
Sbjct: 241 GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA 300

Query: 301 HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF 360
           HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF
Sbjct: 301 HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF 360

Query: 361 AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLI 420
           AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLI
Sbjct: 361 AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLI 420

Query: 421 GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL 480
           GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL
Sbjct: 421 GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL 480

Query: 481 VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ 540
           VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ
Sbjct: 481 VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ 540

Query: 541 ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK 600
           ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK
Sbjct: 541 ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK 600

Query: 601 ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS 660
           ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS
Sbjct: 601 ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS 660

Query: 661 LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS 720
           LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS
Sbjct: 661 LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS 720

Query: 721 NSKIHLV 727
           NSKIHLV
Sbjct: 721 NSKIHLV 727

BLAST of MELO3C022070.jh1 vs. NCBI nr
Match: KAA0039393.1 (putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo var. makuwa] >TYK00581.1 putative endo-1,3(4)-beta-glucanase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1453 bits (3761), Expect = 0.0
Identity = 726/727 (99.86%), Postives = 727/727 (100.00%), Query Frame = 0

Query: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST 60
           MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST 60

Query: 61  QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI 120
           QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI
Sbjct: 61  QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI 120

Query: 121 LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF 180
           LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF
Sbjct: 121 LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF 180

Query: 181 LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS 240
           LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS
Sbjct: 181 LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS 240

Query: 241 GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA 300
           GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA
Sbjct: 241 GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA 300

Query: 301 HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF 360
           HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF
Sbjct: 301 HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF 360

Query: 361 AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLI 420
           AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFL+
Sbjct: 361 AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLM 420

Query: 421 GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL 480
           GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL
Sbjct: 421 GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL 480

Query: 481 VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ 540
           VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ
Sbjct: 481 VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ 540

Query: 541 ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK 600
           ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK
Sbjct: 541 ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK 600

Query: 601 ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS 660
           ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS
Sbjct: 601 ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS 660

Query: 661 LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS 720
           LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS
Sbjct: 661 LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS 720

Query: 721 NSKIHLV 727
           NSKIHLV
Sbjct: 721 NSKIHLV 727

BLAST of MELO3C022070.jh1 vs. NCBI nr
Match: XP_011656028.1 (endo-1,3(4)-beta-glucanase 2 [Cucumis sativus] >KGN52559.1 hypothetical protein Csa_008697 [Cucumis sativus])

HSP 1 Score: 1399 bits (3620), Expect = 0.0
Identity = 703/736 (95.52%), Postives = 714/736 (97.01%), Query Frame = 0

Query: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPP---------QTPESQPPEPSQKPSE 60
           M KKIER L R  KGKRHSPP  LPPPPPPPPPPP         Q PESQPPEPSQKPSE
Sbjct: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE 60

Query: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120
           PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS
Sbjct: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120

Query: 121 SISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180
           SISVSYPSILS  ASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR
Sbjct: 121 SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180

Query: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240
           FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL
Sbjct: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240

Query: 241 THNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETK 300
           THNLSMITSGGFAGIIRIAALPNSDLECE+ILDRFSSCYPVLGEAQ TKPFCLEYKWETK
Sbjct: 241 THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK 300

Query: 301 GWGDLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360
           GWGDLLMLAHPLHLRLL GSDDNV+ILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS
Sbjct: 301 GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360

Query: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEV 420
           IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLE+
Sbjct: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM 420

Query: 421 VPEIRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLG 480
           VPEIRKFLIGAIEPWLNGTFEGNGF+YDEKWGGIV+KEGAFD+SADFGFG+YNNHHHHLG
Sbjct: 421 VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG 480

Query: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGL 540
           YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFD YKLHSWGTGL
Sbjct: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL 540

Query: 541 AEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGET 600
           +EFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGS+LAALEIKAGQMWWQIREGET
Sbjct: 541 SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET 600

Query: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660
           TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR
Sbjct: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660

Query: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720
           ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS
Sbjct: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720

Query: 721 RGKEEENISNSKIHLV 727
           RGKEE+NI+NSKIHLV
Sbjct: 721 RGKEEQNINNSKIHLV 736

BLAST of MELO3C022070.jh1 vs. NCBI nr
Match: XP_038889399.1 (endo-1,3(4)-beta-glucanase 2-like [Benincasa hispida])

HSP 1 Score: 1343 bits (3477), Expect = 0.0
Identity = 675/733 (92.09%), Postives = 698/733 (95.23%), Query Frame = 0

Query: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPP-----PPPPPPQTPESQPPEPSQKPSEPTPF 60
           MFKKIER LR   KGKR  PP  LPPPPP     PPPPPPQ PESQPPEPSQKPSEPTPF
Sbjct: 1   MFKKIERGLRWVIKGKRRFPPPHLPPPPPLPPPPPPPPPPQPPESQPPEPSQKPSEPTPF 60

Query: 61  LFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISV 120
           LFPSTQSTVLPDPS+FFSPELLSAPLP+NSFFQNFTLNNGDQPEYIHPYLIKSSLSSISV
Sbjct: 61  LFPSTQSTVLPDPSLFFSPELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISV 120

Query: 121 SYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLV 180
           SYPSI S  ASECQIFTPDLTISPSEK+NPLPQKSHVISSFNDL+VTLDIPSSNLRFYLV
Sbjct: 121 SYPSISSNSASECQIFTPDLTISPSEKMNPLPQKSHVISSFNDLNVTLDIPSSNLRFYLV 180

Query: 181 RGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNL 240
           RGSPFLTFTVSKG+AFSISTIHEV+SFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNL
Sbjct: 181 RGSPFLTFTVSKGIAFSISTIHEVLSFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNL 240

Query: 241 SMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGD 300
           S+ITSGGFAGIIRIAAL NSD ECE ILDRFSSCYPV GEAQFTKPFCLEYKWETKGWGD
Sbjct: 241 SIITSGGFAGIIRIAALSNSDPECELILDRFSSCYPVSGEAQFTKPFCLEYKWETKGWGD 300

Query: 301 LLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGV 360
           LLMLAHPLHLRLL GSD++V+ILDKFKYKSIDGELVGVVGSSWALKPE ISVSWHSIRGV
Sbjct: 301 LLMLAHPLHLRLLAGSDNDVVILDKFKYKSIDGELVGVVGSSWALKPESISVSWHSIRGV 360

Query: 361 EEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEI 420
           EEESFAEIISAL KDV ALNSTS ILTTKSPYSYGKLIARAARLAVIAEEVR LEVVPEI
Sbjct: 361 EEESFAEIISALGKDVGALNSTSKILTTKSPYSYGKLIARAARLAVIAEEVRFLEVVPEI 420

Query: 421 RKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLY 480
           RKFL+GAIEPWLNGTFEGNGF++DEKWGGIV+KEG+FD  ADFGFG+YNNHHHHLGYFLY
Sbjct: 421 RKFLVGAIEPWLNGTFEGNGFVHDEKWGGIVSKEGSFDSGADFGFGMYNNHHHHLGYFLY 480

Query: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFT 540
           AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSR+ANSKFPKLRCFD YKLHSWGTGLAEFT
Sbjct: 481 AIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRQANSKFPKLRCFDAYKLHSWGTGLAEFT 540

Query: 541 DGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYK 600
           DGRSQES+SEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQ+REGETTLY 
Sbjct: 541 DGRSQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGETTLYH 600

Query: 601 EEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVN 660
           EEFVKENRVVGVLWSNKRD GLWFAPSEWKECRLGIQVLPILPITELLLSD GFVRELVN
Sbjct: 601 EEFVKENRVVGVLWSNKRDCGLWFAPSEWKECRLGIQVLPILPITELLLSDAGFVRELVN 660

Query: 661 WALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKE 720
           WALPSLGREGVGEGWKGFV+ALESIYDKDGSLQKIRNLK+FDDGNSLTNLLWWVHSRGKE
Sbjct: 661 WALPSLGREGVGEGWKGFVYALESIYDKDGSLQKIRNLKDFDDGNSLTNLLWWVHSRGKE 720

Query: 721 -EENISNSKIHLV 727
            E+NISNSK+ LV
Sbjct: 721 GEKNISNSKVQLV 733

BLAST of MELO3C022070.jh1 vs. NCBI nr
Match: XP_022134296.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Momordica charantia])

HSP 1 Score: 1281 bits (3314), Expect = 0.0
Identity = 643/736 (87.36%), Postives = 683/736 (92.80%), Query Frame = 0

Query: 1   MFKKIERELRRAFKGKRHS-PPLRLPPPPPPPPPPP-------QTPESQPPEPSQKPSEP 60
           MFKKIERELRRA KGKR S PP RLPPPPPPPPPPP       Q P+S PPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60

Query: 61  TPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120
           TPFLFP TQSTVLPDPS FFS ELLSAPLP+NSFFQNFTLNNGDQPEYIHPYLIKSSLSS
Sbjct: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRF 180
           ISVSYPSI S  ASE QIFTPDLTI PSEKI PLPQKSHVISSFNDL+VTLDIPSSNLRF
Sbjct: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180

Query: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLT 240
           +LVRGSPFLTFTVSKG AFSIS  HEV+SFSFNNALTKYT+KLKNNQTWLIYSS PINLT
Sbjct: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240

Query: 241 HNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKG 300
           HNLS+ITSGGFAGIIRIAALPNSD ECE ILDRFSSCYPV GEA FTKPFC+EYKWET+G
Sbjct: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300

Query: 301 WGDLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSI 360
           WGDLLMLAHPLH+RLL G++++V+ILDKFKYKSIDG+LVGVVGS W LKPEP+SV WHSI
Sbjct: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360

Query: 361 RGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVV 420
           RGVEEESFAEIISALRKDVEALNST+M  TT SPYSYGKLIARAARLAVIAEEVR LEV+
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTTM--TTTSPYSYGKLIARAARLAVIAEEVRCLEVI 420

Query: 421 PEIRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGY 480
           PEIRKFL+GAIEPWLNGTF+GNGF+YD KWGGIV+KEG+FD  ADFGFGIYNNHHHHLGY
Sbjct: 421 PEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGY 480

Query: 481 FLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLA 540
           FLYAIAVLVKIDPAWGRKYSPQ YSLMADIMNLSRR+NSKFP+LRCFD YKLHSWGTGL 
Sbjct: 481 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLT 540

Query: 541 EFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETT 600
           EF DGR QES+SEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQ+REGET 
Sbjct: 541 EFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGET- 600

Query: 601 LYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRE 660
           LY+EEFVKENR+VGVLWSNKRDSGLWFAP EW+EC+LGIQVLP+LPITELL SDVGFVRE
Sbjct: 601 LYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRE 660

Query: 661 LVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSR 720
           LVNW LPSLGREGVGEGWKGFV+ALESIYDKDG+L+KIRNLK+FDDGNSLTNLLWWVHSR
Sbjct: 661 LVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSR 720

Query: 721 GKE-EENISNSKIHLV 727
           GKE E NIS++K+ LV
Sbjct: 721 GKEGEANISSNKVQLV 733

BLAST of MELO3C022070.jh1 vs. ExPASy Swiss-Prot
Match: Q09850 (Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 9.0e-35
Identity = 164/672 (24.40%), Postives = 284/672 (42.26%), Query Frame = 0

Query: 58  PSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSY 117
           P   S   P P +  +  L   P+ +N F+ N  +     P + HPY +  +  + S  Y
Sbjct: 14  PVFPSRAHPSPPLGLTSNLF--PIQTNKFYGNLYIGTRHNPSWSHPYSV--TWLNGSSYY 73

Query: 118 PSILSTCASECQIFTPDLTISPSE-KINPLPQKSHVISSFNDLSVTLDIPSSNLRFYL-- 177
              +S      ++F PD    P +   NP    S +IS+    S  L     +  F +  
Sbjct: 74  GLAISHIDDSQRVFGPDPESVPCQYYFNPAGLYSIIISAREFASGNLLSLDQSRHFSIQA 133

Query: 178 -----VRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALT-------------KYTIKLK 237
                  GS  +   +  G+ F +S  +  ++  FN+++              KY I+L 
Sbjct: 134 TLSATTSGSGTIILPIVAGMGF-VSGYYTNLTPVFNSSILFSSITKINFSRGYKYRIQLT 193

Query: 238 NNQTWLIY-------SSFPINLTHNLSMITSGGFAGIIRIAALPNSDLE---CERILDRF 297
           + + W +Y       S+F + L  N  + TS  F G+I+I  +PN  +     + I D  
Sbjct: 194 DGKIWFLYAFPTSSSSTFNLTLASNSQLTTSTKFTGLIQICKVPNESVNNSYPDTIYDAS 253

Query: 298 SSCYPV-LGEAQFTKPFCLEY--KWETKGWGDL--LMLAHPLHLRLLRGSDDNVIILDKF 357
           +  Y   +  +        EY  ++ T G+ +L  LM A P H++   GSD         
Sbjct: 254 AGVYTTSISLSAQVSGTTGEYWFRFATAGYTNLNPLMFALPHHMQSF-GSDTQAYKTGLG 313

Query: 358 KYKSIDGELVGVVGSSWAL--KPEPISVSWHSI------RGVEEESFAEIISALRKDV-- 417
              +  G +      +W L  K  P  V +  I            + A I +A   D+  
Sbjct: 314 LASTTMGIMFAYATKTWHLIEKNLPTQVGFLPIPWNGGSNTYSPTALAAIRAACATDINF 373

Query: 418 EALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLIGAIEPWLNGTF 477
           + +N++++     S Y+ GK++A  A++ ++A  +     +       +           
Sbjct: 374 DVVNASNL----DSMYTSGKIVAMYAQVCLVASRILGDSTLTNTGLTKLKQAMARFTTNT 433

Query: 478 EGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVLVKIDPAW-GRK 537
           +    +YD  + GI++  G     AD+G   YN+HH H GY +YA AV+  +DP+W    
Sbjct: 434 QMYPLVYDTTYKGIISTAGYSSPLADYGNTYYNDHHFHWGYHIYACAVIGLLDPSWLVND 493

Query: 538 YSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQESVSEAVNAY 597
               V +L+ D  N S  +++ F   R FD +  HSW TG+ E  DG+ +ES SE  N  
Sbjct: 494 NIRYVNALLRDSANPS-ESDTYFAMFRNFDWFVGHSWATGIFESGDGKDEESTSEDFNFL 553

Query: 598 YSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVKENRVVGVLWS 657
           Y+  L G+   D  L++  +++  L +    +   I    TT  +   +  N V G+ + 
Sbjct: 554 YATKLWGMVRNDTVLINRANLM--LAVLKNSLNTYIYMTPTTSVQPSQILGNYVTGITFM 613

Query: 658 NKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPSLGREGVGEGW 683
           NK D   +F+  E+  C+ GI ++P  PI+  L S   +V++  N  +  +        W
Sbjct: 614 NKVDYATYFSAEEY--CKQGIHMIPTTPISGYLRSP-SYVQQDWNAKIAPI-INTFSNNW 668

BLAST of MELO3C022070.jh1 vs. ExPASy Swiss-Prot
Match: Q9UT45 (Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 4.4e-34
Identity = 171/677 (25.26%), Postives = 284/677 (41.95%), Query Frame = 0

Query: 73  SPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLI----KSSLSSISVSYPSILSTCASEC 132
           S + LS+P+ +N FF N  L +     Y  P+        ++  I +++           
Sbjct: 74  STDSLSSPIETNKFFDNNLLGSRTNFMYADPFRYWWQSSDTMGGICIAHTD------DNQ 133

Query: 133 QIFTPDLTISPSEKINPLP------QKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPFLT 192
           ++   D TI PS    P+         S + S+ + +   +D  S+   F     S  LT
Sbjct: 134 RVMDTDDTI-PSYYYEPIGICSLGFGASGITSNTDPIVDEIDQMSARFTFSWDSSSMQLT 193

Query: 193 FTVSKGVAFSIST------------IHEVISFSFNNALTKYTIKLKNNQTWLIY---SSF 252
            T    V  ++ T            I++ +     +A+ KY + + +N  WLIY    S 
Sbjct: 194 LTEGMAVTTAVYTNAIPQIFSSTLYINDFVEVPGTSAVQKYRVTMSDNHVWLIYIYGDSL 253

Query: 253 PINLTHNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPV-LGEAQFTKPFCLEY 312
            +  + +  ++ S  F G I+IA +P  D   E + D ++  Y   +  + + +     Y
Sbjct: 254 TLTESTSQMLVGSNTFNGYIQIAKIPLGDGTAEALYDTYAGVYITGISISGYVEDAVGYY 313

Query: 313 KWETKGWGD------LLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWAL 372
            ++    GD        +L H +    + G+    I+L       + G++    G+S   
Sbjct: 314 SFDFTTAGDTSVEPLFFLLPHQVD-TAVSGTKVTSIVLASL----VSGDMNAAAGNSITF 373

Query: 373 K---PEPIS-VSWHSIRGV--EEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIA 432
               P+ I  + W    G     E   EII+ +    E     S      S Y  GK++A
Sbjct: 374 AEAIPQDIGFLPWSPTGGQIGYSEEALEIIAEV-AGTELGEDFSANSNLNSMYYSGKVLA 433

Query: 433 RAARLAVIAEEVRSLEVVPE--IRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEG-A 492
           + A L V   ++   E   E  I+K L  A   +++   +     YD  W G+V+  G +
Sbjct: 434 KYAMLCVTINDILGDETSSEQCIQK-LEAAFARFVDNQ-QIYPLTYDNTWKGVVSVAGLS 493

Query: 493 FDHSADFGFGIYNNHHHHLGYFLYAIAVLVKIDPAWGRKYSPQ--VYSLMADIMNLSRRA 552
            D  ADFG   YN+HH H GYF++  AV+  IDP W    + +  V  L+ D+ N S   
Sbjct: 494 GDSLADFGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINTGNNKEWVNFLVRDVANPSSN- 553

Query: 553 NSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIG 612
           +  FPK R  D Y  H W +GL E  DG+ +ES SE  N ++   L G   GD+ +    
Sbjct: 554 DPYFPKHRMIDIYHGHGWASGLFESNDGKDEESTSEDYNFFFGMKLWGQVIGDSDMEDRA 613

Query: 613 SMLAALEIKAGQMWWQIREGETTLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRL 672
           +++  +E  A   +    +G     +   ++ N V G+ + NK     +F  +   E   
Sbjct: 614 NIILGIERNALNKYMLYADGNV---QPTSMQPNYVAGITFMNKITHTTYFGTN--IEYIQ 673

Query: 673 GIQVLPILPITELLLSDVGFVRELVNW-ALPSLGREGVGEGWKGFVHALESIYDKDGSLQ 702
           GI +LPI PI+  +     FV  L  W AL +   + V  GW+  ++A  +I + + S +
Sbjct: 674 GIHMLPITPISAFIRGP-SFV--LAEWNALLASVIDYVDSGWRSLLYANLAIAEPEESYE 726

BLAST of MELO3C022070.jh1 vs. ExPASy Swiss-Prot
Match: P53753 (Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 2.3e-30
Identity = 161/682 (23.61%), Postives = 279/682 (40.91%), Query Frame = 0

Query: 58   PSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSY 117
            P T     P+P           P+ +N F+ N  + + + P +++PY +    SS   SY
Sbjct: 411  PPTVFDRSPNPMSLADGVSNDGPIQTNKFYTNLIVGSQESPAFVYPYSLWKYTSS---SY 470

Query: 118  P-SILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFN-DLSVTLDI---------- 177
              ++  T   +      D + +    +NPL     V S+ N D S+T+ +          
Sbjct: 471  GFAVQHTTVDQYSYGGYDSSGNAEYLVNPLGIAHVVFSASNFDSSMTMQVDEMTLSSTRV 530

Query: 178  -------PSSNLRFYLVRGSPFLT------FTVSKGVAFSISTIHEVISFSFNNALTKYT 237
                    S+ L   LV+G  F T           G +   +TI    S +    + KY 
Sbjct: 531  VLSESNDSSNYLEIPLVQGMGFATGIYHGSLNAKIGSSVGFNTIVSESSSNLAQGILKYR 590

Query: 238  IKLKNNQTWLIY---------SSFPINLTHNLSMITSGGFAGIIRIAALPNSDLECERIL 297
            I L N  TWL Y         + F + ++    +  S    G+I   A+  S+ + E   
Sbjct: 591  ITLLNGVTWLCYVIGPDDLTSTDFSLEVSSEYEIKASASVDGLIIQLAVAPSETDYEVFY 650

Query: 298  DRFSSCYPVLGEAQFT---KPFCLEYKWETKG---WGDLLMLAHPLHLRLLRGSDDNVII 357
            D+ +  Y    + Q          E+ + T+G    G  ++ A P H      S  + I+
Sbjct: 651  DQAAGMYVTNFKLQGVSDGSTATYEFSYTTQGESASGSTMIFALPHH-----ESSFSDIM 710

Query: 358  LDKFKYKSIDGELVGVVGS--------SWALKPEPISVSWHSIRGVEEESFAEIISALRK 417
             D +    +     GV+          S +L  +   + W S  G     +++    L  
Sbjct: 711  QDYYTGIQLASTTKGVMNGYLTTSLQFSTSLNRQISWLPWSSQLGSNLLEYSKEQLQLLA 770

Query: 418  DV---EALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLIGAIEPW 477
            +V   E   S S  ++  + Y  GK+I + + + +   E+   E   +     I +    
Sbjct: 771  EVANSELQVSISESISGLNTYYLGKVIDKYSYILLTVSEIIQDEASTKSTLENIKSAFDI 830

Query: 478  LNGTFEGNGFLYDEKWGGIVTKE--GAFDHSADFGFGIYNNHHHHLGYFLYAIAVL---- 537
            L    +    +YD K+ G+V+    G+     DFG   YN+HH H GY ++A AV+    
Sbjct: 831  LLQNEQTYPLIYDTKFNGLVSSGDWGSTSTQYDFGNTYYNDHHFHYGYIIHAAAVIGYVD 890

Query: 538  VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ 597
             K++  W       V SL+ D+ N S + +  F + R FD +  HSW  GL E  +G+++
Sbjct: 891  SKLNGTWAADNKDWVNSLVRDVANPSEK-DEYFAQSRMFDWFNGHSWAAGLYENGNGKNE 950

Query: 598  ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK 657
            ES SE  N  Y+  L G   GD  +   G ++ ++ +K     +   + + T+  EE + 
Sbjct: 951  ESSSEDYNFAYAMKLWGATIGDQSMELRGDLMISI-MKDAMNDYFYYQNDNTVEPEEII- 1010

Query: 658  ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS 683
             N+V G+L+ N  D   +F  +   E   GI +LPI P++  + S+     E      P 
Sbjct: 1011 GNKVSGILFDNIIDYTTYFGTN--TEYIHGIHMLPITPVSSNIRSETFVEEEWQTKIEPI 1070

BLAST of MELO3C022070.jh1 vs. ExPASy Swiss-Prot
Match: Q12168 (Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.6e-28
Identity = 193/792 (24.37%), Postives = 324/792 (40.91%), Query Frame = 0

Query: 24  LPPPPP---------PPPPPPQTPESQPP----EPSQKPSE--------PTPFLFPSTQS 83
           +PPP P          PPP P     QPP    E S KP E         TP        
Sbjct: 8   IPPPVPNRPGGTTNRGPPPLPPRANVQPPVCSSENSSKPRENRVAGESLRTPSSSNPLAD 67

Query: 84  TVLPDPSVFFSPELLSA-------------------------PLPSNSFFQNFTLNNGDQ 143
           + +   ++F SP L +                          PL +N F+ N  L++  Q
Sbjct: 68  SQVNSDNIFQSPVLSNLKAPPSVFNKVQHPVPKPNIDDQSVDPLETNKFYTNMLLDDNTQ 127

Query: 144 PEYIHPYLIKSS----LSSISVSYPSILSTCASECQIFTPDLTISPSE-KINPLPQKSHV 203
           P + HPY I  S    L  ++ ++     T AS+ ++F  D T +P     NP   KS V
Sbjct: 128 PIWTHPYSIWFSRDPELFGLAANH-----TLASQ-RVF--DTTTNPPRFYFNPTNIKSFV 187

Query: 204 IS-----SFNDLSVTL-DIPSSNLRFYL-VRGSPFLTFTVSKGVAFSISTIHEV------ 263
                  S ND+ +   D+   ++   + +  S F+ F + +G+ F  +  H++      
Sbjct: 188 FKAREFVSSNDIKLEFRDMKHMSMCLLMSLSSSQFIEFPLVQGMGFVTAIYHDLGFELRS 247

Query: 264 -ISF------SFNNALTKYTIKLKNNQTWLIY---------SSFPINLTHNLSMITSGGF 323
            + F      S N    KY I+L+NN+ W++Y           F I+L  + ++I+S   
Sbjct: 248 AVGFRSLERISVNERYGKYNIQLENNRNWILYLTSPDYSFPQDFQISLLDSNTIISSHKI 307

Query: 324 AGIIRIAALPNSDLECERI--LDRFSSCYPVL----GEAQFTKPFCLEYKWETKGW---G 383
            G+I         L  + +  +D  + CYPV     G+          + +   G+   G
Sbjct: 308 NGLI-------CQLSADSVPSIDMAAGCYPVYCDLSGQTVDEHFTNYRFNYTVAGYSQSG 367

Query: 384 DLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKP--------EPIS 443
             LM A P H            I       ++ G + G + +S+ ++         EP++
Sbjct: 368 TTLMYALPHHKAAFTPEMQEREIASSLD-STVKGLMTGYLTNSFDMQVQVPQELGFEPVA 427

Query: 444 VSWHSIRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEV 503
           +S +      +E  ++I  A  ++V+ L+         S Y  GK++A+ A +  +   +
Sbjct: 428 LSLNKKADYSQEKLSKIREAAVQEVQ-LSDPQQESNIDSMYFSGKILAKYAWILYVTHYI 487

Query: 504 RSLE-VVPEIRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNN 563
              E +  E+   L  A+E +++   +     YD  W GI++   +   S DFG   YN+
Sbjct: 488 LHDENLTKELLSKLTIAMERFISNQ-QVLPLNYDVSWKGIISSGSS---SQDFGNSYYND 547

Query: 564 HHHHLGYFLYAIAVLVKIDP--------AWGRKYSPQVYSLMADIMNLSRRANSKFPKLR 623
           HH H  Y +   A++  +D         +W       V  L+ D   +    +  FP+ R
Sbjct: 548 HHFHYSYHVITAAIISLVDSDLSGVTNNSWLENNRDWVECLIRDYSGVD-NDDPYFPQFR 607

Query: 624 CFDPYKLHSWGTGLAEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEI 683
            FD +  HSW  GL    DG+ +ES SE VN+ Y+  L GL  G++ L  I ++   +  
Sbjct: 608 SFDWFNGHSWAKGLFPSGDGKDEESTSEDVNSCYAIKLWGLVTGNSKLTDIANLQLGIMR 667

Query: 684 KAGQMWWQIREGETTLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPIL 704
              Q ++ + E   T+  +EF+  N+V G+L+ NK D   +F           I ++  +
Sbjct: 668 NVFQSYF-LYESNNTVQPKEFI-GNKVSGILFENKIDHATYFGMEPQY-----IHMIHAI 727

BLAST of MELO3C022070.jh1 vs. ExPASy Swiss-Prot
Match: D4AZ24 (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 9.9e-26
Identity = 195/776 (25.13%), Postives = 317/776 (40.85%), Query Frame = 0

Query: 16  KRHSPPLRLPPPPPP---------------PPPPPQTPESQ-PPEPSQKPS--------- 75
           KR   P R PP P                 P P   +P S  P +PS  P+         
Sbjct: 136 KRSPAPQRHPPAPTKATAGYQFTNCTSTSNPGPTATSPTSGIPSQPSAPPATMAGQDIFQ 195

Query: 76  ----EPTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLI 135
               +P P    S     +    +    E  + P+ +N F+ NF L N     + HPY +
Sbjct: 196 PIAKDPIPANIKSRDDHPVKANHI----ENPTGPISTNKFYANFFLGNQTSTTFTHPYTM 255

Query: 136 -------KSSLSSISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFN-D 195
                   +S   +++S+         E     P   +     INP+  KS V+S+    
Sbjct: 256 IWAKGDKNASSFGMAISHVEPSQRATGEPNNKLPGNPV--RYYINPVGIKSLVLSASELK 315

Query: 196 LSVTLDI--PSSNLRFYLVR----GSPFLTFTVSKGVAFSISTIHEVISFSFNNA----- 255
            S T+ +  P +     ++R     S  +TF + +G+ F I+ I+  +  +  +A     
Sbjct: 316 ESTTMSVAKPQAFSAQAILRPTGGSSESITFPLVQGMGF-ITAIYNNLQPAIQSAVLFRK 375

Query: 256 ----------LTKYTIKLKNNQTWLIY------SSFPINLTHNLSMITSGGFAGIIRIAA 315
                     + KY I L++++ WL+Y      +   + L  N  +    GF G+I++A 
Sbjct: 376 VEPAGSPQGGIFKYKITLEDDKNWLLYVIPENGADPKLKLEGNKLISGPTGFKGVIQVAK 435

Query: 316 LPNSDLECERILDRFSSCY----PVLGEAQFTKPFCLEYKWETKGWG-DLLMLAHPLHLR 375
            P+++ E E I D+ +  Y     + G          ++ +E  G G  L+M A P H+ 
Sbjct: 436 NPSAE-EGEGIYDKSAGSYATDIKISGSVGTDGTGTYKFSFEKAGKGAPLVMYALPHHVE 495

Query: 376 LLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVS-----W---HSIRGVEEE 435
               +  N     K    +  G     VG SW +    + +S     W    S +    E
Sbjct: 496 SFDDATKNTKKNMKLS-TTTKGMATACVGDSWTMVEGNLPLSMDFAPWKPGSSSQVTLSE 555

Query: 436 SFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIAR-AARLAVIAEEVRSLEVVPEIRK 495
                I A+  + E      +     S Y  GK + + A  +  + E V           
Sbjct: 556 GAKNAIKAVAGN-ELSQDMELQTNLNSMYFSGKGLNKFAGAIYTVQELVGDKAAASGPLN 615

Query: 496 FLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAF---DHSADFGFGIYNNHHHHLGYFL 555
            L  + + +++   +    +YD  W G+V+  G +   D   DFG  +YN+HH H GYF+
Sbjct: 616 SLKESFKRFVDNK-QQIPLVYDNVWKGVVS-SGTYEKGDTGLDFGNTLYNDHHFHYGYFI 675

Query: 556 YAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEF 615
              A+L K+DPAW       V  L+ D  N S   +  FP  R FD Y  HSW  GL E 
Sbjct: 676 LTAAILGKLDPAWLDANKAYVNMLVRDSGN-SVDNDEHFPFSRAFDWYHGHSWAKGLFES 735

Query: 616 TDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLY 675
           +DG+ QES SE     Y+  + G   GD  + + G+++  +  +    ++ + + +    
Sbjct: 736 SDGKDQESTSEDTMYAYAIKMWGKTSGDKSMEARGNLMLGILARTLNNYF-LMKNDNVNQ 795

Query: 676 KEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELV 702
            + F+  N+V G+L+ NK D   +F  +   E   GI +LP+LP +    S   FV+E  
Sbjct: 796 PKNFI-GNKVTGILFENKIDHTTYFGAN--LEYIQGIHMLPLLPNSAYTRS-AEFVKE-- 855

BLAST of MELO3C022070.jh1 vs. ExPASy TrEMBL
Match: E5GCT6 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1454 bits (3764), Expect = 0.0
Identity = 727/727 (100.00%), Postives = 727/727 (100.00%), Query Frame = 0

Query: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST 60
           MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST 60

Query: 61  QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI 120
           QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI
Sbjct: 61  QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI 120

Query: 121 LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF 180
           LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF
Sbjct: 121 LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF 180

Query: 181 LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS 240
           LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS
Sbjct: 181 LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS 240

Query: 241 GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA 300
           GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA
Sbjct: 241 GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA 300

Query: 301 HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF 360
           HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF
Sbjct: 301 HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF 360

Query: 361 AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLI 420
           AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLI
Sbjct: 361 AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLI 420

Query: 421 GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL 480
           GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL
Sbjct: 421 GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL 480

Query: 481 VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ 540
           VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ
Sbjct: 481 VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ 540

Query: 541 ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK 600
           ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK
Sbjct: 541 ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK 600

Query: 601 ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS 660
           ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS
Sbjct: 601 ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS 660

Query: 661 LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS 720
           LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS
Sbjct: 661 LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS 720

Query: 721 NSKIHLV 727
           NSKIHLV
Sbjct: 721 NSKIHLV 727

BLAST of MELO3C022070.jh1 vs. ExPASy TrEMBL
Match: A0A1S3CBD8 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo OX=3656 GN=LOC103498569 PE=3 SV=1)

HSP 1 Score: 1454 bits (3764), Expect = 0.0
Identity = 727/727 (100.00%), Postives = 727/727 (100.00%), Query Frame = 0

Query: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST 60
           MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST 60

Query: 61  QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI 120
           QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI
Sbjct: 61  QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI 120

Query: 121 LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF 180
           LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF
Sbjct: 121 LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF 180

Query: 181 LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS 240
           LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS
Sbjct: 181 LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS 240

Query: 241 GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA 300
           GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA
Sbjct: 241 GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA 300

Query: 301 HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF 360
           HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF
Sbjct: 301 HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF 360

Query: 361 AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLI 420
           AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLI
Sbjct: 361 AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLI 420

Query: 421 GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL 480
           GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL
Sbjct: 421 GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL 480

Query: 481 VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ 540
           VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ
Sbjct: 481 VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ 540

Query: 541 ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK 600
           ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK
Sbjct: 541 ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK 600

Query: 601 ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS 660
           ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS
Sbjct: 601 ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS 660

Query: 661 LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS 720
           LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS
Sbjct: 661 LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS 720

Query: 721 NSKIHLV 727
           NSKIHLV
Sbjct: 721 NSKIHLV 727

BLAST of MELO3C022070.jh1 vs. ExPASy TrEMBL
Match: A0A5A7T8V6 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001970 PE=3 SV=1)

HSP 1 Score: 1453 bits (3761), Expect = 0.0
Identity = 726/727 (99.86%), Postives = 727/727 (100.00%), Query Frame = 0

Query: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST 60
           MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST
Sbjct: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFLFPST 60

Query: 61  QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI 120
           QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI
Sbjct: 61  QSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVSYPSI 120

Query: 121 LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF 180
           LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF
Sbjct: 121 LSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVRGSPF 180

Query: 181 LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS 240
           LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS
Sbjct: 181 LTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHNLSMITS 240

Query: 241 GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA 300
           GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA
Sbjct: 241 GGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWGDLLMLA 300

Query: 301 HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF 360
           HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF
Sbjct: 301 HPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRGVEEESF 360

Query: 361 AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLI 420
           AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFL+
Sbjct: 361 AEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPEIRKFLM 420

Query: 421 GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL 480
           GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL
Sbjct: 421 GAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFLYAIAVL 480

Query: 481 VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ 540
           VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ
Sbjct: 481 VKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQ 540

Query: 541 ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK 600
           ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK
Sbjct: 541 ESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETTLYKEEFVK 600

Query: 601 ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS 660
           ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS
Sbjct: 601 ENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRELVNWALPS 660

Query: 661 LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS 720
           LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS
Sbjct: 661 LGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSRGKEEENIS 720

Query: 721 NSKIHLV 727
           NSKIHLV
Sbjct: 721 NSKIHLV 727

BLAST of MELO3C022070.jh1 vs. ExPASy TrEMBL
Match: A0A0A0KUJ9 (Endo-1,3(4)-beta-glucanase OS=Cucumis sativus OX=3659 GN=Csa_5G643380 PE=3 SV=1)

HSP 1 Score: 1399 bits (3620), Expect = 0.0
Identity = 703/736 (95.52%), Postives = 714/736 (97.01%), Query Frame = 0

Query: 1   MFKKIERELRRAFKGKRHSPPLRLPPPPPPPPPPP---------QTPESQPPEPSQKPSE 60
           M KKIER L R  KGKRHSPP  LPPPPPPPPPPP         Q PESQPPEPSQKPSE
Sbjct: 1   MLKKIERLLGRVIKGKRHSPPWCLPPPPPPPPPPPSPPPSPPPPQPPESQPPEPSQKPSE 60

Query: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120
           PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS
Sbjct: 61  PTPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLS 120

Query: 121 SISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180
           SISVSYPSILS  ASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR
Sbjct: 121 SISVSYPSILSNSASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLR 180

Query: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240
           FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL
Sbjct: 181 FYLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINL 240

Query: 241 THNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETK 300
           THNLSMITSGGFAGIIRIAALPNSDLECE+ILDRFSSCYPVLGEAQ TKPFCLEYKWETK
Sbjct: 241 THNLSMITSGGFAGIIRIAALPNSDLECEQILDRFSSCYPVLGEAQITKPFCLEYKWETK 300

Query: 301 GWGDLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360
           GWGDLLMLAHPLHLRLL GSDDNV+ILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS
Sbjct: 301 GWGDLLMLAHPLHLRLLGGSDDNVVILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHS 360

Query: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEV 420
           IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLE+
Sbjct: 361 IRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEM 420

Query: 421 VPEIRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLG 480
           VPEIRKFLIGAIEPWLNGTFEGNGF+YDEKWGGIV+KEGAFD+SADFGFG+YNNHHHHLG
Sbjct: 421 VPEIRKFLIGAIEPWLNGTFEGNGFVYDEKWGGIVSKEGAFDYSADFGFGMYNNHHHHLG 480

Query: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGL 540
           YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFD YKLHSWGTGL
Sbjct: 481 YFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDAYKLHSWGTGL 540

Query: 541 AEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGET 600
           +EFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGS+LAALEIKAGQMWWQIREGET
Sbjct: 541 SEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSILAALEIKAGQMWWQIREGET 600

Query: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660
           TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR
Sbjct: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660

Query: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720
           ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS
Sbjct: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 720

Query: 721 RGKEEENISNSKIHLV 727
           RGKEE+NI+NSKIHLV
Sbjct: 721 RGKEEQNINNSKIHLV 736

BLAST of MELO3C022070.jh1 vs. ExPASy TrEMBL
Match: A0A6J1BXH2 (Endo-1,3(4)-beta-glucanase OS=Momordica charantia OX=3673 GN=LOC111006593 PE=3 SV=1)

HSP 1 Score: 1281 bits (3314), Expect = 0.0
Identity = 643/736 (87.36%), Postives = 683/736 (92.80%), Query Frame = 0

Query: 1   MFKKIERELRRAFKGKRHS-PPLRLPPPPPPPPPPP-------QTPESQPPEPSQKPSEP 60
           MFKKIERELRRA KGKR S PP RLPPPPPPPPPPP       Q P+S PPEPSQKPSEP
Sbjct: 1   MFKKIERELRRAIKGKRRSSPPQRLPPPPPPPPPPPPPSPPPQQPPKSPPPEPSQKPSEP 60

Query: 61  TPFLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120
           TPFLFP TQSTVLPDPS FFS ELLSAPLP+NSFFQNFTLNNGDQPEYIHPYLIKSSLSS
Sbjct: 61  TPFLFPPTQSTVLPDPSPFFSHELLSAPLPTNSFFQNFTLNNGDQPEYIHPYLIKSSLSS 120

Query: 121 ISVSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRF 180
           ISVSYPSI S  ASE QIFTPDLTI PSEKI PLPQKSHVISSFNDL+VTLDIPSSNLRF
Sbjct: 121 ISVSYPSISSNSASESQIFTPDLTIYPSEKIEPLPQKSHVISSFNDLNVTLDIPSSNLRF 180

Query: 181 YLVRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLT 240
           +LVRGSPFLTFTVSKG AFSIS  HEV+SFSFNNALTKYT+KLKNNQTWLIYSS PINLT
Sbjct: 181 FLVRGSPFLTFTVSKGAAFSISASHEVLSFSFNNALTKYTVKLKNNQTWLIYSSCPINLT 240

Query: 241 HNLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKG 300
           HNLS+ITSGGFAGIIRIAALPNSD ECE ILDRFSSCYPV GEA FTKPFC+EYKWET+G
Sbjct: 241 HNLSVITSGGFAGIIRIAALPNSDPECEPILDRFSSCYPVSGEALFTKPFCMEYKWETRG 300

Query: 301 WGDLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSI 360
           WGDLLMLAHPLH+RLL G++++V+ILDKFKYKSIDG+LVGVVGS W LKPEP+SV WHSI
Sbjct: 301 WGDLLMLAHPLHIRLLAGTNNDVVILDKFKYKSIDGDLVGVVGSLWVLKPEPVSVCWHSI 360

Query: 361 RGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVV 420
           RGVEEESFAEIISALRKDVEALNST+M  TT SPYSYGKLIARAARLAVIAEEVR LEV+
Sbjct: 361 RGVEEESFAEIISALRKDVEALNSTTM--TTTSPYSYGKLIARAARLAVIAEEVRCLEVI 420

Query: 421 PEIRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGY 480
           PEIRKFL+GAIEPWLNGTF+GNGF+YD KWGGIV+KEG+FD  ADFGFGIYNNHHHHLGY
Sbjct: 421 PEIRKFLMGAIEPWLNGTFDGNGFVYDGKWGGIVSKEGSFDSVADFGFGIYNNHHHHLGY 480

Query: 481 FLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRRANSKFPKLRCFDPYKLHSWGTGLA 540
           FLYAIAVLVKIDPAWGRKYSPQ YSLMADIMNLSRR+NSKFP+LRCFD YKLHSWGTGL 
Sbjct: 481 FLYAIAVLVKIDPAWGRKYSPQAYSLMADIMNLSRRSNSKFPRLRCFDVYKLHSWGTGLT 540

Query: 541 EFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGETT 600
           EF DGR QES+SEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQ+REGET 
Sbjct: 541 EFMDGRGQESISEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQVREGET- 600

Query: 601 LYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVRE 660
           LY+EEFVKENR+VGVLWSNKRDSGLWFAP EW+EC+LGIQVLP+LPITELL SDVGFVRE
Sbjct: 601 LYQEEFVKENRLVGVLWSNKRDSGLWFAPREWRECKLGIQVLPVLPITELLFSDVGFVRE 660

Query: 661 LVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHSR 720
           LVNW LPSLGREGVGEGWKGFV+ALESIYDKDG+L+KIRNLK+FDDGNSLTNLLWWVHSR
Sbjct: 661 LVNWTLPSLGREGVGEGWKGFVYALESIYDKDGALEKIRNLKDFDDGNSLTNLLWWVHSR 720

Query: 721 GKE-EENISNSKIHLV 727
           GKE E NIS++K+ LV
Sbjct: 721 GKEGEANISSNKVQLV 733

BLAST of MELO3C022070.jh1 vs. TAIR 10
Match: AT5G15870.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 909.1 bits (2348), Expect = 2.3e-264
Identity = 456/727 (62.72%), Postives = 571/727 (78.54%), Query Frame = 0

Query: 1   MFKKIERELR----RAFKGKRHSPPLRLPPPPPPPPPPPQTPESQPPEPSQKPSEPTPFL 60
           M KK+ R+++    + FK  ++ PP   PPPP P PP P  P SQ    S++ +  TPFL
Sbjct: 1   MLKKVRRKVKVLITKPFKKPKNRPP--SPPPPLPLPPSPSPPPSQQMSSSRQKN--TPFL 60

Query: 61  FPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSISVS 120
           FP + S+VLPDPS FFS +LLS+PLP+NSFFQNFTLNNGDQ EY HPY+IK S SS+S+S
Sbjct: 61  FPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNGDQAEYFHPYIIKPSTSSLSIS 120

Query: 121 YPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYLVR 180
           YPS+    A   + F  D+TI+ S+  +P  +KSH+ISSF+DL VTLD PSSNLRF+LVR
Sbjct: 121 YPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISSFSDLGVTLDFPSSNLRFFLVR 180

Query: 181 GSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTHN-L 240
           GSPF+TF+V+  +  +ISTIH V+S S N + TKYT+KL NNQTWLIY+S PINLT + +
Sbjct: 181 GSPFITFSVNSSI--TISTIHAVLSLSGNTSSTKYTVKLNNNQTWLIYASSPINLTKDGV 240

Query: 241 SMITSG-GFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKGWG 300
           S I  G GF+GIIRI  LPN +   E ILD FS  YPV G+A FTKPF LEYKWE +G+G
Sbjct: 241 SSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVSGDADFTKPFALEYKWEKRGYG 300

Query: 301 DLLMLAHPLHLRLLRGSDDNVIILDKFKYKSIDGELVGVVGSSWALKPEPISVSWHSIRG 360
           DLLMLAHPLHL+LL  +D ++ +LD FKY SIDG+LVGV+G SW LKP+P+SV+WHSI+G
Sbjct: 301 DLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGVIGDSWVLKPDPVSVTWHSIKG 360

Query: 361 VEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKLIARAARLAVIAEEVRSLEVVPE 420
           V+E+S  EIISAL KDV AL+S++ +  T S Y Y KLIARAARLA+IAEEV  L+V+P+
Sbjct: 361 VQEDSHQEIISALIKDVNALDSSAEV--TNSSYFYAKLIARAARLALIAEEVCYLDVIPK 420

Query: 421 IRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAFDHSADFGFGIYNNHHHHLGYFL 480
           IR +L   IEPWLNG+F  NGFLYD KWGG++TK G+ D  ADFGFGIYN+HH+HLGYF+
Sbjct: 421 IRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRDSGADFGFGIYNDHHYHLGYFV 480

Query: 481 YAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSR---RANSKFPKLRCFDPYKLHSWGTGL 540
           YAIAVL KIDP WG++Y PQ Y+LMAD + L +   ++NS +P+LRCFD +KLHSW  GL
Sbjct: 481 YAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSNSNYPRLRCFDLFKLHSWAGGL 540

Query: 541 AEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSIGSMLAALEIKAGQMWWQIREGET 600
            EF DGR+QES SEAVNAYYSAAL+GLAYGD HLV+  SM+  LEI A +MWWQ++E + 
Sbjct: 541 TEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASMVLTLEIHAAKMWWQVKE-DD 600

Query: 601 TLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECRLGIQVLPILPITELLLSDVGFVR 660
            +Y ++F  ENRVVGVLWS KRDSGLWFAP EWKECRLGIQ+LP+LP++E+L SDV FV+
Sbjct: 601 AIYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLLPLLPVSEVLFSDVTFVK 660

Query: 661 ELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQKIRNLKEFDDGNSLTNLLWWVHS 719
           +LVNW +P+L R+ VGEGWKGFV+ALES+YDKDG+++KI+ L  FDDGNSL+NLLWWVHS
Sbjct: 661 QLVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIKGLNGFDDGNSLSNLLWWVHS 718

BLAST of MELO3C022070.jh1 vs. TAIR 10
Match: AT1G18310.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 797.3 bits (2058), Expect = 9.9e-231
Identity = 404/684 (59.06%), Postives = 493/684 (72.08%), Query Frame = 0

Query: 55  FLFPSTQSTVLPDPSVFFSPELLSAPLPSNSFFQNFTLNNGDQPEYIHPYLIKSSLSSIS 114
           FLFP T+S+VLPDPS FFSP+LLS PLP+NSFFQNFTL NGDQ EY HPYLIKS  SS+ 
Sbjct: 8   FLFPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKSVDSSLC 67

Query: 115 VSYPSILSTCASECQIFTPDLTISPSEKINPLPQKSHVISSFNDLSVTLDIPSSNLRFYL 174
           +SYPS+        ++FT D+ IS S    P  +K+H+ISSF+DL VTLD PSSNLRF+L
Sbjct: 68  ISYPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSSNLRFFL 127

Query: 175 VRGSPFLTFTVSKGVAFSISTIHEVISFSFNNALTKYTIKLKNNQTWLIYSSFPINLTH- 234
           VRGSP                          ++ TK++ KL NNQ WLIY+S PI+LT  
Sbjct: 128 VRGSP--------------------------SSSTKWSDKLTNNQRWLIYASSPIDLTKH 187

Query: 235 -NLSMITSGGFAGIIRIAALPNSDLECERILDRFSSCYPVLGEAQFTKPFCLEYKWETKG 294
            + S+   GGF GI+RI  LP S+   E  LDRFSSCYPV G+A FTKPF L+Y WE +G
Sbjct: 188 GDSSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKYMWEKRG 247

Query: 295 WGDLLMLAHPLHLRLL-------------------RG-SDDNVIILDKFKYKSIDGELVG 354
            GDLLMLAHPLHL+LL                   RG ++ +V +LD F+YKSIDG+LVG
Sbjct: 248 SGDLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSIDGDLVG 307

Query: 355 VVGSSWALKPEPISVSWHSIRGVEEESFAEIISALRKDVEALNSTSMILTTKSPYSYGKL 414
           VVG SW LKP+ +SV+WHS++GV+++S+ EIISAL KDV  LNS++ +  T S Y YGKL
Sbjct: 308 VVGDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLNSSAEV--TSSSYFYGKL 367

Query: 415 IARAARLAVIAEEVRSLEVVPEIRKFLIGAIEPWLNGTFEGNGFLYDEKWGGIVTKEGAF 474
           IARAAR A+IAEEV  L+V+P+I  +L   IEPWL+G+F+ NGFLYD KWGG++TK+G+ 
Sbjct: 368 IARAARFALIAEEVCYLDVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGLITKQGSK 427

Query: 475 DHSADFGFGIYNNHHHHLGYFLYAIAVLVKIDPAWGRKYSPQVYSLMADIMNLSRR---- 534
           D  ADFGFGIYN+HH+H+GYFLYAIAVL K DP WG +Y  Q YSL+AD M   R+    
Sbjct: 428 DSQADFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTFGRKDDNN 487

Query: 535 ANSKFPKLRCFDPYKLHSWGTGLAEFTDGRSQESVSEAVNAYYSAALVGLAYGDAHLVSI 594
           +NS +P+LR FD +KLHSW  GL EF DGR+QES SEAVNAYYSAAL+GLAYGD HLV  
Sbjct: 488 SNSSYPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYGDKHLVET 547

Query: 595 GSMLAALEIKAGQMWWQIREGETTLYKEEFVKENRVVGVLWSNKRDSGLWFAPSEWKECR 654
            S +  LEI A +MWWQ+++GE  LY ++F  ENRVVGVLWS KRDS LWF P EWKECR
Sbjct: 548 ASTIMTLEIHAAKMWWQVKKGE-ALYPKDFTAENRVVGVLWSTKRDSSLWFGPKEWKECR 607

Query: 655 LGIQVLPILPITELLLSDVGFVRELVNWALPSLGREGVGEGWKGFVHALESIYDKDGSLQ 713
           LGIQ+LPILP              LVNW LP+L R GVGEGWKGF++ALES+YDKDG+++
Sbjct: 608 LGIQLLPILP--------------LVNWTLPALQRNGVGEGWKGFLYALESLYDKDGAIK 648