MELO3C018511 (gene) Melon (DHL92) v4

Overview


Name	MELO3C018511
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	Amino acid permease family protein
Location	chr01: 765337 .. 768277 (-)
RNA-Seq Expression	MELO3C018511
Synteny	MELO3C018511

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCACATGTATTGACTGAAAAGGAAAAAGAATGTAAAAACTTGGAGCAAGTTGTTCCTTACCCGACGAAAATGGAGAAACGTGGTGGGTTCCTTTCCATTTTTGTCTTCCAAGAAGCACGTCGGATGACAAAGTCAAACAAATCCCAACAATCAACAATTTTCAAATTGAATTTTCTGTGCCAGGTCCCATACATTCACCTAATTACCCGAAGATATTCTACTTCCTATCTTTCCGCTAATTCATATCGACCCAGATTTAGCTTCACTTCTTTCAAATTTCCACTAGATCTTCTTCATATCTCATTCCTGCTTCATCATCGGACTGCTTTTTCTTGCCTATTAAGAACAACCCCTCTTCTCCCCTGAGTCTATGGGGGACGAGAGAAGGGTCGATGCTAAAATTGCTAAAAAACTAACGATTCTTCCTCTGATCGCTTTGATTTTCTACGACGTTTCTGGGGGACCCTTTGGAGTGGAGGATTCGGTGAGCACTGGCGGAGGTCCGCTTCTGGCTTTGTTGGGTTTTTTAGTGTTCCCATTTATTTGGAGCATTCCGGAGGCTTTGGTCACGGCGGAGCTCGCCACGATTTTCCCTCAAAATGGCGGGTATGTGATCTGGATTTCGGCTGCTTTTGGCCCTTTTTGGGGTTTTCAAGAAGGGTTTTGGAAATGGTTCAGTGGGGCAATGGATAATGCTTTGTATCCTGTTCTGTTTCTTGATTACTTGAAACGTTCGTTTCCTGTTTTTAACCATATATTTGCTCGAATTCCAGCTTTATTAGGAATCACTGCTTCTTTAACTTACTTAAACTATCGTGGTCTCCACATTGTTGGGGTTTCTGCTGTTGTTCTTGCTGTGTTCTCGCTTTGCCCCTTTGTGGTGATGACCCTTCTTTCAATTCCTAGAATAAGCCCCAAAAAGTGGTTAATTCTAGAGTATAGCAAGGTAAATTGGAGGGGTTATTTCAATAGTATGTTTTGGAATTTGAACTATTGGGATAAAGCAAGTACTTTAGCAGGGGAGGTTGAAAATCCCAGTAAAACTTTCCCTAGAGCTATGTTTGGAGCTGTGGTTTTGGTGGTTTCCTCTTATTTGATTCCTCTTCTAGCTGGGACTGGTGCCTTGGCAACAGATTCAAGCGAATGGAGTGATGGGTATTTTGCAGAGGTTGGGGCTTTGATTGGTGGGGTTTGGCTGAAGTGGTGGATTCAAGCTGCTGCTGCTATGTCTAACATGGGGTTGTTTGAGGCTGAAATGAGCAGTGATGCATATCAACTGCTTGGAATGAGTGAGATGGGAATGATTCCTTCTGTGTTTGCTTCAAGGTTAGTGGTTCCTTTTCTGTTTCCTTCCATTGTAGACTGTATTGGTTGCAATTTTGTAGCTCAAATTGGTAGTAGTGATCCAAAATAGCAACCTTTGGGGAGGTCAAAGGTTTAAATTACCATTTTTATATAATCGAAGGTAGCTTAAATGGGTCGGGATAACAATATGCATTTTAGTAATAAGTCAACATTGACATAACTCATAGCCATAAAGAGGATGATAGAATGAGGATGATTGCAAAAGATTGATAGTCTGAAAGTAGAATCCTTTAAACTATAAGACTCATAGTGGTATTTTTAGCTTCAAAGATGAGTTCGTATAGTATAGCTGAAGATTATAAGATACATTTTTGTTAATCCCATGTACTAATTCAAGGTCAAATTGCAAATTTGGTTTGTATGGTTTGGTAAAAGTTAGAATCGATTCCCTGGTATATCGTTAGAATTTTGTCTCTATTGTTTGATAGAAACTTTCACAATAATTCCTTCTATGGGGATATAAAATAATTTTATCAAACTATAGGAACTATTTAAGAGGTTTTATCAAGACTAAATGCTAACTTTTGAAATAATAGGAATTAAACTCTAGCTTAATTCAAAGTTTGATACCTTTCTACTACCTGTGCCCATCTTCAAGTCCCGGATCTATACAAACTCAAACTTTTCGATCTCAGACTTGTAAAACCTTAAGCCAAACATTTCCTAAACTCTTCCAGCATTTTGATCCATTTTCTTTACTGGCTCATTGTGGATGCAAAAGCTGATCTTATCGAACTATTGTCCCTTGTTCACTTTGCAGATCCAAATATGGGACACCCACCTTCAGCATTCTGTGCTCTGCCGTAGGAGTTATCTTCCTTTCATGGATGAGTTTCCAAGAAATACTTGAGTTTCTGAACTTCCTATACTCTGTAGGAATGCTTTTAGAGTTTGCTGCCTTTATTAAACTAAGAATAAGAAAGCCAGATCTCAATAGACCCTACAAAGTTCCCTTGCAAACAGTTGGTGTCACATTGCTTTGCTTCCCACCTTCTGCCTTGCTTATTCTCGTCATGTGTTTAGCTTCTGCAAAAACATTCTTAATCAGTGGGATTATTATTGCAGTGGGGTTTCTTCTATATCCTACTCTTTTGCAAGCAAAGAATAGAAGATGGGTTAAATTTATCTCGGAACAGCCAGAAGATACTACTCTTTCTGATGTGGAAGATAGGCTGGTTGAACCGCAACCGCAGCAAGAAGTTCCCAACGAGGCAGAGGTTCGGCTTCTTTCAGAATCTTCGTCTTCGAATATAGCCCAGCAATAACATCCATAGATTACCTGATTTCAACGCAATCAACCTATGTGCTGGCCATGCTGTTTCAAAGCTTACTCGTAAGGGAAATTTCACTTCTGCAATGTTCTGAGAAGATAATGTGCGTGATCGATGAAATATTGAAGTTGCATGTTGTCATGTAAACATATTTATGTAGAAAATTGTTTTTATATTCTTTTGGAAGGTTGATATGACGTCAACGGTTGTAACACAAAAGAAAGGGTTAGTTTTCTGCAATATTATCATTTTAATTAGAATCAAATAAAGCTTAAAGCGTTTGGAATTTTCTTTTCAATTACAAA

mRNA sequence

TCACATGTATTGACTGAAAAGGAAAAAGAATGTAAAAACTTGGAGCAAGTTGTTCCTTACCCGACGAAAATGGAGAAACGTGGTGGGTTCCTTTCCATTTTTGTCTTCCAAGAAGCACGTCGGATGACAAAGTCAAACAAATCCCAACAATCAACAATTTTCAAATTGAATTTTCTGTGCCAGGTCCCATACATTCACCTAATTACCCGAAGATATTCTACTTCCTATCTTTCCGCTAATTCATATCGACCCAGATTTAGCTTCACTTCTTTCAAATTTCCACTAGATCTTCTTCATATCTCATTCCTGCTTCATCATCGGACTGCTTTTTCTTGCCTATTAAGAACAACCCCTCTTCTCCCCTGAGTCTATGGGGGACGAGAGAAGGGTCGATGCTAAAATTGCTAAAAAACTAACGATTCTTCCTCTGATCGCTTTGATTTTCTACGACGTTTCTGGGGGACCCTTTGGAGTGGAGGATTCGGTGAGCACTGGCGGAGGTCCGCTTCTGGCTTTGTTGGGTTTTTTAGTGTTCCCATTTATTTGGAGCATTCCGGAGGCTTTGGTCACGGCGGAGCTCGCCACGATTTTCCCTCAAAATGGCGGGTATGTGATCTGGATTTCGGCTGCTTTTGGCCCTTTTTGGGGTTTTCAAGAAGGGTTTTGGAAATGGTTCAGTGGGGCAATGGATAATGCTTTGTATCCTGTTCTGTTTCTTGATTACTTGAAACGTTCGTTTCCTGTTTTTAACCATATATTTGCTCGAATTCCAGCTTTATTAGGAATCACTGCTTCTTTAACTTACTTAAACTATCGTGGTCTCCACATTGTTGGGGTTTCTGCTGTTGTTCTTGCTGTGTTCTCGCTTTGCCCCTTTGTGGTGATGACCCTTCTTTCAATTCCTAGAATAAGCCCCAAAAAGTGGTTAATTCTAGAGTATAGCAAGGTAAATTGGAGGGGTTATTTCAATAGTATGTTTTGGAATTTGAACTATTGGGATAAAGCAAGTACTTTAGCAGGGGAGGTTGAAAATCCCAGTAAAACTTTCCCTAGAGCTATGTTTGGAGCTGTGGTTTTGGTGGTTTCCTCTTATTTGATTCCTCTTCTAGCTGGGACTGGTGCCTTGGCAACAGATTCAAGCGAATGGAGTGATGGGTATTTTGCAGAGGTTGGGGCTTTGATTGGTGGGGTTTGGCTGAAGTGGTGGATTCAAGCTGCTGCTGCTATGTCTAACATGGGGTTGTTTGAGGCTGAAATGAGCAGTGATGCATATCAACTGCTTGGAATGAGTGAGATGGGAATGATTCCTTCTGTGTTTGCTTCAAGATCCAAATATGGGACACCCACCTTCAGCATTCTGTGCTCTGCCGTAGGAGTTATCTTCCTTTCATGGATGAGTTTCCAAGAAATACTTGAGTTTCTGAACTTCCTATACTCTGTAGGAATGCTTTTAGAGTTTGCTGCCTTTATTAAACTAAGAATAAGAAAGCCAGATCTCAATAGACCCTACAAAGTTCCCTTGCAAACAGTTGGTGTCACATTGCTTTGCTTCCCACCTTCTGCCTTGCTTATTCTCGTCATGTGTTTAGCTTCTGCAAAAACATTCTTAATCAGTGGGATTATTATTGCAGTGGGGTTTCTTCTATATCCTACTCTTTTGCAAGCAAAGAATAGAAGATGGGTTAAATTTATCTCGGAACAGCCAGAAGATACTACTCTTTCTGATGTGGAAGATAGGCTGGTTGAACCGCAACCGCAGCAAGAAGTTCCCAACGAGGCAGAGGTTCGGCTTCTTTCAGAATCTTCGTCTTCGAATATAGCCCAGCAATAACATCCATAGATTACCTGATTTCAACGCAATCAACCTATGTGCTGGCCATGCTGTTTCAAAGCTTACTCGTAAGGGAAATTTCACTTCTGCAATGTTCTGAGAAGATAATGTGCGTGATCGATGAAATATTGAAGTTGCATGTTGTCATGTAAACATATTTATGTAGAAAATTGTTTTTATATTCTTTTGGAAGGTTGATATGACGTCAACGGTTGTAACACAAAAGAAAGGGTTAGTTTTCTGCAATATTATCATTTTAATTAGAATCAAATAAAGCTTAAAGCGTTTGGAATTTTCTTTTCAATTACAAA

Coding sequence (CDS)

ATGGGGGACGAGAGAAGGGTCGATGCTAAAATTGCTAAAAAACTAACGATTCTTCCTCTGATCGCTTTGATTTTCTACGACGTTTCTGGGGGACCCTTTGGAGTGGAGGATTCGGTGAGCACTGGCGGAGGTCCGCTTCTGGCTTTGTTGGGTTTTTTAGTGTTCCCATTTATTTGGAGCATTCCGGAGGCTTTGGTCACGGCGGAGCTCGCCACGATTTTCCCTCAAAATGGCGGGTATGTGATCTGGATTTCGGCTGCTTTTGGCCCTTTTTGGGGTTTTCAAGAAGGGTTTTGGAAATGGTTCAGTGGGGCAATGGATAATGCTTTGTATCCTGTTCTGTTTCTTGATTACTTGAAACGTTCGTTTCCTGTTTTTAACCATATATTTGCTCGAATTCCAGCTTTATTAGGAATCACTGCTTCTTTAACTTACTTAAACTATCGTGGTCTCCACATTGTTGGGGTTTCTGCTGTTGTTCTTGCTGTGTTCTCGCTTTGCCCCTTTGTGGTGATGACCCTTCTTTCAATTCCTAGAATAAGCCCCAAAAAGTGGTTAATTCTAGAGTATAGCAAGGTAAATTGGAGGGGTTATTTCAATAGTATGTTTTGGAATTTGAACTATTGGGATAAAGCAAGTACTTTAGCAGGGGAGGTTGAAAATCCCAGTAAAACTTTCCCTAGAGCTATGTTTGGAGCTGTGGTTTTGGTGGTTTCCTCTTATTTGATTCCTCTTCTAGCTGGGACTGGTGCCTTGGCAACAGATTCAAGCGAATGGAGTGATGGGTATTTTGCAGAGGTTGGGGCTTTGATTGGTGGGGTTTGGCTGAAGTGGTGGATTCAAGCTGCTGCTGCTATGTCTAACATGGGGTTGTTTGAGGCTGAAATGAGCAGTGATGCATATCAACTGCTTGGAATGAGTGAGATGGGAATGATTCCTTCTGTGTTTGCTTCAAGATCCAAATATGGGACACCCACCTTCAGCATTCTGTGCTCTGCCGTAGGAGTTATCTTCCTTTCATGGATGAGTTTCCAAGAAATACTTGAGTTTCTGAACTTCCTATACTCTGTAGGAATGCTTTTAGAGTTTGCTGCCTTTATTAAACTAAGAATAAGAAAGCCAGATCTCAATAGACCCTACAAAGTTCCCTTGCAAACAGTTGGTGTCACATTGCTTTGCTTCCCACCTTCTGCCTTGCTTATTCTCGTCATGTGTTTAGCTTCTGCAAAAACATTCTTAATCAGTGGGATTATTATTGCAGTGGGGTTTCTTCTATATCCTACTCTTTTGCAAGCAAAGAATAGAAGATGGGTTAAATTTATCTCGGAACAGCCAGAAGATACTACTCTTTCTGATGTGGAAGATAGGCTGGTTGAACCGCAACCGCAGCAAGAAGTTCCCAACGAGGCAGAGGTTCGGCTTCTTTCAGAATCTTCGTCTTCGAATATAGCCCAGCAATAA

Protein sequence

MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWSIPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRISPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSSYLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIAVGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSSSNIAQQ

Homology

[Close]

BLAST of MELO3C018511 vs. NCBI nr
Match: XP_008455023.1 (PREDICTED: probable polyamine transporter At3g19553 isoform X1 [Cucumis melo])

HSP 1 Score: 951.0 bits (2457), Expect = 3.9e-273
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 0


Query: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60
           MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240
           SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420
           LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480
           VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480

Query: 481 SNIAQQ 487
           SNIAQQ
Sbjct: 481 SNIAQQ 486

[Close]

BLAST of MELO3C018511 vs. NCBI nr
Match: KAA0031359.1 (putative polyamine transporter [Cucumis melo var. makuwa] >TYK06811.1 putative polyamine transporter [Cucumis melo var. makuwa])

HSP 1 Score: 949.9 bits (2454), Expect = 8.6e-273
Identity = 485/486 (99.79%), Postives = 486/486 (100.00%), Query Frame = 0


Query: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60
           MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240
           SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFP+AMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420
           LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480
           VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480

Query: 481 SNIAQQ 487
           SNIAQQ
Sbjct: 481 SNIAQQ 486

[Close]

BLAST of MELO3C018511 vs. NCBI nr
Match: XP_004136943.1 (probable polyamine transporter At3g19553 [Cucumis sativus] >KGN43858.1 hypothetical protein Csa_017296 [Cucumis sativus])

HSP 1 Score: 924.9 bits (2389), Expect = 3.0e-265
Identity = 469/486 (96.50%), Postives = 478/486 (98.35%), Query Frame = 0


Query: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60
           MGDERRVDAKI +KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS
Sbjct: 1   MGDERRVDAKITQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240
           SPKKWL++EYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFP+AMFGAVVLVVS 
Sbjct: 181 SPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSF 240

Query: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL TDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALETDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGM+PSVFASRSKYGTPTFSILCSA+GVIFLSWMSFQEILEFLNFLY++GML
Sbjct: 301 YQLLGMSEMGMLPSVFASRSKYGTPTFSILCSALGVIFLSWMSFQEILEFLNFLYAIGML 360

Query: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420
           LEFAAFIKLRIRKPDLNRPYKVPLQT GVTLLCFPP+ALL LVMCLASAKTFLISGIIIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTFGVTLLCFPPAALLFLVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480
           VGFLLYPTLLQAKNRRWVKFISEQPEDTTL DVEDRLVE Q QQEVPNEAEVRLLSESSS
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLPDVEDRLVESQQQQEVPNEAEVRLLSESSS 480

Query: 481 SNIAQQ 487
           SNIAQQ
Sbjct: 481 SNIAQQ 486

[Close]

BLAST of MELO3C018511 vs. NCBI nr
Match: XP_038888395.1 (probable polyamine transporter At3g19553 [Benincasa hispida])

HSP 1 Score: 896.0 bits (2314), Expect = 1.5e-256
Identity = 454/487 (93.22%), Postives = 471/487 (96.71%), Query Frame = 0


Query: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60
           MGDERR DAKIA+KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS
Sbjct: 1   MGDERRADAKIAQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNH+FARIP LLGITASLTYLNYRGLHIVGVSAVVLA FSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHVFARIPTLLGITASLTYLNYRGLHIVGVSAVVLAAFSLCPFVVMTLLSIPRI 180

Query: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240
            PKKWL++EYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFP+AMFGAVVLVVSS
Sbjct: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL +DSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGM+PSVFA+RSKYGTPTFSILCSA+GVIFLSWMSFQEILEFLNFLYSVGML
Sbjct: 301 YQLLGMSEMGMLPSVFAARSKYGTPTFSILCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420
           LEFAAFIKLRI+KPDLNRPY+VPLQT GVT+LCFPP+ALL+LVMCLASAKTFLISGII+ 
Sbjct: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVT 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSE-SS 480
           VGFLLYPTLLQAKN+RWVKFISEQPE  TL DVEDRLVE   QQEVPNEAEVRLLSE SS
Sbjct: 421 VGFLLYPTLLQAKNKRWVKFISEQPE-ATLPDVEDRLVESHAQQEVPNEAEVRLLSESSS 480

Query: 481 SSNIAQQ 487
           SSNIAQQ
Sbjct: 481 SSNIAQQ 486

[Close]

BLAST of MELO3C018511 vs. NCBI nr
Match: XP_022972448.1 (probable polyamine transporter At3g19553 [Cucurbita maxima])

HSP 1 Score: 850.5 bits (2196), Expect = 7.1e-243
Identity = 435/487 (89.32%), Postives = 458/487 (94.05%), Query Frame = 0


Query: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60
           MGDER   AKIA KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS
Sbjct: 1   MGDER---AKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVF+HIFARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSLCPFVVMT+LSIPRI
Sbjct: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180

Query: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240
            P+KWL+++YS VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFP+AMFGAVVLVV S
Sbjct: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240

Query: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGAL TDSS+WSDGYFAEVGALIGG WLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLVPLLAGTGALETDSSDWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA+GVIFLSWMSFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420
           LE AAFIKLRI+KPDL+RPYKVPLQT G  +LC PP+ALL+LVMCLASA+TFLISGIII+
Sbjct: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGIIIS 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSES-S 480
           VGFLLYP+L QAKNR WVKFISEQ  D TL DVED LVEPQP+QEV +EA +RLLSES S
Sbjct: 421 VGFLLYPSLSQAKNRGWVKFISEQQGD-TLPDVEDCLVEPQPRQEVLDEAVIRLLSESPS 480

Query: 481 SSNIAQQ 487
           S NIA Q
Sbjct: 481 SPNIANQ 483

[Close]

BLAST of MELO3C018511 vs. ExPASy Swiss-Prot
Match: Q9LH39 (Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g19553 PE=3 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 9.7e-203
Identity = 346/460 (75.22%), Postives = 399/460 (86.74%), Query Frame = 0


Query: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60
           + DE     K + KLT+LPL+ LIFY+VSGGPFGVEDSV +GGGPLLALLGFL+FP IWS
Sbjct: 8   VNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWS 67

Query: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELAT FP+NGGYV+WIS+AFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 68  IPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 127

Query: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
            SFPV +H+ AR+PALL IT SLTYLNYRGLHIVG SAVVLAVFSLCPFVVM LL++P I
Sbjct: 128 HSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNI 187

Query: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240
            PK+WL ++  K+NWRGYFN+MFWNLNYWDKASTLAGEV+ P KTFP+A+FGAV+LV+ S
Sbjct: 188 RPKRWLFVDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGS 247

Query: 241 YLIPLLAGTGALATDSS-EWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSD 300
           YLIPL+AGTGAL++ +S EWSDGYFAEVG LIGGVWLK WIQAAAAMSN+GLFEAEMSSD
Sbjct: 248 YLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSD 307

Query: 301 AYQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGM 360
           A+QLLGMSE+GM+P+ FA RSKYGTPT SILCSA GVIFLSWMSFQEI+EFLNFLY++GM
Sbjct: 308 AFQLLGMSEIGMLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGM 367

Query: 361 LLEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIII 420
           LLEFAAF+KLRI+KPDL+RPY+VPL T GV++LC PPS L+ILVM LA+ KTFLISG+II
Sbjct: 368 LLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVII 427

Query: 421 AVGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVE 460
            +GF LYP L   K ++W +FI E+    +    E +L E
Sbjct: 428 VLGFCLYPFLTLVKEKQWARFIPEETRPVSGVSSESQLDE 467

[Close]

BLAST of MELO3C018511 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 5.1e-151
Identity = 254/430 (59.07%), Postives = 332/430 (77.21%), Query Frame = 0


Query: 13  KKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWSIPEALVTAELAT 72
           KK+T+LPL+ LIFY+VSGGPFG+EDSV    GPLLA++GF+VFPFIWSIPEAL+TAE+ T
Sbjct: 51  KKITVLPLVFLIFYEVSGGPFGIEDSVK-AAGPLLAIVGFIVFPFIWSIPEALITAEMGT 110

Query: 73  IFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVFNHIFAR 132
           +FP+NGGYV+W++ A GP+WGFQ+G+ KW SG +DNALYP+LFLDYLK   P+      R
Sbjct: 111 MFPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPR 170

Query: 133 IPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRISPKKWLIL--EY 192
           + A+L +T +LTYLNYRGL IVGV+AV+L VFS+ PFVVM+ +SIP++ P +WL++  + 
Sbjct: 171 VAAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKM 230

Query: 193 SKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSSYLIPLLAGTG 252
             VNW  Y N++FWNLNYWD  STL GEVENPSKT PRA+F A++LVV SY+ P+L GTG
Sbjct: 231 KGVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTG 290

Query: 253 ALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMG 312
           A+A D   W+DGYFA++G +IGGVWL WWIQAAAA SNMG+F AEMSSD++QLLGM+E G
Sbjct: 291 AIALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERG 350

Query: 313 MIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLR 372
           M+P VFA RS+Y TP   IL SA GVI LSW+SFQEI+   N LY  GM+LEF  F++LR
Sbjct: 351 MLPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLR 410

Query: 373 IRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIAVGFLLYPTLL 432
           ++ P  +RP+K+P+  +G  L+C PP+ L+ ++M   + K  L+S   I +G +L P L 
Sbjct: 411 MKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLK 470

Query: 433 QAKNRRWVKF 441
           Q + + W+KF
Sbjct: 471 QVEKKGWLKF 479

[Close]

BLAST of MELO3C018511 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 8.6e-151
Identity = 249/428 (58.18%), Postives = 330/428 (77.10%), Query Frame = 0


Query: 13  KKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWSIPEALVTAELAT 72
           +K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGF++FPFIWSIPEAL+TAE+ T
Sbjct: 47  RKVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGT 106

Query: 73  IFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVFNHIFAR 132
           ++P+NGGYV+W+S+A GPFWGFQ+G+ KW SG +DNALYPVLFLDYLK   P       R
Sbjct: 107 MYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPR 166

Query: 133 IPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRISPKKWLILEYSK 192
           + ++L +T  LTYLNYRGL IVG  AV++ VFS+ PF VM L+SIP++ P +WL+++   
Sbjct: 167 VASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGN 226

Query: 193 VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSSYLIPLLAGTGAL 252
           VNW  Y N++FWNLNYWD  STLAGEVENP+ T P+A+F  V+LV  SY+ PLLAG GA+
Sbjct: 227 VNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAI 286

Query: 253 ATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMI 312
             +  +W+DGYF++V   +GG WL+WW+QAAAA SNMG+F AEMSSD++QLLGM+E GM+
Sbjct: 287 PLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGML 346

Query: 313 PSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRIR 372
           P  FA RS+YGTP   IL SA GV+ LSW+SFQEI+   N LY VGM+LEF AF+++R++
Sbjct: 347 PEFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMK 406

Query: 373 KPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIAVGFLLYPTLLQA 432
            P  +RPYK+P+ T G  L+C PP+ L+  V+ L+S K   +S +++ +GFL++P L   
Sbjct: 407 HPAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHM 466

Query: 433 KNRRWVKF 441
             +RWVKF
Sbjct: 467 DRKRWVKF 473

[Close]

BLAST of MELO3C018511 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.1e-145
Identity = 249/429 (58.04%), Postives = 327/429 (76.22%), Query Frame = 0


Query: 12  AKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWSIPEALVTAELA 71
           A+ ++++PLI LIFY+VSGGPFG+EDSV    GPLLA++GFLV P IWSIPEAL+TAEL 
Sbjct: 80  ARTVSMIPLIFLIFYEVSGGPFGIEDSVG-AAGPLLAIIGFLVLPVIWSIPEALITAELG 139

Query: 72  TIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVFNHIFA 131
            +FP+NGGYV+W+++A GP+WGFQ+G+ KW SG +DNALYPVLFLDYLK   P       
Sbjct: 140 AMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGGGAP 199

Query: 132 RIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRISPKKWLILEYS 191
           R  A++G+TA LT LNYRGL +VG  A+ L VFSL PF VM L+++P++ P +WL+++  
Sbjct: 200 RAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLRPARWLVIDLH 259

Query: 192 KVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSSYLIPLLAGTGA 251
            V+W  Y N++FWNLNYWD  STLAGEV+NP KT P+A+F AV+ VV +YL PLLAGTGA
Sbjct: 260 NVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAYLYPLLAGTGA 319

Query: 252 LATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGM 311
           +  D  +W+DGYFA++  L+GG WL WW+Q+AAA+SNMG+F AEMSSD+YQLLGM+E GM
Sbjct: 320 VPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSYQLLGMAERGM 379

Query: 312 IPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRI 371
           +PS FA+RS+YGTP   IL SA GV+ LS MSFQEI+   NFLY  GMLLEF AFI  R+
Sbjct: 380 LPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLLEFVAFILHRV 439

Query: 372 RKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIAVGFLLYPTLLQ 431
           R+PD  RPY+VPL T G   +  PP+AL+ +V+ L++ K  ++S   +A+G +L P L  
Sbjct: 440 RRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAMGLVLQPALRF 499

Query: 432 AKNRRWVKF 441
            + +RW++F
Sbjct: 500 VEKKRWLRF 507

[Close]

BLAST of MELO3C018511 vs. ExPASy Swiss-Prot
Match: Q6Z8D0 (Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.1e-145
Identity = 249/429 (58.04%), Postives = 327/429 (76.22%), Query Frame = 0


Query: 12  AKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWSIPEALVTAELA 71
           A+ ++++PLI LIFY+VSGGPFG+EDSV    GPLLA++GFLV P IWSIPEAL+TAEL 
Sbjct: 80  ARTVSMIPLIFLIFYEVSGGPFGIEDSVG-AAGPLLAIIGFLVLPVIWSIPEALITAELG 139

Query: 72  TIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVFNHIFA 131
            +FP+NGGYV+W+++A GP+WGFQ+G+ KW SG +DNALYPVLFLDYLK   P       
Sbjct: 140 AMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGGGAP 199

Query: 132 RIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRISPKKWLILEYS 191
           R  A++G+TA LT LNYRGL +VG  A+ L VFSL PF VM L+++P++ P +WL+++  
Sbjct: 200 RAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLRPARWLVIDLH 259

Query: 192 KVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSSYLIPLLAGTGA 251
            V+W  Y N++FWNLNYWD  STLAGEV+NP KT P+A+F AV+ VV +YL PLLAGTGA
Sbjct: 260 NVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAYLYPLLAGTGA 319

Query: 252 LATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGM 311
           +  D  +W+DGYFA++  L+GG WL WW+Q+AAA+SNMG+F AEMSSD+YQLLGM+E GM
Sbjct: 320 VPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSYQLLGMAERGM 379

Query: 312 IPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRI 371
           +PS FA+RS+YGTP   IL SA GV+ LS MSFQEI+   NFLY  GMLLEF AFI  R+
Sbjct: 380 LPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLLEFVAFILHRV 439

Query: 372 RKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIAVGFLLYPTLLQ 431
           R+PD  RPY+VPL T G   +  PP+AL+ +V+ L++ K  ++S   +A+G +L P L  
Sbjct: 440 RRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAMGLVLQPALRF 499

Query: 432 AKNRRWVKF 441
            + +RW++F
Sbjct: 500 VEKKRWLRF 507

[Close]

BLAST of MELO3C018511 vs. ExPASy TrEMBL
Match: A0A1S3C014 (probable polyamine transporter At3g19553 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495296 PE=3 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 1.9e-273
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 0


Query: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60
           MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240
           SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420
           LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480
           VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480

Query: 481 SNIAQQ 487
           SNIAQQ
Sbjct: 481 SNIAQQ 486

[Close]

BLAST of MELO3C018511 vs. ExPASy TrEMBL
Match: A0A5D3C4H2 (Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001140 PE=3 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 4.2e-273
Identity = 485/486 (99.79%), Postives = 486/486 (100.00%), Query Frame = 0


Query: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60
           MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240
           SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFP+AMFGAVVLVVSS
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420
           LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480
           VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480

Query: 481 SNIAQQ 487
           SNIAQQ
Sbjct: 481 SNIAQQ 486

[Close]

BLAST of MELO3C018511 vs. ExPASy TrEMBL
Match: A0A0A0K2Y2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071470 PE=3 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 1.4e-265
Identity = 469/486 (96.50%), Postives = 478/486 (98.35%), Query Frame = 0


Query: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60
           MGDERRVDAKI +KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS
Sbjct: 1   MGDERRVDAKITQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240
           SPKKWL++EYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFP+AMFGAVVLVVS 
Sbjct: 181 SPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSF 240

Query: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YLIPLLAGTGAL TDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALETDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGM+PSVFASRSKYGTPTFSILCSA+GVIFLSWMSFQEILEFLNFLY++GML
Sbjct: 301 YQLLGMSEMGMLPSVFASRSKYGTPTFSILCSALGVIFLSWMSFQEILEFLNFLYAIGML 360

Query: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420
           LEFAAFIKLRIRKPDLNRPYKVPLQT GVTLLCFPP+ALL LVMCLASAKTFLISGIIIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTFGVTLLCFPPAALLFLVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSESSS 480
           VGFLLYPTLLQAKNRRWVKFISEQPEDTTL DVEDRLVE Q QQEVPNEAEVRLLSESSS
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLPDVEDRLVESQQQQEVPNEAEVRLLSESSS 480

Query: 481 SNIAQQ 487
           SNIAQQ
Sbjct: 481 SNIAQQ 486

[Close]

BLAST of MELO3C018511 vs. ExPASy TrEMBL
Match: A0A6J1I9Z5 (probable polyamine transporter At3g19553 OS=Cucurbita maxima OX=3661 GN=LOC111471008 PE=3 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 3.4e-243
Identity = 435/487 (89.32%), Postives = 458/487 (94.05%), Query Frame = 0


Query: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60
           MGDER   AKIA KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS
Sbjct: 1   MGDER---AKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVF+HIFARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSLCPFVVMT+LSIPRI
Sbjct: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180

Query: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240
            P+KWL+++YS VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFP+AMFGAVVLVV S
Sbjct: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240

Query: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGAL TDSS+WSDGYFAEVGALIGG WLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLVPLLAGTGALETDSSDWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA+GVIFLSWMSFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420
           LE AAFIKLRI+KPDL+RPYKVPLQT G  +LC PP+ALL+LVMCLASA+TFLISGIII+
Sbjct: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGIIIS 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSES-S 480
           VGFLLYP+L QAKNR WVKFISEQ  D TL DVED LVEPQP+QEV +EA +RLLSES S
Sbjct: 421 VGFLLYPSLSQAKNRGWVKFISEQQGD-TLPDVEDCLVEPQPRQEVLDEAVIRLLSESPS 480

Query: 481 SSNIAQQ 487
           S NIA Q
Sbjct: 481 SPNIANQ 483

[Close]

BLAST of MELO3C018511 vs. ExPASy TrEMBL
Match: A0A6J1GKM4 (probable polyamine transporter At3g19553 OS=Cucurbita moschata OX=3662 GN=LOC111455227 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 5.0e-242
Identity = 432/487 (88.71%), Postives = 456/487 (93.63%), Query Frame = 0


Query: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60
           MGDER   A I  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDER---ANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWS 60

Query: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
           RSFPVF+HIFARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSLCPFVVMT+LSIPRI
Sbjct: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180

Query: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240
            P+KWL+++YS VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFP+AMFGAVVLVV S
Sbjct: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240

Query: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGAL TDSSEWSDGYFAEVGALIGG WLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLVPLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA+GVIFLSWMSFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360

Query: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420
           LE AAFIKLRI+KPDL+RPYKVPLQT G  +LC PP+ALL+LVMCLASA+TFLISG+IIA
Sbjct: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIA 420

Query: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSES-S 480
           VGFLLYP+L QAKNR WVKFISEQ  D TL DVED LVEPQP+QEV +E+ +RLLSES S
Sbjct: 421 VGFLLYPSLSQAKNRGWVKFISEQQGD-TLPDVEDCLVEPQPRQEVLDESVIRLLSESPS 480

Query: 481 SSNIAQQ 487
           S NIA Q
Sbjct: 481 SPNIANQ 483

[Close]

BLAST of MELO3C018511 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 707.6 bits (1825), Expect = 6.9e-204
Identity = 346/460 (75.22%), Postives = 399/460 (86.74%), Query Frame = 0


Query: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60
           + DE     K + KLT+LPL+ LIFY+VSGGPFGVEDSV +GGGPLLALLGFL+FP IWS
Sbjct: 8   VNDENSSKPKPSPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWS 67

Query: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120
           IPEALVTAELAT FP+NGGYV+WIS+AFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 68  IPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 127

Query: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180
            SFPV +H+ AR+PALL IT SLTYLNYRGLHIVG SAVVLAVFSLCPFVVM LL++P I
Sbjct: 128 HSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNI 187

Query: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240
            PK+WL ++  K+NWRGYFN+MFWNLNYWDKASTLAGEV+ P KTFP+A+FGAV+LV+ S
Sbjct: 188 RPKRWLFVDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGS 247

Query: 241 YLIPLLAGTGALATDSS-EWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSD 300
           YLIPL+AGTGAL++ +S EWSDGYFAEVG LIGGVWLK WIQAAAAMSN+GLFEAEMSSD
Sbjct: 248 YLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSD 307

Query: 301 AYQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGM 360
           A+QLLGMSE+GM+P+ FA RSKYGTPT SILCSA GVIFLSWMSFQEI+EFLNFLY++GM
Sbjct: 308 AFQLLGMSEIGMLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGM 367

Query: 361 LLEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIII 420
           LLEFAAF+KLRI+KPDL+RPY+VPL T GV++LC PPS L+ILVM LA+ KTFLISG+II
Sbjct: 368 LLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVII 427

Query: 421 AVGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVE 460
            +GF LYP L   K ++W +FI E+    +    E +L E
Sbjct: 428 VLGFCLYPFLTLVKEKQWARFIPEETRPVSGVSSESQLDE 467

[Close]

BLAST of MELO3C018511 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 535.8 bits (1379), Expect = 3.6e-152
Identity = 254/430 (59.07%), Postives = 332/430 (77.21%), Query Frame = 0


Query: 13  KKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWSIPEALVTAELAT 72
           KK+T+LPL+ LIFY+VSGGPFG+EDSV    GPLLA++GF+VFPFIWSIPEAL+TAE+ T
Sbjct: 51  KKITVLPLVFLIFYEVSGGPFGIEDSVK-AAGPLLAIVGFIVFPFIWSIPEALITAEMGT 110

Query: 73  IFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVFNHIFAR 132
           +FP+NGGYV+W++ A GP+WGFQ+G+ KW SG +DNALYP+LFLDYLK   P+      R
Sbjct: 111 MFPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPR 170

Query: 133 IPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRISPKKWLIL--EY 192
           + A+L +T +LTYLNYRGL IVGV+AV+L VFS+ PFVVM+ +SIP++ P +WL++  + 
Sbjct: 171 VAAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKM 230

Query: 193 SKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSSYLIPLLAGTG 252
             VNW  Y N++FWNLNYWD  STL GEVENPSKT PRA+F A++LVV SY+ P+L GTG
Sbjct: 231 KGVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTG 290

Query: 253 ALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMG 312
           A+A D   W+DGYFA++G +IGGVWL WWIQAAAA SNMG+F AEMSSD++QLLGM+E G
Sbjct: 291 AIALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERG 350

Query: 313 MIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLR 372
           M+P VFA RS+Y TP   IL SA GVI LSW+SFQEI+   N LY  GM+LEF  F++LR
Sbjct: 351 MLPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLR 410

Query: 373 IRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIAVGFLLYPTLL 432
           ++ P  +RP+K+P+  +G  L+C PP+ L+ ++M   + K  L+S   I +G +L P L 
Sbjct: 411 MKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLK 470

Query: 433 QAKNRRWVKF 441
           Q + + W+KF
Sbjct: 471 QVEKKGWLKF 479

[Close]

BLAST of MELO3C018511 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 535.0 bits (1377), Expect = 6.1e-152
Identity = 249/428 (58.18%), Postives = 330/428 (77.10%), Query Frame = 0


Query: 13  KKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWSIPEALVTAELAT 72
           +K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGF++FPFIWSIPEAL+TAE+ T
Sbjct: 47  RKVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGT 106

Query: 73  IFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVFNHIFAR 132
           ++P+NGGYV+W+S+A GPFWGFQ+G+ KW SG +DNALYPVLFLDYLK   P       R
Sbjct: 107 MYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPR 166

Query: 133 IPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRISPKKWLILEYSK 192
           + ++L +T  LTYLNYRGL IVG  AV++ VFS+ PF VM L+SIP++ P +WL+++   
Sbjct: 167 VASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGN 226

Query: 193 VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSSYLIPLLAGTGAL 252
           VNW  Y N++FWNLNYWD  STLAGEVENP+ T P+A+F  V+LV  SY+ PLLAG GA+
Sbjct: 227 VNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAI 286

Query: 253 ATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMI 312
             +  +W+DGYF++V   +GG WL+WW+QAAAA SNMG+F AEMSSD++QLLGM+E GM+
Sbjct: 287 PLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGML 346

Query: 313 PSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRIR 372
           P  FA RS+YGTP   IL SA GV+ LSW+SFQEI+   N LY VGM+LEF AF+++R++
Sbjct: 347 PEFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMK 406

Query: 373 KPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIAVGFLLYPTLLQA 432
            P  +RPYK+P+ T G  L+C PP+ L+  V+ L+S K   +S +++ +GFL++P L   
Sbjct: 407 HPAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHM 466

Query: 433 KNRRWVKF 441
             +RWVKF
Sbjct: 467 DRKRWVKF 473

[Close]

BLAST of MELO3C018511 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 535.0 bits (1377), Expect = 6.1e-152
Identity = 249/428 (58.18%), Postives = 330/428 (77.10%), Query Frame = 0


Query: 13  KKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWSIPEALVTAELAT 72
           +K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGF++FPFIWSIPEAL+TAE+ T
Sbjct: 31  RKVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGT 90

Query: 73  IFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVFNHIFAR 132
           ++P+NGGYV+W+S+A GPFWGFQ+G+ KW SG +DNALYPVLFLDYLK   P       R
Sbjct: 91  MYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPR 150

Query: 133 IPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRISPKKWLILEYSK 192
           + ++L +T  LTYLNYRGL IVG  AV++ VFS+ PF VM L+SIP++ P +WL+++   
Sbjct: 151 VASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGN 210

Query: 193 VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSSYLIPLLAGTGAL 252
           VNW  Y N++FWNLNYWD  STLAGEVENP+ T P+A+F  V+LV  SY+ PLLAG GA+
Sbjct: 211 VNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAI 270

Query: 253 ATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMI 312
             +  +W+DGYF++V   +GG WL+WW+QAAAA SNMG+F AEMSSD++QLLGM+E GM+
Sbjct: 271 PLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGML 330

Query: 313 PSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRIR 372
           P  FA RS+YGTP   IL SA GV+ LSW+SFQEI+   N LY VGM+LEF AF+++R++
Sbjct: 331 PEFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMK 390

Query: 373 KPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIAVGFLLYPTLLQA 432
            P  +RPYK+P+ T G  L+C PP+ L+  V+ L+S K   +S +++ +GFL++P L   
Sbjct: 391 HPAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHM 450

Query: 433 KNRRWVKF 441
             +RWVKF
Sbjct: 451 DRKRWVKF 457

[Close]

BLAST of MELO3C018511 vs. TAIR 10
Match: AT1G31820.1 (Amino acid permease family protein )

HSP 1 Score: 498.4 bits (1282), Expect = 6.4e-141
Identity = 241/428 (56.31%), Postives = 319/428 (74.53%), Query Frame = 0


Query: 13  KKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWSIPEALVTAELAT 72
           +K+++LPL+ LIFY+VSGGPFG E SV+   GPLLALLGF++FPFIW IPEAL+TAE++T
Sbjct: 34  QKVSMLPLVFLIFYEVSGGPFGAEGSVN-AAGPLLALLGFVIFPFIWCIPEALITAEMST 93

Query: 73  IFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLKRSFPVFNHIFAR 132
           +FP NGG+V+W+S+A G FWGFQ G+ KW  G +DNALYPVLFLDYLK + P       R
Sbjct: 94  MFPINGGFVVWVSSALGTFWGFQVGWMKWLCGVIDNALYPVLFLDYLKSAVPALATGLPR 153

Query: 133 IPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRISPKKWLILEYSK 192
           + ++L +T  LTYLNYRGL IVG +AV + VFS+ PF VM+L+SIP++ P +WL+++   
Sbjct: 154 VASILILTLLLTYLNYRGLTIVGWTAVFMGVFSMLPFAVMSLVSIPQLEPSRWLVMDLGN 213

Query: 193 VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSSYLIPLLAGTGAL 252
           VNW  Y N++ WNLNYWD  STLAGEV NP KT P+A+   V+ V  S  +PLL+GTGA+
Sbjct: 214 VNWNLYLNTLLWNLNYWDSVSTLAGEVANPKKTLPKALCYGVIFVALSNFLPLLSGTGAI 273

Query: 253 ATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMI 312
             D   W+DGY AEV   IGG WL+ W+QAAAA SNMG+F AEMSSD++QLLGM+E+G++
Sbjct: 274 PLDRELWTDGYLAEVAKAIGGGWLQLWVQAAAATSNMGMFLAEMSSDSFQLLGMAELGIL 333

Query: 313 PSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGMLLEFAAFIKLRIR 372
           P +FA RS+YGTP   IL SA GV+ LS +SFQEI+   N LY  GM+LEF AF++LR +
Sbjct: 334 PEIFAQRSRYGTPLLGILFSASGVLLLSGLSFQEIIAAENLLYCGGMILEFIAFVRLRKK 393

Query: 373 KPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIAVGFLLYPTLLQA 432
            P  +RPYK+P+ TVG  L+C PP  L+ LV+ L++ K  L+S +++ +GFL+ P L   
Sbjct: 394 HPAASRPYKIPVGTVGSILICVPPIVLICLVIVLSTIKVALVSFVMVVIGFLMKPCLNHM 453

Query: 433 KNRRWVKF 441
             ++WVKF
Sbjct: 454 DGKKWVKF 460

The following BLAST results are available for this feature:

BLAST of MELO3C018511 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008455023.1	3.9e-273	100.00	PREDICTED: probable polyamine transporter At3g19553 isoform X1 [Cucumis melo]	[more]
KAA0031359.1	8.6e-273	99.79	putative polyamine transporter [Cucumis melo var. makuwa] >TYK06811.1 putative p...	[more]
XP_004136943.1	3.0e-265	96.50	probable polyamine transporter At3g19553 [Cucumis sativus] >KGN43858.1 hypotheti...	[more]
XP_038888395.1	1.5e-256	93.22	probable polyamine transporter At3g19553 [Benincasa hispida]	[more]
XP_022972448.1	7.1e-243	89.32	probable polyamine transporter At3g19553 [Cucurbita maxima]	[more]

BLAST of MELO3C018511 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LH39	9.7e-203	75.22	Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g...	[more]
Q9FFL1	5.1e-151	59.07	Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1	[more]
Q9C6S5	8.6e-151	58.18	Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g...	[more]
A2X8M8	1.1e-145	58.04	Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S...	[more]
Q6Z8D0	1.1e-145	58.04	Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1...	[more]

BLAST of MELO3C018511 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C014	1.9e-273	100.00	probable polyamine transporter At3g19553 isoform X1 OS=Cucumis melo OX=3656 GN=L...	[more]
A0A5D3C4H2	4.2e-273	99.79	Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A0A0K2Y2	1.4e-265	96.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071470 PE=3 SV=1	[more]
A0A6J1I9Z5	3.4e-243	89.32	probable polyamine transporter At3g19553 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1GKM4	5.0e-242	88.71	probable polyamine transporter At3g19553 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of MELO3C018511 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19553.1	6.9e-204	75.22	Amino acid permease family protein	[more]
AT5G05630.1	3.6e-152	59.07	Amino acid permease family protein	[more]
AT1G31830.1	6.1e-152	58.18	Amino acid permease family protein	[more]
AT1G31830.2	6.1e-152	58.18	Amino acid permease family protein	[more]
AT1G31820.1	6.4e-141	56.31	Amino acid permease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002293	Amino acid/polyamine transporter I	PFAM	PF13520	AA_permease_2	coord: 20..421 e-value: 8.6E-31 score: 107.3
None	No IPR available	PIRSF	PIRSF006060	AA_transporter	coord: 4..456 e-value: 1.9E-69 score: 231.9
None	No IPR available	GENE3D	1.20.1740.10	Amino acid/polyamine transporter I	coord: 11..444 e-value: 1.1E-55 score: 191.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 459..486
None	No IPR available	PANTHER	PTHR45826:SF21	BNAA05G20530D PROTEIN	coord: 3..451
IPR044566	Polyamine transporter RMV1-like	PANTHER	PTHR45826	POLYAMINE TRANSPORTER PUT1	coord: 3..451

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C018511.1	MELO3C018511.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity