MELO3C018027 (gene) Melon (DHL92) v4

Overview
NameMELO3C018027
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionPyruvate kinase
Locationchr07: 26343709 .. 26348662 (-)
RNA-Seq ExpressionMELO3C018027
SyntenyMELO3C018027
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCCAACTTTATTTTCTAAAATGTTTATGTTTTAAAAATGAAGTCTACAAACGCTCTTTACATCTTTGAATTTCTTATTTTTTATCCTTATTCTTTATCAATGTTTTCGAAGTTCAAGTTTAAATTTTGAAAATAAAAAAATAGTTTTTTTTGAAAAAATTGTCAAATGACACCTAATGGGGTGTTTGGAGTTGATAACCACTTTTTTTTATTACCGTAAATGCTATTTTATACTCACTGTTTAGCTACGGTCAACGTTATTTGAGACCTTTCATTTACTAGGGGTAATTATCGTGTTCACAAAGAGTGACATTTTGTTGATTTGATTTAGATTTCATTTGAAAGACGTTAGAAGTAAATAAATGATAATTCAAGAGAAGTATTTGTGAAAGATTTTCGAAAATTCCAAAAGAATCGGGAGGGAGGGGGGAAGAGAAAAGGGAAAAAGCGGTTTTGGGTAAGACAGAACTGCAGGAAACAGTGGGTGCCACGTGAGGTTTAGAGGTTGAACCTAAACCACAAGCCAACGCCGGGAAAGTTAGTTGACCGGTAAAGTTCATATTAATTTATCACTTTTTAACACCTAAACACACTATGACAAAACTCTTCTTCCCATTCACAAATAATTAGGGTTCTTCTAATCGAATATTCTTCTTCTTCTTTCTTGTTAATTCCATCTTTGTTCACTTCCTTACTCCTTTTTCCAAACTCAGCGGACTATACTATTTGAACCTATCTAAGGCCTCTTCTTGCAGAAGAAGGCCGAAGATATATAGTTGCTGAGCTTTTTATTCTTCTTTCTTCTTTTTCGTTCATGGCGGAGGTCAATGCTATGATGATTCGTGCTCGCTGTAATTTTATGGTGAGAAACCCTGTGTTGGATGTGAAGCGTTTGGGTTTCCGGATGCCGGTTATGGGTCACCGAACAGTTTGGTGTAATCGGAGGAGGATGAAGGGGAGAGTTGGAAATGGAGTGAAGGCGACAATGCAGGTTGATTTGGACGATGAGGAGAGGTTGAGAAGATTGGAGGGTTTAGAGGCAACGTTAGGGCTTGATATTGTAGCGGAGAGGGAACTGAAGGAGAAAGGGTTTTTAGGGATGAGGAAGACGAAGCTTGTATGTACTATTGGTCCAGCGTGTAGTGGGATTGAGGATTTGGAGAAGTTAGCGTTGGAAGGTATGAATATTGCGAGATTGAATATGTGTCACAACTCGAGGGAGTGGCATTGTGATATGATTAAGAAAATCAAGAAGTTAAATGAGGAGAAAGGATTCTGTATTTCGGTTATGATTGATATTGAAGGAAGTCGGATTCATGTGGTTGATCACGGAGCTCCTTCCTCTGTAAAAGTTGAGGTAAATCGTAATTGTCATGTCTCTGTTTTCGTTGTTTCCCTTTACTGTAGTCTAAATTCTCTGGTCATCATTGTTCAGGATGGTTCGATCTGGTTGTTTACTGCTGAGAAGTTCGAAGGTTCTCGTCCATTCACTGTGCAAGCGAGCTATGAAGGTTTCTCTGAAGGTGCGTCTTGAATCGTGTATTCTATTTGTGATTAGAAAAAGATATTTGAATCCGAGAAGCTAACTAGGACTTGATTTTGAAGTTGATGATCTTGTTTTTACGAACTTGGGCAGGTATCCGAGTGGGTGATTCAATTGTAATTGATGGGGGAATGGCAACCTTTGAAGTTACTGAAAAGATTGGGAATGATTTAAGCTGCAGGTGCACAGACCCAGGTTTATTTCTTCCCCGGGCGAAGTGTAGCTTTTGGAGAGATGGGAGGCTTGTTGTGAGAAATCACGAGTCTCCCACGTTGTCAAGTAAGGTAGGAACGTTTTTCCTGCAATATCAATATTCATACTGAGAAATTAATTTGTGATAAATATAACTAGAAGTGCTCACTTTTAAGTTAAACTTTCAAGTATGATGTATTCAAAGATCAAGAACCTAAGATGTTGCACTATTCGTTTTGGGCATTACTATGGAGGAAATCTGATGTCCATTATTTTTTAAGTTTTCTTTCTGTGGGATTACAAGTTGGGTCCACTATCTTGTGGAGTCGCGAACAGGTTCATCGTTTGAAATTTTTTCTTACTCTGGGTTGTCAACATGGCAATGTTTTTACTTGATTCAATATGGGAACAACAGTTTAAGGTTCATTATTGAAGAGTTGGCTTCTTCTTGTCGTGGTTGTAAACACTACGAGTTTTCGCTCCCGAATGATATTGTGGATGAATTTTAAGGATCAATTTCAATTTTTTTCTTGTTTGGGTGTCAGCATCAAAAGGTTTTGTTCCTGGACATTTTCATCTCTGTCGTGAAGAAAACTTATCTATTTTTCTGCACGTAAAACTAATTTTCTTGACAATTGTTTCTCCTTTGACGAGGTTGACTTTCTCATGTTAATGCAGAGTAAAAGGTCTTGGTATTATGTAATTAAATTTATCTAATCTATCAACTTATATGTTTTTAGGTTGATCGATGTATTGGATCTTTCTGATTAATGTCGTTTCTTCTAATTAATATTAATTTTCATGTGTGAACTTTTTACAAATTTATAATTCACGAGTAAGGGAGTATTAAACTATTGAAATAATTTCCGGGTTGATATGGAATTCTATAAATCAAGTCTTTTTGGATGATGCGGTTCTCTGCATTTAAACCCTGTTTTTTCCATGGTGTATTACGTTATTCGGTTTTCCGTTTTACAGGATTGGTCGGACATTGAATTTGGAATTTCAGAGGGCGTGGACTTCATTGCCCTTTCTTTTGTGAACAGTGCAGATCCTGTCAGACATCTAAAAAACTACCTTTTAACAAAATCAGCAAAGTAAGCATCAATTCAAGAAGCCAACATAACCATGAGTTAGGTTGAAATATCACTATCACTACTATGCATGAACTACTGATTCTTCTCCTTTTTTAATCGAATTTTCAACAGCATTTCATAAGATGGATTCCCATAGGCCGACACTATTGAGGCATTATTTTCCGCAAATTTTGATTACTGAAACAGATTGGGTAATTTTAAAACTTCGTTCAAGTGATGAAGGGTACATTCTCTTTTTCATGCACAGATCCATAAGGGTATTGGCTAAGATTGAAAGCTTGGAGGCCCTTCAAAATCTGGAAGAAATTATAGAGGCCTCTGATGGAATCATGGTGGCTCGAGGGGACCTTGGGGTCGAAATTCCGCTCGAGCAGATACCGGCAGTGCAAGAGGAAATAACCAGAGTCTGTAGGGAACTGAACAAACCAGTGATCATAGCTTCTCAACTTCTTGAGTCCATGGTTGAATATCCTACCCCAACACGTGCTGAGGTACAAGTGACATGTGCTTTTGATTATTTCCAGTTTTGGACTTGTTTGAAATGACTATCTAAGTATTTAAAAAAGTGTCTTTTCAAGGCTCTTCTTGTGCTCTCCAGAAGGCTGATATTTCATACTGTTCGTGTTCAATAGGTTGCAGATGTTTCTGAAGCTGTCCGACAATATGCTGATGCATTGATGCTATCTGGGGAGTCTGCCATAGGATCATATGGACAGAAAGCACTTTCGGTTCTACAGATGGCCAGCAGCCGAATGGAATTATGGAGTCGTGAAGAAAATACGAAAAATTTCCTTCCCCAGCATCAACTTGGAGTATCATTGCATGATCGAATCGCAGAAGAAATTTGCAATTCTGCAGCTGAATTGGGTAAAACTCGCTTTTCTCCACTTTCTTTTTAAACAAAGTCCATCGTATGGGATCGTCAAAATAACGTCATAATTCCCTTTTGGCTATGTCTCATGTCTATCTGCTACCTTCTTTGTTACGTAATCACGCAAAAGAAAATCGTATGAAATATATTGGCTTGCCATTTTAGTCCATTCTTCTGCGCTTAGTTTCAATGTGGTATTTCTGGTTTAGAAGTATATATCATCAAAATCCTTCAAAAACATATCGGCTGGATGTTTTCACTCATTTCTGTTCTGAGTCACTGTCACACTGTTCTAGGTCAAAATTTTAAAGATCGTTTACTTATAAAGTAATTTTAAGAACCTTTATAAAAGGAAGAGTAGAGTAATATCGTAACTTTCACAAGTACAGAGCTAGTTTTGAAACAAATGGTCAGTTAGAGGATATTTTCTTATGTTAACCTTAAAAGTTGTTAACAATTGGTAACAACAGAGAAGTTTTGTGAGAAAAAGCAGGTTGTGTCATGGTGTGGTAGAACTAGAACCGAGTGTTTGATTCTTAGGTTTGAAGAAGTAAATCAATTACCTTCAAAACAAGAGCTTCTGTTTGTTTGAATGTTGCTAATTGCATTTGGCCCACGTTTCCACTAAAATAAAAACCTCCATATTCTTTGTGGCAGCTAACAGACTTTCAGTAGACGCCATATTTGTGCTCACAAATCAAGGGCATATGGCATCTCTCCTTTCCCGAAACCGCCCAGGGCCGCCAATATTTGCATTAACGGACGATGATAGCACAAGAATGGCTTTGAATCTACAATGGGGAGTGTTCCCTTTGCGAATTGACCTCTCAGAAGATATTGAAGCAAACATCTCGAGAGGGATCGAGGTTGTGAAATCAAAGGGATTGGTGAAACAGGGGGACTCTGTGCTTGTGGTCTCAGAGATCTCTCCAGCTCGAGCTGTATCAATGGCGTCCCAATCAATTCAGCTGAAAACCATAGTGTAGACCAAAGAGAAATCAGGTCAAGCTTTGTAAGTGAAACTTTACGATTGCATGTATCATATGTGGTGGATATTACACATGAATGTTGCATCTTATCATTTGTGGCCCTCTGTTTGTAACACTATTGAAGCTTGGATAGGTTGTTTATTGTTTTGTACGAGCTAATATTTGGAAAATTCAATAACTCTACTCAAATTTGAATTTAGTTTTATGGTTTGTTTTTATTTGTTTATATATTTATTTAAACTAAAAATACTCTCTTTTTTA

mRNA sequence

TTTCCAACTTTATTTTCTAAAATGTTTATGTTTTAAAAATGAAGTCTACAAACGCTCTTTACATCTTTGAATTTCTTATTTTTTATCCTTATTCTTTATCAATGTTTTCGAAGTTCAAGTTTAAATTTTGAAAATAAAAAAATAGTTTTTTTTGAAAAAATTGTCAAATGACACCTAATGGGGTGTTTGGAGTTGATAACCACTTTTTTTTATTACCGTAAATGCTATTTTATACTCACTGTTTAGCTACGGTCAACGTTATTTGAGACCTTTCATTTACTAGGGGTAATTATCGTGTTCACAAAGAGTGACATTTTGTTGATTTGATTTAGATTTCATTTGAAAGACGTTAGAAGTAAATAAATGATAATTCAAGAGAAGTATTTGTGAAAGATTTTCGAAAATTCCAAAAGAATCGGGAGGGAGGGGGGAAGAGAAAAGGGAAAAAGCGGTTTTGGGTAAGACAGAACTGCAGGAAACAGTGGGTGCCACGTGAGGTTTAGAGGTTGAACCTAAACCACAAGCCAACGCCGGGAAAGTTAGTTGACCGGTAAAGTTCATATTAATTTATCACTTTTTAACACCTAAACACACTATGACAAAACTCTTCTTCCCATTCACAAATAATTAGGGTTCTTCTAATCGAATATTCTTCTTCTTCTTTCTTGTTAATTCCATCTTTGTTCACTTCCTTACTCCTTTTTCCAAACTCAGCGGACTATACTATTTGAACCTATCTAAGGCCTCTTCTTGCAGAAGAAGGCCGAAGATATATAGTTGCTGAGCTTTTTATTCTTCTTTCTTCTTTTTCGTTCATGGCGGAGGTCAATGCTATGATGATTCGTGCTCGCTGTAATTTTATGGTGAGAAACCCTGTGTTGGATGTGAAGCGTTTGGGTTTCCGGATGCCGGTTATGGGTCACCGAACAGTTTGGTGTAATCGGAGGAGGATGAAGGGGAGAGTTGGAAATGGAGTGAAGGCGACAATGCAGGTTGATTTGGACGATGAGGAGAGGTTGAGAAGATTGGAGGGTTTAGAGGCAACGTTAGGGCTTGATATTGTAGCGGAGAGGGAACTGAAGGAGAAAGGGTTTTTAGGGATGAGGAAGACGAAGCTTGTATGTACTATTGGTCCAGCGTGTAGTGGGATTGAGGATTTGGAGAAGTTAGCGTTGGAAGGTATGAATATTGCGAGATTGAATATGTGTCACAACTCGAGGGAGTGGCATTGTGATATGATTAAGAAAATCAAGAAGTTAAATGAGGAGAAAGGATTCTGTATTTCGGTTATGATTGATATTGAAGGAAGTCGGATTCATGTGGTTGATCACGGAGCTCCTTCCTCTGTAAAAGTTGAGGATGGTTCGATCTGGTTGTTTACTGCTGAGAAGTTCGAAGGTTCTCGTCCATTCACTGTGCAAGCGAGCTATGAAGGTTTCTCTGAAGGTATCCGAGTGGGTGATTCAATTGTAATTGATGGGGGAATGGCAACCTTTGAAGTTACTGAAAAGATTGGGAATGATTTAAGCTGCAGGTGCACAGACCCAGGTTTATTTCTTCCCCGGGCGAAGTGTAGCTTTTGGAGAGATGGGAGGCTTGTTGTGAGAAATCACGAGTCTCCCACGTTGTCAAGTAAGGATTGGTCGGACATTGAATTTGGAATTTCAGAGGGCGTGGACTTCATTGCCCTTTCTTTTGTGAACAGTGCAGATCCTGTCAGACATCTAAAAAACTACCTTTTAACAAAATCAGCAAAATCCATAAGGGTATTGGCTAAGATTGAAAGCTTGGAGGCCCTTCAAAATCTGGAAGAAATTATAGAGGCCTCTGATGGAATCATGGTGGCTCGAGGGGACCTTGGGGTCGAAATTCCGCTCGAGCAGATACCGGCAGTGCAAGAGGAAATAACCAGAGTCTGTAGGGAACTGAACAAACCAGTGATCATAGCTTCTCAACTTCTTGAGTCCATGGTTGAATATCCTACCCCAACACGTGCTGAGGTTGCAGATGTTTCTGAAGCTGTCCGACAATATGCTGATGCATTGATGCTATCTGGGGAGTCTGCCATAGGATCATATGGACAGAAAGCACTTTCGGTTCTACAGATGGCCAGCAGCCGAATGGAATTATGGAGTCGTGAAGAAAATACGAAAAATTTCCTTCCCCAGCATCAACTTGGAGTATCATTGCATGATCGAATCGCAGAAGAAATTTGCAATTCTGCAGCTGAATTGGCTAACAGACTTTCAGTAGACGCCATATTTGTGCTCACAAATCAAGGGCATATGGCATCTCTCCTTTCCCGAAACCGCCCAGGGCCGCCAATATTTGCATTAACGGACGATGATAGCACAAGAATGGCTTTGAATCTACAATGGGGAGTGTTCCCTTTGCGAATTGACCTCTCAGAAGATATTGAAGCAAACATCTCGAGAGGGATCGAGGTTGTGAAATCAAAGGGATTGGTGAAACAGGGGGACTCTGTGCTTGTGGTCTCAGAGATCTCTCCAGCTCGAGCTGTATCAATGGCGTCCCAATCAATTCAGCTGAAAACCATAGTGTAGACCAAAGAGAAATCAGGTCAAGCTTTGTAAGTGAAACTTTACGATTGCATGTATCATATGTGGTGGATATTACACATGAATGTTGCATCTTATCATTTGTGGCCCTCTGTTTGTAACACTATTGAAGCTTGGATAGGTTGTTTATTGTTTTGTACGAGCTAATATTTGGAAAATTCAATAACTCTACTCAAATTTGAATTTAGTTTTATGGTTTGTTTTTATTTGTTTATATATTTATTTAAACTAAAAATACTCTCTTTTTTA

Coding sequence (CDS)

ATGGCGGAGGTCAATGCTATGATGATTCGTGCTCGCTGTAATTTTATGGTGAGAAACCCTGTGTTGGATGTGAAGCGTTTGGGTTTCCGGATGCCGGTTATGGGTCACCGAACAGTTTGGTGTAATCGGAGGAGGATGAAGGGGAGAGTTGGAAATGGAGTGAAGGCGACAATGCAGGTTGATTTGGACGATGAGGAGAGGTTGAGAAGATTGGAGGGTTTAGAGGCAACGTTAGGGCTTGATATTGTAGCGGAGAGGGAACTGAAGGAGAAAGGGTTTTTAGGGATGAGGAAGACGAAGCTTGTATGTACTATTGGTCCAGCGTGTAGTGGGATTGAGGATTTGGAGAAGTTAGCGTTGGAAGGTATGAATATTGCGAGATTGAATATGTGTCACAACTCGAGGGAGTGGCATTGTGATATGATTAAGAAAATCAAGAAGTTAAATGAGGAGAAAGGATTCTGTATTTCGGTTATGATTGATATTGAAGGAAGTCGGATTCATGTGGTTGATCACGGAGCTCCTTCCTCTGTAAAAGTTGAGGATGGTTCGATCTGGTTGTTTACTGCTGAGAAGTTCGAAGGTTCTCGTCCATTCACTGTGCAAGCGAGCTATGAAGGTTTCTCTGAAGGTATCCGAGTGGGTGATTCAATTGTAATTGATGGGGGAATGGCAACCTTTGAAGTTACTGAAAAGATTGGGAATGATTTAAGCTGCAGGTGCACAGACCCAGGTTTATTTCTTCCCCGGGCGAAGTGTAGCTTTTGGAGAGATGGGAGGCTTGTTGTGAGAAATCACGAGTCTCCCACGTTGTCAAGTAAGGATTGGTCGGACATTGAATTTGGAATTTCAGAGGGCGTGGACTTCATTGCCCTTTCTTTTGTGAACAGTGCAGATCCTGTCAGACATCTAAAAAACTACCTTTTAACAAAATCAGCAAAATCCATAAGGGTATTGGCTAAGATTGAAAGCTTGGAGGCCCTTCAAAATCTGGAAGAAATTATAGAGGCCTCTGATGGAATCATGGTGGCTCGAGGGGACCTTGGGGTCGAAATTCCGCTCGAGCAGATACCGGCAGTGCAAGAGGAAATAACCAGAGTCTGTAGGGAACTGAACAAACCAGTGATCATAGCTTCTCAACTTCTTGAGTCCATGGTTGAATATCCTACCCCAACACGTGCTGAGGTTGCAGATGTTTCTGAAGCTGTCCGACAATATGCTGATGCATTGATGCTATCTGGGGAGTCTGCCATAGGATCATATGGACAGAAAGCACTTTCGGTTCTACAGATGGCCAGCAGCCGAATGGAATTATGGAGTCGTGAAGAAAATACGAAAAATTTCCTTCCCCAGCATCAACTTGGAGTATCATTGCATGATCGAATCGCAGAAGAAATTTGCAATTCTGCAGCTGAATTGGCTAACAGACTTTCAGTAGACGCCATATTTGTGCTCACAAATCAAGGGCATATGGCATCTCTCCTTTCCCGAAACCGCCCAGGGCCGCCAATATTTGCATTAACGGACGATGATAGCACAAGAATGGCTTTGAATCTACAATGGGGAGTGTTCCCTTTGCGAATTGACCTCTCAGAAGATATTGAAGCAAACATCTCGAGAGGGATCGAGGTTGTGAAATCAAAGGGATTGGTGAAACAGGGGGACTCTGTGCTTGTGGTCTCAGAGATCTCTCCAGCTCGAGCTGTATCAATGGCGTCCCAATCAATTCAGCTGAAAACCATAGTGTAG

Protein sequence

MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQVDLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLALEGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCRCTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPVRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISRGIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV
Homology
BLAST of MELO3C018027 vs. NCBI nr
Match: XP_008454424.1 (PREDICTED: plastidial pyruvate kinase 1, chloroplastic-like [Cucumis melo] >ADN33819.1 pyruvate kinase [Cucumis melo subsp. melo] >TYK29442.1 plastidial pyruvate kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 582/582 (100.00%), Postives = 582/582 (100.00%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120
           DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 583
           GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of MELO3C018027 vs. NCBI nr
Match: KAA0044313.1 (plastidial pyruvate kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 581/582 (99.83%), Postives = 581/582 (99.83%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120
           DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 583
           GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of MELO3C018027 vs. NCBI nr
Match: XP_004150267.1 (plastidial pyruvate kinase 1, chloroplastic [Cucumis sativus] >KGN52735.1 hypothetical protein Csa_015140 [Cucumis sativus])

HSP 1 Score: 1110.1 bits (2870), Expect = 0.0e+00
Identity = 565/582 (97.08%), Postives = 573/582 (98.45%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMG RTVWC+RRRMKGRV NGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVVNGVKATMQV 60

Query: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120
           DL+DEERL+RLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCT+GPACSGI DLEKLAL
Sbjct: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS EWHCDMIKKIKKLNEEKGFCISVMIDIEGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240
           EDGSIWLFTA+KFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLV+RNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYL TKSAKSIRVLAKIESLEALQNLEEI+EASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN  NFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 583
           GIEVVKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582

BLAST of MELO3C018027 vs. NCBI nr
Match: XP_038905899.1 (plastidial pyruvate kinase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1061.6 bits (2744), Expect = 2.4e-306
Identity = 541/582 (92.96%), Postives = 564/582 (96.91%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60
           MAEVNA+MIR+R +F+ ++PVLDVKR GF MPV+G RTVWCNRRR +G VGNGVKATMQV
Sbjct: 1   MAEVNAIMIRSRFDFIAKSPVLDVKRSGFGMPVIG-RTVWCNRRRTRGGVGNGVKATMQV 60

Query: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120
           DL+DEERL+RLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPAC+ IEDLEKLAL
Sbjct: 61  DLEDEERLKRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACNAIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNSREWHCD+I+KIKKLNE+KGFCISVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEKKGFCISVMIDTEGSQIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGI+VGDSIVIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDI+FGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIKFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYLLTKSAKS+RVLAKIESLE+LQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VQHLKNYLLTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRME WSREEN KNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMESWSREENRKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRP P IFALTDDDSTRMALNLQWGV PLRID SEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPSPLIFALTDDDSTRMALNLQWGVVPLRIDFSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 583
           GIE+VKSKGLVK+GDSVLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIELVKSKGLVKKGDSVLVVSEISPARAASMASQSIQLKTIV 581

BLAST of MELO3C018027 vs. NCBI nr
Match: XP_023553283.1 (plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1055.0 bits (2727), Expect = 2.3e-304
Identity = 537/581 (92.43%), Postives = 557/581 (95.87%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRA CNF+ +NPVLDVKR GF MPV G RTVWCNRRR  GRVGNGVKA MQV
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFG-RTVWCNRRRTTGRVGNGVKAVMQV 60

Query: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120
           DL+D ERLRRLEGLEATLGLD+VAERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNSREWHCD+I+KIKKLNE KGFCISVMID EGS+IHVVDHGAPSS+KV
Sbjct: 121 EGMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGI+VGDS+VIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGR+VVRNHESPTLSSKDWSDIEFGI+EGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYL TKSA+S+RVLAKIESLE+LQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VKHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEIT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMAS RMELWSREEN +NFLP +QLGVSL DRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLT QGHMASLLSRNRP PPIFALTDDDSTRMALNLQWGVFPLRI+LSEDIEANI R
Sbjct: 481 AIFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTI 582
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTI
Sbjct: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 580

BLAST of MELO3C018027 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 1.0e-169
Identity = 293/487 (60.16%), Postives = 370/487 (75.98%), Query Frame = 0

Query: 80  LDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLALEGMNIARLNMCHNSREWHC 139
           +D V E ELKE GF   R+TKL+CTIGPA  G E LE LA+ GMN+ARLNMCH +R+WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 140 DMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRP- 199
            +I+ +++LNEEKGF +++M+D EGS IH+ D G  +S K EDG +W FT   F+ SRP 
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 200 FTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCRCTDPGLFLPRAKCSFWRD 259
            T+  SY+GF+E +RVGD +++DGGM  FEV EKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 260 GRLV-VRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPVRHLKNYLLTKS-AKSI 319
           G LV  RN   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ + HLK+YL  +S    I
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 320 RVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELNKPVI 379
            V+AKIES+++L NLEEII ASDG MVARGDLG +IPLEQ+PA Q+ I +VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 380 IASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRM 439
           +ASQLLESM+EYPTPTRAEVADVSEAVRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 440 ELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVLTNQGHMASLLS 499
           E W REE     +P   +G S  D+I+EEICNSAA++AN L VDA+FV T  GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 500 RNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISRGIEVVKSKGLVKQGDS 559
           R RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+++   ++KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 560 VLVVSEI 564
           V+ VS++
Sbjct: 580 VIAVSDM 586

BLAST of MELO3C018027 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.3e-169
Identity = 290/491 (59.06%), Postives = 377/491 (76.78%), Query Frame = 0

Query: 76  ATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLALEGMNIARLNMCHNSR 135
           +++ +D V E ELKE GF   R+TKLVCTIGPA  G E+LE LA+ GMN+AR+NMCH +R
Sbjct: 83  SSIEVDAVTETELKENGFRSTRRTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTR 142

Query: 136 EWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKVEDGSIWLFTAEKFEG 195
           EWH  +I+++++LNEEKGF +++M+D EGS IH+ D G  SS K EDG IW F+   ++ 
Sbjct: 143 EWHKSVIERVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDS 202

Query: 196 SRP-FTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCRCTDPGLFLPRAKCS 255
            RP  T+  +Y+GF+E ++VGD +++DGGM  FEV EKIG D+ CRCTDPGL LPRA  +
Sbjct: 203 PRPERTINVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLT 262

Query: 256 FWRDGRLV-VRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPVRHLKNYLLTKSA 315
           FWRDG LV  RN   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ + HLK+Y+  +S 
Sbjct: 263 FWRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSR 322

Query: 316 KS-IRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELN 375
            S I V+AKIES+++L+NLEEII ASDG MVARGDLG +IPLEQ+P+ Q+ I +VCR+LN
Sbjct: 323 DSDIAVIAKIESIDSLKNLEEIIRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLN 382

Query: 376 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMA 435
           KPVI+ASQLLESM+EYPTPTRAEVADVSEAVRQ ADALMLSGESA+G Y +KAL+VL+  
Sbjct: 383 KPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSV 442

Query: 436 SSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVLTNQGHMA 495
           S R+E W REE     +    +G +  D I+EEICNSAA++AN L VDA+FV T  GHMA
Sbjct: 443 SVRIEKWWREEKHHEAMELPAIGSTYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMA 502

Query: 496 SLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISRGIEVVKSKGLVK 555
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  ++D+E+N+++   ++K++G++K
Sbjct: 503 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIK 562

Query: 556 QGDSVLVVSEI 564
            GD V+ VS++
Sbjct: 563 SGDLVIAVSDM 573

BLAST of MELO3C018027 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 3.7e-164
Identity = 282/491 (57.43%), Postives = 374/491 (76.17%), Query Frame = 0

Query: 76  ATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLALEGMNIARLNMCHNSR 135
           +++ +D V E ELKE GF   R+TKL+CTIGPA  G E LE+LA  GMN+AR+NMCH +R
Sbjct: 93  SSIEVDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTR 152

Query: 136 EWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKVEDGSIWLFTAEKFEG 195
           EWH  +I+++++LNEEKGF +++M+D EGS IH+ D G  SS K EDG IW FT   F+ 
Sbjct: 153 EWHRMVIERLRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDP 212

Query: 196 SRP-FTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCRCTDPGLFLPRAKCS 255
             P  TV  +Y+GF+E ++VGD +++DGGM  FEV EKIG D+ C CTDPGL LPRA  +
Sbjct: 213 PLPERTVTVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLT 272

Query: 256 FWRDGRLV-VRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPVRHLKNYLLTKSA 315
           FWRDG+LV  RN   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ ++HLK+Y+  ++ 
Sbjct: 273 FWRDGKLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARAR 332

Query: 316 KS-IRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELN 375
            S I V+AKIES+++L+NLEEII+ASDG MVARGDLG +IPLEQ+P+ Q++I ++CR+LN
Sbjct: 333 DSDISVIAKIESIDSLKNLEEIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLN 392

Query: 376 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMA 435
           +PVI+ASQLLESM+EYP PTRAEVADVSEAVRQ  DALMLSGESA+G + +KAL+VL+  
Sbjct: 393 RPVIVASQLLESMIEYPIPTRAEVADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSV 452

Query: 436 SSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVLTNQGHMA 495
           S R+E   RE+     +    +  S  D I+EEICNSAA++AN L VDA+FV T  GHMA
Sbjct: 453 SLRIERMWREQKRHEVIELPSIASSFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMA 512

Query: 496 SLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISRGIEVVKSKGLVK 555
           SLLSR RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+++   ++K++G++K
Sbjct: 513 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIK 572

Query: 556 QGDSVLVVSEI 564
            GD ++ VS++
Sbjct: 573 SGDLIIAVSDM 583

BLAST of MELO3C018027 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 3.1e-86
Identity = 188/467 (40.26%), Postives = 278/467 (59.53%), Query Frame = 0

Query: 97  RKTKLVCTIGPACSGIEDLEKLALEGMNIARLNMCHNSREWHCDMIKKIKKLNEE-KGFC 156
           RKTK+VCTIGP+ S  E + KLA  GMN+ARLNM H     H   I  +K+ N + +   
Sbjct: 91  RKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDKV 150

Query: 157 ISVMIDIEGSRIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVG 216
           I++M+D +G    V+    P  + +++G  + F+ ++   +   TV  +Y+ F   +  G
Sbjct: 151 IAIMLDTKGP--EVISGDVPKPILLKEGQEFNFSIKRGVSTED-TVSVNYDDFINDVEAG 210

Query: 217 DSIVIDGGMATFEVTEKIGNDLSCRCTDPGLFLPRAKCSFWRDGRLVVRNHES--PTLSS 276
           D +++DGGM +  V  K  + + C   D G    R          L VR   +  P+++ 
Sbjct: 211 DILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSRR--------HLNVRGKSATLPSITE 270

Query: 277 KDWSDIEFGISEGVDFIALSFVNSADPVRHLKNYLLTKSAKSIRVLAKIESLEALQNLEE 336
           KDW DI+FG++  VDF A+SFV  A  V  LK+YL + +A  I V+ KIES +++ NL  
Sbjct: 271 KDWDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYLKSCNA-DIHVIVKIESADSIPNLHS 330

Query: 337 IIEASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELNKPVIIASQLLESMVEYPTPTR 396
           II ASDG MVARGDLG E+P+E++P +QE+I R C+ + KPVI+A+ +LESM+++PTPTR
Sbjct: 331 IISASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPTPTR 390

Query: 397 AEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRMELWSREENTKNFLPQHQ 456
           AEV+D+S AVR+ ADA+MLSGE+A G Y  KA+ V+ + + R E  S +++T +  P   
Sbjct: 391 AEVSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALRTES-SLQKSTSS--PSQS 450

Query: 457 LGVSLHDRIAEEICNSAAELANRLSVDAIFVLTNQGHMASLLSRNRPGPPIFALTDDDST 516
                H  + E     ++ +AN LS   I V T  G MA +LS NRP   +FA T+++  
Sbjct: 451 AAYKSH--MGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFTNNERV 510

Query: 517 RMALNLQWGVFPLRIDLSEDIEANISRGIEVVKSKGLVKQGDSVLVV 561
           +  L L  GV P+ ++ S D E   SR I+++ SK LVK G  V +V
Sbjct: 511 KQRLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLV 539

BLAST of MELO3C018027 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.2e-83
Identity = 203/591 (34.35%), Postives = 313/591 (52.96%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60
           M   N   +  R   +++     VK LG      G  +V  +RR +   V +    T  +
Sbjct: 14  MLSPNGGSVSTRSEKLLKPASFAVKVLGNEAKRSGRVSV-RSRRVVDTTVRSARVETEVI 73

Query: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGM-------RKTKLVCTIGPACSGIE 120
            +  E+   R E LE  L +    +        +GM       RKTK+VCT+GP+ +  E
Sbjct: 74  PVSPEDVPNREEQLERLLEMQQFGDTS------VGMWSKPTVRRKTKIVCTVGPSTNTRE 133

Query: 121 DLEKLALEGMNIARLNMCHNSREWHCDMIKKIKKLN-EEKGFCISVMIDIEGSRIHVVDH 180
            + KLA  GMN+AR+NM H     H  +I  +K+ N + K   I++M+D +G  +   D 
Sbjct: 134 MIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPEVRSGD- 193

Query: 181 GAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEK 240
             P  + ++ G  + FT E+   S P  V  +Y+ F   +  GD +++DGGM +F V  K
Sbjct: 194 -LPQPIMLDPGQEFTFTIER-GVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMVKSK 253

Query: 241 IGNDLSCRCTDPGLFLPRAKCSFWRDGRLVVRNHES--PTLSSKDWSDIEFGISEGVDFI 300
             + + C   D G    R          L VR   +  P+++ KDW DI+FG+   VDF 
Sbjct: 254 TKDSVKCEVVDGGELKSRR--------HLNVRGKSATLPSITEKDWEDIKFGVENKVDFY 313

Query: 301 ALSFVNSADPVRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGV 360
           A+SFV  A  V  LK Y L  S   I V+ KIES +++ NL  II ASDG MVARGDLG 
Sbjct: 314 AVSFVKDAQVVHELKKY-LQNSGADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGA 373

Query: 361 EIPLEQIPAVQEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADAL 420
           E+P+E++P +QEEI  +CR + K VI+A+ +LESM+ +PTPTRAEV+D++ AVR+ ADA+
Sbjct: 374 ELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAV 433

Query: 421 MLSGESAIGSYGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSA 480
           MLSGE+A G +  KA  V+   + R E       T   +P + LG +  + ++E     A
Sbjct: 434 MLSGETAHGKFPLKAAGVMHTVALRTE----ATITSGEMPPN-LGQAFKNHMSEMFAYHA 493

Query: 481 AELANRLSVDAIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDL 540
             ++N L    + V T  G MA LLS  RP   I+A T++   +  L L  GV P+ ++ 
Sbjct: 494 TMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEF 553

Query: 541 SEDIEANISRGIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTI 582
           ++D E   +  +  +  +G+VK+G+ + +V   +     S ++ +IQ++ +
Sbjct: 554 TDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

BLAST of MELO3C018027 vs. ExPASy TrEMBL
Match: A0A5D3E0J9 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00350 PE=3 SV=1)

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 582/582 (100.00%), Postives = 582/582 (100.00%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120
           DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 583
           GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of MELO3C018027 vs. ExPASy TrEMBL
Match: E5GBH7 (Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 582/582 (100.00%), Postives = 582/582 (100.00%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120
           DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 583
           GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of MELO3C018027 vs. ExPASy TrEMBL
Match: A0A1S3BYK3 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103494833 PE=3 SV=1)

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 582/582 (100.00%), Postives = 582/582 (100.00%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120
           DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 583
           GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of MELO3C018027 vs. ExPASy TrEMBL
Match: A0A5A7TLC2 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00350 PE=3 SV=1)

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 581/582 (99.83%), Postives = 581/582 (99.83%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120
           DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 583
           GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of MELO3C018027 vs. ExPASy TrEMBL
Match: A0A0A0KSY4 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000560 PE=3 SV=1)

HSP 1 Score: 1110.1 bits (2870), Expect = 0.0e+00
Identity = 565/582 (97.08%), Postives = 573/582 (98.45%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60
           MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMG RTVWC+RRRMKGRV NGVKATMQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVVNGVKATMQV 60

Query: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120
           DL+DEERL+RLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCT+GPACSGI DLEKLAL
Sbjct: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120

Query: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS EWHCDMIKKIKKLNEEKGFCISVMIDIEGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240
           EDGSIWLFTA+KFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCSFWRDGRLV+RNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           VRHLKNYL TKSAKSIRVLAKIESLEALQNLEEI+EASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN  NFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540
           AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 583
           GIEVVKSKGLVKQGDSVLVVSEISPARA SMASQSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582

BLAST of MELO3C018027 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 598.2 bits (1541), Expect = 7.1e-171
Identity = 293/487 (60.16%), Postives = 370/487 (75.98%), Query Frame = 0

Query: 80  LDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLALEGMNIARLNMCHNSREWHC 139
           +D V E ELKE GF   R+TKL+CTIGPA  G E LE LA+ GMN+ARLNMCH +R+WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 140 DMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRP- 199
            +I+ +++LNEEKGF +++M+D EGS IH+ D G  +S K EDG +W FT   F+ SRP 
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 200 FTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCRCTDPGLFLPRAKCSFWRD 259
            T+  SY+GF+E +RVGD +++DGGM  FEV EKIG D+ C CTDPGL LPRA  +FWRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 260 GRLV-VRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADPVRHLKNYLLTKS-AKSI 319
           G LV  RN   PT+SSKDW DI+FGI+EGVDFIA+SFV SA+ + HLK+YL  +S    I
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 320 RVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELNKPVI 379
            V+AKIES+++L NLEEII ASDG MVARGDLG +IPLEQ+PA Q+ I +VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 380 IASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRM 439
           +ASQLLESM+EYPTPTRAEVADVSEAVRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 440 ELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVLTNQGHMASLLS 499
           E W REE     +P   +G S  D+I+EEICNSAA++AN L VDA+FV T  GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 500 RNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISRGIEVVKSKGLVKQGDS 559
           R RP  PIFA T   S R  LNLQWG+ P R+  S+D+E+N+++   ++KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 560 VLVVSEI 564
           V+ VS++
Sbjct: 580 VIAVSDM 586

BLAST of MELO3C018027 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 310.8 bits (795), Expect = 2.3e-84
Identity = 203/591 (34.35%), Postives = 313/591 (52.96%), Query Frame = 0

Query: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60
           M   N   +  R   +++     VK LG      G  +V  +RR +   V +    T  +
Sbjct: 14  MLSPNGGSVSTRSEKLLKPASFAVKVLGNEAKRSGRVSV-RSRRVVDTTVRSARVETEVI 73

Query: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGM-------RKTKLVCTIGPACSGIE 120
            +  E+   R E LE  L +    +        +GM       RKTK+VCT+GP+ +  E
Sbjct: 74  PVSPEDVPNREEQLERLLEMQQFGDTS------VGMWSKPTVRRKTKIVCTVGPSTNTRE 133

Query: 121 DLEKLALEGMNIARLNMCHNSREWHCDMIKKIKKLN-EEKGFCISVMIDIEGSRIHVVDH 180
            + KLA  GMN+AR+NM H     H  +I  +K+ N + K   I++M+D +G  +   D 
Sbjct: 134 MIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNTIAIMLDTKGPEVRSGD- 193

Query: 181 GAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEK 240
             P  + ++ G  + FT E+   S P  V  +Y+ F   +  GD +++DGGM +F V  K
Sbjct: 194 -LPQPIMLDPGQEFTFTIER-GVSTPSCVSVNYDDFVNDVEAGDMLLVDGGMMSFMVKSK 253

Query: 241 IGNDLSCRCTDPGLFLPRAKCSFWRDGRLVVRNHES--PTLSSKDWSDIEFGISEGVDFI 300
             + + C   D G    R          L VR   +  P+++ KDW DI+FG+   VDF 
Sbjct: 254 TKDSVKCEVVDGGELKSRR--------HLNVRGKSATLPSITEKDWEDIKFGVENKVDFY 313

Query: 301 ALSFVNSADPVRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGV 360
           A+SFV  A  V  LK Y L  S   I V+ KIES +++ NL  II ASDG MVARGDLG 
Sbjct: 314 AVSFVKDAQVVHELKKY-LQNSGADIHVIVKIESADSIPNLHSIITASDGAMVARGDLGA 373

Query: 361 EIPLEQIPAVQEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADAL 420
           E+P+E++P +QEEI  +CR + K VI+A+ +LESM+ +PTPTRAEV+D++ AVR+ ADA+
Sbjct: 374 ELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVREGADAV 433

Query: 421 MLSGESAIGSYGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSA 480
           MLSGE+A G +  KA  V+   + R E       T   +P + LG +  + ++E     A
Sbjct: 434 MLSGETAHGKFPLKAAGVMHTVALRTE----ATITSGEMPPN-LGQAFKNHMSEMFAYHA 493

Query: 481 AELANRLSVDAIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDL 540
             ++N L    + V T  G MA LLS  RP   I+A T++   +  L L  GV P+ ++ 
Sbjct: 494 TMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKKIQQRLALYQGVCPIYMEF 553

Query: 541 SEDIEANISRGIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTI 582
           ++D E   +  +  +  +G+VK+G+ + +V   +     S ++ +IQ++ +
Sbjct: 554 TDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQSTHNIQVRKV 579

BLAST of MELO3C018027 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 307.8 bits (787), Expect = 1.9e-83
Identity = 191/492 (38.82%), Postives = 283/492 (57.52%), Query Frame = 0

Query: 97  RKTKLVCTIGPACSGIEDLEKLALEGMNIARLNMCHNSREWH---CDMIKKIKKLNEEKG 156
           RKTK+VCTIGP+ S  E + KLA  GMN+ARLNM H     H    D++K+   L  +K 
Sbjct: 99  RKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDK- 158

Query: 157 FCISVMIDIEGSRIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIR 216
             I++M+D +G  +   D   P  + +E+G  + FT ++    +  TV  +Y+ F   + 
Sbjct: 159 -AIAIMLDTKGPEVRSGD--VPQPIFLEEGQEFNFTIKRGVSLKD-TVSVNYDDFVNDVE 218

Query: 217 VGDSIVIDGGMATFEVTEKIGNDLSCRCTDPGLFLPRAKCSFWRDGRLVVRNHES--PTL 276
           VGD +++DGGM +  V  K  + + C   D G    R          L VR   +  P++
Sbjct: 219 VGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRR--------HLNVRGKSATLPSI 278

Query: 277 SSKDWSDIEFGISEGVDFIALSFVNSADPVRHLKNYLLTKSAKSIRVLAKIESLEALQNL 336
           + KDW DI+FG+   VDF A+SFV  A  V  LKNYL T SA  I V+ KIES ++++NL
Sbjct: 279 TDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKTCSA-DISVIVKIESADSIKNL 338

Query: 337 EEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELNKPVIIASQLLESMVEYPTP 396
             II A DG MVARGDLG E+P+E++P +QEEI R CR ++KPVI+A+ +LESM+ +PTP
Sbjct: 339 PSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTP 398

Query: 397 TRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRME--LWSREENTKNFL 456
           TRAEV+D++ AVR+ ADA+MLSGE+A G +  KA++V+   + R E  L  R   ++   
Sbjct: 399 TRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLPVRTSASRTTA 458

Query: 457 PQHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVLTNQGHMASLLSRNRPGPPIFALTD 516
            +  +G        +     A+ +AN LS   + V T  G MA LLS  RP   IFA T+
Sbjct: 459 YKGHMG--------QMFAFHASIMANTLS-SPLIVFTRTGSMAVLLSHYRPSATIFAFTN 518

Query: 517 DDSTRMALNLQWGVFPLRIDLSEDIEANISRGIEVVKSKGLVKQGDSVLVVSEISPARAV 576
                  L L  GV P+ ++ S+D E   +R +++++ + ++K+G  V +V   S     
Sbjct: 519 QRRIMQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIWR 567

Query: 577 SMASQSIQLKTI 582
             ++  IQ++ I
Sbjct: 579 EESTHLIQVRKI 567

BLAST of MELO3C018027 vs. TAIR 10
Match: AT3G04050.1 (Pyruvate kinase family protein )

HSP 1 Score: 228.4 bits (581), Expect = 1.5e-59
Identity = 162/515 (31.46%), Postives = 255/515 (49.51%), Query Frame = 0

Query: 98  KTKLVCTIGPACSGIEDLEKLALEGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCIS 157
           KTK+VCT+GPA   +E +EKL   GMN+AR N  H S  +H + +  ++   E      +
Sbjct: 17  KTKIVCTLGPASRSVEMVEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMENTCIPCA 76

Query: 158 VMIDIEGSRIHV--VDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVG 217
           VM+D +G  I    +  G P  + ++   I + T    EG    T+  SY+  +E ++ G
Sbjct: 77  VMLDTKGPEIRTGFLKEGKPVEL-IQGQEITISTDYTMEGDSN-TISMSYKKLAEDLKSG 136

Query: 218 DSIVIDGGMATFEVTEKIGNDLSCRCTDPGLFLPRAKCSFWRDGRLVVRNH--------E 277
           D I+   G  +  V       LSC   D  L L RA+C       L  R +        +
Sbjct: 137 DVILCSDGTISLTV-------LSC---DKNLGLVRARCE--NSAVLGERKNVNLPGIVVD 196

Query: 278 SPTLSSKDWSDI-EFGISEGVDFIALSFVNSADPVRHLKNYLLTKSAKSIRVLAKIESLE 337
            PTL+ KD  DI ++G+   +D IALSFV     +  ++  LL ++AKSI +++K+E+ E
Sbjct: 197 LPTLTEKDQEDILQWGVPNKIDIIALSFVRKGSDLVEVRK-LLGENAKSIMLMSKVENQE 256

Query: 338 ALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELNKPVIIASQLLESMV 397
            + N ++I+E SD  MVARGDLG+EIP+E++   Q+ + +    L KP++ A+Q+LESM 
Sbjct: 257 GVMNFDKILEYSDAFMVARGDLGMEIPIEKMFLAQKMMIQKANALGKPIVTATQMLESMT 316

Query: 398 EYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRMELWSREENTK 457
           + P PTRAE  DV+ AV    D +MLSGE+A G++ + A+  +       E         
Sbjct: 317 KSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPETAVLTMSRICKEAE--------- 376

Query: 458 NFLPQHQLGVSLHDRIA------EEICNSAAELANRLSVDAIFVLTNQGHMASLLSRNRP 517
           +F+    +   + D ++      E +  SA   A  L   AI VLT  G+   L+++ RP
Sbjct: 377 DFIDYDTMHKKIQDIVSLPLSPIESLAASAVSTARSLCAAAIVVLTKGGYTVELVAKYRP 436

Query: 518 GPPIFALTDDDSTRMALNLQW-----------------GVFPL-------RIDLSEDIEA 572
             PI ++   + TR   + +W                 G+ P+       R    +  E 
Sbjct: 437 SVPILSVIVPEITRTD-DFEWSCSESAAHVARRGLIYRGIVPVMATGASARSSNKDSTEE 496

BLAST of MELO3C018027 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 225.7 bits (574), Expect = 9.5e-59
Identity = 159/501 (31.74%), Postives = 253/501 (50.50%), Query Frame = 0

Query: 98  KTKLVCTIGPACSGIEDLEKLALEGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCIS 157
           KTK+VCT+GPA   +  +EKL   GMN+AR N  H S E+H + +  ++   +  G   +
Sbjct: 21  KTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRTAMQNTGILAA 80

Query: 158 VMIDIEGSRIHV--VDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVG 217
           VM+D +G  I    +  G P  +K E   I + T    +G    T+  SY+     ++ G
Sbjct: 81  VMLDTKGPEIRTGFLKDGNPIQLK-EGQEITITTDYDIKGDEK-TISMSYKKLPVDVKPG 140

Query: 218 DSIVIDGGMATFEVT--EKIGNDLSCRCTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSS 277
           ++I+   G  +  V   +     + CRC +  +   R   +    G +V    + PTL+ 
Sbjct: 141 NTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNL--PGVVV----DLPTLTD 200

Query: 278 KDWSDI-EFGISEGVDFIALSFVNSADPVRHLKNYLLTKSAKSIRVLAKIESLEALQNLE 337
           KD  DI ++G+   +D IALSFV     + +++  +L   +KSI +++K+E+ E + N +
Sbjct: 201 KDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRK-VLGSHSKSIMLMSKVENQEGVLNFD 260

Query: 338 EIIEASDGIMVARGDLGVEIPLEQIPAVQEEITRVCRELNKPVIIASQLLESMVEYPTPT 397
           EI+  +D  MVARGDLG+EIP+E+I   Q+ +   C    KPV+ A+Q+LESM++ P PT
Sbjct: 261 EILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQMLESMIKSPRPT 320

Query: 398 RAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRMELWSREENT--KNFLP 457
           RAE  DV+ AV    D +MLSGESA G+Y + A+  +       E  S + NT  K  + 
Sbjct: 321 RAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKTMAKICIEAES-SLDYNTIFKEMIR 380

Query: 458 QHQLGVSLHDRIAEEICNSAAELANRLSVDAIFVLTNQGHMASLLSRNRPGPPIFAL--- 517
              L +S      E + +SA   AN+     I VLT  G  A L+++ RP  PI ++   
Sbjct: 381 ATPLPMS----TLESLASSAVRTANKAKAKLIIVLTRGGTTAKLVAKYRPAVPILSVVVP 440

Query: 518 -----------TDDDSTRMALNLQWGVFPL------RIDLSEDIEANISRGIEVVKSKGL 572
                      +D+   R +L +  G+ P+      +   SE  E  I   ++    KGL
Sbjct: 441 VFTSDTFNWSCSDESPARHSL-IYRGLIPVLGEGSAKATDSESTEEIIESALKSATEKGL 500

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008454424.10.0e+00100.00PREDICTED: plastidial pyruvate kinase 1, chloroplastic-like [Cucumis melo] >ADN3... [more]
KAA0044313.10.0e+0099.83plastidial pyruvate kinase 1 [Cucumis melo var. makuwa][more]
XP_004150267.10.0e+0097.08plastidial pyruvate kinase 1, chloroplastic [Cucumis sativus] >KGN52735.1 hypoth... [more]
XP_038905899.12.4e-30692.96plastidial pyruvate kinase 1, chloroplastic-like [Benincasa hispida][more]
XP_023553283.12.3e-30492.43plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LIK01.0e-16960.16Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q431171.3e-16959.06Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Q405453.7e-16457.43Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q405463.1e-8640.26Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9FLW93.2e-8334.35Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3E0J90.0e+00100.00Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G0035... [more]
E5GBH70.0e+00100.00Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3BYK30.0e+00100.00Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103494833 PE=3 SV=1[more]
A0A5A7TLC20.0e+0099.83Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00350... [more]
A0A0A0KSY40.0e+0097.08Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000560 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G22960.17.1e-17160.16Pyruvate kinase family protein [more]
AT5G52920.12.3e-8434.35plastidic pyruvate kinase beta subunit 1 [more]
AT1G32440.11.9e-8338.82plastidial pyruvate kinase 3 [more]
AT3G04050.11.5e-5931.46Pyruvate kinase family protein [more]
AT5G63680.19.5e-5931.74Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 318..344
score: 52.93
coord: 345..369
score: 49.11
coord: 395..413
score: 54.25
coord: 154..170
score: 22.71
coord: 370..394
score: 57.66
coord: 287..301
score: 47.28
coord: 414..430
score: 35.11
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 97..568
e-value: 1.2E-123
score: 411.1
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 40..581
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 464..564
e-value: 1.0E-21
score: 77.3
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 96..434
e-value: 6.0E-86
score: 288.5
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 432..577
e-value: 1.1E-29
score: 104.9
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 460..563
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 166..268
e-value: 9.2E-109
score: 365.2
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 99..430
e-value: 9.2E-109
score: 365.2
NoneNo IPR availablePANTHERPTHR11817:SF21PYRUVATE KINASEcoord: 40..581
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 316..328
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 91..437
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 167..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C018027.1MELO3C018027.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
molecular_function GO:0003824 catalytic activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity