MELO3C017850 (gene) Melon (DHL92) v4

Overview
NameMELO3C017850
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionProtein NUCLEAR FUSION DEFECTIVE 4
Locationchr07: 25322006 .. 25326006 (-)
RNA-Seq ExpressionMELO3C017850
SyntenyMELO3C017850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGAAACTGGTTTTGAAAGGGGGAACTAGGCCGCCATGGGTTGGCTTAGCTGCTGCTGTTTGGGTTCAAATAGCTGGTGGTTCTTCTTATAACTTCTCTCTTTATTCACACTTGCTTAAATCTGTTCTGGGTTTGAATCAGCAGCAGCTTACTGTGTTGGGTGTGGCTAATGACATTGGAGAGAGTATGGGTTTGGTTCCTGGCCTCGCTTGTAACAGATTTCCTCCATGGGTTATACTTTTGTTTGGTGCTTTCTGTTGCTTTATAGGTTATGGAGCTATTTGGCTTGCTGTTAGTAGGACTGTCCCTAATCTGCCTTATTGGCTGGTGAGTTTAATGTTTATCTTTCTCTTTTTCAAGATCTTTTTTTCACGTTCTATGTGTTCTTCTGGCTTCCTGTTTTCAAGAACTTGTGGGTTTTCTTCTTTCTTGGTTGTTTGATGGTTTTGTTTGCTGTTCTTGGTTAAAGAAACGGTTTGGATTTTTTGTTGCTTTGTTATTAGCCTATGATCTAAGTCTTAATGTTTCTCTGTTAATGAATTCTTTGTTTATTGGTTTCTGGGAGATCTTGATTCAAAGCTGCTTACTAACTAGTTGTAACTTGATTGATAACTGATTAGCTAAATGCTGAGGGTTTTGATAGAGAAGTTTCTTTGTAAGATTTAATCCTTTTTTCGTTGATATGATACAAACAACTGACTAATAATGCAAAGGGTTGATAACAAATCCCCGCAATTCATCTAAATGAGATCATACGAAAATGATTTTTTCAAACAGTATAAAAAGAGGTTCCTATTATTCCCTTCCATCAACAGAAATAAGAAATAGTCTTACACTGAGGTCGAATGCGGTTCAATCAAGTTAAACAATCATGATATATGATTATTTCCCTTGTGGAAAAGATTGTTTATCCGAATCTTTGTGAGTACATAAGCCTCATTAAAGTTGAATGGAGTGAACATACGGCATTGGCTAACTAATGAGGTCAAATTGTCAATAATGTTGTCATTATTTCATTCTTTTCCCTGATTAAAGTTTTTCACCAAAAGCACAGAAGAAGAGATGGGTCATATGATTGTTGAAGAGATGGGTCAACATTTGAGCAACTTTTAGCAGAATTAGTTATTTCATTGGCATTCATCTATATATAATGACAGCTATCAAATTTTGAAATGAATGTATATGGCTGGTCAAAGTAATGCTGTCATCTTATTGATGCTAATGTGGTCATGCCATCTCTTGTAGTTGTAACTTACAAGCACAACCAGTCAGATGAAACTTCATATGTATTCAATGGATAAGGTTTAGTTGATTACAAGACGAATCCTACTATATGCAAGCAACTTTGGAACTCCGTCCTACTTGATTCCCAAGTTTCCTTCTAATTCTCACTTCCAACTTTTCATTTTGCGCCATTCTATTATCTACTAACTCTGCTACGCAACTTCAATTACATTTTCATATCTCCCATGGTTGATTGCTGAACGTCTGATTATAGTTTTTGATAGAAACTATAGAATTGGGACAGAAAACGTATCGATTGCTAGAAAAATACAATAAGAATACCTGAGTAAGTAGAGAAACTTGGAGACACCATTGAGAGAGAGTTGAAGCTTAAACTACCTAACATTTGTCTGCCTTCATCTTCATTAATTCTATTCATAATAACCATCCATGCCAGCCTATTCCTGATAATTTTCCTAACAGATTTACCCACTGAGCATAGGTAAAGCAGAAAAGAGGCCTAACATATATGATATATGTAGAAAATGGATGGGATATGAGTCATATGAGTGCTTACATATTCATCCATTTGCAAAGCTTAATTTCCTTTATATTTTCCTAGTCTTTGCAGAATTTTCCTTAGTTTTACTTCTTGATCTTGTATTCTTTCATTTTTTCTCAAATAACGTTAGGTTATTGATTTAAAAAGAAGAAAAAAGAAAGAAAGATATAGGCCATGTGCATTGAACTTTATTATATTGAATGTCATGGCAGCTATGGCTCACACATTGTATTGCTACAAATAGCAATGCATGGTTTGGAACAGCTGTGCTTGTAACCAACATGAGGAACTTCCCTCTCAGCAGAGGTACCGTTGCTGGCATTCTTAAAGGTTATGTTGGGCTTAGTGCTGCTGTATACACGGTGATATACAGTTTGGTGCTCAAAAAGTCTGCTTTGAATTTACTGTTGTTTCTTACAATTGGGATACCCATCTTGTGCCTAGCGTTGATGTACTTTGTTCGACCTTGTACTCCGGCTTCAACAGAGGATCCATCTGAGCGAGCTCATTTTCTTTTCACACAAGCTGCTTGTGTTTTGCTAGGTCTTTTTTTAGTATCGACCACAATTCTAGATGCAACAACGACCCCTAGTGATGCCGTTGGATACACCTTAGTTGCCATTATGGTTATCCTTTTAATGTCTCCTCTTGCAGTTCCCATAAAAATGACCATTTGTGCAAAAACAAAAACAGTTGGCCCTCGAGTTGATTCTTCAGAACCTTTGGCTTCTGGAGGAAGCGATTCTTCCCAAATTGAACCTTTATTGACACCATCTTCCTCAGCTACGAATCTCGGAAGTTTCTATGAGAATGATGATGCATCAGATGTTGAAACCCTTCTTGCTGTGGGAGAGGGGGCAATTCACAAGAAGAAAAGAAGACCTAAAAGAGGGGAAGATTTCAAGCTCCGTGAAGCTGTAATCAAAGCTGATTTCTGGCTGCTTTGGTTCTTGTACTTCCTCGGGGTCGGTCCTGGAATAACAGTTCTCAACAATTTGTCTCAAATTGGGATTTCGTTAGGCATTAATGACACAACATTGCTATTGGCTCTTTTCAGCTTCTGCAATTTTGTTGGTCGTTTAGGCTCCGGTGTAATTTCTGAACACTTTGTCAGGTAAATGCTTGTACATAACAGATCACTGAATTAATAACTTGTTCCAACTTTTGTCAATAGGGTATCTCAGAAACTGCTCCCTTTCTTTGTCAAGTTTTGGGGGTCTTAAGACTTCCAAATCTTCAAACTCAACTATCGAAGAGTAATTTCAGAGTTAACCTGGAGATTTTTCCAAATTACTTTGACTTTATGTTCTGAGTATCTAGTGATTTTCTCTGAAGGTGCTCCATGTGTCAAATGCTCATCAGTTCGGTACTTTTTGGTCATGCAGGTCAAGAATGATACCTCGCTCGCTATGGATGATGTTTGCACTCGTACTCATGTCTATAGCATTCCTCCTGTATGCCTCCGGCCTCACAATTACTCTCTACATCGCCACTGGTCTCACTGGAATCAGTTACGGAGTTCTTTATTCTATGATGGTCCCTCTTGCATCTGAGATTTTCGGCCTAAAGAATTTTGGCGTCATCTTCAACTTCATGCAGCTGGGGAATCCAATAGGAGCAGTCCTTTTCTCAGTTCTACTCACCAGTACTTTATACGACAAAGAAGCTGCTATGCAAGGTTCGATAACCTGCATAGGCCAACAGTGCTTCAGGACGACTTTCTTCATCCTTTCGGGCGTCGCTGGGTTGGGTTCAATCGTGAGCTTGATTCTGACTATCAGATTAAGGCCAGTGTATCAAATGCTTTATGCTGGTGGCTCGTTCCGCTTGCCTCAAAGTTCAGGTCATTGATGTTTTGAAGATGGCTTGAAACATCGTGCTACATTCGTCGGTGCGATGCTTTAACTTAAGAGTTGAAAAATCTGCAGCTTGTTTTTGCAGCATAATATCTTTTGACAAGTGATAAATTTTGTGTAACTTGAGAATTTCTCTATAGGCTTTGGCCAACTTGTATTCTCTGAGTTGGATAACTAATGTGTGTTAGGGTTCTTTTCTCTTTTCTTTTCTTTTTCAATAATGTATGTTGTGTTTGTTACCCTTGTGCATTAAACACAATGTCAAAGGCCTTTTTCTTTTAGGTACAAAATACATCAGTATGCTGTTTGATTCAATAATAATGATAATAATAACAATTTAGTATGTAAAATTTGAC

mRNA sequence

ATGCCGAAACTGGTTTTGAAAGGGGGAACTAGGCCGCCATGGGTTGGCTTAGCTGCTGCTGTTTGGGTTCAAATAGCTGGTGGTTCTTCTTATAACTTCTCTCTTTATTCACACTTGCTTAAATCTGTTCTGGGTTTGAATCAGCAGCAGCTTACTGTGTTGGGTGTGGCTAATGACATTGGAGAGAGTATGGGTTTGGTTCCTGGCCTCGCTTGTAACAGATTTCCTCCATGGGTTATACTTTTGTTTGGTTATGGAGCTATTTGGCTTGCTGTTAGTAGGACTGTCCCTAATCTGCCTTATTGGCTGCTATGGCTCACACATTGTATTGCTACAAATAGCAATGCATGGTTTGGAACAGCTGTGCTTGTAACCAACATGAGGAACTTCCCTCTCAGCAGAGGTACCGTTGCTGGCATTCTTAAAGGTTATGTTGGGCTTAGTGCTGCTGTATACACGGTGATATACAGTTTGGTGCTCAAAAAGTCTGCTTTGAATTTACTGTTGTTTCTTACAATTGGGATACCCATCTTGTGCCTAGCGTTGATGTACTTTGTTCGACCTTGTACTCCGGCTTCAACAGAGGATCCATCTGAGCGAGCTCATTTTCTTTTCACACAAGCTGCTTGTGTTTTGCTAGGTCTTTTTTTAGTATCGACCACAATTCTAGATGCAACAACGACCCCTAGTGATGCCGTTGGATACACCTTAGTTGCCATTATGGTTATCCTTTTAATGTCTCCTCTTGCAGTTCCCATAAAAATGACCATTTGTGCAAAAACAAAAACAGTTGGCCCTCGAGTTGATTCTTCAGAACCTTTGGCTTCTGGAGGAAGCGATTCTTCCCAAATTGAACCTTTATTGACACCATCTTCCTCAGCTACGAATCTCGGAAGTTTCTATGAGAATGATGATGCATCAGATGTTGAAACCCTTCTTGCTGTGGGAGAGGGGGCAATTCACAAGAAGAAAAGAAGACCTAAAAGAGGGGAAGATTTCAAGCTCCGTGAAGCTGTAATCAAAGCTGATTTCTGGCTGCTTTGGTTCTTGTACTTCCTCGGGGTCGGTCCTGGAATAACAGTTCTCAACAATTTGTCTCAAATTGGGATTTCGTTAGGCATTAATGACACAACATTGCTATTGGCTCTTTTCAGCTTCTGCAATTTTGTTGGTCGTTTAGGCTCCGGTGTAATTTCTGAACACTTTGTCAGGTCAAGAATGATACCTCGCTCGCTATGGATGATGTTTGCACTCGTACTCATGTCTATAGCATTCCTCCTGTATGCCTCCGGCCTCACAATTACTCTCTACATCGCCACTGGTCTCACTGGAATCAGTTACGGAGTTCTTTATTCTATGATGGTCCCTCTTGCATCTGAGATTTTCGGCCTAAAGAATTTTGGCGTCATCTTCAACTTCATGCAGCTGGGGAATCCAATAGGAGCAGTCCTTTTCTCAGTTCTACTCACCAGTACTTTATACGACAAAGAAGCTGCTATGCAAGGTTCGATAACCTGCATAGGCCAACAGTGCTTCAGGACGACTTTCTTCATCCTTTCGGGCGTCGCTGGGTTGGGTTCAATCGTGAGCTTGATTCTGACTATCAGATTAAGGCCAGTGTATCAAATGCTTTATGCTGGTGGCTCGTTCCGCTTGCCTCAAAGTTCAGGTCATTGATGTTTTGAAGATGGCTTGAAACATCGTGCTACATTCGTCGGTGCGATGCTTTAACTTAAGAGTTGAAAAATCTGCAGCTTGTTTTTGCAGCATAATATCTTTTGACAAGTGATAAATTTTGTGTAACTTGAGAATTTCTCTATAGGCTTTGGCCAACTTGTATTCTCTGAGTTGGATAACTAATGTGTGTTAGGGTTCTTTTCTCTTTTCTTTTCTTTTTCAATAATGTATGTTGTGTTTGTTACCCTTGTGCATTAAACACAATGTCAAAGGCCTTTTTCTTTTAGGTACAAAATACATCAGTATGCTGTTTGATTCAATAATAATGATAATAATAACAATTTAGTATGTAAAATTTGAC

Coding sequence (CDS)

ATGCCGAAACTGGTTTTGAAAGGGGGAACTAGGCCGCCATGGGTTGGCTTAGCTGCTGCTGTTTGGGTTCAAATAGCTGGTGGTTCTTCTTATAACTTCTCTCTTTATTCACACTTGCTTAAATCTGTTCTGGGTTTGAATCAGCAGCAGCTTACTGTGTTGGGTGTGGCTAATGACATTGGAGAGAGTATGGGTTTGGTTCCTGGCCTCGCTTGTAACAGATTTCCTCCATGGGTTATACTTTTGTTTGGTTATGGAGCTATTTGGCTTGCTGTTAGTAGGACTGTCCCTAATCTGCCTTATTGGCTGCTATGGCTCACACATTGTATTGCTACAAATAGCAATGCATGGTTTGGAACAGCTGTGCTTGTAACCAACATGAGGAACTTCCCTCTCAGCAGAGGTACCGTTGCTGGCATTCTTAAAGGTTATGTTGGGCTTAGTGCTGCTGTATACACGGTGATATACAGTTTGGTGCTCAAAAAGTCTGCTTTGAATTTACTGTTGTTTCTTACAATTGGGATACCCATCTTGTGCCTAGCGTTGATGTACTTTGTTCGACCTTGTACTCCGGCTTCAACAGAGGATCCATCTGAGCGAGCTCATTTTCTTTTCACACAAGCTGCTTGTGTTTTGCTAGGTCTTTTTTTAGTATCGACCACAATTCTAGATGCAACAACGACCCCTAGTGATGCCGTTGGATACACCTTAGTTGCCATTATGGTTATCCTTTTAATGTCTCCTCTTGCAGTTCCCATAAAAATGACCATTTGTGCAAAAACAAAAACAGTTGGCCCTCGAGTTGATTCTTCAGAACCTTTGGCTTCTGGAGGAAGCGATTCTTCCCAAATTGAACCTTTATTGACACCATCTTCCTCAGCTACGAATCTCGGAAGTTTCTATGAGAATGATGATGCATCAGATGTTGAAACCCTTCTTGCTGTGGGAGAGGGGGCAATTCACAAGAAGAAAAGAAGACCTAAAAGAGGGGAAGATTTCAAGCTCCGTGAAGCTGTAATCAAAGCTGATTTCTGGCTGCTTTGGTTCTTGTACTTCCTCGGGGTCGGTCCTGGAATAACAGTTCTCAACAATTTGTCTCAAATTGGGATTTCGTTAGGCATTAATGACACAACATTGCTATTGGCTCTTTTCAGCTTCTGCAATTTTGTTGGTCGTTTAGGCTCCGGTGTAATTTCTGAACACTTTGTCAGGTCAAGAATGATACCTCGCTCGCTATGGATGATGTTTGCACTCGTACTCATGTCTATAGCATTCCTCCTGTATGCCTCCGGCCTCACAATTACTCTCTACATCGCCACTGGTCTCACTGGAATCAGTTACGGAGTTCTTTATTCTATGATGGTCCCTCTTGCATCTGAGATTTTCGGCCTAAAGAATTTTGGCGTCATCTTCAACTTCATGCAGCTGGGGAATCCAATAGGAGCAGTCCTTTTCTCAGTTCTACTCACCAGTACTTTATACGACAAAGAAGCTGCTATGCAAGGTTCGATAACCTGCATAGGCCAACAGTGCTTCAGGACGACTTTCTTCATCCTTTCGGGCGTCGCTGGGTTGGGTTCAATCGTGAGCTTGATTCTGACTATCAGATTAAGGCCAGTGTATCAAATGCTTTATGCTGGTGGCTCGTTCCGCTTGCCTCAAAGTTCAGGTCATTGA

Protein sequence

MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESMGLVPGLACNRFPPWVILLFGYGAIWLAVSRTVPNLPYWLLWLTHCIATNSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTIGIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSSATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFLGVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLWMMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLILTIRLRPVYQMLYAGGSFRLPQSSGH
Homology
BLAST of MELO3C017850 vs. NCBI nr
Match: TYK29592.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1067.8 bits (2760), Expect = 3.3e-308
Identity = 558/565 (98.76%), Postives = 558/565 (98.76%), Query Frame = 0

Query: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLF-------GYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESMGLVPGLACNRFPPWVILLF       GYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180

Query: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240
           GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240

Query: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300
           GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS
Sbjct: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300

Query: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360
           ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL
Sbjct: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360

Query: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420
           GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW
Sbjct: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420

Query: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480
           MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF
Sbjct: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480

Query: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540
           MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL
Sbjct: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540

Query: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 559
           TIRLRPVYQMLYAGGSFRLPQSSGH
Sbjct: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of MELO3C017850 vs. NCBI nr
Match: KAA0044465.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1063.1 bits (2748), Expect = 8.0e-307
Identity = 556/565 (98.41%), Postives = 556/565 (98.41%), Query Frame = 0

Query: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLF-------GYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESMGLVPGLACNRFPPWVILLF       GYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180

Query: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240
           GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILD TTTPSDAV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDGTTTPSDAV 240

Query: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300
           GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS
Sbjct: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300

Query: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360
           ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKL EAVIKADFWLLWFLYFL
Sbjct: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLCEAVIKADFWLLWFLYFL 360

Query: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420
           GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW
Sbjct: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420

Query: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480
           MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF
Sbjct: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480

Query: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540
           MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL
Sbjct: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540

Query: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 559
           TIRLRPVYQMLYAGGSFRLPQSSGH
Sbjct: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of MELO3C017850 vs. NCBI nr
Match: XP_008454196.2 (PREDICTED: LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo])

HSP 1 Score: 1062.8 bits (2747), Expect = 1.0e-306
Identity = 556/565 (98.41%), Postives = 556/565 (98.41%), Query Frame = 0

Query: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLF-------GYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESMGLVPGLACNRFPPWVILLF       GYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180

Query: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240
           GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLL  FLVSTTILDATTTPSDAV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLXSFLVSTTILDATTTPSDAV 240

Query: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300
           GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS
Sbjct: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300

Query: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360
           ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL
Sbjct: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360

Query: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420
           GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW
Sbjct: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420

Query: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480
           MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF
Sbjct: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480

Query: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540
           MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL
Sbjct: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540

Query: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 559
           TIRLRPVYQMLYAGGSFRLPQSSGH
Sbjct: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of MELO3C017850 vs. NCBI nr
Match: XP_004152187.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN52923.1 hypothetical protein Csa_014743 [Cucumis sativus])

HSP 1 Score: 1045.4 bits (2702), Expect = 1.7e-301
Identity = 544/565 (96.28%), Postives = 552/565 (97.70%), Query Frame = 0

Query: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLF-------GYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESMGL+PGLACN+FPPWVILLF       GYGAIWLAVSRTVPNLPYWLLWLTHC+ATN
Sbjct: 61  GESMGLIPGLACNKFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYWLLWLTHCVATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVL+KSALNLLLFL I
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLRKSALNLLLFLAI 180

Query: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240
           GIPILCLA+MYFVRPCTPAS+EDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV
Sbjct: 181 GIPILCLAMMYFVRPCTPASSEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240

Query: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300
           GYTLVAIMVILLMSPLAVPIKMTICA+TKT+GPRVDSSEPLASG SDSSQIEPLLTPSSS
Sbjct: 241 GYTLVAIMVILLMSPLAVPIKMTICARTKTLGPRVDSSEPLASGESDSSQIEPLLTPSSS 300

Query: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360
           ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL
Sbjct: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360

Query: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420
           GVGPGITVLNNLSQIGISLGIND TLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW
Sbjct: 361 GVGPGITVLNNLSQIGISLGINDATLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420

Query: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480
           MMFALVLMSIAFLLYAS LTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF
Sbjct: 421 MMFALVLMSIAFLLYASALTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480

Query: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540
           MQLGNPIGAVLFSVLLTSTLYD EAA QGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL
Sbjct: 481 MQLGNPIGAVLFSVLLTSTLYDIEAAKQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540

Query: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 559
           TIRLRPVYQMLYAGGSFRLPQSSGH
Sbjct: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of MELO3C017850 vs. NCBI nr
Match: XP_038896285.1 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 1013.4 bits (2619), Expect = 7.3e-292
Identity = 524/565 (92.74%), Postives = 542/565 (95.93%), Query Frame = 0

Query: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGG+RPPWVGLAAAVWVQIA GSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLF-------GYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESMGL+PGLACNRFPPWVILLF       GYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESMGLIPGLACNRFPPWVILLFGAFCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180
           SNAWFGTAVLVTNMRNFPLSRG+VAGILKGYVGLSAAVYTVIYS+VL+KSALNLLLFL I
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGSVAGILKGYVGLSAAVYTVIYSMVLRKSALNLLLFLAI 180

Query: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240
           GIPILCLALMYFVRPCTPAS+EDPSER+HFLFTQA+CVLL +FLVSTTI+DATTTPSDAV
Sbjct: 181 GIPILCLALMYFVRPCTPASSEDPSERSHFLFTQASCVLLAVFLVSTTIVDATTTPSDAV 240

Query: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300
            YTLVAIMVI LMSPLAVPIKMTICAKTK +G RVDS EPLASG SDSSQIEPLLTPSSS
Sbjct: 241 AYTLVAIMVIFLMSPLAVPIKMTICAKTKKLGSRVDSVEPLASGESDSSQIEPLLTPSSS 300

Query: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360
           ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYF 
Sbjct: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFF 360

Query: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420
           GVGPGITVLNNLSQIGISLG+NDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW
Sbjct: 361 GVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420

Query: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480
           MMFAL+LM+IAFLLYAS LT TLY+ATGL GISYGVLYSMMVPLASEIFGLKNFGVIFNF
Sbjct: 421 MMFALILMAIAFLLYASALTGTLYVATGLIGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480

Query: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540
           MQLGNPIGAVLFSVLL STLYDKEAA QGSITCIGQQCFR TFFILSGVAGLGSIVS+IL
Sbjct: 481 MQLGNPIGAVLFSVLLASTLYDKEAAKQGSITCIGQQCFRMTFFILSGVAGLGSIVSMIL 540

Query: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 559
           T+RLRPVYQMLY+GGSFRLPQSSGH
Sbjct: 541 TVRLRPVYQMLYSGGSFRLPQSSGH 565

BLAST of MELO3C017850 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 8.4e-49
Identity = 170/563 (30.20%), Postives = 277/563 (49.20%), Query Frame = 0

Query: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           +P L    G    W  L AA+W+Q + G++++FS YS  LKSVLG++Q +L  L VA+D+
Sbjct: 31  LPPLRTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDL 90

Query: 61  GESMGLVPGLACNRFPPWVIL-------LFGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           G++ G   G+A   FP  V+L         GYG  WL ++  +  LPY L++L   +A  
Sbjct: 91  GKAFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNII-TLPYSLVFLCCLLAGL 150

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180
           S  WF TA  +  +R+FP +R     +   + G+SAA+Y++ ++  +  S+ NL L L  
Sbjct: 151 SICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFN-AINPSSSNLYLLLNS 210

Query: 181 GIP-ILCLALMY--FVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPS 240
            +P ++  A +Y    +P    +T D   R H         +L +      +L +++T S
Sbjct: 211 LVPLVVSFAALYPVLTKPSLD-TTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSS 270

Query: 241 DAVGYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTP 300
             + +      V+LL+ PL  P  + + A+   + P +++     S G     I+ L   
Sbjct: 271 ARLNFIGA---VVLLVFPLCAP--LLVYARDYFL-PVINARLNHESSGYVMLNIDELKNQ 330

Query: 301 SSSATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFL 360
            +S ++   +     A +  T+               + G++   R  + + +FWL +  
Sbjct: 331 KTSVSSKTGYEHMGTAKEGNTV---------------RLGDEHSFRLLISRLEFWLYYIA 390

Query: 361 YFLGVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRM-IP 420
           YF G   G+   NNL QI  SLG N TT L+ ++S  +F GRL S   +  F+  R  + 
Sbjct: 391 YFCGGTIGLVYSNNLGQIAQSLGQNSTT-LVTIYSSFSFFGRLLSA--APDFMHKRFRLT 450

Query: 421 RSLWMMFALVLMSIAFLLYA--SGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNF 480
           R+ W   AL+   IAF L A  S     L  AT L G+S G +++  V + S++FG  + 
Sbjct: 451 RTGWFAIALLPTPIAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSV 510

Query: 481 GVIFNFMQLGNPIGAVLFSVLLTSTLYDKEAA------MQGSITCIGQQCFRTTFFILSG 540
           GV  N +    PIG++L+  +  S +Y+  A+      +  SI CIG+ C+  TF     
Sbjct: 511 GVNHNILITNIPIGSLLYGYIAAS-IYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGC 565

Query: 541 VAGLGSIVSLILTIRLRPVYQML 545
           ++ LG + SL L IR +PVY  L
Sbjct: 571 LSILGVVSSLSLYIRTKPVYHRL 565

BLAST of MELO3C017850 vs. ExPASy Swiss-Prot
Match: Q4IM48 (Probable transporter MCH1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 8.2e-04
Identity = 51/212 (24.06%), Postives = 94/212 (44.34%), Query Frame = 0

Query: 345 WLLWFLYFLGVGPGITVLNNLSQIGISL------GINDTTLL---LALFSFCNFVGRLGS 404
           W     + L VGPG   +NNL  I  +L      G +  T     +++F   N   R+  
Sbjct: 331 WPFALAFLLIVGPGEAFINNLGTIIGTLTPPEMEGWSHRTSAATHVSIFGITNTASRIFI 390

Query: 405 GVISE-----------------HFVRSRM-IPRSLWMMFALVLMSIAFLLYASGLTIT-- 464
           G +++                   V SR  I R  +M F   ++SI  L+ ASGL     
Sbjct: 391 GTLTDLLAPYPHTQHVQGPSTRSAVSSRFSISRVAFMAFFASMLSIGLLILASGLVQNHA 450

Query: 465 --LYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLTSTL 518
              ++ +GL G  YG ++S+   + + I+G++NF   +  + +    G+  + ++ ++T 
Sbjct: 451 ERFWLVSGLVGAGYGAIFSLTPLMVTIIWGVENFATNYGLIGMLPAAGSTFWGLVYSATY 510

BLAST of MELO3C017850 vs. ExPASy TrEMBL
Match: A0A5D3E0Y7 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002010 PE=4 SV=1)

HSP 1 Score: 1067.8 bits (2760), Expect = 1.6e-308
Identity = 558/565 (98.76%), Postives = 558/565 (98.76%), Query Frame = 0

Query: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLF-------GYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESMGLVPGLACNRFPPWVILLF       GYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180

Query: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240
           GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240

Query: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300
           GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS
Sbjct: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300

Query: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360
           ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL
Sbjct: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360

Query: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420
           GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW
Sbjct: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420

Query: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480
           MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF
Sbjct: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480

Query: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540
           MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL
Sbjct: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540

Query: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 559
           TIRLRPVYQMLYAGGSFRLPQSSGH
Sbjct: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of MELO3C017850 vs. ExPASy TrEMBL
Match: A0A5A7TSX4 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002010 PE=4 SV=1)

HSP 1 Score: 1063.1 bits (2748), Expect = 3.9e-307
Identity = 556/565 (98.41%), Postives = 556/565 (98.41%), Query Frame = 0

Query: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLF-------GYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESMGLVPGLACNRFPPWVILLF       GYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180

Query: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240
           GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILD TTTPSDAV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDGTTTPSDAV 240

Query: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300
           GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS
Sbjct: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300

Query: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360
           ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKL EAVIKADFWLLWFLYFL
Sbjct: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLCEAVIKADFWLLWFLYFL 360

Query: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420
           GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW
Sbjct: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420

Query: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480
           MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF
Sbjct: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480

Query: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540
           MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL
Sbjct: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540

Query: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 559
           TIRLRPVYQMLYAGGSFRLPQSSGH
Sbjct: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of MELO3C017850 vs. ExPASy TrEMBL
Match: A0A1S3BY60 (LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103494674 PE=4 SV=1)

HSP 1 Score: 1062.8 bits (2747), Expect = 5.0e-307
Identity = 556/565 (98.41%), Postives = 556/565 (98.41%), Query Frame = 0

Query: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLF-------GYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESMGLVPGLACNRFPPWVILLF       GYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180

Query: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240
           GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLL  FLVSTTILDATTTPSDAV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLXSFLVSTTILDATTTPSDAV 240

Query: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300
           GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS
Sbjct: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300

Query: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360
           ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL
Sbjct: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360

Query: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420
           GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW
Sbjct: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420

Query: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480
           MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF
Sbjct: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480

Query: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540
           MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL
Sbjct: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540

Query: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 559
           TIRLRPVYQMLYAGGSFRLPQSSGH
Sbjct: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of MELO3C017850 vs. ExPASy TrEMBL
Match: A0A0A0KTP3 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006300 PE=4 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 8.3e-302
Identity = 544/565 (96.28%), Postives = 552/565 (97.70%), Query Frame = 0

Query: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLF-------GYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GESMGL+PGLACN+FPPWVILLF       GYGAIWLAVSRTVPNLPYWLLWLTHC+ATN
Sbjct: 61  GESMGLIPGLACNKFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYWLLWLTHCVATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVL+KSALNLLLFL I
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLRKSALNLLLFLAI 180

Query: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240
           GIPILCLA+MYFVRPCTPAS+EDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV
Sbjct: 181 GIPILCLAMMYFVRPCTPASSEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240

Query: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300
           GYTLVAIMVILLMSPLAVPIKMTICA+TKT+GPRVDSSEPLASG SDSSQIEPLLTPSSS
Sbjct: 241 GYTLVAIMVILLMSPLAVPIKMTICARTKTLGPRVDSSEPLASGESDSSQIEPLLTPSSS 300

Query: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360
           ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL
Sbjct: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360

Query: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420
           GVGPGITVLNNLSQIGISLGIND TLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW
Sbjct: 361 GVGPGITVLNNLSQIGISLGINDATLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420

Query: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480
           MMFALVLMSIAFLLYAS LTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF
Sbjct: 421 MMFALVLMSIAFLLYASALTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480

Query: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540
           MQLGNPIGAVLFSVLLTSTLYD EAA QGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL
Sbjct: 481 MQLGNPIGAVLFSVLLTSTLYDIEAAKQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540

Query: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 559
           TIRLRPVYQMLYAGGSFRLPQSSGH
Sbjct: 541 TIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of MELO3C017850 vs. ExPASy TrEMBL
Match: A0A6J1GWT3 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111457872 PE=4 SV=1)

HSP 1 Score: 991.5 bits (2562), Expect = 1.4e-285
Identity = 510/566 (90.11%), Postives = 540/566 (95.41%), Query Frame = 0

Query: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           M KLVLKGG+RPPWVGLAAAVWVQIA GSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MSKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILL-------FGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GES+GL+PGLACNRFPPW +LL        GYGAIWLAVSRTVPNLPYWLLWLTHCIATN
Sbjct: 61  GESVGLLPGLACNRFPPWAVLLVGAVCCFVGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180
           SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYS+VL+KSAL+L+LFL I
Sbjct: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLRKSALDLVLFLAI 180

Query: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240
           GIPILCLALMYFVRPCTPASTEDPSER HFLFTQAACVLL +FLVSTTILDATTTPS+AV
Sbjct: 181 GIPILCLALMYFVRPCTPASTEDPSERNHFLFTQAACVLLAVFLVSTTILDATTTPSNAV 240

Query: 241 GYTLVAIMVILLMSPLAVPIKMTICA-KTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSS 300
            YTLVAIMV+LLMSPLAVPIKMTIC+ KTK +GPRVDS+EPLASG SDSSQIEPLLTPSS
Sbjct: 241 AYTLVAIMVVLLMSPLAVPIKMTICSVKTKKLGPRVDSTEPLASGESDSSQIEPLLTPSS 300

Query: 301 SATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYF 360
           SATNLGSFYEND++SDVETLLAVGEGAIHKKKR+PKRGEDFKLREAVIKADFWLLWFLYF
Sbjct: 301 SATNLGSFYENDESSDVETLLAVGEGAIHKKKRKPKRGEDFKLREAVIKADFWLLWFLYF 360

Query: 361 LGVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSL 420
           LGVGPGITVLNNLSQIGI+LG+NDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSL
Sbjct: 361 LGVGPGITVLNNLSQIGIALGVNDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSL 420

Query: 421 WMMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFN 480
           WMMFAL+LMS+AFLLYAS  T+TLY+ATGL GISYGVLY+MMVPLASEIFGLKNFGVIFN
Sbjct: 421 WMMFALMLMSVAFLLYASAHTVTLYVATGLMGISYGVLYAMMVPLASEIFGLKNFGVIFN 480

Query: 481 FMQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLI 540
           FMQLGNPIGAVLFSVLL STLYD EAA QGSITCIGQ+CFR TFF+LSGVAGLGSI+ +I
Sbjct: 481 FMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQECFRVTFFVLSGVAGLGSILCII 540

Query: 541 LTIRLRPVYQMLYAGGSFRLPQSSGH 559
           LT+RLRPVYQMLYAGGSFRLPQ+SGH
Sbjct: 541 LTVRLRPVYQMLYAGGSFRLPQNSGH 566

BLAST of MELO3C017850 vs. TAIR 10
Match: AT1G80530.1 (Major facilitator superfamily protein )

HSP 1 Score: 636.7 bits (1641), Expect = 1.7e-182
Identity = 328/566 (57.95%), Postives = 422/566 (74.56%), Query Frame = 0

Query: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           M KL  K G+RPPWVGLAAA WVQ++ GS   F LYS  LKSVLG +QQQ+T+LGVA D+
Sbjct: 1   MTKLAEKSGSRPPWVGLAAAAWVQVSAGSGSTFPLYSSALKSVLGFSQQQVTILGVACDL 60

Query: 61  GESMGLVPGLACNRFPPWVILL-------FGYGAIWLAVSRTVPNLPYWLLWLTHCIATN 120
           GE+MGL+PG A N+ PPW +LL        G+G +WL+VS+ V  LP+WLL++   +ATN
Sbjct: 61  GENMGLLPGYASNKLPPWSMLLIGASSCFLGFGVLWLSVSQIVLGLPFWLLFVALALATN 120

Query: 121 SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTI 180
           SN+WFGTA LVTNMRNFP+SRG VAG+LKGY+G+S A +TV++S+VL  SA++LLLFLT+
Sbjct: 121 SNSWFGTASLVTNMRNFPMSRGPVAGLLKGYIGISGAAFTVLFSMVLHHSAMDLLLFLTV 180

Query: 181 GIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAV 240
           GIP++CL +MYF+RPC PA+ EDPSE  +F F     +L   +LV TT+L         +
Sbjct: 181 GIPVICLTVMYFIRPCIPATGEDPSEPMYFAFLLVTSILFAAYLVVTTVLSEVFILPSIL 240

Query: 241 GYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSS 300
            Y LVAIMV+LL+SPLAVPIKMT+        P + SS+ LA    + +  EPLLTPS+S
Sbjct: 241 KYVLVAIMVLLLLSPLAVPIKMTLFRSNAKSSP-LGSSDNLAK--EEGTHEEPLLTPSTS 300

Query: 301 ATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 360
           A+NLG  +E DD SD+E LLA  EGA+ KKKR+P+RGEDFK  +  +KADFWLLWF+YFL
Sbjct: 301 ASNLGPIFEGDDESDMEILLAEAEGAV-KKKRKPRRGEDFKFGQVFVKADFWLLWFVYFL 360

Query: 361 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 420
           G+G G+TV NNL+QIG + GI DTT+LL LFSF NF+GRL SG ISEHFVRSR +PR+LW
Sbjct: 361 GMGSGVTVSNNLAQIGFAFGIKDTTILLCLFSFFNFIGRLASGAISEHFVRSRTLPRTLW 420

Query: 421 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 480
           M  A ++M   FLL+A  +  T+Y+AT L GI  G  + + +   SE+FGL++FG+ FNF
Sbjct: 421 MGAAQLVMVFTFLLFAMAIDHTIYVATALIGICMGFQF-LSIATISELFGLRHFGINFNF 480

Query: 481 MQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFRTTFFILSGVAGLGSIVSLIL 540
           + LGNP+GA +FS +L   +YDKEA  QG +TCIG  CFR TF +L+GV GLG+++S+IL
Sbjct: 481 ILLGNPLGATIFSAILAGYIYDKEADKQGKMTCIGPDCFRVTFLVLAGVCGLGTLLSIIL 540

Query: 541 TIRLRPVYQMLYAGGSFRL-PQSSGH 559
           T+R+RPVYQ LYA GSFRL PQS+GH
Sbjct: 541 TVRIRPVYQALYASGSFRLQPQSTGH 561

BLAST of MELO3C017850 vs. TAIR 10
Match: AT4G34950.1 (Major facilitator superfamily protein )

HSP 1 Score: 335.9 bits (860), Expect = 6.3e-92
Identity = 208/555 (37.48%), Postives = 317/555 (57.12%), Query Frame = 0

Query: 14  WVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESMGLVPGLACN 73
           W+G   AVWVQ   G++Y FS YS  LKS++ L Q +L  L VA D+G++ G++ GLA +
Sbjct: 17  WLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLTQLELNSLSVAKDVGKAFGILAGLASD 76

Query: 74  RFPPWVIL-------LFGYGAIWLAVSRTVPNLPYWLLWLTHCIATNSNAWFGTAVLVTN 133
           R    VIL       L GYG  WL VSRT+  +PYW + +  C+  NS  W  TAVLVT 
Sbjct: 77  RLSTPVILLIGSFEGLLGYGVQWLVVSRTIQPIPYWQMCVFLCMGGNSTTWMNTAVLVTC 136

Query: 134 MRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTIGIPILCLALMYFV 193
           +RNF  +RG V+GILKGYVGLS A++T + + +      + L+ L++    +CL  ++F+
Sbjct: 137 IRNFRRNRGPVSGILKGYVGLSTAIFTDLCNALFSSDPASFLVLLSVVPFAVCLTAVFFL 196

Query: 194 RPCTPAST--EDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAVGYTLVAIMVIL 253
           R   P++T  ED  E  +F       V++ ++L S  I+   T    A      +I++IL
Sbjct: 197 REIPPSTTFAEDNEESKYFAVFNIVAVVVAVYLQSYDIIGIKT---GAFSIAFASILLIL 256

Query: 254 LMSPLAVPIKMTICAKT---KTVGPRVDSSEPLASGGSDSSQIEPLLTPSSSATN----- 313
           L SP+AVP    I +K    + V  R+D  EPL   GS+    E ++  +++A N     
Sbjct: 257 LASPVAVPFHAFIRSKVHDEQDVEGRID--EPLLRSGSEIEVEETIVGAAAAADNELPPS 316

Query: 314 ---LGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFL 373
              L +  E +  + V T           +K+RP  GE+  + EA++  DFW+L+  +  
Sbjct: 317 LKPLSNEEEENHGTIVTT-----------EKKRPVLGEEHTIMEAMLTVDFWVLFVSFLC 376

Query: 374 GVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLW 433
           GVG G+ V+NN+ QIG++LG  D ++ +++ S   F GR+ SG ISEHF++    PR LW
Sbjct: 377 GVGTGLAVMNNMGQIGLALGYTDVSIFVSMTSIWGFFGRILSGTISEHFIKKAGTPRPLW 436

Query: 434 MMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNF 493
              A ++M++ +LL A  L  +LYI + + G+ YGV  ++ VP ASE+FGLK +G+I+N 
Sbjct: 437 NAAAQIIMAVGYLLMALALPGSLYIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNI 496

Query: 494 MQLGNPIGAVLFSVLLTSTLYDKEAAMQ--GSITCIGQQCFRTTFFILSGVAGLGSIVSL 547
           + L  P+G+ LFS LL   LYD EA     G  TC+G  CFR  F +++  + +G  + L
Sbjct: 497 LILNMPLGSFLFSGLLAGLLYDAEATPTPGGGNTCVGAHCFRIVFIVMAFASIIGVGLDL 555

BLAST of MELO3C017850 vs. TAIR 10
Match: AT2G16660.1 (Major facilitator superfamily protein )

HSP 1 Score: 327.8 bits (839), Expect = 1.7e-89
Identity = 198/544 (36.40%), Postives = 306/544 (56.25%), Query Frame = 0

Query: 14  WVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESMGLVPGLACN 73
           W+G   AVWVQ   G++Y FS YS  LKS++ LNQ +L  L VA D+G++ G++ GLA +
Sbjct: 15  WLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLNQLELNNLSVAKDVGKAFGILAGLASD 74

Query: 74  RFPPWVIL-------LFGYGAIWLAVSRTVPNLPYWLLWLTHCIATNSNAWFGTAVLVTN 133
           R P  VIL       L GYG  WL VSRT+  +PYW + +  C+  NS  W  TAVLVT 
Sbjct: 75  RLPTPVILLIGCFEGLLGYGVQWLVVSRTIQPIPYWQMCIFLCMGGNSTTWMNTAVLVTC 134

Query: 134 MRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTIGIPILCLALMYFV 193
           +RNF  +RG V+GILKGYVGLS A++T + + +      + L+ L +    +CL  ++F+
Sbjct: 135 IRNFRRNRGPVSGILKGYVGLSTAIFTDLCTALFSNDPASFLVLLAVVPFAVCLTAVFFL 194

Query: 194 RPCTPAST--EDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAVGYTLVAIMVIL 253
           R   PAS+  E+  E  +F       V++ ++L S  I+   T           +I++ L
Sbjct: 195 REIPPASSAAEENEETRYFTIFNIVAVVVAVYLQSYDIIGIKT---GVFSVAFASILLFL 254

Query: 254 LMSPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSSATNLGSFYEND 313
           L SP+A+P    I  K+   G + D    +          EPLL    +A          
Sbjct: 255 LASPIAIPFHSFI--KSLNYGEQDDLEGRIQ---------EPLLRSEIAAAE-------- 314

Query: 314 DASDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFLGVGPGITVLNN 373
              +V  + AV      KK+++P  GED  + EAV+  DFW+L+  +  GVG G+ V+NN
Sbjct: 315 --KEVIVVAAVAAEEEEKKRKKPVLGEDHTIMEAVLTVDFWVLFVSFLCGVGTGLAVMNN 374

Query: 374 LSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLWMMFALVLMSIA 433
           + QIG++LG  + ++ +++ S   F GR+ SG +SE+F++    PR LW   + +LM++ 
Sbjct: 375 MGQIGLALGYTNVSIFVSMTSIWGFFGRILSGTLSEYFLKKAGTPRPLWNAASQILMAVG 434

Query: 434 FLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNFMQLGNPIGAVL 493
           ++L A  +  +LYI + + G+ YGV  ++ VP ASE+FGLK +G+I+N + L  P+G+ L
Sbjct: 435 YILMALAVPNSLYIGSMVVGVCYGVRLAITVPTASELFGLKYYGLIYNILVLNLPLGSFL 494

Query: 494 FSVLLTSTLYDKEAAMQ--GSITCIGQQCFRTTFFILSGVAGLGSIVSLILTIRLRPVYQ 547
           FS LL   LYD EA     G  TC+G  C+R  F +++  + +G  + L+L  R + +Y 
Sbjct: 495 FSGLLAGFLYDAEATPTPGGGNTCVGAHCYRLIFIVMALASVIGVGLDLVLAYRTKEIYA 534

BLAST of MELO3C017850 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 319.3 bits (817), Expect = 6.1e-87
Identity = 198/566 (34.98%), Postives = 306/566 (54.06%), Query Frame = 0

Query: 14  WVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESMGLVPGLACN 73
           W+   AA+W+Q   G  Y F   S ++KS L  NQ++L+ LGVA D+G+S+G + G    
Sbjct: 17  WLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLSE 76

Query: 74  RFPPWVIL-------LFGYGAIWLAVSRTVPNLPYWLLWLTHCIATNSNAWFGTAVLVTN 133
             P W  L       L GYG +WL V+   P LP W + +   +  N   +F T  LV+ 
Sbjct: 77  ILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVSG 136

Query: 134 MRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTIGIPILCLALMYFV 193
           ++NFP SRG V GILKG+ GL  A+ + IY+++   +  +L+L + +   ++ + LM+F+
Sbjct: 137 VQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCLMFFI 196

Query: 194 RPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAVGYTLVAIMVILLM 253
           RP        P++ A F F    C+LL  +L+S  ++      S  V      ++ ++L+
Sbjct: 197 RPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVILV 256

Query: 254 SPLAVPIKMTICAKTKTVGPRVDSSEPLASGGSDSSQIEPLLTPSSSATNLGSFYENDDA 313
            P+ VPI  +    T+T  P     EPL     D  Q   L TP    + +    E++  
Sbjct: 257 VPILVPIMTSFF--TETNEPDDTIEEPLVPKRED--QEPGLQTPDLILSEV----EDEKP 316

Query: 314 SDVETL-----------------LAVGEGAIHKKKRR-PKRGEDFKLREAVIKADFWLLW 373
            DV+ L                  A  EGA+   +RR P RGEDF L +A++KADFWL++
Sbjct: 317 KDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIF 376

Query: 374 FLYFLGVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMI 433
           F   LG G G+TV++NL Q+  SLG ++T +L+++ S  NF+GR+G G  SE  VR    
Sbjct: 377 FSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVLVSMISIWNFLGRIGGGYFSELVVRDYAY 436

Query: 434 PRSLWMMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKNFG 493
           PR + M  A ++MS+  + +A G    +YI T L G+ YG  ++++   ASE+FGLK FG
Sbjct: 437 PRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFG 496

Query: 494 VIFNFMQLGNPIGAVLFSVLLTSTLYDKEAAMQG---------SITCIGQQCFRTTFFIL 546
            ++NF+ L NP G+++FS ++ S++YD+EA  Q          ++ C G  CF  T  I+
Sbjct: 497 ALYNFLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIM 556

BLAST of MELO3C017850 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 312.0 bits (798), Expect = 9.7e-85
Identity = 195/568 (34.33%), Postives = 305/568 (53.70%), Query Frame = 0

Query: 14  WVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESMGLVPGLACN 73
           W+   AA+W+Q   G  Y F   S ++KS L  NQ+QL+ LGVA D+G+S+G + G    
Sbjct: 17  WLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAGTLSE 76

Query: 74  RFPPWVIL-------LFGYGAIWLAVSRTVPNLPYWLLWLTHCIATNSNAWFGTAVLVTN 133
             P W  L       L GYG +WL V+   P LP W + +   +  N   +F TA LV+ 
Sbjct: 77  ILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYFNTAALVSG 136

Query: 134 MRNFPLSRGTVAGILKGYVGLSAAVYTVIYSLVLKKSALNLLLFLTIGIPILCLALMYFV 193
           ++NFP SRG V GILKG+ GL  A+ + +Y+++      +L+  + +   ++ + LM+F+
Sbjct: 137 VQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPSVVVVPLMFFI 196

Query: 194 RPCTPASTEDPSERAHFLFTQAACVLLGLFLVSTTILDATTTPSDAVGYTLVAIMVILLM 253
           RP         S+   F    A C+LL  +L++  +++     S ++      ++  +L+
Sbjct: 197 RPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAFTVVLFAILL 256

Query: 254 SPLAVPIKMTICAKTKTVGPRVDSSEPLASG--GSDSSQIEPLLTPSSSATNLGSFYEND 313
            P+ +PI  T C  T +  P     EPL     G D  Q     TP      + S  E++
Sbjct: 257 VPIFIPI-ATSCF-TASTDPCDTLEEPLLGDQQGQDPGQ---STTPDHGPELIFSEVEDE 316

Query: 314 DASDVETLLAV-----------------GEGAIHKKKRR-PKRGEDFKLREAVIKADFWL 373
              +V+ L AV                  EGA+  K+RR P RGEDF L +A++KADFWL
Sbjct: 317 KPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKADFWL 376

Query: 374 LWFLYFLGVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRLGSGVISEHFVRSR 433
           ++F   LG G G+TV++NL Q+  SLG ++T + +++ S  NF+GR+G G  SE  VR  
Sbjct: 377 IFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWNFLGRIGGGYFSELIVRDY 436

Query: 434 MIPRSLWMMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSMMVPLASEIFGLKN 493
             PR + +  A ++MS+  + +A G    ++I T L G+ YG  ++++   ASE+FGLK 
Sbjct: 437 AYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASELFGLKK 496

Query: 494 FGVIFNFMQLGNPIGAVLFSVLLTSTLYDKEAAMQGS---------ITCIGQQCFRTTFF 546
           FG ++NF+ L NP G+++FS L+ S++YD+EA  Q           + C G  C+  T  
Sbjct: 497 FGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDVLRCRGSICYFLTSL 556

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK29592.13.3e-30898.76protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa][more]
KAA0044465.18.0e-30798.41protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa][more]
XP_008454196.21.0e-30698.41PREDICTED: LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo... [more]
XP_004152187.11.7e-30196.28protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN52923.1 hypothetical pr... [more]
XP_038896285.17.3e-29292.74protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
F4I9E18.4e-4930.20Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q4IM488.2e-0424.06Probable transporter MCH1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 ... [more]
Match NameE-valueIdentityDescription
A0A5D3E0Y71.6e-30898.76Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A5A7TSX43.9e-30798.41Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3BY605.0e-30798.41LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 ... [more]
A0A0A0KTP38.3e-30296.28Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G00630... [more]
A0A6J1GWT31.4e-28590.11protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111457872... [more]
Match NameE-valueIdentityDescription
AT1G80530.11.7e-18257.95Major facilitator superfamily protein [more]
AT4G34950.16.3e-9237.48Major facilitator superfamily protein [more]
AT2G16660.11.7e-8936.40Major facilitator superfamily protein [more]
AT5G14120.16.1e-8734.98Major facilitator superfamily protein [more]
AT3G01930.29.7e-8534.33Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 13..253
e-value: 6.1E-83
score: 278.1
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 339..541
e-value: 1.9E-10
score: 42.5
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 11..192
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 325..539
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 265..288
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 274..288
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 3..558
NoneNo IPR availablePANTHERPTHR21576:SF97MAJOR FACILITATOR SUPERFAMILY PROTEINcoord: 3..558
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 12..526
e-value: 5.90582E-101
score: 308.411

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C017850.1MELO3C017850.1mRNA