MELO3C017475.jh1 (gene) Melon (Harukei-3) v1.41

Overview


Name	MELO3C017475.jh1
Type	gene
Organism	Cucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Description	Nicotianamine synthase-like
Location	chr02: 20640548 .. 20641402 (-)
RNA-Seq Expression	MELO3C017475.jh1
Synteny	MELO3C017475.jh1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidestart_codonstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCCCTCCAATTTCATGGCATGGAAGCCGAAATCTCCGCAGCGTTTCTGGTAGATCGAATCATTGACCTTCATCAAAGCATCTTGAAGCTTGAAACCTTAAGGCCATGCAAGAAAGTGAACAACCTCTTCACCAACCTAGTGAACTTATGCATTTTGCCTTGTTCAATAGATGTCTCCACGTTGCCACCAAATTTACAAGCCATTCGTGAGAGCCTCATAATTCTCTGTGGCCAAGCTGAAGGCTTACTCGAGTTAGAATTCTCAACTTTGCTAAGCAAAATCCCAAAGCCTCTCAACAACCTCACTTTATTTCCATATTACCAAAACTACGTCAAATTAGCCAACCTTGAAAACAAAATTCTAAATGACAATGGAATCGTCAATCCTAAGAAGGTAGCTTTTGTGGGGTCAGGTCCTTTGCCTCTAACTTCAATTATTATGGCCATGCAACACATGAAGGGCACATATTTTGATAACTACGACGTAGACAGTGTAGCGAATGATGTAGCGAGAAAGATCGTGGGGTCGGATGCTGAGCTGGAGGGGAGAATGAAGTTTTGCTCTAGTGATATTGTTGAGGTAAAAGAAGAGCTTGGAGGATATGATTGTGTGTTTTTGGCTGCTCTCGTGGGGATGAATAAGGAAGAAAAAGTGAAGATTGTAAGGCATTTAAGGAAATATATGAAAGAAGGTGGGATTTTGTTGGTGAGAAGTGCTCAAGGAGGGAGAGCGTTTTTGTATCCTGTGGTTAAAGTTGGAGATTTGGTTGGGTTCGAGATTCTCTCTATTTTTCATCCAACTGATGATGTTGTCAATTCAGTTATCTTAGCTCGTAAGCAAACTAGCTAG

mRNA sequence

ATGGCAGCCCTCCAATTTCATGGCATGGAAGCCGAAATCTCCGCAGCGTTTCTGGTAGATCGAATCATTGACCTTCATCAAAGCATCTTGAAGCTTGAAACCTTAAGGCCATGCAAGAAAGTGAACAACCTCTTCACCAACCTAGTGAACTTATGCATTTTGCCTTGTTCAATAGATGTCTCCACGTTGCCACCAAATTTACAAGCCATTCGTGAGAGCCTCATAATTCTCTGTGGCCAAGCTGAAGGCTTACTCGAGTTAGAATTCTCAACTTTGCTAAGCAAAATCCCAAAGCCTCTCAACAACCTCACTTTATTTCCATATTACCAAAACTACGTCAAATTAGCCAACCTTGAAAACAAAATTCTAAATGACAATGGAATCGTCAATCCTAAGAAGGTAGCTTTTGTGGGGTCAGGTCCTTTGCCTCTAACTTCAATTATTATGGCCATGCAACACATGAAGGGCACATATTTTGATAACTACGACGTAGACAGTGTAGCGAATGATGTAGCGAGAAAGATCGTGGGGTCGGATGCTGAGCTGGAGGGGAGAATGAAGTTTTGCTCTAGTGATATTGTTGAGGTAAAAGAAGAGCTTGGAGGATATGATTGTGTGTTTTTGGCTGCTCTCGTGGGGATGAATAAGGAAGAAAAAGTGAAGATTGTAAGGCATTTAAGGAAATATATGAAAGAAGGTGGGATTTTGTTGGTGAGAAGTGCTCAAGGAGGGAGAGCGTTTTTGTATCCTGTGGTTAAAGTTGGAGATTTGGTTGGGTTCGAGATTCTCTCTATTTTTCATCCAACTGATGATGTTGTCAATTCAGTTATCTTAGCTCGTAAGCAAACTAGCTAG

Coding sequence (CDS)

ATGGCAGCCCTCCAATTTCATGGCATGGAAGCCGAAATCTCCGCAGCGTTTCTGGTAGATCGAATCATTGACCTTCATCAAAGCATCTTGAAGCTTGAAACCTTAAGGCCATGCAAGAAAGTGAACAACCTCTTCACCAACCTAGTGAACTTATGCATTTTGCCTTGTTCAATAGATGTCTCCACGTTGCCACCAAATTTACAAGCCATTCGTGAGAGCCTCATAATTCTCTGTGGCCAAGCTGAAGGCTTACTCGAGTTAGAATTCTCAACTTTGCTAAGCAAAATCCCAAAGCCTCTCAACAACCTCACTTTATTTCCATATTACCAAAACTACGTCAAATTAGCCAACCTTGAAAACAAAATTCTAAATGACAATGGAATCGTCAATCCTAAGAAGGTAGCTTTTGTGGGGTCAGGTCCTTTGCCTCTAACTTCAATTATTATGGCCATGCAACACATGAAGGGCACATATTTTGATAACTACGACGTAGACAGTGTAGCGAATGATGTAGCGAGAAAGATCGTGGGGTCGGATGCTGAGCTGGAGGGGAGAATGAAGTTTTGCTCTAGTGATATTGTTGAGGTAAAAGAAGAGCTTGGAGGATATGATTGTGTGTTTTTGGCTGCTCTCGTGGGGATGAATAAGGAAGAAAAAGTGAAGATTGTAAGGCATTTAAGGAAATATATGAAAGAAGGTGGGATTTTGTTGGTGAGAAGTGCTCAAGGAGGGAGAGCGTTTTTGTATCCTGTGGTTAAAGTTGGAGATTTGGTTGGGTTCGAGATTCTCTCTATTTTTCATCCAACTGATGATGTTGTCAATTCAGTTATCTTAGCTCGTAAGCAAACTAGCTAG

Protein sequence

MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDVSTLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLENKILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDAELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRSAQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARKQTS

Homology

[Close]

BLAST of MELO3C017475.jh1 vs. NCBI nr
Match: KAA0058291.1 (nicotianamine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 553 bits (1425), Expect = 2.48e-198
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0


Query: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120
           STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180
           KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240
           ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARKQTS 284
           AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARKQTS
Sbjct: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARKQTS 284

[Close]

BLAST of MELO3C017475.jh1 vs. NCBI nr
Match: XP_008453616.1 (PREDICTED: nicotianamine synthase-like [Cucumis melo] >TYK11847.1 nicotianamine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 551 bits (1421), Expect = 1.01e-197
Identity = 283/284 (99.65%), Postives = 284/284 (100.00%), Query Frame = 0


Query: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120
           STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180
           KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240
           ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARKQTS 284
           AQGGRAFLYPVV+VGDLVGFEILSIFHPTDDVVNSVILARKQTS
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARKQTS 284

[Close]

BLAST of MELO3C017475.jh1 vs. NCBI nr
Match: XP_004146360.2 (probable nicotianamine synthase 4 [Cucumis sativus] >KGN65443.1 hypothetical protein Csa_020027 [Cucumis sativus])

HSP 1 Score: 522 bits (1344), Expect = 5.72e-186
Identity = 265/281 (94.31%), Postives = 274/281 (97.51%), Query Frame = 0


Query: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ HG EAEISAAFLVDRIIDLH+SI KLETLRPCKKVNNLFTNLV LCILPCSIDV
Sbjct: 1   MAALQIHGTEAEISAAFLVDRIIDLHESISKLETLRPCKKVNNLFTNLVTLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120
           STLPPNLQ IRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNY+KLANLEN
Sbjct: 61  STLPPNLQVIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120

Query: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180
           KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGT+FDNYDVDSVANDVARKIVGSD+
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTHFDNYDVDSVANDVARKIVGSDS 180

Query: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240
           +LEGRMKFCSSDIV+VKEELGGYDCVFLAALVGMNKEEKVKI+RHLRKYMKEGGILLVRS
Sbjct: 181 DLEGRMKFCSSDIVDVKEELGGYDCVFLAALVGMNKEEKVKIIRHLRKYMKEGGILLVRS 240

Query: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARK 281
           A+GGRAFLYPVV+V DLVGFEILSIFHPTDDVVNSVIL RK
Sbjct: 241 AKGGRAFLYPVVEVEDLVGFEILSIFHPTDDVVNSVILTRK 281

[Close]

BLAST of MELO3C017475.jh1 vs. NCBI nr
Match: XP_038880344.1 (nicotianamine synthase-like [Benincasa hispida])

HSP 1 Score: 509 bits (1310), Expect = 8.69e-181
Identity = 256/281 (91.10%), Postives = 269/281 (95.73%), Query Frame = 0


Query: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ HG + EIS  FLVDRIIDLH+SI KLETL+PCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQIHGTKTEISTEFLVDRIIDLHESISKLETLKPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120
           STLPPNLQAIRESLI+LCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNY+KLANLEN
Sbjct: 61  STLPPNLQAIRESLIVLCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120

Query: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180
           KILNDNGI NPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYD+DSVANDVA KIVGSD 
Sbjct: 121 KILNDNGIFNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDIDSVANDVAGKIVGSDT 180

Query: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240
           ELEGRMKF + DIVEVKEELGGYDCVFLAALVGM KE+KVKI+RHLRKYMKEGGIL+VRS
Sbjct: 181 ELEGRMKFWTRDIVEVKEELGGYDCVFLAALVGMKKEDKVKIIRHLRKYMKEGGILVVRS 240

Query: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARK 281
           A+GGRAFLYPVV+VGDLVGFEILS+FHPTDDVVNSVILARK
Sbjct: 241 AKGGRAFLYPVVEVGDLVGFEILSVFHPTDDVVNSVILARK 281

[Close]

BLAST of MELO3C017475.jh1 vs. NCBI nr
Match: KAG6589715.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 450 bits (1158), Expect = 1.24e-157
Identity = 226/281 (80.43%), Postives = 255/281 (90.75%), Query Frame = 0


Query: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ  G E +IS  FL+ R+IDLH SI +LETL+PCK+VN LFTNLV LCILPCSIDV
Sbjct: 1   MAALQILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTNLVKLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120
           STL P+LQAIRESLI+LCG+AEGLLELEFST+L KIPKPLNNLTLFPYY NY+KLA+LE 
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYDNYIKLAHLEY 120

Query: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180
           KIL DNG+VNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYDVDS+AN+VA KIV SD 
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240
           ELEGRMKF + DIVEV EEL GYDCVFLAALVG+NKE+KVKI+ HLRKYMK+GG+L+VRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARK 281
           A+GGRAFLYPVV+V D+VGF+ILSIFHP+DDVVNSV+LARK
Sbjct: 241 ARGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARK 281

[Close]

BLAST of MELO3C017475.jh1 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 1.8e-76
Identity = 144/264 (54.55%), Postives = 192/264 (72.73%), Query Frame = 0


Query: 18  LVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDVSTLPPNLQAIRESLIIL 77
           +V+++ +L++ I +LE L P K VN LFT+LV+ C+ P  IDVS L   +Q IR  LI L
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNPIDVSKLCQKIQEIRSHLIKL 68

Query: 78  CGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLENKILNDNGIVNPKKVAFV 137
           CGQAEGLLE  FS +LS    PL +L +FPY+ NY+KL+ LE  IL  N    PKK+AF+
Sbjct: 69  CGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKIAFI 128

Query: 138 GSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDAELEGRMKFCSSDIVEVK 197
           GSGPLPLTS+++A +H+K T F NYD+D  AN +A  +V +D ++  RM F ++D+++V 
Sbjct: 129 GSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVMDVT 188

Query: 198 EELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRSAQGGRAFLYPVVKVGDL 257
             L  YD VFLAALVGM+KE+KVK+V HL KYM  G  L++RSA G RAFLYPV+   DL
Sbjct: 189 CALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDPRDL 248

Query: 258 VGFEILSIFHPTDDVVNSVILARK 282
            GFE+LS++HPTD+V+NSVI+ARK
Sbjct: 249 RGFEVLSVYHPTDEVINSVIIARK 272

[Close]

BLAST of MELO3C017475.jh1 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.2e-75
Identity = 144/268 (53.73%), Postives = 202/268 (75.37%), Query Frame = 0


Query: 18  LVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILP-CSIDVSTLPPNLQAIRESLII 77
           LV++I DL++ I KLETL+PC+ V+ LF  LV+ CI P  +IDV+ +  N+Q +R +LI 
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSNLIK 68

Query: 78  LCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYVKLANLENKIL--NDNGIVNPKK 137
           +CG+AEG LE  FS++L+     PL++L LFPYY NY+KL+ LE  +L  N NG V P+ 
Sbjct: 69  ICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV-PRT 128

Query: 138 VAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDAELEGRMKFCSSDI 197
           VAF+GSGPLPLTS+++A  H+K + F N+D+D  AN VA ++V SD +L  RM F + DI
Sbjct: 129 VAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDI 188

Query: 198 VEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRSAQGGRAFLYPVVK 257
           ++V E L G+D VFLAALVGM+K+EKVK+V HL K+M  G +L++RSA G RAFLYP+V+
Sbjct: 189 MDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVE 248

Query: 258 VGDLVGFEILSIFHPTDDVVNSVILARK 282
             DL GFE+LS++HPTD+V+NS++++RK
Sbjct: 249 PCDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of MELO3C017475.jh1 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 2.7e-72
Identity = 145/268 (54.10%), Postives = 195/268 (72.76%), Query Frame = 0


Query: 18  LVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILP-CSIDVSTLPPNLQAIRESLII 77
           LV  I DL++ I KLE+L+P + VN LF  LV+ CI P  +IDV+ +   +Q IR +LI 
Sbjct: 8   LVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLNLIK 67

Query: 78  LCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYVKLANLENKIL--NDNGIVNPKK 137
           +CG AEG LE  FS++L+     PL++L +FPYY NY+KL  LE  +L  N NG V PK 
Sbjct: 68  ICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFV-PKS 127

Query: 138 VAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDAELEGRMKFCSSDI 197
           VAF+GSGPLPLTSI++A  H+K T F N+D+D  AN +A  +V SD ++  RM F + DI
Sbjct: 128 VAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTVDI 187

Query: 198 VEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRSAQGGRAFLYPVVK 257
           ++V E L  +D VFLAALVGMNKEEKVK++ HL+K+M  G +L++RSA G RAFLYP+V+
Sbjct: 188 MDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIVE 247

Query: 258 VGDLVGFEILSIFHPTDDVVNSVILARK 282
             DL GFE+LSI+HPTDDV+NSV++++K
Sbjct: 248 PCDLQGFEVLSIYHPTDDVINSVVISKK 274

[Close]

BLAST of MELO3C017475.jh1 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.1e-68
Identity = 134/268 (50.00%), Postives = 188/268 (70.15%), Query Frame = 0


Query: 18  LVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCI-LPCSIDVSTLPPNLQAIRESLII 77
           +V +IIDL+  I KL++L+P K V+ LF  LV+ C+    +IDV+ +   ++ +R +LI 
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 78  LCGQAEGLLELEFSTLLSKIPK---PLNNLTLFPYYQNYVKLANLENKILNDNGIVNPKK 137
           LCG+AEG LE  FST+L  + +   PL++L +FPYY NY+KL  LE  +L+ +    P K
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 138 VAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDAELEGRMKFCSSDI 197
           +AFVGSGP+PLTSI++A  H+  T F N+D+DS AN +A  +V  D +L  RM F ++D+
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 198 VEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRSAQGGRAFLYPVVK 257
           +   E L  YD VFLAALVGM+KE KVK + HL K+M  G +L++RSA   RAFLYP+V 
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 258 VGDLVGFEILSIFHPTDDVVNSVILARK 282
             DL GF++L+I+HPTDDVVNSV++ARK
Sbjct: 248 SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of MELO3C017475.jh1 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.2e-67
Identity = 134/267 (50.19%), Postives = 186/267 (69.66%), Query Frame = 0


Query: 18  LVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCI-LPCSIDVSTL-PPNLQAIRESLI 77
           +V +I+DL+  I  LE+L+P K V+ LF  LV+ C+    +IDV+ +    ++ +R  LI
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVKDMRSHLI 67

Query: 78  ILCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYVKLANLENKILNDNGIVNPKKV 137
            LCG+AEG LE  FS +L      PLN+L +FPYY NY+KL  LE  +L+ +    P KV
Sbjct: 68  KLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTKV 127

Query: 138 AFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDAELEGRMKFCSSDIV 197
           AF+GSGP+PLTSI++A  H+  T F N+D+DS AN +A  +V  D++L  RM F ++D++
Sbjct: 128 AFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVL 187

Query: 198 EVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRSAQGGRAFLYPVVKV 257
             KE L  YD VFLAALVGM+KE KVK + HL K+M  G ++++RSA G RAFLYP+V  
Sbjct: 188 NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDS 247

Query: 258 GDLVGFEILSIFHPTDDVVNSVILARK 282
            DL GFE+L+I+HP+DDVVNSV++ARK
Sbjct: 248 CDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of MELO3C017475.jh1 vs. ExPASy TrEMBL
Match: A0A5A7UXS8 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G006240 PE=3 SV=1)

HSP 1 Score: 553 bits (1425), Expect = 1.20e-198
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0


Query: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120
           STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180
           KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240
           ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARKQTS 284
           AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARKQTS
Sbjct: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARKQTS 284

[Close]

BLAST of MELO3C017475.jh1 vs. ExPASy TrEMBL
Match: A0A5D3CIT2 (Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00680 PE=3 SV=1)

HSP 1 Score: 551 bits (1421), Expect = 4.90e-198
Identity = 283/284 (99.65%), Postives = 284/284 (100.00%), Query Frame = 0


Query: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120
           STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180
           KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240
           ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARKQTS 284
           AQGGRAFLYPVV+VGDLVGFEILSIFHPTDDVVNSVILARKQTS
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARKQTS 284

[Close]

BLAST of MELO3C017475.jh1 vs. ExPASy TrEMBL
Match: A0A1S3BXH0 (nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1)

HSP 1 Score: 551 bits (1421), Expect = 4.90e-198
Identity = 283/284 (99.65%), Postives = 284/284 (100.00%), Query Frame = 0


Query: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV
Sbjct: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120
           STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN
Sbjct: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120

Query: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180
           KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180

Query: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240
           ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS
Sbjct: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240

Query: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARKQTS 284
           AQGGRAFLYPVV+VGDLVGFEILSIFHPTDDVVNSVILARKQTS
Sbjct: 241 AQGGRAFLYPVVEVGDLVGFEILSIFHPTDDVVNSVILARKQTS 284

[Close]

BLAST of MELO3C017475.jh1 vs. ExPASy TrEMBL
Match: A0A0A0LUJ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423010 PE=3 SV=1)

HSP 1 Score: 522 bits (1344), Expect = 2.77e-186
Identity = 265/281 (94.31%), Postives = 274/281 (97.51%), Query Frame = 0


Query: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60
           MAALQ HG EAEISAAFLVDRIIDLH+SI KLETLRPCKKVNNLFTNLV LCILPCSIDV
Sbjct: 1   MAALQIHGTEAEISAAFLVDRIIDLHESISKLETLRPCKKVNNLFTNLVTLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120
           STLPPNLQ IRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNY+KLANLEN
Sbjct: 61  STLPPNLQVIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYIKLANLEN 120

Query: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180
           KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGT+FDNYDVDSVANDVARKIVGSD+
Sbjct: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTHFDNYDVDSVANDVARKIVGSDS 180

Query: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240
           +LEGRMKFCSSDIV+VKEELGGYDCVFLAALVGMNKEEKVKI+RHLRKYMKEGGILLVRS
Sbjct: 181 DLEGRMKFCSSDIVDVKEELGGYDCVFLAALVGMNKEEKVKIIRHLRKYMKEGGILLVRS 240

Query: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARK 281
           A+GGRAFLYPVV+V DLVGFEILSIFHPTDDVVNSVIL RK
Sbjct: 241 AKGGRAFLYPVVEVEDLVGFEILSIFHPTDDVVNSVILTRK 281

[Close]

BLAST of MELO3C017475.jh1 vs. ExPASy TrEMBL
Match: A0A6J1E292 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111430119 PE=3 SV=1)

HSP 1 Score: 446 bits (1146), Expect = 4.04e-156
Identity = 224/281 (79.72%), Postives = 254/281 (90.39%), Query Frame = 0


Query: 1   MAALQFHGMEAEISAAFLVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILPCSIDV 60
           MAAL   G E +IS  FL+ R+IDLH SI +LETL+PCK+VN LFT+LV LCILPCSIDV
Sbjct: 1   MAALHILGTETDISEEFLIARVIDLHGSISRLETLKPCKEVNGLFTSLVKLCILPCSIDV 60

Query: 61  STLPPNLQAIRESLIILCGQAEGLLELEFSTLLSKIPKPLNNLTLFPYYQNYVKLANLEN 120
           STL P+LQAIRESLI+LCG+AEGLLELEFST+L KIPKPLNNLTLFPYY NY+KLA+LE 
Sbjct: 61  STLAPHLQAIRESLILLCGRAEGLLELEFSTMLGKIPKPLNNLTLFPYYGNYIKLAHLEY 120

Query: 121 KILNDNGIVNPKKVAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDA 180
           KIL DNG+VNPKKVAFVGSGPLPLTSII+AMQHMK TYFDNYDVDS+AN+VA KIV SD 
Sbjct: 121 KILIDNGVVNPKKVAFVGSGPLPLTSIILAMQHMKNTYFDNYDVDSMANEVAGKIVASDG 180

Query: 181 ELEGRMKFCSSDIVEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRS 240
           ELEGRMKF + DIVEV EEL GYDCVFLAALVG+NKE+KVKI+ HLRKYMK+GG+L+VRS
Sbjct: 181 ELEGRMKFWTRDIVEVGEELEGYDCVFLAALVGLNKEDKVKILSHLRKYMKQGGVLVVRS 240

Query: 241 AQGGRAFLYPVVKVGDLVGFEILSIFHPTDDVVNSVILARK 281
           A+GGRAFLYPVV+V D+VGF+ILSIFHP+DDVVNSV+LARK
Sbjct: 241 AKGGRAFLYPVVEVADMVGFDILSIFHPSDDVVNSVLLARK 281

[Close]

BLAST of MELO3C017475.jh1 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 284.6 bits (727), Expect = 8.4e-77
Identity = 144/268 (53.73%), Postives = 202/268 (75.37%), Query Frame = 0


Query: 18  LVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILP-CSIDVSTLPPNLQAIRESLII 77
           LV++I DL++ I KLETL+PC+ V+ LF  LV+ CI P  +IDV+ +  N+Q +R +LI 
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRSNLIK 68

Query: 78  LCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYVKLANLENKIL--NDNGIVNPKK 137
           +CG+AEG LE  FS++L+     PL++L LFPYY NY+KL+ LE  +L  N NG V P+ 
Sbjct: 69  ICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV-PRT 128

Query: 138 VAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDAELEGRMKFCSSDI 197
           VAF+GSGPLPLTS+++A  H+K + F N+D+D  AN VA ++V SD +L  RM F + DI
Sbjct: 129 VAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDI 188

Query: 198 VEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRSAQGGRAFLYPVVK 257
           ++V E L G+D VFLAALVGM+K+EKVK+V HL K+M  G +L++RSA G RAFLYP+V+
Sbjct: 189 MDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVE 248

Query: 258 VGDLVGFEILSIFHPTDDVVNSVILARK 282
             DL GFE+LS++HPTD+V+NS++++RK
Sbjct: 249 PCDLEGFEVLSVYHPTDEVINSIVISRK 275

[Close]

BLAST of MELO3C017475.jh1 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 273.5 bits (698), Expect = 1.9e-73
Identity = 145/268 (54.10%), Postives = 195/268 (72.76%), Query Frame = 0


Query: 18  LVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCILP-CSIDVSTLPPNLQAIRESLII 77
           LV  I DL++ I KLE+L+P + VN LF  LV+ CI P  +IDV+ +   +Q IR +LI 
Sbjct: 8   LVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQEIRLNLIK 67

Query: 78  LCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYVKLANLENKIL--NDNGIVNPKK 137
           +CG AEG LE  FS++L+     PL++L +FPYY NY+KL  LE  +L  N NG V PK 
Sbjct: 68  ICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNGFV-PKS 127

Query: 138 VAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDAELEGRMKFCSSDI 197
           VAF+GSGPLPLTSI++A  H+K T F N+D+D  AN +A  +V SD ++  RM F + DI
Sbjct: 128 VAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTVDI 187

Query: 198 VEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRSAQGGRAFLYPVVK 257
           ++V E L  +D VFLAALVGMNKEEKVK++ HL+K+M  G +L++RSA G RAFLYP+V+
Sbjct: 188 MDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIVE 247

Query: 258 VGDLVGFEILSIFHPTDDVVNSVILARK 282
             DL GFE+LSI+HPTDDV+NSV++++K
Sbjct: 248 PCDLQGFEVLSIYHPTDDVINSVVISKK 274

[Close]

BLAST of MELO3C017475.jh1 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 261.5 bits (667), Expect = 7.7e-70
Identity = 134/268 (50.00%), Postives = 188/268 (70.15%), Query Frame = 0


Query: 18  LVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCI-LPCSIDVSTLPPNLQAIRESLII 77
           +V +IIDL+  I KL++L+P K V+ LF  LV+ C+    +IDV+ +   ++ +R +LI 
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 78  LCGQAEGLLELEFSTLLSKIPK---PLNNLTLFPYYQNYVKLANLENKILNDNGIVNPKK 137
           LCG+AEG LE  FST+L  + +   PL++L +FPYY NY+KL  LE  +L+ +    P K
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 138 VAFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDAELEGRMKFCSSDI 197
           +AFVGSGP+PLTSI++A  H+  T F N+D+DS AN +A  +V  D +L  RM F ++D+
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 198 VEVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRSAQGGRAFLYPVVK 257
           +   E L  YD VFLAALVGM+KE KVK + HL K+M  G +L++RSA   RAFLYP+V 
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 258 VGDLVGFEILSIFHPTDDVVNSVILARK 282
             DL GF++L+I+HPTDDVVNSV++ARK
Sbjct: 248 SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]

BLAST of MELO3C017475.jh1 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 258.1 bits (658), Expect = 8.5e-69
Identity = 134/267 (50.19%), Postives = 186/267 (69.66%), Query Frame = 0


Query: 18  LVDRIIDLHQSILKLETLRPCKKVNNLFTNLVNLCI-LPCSIDVSTL-PPNLQAIRESLI 77
           +V +I+DL+  I  LE+L+P K V+ LF  LV+ C+    +IDV+ +    ++ +R  LI
Sbjct: 8   VVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVKDMRSHLI 67

Query: 78  ILCGQAEGLLELEFSTLLSKI-PKPLNNLTLFPYYQNYVKLANLENKILNDNGIVNPKKV 137
            LCG+AEG LE  FS +L      PLN+L +FPYY NY+KL  LE  +L+ +    P KV
Sbjct: 68  KLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTHVPTKV 127

Query: 138 AFVGSGPLPLTSIIMAMQHMKGTYFDNYDVDSVANDVARKIVGSDAELEGRMKFCSSDIV 197
           AF+GSGP+PLTSI++A  H+  T F N+D+DS AN +A  +V  D++L  RM F ++D++
Sbjct: 128 AFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVL 187

Query: 198 EVKEELGGYDCVFLAALVGMNKEEKVKIVRHLRKYMKEGGILLVRSAQGGRAFLYPVVKV 257
             KE L  YD VFLAALVGM+KE KVK + HL K+M  G ++++RSA G RAFLYP+V  
Sbjct: 188 NAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDS 247

Query: 258 GDLVGFEILSIFHPTDDVVNSVILARK 282
            DL GFE+L+I+HP+DDVVNSV++ARK
Sbjct: 248 CDLKGFEVLTIYHPSDDVVNSVVIARK 274

[Close]

BLAST of MELO3C017475.jh1 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 56.2 bits (134), Expect = 4.9e-08
Identity = 30/56 (53.57%), Postives = 40/56 (71.43%), Query Frame = 0


Query: 230 MKEGGILLVRSAQGGRAFLYPVVKVGDLVGFEILSIFHP--TDDVVNSVILARKQT 284
           M  G +L++RSA+G R+FLY  V   DL GFE+L I+HP  +D  VNSV++ARK T
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVARKLT 56

The following BLAST results are available for this feature:

BLAST of MELO3C017475.jh1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0058291.1	2.48e-198	100.00	nicotianamine synthase-like [Cucumis melo var. makuwa]	[more]
XP_008453616.1	1.01e-197	99.65	PREDICTED: nicotianamine synthase-like [Cucumis melo] >TYK11847.1 nicotianamine ...	[more]
XP_004146360.2	5.72e-186	94.31	probable nicotianamine synthase 4 [Cucumis sativus] >KGN65443.1 hypothetical pro...	[more]
XP_038880344.1	8.69e-181	91.10	nicotianamine synthase-like [Benincasa hispida]	[more]
KAG6589715.1	1.24e-157	80.43	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MELO3C017475.jh1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGI7	1.8e-76	54.55	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
Q9C7X5	1.2e-75	53.73	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
O80483	2.7e-72	54.10	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FF79	1.1e-68	50.00	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9FKT9	1.2e-67	50.19	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of MELO3C017475.jh1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UXS8	1.20e-198	100.00	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A5D3CIT2	4.90e-198	99.65	Nicotianamine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3BXH0	4.90e-198	99.65	nicotianamine synthase-like OS=Cucumis melo OX=3656 GN=LOC103494275 PE=3 SV=1	[more]
A0A0A0LUJ7	2.77e-186	94.31	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423010 PE=3 SV=1	[more]
A0A6J1E292	4.04e-156	79.72	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111430119 PE=3 S...	[more]

BLAST of MELO3C017475.jh1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	8.4e-77	53.73	nicotianamine synthase 4	[more]
AT1G09240.1	1.9e-73	54.10	nicotianamine synthase 3	[more]
AT5G04950.1	7.7e-70	50.00	nicotianamine synthase 1	[more]
AT5G56080.1	8.5e-69	50.19	nicotianamine synthase 2	[more]
AT4G26483.1	4.9e-08	53.57	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 14..283 e-value: 3.5E-60 score: 205.4
None	No IPR available	PANTHER	PTHR32266:SF11	NICOTIANAMINE SYNTHASE	coord: 1..283
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 163..238 e-value: 0.00594631 score: 33.9427
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 15..283 e-value: 6.3E-103 score: 343.9
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 1..283
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 10..284 score: 48.707901
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 123..240

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C017475.jh1.t1	MELO3C017475.jh1.t1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity