MELO3C016752.jh1 (gene) Melon (Harukei-3) v1.41

Overview
NameMELO3C016752.jh1
Typegene
OrganismCucumis melo var. reticulatus cv. Harukei-3 (Melon (Harukei-3) v1.41)
Descriptionabscisic acid 8'-hydroxylase 4-like
Locationchr07: 2456596 .. 2459068 (+)
RNA-Seq ExpressionMELO3C016752.jh1
SyntenyMELO3C016752.jh1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonstart_codonCDSpolypeptidestop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAATATTTAAAAATAAAAAAGTTGTCATTTAATCAATTTCATATAGAGAAAGATTAAATGGGTGTAATTTTTCTATCTATTTTGATCTTTTTTCTCAGTCTATTATGGTACATTTCTTTAAAGAGAGACAAGAAGGCAGCCATTAGAGATGGAACTAAGCTCCCTCCTGGTTCTTTAGGATGGCCTTATATTGGAGAAACTCTTCAACTCTATTCTCAACACCCTGATTTTTTTTTCGCTGCGAAACAAAAAAGGTTCGAACCTTTTCTTTTTCGTTGTCAAAGTTACGAAATATCTTAATTGTTGAGCCAATGTTTTTTCTTTTTTGTTTCTTTTTAGGTATGGAGAGATTTTTAAGACCCATATACTTGGATGTCCTTGTGTGATGTTGGCTAGTCCTGAGGCAGCGAGATTTGTGTTAGTGACACAAGCTCATTTGTTTAAACCAACTTACCCCAAAAGCAAAGAGCTTTTAATTGGCCCATCTGCCCTGTTTTTTCACCAAGGTGATTACCATTGCCGTCTAAGGAAGCTGGTTCAGAACTCTCTTTCTTTGGATTCCATCCGTACCTTAGTCCCCGATATTGACTCCGTCGCCGCCTCCGCCCTCGACTCCTGGGCCACTTCCGAAGTCATTAACACCTTTCATGAAATGAAAAAGGTTCGTTAAGCTCCATCAGTCTAGAGACGTTTTTATAAGAAATGCATGTCAATTAACACTCAAAGGTTATCTGTGTTTATTAATTAGATATCATTTGAAGTTGGGATATTGACCATTTTTGGCCATTTGGAAGCTGCTTACAAAGATGACCTTAAGAAAAACTACAGCATATTGGATGCTGGCTACAATTCTTTTCCCACCAACATTCCTGGAACTCCATACAAAAAGGCACTCTCAGTAAGCCAAACTCAACAACAAAAATAAAATTAAAAAAAAAATAAGGCAAAAAGAAAAATTGTCTTATCTTATTTTATTATTATTTTAAAAGAATATGATATAAATACAAAAATTTTGGGGTTATTAACAGGCAAGAAAGAGGCTAAGTAAAATCCTAGGTGACATAATTCATGAGAGGGGAGAAAGAAGGCTGAGAGAAAAGGATTTATTAGGGAGCCTTCTCAATTCCATTGATGATGAATGTGAAGTTAAAATAAGTGATTCCCAAATAGCAGATAACATAATAGGAGTGCTTTTTGCTGCTCAAGACACCACTGCCAGTGTCATGACTTGGATTGTTAAGTACCTCCACGACCAACCCAAGCTTCTTCAATCCCTAAAGGTACGTATAGTCGGCCACCACAATATTTAGAGTGCACTATGCACTATTCACCTACATAATTTTGAGTTAAAAGTTCGATTTTTTTTTCTTTTCTTTCAGGCAGAGCAAGAGGAAATTCTAAAATTAAATAGTGAGAATAATCAAGGATTAAATTGGGTACAGACAAGGAACATGCCAATCACTTATAAGGTTAATTTTCATCATTATTATTAATATTAATCAATAAATGATTTTTAAGAGATTGATTTATGTGGATTATTTGTTAAAAACATAGATGGTATTGGAGAGCTTAAGGATGGCTAGCATTATATCATACACTTTTAGAGAGGCTGTAGCTGATGTGGAATACAAAGGTAAAAGGAAAAAAGAAAAACCGAAAATAAATATAATTTAATTTCTTTTCGTTTGAATATGATAATATAACAAATGAATTTTTGTGAAATGACGTGTAATAGGATACTTGATTCCAAAAGGTTGGAAAGTGATGCCTTTGTTCCGGAACATCCATCACAGTCCAGAATATTTTCCTGACCCTCACAATTTCGACCCTTCTAGATTTGAGGTAAAAAAAAACTAAAACTAAAACTAAGACTAAGACTAAAACTTTATAAAATTAAAATACTATCTTATTATTTTCATTAAAATGTGGGTTACTGTTCAGGTTGCACCAAAACCCTATACGTTTATGCCTTTTGGCAGTGGAGTTCATGCCTGCCCTGGAAATGAACTTGCCAAGTTGGAGATCTTGATCATGATTCATCATCTAGTCACCAAGTTCAGGTCAAAACGATTCGTTTACAGATTGAATATTTTGTTTTTTTTTATAAACTTTTTGAGAGTAATATCATATTTAAGTTATTTACTCACTTTTTGTTTATTTTTGCTGACTTTGGCAGGTGGGAATTGGTTGGGTCGCAAAACGGGGTTCAGCACAGTCCATTTCCCGTTCCCTTTCAAGGCCTTCCAGCCAGATTTTGGAAGATAGCCCATTAGAAAACCGTACACGGGAATATGAGCCCGCACTAAGTGGGCCGATTGCCGTCCAATCCAAATCCCAGCCAGCCCAGCCCAAGCCCAATACTTGGGCTATCGTTTCAAGAATCCATTTTCTTCTCTCCCCCTTGTCCATCTGTACTCAACGGTTTATATTAGTTTAAAATTTTAATTTGATAAATAATTAAAACTTTCAACT

mRNA sequence

CAAATATTTAAAAATAAAAAAGTTGTCATTTAATCAATTTCATATAGAGAAAGATTAAATGGGTGTAATTTTTCTATCTATTTTGATCTTTTTTCTCAGTCTATTATGGTACATTTCTTTAAAGAGAGACAAGAAGGCAGCCATTAGAGATGGAACTAAGCTCCCTCCTGGTTCTTTAGGATGGCCTTATATTGGAGAAACTCTTCAACTCTATTCTCAACACCCTGATTTTTTTTTCGCTGCGAAACAAAAAAGGTATGGAGAGATTTTTAAGACCCATATACTTGGATGTCCTTGTGTGATGTTGGCTAGTCCTGAGGCAGCGAGATTTGTGTTAGTGACACAAGCTCATTTGTTTAAACCAACTTACCCCAAAAGCAAAGAGCTTTTAATTGGCCCATCTGCCCTGTTTTTTCACCAAGGTGATTACCATTGCCGTCTAAGGAAGCTGGTTCAGAACTCTCTTTCTTTGGATTCCATCCGTACCTTAGTCCCCGATATTGACTCCGTCGCCGCCTCCGCCCTCGACTCCTGGGCCACTTCCGAAGTCATTAACACCTTTCATGAAATGAAAAAGATATCATTTGAAGTTGGGATATTGACCATTTTTGGCCATTTGGAAGCTGCTTACAAAGATGACCTTAAGAAAAACTACAGCATATTGGATGCTGGCTACAATTCTTTTCCCACCAACATTCCTGGAACTCCATACAAAAAGGCACTCTCAGCAAGAAAGAGGCTAAGTAAAATCCTAGGTGACATAATTCATGAGAGGGGAGAAAGAAGGCTGAGAGAAAAGGATTTATTAGGGAGCCTTCTCAATTCCATTGATGATGAATGTGAAGTTAAAATAAGTGATTCCCAAATAGCAGATAACATAATAGGAGTGCTTTTTGCTGCTCAAGACACCACTGCCAGTGTCATGACTTGGATTGTTAAGTACCTCCACGACCAACCCAAGCTTCTTCAATCCCTAAAGGCAGAGCAAGAGGAAATTCTAAAATTAAATAGTGAGAATAATCAAGGATTAAATTGGGTACAGACAAGGAACATGCCAATCACTTATAAGATGGTATTGGAGAGCTTAAGGATGGCTAGCATTATATCATACACTTTTAGAGAGGCTGTAGCTGATGTGGAATACAAAGGATACTTGATTCCAAAAGGTTGGAAAGTGATGCCTTTGTTCCGGAACATCCATCACAGTCCAGAATATTTTCCTGACCCTCACAATTTCGACCCTTCTAGATTTGAGGTTGCACCAAAACCCTATACGTTTATGCCTTTTGGCAGTGGAGTTCATGCCTGCCCTGGAAATGAACTTGCCAAGTTGGAGATCTTGATCATGATTCATCATCTAGTCACCAAGTTCAGGTCAAAACGATTCGTGGGAATTGGTTGGGTCGCAAAACGGGGTTCAGCACAGTCCATTTCCCGTTCCCTTTCAAGGCCTTCCAGCCAGATTTTGGAAGATAGCCCATTAGAAAACCGTACACGGGAATATGAGCCCGCACTAAGTGGGCCGATTGCCGTCCAATCCAAATCCCAGCCAGCCCAGCCCAAGCCCAATACTTGGGCTATCGTTTCAAGAATCCATTTTCTTCTCTCCCCCTTGTCCATCTGTACTCAACGGTTTATATTAGTTTAAAATTTTAATTTGATAAATAATTAAAACTTTCAACT

Coding sequence (CDS)

ATGGGTGTAATTTTTCTATCTATTTTGATCTTTTTTCTCAGTCTATTATGGTACATTTCTTTAAAGAGAGACAAGAAGGCAGCCATTAGAGATGGAACTAAGCTCCCTCCTGGTTCTTTAGGATGGCCTTATATTGGAGAAACTCTTCAACTCTATTCTCAACACCCTGATTTTTTTTTCGCTGCGAAACAAAAAAGGTATGGAGAGATTTTTAAGACCCATATACTTGGATGTCCTTGTGTGATGTTGGCTAGTCCTGAGGCAGCGAGATTTGTGTTAGTGACACAAGCTCATTTGTTTAAACCAACTTACCCCAAAAGCAAAGAGCTTTTAATTGGCCCATCTGCCCTGTTTTTTCACCAAGGTGATTACCATTGCCGTCTAAGGAAGCTGGTTCAGAACTCTCTTTCTTTGGATTCCATCCGTACCTTAGTCCCCGATATTGACTCCGTCGCCGCCTCCGCCCTCGACTCCTGGGCCACTTCCGAAGTCATTAACACCTTTCATGAAATGAAAAAGATATCATTTGAAGTTGGGATATTGACCATTTTTGGCCATTTGGAAGCTGCTTACAAAGATGACCTTAAGAAAAACTACAGCATATTGGATGCTGGCTACAATTCTTTTCCCACCAACATTCCTGGAACTCCATACAAAAAGGCACTCTCAGCAAGAAAGAGGCTAAGTAAAATCCTAGGTGACATAATTCATGAGAGGGGAGAAAGAAGGCTGAGAGAAAAGGATTTATTAGGGAGCCTTCTCAATTCCATTGATGATGAATGTGAAGTTAAAATAAGTGATTCCCAAATAGCAGATAACATAATAGGAGTGCTTTTTGCTGCTCAAGACACCACTGCCAGTGTCATGACTTGGATTGTTAAGTACCTCCACGACCAACCCAAGCTTCTTCAATCCCTAAAGGCAGAGCAAGAGGAAATTCTAAAATTAAATAGTGAGAATAATCAAGGATTAAATTGGGTACAGACAAGGAACATGCCAATCACTTATAAGATGGTATTGGAGAGCTTAAGGATGGCTAGCATTATATCATACACTTTTAGAGAGGCTGTAGCTGATGTGGAATACAAAGGATACTTGATTCCAAAAGGTTGGAAAGTGATGCCTTTGTTCCGGAACATCCATCACAGTCCAGAATATTTTCCTGACCCTCACAATTTCGACCCTTCTAGATTTGAGGTTGCACCAAAACCCTATACGTTTATGCCTTTTGGCAGTGGAGTTCATGCCTGCCCTGGAAATGAACTTGCCAAGTTGGAGATCTTGATCATGATTCATCATCTAGTCACCAAGTTCAGGTCAAAACGATTCGTGGGAATTGGTTGGGTCGCAAAACGGGGTTCAGCACAGTCCATTTCCCGTTCCCTTTCAAGGCCTTCCAGCCAGATTTTGGAAGATAGCCCATTAGAAAACCGTACACGGGAATATGAGCCCGCACTAAGTGGGCCGATTGCCGTCCAATCCAAATCCCAGCCAGCCCAGCCCAAGCCCAATACTTGGGCTATCGTTTCAAGAATCCATTTTCTTCTCTCCCCCTTGTCCATCTGTACTCAACGGTTTATATTAGTTTAA

Protein sequence

MGVIFLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFRSKRFVGIGWVAKRGSAQSISRSLSRPSSQILEDSPLENRTREYEPALSGPIAVQSKSQPAQPKPNTWAIVSRIHFLLSPLSICTQRFILV
Homology
BLAST of MELO3C016752.jh1 vs. NCBI nr
Match: XP_008452547.1 (PREDICTED: abscisic acid 8'-hydroxylase 4-like [Cucumis melo])

HSP 1 Score: 884 bits (2284), Expect = 0.0
Identity = 438/439 (99.77%), Postives = 438/439 (99.77%), Query Frame = 0

Query: 1   MGVIFLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 60
           MGVIFLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF
Sbjct: 1   MGVIFLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 120
           AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH
Sbjct: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 120

Query: 121 QGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGI 180

Query: 181 LTIFGHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERG 240
           LTIFGHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERG
Sbjct: 181 LTIFGHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERG 240

Query: 241 ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQP 300
           ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQP
Sbjct: 241 ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQP 300

Query: 301 KLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADV 360
           KLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADV
Sbjct: 301 KLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADV 360

Query: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGN 420
           EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKP TFMPFGSGVHACPGN
Sbjct: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGN 420

Query: 421 ELAKLEILIMIHHLVTKFR 439
           ELAKLEILIMIHHLVTKFR
Sbjct: 421 ELAKLEILIMIHHLVTKFR 439

BLAST of MELO3C016752.jh1 vs. NCBI nr
Match: XP_004141255.1 (abscisic acid 8'-hydroxylase CYP707A1 [Cucumis sativus] >KGN55192.1 hypothetical protein Csa_011955 [Cucumis sativus])

HSP 1 Score: 865 bits (2236), Expect = 1.31e-314
Identity = 426/439 (97.04%), Postives = 434/439 (98.86%), Query Frame = 0

Query: 1   MGVIFLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 60
           MGV+FLSILIFFL+LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF
Sbjct: 1   MGVVFLSILIFFLTLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 120
           AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH
Sbjct: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 120

Query: 121 QGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQ+SLSLDSIRTLVPDIDSVAASALDSWATS VINTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQSSLSLDSIRTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGI 180

Query: 181 LTIFGHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERG 240
           LTIFGHLEAAYKDDLKKNYSIL+AGYNSFPTNIPGTPYKKALSARKRL+KILGDIIHERG
Sbjct: 181 LTIFGHLEAAYKDDLKKNYSILEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERG 240

Query: 241 ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQP 300
           ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIG LFAAQDTTASVMTWIVKYLHD+P
Sbjct: 241 ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRP 300

Query: 301 KLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADV 360
           KL QS+KAEQEEILKLN ENNQGLNWVQTRNMPITYKMVLESLRMASIIS+TFREAVADV
Sbjct: 301 KLRQSIKAEQEEILKLNCENNQGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADV 360

Query: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGN 420
           EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKP TFMPFGSGVHACPGN
Sbjct: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGN 420

Query: 421 ELAKLEILIMIHHLVTKFR 439
           ELAKLEILIMIHHLVTKFR
Sbjct: 421 ELAKLEILIMIHHLVTKFR 439

BLAST of MELO3C016752.jh1 vs. NCBI nr
Match: KAA0064383.1 (abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 862 bits (2227), Expect = 5.34e-313
Identity = 424/425 (99.76%), Postives = 424/425 (99.76%), Query Frame = 0

Query: 15  LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTH 74
           LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTH
Sbjct: 31  LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTH 90

Query: 75  ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQN 134
           ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQN
Sbjct: 91  ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQN 150

Query: 135 SLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDD 194
           SLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDD
Sbjct: 151 SLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDD 210

Query: 195 LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLL 254
           LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLL
Sbjct: 211 LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLL 270

Query: 255 NSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEIL 314
           NSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEIL
Sbjct: 271 NSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEIL 330

Query: 315 KLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVM 374
           KLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVM
Sbjct: 331 KLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVM 390

Query: 375 PLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGNELAKLEILIMIHHL 434
           PLFRNIHHSPEYFPDPHNFDPSRFEVAPKP TFMPFGSGVHACPGNELAKLEILIMIHHL
Sbjct: 391 PLFRNIHHSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHL 450

Query: 435 VTKFR 439
           VTKFR
Sbjct: 451 VTKFR 455

BLAST of MELO3C016752.jh1 vs. NCBI nr
Match: TYK20204.1 (abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 831 bits (2146), Expect = 7.62e-301
Identity = 413/425 (97.18%), Postives = 413/425 (97.18%), Query Frame = 0

Query: 15  LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTH 74
           LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTH
Sbjct: 31  LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTH 90

Query: 75  ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQN 134
           ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQN
Sbjct: 91  ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQN 150

Query: 135 SLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDD 194
           SLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDD
Sbjct: 151 SLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDD 210

Query: 195 LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLL 254
           LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLL
Sbjct: 211 LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLL 270

Query: 255 NSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEIL 314
           NSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEIL
Sbjct: 271 NSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEIL 330

Query: 315 KLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVM 374
           KLNSENNQGLNW           MVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVM
Sbjct: 331 KLNSENNQGLNW-----------MVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVM 390

Query: 375 PLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGNELAKLEILIMIHHL 434
           PLFRNIHHSPEYFPDPHNFDPSRFEVAPKP TFMPFGSGVHACPGNELAKLEILIMIHHL
Sbjct: 391 PLFRNIHHSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHL 444

Query: 435 VTKFR 439
           VTKFR
Sbjct: 451 VTKFR 444

BLAST of MELO3C016752.jh1 vs. NCBI nr
Match: XP_038897319.1 (abscisic acid 8'-hydroxylase CYP707A1-like [Benincasa hispida])

HSP 1 Score: 826 bits (2133), Expect = 6.02e-299
Identity = 407/439 (92.71%), Postives = 425/439 (96.81%), Query Frame = 0

Query: 1   MGVIFLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 60
           MGVIFLS+L+FFL+LLWYISLKR+KKAAIR GTKLPPGSLGWPYIGETLQLYSQ P+FFF
Sbjct: 1   MGVIFLSVLVFFLTLLWYISLKRNKKAAIRYGTKLPPGSLGWPYIGETLQLYSQDPEFFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 120
           A KQKRYGEIFKTHILGCPCVMLASPEAARFVL+TQAHLFKPTYPKSKELLIGPSALFFH
Sbjct: 61  ATKQKRYGEIFKTHILGCPCVMLASPEAARFVLMTQAHLFKPTYPKSKELLIGPSALFFH 120

Query: 121 QGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQNSLSLD+IRTL+PDI+SVAASALDSWAT+ +INTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQNSLSLDAIRTLIPDINSVAASALDSWATAGLINTFHEMKKISFEVGI 180

Query: 181 LTIFGHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERG 240
           LTIFGHLEAAYK+DLK NYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHER 
Sbjct: 181 LTIFGHLEAAYKEDLKINYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240

Query: 241 ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQP 300
           E RL EKDLLGSLLNS+DDE EVKISD+QIADNIIGVLFAAQDTTASVMTWIVKYLHD+P
Sbjct: 241 EERLGEKDLLGSLLNSLDDEGEVKISDAQIADNIIGVLFAAQDTTASVMTWIVKYLHDRP 300

Query: 301 KLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADV 360
           KLLQS+KAEQEEILKLN EN Q LNWVQTRNMPI+YKMVLESLRMASIIS+TFREAVADV
Sbjct: 301 KLLQSIKAEQEEILKLNCENGQRLNWVQTRNMPISYKMVLESLRMASIISFTFREAVADV 360

Query: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGN 420
           EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFD SRFEVAPKP TFMPFGSGVHACPGN
Sbjct: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDSSRFEVAPKPNTFMPFGSGVHACPGN 420

Query: 421 ELAKLEILIMIHHLVTKFR 439
           ELAKLEILIMIHHLVTKFR
Sbjct: 421 ELAKLEILIMIHHLVTKFR 439

BLAST of MELO3C016752.jh1 vs. ExPASy Swiss-Prot
Match: A9QNE7 (Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A1 PE=2 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 2.5e-175
Identity = 303/438 (69.18%), Postives = 356/438 (81.28%), Query Frame = 0

Query: 4   IFLSILIFFLSLLWYISL--KRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFA 63
           IFL I +F L  L Y     K +  ++ ++  KLPPGS+GWPYIGETLQLYSQ P+ FF 
Sbjct: 7   IFLYISMFVLGYLSYYFCFGKNNNSSSKKNAYKLPPGSMGWPYIGETLQLYSQDPNAFFI 66

Query: 64  AKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQ 123
            +Q+R+GEIFKT ILGCPCVMLASPEAARFVLV QA+LFKPTYPKSKE LIG SA+FFHQ
Sbjct: 67  NRQRRFGEIFKTKILGCPCVMLASPEAARFVLVNQANLFKPTYPKSKENLIGQSAIFFHQ 126

Query: 124 GDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGIL 183
           GDYH  LRKLVQ  L+ +SIR  +P I+ ++ SAL+SW    V+NT+HEMKK SFEVGIL
Sbjct: 127 GDYHNHLRKLVQAPLNPESIRNQIPYIEELSISALNSWVGGHVVNTYHEMKKFSFEVGIL 186

Query: 184 TIFGHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGE 243
            IFGHL+   K++LKKNYSI+D GYNSFP N+PGT Y+KAL ARK+L KIL +II E  E
Sbjct: 187 AIFGHLDGHVKEELKKNYSIVDKGYNSFPINLPGTLYRKALQARKKLGKILSEIIREMKE 246

Query: 244 RRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPK 303
           ++  EK LL   LN+ +++  + +++ QIADNIIGVLFAAQDTTASV+TWI+KYLHD PK
Sbjct: 247 KKTLEKGLLSCFLNAKEEKGFLVLNEDQIADNIIGVLFAAQDTTASVLTWIIKYLHDNPK 306

Query: 304 LLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVE 363
           LL+ +KAEQ+ I + N + N GL W QTR MPIT ++VLE+LRMASIIS+ FREAVADVE
Sbjct: 307 LLECVKAEQKVIWQSNEQENHGLTWTQTRKMPITSRVVLETLRMASIISFAFREAVADVE 366

Query: 364 YKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGNE 423
           YKGYLIPKGWKVMPLFRNIHH+PE+FPDP  FDPSRFE APKP TFMPFGSGVHACPGNE
Sbjct: 367 YKGYLIPKGWKVMPLFRNIHHNPEFFPDPQKFDPSRFENAPKPNTFMPFGSGVHACPGNE 426

Query: 424 LAKLEILIMIHHLVTKFR 440
           LAKLEILIM HHLVTKFR
Sbjct: 427 LAKLEILIMTHHLVTKFR 444

BLAST of MELO3C016752.jh1 vs. ExPASy Swiss-Prot
Match: Q9LJK2 (Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 SV=2)

HSP 1 Score: 595.1 bits (1533), Expect = 7.7e-169
Identity = 292/435 (67.13%), Postives = 352/435 (80.92%), Query Frame = 0

Query: 5   FLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQ 64
           FL + I  L LL    +   KK   R   KLPPGS+GWPY+GETLQLYSQ+P+ FF +KQ
Sbjct: 6   FLVVPILILCLLLVRVIVSKKKKNSRG--KLPPGSMGWPYLGETLQLYSQNPNVFFTSKQ 65

Query: 65  KRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDY 124
           KRYGEIFKT ILG PCVMLASPEAARFVLVT AH+FKPTYP+SKE LIGPSALFFHQGDY
Sbjct: 66  KRYGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDY 125

Query: 125 HCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIF 184
           H  +RKLVQ+S   ++IR L+PDI+ +A S+L SWA   +++T+ EMKK +F+VGIL IF
Sbjct: 126 HSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAIF 185

Query: 185 GHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRL 244
           GHLE++YK+ LK NY+I+D GYNSFP ++PGT Y KAL ARK+L  I+ +II ER E+R 
Sbjct: 186 GHLESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRA 245

Query: 245 REKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQ 304
            + D LG LLN  +++  V ++  QIADNIIGVLFAAQDTTAS +TWI+KYLHD  KLL+
Sbjct: 246 LQTDFLGHLLNFKNEKGRV-LTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLE 305

Query: 305 SLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKG 364
           ++KAEQ+ I + NS   + L W QTRNMP+T+K+++ESLRMASIIS+TFREAV DVEYKG
Sbjct: 306 AVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKG 365

Query: 365 YLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGNELAK 424
           YLIPKGWKVMPLFRNIHH+P+YF +P  FDPSRFEV PKP TFMPFGSGVHACPGNELAK
Sbjct: 366 YLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNELAK 425

Query: 425 LEILIMIHHLVTKFR 440
           L+ILI +HHLV+ FR
Sbjct: 426 LQILIFLHHLVSNFR 437

BLAST of MELO3C016752.jh1 vs. ExPASy Swiss-Prot
Match: Q0J185 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A7 PE=2 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 8.8e-165
Identity = 287/440 (65.23%), Postives = 346/440 (78.64%), Query Frame = 0

Query: 3   VIFLSILIFFLSLLWYISLKRDKKAAI--RDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 62
           VI   I + F+  + Y S +R K          +LPPGS+GWPYIGETLQLYSQ P+ FF
Sbjct: 10  VISFFISLAFMCYVHYTSRQRRKLHGYGHEKAVRLPPGSMGWPYIGETLQLYSQDPNVFF 69

Query: 63  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 122
           A+KQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYP+SKE +IGPSALFFH
Sbjct: 70  ASKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPRSKERMIGPSALFFH 129

Query: 123 QGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGI 182
           QGDYH RLRKLVQ  L  D++R LVPD+++   S L SW    V +TFH MK++SF+VGI
Sbjct: 130 QGDYHLRLRKLVQGPLGPDALRALVPDVEAAVRSTLASW-DGNVSSTFHAMKRLSFDVGI 189

Query: 183 LTIF-GHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHER 242
           +TIF G L+   K +L++NY+I++ GYNSFP + PGT Y KA+ AR+RL  +L DI+ ER
Sbjct: 190 VTIFGGRLDERRKAELRQNYAIVEKGYNSFPNSFPGTLYYKAIQARRRLHGVLSDIMRER 249

Query: 243 GERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQ 302
             R     DLLG L+ S   +    ++D Q+ADNIIGVLFAAQDTTASV+TWIVKYLHD 
Sbjct: 250 RARGEPGSDLLGCLMQSRAGDDGALLTDEQVADNIIGVLFAAQDTTASVLTWIVKYLHDH 309

Query: 303 PKLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVAD 362
           PKLL++++AEQ  I   N      L W QTR+M +T+K++LESLRMASIIS+TFREAVAD
Sbjct: 310 PKLLEAVRAEQAAIRAANDGGRLPLTWAQTRSMALTHKVILESLRMASIISFTFREAVAD 369

Query: 363 VEYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPG 422
           VEYKG+LIPKGWKVMPLFRNIHH+P+YF DP  FDPSRF+V+P+P TFMPFG+GVHACPG
Sbjct: 370 VEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQKFDPSRFKVSPRPNTFMPFGNGVHACPG 429

Query: 423 NELAKLEILIMIHHLVTKFR 440
           NELAKLE+L++IHHLVT +R
Sbjct: 430 NELAKLEMLVLIHHLVTGYR 448

BLAST of MELO3C016752.jh1 vs. ExPASy Swiss-Prot
Match: A2Z212 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A7 PE=3 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 5.7e-164
Identity = 286/440 (65.00%), Postives = 346/440 (78.64%), Query Frame = 0

Query: 3   VIFLSILIFFLSLLWYISLKRDKKAAI--RDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 62
           VI   I + F+  + Y S +R K          +LPPGS+GWPYIGETLQLYSQ P+ FF
Sbjct: 10  VISFFISLAFMCYVHYTSRQRRKLHGYGHEKAVRLPPGSMGWPYIGETLQLYSQDPNVFF 69

Query: 63  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 122
           A+KQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYP+SKE +IGPSALFF+
Sbjct: 70  ASKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPRSKERMIGPSALFFN 129

Query: 123 QGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGI 182
           QGDYH RLRKLVQ  L  D++R LVPD+++   S L SW    V +TFH MK++SF+VGI
Sbjct: 130 QGDYHLRLRKLVQGPLGPDALRALVPDVEAAVRSTLASW-DGNVSSTFHAMKRLSFDVGI 189

Query: 183 LTIF-GHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHER 242
           +TIF G L+   K +L++NY+I++ GYNSFP + PGT Y KA+ AR+RL  +L DI+ ER
Sbjct: 190 VTIFGGRLDERRKAELRQNYAIVEKGYNSFPNSFPGTLYYKAIQARRRLHGVLSDIMRER 249

Query: 243 GERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQ 302
             R     DLLG L+ S   +    ++D Q+ADNIIGVLFAAQDTTASV+TWIVKYLHD 
Sbjct: 250 RARGEPGSDLLGCLMQSRAGDDGALLTDEQVADNIIGVLFAAQDTTASVLTWIVKYLHDH 309

Query: 303 PKLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVAD 362
           PKLL++++AEQ  I   N      L W QTR+M +T+K++LESLRMASIIS+TFREAVAD
Sbjct: 310 PKLLEAVRAEQAAIRAANDGGRLPLTWAQTRSMALTHKVILESLRMASIISFTFREAVAD 369

Query: 363 VEYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPG 422
           VEYKG+LIPKGWKVMPLFRNIHH+P+YF DP  FDPSRF+V+P+P TFMPFG+GVHACPG
Sbjct: 370 VEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQKFDPSRFKVSPRPNTFMPFGNGVHACPG 429

Query: 423 NELAKLEILIMIHHLVTKFR 440
           NELAKLE+L++IHHLVT +R
Sbjct: 430 NELAKLEMLVLIHHLVTGYR 448

BLAST of MELO3C016752.jh1 vs. ExPASy Swiss-Prot
Match: Q09J78 (Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A6 PE=2 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 6.9e-162
Identity = 271/410 (66.10%), Postives = 335/410 (81.71%), Query Frame = 0

Query: 34  KLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTHILGCPCVMLASPEAARFVL 93
           +LPPGS+GWPY+GETLQLYSQ P+ FFA+KQKRYGEIFKT++LGCPCVMLASPEAARFVL
Sbjct: 50  RLPPGSMGWPYVGETLQLYSQDPNVFFASKQKRYGEIFKTNLLGCPCVMLASPEAARFVL 109

Query: 94  VTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAA 153
           V+QA LFKPTYP SKE +IGPSALFFHQG+YH RLR+LVQ +L+ DS+R LVPD+D+  A
Sbjct: 110 VSQARLFKPTYPPSKERMIGPSALFFHQGEYHLRLRRLVQAALAPDSLRALVPDVDAAVA 169

Query: 154 SALDSWATSEVINTFHEMKKISFEVGILTIF-GHLEAAYKDDLKKNYSILDAGYNSFPTN 213
           + L +W+   V +TFH MKK+SF+VG++TIF G L   ++++L+ NYS+++ GYN FP  
Sbjct: 170 ATLAAWSGGHVASTFHAMKKLSFDVGVVTIFGGRLGRRHREELRTNYSVVERGYNCFPNR 229

Query: 214 IPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLLNSIDD---ECEVKISDSQ 273
            PGT Y KA+ ARKRL  IL +I+ ER  R     DLLG L+ S DD        ++D Q
Sbjct: 230 FPGTLYHKAIQARKRLRAILSEIVAERRARGGGGDDLLGGLMRSRDDGTAGAVALLTDDQ 289

Query: 274 IADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEILKLNSENNQGLNWVQT 333
           IADN++GVLFAAQDTTASV+TWI+KYLHD PKLL+++KAEQ  I   N    + L W QT
Sbjct: 290 IADNVVGVLFAAQDTTASVLTWILKYLHDSPKLLEAVKAEQMAIYVANEGGKRPLTWTQT 349

Query: 334 RNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVMPLFRNIHHSPEYFPD 393
           R+M +T++++LESLRMASIIS+TFREAVADVEYKG+LIPKGWKVMPLFRNIHH+P+YF D
Sbjct: 350 RSMTLTHQVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQD 409

Query: 394 PHNFDPSRFEVAPKPYTFMPFGSGVHACPGNELAKLEILIMIHHLVTKFR 440
           P  FDPSRF+VAP+P TF+PFGSGVHACPGNELAKLE+L+++H LVT +R
Sbjct: 410 PQKFDPSRFKVAPRPSTFLPFGSGVHACPGNELAKLEMLVLVHRLVTAYR 459

BLAST of MELO3C016752.jh1 vs. ExPASy TrEMBL
Match: A0A1S3BTH6 (abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo OX=3656 GN=LOC103493539 PE=3 SV=1)

HSP 1 Score: 884 bits (2284), Expect = 0.0
Identity = 438/439 (99.77%), Postives = 438/439 (99.77%), Query Frame = 0

Query: 1   MGVIFLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 60
           MGVIFLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF
Sbjct: 1   MGVIFLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 120
           AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH
Sbjct: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 120

Query: 121 QGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGI 180

Query: 181 LTIFGHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERG 240
           LTIFGHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERG
Sbjct: 181 LTIFGHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERG 240

Query: 241 ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQP 300
           ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQP
Sbjct: 241 ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQP 300

Query: 301 KLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADV 360
           KLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADV
Sbjct: 301 KLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADV 360

Query: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGN 420
           EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKP TFMPFGSGVHACPGN
Sbjct: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGN 420

Query: 421 ELAKLEILIMIHHLVTKFR 439
           ELAKLEILIMIHHLVTKFR
Sbjct: 421 ELAKLEILIMIHHLVTKFR 439

BLAST of MELO3C016752.jh1 vs. ExPASy TrEMBL
Match: A0A0A0L3K1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639800 PE=3 SV=1)

HSP 1 Score: 865 bits (2236), Expect = 6.33e-315
Identity = 426/439 (97.04%), Postives = 434/439 (98.86%), Query Frame = 0

Query: 1   MGVIFLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 60
           MGV+FLSILIFFL+LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF
Sbjct: 1   MGVVFLSILIFFLTLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 120
           AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH
Sbjct: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 120

Query: 121 QGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQ+SLSLDSIRTLVPDIDSVAASALDSWATS VINTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQSSLSLDSIRTLVPDIDSVAASALDSWATSGVINTFHEMKKISFEVGI 180

Query: 181 LTIFGHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERG 240
           LTIFGHLEAAYKDDLKKNYSIL+AGYNSFPTNIPGTPYKKALSARKRL+KILGDIIHERG
Sbjct: 181 LTIFGHLEAAYKDDLKKNYSILEAGYNSFPTNIPGTPYKKALSARKRLNKILGDIIHERG 240

Query: 241 ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQP 300
           ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIG LFAAQDTTASVMTWIVKYLHD+P
Sbjct: 241 ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGALFAAQDTTASVMTWIVKYLHDRP 300

Query: 301 KLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADV 360
           KL QS+KAEQEEILKLN ENNQGLNWVQTRNMPITYKMVLESLRMASIIS+TFREAVADV
Sbjct: 301 KLRQSIKAEQEEILKLNCENNQGLNWVQTRNMPITYKMVLESLRMASIISFTFREAVADV 360

Query: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGN 420
           EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKP TFMPFGSGVHACPGN
Sbjct: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGN 420

Query: 421 ELAKLEILIMIHHLVTKFR 439
           ELAKLEILIMIHHLVTKFR
Sbjct: 421 ELAKLEILIMIHHLVTKFR 439

BLAST of MELO3C016752.jh1 vs. ExPASy TrEMBL
Match: A0A5A7V8C1 (Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00840 PE=3 SV=1)

HSP 1 Score: 862 bits (2227), Expect = 2.59e-313
Identity = 424/425 (99.76%), Postives = 424/425 (99.76%), Query Frame = 0

Query: 15  LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTH 74
           LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTH
Sbjct: 31  LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTH 90

Query: 75  ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQN 134
           ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQN
Sbjct: 91  ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQN 150

Query: 135 SLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDD 194
           SLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDD
Sbjct: 151 SLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDD 210

Query: 195 LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLL 254
           LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLL
Sbjct: 211 LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLL 270

Query: 255 NSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEIL 314
           NSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEIL
Sbjct: 271 NSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEIL 330

Query: 315 KLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVM 374
           KLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVM
Sbjct: 331 KLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVM 390

Query: 375 PLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGNELAKLEILIMIHHL 434
           PLFRNIHHSPEYFPDPHNFDPSRFEVAPKP TFMPFGSGVHACPGNELAKLEILIMIHHL
Sbjct: 391 PLFRNIHHSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHL 450

Query: 435 VTKFR 439
           VTKFR
Sbjct: 451 VTKFR 455

BLAST of MELO3C016752.jh1 vs. ExPASy TrEMBL
Match: A0A5D3D9K5 (Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003410 PE=3 SV=1)

HSP 1 Score: 831 bits (2146), Expect = 3.69e-301
Identity = 413/425 (97.18%), Postives = 413/425 (97.18%), Query Frame = 0

Query: 15  LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTH 74
           LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTH
Sbjct: 31  LLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKRYGEIFKTH 90

Query: 75  ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQN 134
           ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQN
Sbjct: 91  ILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHCRLRKLVQN 150

Query: 135 SLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDD 194
           SLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDD
Sbjct: 151 SLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGHLEAAYKDD 210

Query: 195 LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLL 254
           LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLL
Sbjct: 211 LKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLREKDLLGSLL 270

Query: 255 NSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEIL 314
           NSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEIL
Sbjct: 271 NSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSLKAEQEEIL 330

Query: 315 KLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVM 374
           KLNSENNQGLNW           MVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVM
Sbjct: 331 KLNSENNQGLNW-----------MVLESLRMASIISYTFREAVADVEYKGYLIPKGWKVM 390

Query: 375 PLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGNELAKLEILIMIHHL 434
           PLFRNIHHSPEYFPDPHNFDPSRFEVAPKP TFMPFGSGVHACPGNELAKLEILIMIHHL
Sbjct: 391 PLFRNIHHSPEYFPDPHNFDPSRFEVAPKPNTFMPFGSGVHACPGNELAKLEILIMIHHL 444

Query: 435 VTKFR 439
           VTKFR
Sbjct: 451 VTKFR 444

BLAST of MELO3C016752.jh1 vs. ExPASy TrEMBL
Match: A0A6J1IYR9 (abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111481123 PE=3 SV=1)

HSP 1 Score: 764 bits (1972), Expect = 1.37e-274
Identity = 377/439 (85.88%), Postives = 404/439 (92.03%), Query Frame = 0

Query: 1   MGVIFLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFF 60
           MGVIF S+L+FFL +L YI L R K+A IRDG KLPPGSLGWPYIGETLQLYSQ PD FF
Sbjct: 1   MGVIFASLLLFFLIVLSYIYLNRTKRAGIRDGAKLPPGSLGWPYIGETLQLYSQDPDLFF 60

Query: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFH 120
           AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKE LIGPSALF+H
Sbjct: 61  AAKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKERLIGPSALFYH 120

Query: 121 QGDYHCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGI 180
           QGDYHCRLRKLVQNSLSLD IR+LVPDIDSV  SAL SW  +++INTFHEMKKISFEVGI
Sbjct: 121 QGDYHCRLRKLVQNSLSLDVIRSLVPDIDSVTTSALHSWTGTQLINTFHEMKKISFEVGI 180

Query: 181 LTIFGHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERG 240
           L IFGHLEAAYK+DLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHER 
Sbjct: 181 LAIFGHLEAAYKEDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERR 240

Query: 241 ERRLREKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQP 300
           E+++ +KDLLG  LNSID+E E  +SD+QI DNIIGVLFAAQDTTASVMTW+VKYL+DQP
Sbjct: 241 EKKVGKKDLLGWFLNSIDEEGEA-MSDAQIEDNIIGVLFAAQDTTASVMTWVVKYLYDQP 300

Query: 301 KLLQSLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADV 360
           KLLQ+LK EQEEIL+ N + NQ LNWVQTRNMP++YK+VLESLRMASIIS+TFREAVADV
Sbjct: 301 KLLQTLKKEQEEILEFNCQKNQQLNWVQTRNMPMSYKVVLESLRMASIISFTFREAVADV 360

Query: 361 EYKGYLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGN 420
           EYKGYLIPKGWKVMPLFRNIH SPEYF DPH FDPSRFEVAPKP TFMPFGSGVHACPGN
Sbjct: 361 EYKGYLIPKGWKVMPLFRNIHRSPEYFADPHKFDPSRFEVAPKPNTFMPFGSGVHACPGN 420

Query: 421 ELAKLEILIMIHHLVTKFR 439
           ELAKLEILI+IHHLVTKFR
Sbjct: 421 ELAKLEILILIHHLVTKFR 438

BLAST of MELO3C016752.jh1 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 595.1 bits (1533), Expect = 5.4e-170
Identity = 292/435 (67.13%), Postives = 352/435 (80.92%), Query Frame = 0

Query: 5   FLSILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQ 64
           FL + I  L LL    +   KK   R   KLPPGS+GWPY+GETLQLYSQ+P+ FF +KQ
Sbjct: 6   FLVVPILILCLLLVRVIVSKKKKNSRG--KLPPGSMGWPYLGETLQLYSQNPNVFFTSKQ 65

Query: 65  KRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDY 124
           KRYGEIFKT ILG PCVMLASPEAARFVLVT AH+FKPTYP+SKE LIGPSALFFHQGDY
Sbjct: 66  KRYGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDY 125

Query: 125 HCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIF 184
           H  +RKLVQ+S   ++IR L+PDI+ +A S+L SWA   +++T+ EMKK +F+VGIL IF
Sbjct: 126 HSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQEMKKFAFDVGILAIF 185

Query: 185 GHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRL 244
           GHLE++YK+ LK NY+I+D GYNSFP ++PGT Y KAL ARK+L  I+ +II ER E+R 
Sbjct: 186 GHLESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRA 245

Query: 245 REKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQ 304
            + D LG LLN  +++  V ++  QIADNIIGVLFAAQDTTAS +TWI+KYLHD  KLL+
Sbjct: 246 LQTDFLGHLLNFKNEKGRV-LTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLE 305

Query: 305 SLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKG 364
           ++KAEQ+ I + NS   + L W QTRNMP+T+K+++ESLRMASIIS+TFREAV DVEYKG
Sbjct: 306 AVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKG 365

Query: 365 YLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGNELAK 424
           YLIPKGWKVMPLFRNIHH+P+YF +P  FDPSRFEV PKP TFMPFGSGVHACPGNELAK
Sbjct: 366 YLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVHACPGNELAK 425

Query: 425 LEILIMIHHLVTKFR 440
           L+ILI +HHLV+ FR
Sbjct: 426 LQILIFLHHLVSNFR 437

BLAST of MELO3C016752.jh1 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 516.5 bits (1329), Expect = 2.5e-146
Identity = 250/433 (57.74%), Postives = 328/433 (75.75%), Query Frame = 0

Query: 7   SILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKR 66
           S+ ++FL  L  IS +R   + +     LPPG++GWPY+GET QLYSQ P+ FF +KQKR
Sbjct: 14  SLFLYFLRCL--ISQRRFGSSKL----PLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKR 73

Query: 67  YGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHC 126
           YG +FKTH+LGCPCVM++SPEAA+FVLVT++HLFKPT+P SKE ++G  A+FFHQGDYH 
Sbjct: 74  YGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHA 133

Query: 127 RLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGH 186
           +LRKLV  +   +SIR +VPDI+S+A  +L SW    +INT+ EMK  +F V +L+IFG 
Sbjct: 134 KLRKLVLRAFMPESIRNMVPDIESIAQDSLRSW-EGTMINTYQEMKTYTFNVALLSIFGK 193

Query: 187 LEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLRE 246
            E  Y++DLK+ Y IL+ GYNS P N+PGT + K++ ARK LS+IL  I+ ER +     
Sbjct: 194 DEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSH 253

Query: 247 KDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSL 306
            DLLGS +   ++     ++D QIADNIIGV+FAA+DTTASVM+WI+KYL + P +L+++
Sbjct: 254 NDLLGSFMGDKEE-----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAV 313

Query: 307 KAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYL 366
             EQ  I K + E  + L W  T+ MP+T +++ E+LR+ASI+S+TFREAV DVEY+GYL
Sbjct: 314 TEEQMAIRK-DKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYL 373

Query: 367 IPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGNELAKLE 426
           IPKGWKV+PLFRNIHHS + F +P  FDPSRFEVAPKP TFMPFG+G H+CPGNELAKLE
Sbjct: 374 IPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLE 433

Query: 427 ILIMIHHLVTKFR 440
           + IMIHHL TK+R
Sbjct: 434 MSIMIHHLTTKYR 433

BLAST of MELO3C016752.jh1 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 514.6 bits (1324), Expect = 9.3e-146
Identity = 249/432 (57.64%), Postives = 327/432 (75.69%), Query Frame = 0

Query: 7   SILIFFLSLLWYISLKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQKR 66
           S+ ++FL  L  IS +R   + +     LPPG++GWPY+GET QLYSQ P+ FF +KQKR
Sbjct: 14  SLFLYFLRCL--ISQRRFGSSKL----PLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKR 73

Query: 67  YGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDYHC 126
           YG +FKTH+LGCPCVM++SPEAA+FVLVT++HLFKPT+P SKE ++G  A+FFHQGDYH 
Sbjct: 74  YGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHA 133

Query: 127 RLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIFGH 186
           +LRKLV  +   +SIR +VPDI+S+A  +L SW    +INT+ EMK  +F V +L+IFG 
Sbjct: 134 KLRKLVLRAFMPESIRNMVPDIESIAQDSLRSW-EGTMINTYQEMKTYTFNVALLSIFGK 193

Query: 187 LEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRLRE 246
            E  Y++DLK+ Y IL+ GYNS P N+PGT + K++ ARK LS+IL  I+ ER +     
Sbjct: 194 DEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSH 253

Query: 247 KDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQSL 306
            DLLGS +   ++     ++D QIADNIIGV+FAA+DTTASVM+WI+KYL + P +L+++
Sbjct: 254 NDLLGSFMGDKEE-----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAV 313

Query: 307 KAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKGYL 366
             EQ  I K + E  + L W  T+ MP+T +++ E+LR+ASI+S+TFREAV DVEY+GYL
Sbjct: 314 TEEQMAIRK-DKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYL 373

Query: 367 IPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGNELAKLE 426
           IPKGWKV+PLFRNIHHS + F +P  FDPSRFEVAPKP TFMPFG+G H+CPGNELAKLE
Sbjct: 374 IPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLE 432

Query: 427 ILIMIHHLVTKF 439
           + IMIHHL TK+
Sbjct: 434 MSIMIHHLTTKY 432

BLAST of MELO3C016752.jh1 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 514.6 bits (1324), Expect = 9.3e-146
Identity = 247/435 (56.78%), Postives = 331/435 (76.09%), Query Frame = 0

Query: 6   LSILIFFLSLLWYIS-LKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQ 65
           LS    FL LL +I+ ++R     +     LPPG++G+PY+GET QLYSQ P+ FFAAKQ
Sbjct: 10  LSAAALFLCLLRFIAGVRRSSSTKL----PLPPGTMGYPYVGETFQLYSQDPNVFFAAKQ 69

Query: 66  KRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDY 125
           +RYG +FKTH+LGCPCVM++SPEAA+FVLVT++HLFKPT+P SKE ++G  A+FFHQGDY
Sbjct: 70  RRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 129

Query: 126 HCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIF 185
           H +LRKLV  +   D+IR +VP I+S+A  +L+SW  ++ +NT+ EMK  +F V +++I 
Sbjct: 130 HSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSWDGTQ-LNTYQEMKTYTFNVALISIL 189

Query: 186 GHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRL 245
           G  E  Y++DLK+ Y IL+ GYNS P N+PGT + KA+ ARK L++IL +I+ +R +   
Sbjct: 190 GKDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPS 249

Query: 246 REKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQ 305
              DLLGS +     E +  ++D QIADNIIGV+FAA+DTTASV+TWI+KYL D P +L+
Sbjct: 250 SHTDLLGSFM-----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLE 309

Query: 306 SLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKG 365
           ++  EQ  I K + +  + L W  T+ MP+TY+++ E+LR A+I+S+TFREAV DVEY+G
Sbjct: 310 AVTEEQMAIRK-DKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEG 369

Query: 366 YLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGNELAK 425
           YLIPKGWKV+PLFRNIHH+ + F DP  FDPSRFEVAPKP TFMPFGSG+H+CPGNELAK
Sbjct: 370 YLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAK 429

Query: 426 LEILIMIHHLVTKFR 440
           LEI ++IHHL TK+R
Sbjct: 430 LEISVLIHHLTTKYR 433

BLAST of MELO3C016752.jh1 vs. TAIR 10
Match: AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 514.6 bits (1324), Expect = 9.3e-146
Identity = 247/435 (56.78%), Postives = 331/435 (76.09%), Query Frame = 0

Query: 6   LSILIFFLSLLWYIS-LKRDKKAAIRDGTKLPPGSLGWPYIGETLQLYSQHPDFFFAAKQ 65
           LS    FL LL +I+ ++R     +     LPPG++G+PY+GET QLYSQ P+ FFAAKQ
Sbjct: 10  LSAAALFLCLLRFIAGVRRSSSTKL----PLPPGTMGYPYVGETFQLYSQDPNVFFAAKQ 69

Query: 66  KRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPKSKELLIGPSALFFHQGDY 125
           +RYG +FKTH+LGCPCVM++SPEAA+FVLVT++HLFKPT+P SKE ++G  A+FFHQGDY
Sbjct: 70  RRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDY 129

Query: 126 HCRLRKLVQNSLSLDSIRTLVPDIDSVAASALDSWATSEVINTFHEMKKISFEVGILTIF 185
           H +LRKLV  +   D+IR +VP I+S+A  +L+SW  ++ +NT+ EMK  +F V +++I 
Sbjct: 130 HSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSWDGTQ-LNTYQEMKTYTFNVALISIL 189

Query: 186 GHLEAAYKDDLKKNYSILDAGYNSFPTNIPGTPYKKALSARKRLSKILGDIIHERGERRL 245
           G  E  Y++DLK+ Y IL+ GYNS P N+PGT + KA+ ARK L++IL +I+ +R +   
Sbjct: 190 GKDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPS 249

Query: 246 REKDLLGSLLNSIDDECEVKISDSQIADNIIGVLFAAQDTTASVMTWIVKYLHDQPKLLQ 305
              DLLGS +     E +  ++D QIADNIIGV+FAA+DTTASV+TWI+KYL D P +L+
Sbjct: 250 SHTDLLGSFM-----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLE 309

Query: 306 SLKAEQEEILKLNSENNQGLNWVQTRNMPITYKMVLESLRMASIISYTFREAVADVEYKG 365
           ++  EQ  I K + +  + L W  T+ MP+TY+++ E+LR A+I+S+TFREAV DVEY+G
Sbjct: 310 AVTEEQMAIRK-DKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEG 369

Query: 366 YLIPKGWKVMPLFRNIHHSPEYFPDPHNFDPSRFEVAPKPYTFMPFGSGVHACPGNELAK 425
           YLIPKGWKV+PLFRNIHH+ + F DP  FDPSRFEVAPKP TFMPFGSG+H+CPGNELAK
Sbjct: 370 YLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAK 429

Query: 426 LEILIMIHHLVTKFR 440
           LEI ++IHHL TK+R
Sbjct: 430 LEISVLIHHLTTKYR 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008452547.10.099.77PREDICTED: abscisic acid 8'-hydroxylase 4-like [Cucumis melo][more]
XP_004141255.11.31e-31497.04abscisic acid 8'-hydroxylase CYP707A1 [Cucumis sativus] >KGN55192.1 hypothetical... [more]
KAA0064383.15.34e-31399.76abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa][more]
TYK20204.17.62e-30197.18abscisic acid 8'-hydroxylase 4-like [Cucumis melo var. makuwa][more]
XP_038897319.16.02e-29992.71abscisic acid 8'-hydroxylase CYP707A1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A9QNE72.5e-17569.18Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A... [more]
Q9LJK27.7e-16967.13Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 ... [more]
Q0J1858.8e-16565.23Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70... [more]
A2Z2125.7e-16465.00Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
Q09J786.9e-16266.10Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A... [more]
Match NameE-valueIdentityDescription
A0A1S3BTH60.099.77abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo OX=3656 GN=LOC103493539 PE=3... [more]
A0A0A0L3K16.33e-31597.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639800 PE=3 SV=1[more]
A0A5A7V8C12.59e-31399.76Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A5D3D9K53.69e-30197.18Abscisic acid 8'-hydroxylase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A6J1IYR91.37e-27485.88abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111481123 ... [more]
Match NameE-valueIdentityDescription
AT3G19270.15.4e-17067.13cytochrome P450, family 707, subfamily A, polypeptide 4 [more]
AT4G19230.22.5e-14657.74cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT4G19230.19.3e-14657.64cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT5G45340.19.3e-14656.78cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT5G45340.29.3e-14656.78cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Harukei-3) v1.41
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 280..297
score: 30.07
coord: 337..348
score: 29.33
coord: 408..417
score: 47.45
coord: 417..428
score: 39.37
IPR001128Cytochrome P450PFAMPF00067p450coord: 36..440
e-value: 2.1E-66
score: 224.6
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 289..315
score: 17.62
coord: 376..400
score: 28.46
coord: 269..286
score: 21.19
coord: 417..440
score: 27.75
coord: 64..83
score: 29.92
coord: 407..417
score: 47.41
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 25..445
e-value: 5.1E-114
score: 383.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 33..445
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 8..438
e-value: 8.3E-26
score: 88.7
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 8..440
NoneNo IPR availablePANTHERPTHR24286:SF103CYTOCHROME P450 FAMILY ABA 8'-HYDROXYLASEcoord: 8..440
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 410..419

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C016752.jh1.t1MELO3C016752.jh1.t1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen