MELO3C014683.jh1 (gene) Melon (Harukei-3) v1.41

BLAST of MELO3C014683.jh1 vs. NCBI nr
Match: XP_004142170.3 (probable acyl-activating enzyme 18, peroxisomal [Cucumis sativus])

HSP 1 Score: 1399 bits (3621), Expect = 0.0
Identity = 688/725 (94.90%), Postives = 709/725 (97.79%), Query Frame = 0

Query: 1   MGKSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60
           MGKS+WEL +EDLVKTGLN+HEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP
Sbjct: 1   MGKSVWELDIEDLVKTGLNLHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60

Query: 61  FELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           +ELH LLYYSVYANWNISSNGPPPYWFPSI+QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSNVRWLPDSILNIAECCLLP 180
           FSLFQKLSYQSPEIYWSIVL ELSVTFQK PRCILDRT KSNVRWLPDSILNIAECCLLP
Sbjct: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180

Query: 181 SSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSRPLKDDNS AIVWR+EGCDNSNVN MTLKQLREKVTLVANALDATFSKGDAIAIDMPL
Sbjct: 181 SSRPLKDDNSSAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 IIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVTNIL 360
           +I+GGSCKAIVIPATGNRLDVDLR+QDLSW+EFLSTAKEFPRSNRYSPVYHS DGVTNIL
Sbjct: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSLLAG 420
           FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQ GDV CWPTNLGWVMGP+SVYSSLLAG
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480

Query: 481 SNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDEH 540
           SN+DDDLWLSS+SYYKPIFEFCGGTEL SSYI GTP+QPQAFGAF+TASMTTEFVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIPYPEDQPCIGEIGLFP+YLGASNELLNADHD+VYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600

Query: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVILVVL 660
           GY +VQGRADDTMNLGGIKTSSVEIERICDH DESILETAAVSLSPIEGGPEQLVILVVL
Sbjct: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660

Query: 661 KKQYVKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           +K+Y +SPQELK KFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 725
           LRSQL
Sbjct: 721 LRSQL 725

BLAST of MELO3C014683.jh1 vs. NCBI nr
Match: KAE8649475.1 (hypothetical protein Csa_018177 [Cucumis sativus])

HSP 1 Score: 1375 bits (3559), Expect = 0.0
Identity = 675/725 (93.10%), Postives = 705/725 (97.24%), Query Frame = 0

Query: 1   MGKSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60
           MGKS+WEL +EDLVKTGLN+HEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP
Sbjct: 1   MGKSVWELDIEDLVKTGLNLHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60

Query: 61  FELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           +ELH LLYYSVYANWNISSNGPPPYWFPSI+QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSNVRWLPDSILNIAECCLLP 180
           FSLFQKLSYQSPEIYWSIVL ELSVTFQK PRCILDRT KSNVRWLPDSILNIAECCLLP
Sbjct: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180

Query: 181 SSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSRPLKDDNS AIVWR+EGCDNSNVN MTLKQLREKVTLVANALDATFSKGDAIAIDMPL
Sbjct: 181 SSRPLKDDNSSAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 IIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVTNIL 360
           +I+GGSCKAIVIPATGNRLDVDLR+QDLSW+EFLSTAKEFPRSNRYSPVYHS DGVTNIL
Sbjct: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSLLAG 420
           FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQ GDV CWPTNLGWVMGP+SVYSSLLAG
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVT+LGTVPSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTMLGTVPSLVKTWKDTRCMEGLNWTKIRYFASTGET 480

Query: 481 SNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDEH 540
           SN+DDDLWLSS+SYYKP+FE CGGTELGSS+I G+PVQPQAFGAFSTASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPVQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP P+DQPCIGEIGLFP YLGAS+ELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 541 GIPSPDDQPCIGEIGLFPTYLGASSELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600

Query: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVILVVL 660
           GYL+VQGRADDTMNLGGIKTSSVE+ERICDH DES+LETAAV++SPI+GGPEQLVILVVL
Sbjct: 601 GYLVVQGRADDTMNLGGIKTSSVELERICDHVDESLLETAAVAVSPIQGGPEQLVILVVL 660

Query: 661 KKQYVKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           ++ Y KSPQELK KFSKAIQ NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDE+A
Sbjct: 661 REGYKKSPQELKIKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDEIA 720

Query: 721 LRSQL 725
           L+SQL
Sbjct: 721 LQSQL 725

BLAST of MELO3C014683.jh1 vs. NCBI nr
Match: KAA0040068.1 (putative acyl-activating enzyme 18, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 1364 bits (3531), Expect = 0.0
Identity = 671/725 (92.55%), Postives = 701/725 (96.69%), Query Frame = 0

Query: 1   MGKSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60
           MGKSIWELG+EDLV+TGLN+HEAVEFNRIL+NVLST QRSKPTEVWRELMARRALKPTHP
Sbjct: 1   MGKSIWELGIEDLVRTGLNLHEAVEFNRILKNVLSTTQRSKPTEVWRELMARRALKPTHP 60

Query: 61  FELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           FELHRLLYYSVYANWNIS++GPPPYWFPS+DQSKLTNLGKIMEL GPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTSGPPPYWFPSLDQSKLTNLGKIMELQGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSNVRWLPDSILNIAECCLLP 180
           FSLFQK S Q+PEIYWSIVLQELSVTFQKAPRCILDRT K N RWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDNSLAIVWRDEGCDNSNVN MTLKQLREKVTLVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 IIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVTNIL 360
           +IEGGSCKAIVIP TGNRL+VDLR+QDLSW+EFLSTAKEFPRSN YSP+YHS D VTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSLLAG 420
           FSSGTTG+PKAIPWTQLSPIRCAADSWAHMDVQAGDV CWPTNLGW MGP+SVYSSLL G
Sbjct: 361 FSSGTTGKPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVT+LGT+PSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTILGTIPSLVKTWKDTRCMEGLNWTKIRYFASTGET 480

Query: 481 SNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDEH 540
           SN+DDDLWLSS+SYYKP+FE CGGTELGSS+I G+PVQPQAFGAFSTASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPVQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP+PEDQPCIGEIGLFP+YLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDII+RTVG
Sbjct: 541 GIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIRRTVG 600

Query: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVILVVL 660
           GYLIVQGRADDTMNLGGIKTSSVEIERICDH DESILETAAVSLSPIEGGPEQLVILVVL
Sbjct: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660

Query: 661 KKQYVKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           KKQYVKS QELKDKFSKAIQ+NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 KKQYVKSSQELKDKFSKAIQRNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 725
           LRSQL
Sbjct: 721 LRSQL 725

BLAST of MELO3C014683.jh1 vs. NCBI nr
Match: XP_008449821.2 (PREDICTED: probable acyl-activating enzyme 18, peroxisomal [Cucumis melo] >KAA0040067.1 putative acyl-activating enzyme 18, peroxisomal [Cucumis melo var. makuwa] >TYK21692.1 putative acyl-activating enzyme 18, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 1345 bits (3482), Expect = 0.0
Identity = 661/725 (91.17%), Postives = 696/725 (96.00%), Query Frame = 0

Query: 1   MGKSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60
           MGKSIWELG+EDLVKTGLN+HEAVEFNRIL+NVLSTAQRSKP EVWRELMA+RALKPTHP
Sbjct: 1   MGKSIWELGIEDLVKTGLNLHEAVEFNRILKNVLSTAQRSKPAEVWRELMAKRALKPTHP 60

Query: 61  FELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           FELHRLLYYSVYANWNIS+NGPPPYWFPS++QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTNGPPPYWFPSLNQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSNVRWLPDSILNIAECCLLP 180
           FSLFQK S Q+PEIYWSIVLQELSVTFQKAPRCILDRT K N RWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDNSLAIVWRDEGCDNSNVN MTLKQLR+KVTLVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLRDKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 IIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVTNIL 360
           +IEGGSCKAIVIP TGNRL+VDLR+QDLSW+EFLSTAKEFPRSN YSP+YHS D VTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSLLAG 420
           FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDV CWPTNLGW MGP+SVYSSLL G
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVT+LGTVPSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTILGTVPSLVKTWKDTRCMEGLNWTKIRYFASTGET 480

Query: 481 SNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDEH 540
           SN+DDDLWLSS+SYYKP+FE CGGTELGSS+I G+P+QPQAFGAFSTASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPLQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP+PEDQPCIGEIGLFP+YLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 541 GIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600

Query: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVILVVL 660
           GYL+VQGRADDTMNLGGIKTSSVE+ERICD  DESILETAAV++SPI+GGPEQLVILVVL
Sbjct: 601 GYLVVQGRADDTMNLGGIKTSSVELERICDLVDESILETAAVAVSPIQGGPEQLVILVVL 660

Query: 661 KKQYVKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
            + Y KSPQELK KFSKAIQ NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 SEGYRKSPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 725
           L+S L
Sbjct: 721 LQSHL 725

BLAST of MELO3C014683.jh1 vs. NCBI nr
Match: XP_031740080.1 (probable acyl-activating enzyme 18, peroxisomal [Cucumis sativus])

HSP 1 Score: 1339 bits (3466), Expect = 0.0
Identity = 656/725 (90.48%), Postives = 694/725 (95.72%), Query Frame = 0

Query: 1   MGKSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60
           MGKSIWELG+EDLVKTGL +HEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP
Sbjct: 24  MGKSIWELGIEDLVKTGLKLHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 83

Query: 61  FELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           +ELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 84  YELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 143

Query: 121 FSLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSNVRWLPDSILNIAECCLLP 180
           FSLFQK S Q+PEIYWSIVLQELS+TFQKAPRCILDRT K N RWLPDS+LNIAECCL  
Sbjct: 144 FSLFQKFSIQNPEIYWSIVLQELSITFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 203

Query: 181 SSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDNSLAIVWRDEGCDNSNVN MTLKQLREKVTLVANALDATFSKGD +AIDMPL
Sbjct: 204 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDVVAIDMPL 263

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 264 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 323

Query: 301 IIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVTNIL 360
           +IEGGSCKAIVIPA+GNRL+VDLR+QDLSW+EF+STAKEFPRSN YSP+YHS D +TNIL
Sbjct: 324 VIEGGSCKAIVIPASGNRLEVDLREQDLSWEEFISTAKEFPRSNCYSPMYHSIDAMTNIL 383

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSLLAG 420
           FSSGTTGEPKAIPWTQ SPIRCAADSWAHMDVQ GDV CWPTNLGW MGP+SVYS+ L G
Sbjct: 384 FSSGTTGEPKAIPWTQRSPIRCAADSWAHMDVQPGDVFCWPTNLGWAMGPVSVYSAFLTG 443

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLG+GFGKFVQDAGVT+LGTVPSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 444 ATLALYHGSPLGYGFGKFVQDAGVTMLGTVPSLVKTWKDTRCMEGLNWTKIRYFASTGET 503

Query: 481 SNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDEH 540
           SN+DDDLWLSS+SYYKP+FE CGGTELGSS+I G+PVQPQAFGAFSTASMTT FVILDEH
Sbjct: 504 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPVQPQAFGAFSTASMTTGFVILDEH 563

Query: 541 GIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP P+DQPCIGEIGLFP YLGAS+ELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 564 GIPSPDDQPCIGEIGLFPTYLGASSELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 623

Query: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVILVVL 660
           GYL+VQGRADDTMNLGGIKTSSVE+ERICDH DES+LETAAV++SPI+GGPEQLVILVVL
Sbjct: 624 GYLVVQGRADDTMNLGGIKTSSVELERICDHVDESLLETAAVAVSPIQGGPEQLVILVVL 683

Query: 661 KKQYVKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           ++ Y KSPQELK KFSKAIQ NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDE+A
Sbjct: 684 REGYKKSPQELKIKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDEIA 743

Query: 721 LRSQL 725
           L+SQL
Sbjct: 744 LQSQL 748

BLAST of MELO3C014683.jh1 vs. ExPASy Swiss-Prot
Match: M4IRL9 (Probable CoA ligase CCL12 OS=Humulus lupulus OX=3486 GN=CCL12 PE=2 SV=1)

HSP 1 Score: 1054.3 bits (2725), Expect = 6.3e-307
Identity = 504/721 (69.90%), Postives = 608/721 (84.33%), Query Frame = 0

Query: 7   ELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHPFELHRL 66
           E+GVEDLVK GL+  EA  F R+L+++++ A+   P+EVWRE++ARR LKP+HP ELH+L
Sbjct: 3   EVGVEDLVKAGLSTEEAKYFQRVLEDIVNGAKGLDPSEVWREVVARRLLKPSHPHELHQL 62

Query: 67  LYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISSFSLFQK 126
           LY+SVYANW++S+ GPP YWFPS+ QSK TNLG++ME +G ++LG  YKDPI+SFSLF+K
Sbjct: 63  LYHSVYANWDVSTRGPPLYWFPSLHQSKKTNLGRLMEKYGSEVLGPLYKDPITSFSLFRK 122

Query: 127 LSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKS--NVRWLPDSILNIAECCLLPSSRP 186
            S Q PE YWSIVL+ELSV+FQ+AP+CILD   KS  +   LP S+LNIAECCLLP+S P
Sbjct: 123 FSVQQPEAYWSIVLKELSVSFQEAPKCILDSADKSKRSGSLLPGSVLNIAECCLLPTSYP 182

Query: 187 LKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDATFSKGDAIAIDMPLTVDA 246
           +K D+S AIVWRDEG D+S VN +TLK+LREKV LVANALDA FSKGDAIAIDMP+TV+A
Sbjct: 183 MKRDDSPAIVWRDEGYDDSEVNVLTLKELREKVMLVANALDAMFSKGDAIAIDMPMTVNA 242

Query: 247 VVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSRIIEG 306
           VVIYLAI+L+G VVVSIADSFAA E+A RLR+S AK IFTQDFILRGG+K+ LYSR+++ 
Sbjct: 243 VVIYLAIILSGFVVVSIADSFAAKEIATRLRVSNAKAIFTQDFILRGGRKFSLYSRVVDA 302

Query: 307 GSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVTNILFSSG 366
              KAIV+P  G+ L + LR+QD+SW++F+S      RSN Y+P Y   D + NILFSSG
Sbjct: 303 APHKAIVLPVNGSNLGLQLREQDISWEKFISNVSNQARSNHYTPFYQPVDSLINILFSSG 362

Query: 367 TTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSLLAGATLA 426
           TTGEPKAIPWTQLSP+RCAAD+WAH+D Q GDV CWPTNLGWVMGP+ ++SS L+GATLA
Sbjct: 363 TTGEPKAIPWTQLSPLRCAADTWAHIDAQVGDVFCWPTNLGWVMGPVLLFSSFLSGATLA 422

Query: 427 LYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGETSNID 486
           LYHGSPLGHGFGKFVQD+GVT+LGTVPSLVK WK+T+CM+GL+WTKI+ FASTGE SN+D
Sbjct: 423 LYHGSPLGHGFGKFVQDSGVTILGTVPSLVKAWKNTQCMKGLDWTKIKVFASTGEASNVD 482

Query: 487 DDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDEHGIPY 546
           DDLWLSS++YY+PI E CGGTEL SSYI G+ VQPQAFGAFSTA+MT   VILDE+GIPY
Sbjct: 483 DDLWLSSQAYYQPIIECCGGTELASSYIQGSLVQPQAFGAFSTAAMTVGLVILDENGIPY 542

Query: 547 PEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVGGYLI 606
           P+ Q C GE+GLFPLYLGA++ LLNAD+++VYFKGMP+YNGM LRRHGDI+KRT GG+ I
Sbjct: 543 PDKQECTGEVGLFPLYLGATDRLLNADNEKVYFKGMPLYNGMSLRRHGDILKRTAGGFFI 602

Query: 607 VQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVILVVLKKQY 666
           V GRADDTMNLGGIKTSS+EIER+C+  DES++ET AVS++P+ GGPEQLV+ VVLK  +
Sbjct: 603 VHGRADDTMNLGGIKTSSIEIERVCNRADESVMETTAVSVAPVSGGPEQLVMFVVLKSGH 662

Query: 667 VKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELALRSQ 726
               + LK KFSKAIQ NLNPLFKV FVKIV  FPRTASNKLLRRVLR QMK EL +RS+
Sbjct: 663 NNEAERLKKKFSKAIQSNLNPLFKVSFVKIVEEFPRTASNKLLRRVLRDQMKHELLVRSK 722

BLAST of MELO3C014683.jh1 vs. ExPASy Swiss-Prot
Match: Q84P17 (Probable acyl-activating enzyme 18, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE18 PE=2 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 1.2e-292
Identity = 485/728 (66.62%), Postives = 588/728 (80.77%), Query Frame = 0

Query: 1   MGKSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60
           M KSI EL  +D VK GL + +A EF++++ +V++ A  + P + W+ L+    LKP HP
Sbjct: 1   MWKSIGELSCDDYVKAGLTLEDAKEFDKLVSDVITKAIETDPRDQWKALVDESVLKPWHP 60

Query: 61  FELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
             LH+LLYYSVY+NW+ S +GPP YWFPS+ QSK TNLGK+ME HGP+LLG SYK+P+ S
Sbjct: 61  HPLHQLLYYSVYSNWDSSVHGPPLYWFPSLSQSKSTNLGKLMEYHGPRLLGPSYKNPLES 120

Query: 121 FSLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSNVRWLPDSILNIAECCLLP 180
           F LF++ S + PE+YWS V+ ELS+ F   PRCIL++ +K    WLPD++LNIAECCL+P
Sbjct: 121 FELFRRFSVEHPEVYWSFVIDELSLVFHTPPRCILNK-SKPEGTWLPDAVLNIAECCLMP 180

Query: 181 SSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SS P K+D+S+A+VWR+EG D+S VN MT+K+LRE+V LVANA+  +F KGD IAIDMP+
Sbjct: 181 SSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQVMLVANAISGSFEKGDTIAIDMPM 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAV+IYLAI+LAG +VVSIADSFAA E+A RL+ISKAKGIFTQD+ILRGG+++PLYSR
Sbjct: 241 TVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISKAKGIFTQDYILRGGRRFPLYSR 300

Query: 301 IIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPR-SNRYSPVYHSRDGVTNI 360
           ++E    K IV+PA+G  L V LR+QD+SW +FLS AK      N Y P+Y   + V NI
Sbjct: 301 VVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAKPHSSGENYYRPIYLPVESVINI 360

Query: 361 LFSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSLLA 420
           LFSSGTTGEPKAIPWTQLSPIR A D WAH+DVQ G   CWPTNLGWVMGP  ++S  L 
Sbjct: 361 LFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHTYCWPTNLGWVMGPTLMFSCFLT 420

Query: 421 GATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGE 480
           GATLALY GSPLG GFGKFVQDAGVT+LGTVPSLVKTWK T CMEGLNWTKI+ FA+TGE
Sbjct: 421 GATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKTWKRTNCMEGLNWTKIKFFATTGE 480

Query: 481 TSNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDE 540
            SN+DD LWLSSK+ YKP+ E CGGTEL SSYI G+P+QPQAFGAFST SMTT  +I DE
Sbjct: 481 ASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPLQPQAFGAFSTPSMTTRIIIFDE 540

Query: 541 HGIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTV 600
           +G+PYP+DQPC GE+GLFP +LGA++ LLNA+HDEVYFKGMP+Y   +LRRHGDI+KRTV
Sbjct: 541 NGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVYFKGMPMYKETRLRRHGDIVKRTV 600

Query: 601 GGYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVILVV 660
           GGY  VQGRADDTMNLGGIKTSS+EIER+CD  DE I ETAAV+L+P  GGPE LVI  V
Sbjct: 601 GGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECISETAAVTLTPPNGGPELLVIFAV 660

Query: 661 LKKQY-VKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDE 720
           LK+ +  +S +ELK KFS+ IQK+LNPLFKV FVKIVP FPRTAS+KLLRRVLR Q+K E
Sbjct: 661 LKEGFKQQSGEELKMKFSRTIQKDLNPLFKVSFVKIVPEFPRTASSKLLRRVLRDQIKQE 720

Query: 721 -LALRSQL 726
            L+LRS++
Sbjct: 721 LLSLRSRI 727

BLAST of MELO3C014683.jh1 vs. ExPASy Swiss-Prot
Match: F4KBF3 (Probable acyl-activating enzyme 17, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE17 PE=2 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 7.2e-202
Identity = 363/730 (49.73%), Postives = 492/730 (67.40%), Query Frame = 0

Query: 3   KSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHPFE 62
           KS+  +   D+   G++   + +  R L++++  +  S P E W ++ +RR L P  PF 
Sbjct: 4   KSLNSITKSDIEALGISGDVSEKLLRDLEDIIHGS--STPPETWIQI-SRRILHPNLPFS 63

Query: 63  LHRLLYYSVYANWNISSNGPP-PYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISSF 122
            H+++YY  Y ++     GP  P W P    + LTN+GK++E  G + LG +YK+P+SSF
Sbjct: 64  FHQMMYYGCYKDF-----GPDLPAWIPDPKVASLTNVGKLLEKRGKEFLGGNYKNPVSSF 123

Query: 123 SLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSN---VRWLPDSILNIAECCL 182
           S FQ+ S  +PE+YW  VL EL++ F   P+CIL++    +    +WLP + LN A  CL
Sbjct: 124 SSFQEFSVSNPEVYWKTVLDELNILFSVPPKCILEKDTSGDNPGGKWLPGAYLNPARNCL 183

Query: 183 LPSSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDAT-FSKGDAIAID 242
               +   DD  + I WRDEG D+  VN+MTL +LR +V L A+AL A    +  AIA+D
Sbjct: 184 TNGFKRRLDD--IVIRWRDEGSDDLPVNTMTLLELRSQVWLAAHALSALGLEEESAIAVD 243

Query: 243 MPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPL 302
           MP+ V++V+IYLAIVLAG VVVSIADSF+  E++ RL+ISKAK IFTQD I+RG K  PL
Sbjct: 244 MPMNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKISKAKAIFTQDVIIRGDKSIPL 303

Query: 303 YSRIIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVT 362
           Y R+++  +  AIV+PA G+   + LR++DLSW  FL  A+   R   Y          T
Sbjct: 304 YRRVVDAEAPLAIVVPARGSSCRMKLREKDLSWNNFLGNARNL-RGVEYVAAEKPAGAYT 363

Query: 363 NILFSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSL 422
           NILFSSGTTGEPKAIPWT +SP++ AAD+W H+DVQ GDV+ WPTNLGW+MGP  VY+SL
Sbjct: 364 NILFSSGTTGEPKAIPWTNISPLKSAADAWCHLDVQRGDVVAWPTNLGWMMGPWLVYASL 423

Query: 423 LAGATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFAST 482
           + GA + LY+GSPLG  F KFVQDA V++LG +PS+V+TW+++    G +W++IR F ST
Sbjct: 424 INGACMGLYNGSPLGPTFAKFVQDAEVSVLGVIPSIVRTWQNSNSTSGYDWSRIRCFGST 483

Query: 483 GETSNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVIL 542
           GE SNID+ LWL  +++YKP+ E+CGGTE+G S+I+G+ +QPQ+  AFSTA+M  +  IL
Sbjct: 484 GEASNIDEYLWLMGRAHYKPVIEYCGGTEIGGSFISGSLLQPQSLAAFSTAAMGCKLFIL 543

Query: 543 DEHGIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKR 602
           DE   P P     +GE+ L P   GAS+ LLN +H +VYF+GMP + G  LRRHGD+ +R
Sbjct: 544 DEDSNPIPPYAAGVGELALCPHMFGASSTLLNGNHFKVYFQGMPTFQGQILRRHGDLFER 603

Query: 603 TVGGYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVIL 662
           T  GY    GRADDTMNLGGIK  S+EIER+C+  D+S+LETAA+ + P  GGPEQLVI 
Sbjct: 604 TSKGYYRAHGRADDTMNLGGIKVGSIEIERVCNSVDDSVLETAAIGVPPPSGGPEQLVIA 663

Query: 663 VVLKKQYVKSP--QELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQM 722
           VV K     +P    LK  F+  IQK LNPLFKV  V  +PS PRTA+NK++RRVLR+Q+
Sbjct: 664 VVFKSPEFSNPDLNLLKKSFNSEIQKKLNPLFKVSSVVTLPSLPRTATNKVMRRVLRQQL 721

Query: 723 KDELALRSQL 726
             +  L S+L
Sbjct: 724 -TQTGLNSKL 721

BLAST of MELO3C014683.jh1 vs. ExPASy Swiss-Prot
Match: Q15YI8 (Acetyl-coenzyme A synthetase OS=Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087) OX=342610 GN=acsA PE=3 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 9.5e-37
Identity = 161/621 (25.93%), Postives = 267/621 (43.00%), Query Frame = 0

Query: 128 SYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKS---NVRWLPDSILNIAECCLLPSSRP 187
           S + PE++W+   Q  S+ + K P  I + +  +   +++W  D  LN+A  C+      
Sbjct: 30  SVEQPEVFWA--EQAKSLDWVKTPTKIKNTSFDAHHVSIKWFEDGELNVAYNCI--DRHL 89

Query: 188 LKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANAL-DATFSKGDAIAIDMPLTVD 247
            K  +  A +W  EG D  + + +T  +L ++V  +AN L      KGD +AI MP+   
Sbjct: 90  AKRGHVTAFIW--EGDDPHSHDIITYHRLHDEVAKIANGLRKLGVGKGDRVAIYMPMIPQ 149

Query: 248 AVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSRI-- 307
           AV   LA    G V   I   F+ N +A R+   +AK + T D  LR GK  PL   +  
Sbjct: 150 AVYAMLACARIGAVHTVIFGGFSPNAIADRVNNCQAKVLITADEGLRAGKHIPLKDNVDT 209

Query: 308 -IEGGSC----KAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGV 367
            +    C    + IV   TG+   V   K D+ W +          S    P   + +  
Sbjct: 210 ALAEHDCPSMEQVIVFKHTGSH--VSWGKDDVWWHDLTDGC-----STDCPPEVMNAEDP 269

Query: 368 TNILFSSGTTGEPKAIPWTQLSPIRCAADSWAHM-DVQAGDVLCWPTNLGWVMG-PISVY 427
             IL++SG+TG+PK +  T    +  A+ +  ++ D + GD+     ++GWV G    VY
Sbjct: 270 LFILYTSGSTGQPKGVVHTTGGYLLWASITHKYVFDYKPGDIYWCAADVGWVTGHSYIVY 329

Query: 428 SSLLAGATLALYHGSPL---GHGFGKFVQDAGVTLLGTVPSLVKTW--KDTRCMEGLNWT 487
             L  GAT  ++ G P        G+ V    V +L T P+ ++          EG++  
Sbjct: 330 GPLANGATSVMFEGVPTYPDVRRIGQIVDKHKVNILYTAPTAIRALMAHGDFPTEGISGE 389

Query: 488 KIRHFASTGETSNIDDDLWLSSKSYYK-------PIFEFCGGTELGSSYITGTP-VQPQA 547
            +R   S GE  N +   W     YY        PI +    TE G++ +T  P V    
Sbjct: 390 SLRLLGSVGEPINPEAWHW-----YYTTVGQSRCPIVDTWWQTETGAAMLTPLPSVTAMK 449

Query: 548 FGAFSTASMTTEFVILDEHGIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMP 607
            GA S      +  ++D  G          G + +   + G +  +   DH+        
Sbjct: 450 PGAASHPFFGVQPALVDGQGNEL--SGATEGNLIITDSWPGQARTVY-GDHERFVQTYFT 509

Query: 608 IYNGMQLRRHGDIIKRTVGGYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAA 667
            Y G      GD  +R    Y  + GR DD +N+ G +  + EIE        +++   A
Sbjct: 510 TYPGTYCT--GDGARRDEDDYFWITGRVDDVLNVSGHRLGTAEIE-------SALVSHNA 569

Query: 668 VSLSPIEGGPEQL----VILVVLKKQYVKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPS 719
           V+ + + G P  L    + + ++  + V+   EL  + S  ++K L+P+     ++    
Sbjct: 570 VAEAAVVGYPHDLKGQGIYVYLMPNEGVEITDELTKEVSNWVRKELSPIATPDLIQWSSG 620

BLAST of MELO3C014683.jh1 vs. ExPASy Swiss-Prot
Match: A1JIK3 (Acetyl-coenzyme A synthetase OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081) OX=393305 GN=acs PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 1.2e-36
Identity = 153/571 (26.80%), Postives = 246/571 (43.08%), Query Frame = 0

Query: 162 NVRWLPDSILNIAECCLLPSSRPLKD-DNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLV 221
           ++RW  D  LN+A  CL    R L +  +  AI+W  EG D +   ++T KQL   V   
Sbjct: 66  SIRWFEDGTLNLAANCL---DRHLAERGDQTAIIW--EGDDPNQSKTVTYKQLHHDVCQF 125

Query: 222 ANALDAT-FSKGDAIAIDMPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKA 281
           AN L      KGD +AI MP+  +A V  LA    G V   I   F+ + VA R+  S +
Sbjct: 126 ANVLKKLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPDAVAGRIIDSNS 185

Query: 282 KGIFTQDFILRGGKKYPLYSRIIEG------GSCKAIVIPATGNRLDVDLRKQDLSWKEF 341
           K + T D  +R G+  PL   + E        S K +V+             +D+ W + 
Sbjct: 186 KLVITADEGIRAGRAIPLKKNVDEALKNPAITSIKNVVVFQRTGNASYWKDGRDVWWHDL 245

Query: 342 LSTAKEFPRSNRYSPVYHSRDGVTNILFSSGTTGEPKAIPWTQLSPIRCAADSWAHM-DV 401
           +  A     S    P   + +    IL++SG+TG+PK +  T    +  AA ++ ++ D 
Sbjct: 246 IKDA-----SVDCPPEEMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAALTFKYVFDY 305

Query: 402 QAGDVLCWPTNLGWVMG-PISVYSSLLAGATLALYHGSPLGHG---FGKFVQDAGVTLLG 461
             GD+     ++GWV G    +Y  L  GA   ++ G P   G    G+ +    V +L 
Sbjct: 306 HPGDIYWCTADVGWVTGHSYLLYGPLACGAITLMFEGVPNYPGVNRLGQVIDKHQVNILY 365

Query: 462 TVPSLVKTW--KDTRCMEGLNWTKIRHFASTGETSNIDDDLWLSSK--SYYKPIFEFCGG 521
           T P+ ++    +  + +EG   T +R   S GE  N +   W  +K  +   PI +    
Sbjct: 366 TAPTAIRALMAEGDKAIEGTKRTSLRIMGSVGEPINPEAWEWYYNKIGNSKCPIVDTWWQ 425

Query: 522 TELGSSYITGTPVQPQ-AFGAFSTASMTTEFVILDEHGIPYPEDQPCIGEIGLFPLYLGA 581
           TE G   IT  P   +   G+ +      +  ++D  G   P++    G + +   + G 
Sbjct: 426 TETGGFMITPLPGATELKAGSATRPFFGVQPALVDNLG--NPQEGAAEGNLVITDSWPGQ 485

Query: 582 SNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVGGYLIVQGRADDTMNLGGIKTSSV 641
           +  L   DH+         + GM     GD  +R   GY  + GR DD +N+ G +  + 
Sbjct: 486 ARTLF-GDHERFEQTYFSTFKGMYF--SGDGARRDEDGYYWITGRVDDVLNVSGHRLGTA 545

Query: 642 EIE-RICDHFDESILETAAVSLSPIEGGPEQLVILVVLKKQYVKSPQELKDKFSKAIQKN 701
           EIE  +  H    I E A V +     G  Q +   +      +   EL  +    ++K 
Sbjct: 546 EIESALVSH--PKIAEAAVVGVPHNIKG--QAIYAYITLNHGEEPTPELYTEVRNWVRKE 605

Query: 702 LNPLFKVGFVKIVPSFPRTASNKLLRRVLRK 714
           + P+     +    S P+T S K++RR+LRK
Sbjct: 606 IGPIATPDILHWTDSLPKTRSGKIMRRILRK 617

BLAST of MELO3C014683.jh1 vs. ExPASy TrEMBL
Match: A0A0A0L1Q9 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280430 PE=4 SV=1)

HSP 1 Score: 1394 bits (3609), Expect = 0.0
Identity = 686/725 (94.62%), Postives = 708/725 (97.66%), Query Frame = 0

Query: 1   MGKSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60
           MGKS+WEL +EDLVKTGLN+HEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP
Sbjct: 1   MGKSVWELDIEDLVKTGLNLHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60

Query: 61  FELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           +ELH LLYYSVYANWNISSNGPPPYWFPSI+QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSNVRWLPDSILNIAECCLLP 180
           FSLFQKLSYQSPEIYWSIVL ELSVTFQK PRCILDRT KSNVRWLPDSILNIAECCLLP
Sbjct: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180

Query: 181 SSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSRPLKDDNS AIVWR+EGCDNSNVN MTLKQLREKVTLVANALDATFSKGDAIAIDMPL
Sbjct: 181 SSRPLKDDNSSAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 IIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVTNIL 360
           +I+GGSCKAIVIPATGNRLDVDLR+QDLSW+EFLSTAKEFPRSNRYSPVYHS DGVTNIL
Sbjct: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSLLAG 420
           FSSGTTGEPKAIPWTQ SPIRCAADSWAHMDVQ GDV CWPTNLGWVMGP+SVYSSLLAG
Sbjct: 361 FSSGTTGEPKAIPWTQRSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLG+GFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET
Sbjct: 421 ATLALYHGSPLGYGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480

Query: 481 SNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDEH 540
           SN+DDDLWLSS+SYYKPIFEFCGGTEL SSYI GTP+QPQAFGAF+TASMTTEFVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTTASMTTEFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIPYPEDQPCIGEIGLFP+YLGASNELLNADHD+VYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 541 GIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQLRRHGDIIKRTVG 600

Query: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVILVVL 660
           GY +VQGRADDTMNLGGIKTSSVEIERICDH DESILETAAVSLSPIEGGPEQLVILVVL
Sbjct: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660

Query: 661 KKQYVKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           +K+Y +SPQELK KFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 RKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 725
           LRSQL
Sbjct: 721 LRSQL 725

BLAST of MELO3C014683.jh1 vs. ExPASy TrEMBL
Match: A0A5A7TAS8 (Putative acyl-activating enzyme 18, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00370 PE=3 SV=1)

HSP 1 Score: 1364 bits (3531), Expect = 0.0
Identity = 671/725 (92.55%), Postives = 701/725 (96.69%), Query Frame = 0

Query: 1   MGKSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60
           MGKSIWELG+EDLV+TGLN+HEAVEFNRIL+NVLST QRSKPTEVWRELMARRALKPTHP
Sbjct: 1   MGKSIWELGIEDLVRTGLNLHEAVEFNRILKNVLSTTQRSKPTEVWRELMARRALKPTHP 60

Query: 61  FELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           FELHRLLYYSVYANWNIS++GPPPYWFPS+DQSKLTNLGKIMEL GPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTSGPPPYWFPSLDQSKLTNLGKIMELQGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSNVRWLPDSILNIAECCLLP 180
           FSLFQK S Q+PEIYWSIVLQELSVTFQKAPRCILDRT K N RWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDNSLAIVWRDEGCDNSNVN MTLKQLREKVTLVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 IIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVTNIL 360
           +IEGGSCKAIVIP TGNRL+VDLR+QDLSW+EFLSTAKEFPRSN YSP+YHS D VTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSLLAG 420
           FSSGTTG+PKAIPWTQLSPIRCAADSWAHMDVQAGDV CWPTNLGW MGP+SVYSSLL G
Sbjct: 361 FSSGTTGKPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVT+LGT+PSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTILGTIPSLVKTWKDTRCMEGLNWTKIRYFASTGET 480

Query: 481 SNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDEH 540
           SN+DDDLWLSS+SYYKP+FE CGGTELGSS+I G+PVQPQAFGAFSTASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPVQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP+PEDQPCIGEIGLFP+YLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDII+RTVG
Sbjct: 541 GIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIRRTVG 600

Query: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVILVVL 660
           GYLIVQGRADDTMNLGGIKTSSVEIERICDH DESILETAAVSLSPIEGGPEQLVILVVL
Sbjct: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPIEGGPEQLVILVVL 660

Query: 661 KKQYVKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           KKQYVKS QELKDKFSKAIQ+NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 KKQYVKSSQELKDKFSKAIQRNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 725
           LRSQL
Sbjct: 721 LRSQL 725

BLAST of MELO3C014683.jh1 vs. ExPASy TrEMBL
Match: A0A5D3DDN3 (Putative acyl-activating enzyme 18, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00570 PE=3 SV=1)

HSP 1 Score: 1345 bits (3482), Expect = 0.0
Identity = 661/725 (91.17%), Postives = 696/725 (96.00%), Query Frame = 0

Query: 1   MGKSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60
           MGKSIWELG+EDLVKTGLN+HEAVEFNRIL+NVLSTAQRSKP EVWRELMA+RALKPTHP
Sbjct: 1   MGKSIWELGIEDLVKTGLNLHEAVEFNRILKNVLSTAQRSKPAEVWRELMAKRALKPTHP 60

Query: 61  FELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           FELHRLLYYSVYANWNIS+NGPPPYWFPS++QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTNGPPPYWFPSLNQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSNVRWLPDSILNIAECCLLP 180
           FSLFQK S Q+PEIYWSIVLQELSVTFQKAPRCILDRT K N RWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDNSLAIVWRDEGCDNSNVN MTLKQLR+KVTLVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLRDKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 IIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVTNIL 360
           +IEGGSCKAIVIP TGNRL+VDLR+QDLSW+EFLSTAKEFPRSN YSP+YHS D VTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSLLAG 420
           FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDV CWPTNLGW MGP+SVYSSLL G
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVT+LGTVPSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTILGTVPSLVKTWKDTRCMEGLNWTKIRYFASTGET 480

Query: 481 SNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDEH 540
           SN+DDDLWLSS+SYYKP+FE CGGTELGSS+I G+P+QPQAFGAFSTASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPLQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP+PEDQPCIGEIGLFP+YLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 541 GIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600

Query: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVILVVL 660
           GYL+VQGRADDTMNLGGIKTSSVE+ERICD  DESILETAAV++SPI+GGPEQLVILVVL
Sbjct: 601 GYLVVQGRADDTMNLGGIKTSSVELERICDLVDESILETAAVAVSPIQGGPEQLVILVVL 660

Query: 661 KKQYVKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
            + Y KSPQELK KFSKAIQ NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 SEGYRKSPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 725
           L+S L
Sbjct: 721 LQSHL 725

BLAST of MELO3C014683.jh1 vs. ExPASy TrEMBL
Match: A0A1S3BNK5 (probable acyl-activating enzyme 18, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103491592 PE=3 SV=1)

HSP 1 Score: 1345 bits (3482), Expect = 0.0
Identity = 661/725 (91.17%), Postives = 696/725 (96.00%), Query Frame = 0

Query: 1   MGKSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60
           MGKSIWELG+EDLVKTGLN+HEAVEFNRIL+NVLSTAQRSKP EVWRELMA+RALKPTHP
Sbjct: 1   MGKSIWELGIEDLVKTGLNLHEAVEFNRILKNVLSTAQRSKPAEVWRELMAKRALKPTHP 60

Query: 61  FELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           FELHRLLYYSVYANWNIS+NGPPPYWFPS++QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTNGPPPYWFPSLNQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSNVRWLPDSILNIAECCLLP 180
           FSLFQK S Q+PEIYWSIVLQELSVTFQKAPRCILDRT K N RWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDNSLAIVWRDEGCDNSNVN MTLKQLR+KVTLVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLRDKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 IIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVTNIL 360
           +IEGGSCKAIVIP TGNRL+VDLR+QDLSW+EFLSTAKEFPRSN YSP+YHS D VTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSLLAG 420
           FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDV CWPTNLGW MGP+SVYSSLL G
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLGHGFGKFVQDAGVT+LGTVPSLVKTWKDTRCMEGLNWTKIR+FASTGET
Sbjct: 421 ATLALYHGSPLGHGFGKFVQDAGVTILGTVPSLVKTWKDTRCMEGLNWTKIRYFASTGET 480

Query: 481 SNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDEH 540
           SN+DDDLWLSS+SYYKP+FE CGGTELGSS+I G+P+QPQAFGAFSTASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPLQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           GIP+PEDQPCIGEIGLFP+YLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG
Sbjct: 541 GIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600

Query: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVILVVL 660
           GYL+VQGRADDTMNLGGIKTSSVE+ERICD  DESILETAAV++SPI+GGPEQLVILVVL
Sbjct: 601 GYLVVQGRADDTMNLGGIKTSSVELERICDLVDESILETAAVAVSPIQGGPEQLVILVVL 660

Query: 661 KKQYVKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
            + Y KSPQELK KFSKAIQ NLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 SEGYRKSPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 725
           L+S L
Sbjct: 721 LQSHL 725

BLAST of MELO3C014683.jh1 vs. ExPASy TrEMBL
Match: A0A6J1DJZ2 (probable acyl-activating enzyme 18, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111021232 PE=4 SV=1)

HSP 1 Score: 1265 bits (3274), Expect = 0.0
Identity = 620/725 (85.52%), Postives = 668/725 (92.14%), Query Frame = 0

Query: 1   MGKSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60
           MGK+IWELGVED VK GLN+ EAVEF+RILQNV+   +RS P EVWRELMARR LKPTHP
Sbjct: 1   MGKNIWELGVEDTVKAGLNLEEAVEFDRILQNVVLVPKRSDPREVWRELMARRVLKPTHP 60

Query: 61  FELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           + LH+L+YYSVYANW+ SSNGPPPYWFPS+DQSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  YGLHQLVYYSVYANWDSSSNGPPPYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSNVRWLPDSILNIAECCLLP 180
           +SLFQK S Q PEIYWSIVL+ELSVTF K PRCILD   KS  RWLPDSILNIAECCLLP
Sbjct: 121 YSLFQKFSSQHPEIYWSIVLEELSVTFHKEPRCILDMAEKSKGRWLPDSILNIAECCLLP 180

Query: 181 SSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SSRPL+DDNSLAIVWRDEG DNSNVN MTLK+LREKVTLVANALDA FSKGDAIAIDMP+
Sbjct: 181 SSRPLRDDNSLAIVWRDEGGDNSNVNRMTLKELREKVTLVANALDARFSKGDAIAIDMPM 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TV AV+IYLAIVLAG VVVSIADSF+ANEVAVRLRIS AKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVHAVIIYLAIVLAGFVVVSIADSFSANEVAVRLRISNAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 IIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVTNIL 360
           +IEGGSCKAIVIPATGN L+VDLR+QDLSW+EFLSTAKE PRSN YS VYHS D +TNIL
Sbjct: 301 VIEGGSCKAIVIPATGNTLEVDLREQDLSWEEFLSTAKELPRSNCYSAVYHSIDAMTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSLLAG 420
           FSSGTTG+PKAIPWTQLSPIR  AD+WAH+D+  GD+LCWPTNLGWV+GP S+YS LL G
Sbjct: 361 FSSGTTGDPKAIPWTQLSPIRAVADAWAHIDIHVGDILCWPTNLGWVVGPTSLYSCLLTG 420

Query: 421 ATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGET 480
           ATLALYHGSPLG GFGKFV+DAGVTLLGTVPSLVKTWKDT+CMEGL+WTKIR+F STGET
Sbjct: 421 ATLALYHGSPLGRGFGKFVEDAGVTLLGTVPSLVKTWKDTQCMEGLDWTKIRYFTSTGET 480

Query: 481 SNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDEH 540
           SN+DDDLWLSS+SYYKPI E CGGTEL SSYI G+P+QPQAFGAFSTASMTT FVILDEH
Sbjct: 481 SNVDDDLWLSSRSYYKPIIECCGGTELASSYIMGSPLQPQAFGAFSTASMTTGFVILDEH 540

Query: 541 GIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTVG 600
           G+PYPEDQPC+GE+GLFPLYLGAS+ LLNADHDEVYFKGMPIYN MQLRRHGDIIKRTVG
Sbjct: 541 GMPYPEDQPCVGELGLFPLYLGASDTLLNADHDEVYFKGMPIYNEMQLRRHGDIIKRTVG 600

Query: 601 GYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVILVVL 660
           GY +VQGRADDTMNLGGIKTSSVEIERICDH +ESILETAAV +SPIEGGPEQLVILVVL
Sbjct: 601 GYFVVQGRADDTMNLGGIKTSSVEIERICDHAEESILETAAVGVSPIEGGPEQLVILVVL 660

Query: 661 KKQYVKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDELA 720
           +K Y +SPQELK KFSKAIQ  LNPLFKVGFVKIVP+FPRTASNKLLRRVLRKQMKDELA
Sbjct: 661 RKGYRRSPQELKIKFSKAIQSKLNPLFKVGFVKIVPNFPRTASNKLLRRVLRKQMKDELA 720

Query: 721 LRSQL 725
           +RSQL
Sbjct: 721 VRSQL 725

BLAST of MELO3C014683.jh1 vs. TAIR 10
Match: AT1G55320.1 (acyl-activating enzyme 18 )

HSP 1 Score: 1006.9 bits (2602), Expect = 8.2e-294
Identity = 485/728 (66.62%), Postives = 588/728 (80.77%), Query Frame = 0

Query: 1   MGKSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60
           M KSI EL  +D VK GL + +A EF++++ +V++ A  + P + W+ L+    LKP HP
Sbjct: 1   MWKSIGELSCDDYVKAGLTLEDAKEFDKLVSDVITKAIETDPRDQWKALVDESVLKPWHP 60

Query: 61  FELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
             LH+LLYYSVY+NW+ S +GPP YWFPS+ QSK TNLGK+ME HGP+LLG SYK+P+ S
Sbjct: 61  HPLHQLLYYSVYSNWDSSVHGPPLYWFPSLSQSKSTNLGKLMEYHGPRLLGPSYKNPLES 120

Query: 121 FSLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSNVRWLPDSILNIAECCLLP 180
           F LF++ S + PE+YWS V+ ELS+ F   PRCIL++ +K    WLPD++LNIAECCL+P
Sbjct: 121 FELFRRFSVEHPEVYWSFVIDELSLVFHTPPRCILNK-SKPEGTWLPDAVLNIAECCLMP 180

Query: 181 SSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240
           SS P K+D+S+A+VWR+EG D+S VN MT+K+LRE+V LVANA+  +F KGD IAIDMP+
Sbjct: 181 SSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQVMLVANAISGSFEKGDTIAIDMPM 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAV+IYLAI+LAG +VVSIADSFAA E+A RL+ISKAKGIFTQD+ILRGG+++PLYSR
Sbjct: 241 TVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISKAKGIFTQDYILRGGRRFPLYSR 300

Query: 301 IIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPR-SNRYSPVYHSRDGVTNI 360
           ++E    K IV+PA+G  L V LR+QD+SW +FLS AK      N Y P+Y   + V NI
Sbjct: 301 VVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAKPHSSGENYYRPIYLPVESVINI 360

Query: 361 LFSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSLLA 420
           LFSSGTTGEPKAIPWTQLSPIR A D WAH+DVQ G   CWPTNLGWVMGP  ++S  L 
Sbjct: 361 LFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHTYCWPTNLGWVMGPTLMFSCFLT 420

Query: 421 GATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFASTGE 480
           GATLALY GSPLG GFGKFVQDAGVT+LGTVPSLVKTWK T CMEGLNWTKI+ FA+TGE
Sbjct: 421 GATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKTWKRTNCMEGLNWTKIKFFATTGE 480

Query: 481 TSNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDE 540
            SN+DD LWLSSK+ YKP+ E CGGTEL SSYI G+P+QPQAFGAFST SMTT  +I DE
Sbjct: 481 ASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPLQPQAFGAFSTPSMTTRIIIFDE 540

Query: 541 HGIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKRTV 600
           +G+PYP+DQPC GE+GLFP +LGA++ LLNA+HDEVYFKGMP+Y   +LRRHGDI+KRTV
Sbjct: 541 NGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVYFKGMPMYKETRLRRHGDIVKRTV 600

Query: 601 GGYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVILVV 660
           GGY  VQGRADDTMNLGGIKTSS+EIER+CD  DE I ETAAV+L+P  GGPE LVI  V
Sbjct: 601 GGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECISETAAVTLTPPNGGPELLVIFAV 660

Query: 661 LKKQY-VKSPQELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDE 720
           LK+ +  +S +ELK KFS+ IQK+LNPLFKV FVKIVP FPRTAS+KLLRRVLR Q+K E
Sbjct: 661 LKEGFKQQSGEELKMKFSRTIQKDLNPLFKVSFVKIVPEFPRTASSKLLRRVLRDQIKQE 720

Query: 721 -LALRSQL 726
            L+LRS++
Sbjct: 721 LLSLRSRI 727

BLAST of MELO3C014683.jh1 vs. TAIR 10
Match: AT1G55320.2 (acyl-activating enzyme 18 )

HSP 1 Score: 742.7 bits (1916), Expect = 2.9e-214
Identity = 364/510 (71.37%), Postives = 423/510 (82.94%), Query Frame = 0

Query: 219 LVANALDATFSKGDAIAIDMPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISK 278
           LVANA+  +F KGD IAIDMP+TVDAV+IYLAI+LAG +VVSIADSFAA E+A RL+ISK
Sbjct: 2   LVANAISGSFEKGDTIAIDMPMTVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISK 61

Query: 279 AKGIFTQDFILRGGKKYPLYSRIIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAK 338
           AKGIFTQD+ILRGG+++PLYSR++E    K IV+PA+G  L V LR+QD+SW +FLS AK
Sbjct: 62  AKGIFTQDYILRGGRRFPLYSRVVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAK 121

Query: 339 EFPR-SNRYSPVYHSRDGVTNILFSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDV 398
                 N Y P+Y   + V NILFSSGTTGEPKAIPWTQLSPIR A D WAH+DVQ G  
Sbjct: 122 PHSSGENYYRPIYLPVESVINILFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHT 181

Query: 399 LCWPTNLGWVMGPISVYSSLLAGATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTW 458
            CWPTNLGWVMGP  ++S  L GATLALY GSPLG GFGKFVQDAGVT+LGTVPSLVKTW
Sbjct: 182 YCWPTNLGWVMGPTLMFSCFLTGATLALYSGSPLGRGFGKFVQDAGVTVLGTVPSLVKTW 241

Query: 459 KDTRCMEGLNWTKIRHFASTGETSNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPV 518
           K T CMEGLNWTKI+ FA+TGE SN+DD LWLSSK+ YKP+ E CGGTEL SSYI G+P+
Sbjct: 242 KRTNCMEGLNWTKIKFFATTGEASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPL 301

Query: 519 QPQAFGAFSTASMTTEFVILDEHGIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYF 578
           QPQAFGAFST SMTT  +I DE+G+PYP+DQPC GE+GLFP +LGA++ LLNA+HDEVYF
Sbjct: 302 QPQAFGAFSTPSMTTRIIIFDENGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVYF 361

Query: 579 KGMPIYNGMQLRRHGDIIKRTVGGYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESIL 638
           KGMP+Y   +LRRHGDI+KRTVGGY  VQGRADDTMNLGGIKTSS+EIER+CD  DE I 
Sbjct: 362 KGMPMYKETRLRRHGDIVKRTVGGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECIS 421

Query: 639 ETAAVSLSPIEGGPEQLVILVVLKKQY-VKSPQELKDKFSKAIQKNLNPLFKVGFVKIVP 698
           ETAAV+L+P  GGPE LVI  VLK+ +  +S +ELK KFS+ IQK+LNPLFKV FVKIVP
Sbjct: 422 ETAAVTLTPPNGGPELLVIFAVLKEGFKQQSGEELKMKFSRTIQKDLNPLFKVSFVKIVP 481

Query: 699 SFPRTASNKLLRRVLRKQMKDE-LALRSQL 726
            FPRTAS+KLLRRVLR Q+K E L+LRS++
Sbjct: 482 EFPRTASSKLLRRVLRDQIKQELLSLRSRI 511

BLAST of MELO3C014683.jh1 vs. TAIR 10
Match: AT5G23050.1 (acyl-activating enzyme 17 )

HSP 1 Score: 705.3 bits (1819), Expect = 5.1e-203
Identity = 363/730 (49.73%), Postives = 492/730 (67.40%), Query Frame = 0

Query: 3   KSIWELGVEDLVKTGLNIHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHPFE 62
           KS+  +   D+   G++   + +  R L++++  +  S P E W ++ +RR L P  PF 
Sbjct: 4   KSLNSITKSDIEALGISGDVSEKLLRDLEDIIHGS--STPPETWIQI-SRRILHPNLPFS 63

Query: 63  LHRLLYYSVYANWNISSNGPP-PYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISSF 122
            H+++YY  Y ++     GP  P W P    + LTN+GK++E  G + LG +YK+P+SSF
Sbjct: 64  FHQMMYYGCYKDF-----GPDLPAWIPDPKVASLTNVGKLLEKRGKEFLGGNYKNPVSSF 123

Query: 123 SLFQKLSYQSPEIYWSIVLQELSVTFQKAPRCILDRTAKSN---VRWLPDSILNIAECCL 182
           S FQ+ S  +PE+YW  VL EL++ F   P+CIL++    +    +WLP + LN A  CL
Sbjct: 124 SSFQEFSVSNPEVYWKTVLDELNILFSVPPKCILEKDTSGDNPGGKWLPGAYLNPARNCL 183

Query: 183 LPSSRPLKDDNSLAIVWRDEGCDNSNVNSMTLKQLREKVTLVANALDAT-FSKGDAIAID 242
               +   DD  + I WRDEG D+  VN+MTL +LR +V L A+AL A    +  AIA+D
Sbjct: 184 TNGFKRRLDD--IVIRWRDEGSDDLPVNTMTLLELRSQVWLAAHALSALGLEEESAIAVD 243

Query: 243 MPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPL 302
           MP+ V++V+IYLAIVLAG VVVSIADSF+  E++ RL+ISKAK IFTQD I+RG K  PL
Sbjct: 244 MPMNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKISKAKAIFTQDVIIRGDKSIPL 303

Query: 303 YSRIIEGGSCKAIVIPATGNRLDVDLRKQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVT 362
           Y R+++  +  AIV+PA G+   + LR++DLSW  FL  A+   R   Y          T
Sbjct: 304 YRRVVDAEAPLAIVVPARGSSCRMKLREKDLSWNNFLGNARNL-RGVEYVAAEKPAGAYT 363

Query: 363 NILFSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVLCWPTNLGWVMGPISVYSSL 422
           NILFSSGTTGEPKAIPWT +SP++ AAD+W H+DVQ GDV+ WPTNLGW+MGP  VY+SL
Sbjct: 364 NILFSSGTTGEPKAIPWTNISPLKSAADAWCHLDVQRGDVVAWPTNLGWMMGPWLVYASL 423

Query: 423 LAGATLALYHGSPLGHGFGKFVQDAGVTLLGTVPSLVKTWKDTRCMEGLNWTKIRHFAST 482
           + GA + LY+GSPLG  F KFVQDA V++LG +PS+V+TW+++    G +W++IR F ST
Sbjct: 424 INGACMGLYNGSPLGPTFAKFVQDAEVSVLGVIPSIVRTWQNSNSTSGYDWSRIRCFGST 483

Query: 483 GETSNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVIL 542
           GE SNID+ LWL  +++YKP+ E+CGGTE+G S+I+G+ +QPQ+  AFSTA+M  +  IL
Sbjct: 484 GEASNIDEYLWLMGRAHYKPVIEYCGGTEIGGSFISGSLLQPQSLAAFSTAAMGCKLFIL 543

Query: 543 DEHGIPYPEDQPCIGEIGLFPLYLGASNELLNADHDEVYFKGMPIYNGMQLRRHGDIIKR 602
           DE   P P     +GE+ L P   GAS+ LLN +H +VYF+GMP + G  LRRHGD+ +R
Sbjct: 544 DEDSNPIPPYAAGVGELALCPHMFGASSTLLNGNHFKVYFQGMPTFQGQILRRHGDLFER 603

Query: 603 TVGGYLIVQGRADDTMNLGGIKTSSVEIERICDHFDESILETAAVSLSPIEGGPEQLVIL 662
           T  GY    GRADDTMNLGGIK  S+EIER+C+  D+S+LETAA+ + P  GGPEQLVI 
Sbjct: 604 TSKGYYRAHGRADDTMNLGGIKVGSIEIERVCNSVDDSVLETAAIGVPPPSGGPEQLVIA 663

Query: 663 VVLKKQYVKSP--QELKDKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQM 722
           VV K     +P    LK  F+  IQK LNPLFKV  V  +PS PRTA+NK++RRVLR+Q+
Sbjct: 664 VVFKSPEFSNPDLNLLKKSFNSEIQKKLNPLFKVSSVVTLPSLPRTATNKVMRRVLRQQL 721

Query: 723 KDELALRSQL 726
             +  L S+L
Sbjct: 724 -TQTGLNSKL 721

BLAST of MELO3C014683.jh1 vs. TAIR 10
Match: AT3G48990.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 60.1 bits (144), Expect = 8.6e-09
Identity = 120/522 (22.99%), Postives = 209/522 (40.04%), Query Frame = 0

Query: 207 SMTLKQLREKVTLVANAL--DATFSKGDAIAIDMPLTVDAVVIYLAIVLAGLVVVSIADS 266
           ++T  +L + +   A+ L  DA    GD +A+  P TV+ V+++LA++ A      +  +
Sbjct: 30  NLTHARLHDLIERAASRLVSDAGIKPGDVVALTFPNTVEFVIMFLAVIRARATAAPLNAA 89

Query: 267 FAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSRIIEGGSCKAIVIPATGNRLDVDLR 326
           + A E    L  S +K + T      G       +  ++     A ++ A G+ L + + 
Sbjct: 90  YTAEEFEFYLSDSDSKLLLTSK---EGNAPAQEAASKLKISHVTATLLDA-GSDLVLSVA 149

Query: 327 KQDLSWKEFLSTAKEFPRSNRYSPVYHSRDGVTNILFSSGTTGEPKAIPWTQLSPIRCAA 386
             D      + +A E         V H  DG    L +SGTT  PK +P TQL+      
Sbjct: 150 DSD----SVVDSATEL--------VNHPDDGAL-FLHTSGTTSRPKGVPLTQLNLASSVK 209

Query: 387 DSWAHMDVQAGDVLCWPTNLGWVMGPIS-VYSSLLAGATLAL-YHGSPLGHGFGKFVQDA 446
           +  A   +   D       L  V G ++ + SSL AGA + L   G      F   ++  
Sbjct: 210 NIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLPAAGRFSATTFWPDMKKY 269

Query: 447 GVTLLGTVPSLVKTWKDTRCME-GLNWTKIRHFASTGETSNIDDDLWLSSKSYYKPIFEF 506
             T    VP++ +   D         + K+R F  +   S     L    +++  P+ E 
Sbjct: 270 NATWYTAVPTIHQIILDRHASHPETEYPKLR-FIRSCSASLAPVILSRLEEAFGAPVLEA 329

Query: 507 CGGTELGSSYITGTPVQPQAFGAFSTASMTTEFVILDEHGIPYPEDQPCIGEIGLFPLYL 566
              TE  +  ++  P+  +  G     S+          G P  ++   + E G      
Sbjct: 330 YAMTE-ATHLMSSNPLPEE--GPHKPGSV----------GKPVGQEMAILNEKGEIQEPN 389

Query: 567 GASNELLNADHDEVYFKGMPIYN--GMQLR--RHGDIIKRTVGGYLIVQGRADDTMNLGG 626
                 +   +    +K  P  N  G +      GDI      GYL + GR  + +N GG
Sbjct: 390 NKGEVCIRGPNVTKGYKNNPEANKAGFEFGWFHTGDIGYFDTDGYLHLVGRIKELINRGG 449

Query: 627 IKTSSVEIERI-CDHFDESILETAAVSLSPIEGGPEQLVILVVLKKQYVKSPQELKDKFS 686
            K S +E++ +   H D S  +  A  + P E   E++   V+ ++    + +++K  F 
Sbjct: 450 EKISPIEVDAVLLTHPDVS--QGVAFGV-PDEKYGEEINCAVIPREGTTVTEEDIK-AFC 509

Query: 687 KAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDE 719
           K   KNL        V I  + P+TAS K+ RR++ +   ++
Sbjct: 510 K---KNLAAFKVPKRVFITDNLPKTASGKIQRRIVAQHFLEK 513

BLAST of MELO3C014683.jh1 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 43.9 bits (102), Expect = 6.4e-04
Identity = 104/520 (20.00%), Postives = 201/520 (38.65%), Query Frame = 0

Query: 219 LVANALDATFSKGDAIAIDMPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISK 278
           L A+ L    ++ D ++I  P       ++ ++ + G V+  I     A  +A+ LR ++
Sbjct: 52  LAASLLSLNITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAE 111

Query: 279 AKGIFTQ-DFILRGGKKYPLYSRIIEGGSCKAIVIPATGNRLDVDLR--KQDLSWKEFLS 338
            K +F   +F     +   L          + I+I    N +D   +   ++L ++  + 
Sbjct: 112 PKILFVDYEFAPLIQEVLRLIPTYQSQPHPRIILI----NEIDSTTKPFSKELDYEGLIR 171

Query: 339 TAKEFPRSNRYSPVYHSRDGVTNILFSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAG 398
             +  P S+      H+     ++ ++SGTT +PK +  +       A  S    ++   
Sbjct: 172 KGEPTPSSSASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIF 231

Query: 399 DVLCWPTNLGWVMGPISVYSSLLAGAT-LALYHGSPLGHGFGKFVQDAGVTLLGTVPSLV 458
            V  W   +    G    +S    G T + + H +       K ++  GVT +  VP++ 
Sbjct: 232 PVYLWTLPMFHCNGWTHTWSVAARGGTNVCIRHVT--APEIYKNIELHGVTHMSCVPTVF 291

Query: 459 KTWKDTRCMEGLNWTKIRHFASTGETSNIDDDLWLSSKSYYKPIFEFCGGTELGSSYITG 518
           +       +EG          S  + S     + + +     P        +LG   + G
Sbjct: 292 RF-----LLEG----------SRTDQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHG 351

Query: 519 TPVQPQAFGAFSTASMTTEFVILDEHGIPYPEDQPCIGEIGLFPLYLGASNELLNADHD- 578
             +  +A G         E+  L EH     + +  +  + L  + +  +  L +   D 
Sbjct: 352 YGL-TEATGPVLFCEWQDEWNKLPEHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDG 411

Query: 579 ----EVYFKGMPIYNG--------MQLRRHGDIIKRTVG-----GYLIVQGRADDTMNLG 638
               E+  KG  +  G         +  +HG +    +G     GY+ ++ R+ D +  G
Sbjct: 412 KTMGEIVIKGSSLMKGYLKNPKATSEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIIISG 471

Query: 639 GIKTSSVEIERICDHFDESILETAAVSL-SPIEGGPEQLVILVVLKK---QYVKSPQELK 698
           G   SS+E+E++   + E +LE A V++  P+ G  E     VVLKK     V S  +L 
Sbjct: 472 GENISSIEVEKVLYMYQE-VLEAAVVAMPHPLWG--ETPCAFVVLKKGEEGLVTSEGDL- 531

Query: 699 DKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLLRRVLR 713
               K  ++N+        V      P+ ++ K+L+  LR
Sbjct: 532 ---IKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSKLR 542