MELO3C014520 (gene) Melon (DHL92) v4

BLAST of MELO3C014520 vs. NCBI nr
Match: XP_008449598.1 (PREDICTED: E3 ubiquitin-protein ligase PUB22-like [Cucumis melo])

HSP 1 Score: 809.3 bits (2089), Expect = 1.5e-230
Identity = 414/414 (100.00%), Postives = 414/414 (100.00%), Query Frame = 0

Query: 1   MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT 60
           MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT
Sbjct: 1   MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT 60

Query: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN 120
           KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN
Sbjct: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN 120

Query: 121 SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180
           SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS
Sbjct: 121 SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180

Query: 181 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240
           SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE
Sbjct: 181 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240

Query: 241 TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300
           TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL
Sbjct: 241 TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300

Query: 301 VVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE 360
           VVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE
Sbjct: 301 VVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE 360

Query: 361 MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 415
           MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST
Sbjct: 361 MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 414

BLAST of MELO3C014520 vs. NCBI nr
Match: KAA0061734.1 (E3 ubiquitin-protein ligase PUB22-like [Cucumis melo var. makuwa])

HSP 1 Score: 806.2 bits (2081), Expect = 1.3e-229
Identity = 413/414 (99.76%), Postives = 413/414 (99.76%), Query Frame = 0

Query: 1   MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT 60
           MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT
Sbjct: 1   MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT 60

Query: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN 120
           KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN
Sbjct: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN 120

Query: 121 SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180
           SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS
Sbjct: 121 SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180

Query: 181 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240
           SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE
Sbjct: 181 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240

Query: 241 TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300
           TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL
Sbjct: 241 TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300

Query: 301 VVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE 360
           VVLDQLC CAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE
Sbjct: 301 VVLDQLCECAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE 360

Query: 361 MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 415
           MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST
Sbjct: 361 MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 414

BLAST of MELO3C014520 vs. NCBI nr
Match: TYJ96072.1 (E3 ubiquitin-protein ligase PUB22-like [Cucumis melo var. makuwa])

HSP 1 Score: 805.4 bits (2079), Expect = 2.2e-229
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 0

Query: 3   MEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQ 62
           MEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQ
Sbjct: 1   MEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQ 60

Query: 63  PLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSN 122
           PLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSN
Sbjct: 61  PLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSN 120

Query: 123 HKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQ 182
           HKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQ
Sbjct: 121 HKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQ 180

Query: 183 AHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRETV 242
           AHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRETV
Sbjct: 181 AHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRETV 240

Query: 243 RVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLVV 302
           RVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLVV
Sbjct: 241 RVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLVV 300

Query: 303 LDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEML 362
           LDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEML
Sbjct: 301 LDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEML 360

Query: 363 EVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 415
           EVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST
Sbjct: 361 EVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 412

BLAST of MELO3C014520 vs. NCBI nr
Match: XP_004140266.2 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN48322.2 hypothetical protein Csa_004119 [Cucumis sativus])

HSP 1 Score: 787.7 bits (2033), Expect = 4.8e-224
Identity = 402/414 (97.10%), Postives = 407/414 (98.31%), Query Frame = 0

Query: 1   MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT 60
           MKMED LEENG IEVPSYFLCPISLQLMRDPVTISTGITYDRSSIE WLFSFNKTVCPIT
Sbjct: 1   MKMEDHLEENGEIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEMWLFSFNKTVCPIT 60

Query: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN 120
           KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQ+FP+
Sbjct: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQSFPD 120

Query: 121 SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180
           S HKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS
Sbjct: 121 STHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180

Query: 181 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240
           SQ HLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE
Sbjct: 181 SQTHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240

Query: 241 TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300
            VRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL
Sbjct: 241 IVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300

Query: 301 VVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE 360
           V+LDQLCGCAEGREKLV HAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKV+QE
Sbjct: 301 VILDQLCGCAEGREKLVAHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVMQE 360

Query: 361 MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 415
           MLEVGVVAKLCLVLQMDCS+KTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST
Sbjct: 361 MLEVGVVAKLCLVLQMDCSMKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 414

BLAST of MELO3C014520 vs. NCBI nr
Match: XP_038902057.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 736.1 bits (1899), Expect = 1.7e-208
Identity = 375/414 (90.58%), Postives = 391/414 (94.44%), Query Frame = 0

Query: 1   MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT 60
           MKME++   +GGIEVPSYFLCPISLQLMRDPVTISTGITYDRS+IEKWLFS NK +CPIT
Sbjct: 1   MKMEEE-NNDGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRICPIT 60

Query: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN 120
           KQPLS DPDLLTPNHTLRRLIQSWCTLNAS+GIERIPTPKSPIDKTH+AKILK+AQNFPN
Sbjct: 61  KQPLSPDPDLLTPNHTLRRLIQSWCTLNASHGIERIPTPKSPIDKTHVAKILKEAQNFPN 120

Query: 121 SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180
           SN KCLLALKAI LE+ERNRNLVAQSDG F+FL  VIKSGGSNNI SIE AVEILFHIK 
Sbjct: 121 SNQKCLLALKAIVLESERNRNLVAQSDGVFEFLTKVIKSGGSNNIGSIESAVEILFHIKP 180

Query: 181 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240
           SQ HLKNLVNGD +FINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLM IE+ELL E
Sbjct: 181 SQTHLKNLVNGDSNFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMGIEKELLSE 240

Query: 241 TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300
            VRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAV VLVELLLNS+ERRWSELGL
Sbjct: 241 IVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVQVLVELLLNSSERRWSELGL 300

Query: 301 VVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE 360
           VVLDQLCGCAEGREKLVEH AG+AVVSKKILRVSAMASDRAVRILSSICRFSAS KV+QE
Sbjct: 301 VVLDQLCGCAEGREKLVEHGAGVAVVSKKILRVSAMASDRAVRILSSICRFSASVKVVQE 360

Query: 361 MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 415
           MLEVGVVAKLCLVLQMDCSLKTREKARD LKLHSRVWSNSSCIPPHLLSAYPS+
Sbjct: 361 MLEVGVVAKLCLVLQMDCSLKTREKARDILKLHSRVWSNSSCIPPHLLSAYPSS 413

BLAST of MELO3C014520 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 2.2e-115
Identity = 215/433 (49.65%), Postives = 302/433 (69.75%), Query Frame = 0

Query: 13  IEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQPLSSDPDLLT 72
           IE+PS+FLCPISL +M+DPV +STGITYDR SIEKWLFS  K  CP+TKQ ++     LT
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD--LT 64

Query: 73  PNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSNHKCLLALKAI 132
           PNHTLRRLIQSWCTLNAS GIERIPTPK PI K+ I K++K++ +   +  KCL  L+ I
Sbjct: 65  PNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQI 124

Query: 133 ALENERNR-------------NLVAQSDGAFDFLAMVIKSGGSNNIAS------------ 192
             EN  N+             N+V+ S   ++  +  + S   N++              
Sbjct: 125 VSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSR 184

Query: 193 --IELAVEILFHIKSSQAHLKNLVNG--DVDFINSLTFVLQNGNCQSRAYAVMLLKSSLE 252
             ++ A+ +L+H+ +S+  LK+L+N     + + +LT ++Q G  +SRAYA +LLK  LE
Sbjct: 185 SLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLE 244

Query: 253 LADPIRLMSIERELLRETVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVL 312
           +ADP++++ +EREL  E ++++HD ISH+A++SA+++L   CPWGRNR KAVEGG + ++
Sbjct: 245 VADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISMI 304

Query: 313 VELLLN---STERRWSELGLVVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDR 372
           +ELL++   S+ERR SE+ +VVLD LC CAEGR + + H A +AVVSKKILRVS + S+R
Sbjct: 305 IELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSER 364

Query: 373 AVRILSSICRFSASAKVLQEMLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNS 414
           AVR+L S+ RF A+  +LQEML++GVVAKLCLVLQ+ C  KT+EKA++ LKLH+RVW  S
Sbjct: 365 AVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHARVWRES 424

BLAST of MELO3C014520 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 6.5e-115
Identity = 211/405 (52.10%), Postives = 299/405 (73.83%), Query Frame = 0

Query: 13  IEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQPLSSDPDLLT 72
           IE+P +FLCPISL++M+DPV +STGITYDR SIEKWLF+  K  CP+TKQ + +D D LT
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDI-TDAD-LT 69

Query: 73  PNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSNHKCLLALKAI 132
           PNHTLRRLIQSWCTLNAS G+ERIPTP+ PI K+ I K+++ + +   +  KCL  L+ I
Sbjct: 70  PNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 129

Query: 133 ALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQAHLKNLVNG- 192
             EN  N+  + ++ G  +FLA ++ S  S N +  + A+ +L+H+++S+  LKNL+N  
Sbjct: 130 VSENATNKRCL-EAAGVPEFLANIV-SNDSENGSLTDEALNLLYHLETSETVLKNLLNNK 189

Query: 193 -DVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRETVRVIHDHIS 252
            D + + SLT ++Q G  +SR YA +LLK+ LE+ADP++ M+++ E+  E V+++ D IS
Sbjct: 190 KDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRIS 249

Query: 253 HQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLN---STERRWSELGLVVLDQLC 312
            +A+K+A+ +L  +CPWGRNR KAVE G + V++ELL++   ++ERR  E+ +VVLD LC
Sbjct: 250 QKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLLC 309

Query: 313 GCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEMLEVGVV 372
            CAEGR + + H A +AVV KKILRVS  ASDRAVR+L S+ RF A+  +L EML++GVV
Sbjct: 310 QCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGVV 369

Query: 373 AKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYP 413
           AKLCLVLQ+ C  KT+EKA++ LKLH+RVW +S C+P +++ AYP
Sbjct: 370 AKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of MELO3C014520 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 4.6e-68
Identity = 167/449 (37.19%), Postives = 244/449 (54.34%), Query Frame = 0

Query: 5   DQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQPL 64
           DQ EE   IE+P+YF+CPISL++M+DPVT  +GITYDR +I KWL       CP+TKQPL
Sbjct: 2   DQEEEE--IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWLEKVPS--CPVTKQPL 61

Query: 65  SSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN---S 124
             D D LTPNH LRRLIQ WC  N + G+ RI TP+ P  K ++ + +K  + F      
Sbjct: 62  PLDSD-LTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALG 121

Query: 125 NHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIK-----SGGSNNIASIELAVEILF 184
             + L  L+ +A++   NR L+ +       +  V+K       G   I  ++ ++ +L 
Sbjct: 122 REETLQKLEVLAMDG-NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLH 181

Query: 185 HIKSSQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLE------------- 244
            I       K ++  +   + SLT+VL   +  S+AY ++LL++  E             
Sbjct: 182 LIGIPSNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPE 241

Query: 245 --------LADPIRLMSIERELLRETV--------------RVIHDHISHQASKSALKVL 304
                   L D +  ++     +RETV              ++ H  +  QA  +AL +L
Sbjct: 242 IFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMIL 301

Query: 305 AEVCPWGRNRVKAVEGGAVGVLVELLLNST-ERRWSELGLVVLDQLCGCAEGREKLVEHA 364
            E   W RNR   V+ GAV  L+EL ++ T E+R +EL L VL +LC CA GR +++ H 
Sbjct: 302 LETSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHR 361

Query: 365 AGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEMLEVGVVAKLCLVLQMDCSL 410
            G+AVV+K++LRVS  A DRA+ IL+++ +FS    V++EM+ VG V KLC VL MDC L
Sbjct: 362 GGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGL 421

BLAST of MELO3C014520 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 1.2e-52
Identity = 134/396 (33.84%), Postives = 211/396 (53.28%), Query Frame = 0

Query: 12  GIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQPLSSDPDLL 71
           GI++P +F CPISL+LM+DPVT+ TG TYDR+SIE W+   N T CP+T+ PLS     L
Sbjct: 11  GIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSD--FTL 70

Query: 72  TPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFP------NSNHKC 131
            PNHTLRRLIQ WC  N SNG+ERIPTPK P D T +  +L QA           S    
Sbjct: 71  IPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAA 130

Query: 132 LLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQAHL 191
           L  L+  A ++++NR L+A  +     + ++     S+ + S  LA+ ++  I      +
Sbjct: 131 LRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQFV 190

Query: 192 KNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLK---SSLELADPIRLMSIERELLRETV 251
              ++ D   +  LT +L + + ++R  A  L++   +  + AD    +S    +    +
Sbjct: 191 S--ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVL 250

Query: 252 RVIHDHI-SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLV 311
            ++ + I S +A K  +K L  +C     R  A+  GA  +L++ L    +R  +E  L 
Sbjct: 251 DLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALA 310

Query: 312 VLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEM 371
            ++ LC   EG     EHA  + ++ K ILRVS  A++ A   L ++C  +A  +  +E 
Sbjct: 311 TVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC--TAEERWREEA 370

Query: 372 LEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVW 398
              GVV +L L++Q +C+ + ++KA+  LKL    W
Sbjct: 371 AGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of MELO3C014520 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 3.9e-51
Identity = 138/407 (33.91%), Postives = 215/407 (52.83%), Query Frame = 0

Query: 12  GIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQPLSSDPDLL 71
           GI++P +F CPISL LM DPVTISTG TYDR+SI+ W+ +   T CP+T+  LS     L
Sbjct: 11  GIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSD--FTL 70

Query: 72  TPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFP------NSNHKC 131
            PNHTLRRLIQ WC  N SNG+ERIPTPK P D   +  +L QA           S    
Sbjct: 71  IPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAA 130

Query: 132 LLALKAIALENERNRNLVAQSDGAFDFLAMVI-----KSGGSNNIASIELAVEILFHIKS 191
           +  L+ +A ++E+NR L+A    A + L  ++      +  S+ + S  LA+ +L H+  
Sbjct: 131 IRRLRGLARDSEKNRVLIA-GHNAREILVRILFADIETTSLSSELVSESLALLVLLHMTE 190

Query: 192 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRL---MSIEREL 251
           ++      V  D   +  +T +L + + + R  A  L++  L  A  + L   +S    +
Sbjct: 191 TEC---EAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSI 250

Query: 252 LRETVRVIHDHI-SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWS 311
               + ++ + I S +A K  +K +  +C   + R  A+  GA G+L++ L    +R  +
Sbjct: 251 FEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDT 310

Query: 312 ELGLVVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAK 371
           E GL  ++ LC   EG     EHA  + ++ K ILRVS  A++ A   L ++C  +A  +
Sbjct: 311 ERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC--TAEER 370

Query: 372 VLQEMLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCI 404
              E    G+V +L L++Q DC+ + + KA+  LKL    W + S +
Sbjct: 371 CRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTV 408

BLAST of MELO3C014520 vs. ExPASy TrEMBL
Match: A0A1S3BME5 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491434 PE=4 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 7.5e-231
Identity = 414/414 (100.00%), Postives = 414/414 (100.00%), Query Frame = 0

Query: 1   MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT 60
           MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT
Sbjct: 1   MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT 60

Query: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN 120
           KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN
Sbjct: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN 120

Query: 121 SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180
           SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS
Sbjct: 121 SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180

Query: 181 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240
           SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE
Sbjct: 181 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240

Query: 241 TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300
           TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL
Sbjct: 241 TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300

Query: 301 VVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE 360
           VVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE
Sbjct: 301 VVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE 360

Query: 361 MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 415
           MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST
Sbjct: 361 MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 414

BLAST of MELO3C014520 vs. ExPASy TrEMBL
Match: A0A5A7V391 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold212G001090 PE=4 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 6.3e-230
Identity = 413/414 (99.76%), Postives = 413/414 (99.76%), Query Frame = 0

Query: 1   MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT 60
           MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT
Sbjct: 1   MKMEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPIT 60

Query: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN 120
           KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN
Sbjct: 61  KQPLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN 120

Query: 121 SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180
           SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS
Sbjct: 121 SNHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKS 180

Query: 181 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240
           SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE
Sbjct: 181 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE 240

Query: 241 TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300
           TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL
Sbjct: 241 TVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGL 300

Query: 301 VVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE 360
           VVLDQLC CAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE
Sbjct: 301 VVLDQLCECAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQE 360

Query: 361 MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 415
           MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST
Sbjct: 361 MLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 414

BLAST of MELO3C014520 vs. ExPASy TrEMBL
Match: A0A5D3B896 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00310 PE=4 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.1e-229
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 0

Query: 3   MEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQ 62
           MEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQ
Sbjct: 1   MEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQ 60

Query: 63  PLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSN 122
           PLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSN
Sbjct: 61  PLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSN 120

Query: 123 HKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQ 182
           HKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQ
Sbjct: 121 HKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQ 180

Query: 183 AHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRETV 242
           AHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRETV
Sbjct: 181 AHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRETV 240

Query: 243 RVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLVV 302
           RVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLVV
Sbjct: 241 RVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLVV 300

Query: 303 LDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEML 362
           LDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEML
Sbjct: 301 LDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEML 360

Query: 363 EVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 415
           EVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST
Sbjct: 361 EVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 412

BLAST of MELO3C014520 vs. ExPASy TrEMBL
Match: A0A0A0KJY2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G426370 PE=4 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 3.4e-223
Identity = 400/412 (97.09%), Postives = 405/412 (98.30%), Query Frame = 0

Query: 3   MEDQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQ 62
           MED LEENG IEVPSYFLCPISLQLMRDPVTISTGITYDRSSIE WLFSFNKTVCPITKQ
Sbjct: 1   MEDHLEENGEIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEMWLFSFNKTVCPITKQ 60

Query: 63  PLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSN 122
           PLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQ+FP+S 
Sbjct: 61  PLSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQSFPDST 120

Query: 123 HKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQ 182
           HKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQ
Sbjct: 121 HKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQ 180

Query: 183 AHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRETV 242
            HLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRE V
Sbjct: 181 THLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLREIV 240

Query: 243 RVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLVV 302
           RVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLV+
Sbjct: 241 RVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLVI 300

Query: 303 LDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEML 362
           LDQLCGCAEGREKLV HAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKV+QEML
Sbjct: 301 LDQLCGCAEGREKLVAHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVMQEML 360

Query: 363 EVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 415
           EVGVVAKLCLVLQMDCS+KTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST
Sbjct: 361 EVGVVAKLCLVLQMDCSMKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 412

BLAST of MELO3C014520 vs. ExPASy TrEMBL
Match: A0A6J1FYK9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448788 PE=4 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 2.3e-203
Identity = 361/411 (87.83%), Postives = 391/411 (95.13%), Query Frame = 0

Query: 6   QLEENGG--IEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQP 65
           ++EE+GG  +EVPSYFLCPISLQLMRDPVTISTGITYDRS+IEKWLFS NK  CPITKQP
Sbjct: 2   RMEEDGGGDLEVPSYFLCPISLQLMRDPVTISTGITYDRSNIEKWLFSCNKRTCPITKQP 61

Query: 66  LSSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSNH 125
           L SDPDL+TPNHTLRRLIQ+WCTLNAS+G+ERIPTPKSPIDKTH+AKILKQA+NFPNSNH
Sbjct: 62  L-SDPDLVTPNHTLRRLIQAWCTLNASHGVERIPTPKSPIDKTHVAKILKQAKNFPNSNH 121

Query: 126 KCLLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQA 185
           KCLLALKAIALE+ERN  LVAQSDGAF+F+A VIKSGG  NI S+ELAVEILFHIKSS+A
Sbjct: 122 KCLLALKAIALESERNCTLVAQSDGAFEFVAAVIKSGGDKNIGSVELAVEILFHIKSSEA 181

Query: 186 HLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRETVR 245
           HLKNLVNGD +FINSLTFVL+NGNCQSRAYA+MLLKSSLELADPIRLMSIEREL+ E VR
Sbjct: 182 HLKNLVNGDGNFINSLTFVLKNGNCQSRAYAIMLLKSSLELADPIRLMSIERELVSEIVR 241

Query: 246 VIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLVVL 305
           VIHDHISHQASKSALK+LAEVCPWGRNRVKAVEGGAVGVLVELLLNST+RRWSE+GLV+L
Sbjct: 242 VIHDHISHQASKSALKILAEVCPWGRNRVKAVEGGAVGVLVELLLNSTDRRWSEIGLVIL 301

Query: 306 DQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEMLE 365
           DQLCGCAEGREKL+EH AG+A+VSKKILRVSAMASDRAVRILSSICRFSAS K++QEMLE
Sbjct: 302 DQLCGCAEGREKLMEHGAGVAIVSKKILRVSAMASDRAVRILSSICRFSASKKIVQEMLE 361

Query: 366 VGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYPST 415
           VGVVAKLCLVLQMDCS KTREKARD LKLHSRVWSNSSCIPPHL SAYPS+
Sbjct: 362 VGVVAKLCLVLQMDCSSKTREKARDILKLHSRVWSNSSCIPPHLASAYPSS 411

BLAST of MELO3C014520 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 417.2 bits (1071), Expect = 1.6e-116
Identity = 215/433 (49.65%), Postives = 302/433 (69.75%), Query Frame = 0

Query: 13  IEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQPLSSDPDLLT 72
           IE+PS+FLCPISL +M+DPV +STGITYDR SIEKWLFS  K  CP+TKQ ++     LT
Sbjct: 5   IEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETD--LT 64

Query: 73  PNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSNHKCLLALKAI 132
           PNHTLRRLIQSWCTLNAS GIERIPTPK PI K+ I K++K++ +   +  KCL  L+ I
Sbjct: 65  PNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQI 124

Query: 133 ALENERNR-------------NLVAQSDGAFDFLAMVIKSGGSNNIAS------------ 192
             EN  N+             N+V+ S   ++  +  + S   N++              
Sbjct: 125 VSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSR 184

Query: 193 --IELAVEILFHIKSSQAHLKNLVNG--DVDFINSLTFVLQNGNCQSRAYAVMLLKSSLE 252
             ++ A+ +L+H+ +S+  LK+L+N     + + +LT ++Q G  +SRAYA +LLK  LE
Sbjct: 185 SLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLE 244

Query: 253 LADPIRLMSIERELLRETVRVIHDHISHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVL 312
           +ADP++++ +EREL  E ++++HD ISH+A++SA+++L   CPWGRNR KAVEGG + ++
Sbjct: 245 VADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISMI 304

Query: 313 VELLLN---STERRWSELGLVVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDR 372
           +ELL++   S+ERR SE+ +VVLD LC CAEGR + + H A +AVVSKKILRVS + S+R
Sbjct: 305 IELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSER 364

Query: 373 AVRILSSICRFSASAKVLQEMLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNS 414
           AVR+L S+ RF A+  +LQEML++GVVAKLCLVLQ+ C  KT+EKA++ LKLH+RVW  S
Sbjct: 365 AVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHARVWRES 424

BLAST of MELO3C014520 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 415.6 bits (1067), Expect = 4.6e-116
Identity = 211/405 (52.10%), Postives = 299/405 (73.83%), Query Frame = 0

Query: 13  IEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQPLSSDPDLLT 72
           IE+P +FLCPISL++M+DPV +STGITYDR SIEKWLF+  K  CP+TKQ + +D D LT
Sbjct: 10  IEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDI-TDAD-LT 69

Query: 73  PNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPNSNHKCLLALKAI 132
           PNHTLRRLIQSWCTLNAS G+ERIPTP+ PI K+ I K+++ + +   +  KCL  L+ I
Sbjct: 70  PNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 129

Query: 133 ALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQAHLKNLVNG- 192
             EN  N+  + ++ G  +FLA ++ S  S N +  + A+ +L+H+++S+  LKNL+N  
Sbjct: 130 VSENATNKRCL-EAAGVPEFLANIV-SNDSENGSLTDEALNLLYHLETSETVLKNLLNNK 189

Query: 193 -DVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRLMSIERELLRETVRVIHDHIS 252
            D + + SLT ++Q G  +SR YA +LLK+ LE+ADP++ M+++ E+  E V+++ D IS
Sbjct: 190 KDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRIS 249

Query: 253 HQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLN---STERRWSELGLVVLDQLC 312
            +A+K+A+ +L  +CPWGRNR KAVE G + V++ELL++   ++ERR  E+ +VVLD LC
Sbjct: 250 QKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLLC 309

Query: 313 GCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEMLEVGVV 372
            CAEGR + + H A +AVV KKILRVS  ASDRAVR+L S+ RF A+  +L EML++GVV
Sbjct: 310 QCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGVV 369

Query: 373 AKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCIPPHLLSAYP 413
           AKLCLVLQ+ C  KT+EKA++ LKLH+RVW +S C+P +++ AYP
Sbjct: 370 AKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of MELO3C014520 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 260.0 bits (663), Expect = 3.2e-69
Identity = 167/449 (37.19%), Postives = 244/449 (54.34%), Query Frame = 0

Query: 5   DQLEENGGIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQPL 64
           DQ EE   IE+P+YF+CPISL++M+DPVT  +GITYDR +I KWL       CP+TKQPL
Sbjct: 16  DQEEEE--IEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWLEKVPS--CPVTKQPL 75

Query: 65  SSDPDLLTPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFPN---S 124
             D D LTPNH LRRLIQ WC  N + G+ RI TP+ P  K ++ + +K  + F      
Sbjct: 76  PLDSD-LTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALG 135

Query: 125 NHKCLLALKAIALENERNRNLVAQSDGAFDFLAMVIK-----SGGSNNIASIELAVEILF 184
             + L  L+ +A++   NR L+ +       +  V+K       G   I  ++ ++ +L 
Sbjct: 136 REETLQKLEVLAMDG-NNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLH 195

Query: 185 HIKSSQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLE------------- 244
            I       K ++  +   + SLT+VL   +  S+AY ++LL++  E             
Sbjct: 196 LIGIPSNDAKTILMENDRVMESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPE 255

Query: 245 --------LADPIRLMSIERELLRETV--------------RVIHDHISHQASKSALKVL 304
                   L D +  ++     +RETV              ++ H  +  QA  +AL +L
Sbjct: 256 IFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMIL 315

Query: 305 AEVCPWGRNRVKAVEGGAVGVLVELLLNST-ERRWSELGLVVLDQLCGCAEGREKLVEHA 364
            E   W RNR   V+ GAV  L+EL ++ T E+R +EL L VL +LC CA GR +++ H 
Sbjct: 316 LETSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHR 375

Query: 365 AGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEMLEVGVVAKLCLVLQMDCSL 410
            G+AVV+K++LRVS  A DRA+ IL+++ +FS    V++EM+ VG V KLC VL MDC L
Sbjct: 376 GGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGL 435

BLAST of MELO3C014520 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 208.8 bits (530), Expect = 8.6e-54
Identity = 134/396 (33.84%), Postives = 211/396 (53.28%), Query Frame = 0

Query: 12  GIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQPLSSDPDLL 71
           GI++P +F CPISL+LM+DPVT+ TG TYDR+SIE W+   N T CP+T+ PLS     L
Sbjct: 11  GIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSD--FTL 70

Query: 72  TPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFP------NSNHKC 131
            PNHTLRRLIQ WC  N SNG+ERIPTPK P D T +  +L QA           S    
Sbjct: 71  IPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAA 130

Query: 132 LLALKAIALENERNRNLVAQSDGAFDFLAMVIKSGGSNNIASIELAVEILFHIKSSQAHL 191
           L  L+  A ++++NR L+A  +     + ++     S+ + S  LA+ ++  I      +
Sbjct: 131 LRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQFV 190

Query: 192 KNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLK---SSLELADPIRLMSIERELLRETV 251
              ++ D   +  LT +L + + ++R  A  L++   +  + AD    +S    +    +
Sbjct: 191 S--ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVL 250

Query: 252 RVIHDHI-SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWSELGLV 311
            ++ + I S +A K  +K L  +C     R  A+  GA  +L++ L    +R  +E  L 
Sbjct: 251 DLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALA 310

Query: 312 VLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAKVLQEM 371
            ++ LC   EG     EHA  + ++ K ILRVS  A++ A   L ++C  +A  +  +E 
Sbjct: 311 TVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC--TAEERWREEA 370

Query: 372 LEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVW 398
              GVV +L L++Q +C+ + ++KA+  LKL    W
Sbjct: 371 AGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of MELO3C014520 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 203.8 bits (517), Expect = 2.8e-52
Identity = 138/407 (33.91%), Postives = 215/407 (52.83%), Query Frame = 0

Query: 12  GIEVPSYFLCPISLQLMRDPVTISTGITYDRSSIEKWLFSFNKTVCPITKQPLSSDPDLL 71
           GI++P +F CPISL LM DPVTISTG TYDR+SI+ W+ +   T CP+T+  LS     L
Sbjct: 11  GIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSD--FTL 70

Query: 72  TPNHTLRRLIQSWCTLNASNGIERIPTPKSPIDKTHIAKILKQAQNFP------NSNHKC 131
            PNHTLRRLIQ WC  N SNG+ERIPTPK P D   +  +L QA           S    
Sbjct: 71  IPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAA 130

Query: 132 LLALKAIALENERNRNLVAQSDGAFDFLAMVI-----KSGGSNNIASIELAVEILFHIKS 191
           +  L+ +A ++E+NR L+A    A + L  ++      +  S+ + S  LA+ +L H+  
Sbjct: 131 IRRLRGLARDSEKNRVLIA-GHNAREILVRILFADIETTSLSSELVSESLALLVLLHMTE 190

Query: 192 SQAHLKNLVNGDVDFINSLTFVLQNGNCQSRAYAVMLLKSSLELADPIRL---MSIEREL 251
           ++      V  D   +  +T +L + + + R  A  L++  L  A  + L   +S    +
Sbjct: 191 TEC---EAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSI 250

Query: 252 LRETVRVIHDHI-SHQASKSALKVLAEVCPWGRNRVKAVEGGAVGVLVELLLNSTERRWS 311
               + ++ + I S +A K  +K +  +C   + R  A+  GA G+L++ L    +R  +
Sbjct: 251 FEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDT 310

Query: 312 ELGLVVLDQLCGCAEGREKLVEHAAGLAVVSKKILRVSAMASDRAVRILSSICRFSASAK 371
           E GL  ++ LC   EG     EHA  + ++ K ILRVS  A++ A   L ++C  +A  +
Sbjct: 311 ERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC--TAEER 370

Query: 372 VLQEMLEVGVVAKLCLVLQMDCSLKTREKARDTLKLHSRVWSNSSCI 404
              E    G+V +L L++Q DC+ + + KA+  LKL    W + S +
Sbjct: 371 CRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPDDSTV 408