MELO3C014272 (gene) Melon (DHL92) v4

Overview
NameMELO3C014272
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionPyruvate kinase
Locationchr05: 4628930 .. 4633843 (+)
RNA-Seq ExpressionMELO3C014272
SyntenyMELO3C014272
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGAAGGAAAGGGGTAACTTTCTTATTTATAAAAATAAATTAAACGTCAATGTTATTAAAAAGATTTTGCTTTTTCGAGTGCAAATTCTCTTGTTTAAAACCCAAACAAACCTCATCCGATCCTTTCTAAGTACAAAAGGTTTAAGCCGTTTTGGGCCGTCGATCTTCCGGCGACAATTCCGAAGCTGTTCTCTTCTTCGGCATCTCCGTCTCTCTCCCTGCATTAATCACCGATCTCCGTTTCCGAAATTCCTCCATTCCCTTTCTCCAAATTCCTCCATTTCCCTTTACAATGACACAATCTCTCCAACTTTTCACTTCCTCCGCCATTTCTCTCCCCAAACACTCCATCTCTAAACCCTTCTCTTCCACTGCCGCTTTCCGCTTTCCTTTCTCCTTCTCCAAATCCTCAATTAGAGCTTCTTCTTCGGATCTCAATCCTCTCTCATCTTCTTCCACTTCCCAAGTCTTGGTTTCTGAAAATGGCTCCAGTTCCGGTGGTGGGGTTGTGTCCTCTTCTGCTACTAGGGAGTTTGTGTCTGTTGCCTCTGATTCCACCTCGATTGATGTTGATGCTGTCACTGAGGCTGAGTTGAAGGAGAATGGGTTCAGGAGTACCAGGAGGACTAAGCTCGTGTGCACCATTGGCCCTGCTAGTTGTGGATTCGAACAGCTTGAGGCACTCGCTGTTGGTGGTATGAATGTCGCTAGGATCAATATGTGCCATGGGACTCGAGACTGGCATCGGACCGTTATTGAACGTGTGCGGAGGCTCAATGATGAGAAGGGTTATGCTGTTGCTATTATGATGGATACCGAAGGGAGTGAAATTCACATGGGTGATCTTGGTGGAGCTTCCTCGGCTAAAGCGGAAGTGAGTTTTTCGATTCTTTTACCGTTTTTTTAATCTTATTGTCTCATAATCAGTCGAATTCTTGATAACATTTGCCCCAGGTTCAAAAATTTTCTGATTGCATTGGGTACTGGGTTTTGTCTAGCTGAGAAACGTTTCTTCTGCCTGAAAGTCTATAATCTGCCAGATTTTTAGATTCTTAAATTGGATCGGAGTTCACTTTCCTTGAAACCTAGTTTTCATAAAGATATTACCCATTTTGTGTTCTAAAACCAAGATGGGTCCTTTCATCATTAAGCAAGTCGTGAACTTTTGTCAAAGGTCTTAAATATATTGTTATCATAACCTACGTGTCACGATATCTTGGCTCAATATATAAACTTTGATCACTGGATTAGTGAAAATGAGTTTACTTACTGTGCAGCTGTAAGTATTCTCGAAGTGGTACTCAGTGAGTCAGTGTTTTATAAAGCTCTCCCAGCACATGCCTAAGCGTTAGGCGTAAGTCTGGCGCTTCTTCTTGGAAAGGCAAGCTTACGAAATTAGGCGCGTCTTCTATGAAGTCCCAAGGTTTAAAAGGTTCAAAGCCCTCGGTCTTGGAACATTTTCATTATTTTTATAATATAGTAATAATGAAGGGTTCTTTTTTTCTTTATTAACTATAAAAAATTAAATTTACTAATTTTACATGCAAAGTTTCTTGTCCGAAGGGATTTTTTTCTTCTTCCAAATTTCCACTTAATACTCAAAAGTGATATATTGCAACTTGAGCTTTTAACATTTGTGATACTTGTCAATTAGCATAGTTCTTCTAATTCGAAGTAAGGAAGATGGTCCCTTCATATCCTGTTATGTTGCTGCTATCTGACTCCGGTTTAGATAAGGCGATGGGGAAGTCGTTGGGTGGGACATGTTTAACTGCTTGCTCTGTTCTATGTAGGATGGTGAAATTTGGACATTCAGTGTCAGAGCTTTTGATTCAACCCTCCCAGAACGCACCATTAATGTGAACTATGAAGGGTTTGCAGAAGGTTACTGTTTCTATCCTAAATAATAACATTTACTGAATGCATGCACAATTTAATACAGCCTTTGGGAATGGCGTGTGTGGATTGGATGTATCTGTGTTCATTGTTGTAGTAAGTTCCATAATATTGTTATTGATCGTGAATTATCATTGATGTTGTGTAGATGTGAGAGTGGGTGATGACCTTCTTGTTGATGGTGGAATGGTGAGGTTTGAGGTAATCGAAAAAATTGGTCCAGATGTTAAATGCCTGTGTACTGACCCGGGATTGTTGTTGCCGCGGGCTAATTTGACTTTTTGGAGGGATGGACATCTAGTAAGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGTAGGTGCCTCTCCTTCCTTAAAATGTCATTTCCTTGGTTAAATTGAATTCATTTGTTGTACGATACCTATTAAAGTACTGATGTACTACTAAATTTACCGTAGCTAACTTAAGCTTTTGGATTCATAGTTTAATTTCGCGTGATTTTTGATTCGGGTGTTCTGTTCTGTGTTCGAATCTCTTTAATGTCATTTCCTCCCCATTAAGACTGATAACCAACTTATTGGGCTTTTTGCTAATATCTTTAACTTTGCAAGTACAGGGTTAAAGTATTATTATAATATTGAATTTTCCATAATTTATGAGCTTAAGATCTTAGGTTAAGTAGTGATTTTACAATTACTAATAATTGTTGTTATGAGCGACTGTAGAAAGAGTTTCAGACTGCGAAAAGAGTTCCATCATCTAGGCTTGCCATATTTTTTTGAACCTATGTAATATGTATAGCTAATTTTAGATGACATTGTTTTAGAACTTGCAGACTTTATACTATTTTATTTTTGTAAGATATCTTAGTGTCAGAAACTTTACCCTAGAATTAGAAAGCTTTTGCTCTCTTATCAAAAACTAACACTTTTGTTTCATTAGCTTAGATATTCAAACTTTCGATTTTGGCATTGCAACCAACTCACACTAAACTTTGTAGAGTAATTAGATTTATGTTGAAAGATTATGTAGAGATAATTATAATTTGGACCATAAATCTTAGGACACGTTTTATGGGGTGAGTAGTTGTATATTCATACTGCGAACTCTTTATGCAACCTAATGATTGTTCCTGGATCAAAGCCTCCTCATATTCTAGGTTAACCTTTTGTGCAACCTTATGGTACAAATTGCCCTGTTACAGTGGCTTCTTTTCTTGTCCGAATTTCTAATGCAAGTTTTACAAATCCAGGATTGGTTGGATATTGATTTTGGGATTGCTGAAGGTGTTGATTTTCTTGCCATATCATTTGTCAAGTCTGCTGAAGTAATTAAACATCTCAAAAGCTATATTGCTGCACGTTCTCGTGGCAGGTGAGCAGAATTATGGTATCGTGCTGATTTGCTCTTTTACTGCATTTGGGTCTGAAGCTTAGGGGACCTTTCATTGTTTGTTCTCACGGATTTAGAGGGATCAAATGTTTCTCAACTATGTGTTAGTATGTTTTGATTCGTAAGTTAGGTGCTGACAGGCTAGTCGATGACAGTCAAATTTTCTCGCCAATTCTGTGGATTTCATCACTAAAATGTTCATATATGCGTCTCCTAAATATTTGTGTTTCTAATTTCACATGCAGTGACATTTCCATCATTGCAAAAATAGAGAGTCTGGACTCGTTGAAGAACTTGGAAGAAATTATTTTAGCATCAGATGGAGCTATGGTAGCTAGAGGAGATTTAGGAGCTCAAATACCACTGGAACAGGTGCCATCAGTCCAGCAAAAGGTCGTCCAACTGTGCAGACAATTAAATAAACCAGTTATTGTTGCTTCTCAGCTGCTTGAGTCAATGATCGAATATCCTACACCCACCAGAGCTGAAGTGGCTGATGTTTCTGAGGCGGTTAGGCAGCGATCAGATGCTCTAATGCTGTCTGGTGAGTCCGCCATGGGCCAGTACCCTGACAAGGCATTGGCTGTTTTGAGAAGTGTCAGTCTAAGAATTGAAAAGTGGTGGAGGGATGAGAAACGCCATGAACCTATGGAACTCCCAGAAGTTGGATCTTCATTCTCAGATAGTATCTTAGAAGAGATCTGCAATTCGGCTGCTAAAATGGGTAAGCCTAAGCTTTATGACTCCTTTGTCCTGTTATAGACAATATACCTATAGAACCATAATTTAGATCTATGTTTTAGAACTATGAACTCAAATAATCTCATATCGTTTCACAAAACTCAAAAGTGGTCATTAGGCCGTATAATTATGGTTTCTTGAGTTCTTTTCCTATTTGTTTACTACAAATCCAGTCTAATTGTGAATTTTGTTTTTGTAGCCAATAATTTGGAGGTAGATGCCATTTTCGTCTACACAACGTCAGGCCACATGGCATCTCTTCTGTCCCGTTGTCGACCTGATTGCCCAATCTTTGCTTTTACTTCCACAACATCTGTTAGGAGACGTCTTAACTTGCAATGGGGATTGATACCCTTCAGACTGAGCTTCTCCGACGACATGGAAAACAACCTCAACAAAACATTTTTGCTGCTTAAAGCCAGAAACTTGATCAAATCAGGTGACCTTGTAATTGCTGTCTCAGACATGTTGCAATCTATCCAAGTTATGAATGTTCCGTAAGGAAGAAGGGGCTCAGTATGGTTCCTGCTGATGTTCCATTCCTTTAAATTATTTAACATAATCAAAGTTTTCTGGATCTGCTATTTAGTTGCTAGGAAAATCGATGGAAACAAGCTTCAGGTTTTTAGTTTGGTTGAACTCTGATTTGCTTGCTTTATTTCTTTCTTATTATGTAACATTTTAGTGGCTATCAGATAAGTGTTCTATGAACAAACTTGGTTTACGAGCTATTTATGTAGTTTTTGTGATAACAATAAGTAATATTGTGTATTTCTGGGCATTCTGTTGAAAAATTGAAGCATCTGGGAGGTCGCCTAAAGTGTTATCATCAAGAACATTTAGGTAGGTTTGTTTATCTATCTTATCTTGCTCATAGACTTGTTTGTTTTTATAAG

mRNA sequence

AAAGAAGGAAAGGGGTAACTTTCTTATTTATAAAAATAAATTAAACGTCAATGTTATTAAAAAGATTTTGCTTTTTCGAGTGCAAATTCTCTTGTTTAAAACCCAAACAAACCTCATCCGATCCTTTCTAAGTACAAAAGGTTTAAGCCGTTTTGGGCCGTCGATCTTCCGGCGACAATTCCGAAGCTGTTCTCTTCTTCGGCATCTCCGTCTCTCTCCCTGCATTAATCACCGATCTCCGTTTCCGAAATTCCTCCATTCCCTTTCTCCAAATTCCTCCATTTCCCTTTACAATGACACAATCTCTCCAACTTTTCACTTCCTCCGCCATTTCTCTCCCCAAACACTCCATCTCTAAACCCTTCTCTTCCACTGCCGCTTTCCGCTTTCCTTTCTCCTTCTCCAAATCCTCAATTAGAGCTTCTTCTTCGGATCTCAATCCTCTCTCATCTTCTTCCACTTCCCAAGTCTTGGTTTCTGAAAATGGCTCCAGTTCCGGTGGTGGGGTTGTGTCCTCTTCTGCTACTAGGGAGTTTGTGTCTGTTGCCTCTGATTCCACCTCGATTGATGTTGATGCTGTCACTGAGGCTGAGTTGAAGGAGAATGGGTTCAGGAGTACCAGGAGGACTAAGCTCGTGTGCACCATTGGCCCTGCTAGTTGTGGATTCGAACAGCTTGAGGCACTCGCTGTTGGTGGTATGAATGTCGCTAGGATCAATATGTGCCATGGGACTCGAGACTGGCATCGGACCGTTATTGAACGTGTGCGGAGGCTCAATGATGAGAAGGGTTATGCTGTTGCTATTATGATGGATACCGAAGGGAGTGAAATTCACATGGGTGATCTTGGTGGAGCTTCCTCGGCTAAAGCGGAAGATGGTGAAATTTGGACATTCAGTGTCAGAGCTTTTGATTCAACCCTCCCAGAACGCACCATTAATGTGAACTATGAAGGGTTTGCAGAAGATGTGAGAGTGGGTGATGACCTTCTTGTTGATGGTGGAATGGTGAGGTTTGAGGTAATCGAAAAAATTGGTCCAGATGTTAAATGCCTGTGTACTGACCCGGGATTGTTGTTGCCGCGGGCTAATTTGACTTTTTGGAGGGATGGACATCTAGTAAGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGATTGGTTGGATATTGATTTTGGGATTGCTGAAGGTGTTGATTTTCTTGCCATATCATTTGTCAAGTCTGCTGAAGTAATTAAACATCTCAAAAGCTATATTGCTGCACGTTCTCGTGGCAGTGACATTTCCATCATTGCAAAAATAGAGAGTCTGGACTCGTTGAAGAACTTGGAAGAAATTATTTTAGCATCAGATGGAGCTATGGTAGCTAGAGGAGATTTAGGAGCTCAAATACCACTGGAACAGGTGCCATCAGTCCAGCAAAAGGTCGTCCAACTGTGCAGACAATTAAATAAACCAGTTATTGTTGCTTCTCAGCTGCTTGAGTCAATGATCGAATATCCTACACCCACCAGAGCTGAAGTGGCTGATGTTTCTGAGGCGGTTAGGCAGCGATCAGATGCTCTAATGCTGTCTGGTGAGTCCGCCATGGGCCAGTACCCTGACAAGGCATTGGCTGTTTTGAGAAGTGTCAGTCTAAGAATTGAAAAGTGGTGGAGGGATGAGAAACGCCATGAACCTATGGAACTCCCAGAAGTTGGATCTTCATTCTCAGATAGTATCTTAGAAGAGATCTGCAATTCGGCTGCTAAAATGGCCAATAATTTGGAGGTAGATGCCATTTTCGTCTACACAACGTCAGGCCACATGGCATCTCTTCTGTCCCGTTGTCGACCTGATTGCCCAATCTTTGCTTTTACTTCCACAACATCTGTTAGGAGACGTCTTAACTTGCAATGGGGATTGATACCCTTCAGACTGAGCTTCTCCGACGACATGGAAAACAACCTCAACAAAACATTTTTGCTGCTTAAAGCCAGAAACTTGATCAAATCAGGTGACCTTGTAATTGCTGTCTCAGACATGTTGCAATCTATCCAAGTTATGAATGTTCCGTAAGGAAGAAGGGGCTCAGTATGGTTCCTGCTGATGTTCCATTCCTTTAAATTATTTAACATAATCAAAGTTTTCTGGATCTGCTATTTAGTTGCTAGGAAAATCGATGGAAACAAGCTTCAGGTTTTTAGTTTGGTTGAACTCTGATTTGCTTGCTTTATTTCTTTCTTATTATGTAACATTTTAGTGGCTATCAGATAAGTGTTCTATGAACAAACTTGGTTTACGAGCTATTTATGTAGTTTTTGTGATAACAATAAGTAATATTGTGTATTTCTGGGCATTCTGTTGAAAAATTGAAGCATCTGGGAGGTCGCCTAAAGTGTTATCATCAAGAACATTTAGGTAGGTTTGTTTATCTATCTTATCTTGCTCATAGACTTGTTTGTTTTTATAAG

Coding sequence (CDS)

ATGACACAATCTCTCCAACTTTTCACTTCCTCCGCCATTTCTCTCCCCAAACACTCCATCTCTAAACCCTTCTCTTCCACTGCCGCTTTCCGCTTTCCTTTCTCCTTCTCCAAATCCTCAATTAGAGCTTCTTCTTCGGATCTCAATCCTCTCTCATCTTCTTCCACTTCCCAAGTCTTGGTTTCTGAAAATGGCTCCAGTTCCGGTGGTGGGGTTGTGTCCTCTTCTGCTACTAGGGAGTTTGTGTCTGTTGCCTCTGATTCCACCTCGATTGATGTTGATGCTGTCACTGAGGCTGAGTTGAAGGAGAATGGGTTCAGGAGTACCAGGAGGACTAAGCTCGTGTGCACCATTGGCCCTGCTAGTTGTGGATTCGAACAGCTTGAGGCACTCGCTGTTGGTGGTATGAATGTCGCTAGGATCAATATGTGCCATGGGACTCGAGACTGGCATCGGACCGTTATTGAACGTGTGCGGAGGCTCAATGATGAGAAGGGTTATGCTGTTGCTATTATGATGGATACCGAAGGGAGTGAAATTCACATGGGTGATCTTGGTGGAGCTTCCTCGGCTAAAGCGGAAGATGGTGAAATTTGGACATTCAGTGTCAGAGCTTTTGATTCAACCCTCCCAGAACGCACCATTAATGTGAACTATGAAGGGTTTGCAGAAGATGTGAGAGTGGGTGATGACCTTCTTGTTGATGGTGGAATGGTGAGGTTTGAGGTAATCGAAAAAATTGGTCCAGATGTTAAATGCCTGTGTACTGACCCGGGATTGTTGTTGCCGCGGGCTAATTTGACTTTTTGGAGGGATGGACATCTAGTAAGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGATTGGTTGGATATTGATTTTGGGATTGCTGAAGGTGTTGATTTTCTTGCCATATCATTTGTCAAGTCTGCTGAAGTAATTAAACATCTCAAAAGCTATATTGCTGCACGTTCTCGTGGCAGTGACATTTCCATCATTGCAAAAATAGAGAGTCTGGACTCGTTGAAGAACTTGGAAGAAATTATTTTAGCATCAGATGGAGCTATGGTAGCTAGAGGAGATTTAGGAGCTCAAATACCACTGGAACAGGTGCCATCAGTCCAGCAAAAGGTCGTCCAACTGTGCAGACAATTAAATAAACCAGTTATTGTTGCTTCTCAGCTGCTTGAGTCAATGATCGAATATCCTACACCCACCAGAGCTGAAGTGGCTGATGTTTCTGAGGCGGTTAGGCAGCGATCAGATGCTCTAATGCTGTCTGGTGAGTCCGCCATGGGCCAGTACCCTGACAAGGCATTGGCTGTTTTGAGAAGTGTCAGTCTAAGAATTGAAAAGTGGTGGAGGGATGAGAAACGCCATGAACCTATGGAACTCCCAGAAGTTGGATCTTCATTCTCAGATAGTATCTTAGAAGAGATCTGCAATTCGGCTGCTAAAATGGCCAATAATTTGGAGGTAGATGCCATTTTCGTCTACACAACGTCAGGCCACATGGCATCTCTTCTGTCCCGTTGTCGACCTGATTGCCCAATCTTTGCTTTTACTTCCACAACATCTGTTAGGAGACGTCTTAACTTGCAATGGGGATTGATACCCTTCAGACTGAGCTTCTCCGACGACATGGAAAACAACCTCAACAAAACATTTTTGCTGCTTAAAGCCAGAAACTTGATCAAATCAGGTGACCTTGTAATTGCTGTCTCAGACATGTTGCAATCTATCCAAGTTATGAATGTTCCGTAA

Protein sequence

MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSSDLNPLSSSSTSQVLVSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Homology
BLAST of MELO3C014272 vs. NCBI nr
Match: XP_008449299.1 (PREDICTED: plastidial pyruvate kinase 1, chloroplastic [Cucumis melo] >KAA0057466.1 plastidial pyruvate kinase 1 [Cucumis melo var. makuwa] >TYK30163.1 plastidial pyruvate kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1129.0 bits (2919), Expect = 0.0e+00
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSSDLNPLSSSSTSQVL 60
           MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSSDLNPLSSSSTSQVL
Sbjct: 1   MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSSDLNPLSSSSTSQVL 60

Query: 61  VSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTIGP 120
           VSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTIGP
Sbjct: 61  VSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTIGP 120

Query: 121 ASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGSEI 180
           ASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGSEI
Sbjct: 121 ASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGSEI 180

Query: 181 HMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVR 240
           HMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVR
Sbjct: 181 HMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVR 240

Query: 241 FEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAE 300
           FEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAE
Sbjct: 241 FEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAE 300

Query: 301 GVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAMVA 360
           GVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAMVA
Sbjct: 301 GVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAMVA 360

Query: 361 RGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVR 420
           RGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVR
Sbjct: 361 RGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVR 420

Query: 421 QRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSILE 480
           QRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSILE
Sbjct: 421 QRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSILE 480

Query: 481 EICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLI 540
           EICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLI
Sbjct: 481 EICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLI 540

Query: 541 PFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           PFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 PFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 589

BLAST of MELO3C014272 vs. NCBI nr
Match: XP_004150564.1 (plastidial pyruvate kinase 1, chloroplastic [Cucumis sativus] >KGN61654.1 hypothetical protein Csa_006584 [Cucumis sativus])

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 583/591 (98.65%), Postives = 587/591 (99.32%), Query Frame = 0

Query: 1   MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRA-SSSDLNPLSSSSTSQV 60
           MTQSLQLFTSSAISLPKHSISKP SS+AAFRFPFSFSKSSIRA SSSDLNPLSSSSTSQV
Sbjct: 1   MTQSLQLFTSSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSSTSQV 60

Query: 61  LVSENGSSSGGGVV-SSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTI 120
           LVSENGSSSGGGVV SS+ATREFVSVASDSTSI+VDAVTEAELKENGFRSTRRTKLVCTI
Sbjct: 61  LVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKLVCTI 120

Query: 121 GPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGS 180
           GPA+CGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLND KGYAVAIMMDTEGS
Sbjct: 121 GPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDGKGYAVAIMMDTEGS 180

Query: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240
           EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM
Sbjct: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240

Query: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300
           VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI
Sbjct: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300

Query: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360
           AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM
Sbjct: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360

Query: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420
           VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA
Sbjct: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420

Query: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSI 480
           VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSI
Sbjct: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSI 480

Query: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540
           LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG
Sbjct: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540

Query: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 591

BLAST of MELO3C014272 vs. NCBI nr
Match: XP_038874381.1 (pyruvate kinase isozyme A, chloroplastic [Benincasa hispida])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 575/594 (96.80%), Postives = 584/594 (98.32%), Query Frame = 0

Query: 1   MTQSLQLFTSS----AISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSS-DLNPLSSSS 60
           MTQSLQLFTSS     ISLP+ S+SKP SS+AAFRFPFSFSKSSIRASSS DLNPLSSSS
Sbjct: 1   MTQSLQLFTSSTRSPGISLPRRSLSKPSSSSAAFRFPFSFSKSSIRASSSPDLNPLSSSS 60

Query: 61  TSQVLVSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGSSSG GVVSSSAT+EFVS+ASDSTSIDVDAVTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSSSGTGVVSSSATKEFVSLASDSTSIDVDAVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDT 180
           CTIGPASCGFEQLEALAVGGMNVARINMCHGTR+WHRTVIE VRRLNDEKGYAVAIMMDT
Sbjct: 121 CTIGPASCGFEQLEALAVGGMNVARINMCHGTREWHRTVIEYVRRLNDEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASD 360
           FGIAEGVDF+AISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII+ASD
Sbjct: 301 FGIAEGVDFIAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVASD 360

Query: 361 GAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADV 420
           GAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADV
Sbjct: 361 GAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADV 420

Query: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFS 480
           SEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFS
Sbjct: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFS 480

Query: 481 DSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNL 540
           D+ILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNL
Sbjct: 481 DTILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNL 540

Query: 541 QWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           QWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 QWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

BLAST of MELO3C014272 vs. NCBI nr
Match: XP_023548664.1 (pyruvate kinase isozyme A, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1057.4 bits (2733), Expect = 4.6e-305
Identity = 553/594 (93.10%), Postives = 569/594 (95.79%), Query Frame = 0

Query: 1   MTQSLQLFTSS----AISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSS-DLNPLSSSS 60
           MTQSLQLFTS+     ISL KHS+SKP  STAAFRFP SF+KSSIRASSS D NPLSS+ 
Sbjct: 1   MTQSLQLFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGS+ GGG VSSS TREFV V SDST+I+VD VTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSTVGGGAVSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDT 180
           CTIGP +CGFEQLEALAVGGMNVARINMCHGTR+WHR VIERVRRLN+EKGYAVAIMMDT
Sbjct: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRNWHRMVIERVRRLNEEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASD 360
           FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSL NLEEII+ASD
Sbjct: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLNNLEEIIVASD 360

Query: 361 GAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADV 420
           GAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD+
Sbjct: 361 GAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 420

Query: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFS 480
           SEAVRQRSDALMLSGESAMGQYPDKALAVLR+VSLRIEKWWR+EKRHEPMELPEVGSS S
Sbjct: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 480

Query: 481 DSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNL 540
           DSILEEICNSAAKMANNLEVDAIFVYT +GHMASLLSRCRPDCPIFAFT TTSVRRRLNL
Sbjct: 481 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 540

Query: 541 QWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           QWGLIPFR+SFSDDMENNLNK FLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

BLAST of MELO3C014272 vs. NCBI nr
Match: XP_023006710.1 (pyruvate kinase isozyme A, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1056.2 bits (2730), Expect = 1.0e-304
Identity = 551/594 (92.76%), Postives = 568/594 (95.62%), Query Frame = 0

Query: 1   MTQSLQLFTSS----AISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSS-DLNPLSSSS 60
           MTQSLQ+FTS+     ISL KHS+SKP  STAAFRFP SF+KSSIRASSS D NPLSS+ 
Sbjct: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGS+ GGG +SSS TREFV V SDST+I+VD VTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDT 180
           CTIGP +CGFEQLEALAVGGMNVARINMCHGTRDWHR VIERVRRLN+EKGYAVAIMMDT
Sbjct: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASD 360
           FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISI+AKIESLDSL NLEEIILASD
Sbjct: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASD 360

Query: 361 GAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADV 420
           GAMVARGDLGAQIPLEQVP VQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD+
Sbjct: 361 GAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 420

Query: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFS 480
           SEAVRQRSDALMLSGESAMGQYPDKALAVLR+VSLRIEKWWR+EKRHEPMELPEVGSS S
Sbjct: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 480

Query: 481 DSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNL 540
           DSILEEICNSAAKMANNLEVDAIFVYT +GHMASLLSRCRPDCPIFAFT TTSVRRRLNL
Sbjct: 481 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 540

Query: 541 QWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           QWGLIPFR+SFSDDMENNLNK FLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

BLAST of MELO3C014272 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 922.5 bits (2383), Expect = 2.3e-267
Identity = 480/599 (80.13%), Postives = 533/599 (88.98%), Query Frame = 0

Query: 1   MTQSLQLFTSS----AISLPKHSISKPFSSTAAFRFPFSFSK-SSIRASSS-----DLNP 60
           M+QS+Q  T S     + LP    ++P SS +  RFP +  K +SIRASSS     DL+ 
Sbjct: 1   MSQSIQFSTPSHTPHLLHLPHSQFNRPLSSISFRRFPLTTIKYTSIRASSSSSPSPDLDS 60

Query: 61  LSSSSTSQVLVSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTR 120
            SSSS+SQVL+S NG+   G V S   +    +V +D++ I+VD VTEAELKENGFRSTR
Sbjct: 61  SSSSSSSQVLLSPNGT---GAVKSDERSVVATAVTTDTSGIEVDTVTEAELKENGFRSTR 120

Query: 121 RTKLVCTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVA 180
           RTKL+CTIGPA+CGFEQLEALAVGGMNVAR+NMCHGTRDWHR VI  VRRLN+EKG+AVA
Sbjct: 121 RTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGFAVA 180

Query: 181 IMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD 240
           IMMDTEGSEIHMGDLGG +SAKAEDGE+WTF+VRAFDS+ PERTI+V+Y+GFAEDVRVGD
Sbjct: 181 IMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRVGD 240

Query: 241 DLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKD 300
           +LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKD
Sbjct: 241 ELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKD 300

Query: 301 WLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEI 360
           WLDIDFGIAEGVDF+A+SFVKSAEVI HLKSY+AARSRG +I +IAKIES+DSL NLEEI
Sbjct: 301 WLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLEEI 360

Query: 361 ILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRA 420
           ILASDGAMVARGDLGAQIPLEQVP+ QQ++VQ+CR LNKPVIVASQLLESMIEYPTPTRA
Sbjct: 361 ILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPTRA 420

Query: 421 EVADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEV 480
           EVADVSEAVRQRSDALMLSGESAMGQ+PDKAL VLR+VSLRIE+WWR+EKRHE + L  +
Sbjct: 421 EVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQAI 480

Query: 481 GSSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVR 540
           GSSFSD I EEICNSAAKMANNL VDA+FVYTTSGHMASL+SRCRPDCPIFAFT+TTSVR
Sbjct: 481 GSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTTSVR 540

Query: 541 RRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           RRLNLQWGLIPFRLSFSDDME+NLNKTF LLK+R +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 RRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSIQVMNVP 596

BLAST of MELO3C014272 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 1.7e-265
Identity = 484/593 (81.62%), Postives = 534/593 (90.05%), Query Frame = 0

Query: 1   MTQSLQL---FTSSAISLPKHSISKPFSSTAAFRFPFSFSKS-SIRASSSDLNPLSSSST 60
           M+QSL      T +    PK  +  P S++   R+P +  KS SI+AS+S     SSSS 
Sbjct: 1   MSQSLHFSPNLTFAKQPFPKLPLPFPTSNS---RYPVNNYKSLSIKASTSP----SSSSD 60

Query: 61  SQVLVSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVC 120
            QVLV++NG+ + G + +++   + V+V SD +SI+VDAVTE ELKENGFRSTRRTKLVC
Sbjct: 61  PQVLVADNGTGNSGVLYNNN--NKSVTV-SDPSSIEVDAVTETELKENGFRSTRRTKLVC 120

Query: 121 TIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTE 180
           TIGPA+CGFE+LEALAVGGMNVARINMCHGTR+WH++VIERVRRLN+EKG+AVAIMMDTE
Sbjct: 121 TIGPATCGFEELEALAVGGMNVARINMCHGTREWHKSVIERVRRLNEEKGFAVAIMMDTE 180

Query: 181 GSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDG 240
           GSEIHMGDLGGASSAKAEDGEIWTFSVRA+DS  PERTINVNY+GFAEDV+VGD+LLVDG
Sbjct: 181 GSEIHMGDLGGASSAKAEDGEIWTFSVRAYDSPRPERTINVNYDGFAEDVKVGDELLVDG 240

Query: 241 GMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDF 300
           GMVRFEVIEKIGPDVKC CTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKDWLDIDF
Sbjct: 241 GMVRFEVIEKIGPDVKCRCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKDWLDIDF 300

Query: 301 GIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDG 360
           GIAEGVDF+AISFVKSAEVI HLKSYIAARSR SDI++IAKIES+DSLKNLEEII ASDG
Sbjct: 301 GIAEGVDFIAISFVKSAEVINHLKSYIAARSRDSDIAVIAKIESIDSLKNLEEIIRASDG 360

Query: 361 AMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVS 420
           AMVARGDLGAQIPLEQVPS QQ +VQ+CRQLNKPVIVASQLLESMIEYPTPTRAEVADVS
Sbjct: 361 AMVARGDLGAQIPLEQVPSAQQNIVQVCRQLNKPVIVASQLLESMIEYPTPTRAEVADVS 420

Query: 421 EAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSD 480
           EAVRQR+DALMLSGESAMGQYP+KALAVLRSVS+RIEKWWR+EK HE MELP +GS++SD
Sbjct: 421 EAVRQRADALMLSGESAMGQYPEKALAVLRSVSVRIEKWWREEKHHEAMELPAIGSTYSD 480

Query: 481 SILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQ 540
           SI EEICNSAAKMANNL VDA+FVYT  GHMASLLSRCRPDCPIFAFT+TTSVRRRLNLQ
Sbjct: 481 SISEEICNSAAKMANNLGVDALFVYTKDGHMASLLSRCRPDCPIFAFTTTTSVRRRLNLQ 540

Query: 541 WGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           WGLIPFRLSF+DDME+NLNKTF LLKAR +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 WGLIPFRLSFADDMESNLNKTFSLLKARGMIKSGDLVIAVSDMLQSIQVMNVP 583

BLAST of MELO3C014272 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 2.4e-264
Identity = 479/597 (80.23%), Postives = 531/597 (88.94%), Query Frame = 0

Query: 1   MTQSLQLFTSSAISLP-KHSISKP--FSSTAAFRFPFSFSKSS--IRASSSDLNPLSSSS 60
           M+Q+L  F SS+   P   +IS+P  F ST + R     S  +  + ASS+  + L    
Sbjct: 1   MSQALNFFVSSSSRSPATFTISRPSVFPSTGSLRLLVKKSLRTLVVEASSAAASDLDEPQ 60

Query: 61  TSQVLVSENGSSSGGGVVSSSATREF---VSVASDSTSIDVDAVTEAELKENGFRSTRRT 120
           +S VLVSENGS   GGV+ SSAT+E+    +  +DS+SI+VD VTEAELKENGFRSTRRT
Sbjct: 61  SSPVLVSENGS---GGVL-SSATQEYGRNAAPGTDSSSIEVDTVTEAELKENGFRSTRRT 120

Query: 121 KLVCTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIM 180
           KL+CTIGPA+CGFEQLE LA GGMNVARINMCHGTR+WHR VIER+RRLN+EKG+AVAIM
Sbjct: 121 KLICTIGPATCGFEQLERLAEGGMNVARINMCHGTREWHRMVIERLRRLNEEKGFAVAIM 180

Query: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240
           MDTEGSEIHMGDLGGASSAKAEDGEIW F+VR+FD  LPERT+ VNY+GFAEDV+VGD+L
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDPPLPERTVTVNYDGFAEDVKVGDEL 240

Query: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGKLVRERNAMLPTISSKDWL 300

Query: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIL 360
           DIDFGIAEGVDF+A+SFVKSAEVIKHLKSYI AR+R SDIS+IAKIES+DSLKNLEEII 
Sbjct: 301 DIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARARDSDISVIAKIESIDSLKNLEEIIQ 360

Query: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420
           ASDGAMVARGDLGAQIPLEQVPS QQK+VQ+CRQLN+PVIVASQLLESMIEYP PTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLNRPVIVASQLLESMIEYPIPTRAEV 420

Query: 421 ADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGS 480
           ADVSEAVRQR DALMLSGESAMGQ+P+KAL VLRSVSLRIE+ WR++KRHE +ELP + S
Sbjct: 421 ADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSVSLRIERMWREQKRHEVIELPSIAS 480

Query: 481 SFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRR 540
           SFSDSI EEICNSAAKMANNLEVDA+FVYT +GHMASLLSRCRPDCPIFAFT+TTSVRRR
Sbjct: 481 SFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMASLLSRCRPDCPIFAFTTTTSVRRR 540

Query: 541 LNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           LNLQWGL+PFRLSFSDDME+NLNKTF LLKAR +IKSGDL+IAVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIKSGDLIIAVSDMLQSIQVMNVP 593

BLAST of MELO3C014272 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 4.9e-100
Identity = 209/477 (43.82%), Postives = 297/477 (62.26%), Query Frame = 0

Query: 108 STRRTKLVCTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDE-KG 167
           S R+TK+VCTIGP++   E +  LA  GMNVAR+NM HG    H+  I+ V+  N + + 
Sbjct: 89  SPRKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFED 148

Query: 168 YAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDV 227
             +AIM+DT+G E+  GD+        ++G+ + FS++   ST  E T++VNY+ F  DV
Sbjct: 149 KVIAIMLDTKGPEVISGDV--PKPILLKEGQEFNFSIKRGVST--EDTVSVNYDDFINDV 208

Query: 228 RVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTI 287
             GD LLVDGGM+   V  K    VKC   D G L  R +L        VR ++A LP+I
Sbjct: 209 EAGDILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSRRHLN-------VRGKSATLPSI 268

Query: 288 SSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKN 347
           + KDW DI FG+   VDF A+SFVK A+V+  LK Y+  +S  +DI +I KIES DS+ N
Sbjct: 269 TEKDWDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYL--KSCNADIHVIVKIESADSIPN 328

Query: 348 LEEIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPT 407
           L  II ASDGAMVARGDLGA++P+E+VP +Q+ +++ C+ + KPVIVA+ +LESMI++PT
Sbjct: 329 LHSIISASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPT 388

Query: 408 PTRAEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPME 467
           PTRAEV+D+S AVR+ +DA+MLSGE+A G+YP KA+ V+  V+LR E         +   
Sbjct: 389 PTRAEVSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALRTE-----SSLQKSTS 448

Query: 468 LPEVGSSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTST 527
            P   +++   + E     ++ MAN L    I V+T +G MA +LS  RP   +FAFT+ 
Sbjct: 449 SPSQSAAYKSHMGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFTNN 508

Query: 528 TSVRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSI 584
             V++RL L  G++P  + FS D E   ++   LL +++L+K G  V  V    Q I
Sbjct: 509 ERVKQRLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLVQSGAQPI 546

BLAST of MELO3C014272 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 9.3e-99
Identity = 206/475 (43.37%), Postives = 292/475 (61.47%), Query Frame = 0

Query: 110 RRTKLVCTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDE-KGYA 169
           R+TK+VCT+GP++   E +  LA  GMNVAR+NM HG    H+ VI+ V+  N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 170 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRV 229
           +AIM+DT+G E+  GDL        + G+ +TF++    ST     ++VNY+ F  DV  
Sbjct: 170 IAIMLDTKGPEVRSGDL--PQPIMLDPGQEFTFTIERGVST--PSCVSVNYDDFVNDVEA 229

Query: 230 GDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISS 289
           GD LLVDGGM+ F V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 230 GDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSITE 289

Query: 290 KDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLE 349
           KDW DI FG+   VDF A+SFVK A+V+  LK Y+  ++ G+DI +I KIES DS+ NL 
Sbjct: 290 KDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYL--QNSGADIHVIVKIESADSIPNLH 349

Query: 350 EIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPT 409
            II ASDGAMVARGDLGA++P+E+VP +Q++++ LCR + K VIVA+ +LESMI +PTPT
Sbjct: 350 SIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPT 409

Query: 410 RAEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELP 469
           RAEV+D++ AVR+ +DA+MLSGE+A G++P KA  V+ +V+LR E      +       P
Sbjct: 410 RAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMP-----P 469

Query: 470 EVGSSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTS 529
            +G +F + + E     A  M+N L    + V+T +G MA LLS  RP   I+AFT+   
Sbjct: 470 NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKK 529

Query: 530 VRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSI 584
           +++RL L  G+ P  + F+DD E         L  + ++K G+ +  V    Q I
Sbjct: 530 IQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPI 565

BLAST of MELO3C014272 vs. ExPASy TrEMBL
Match: A0A5A7UTK2 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001510 PE=3 SV=1)

HSP 1 Score: 1129.0 bits (2919), Expect = 0.0e+00
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSSDLNPLSSSSTSQVL 60
           MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSSDLNPLSSSSTSQVL
Sbjct: 1   MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSSDLNPLSSSSTSQVL 60

Query: 61  VSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTIGP 120
           VSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTIGP
Sbjct: 61  VSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTIGP 120

Query: 121 ASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGSEI 180
           ASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGSEI
Sbjct: 121 ASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGSEI 180

Query: 181 HMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVR 240
           HMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVR
Sbjct: 181 HMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVR 240

Query: 241 FEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAE 300
           FEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAE
Sbjct: 241 FEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAE 300

Query: 301 GVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAMVA 360
           GVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAMVA
Sbjct: 301 GVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAMVA 360

Query: 361 RGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVR 420
           RGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVR
Sbjct: 361 RGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVR 420

Query: 421 QRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSILE 480
           QRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSILE
Sbjct: 421 QRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSILE 480

Query: 481 EICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLI 540
           EICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLI
Sbjct: 481 EICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLI 540

Query: 541 PFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           PFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 PFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 589

BLAST of MELO3C014272 vs. ExPASy TrEMBL
Match: A0A1S3BLQ9 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103491219 PE=3 SV=1)

HSP 1 Score: 1129.0 bits (2919), Expect = 0.0e+00
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSSDLNPLSSSSTSQVL 60
           MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSSDLNPLSSSSTSQVL
Sbjct: 1   MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSSDLNPLSSSSTSQVL 60

Query: 61  VSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTIGP 120
           VSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTIGP
Sbjct: 61  VSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTIGP 120

Query: 121 ASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGSEI 180
           ASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGSEI
Sbjct: 121 ASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGSEI 180

Query: 181 HMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVR 240
           HMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVR
Sbjct: 181 HMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVR 240

Query: 241 FEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAE 300
           FEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAE
Sbjct: 241 FEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAE 300

Query: 301 GVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAMVA 360
           GVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAMVA
Sbjct: 301 GVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAMVA 360

Query: 361 RGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVR 420
           RGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVR
Sbjct: 361 RGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVR 420

Query: 421 QRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSILE 480
           QRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSILE
Sbjct: 421 QRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSILE 480

Query: 481 EICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLI 540
           EICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLI
Sbjct: 481 EICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLI 540

Query: 541 PFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           PFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 PFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 589

BLAST of MELO3C014272 vs. ExPASy TrEMBL
Match: A0A0A0LKS3 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G215480 PE=3 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 583/591 (98.65%), Postives = 587/591 (99.32%), Query Frame = 0

Query: 1   MTQSLQLFTSSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRA-SSSDLNPLSSSSTSQV 60
           MTQSLQLFTSSAISLPKHSISKP SS+AAFRFPFSFSKSSIRA SSSDLNPLSSSSTSQV
Sbjct: 1   MTQSLQLFTSSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSSTSQV 60

Query: 61  LVSENGSSSGGGVV-SSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTI 120
           LVSENGSSSGGGVV SS+ATREFVSVASDSTSI+VDAVTEAELKENGFRSTRRTKLVCTI
Sbjct: 61  LVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKLVCTI 120

Query: 121 GPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDTEGS 180
           GPA+CGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLND KGYAVAIMMDTEGS
Sbjct: 121 GPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDGKGYAVAIMMDTEGS 180

Query: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240
           EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM
Sbjct: 181 EIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGM 240

Query: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300
           VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI
Sbjct: 241 VRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGI 300

Query: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360
           AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM
Sbjct: 301 AEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASDGAM 360

Query: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420
           VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA
Sbjct: 361 VARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEA 420

Query: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSI 480
           VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSI
Sbjct: 421 VRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFSDSI 480

Query: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540
           LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG
Sbjct: 481 LEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWG 540

Query: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 591

BLAST of MELO3C014272 vs. ExPASy TrEMBL
Match: A0A6J1L0W9 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1)

HSP 1 Score: 1056.2 bits (2730), Expect = 5.0e-305
Identity = 551/594 (92.76%), Postives = 568/594 (95.62%), Query Frame = 0

Query: 1   MTQSLQLFTSS----AISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSS-DLNPLSSSS 60
           MTQSLQ+FTS+     ISL KHS+SKP  STAAFRFP SF+KSSIRASSS D NPLSS+ 
Sbjct: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGS+ GGG +SSS TREFV V SDST+I+VD VTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDT 180
           CTIGP +CGFEQLEALAVGGMNVARINMCHGTRDWHR VIERVRRLN+EKGYAVAIMMDT
Sbjct: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASD 360
           FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISI+AKIESLDSL NLEEIILASD
Sbjct: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASD 360

Query: 361 GAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADV 420
           GAMVARGDLGAQIPLEQVP VQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD+
Sbjct: 361 GAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 420

Query: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFS 480
           SEAVRQRSDALMLSGESAMGQYPDKALAVLR+VSLRIEKWWR+EKRHEPMELPEVGSS S
Sbjct: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 480

Query: 481 DSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNL 540
           DSILEEICNSAAKMANNLEVDAIFVYT +GHMASLLSRCRPDCPIFAFT TTSVRRRLNL
Sbjct: 481 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 540

Query: 541 QWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           QWGLIPFR+SFSDDMENNLNK FLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

BLAST of MELO3C014272 vs. ExPASy TrEMBL
Match: A0A6J1KDQ9 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111494735 PE=3 SV=1)

HSP 1 Score: 1052.4 bits (2720), Expect = 7.2e-304
Identity = 554/597 (92.80%), Postives = 572/597 (95.81%), Query Frame = 0

Query: 1   MTQSLQLFTSS-----AISLPKHSISK-PFSSTAAFRFPFSFSKSSIRASSS-DLNPLSS 60
           M QSLQLFTSS     +ISLPKHS+SK P SSTAAFR P SF+K SIRASSS DLNPLSS
Sbjct: 1   MAQSLQLFTSSTTRSPSISLPKHSLSKTPSSSTAAFRLPVSFNKFSIRASSSPDLNPLSS 60

Query: 61  SSTSQVLVSENGSSSGGGVVSSSA-TREFVSVASDSTSIDVDAVTEAELKENGFRSTRRT 120
           +STSQVLVSENGSSSGGG VS+SA TREFV   SD +SIDVD VTEAELKENGFRSTRRT
Sbjct: 61  ASTSQVLVSENGSSSGGGFVSTSATTREFVPAVSDGSSIDVDTVTEAELKENGFRSTRRT 120

Query: 121 KLVCTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIM 180
           KLVCTIGP +CGFEQLEALAVGGMNVARINMCHGTR+WHR VIERVRRLN+EKGYAVAIM
Sbjct: 121 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTREWHRMVIERVRRLNEEKGYAVAIM 180

Query: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240

Query: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIL 360
           DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEII+
Sbjct: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIV 360

Query: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420
           ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR+LNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRRLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 421 ADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGS 480
           ADVSEAVRQR+DALMLSGESAMGQ+P+KAL VLRSVSLRIEKWWRDEKRHE MELPEVGS
Sbjct: 421 ADVSEAVRQRADALMLSGESAMGQFPEKALTVLRSVSLRIEKWWRDEKRHEAMELPEVGS 480

Query: 481 SFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRR 540
           S+SDSILEEICNS AKMANNLEVDAIFVYT +GHMASLLSRCRPDCPIFAFTSTTSVRRR
Sbjct: 481 SYSDSILEEICNSTAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTSTTSVRRR 540

Query: 541 LNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           LNLQWGLIPFRLSFSDDMENNLNKTF+LLKARNLIKSGDLV+AVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRLSFSDDMENNLNKTFMLLKARNLIKSGDLVLAVSDMLQSIQVMNVP 597

BLAST of MELO3C014272 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 922.5 bits (2383), Expect = 1.7e-268
Identity = 480/599 (80.13%), Postives = 533/599 (88.98%), Query Frame = 0

Query: 1   MTQSLQLFTSS----AISLPKHSISKPFSSTAAFRFPFSFSK-SSIRASSS-----DLNP 60
           M+QS+Q  T S     + LP    ++P SS +  RFP +  K +SIRASSS     DL+ 
Sbjct: 1   MSQSIQFSTPSHTPHLLHLPHSQFNRPLSSISFRRFPLTTIKYTSIRASSSSSPSPDLDS 60

Query: 61  LSSSSTSQVLVSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTR 120
            SSSS+SQVL+S NG+   G V S   +    +V +D++ I+VD VTEAELKENGFRSTR
Sbjct: 61  SSSSSSSQVLLSPNGT---GAVKSDERSVVATAVTTDTSGIEVDTVTEAELKENGFRSTR 120

Query: 121 RTKLVCTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVA 180
           RTKL+CTIGPA+CGFEQLEALAVGGMNVAR+NMCHGTRDWHR VI  VRRLN+EKG+AVA
Sbjct: 121 RTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGFAVA 180

Query: 181 IMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD 240
           IMMDTEGSEIHMGDLGG +SAKAEDGE+WTF+VRAFDS+ PERTI+V+Y+GFAEDVRVGD
Sbjct: 181 IMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRVGD 240

Query: 241 DLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKD 300
           +LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKD
Sbjct: 241 ELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKD 300

Query: 301 WLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEI 360
           WLDIDFGIAEGVDF+A+SFVKSAEVI HLKSY+AARSRG +I +IAKIES+DSL NLEEI
Sbjct: 301 WLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLEEI 360

Query: 361 ILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRA 420
           ILASDGAMVARGDLGAQIPLEQVP+ QQ++VQ+CR LNKPVIVASQLLESMIEYPTPTRA
Sbjct: 361 ILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPTRA 420

Query: 421 EVADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEV 480
           EVADVSEAVRQRSDALMLSGESAMGQ+PDKAL VLR+VSLRIE+WWR+EKRHE + L  +
Sbjct: 421 EVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQAI 480

Query: 481 GSSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVR 540
           GSSFSD I EEICNSAAKMANNL VDA+FVYTTSGHMASL+SRCRPDCPIFAFT+TTSVR
Sbjct: 481 GSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTTSVR 540

Query: 541 RRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 590
           RRLNLQWGLIPFRLSFSDDME+NLNKTF LLK+R +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 RRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSIQVMNVP 596

BLAST of MELO3C014272 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 362.5 bits (929), Expect = 6.6e-100
Identity = 206/475 (43.37%), Postives = 292/475 (61.47%), Query Frame = 0

Query: 110 RRTKLVCTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDE-KGYA 169
           R+TK+VCT+GP++   E +  LA  GMNVAR+NM HG    H+ VI+ V+  N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 170 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRV 229
           +AIM+DT+G E+  GDL        + G+ +TF++    ST     ++VNY+ F  DV  
Sbjct: 170 IAIMLDTKGPEVRSGDL--PQPIMLDPGQEFTFTIERGVST--PSCVSVNYDDFVNDVEA 229

Query: 230 GDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISS 289
           GD LLVDGGM+ F V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 230 GDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSITE 289

Query: 290 KDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLE 349
           KDW DI FG+   VDF A+SFVK A+V+  LK Y+  ++ G+DI +I KIES DS+ NL 
Sbjct: 290 KDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYL--QNSGADIHVIVKIESADSIPNLH 349

Query: 350 EIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPT 409
            II ASDGAMVARGDLGA++P+E+VP +Q++++ LCR + K VIVA+ +LESMI +PTPT
Sbjct: 350 SIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPT 409

Query: 410 RAEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELP 469
           RAEV+D++ AVR+ +DA+MLSGE+A G++P KA  V+ +V+LR E      +       P
Sbjct: 410 RAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMP-----P 469

Query: 470 EVGSSFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTS 529
            +G +F + + E     A  M+N L    + V+T +G MA LLS  RP   I+AFT+   
Sbjct: 470 NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKK 529

Query: 530 VRRRLNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSI 584
           +++RL L  G+ P  + F+DD E         L  + ++K G+ +  V    Q I
Sbjct: 530 IQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPI 565

BLAST of MELO3C014272 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 361.3 bits (926), Expect = 1.5e-99
Identity = 216/505 (42.77%), Postives = 310/505 (61.39%), Query Frame = 0

Query: 85  ASDSTSIDVDAVTEAELKENGFRSTRRTKLVCTIGPASCGFEQLEALAVGGMNVARINMC 144
           A D   +D  +   A+ + +   S R+TK+VCTIGP+S   E +  LA  GMNVAR+NM 
Sbjct: 74  AFDVGVLDSSSYRLADSRTSSNDSRRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMS 133

Query: 145 HGTRDWHRTVIERVRRLND-EKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSV 204
           HG    H+  I+ V+  N      A+AIM+DT+G E+  GD+        E+G+ + F++
Sbjct: 134 HGDHASHQITIDLVKEYNSLFVDKAIAIMLDTKGPEVRSGDV--PQPIFLEEGQEFNFTI 193

Query: 205 RAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLP 264
           +   S   + T++VNY+ F  DV VGD LLVDGGM+   V  K    VKC+  D G L  
Sbjct: 194 KRGVSL--KDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQS 253

Query: 265 RANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYI 324
           R +L        VR ++A LP+I+ KDW DI FG+   VDF A+SFVK A+V+  LK+Y+
Sbjct: 254 RRHLN-------VRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYL 313

Query: 325 AARSRGSDISIIAKIESLDSLKNLEEIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQL 384
             ++  +DIS+I KIES DS+KNL  II A DGAMVARGDLGA++P+E+VP +Q+++++ 
Sbjct: 314 --KTCSADISVIVKIESADSIKNLPSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRR 373

Query: 385 CRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQYPDKALA 444
           CR ++KPVIVA+ +LESMI +PTPTRAEV+D++ AVR+ +DA+MLSGE+A G++P KA+ 
Sbjct: 374 CRSIHKPVIVATNMLESMINHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVN 433

Query: 445 VLRSVSLRIEKWWRDEKRHEPMELPEVGS-----SFSDSILEEICNSAAKMANNLEVDAI 504
           V+ +V+LR E             LP   S     ++   + +     A+ MAN L    +
Sbjct: 434 VMHTVALRTE-----------ASLPVRTSASRTTAYKGHMGQMFAFHASIMANTLS-SPL 493

Query: 505 FVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSDDMENNLNKTF 564
            V+T +G MA LLS  RP   IFAFT+   + +RL L  G++P  + FSDD E+   ++ 
Sbjct: 494 IVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQRLALYQGVMPIYMEFSDDAEDTYARSL 553

Query: 565 LLLKARNLIKSGDLVIAVSDMLQSI 584
            LL+  N++K G  V  V    Q I
Sbjct: 554 KLLQDENMLKEGQHVTLVQSGSQPI 553

BLAST of MELO3C014272 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 242.7 bits (618), Expect = 7.6e-64
Identity = 162/516 (31.40%), Postives = 270/516 (52.33%), Query Frame = 0

Query: 89  TSIDVDAVTEAELKENGFRSTRRTKLVCTIGPASCGFEQLEALAVGGMNVARINMCHGTR 148
           ++ID++ + + EL  +G   T +TK+VCT+GPAS     +E L   GMNVAR N  HG+ 
Sbjct: 2   SNIDIEGILK-ELPNDG--RTPKTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSH 61

Query: 149 DWHRTVIERVRRLNDEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDS 208
           ++H+  ++ +R      G   A+M+DT+G EI  G L   +  + ++G+  T +   +D 
Sbjct: 62  EYHQETLDNLRTAMQNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITT-DYDI 121

Query: 209 TLPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVI--EKIGPDVKCLCTDPGLLLPRAN 268
              E+TI+++Y+    DV+ G+ +L   G +   V+  +     V C C +  +L  R N
Sbjct: 122 KGDEKTISMSYKKLPVDVKPGNTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKN 181

Query: 269 LTFWRDGHLVRERNAMLPTISSKDWLDI-DFGIAEGVDFLAISFVKSAEVIKHLKSYIAA 328
           +     G +V      LPT++ KD  DI  +G+   +D +A+SFV+    + +++  + +
Sbjct: 182 VNL--PGVVVD-----LPTLTDKDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGS 241

Query: 329 RSRGSDISIIAKIESLDSLKNLEEIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR 388
            S+   I +++K+E+ + + N +EI+  +D  MVARGDLG +IP+E++   Q+ ++  C 
Sbjct: 242 HSK--SIMLMSKVENQEGVLNFDEILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCN 301

Query: 389 QLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVL 448
              KPV+ A+Q+LESMI+ P PTRAE  DV+ AV   +D +MLSGESA G YP+ A+  +
Sbjct: 302 LAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKTM 361

Query: 449 RSVSLRIEK------WWRDEKRHEPMELPEVGSSFSDSILEEICNSAAKMANNLEVDAIF 508
             + +  E        +++  R  P+ +         S LE + +SA + AN  +   I 
Sbjct: 362 AKICIEAESSLDYNTIFKEMIRATPLPM---------STLESLASSAVRTANKAKAKLII 421

Query: 509 VYTTSGHMASLLSRCRPDCPIFA-----FTSTT--------SVRRRLNLQWGLIPF---- 568
           V T  G  A L+++ RP  PI +     FTS T        S  R   +  GLIP     
Sbjct: 422 VLTRGGTTAKLVAKYRPAVPILSVVVPVFTSDTFNWSCSDESPARHSLIYRGLIPVLGEG 481

Query: 569 --RLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAV 577
             + + S+  E  +         + L   GD V+A+
Sbjct: 482 SAKATDSESTEEIIESALKSATEKGLCNHGDAVVAL 495

BLAST of MELO3C014272 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 235.0 bits (598), Expect = 1.6e-61
Identity = 159/516 (30.81%), Postives = 265/516 (51.36%), Query Frame = 0

Query: 89  TSIDVDAVTEAELKENGFRSTRRTKLVCTIGPASCGFEQLEALAVGGMNVARINMCHGTR 148
           ++ID++ + + EL  +G     +TK+VCT+GPAS     +E L   GMNVAR N  HG+ 
Sbjct: 2   SNIDIEGILK-ELPNDG--RIPKTKIVCTLGPASRTVSMIEKLLKAGMNVARFNFSHGSH 61

Query: 149 DWHRTVIERVRRLNDEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDS 208
           ++H+  ++ +R      G   A+M+DT+G EI  G L   +  + ++G+  T +   +D 
Sbjct: 62  EYHQETLDNLRSAMHNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITT-DYDI 121

Query: 209 TLPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVI--EKIGPDVKCLCTDPGLLLPRAN 268
              E TI+++Y+    DV+ G+ +L   G +   V+  +     V+C C +  +L  R N
Sbjct: 122 QGDESTISMSYKKLPLDVKPGNTILCADGSISLAVLSCDPESGTVRCRCENSAMLGERKN 181

Query: 269 LTFWRDGHLVRERNAMLPTISSKDWLDI-DFGIAEGVDFLAISFVKSAEVIKHLKSYIAA 328
           +     G +V      LPT++ KD  DI  +G+   +D +A+SFV+    + +++  +  
Sbjct: 182 VNL--PGVVVD-----LPTLTDKDIEDILGWGVPNSIDMIALSFVRKGSDLVNVRKVLG- 241

Query: 329 RSRGSDISIIAKIESLDSLKNLEEIILASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCR 388
            S    I +++K+E+ + + N +EI+  +D  MVARGDLG +IP+E++   Q+ ++  C 
Sbjct: 242 -SHAKSIMLMSKVENQEGVINFDEILRETDAFMVARGDLGMEIPIEKIFLAQKLMIYKCN 301

Query: 389 QLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQYPDKALAVL 448
              KPV+ A+Q+LESMI+ P PTRAE  DV+ AV   +D +MLSGESA G YP+ A+ V+
Sbjct: 302 LAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKVM 361

Query: 449 RSVSLRIEK------WWRDEKRHEPMELPEVGSSFSDSILEEICNSAAKMANNLEVDAIF 508
             + +  E        +++  R  P+ +         S LE + +SA + AN      I 
Sbjct: 362 AKICIEAESSLDYNTIFKEMIRATPLPM---------SPLESLASSAVRTANKARAKLII 421

Query: 509 VYTTSGHMASLLSRCRPDCPIFAFT-------------STTSVRRRLNLQWGLIPF---- 568
           V T  G  A+L+++ RP  PI +               S  S  R   +  GLIP     
Sbjct: 422 VLTRGGSTANLVAKYRPAVPILSVVVPVMTTDSFDWSCSDESPARHSLIYRGLIPMLAEG 481

Query: 569 --RLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAV 577
             + + S+  E  +         R L   GD ++A+
Sbjct: 482 SAKATDSEATEVIIEAALKSATQRGLCNRGDAIVAL 495

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008449299.10.0e+00100.00PREDICTED: plastidial pyruvate kinase 1, chloroplastic [Cucumis melo] >KAA005746... [more]
XP_004150564.10.0e+0098.65plastidial pyruvate kinase 1, chloroplastic [Cucumis sativus] >KGN61654.1 hypoth... [more]
XP_038874381.10.0e+0096.80pyruvate kinase isozyme A, chloroplastic [Benincasa hispida][more]
XP_023548664.14.6e-30593.10pyruvate kinase isozyme A, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_023006710.11.0e-30492.76pyruvate kinase isozyme A, chloroplastic-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LIK02.3e-26780.13Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q431171.7e-26581.62Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Q405452.4e-26480.23Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q405464.9e-10043.82Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9FLW99.3e-9943.37Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7UTK20.0e+00100.00Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G0015... [more]
A0A1S3BLQ90.0e+00100.00Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103491219 PE=3 SV=1[more]
A0A0A0LKS30.0e+0098.65Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G215480 PE=3 SV=1[more]
A0A6J1L0W95.0e-30592.76Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1[more]
A0A6J1KDQ97.2e-30492.80Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111494735 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G22960.11.7e-26880.13Pyruvate kinase family protein [more]
AT5G52920.16.6e-10043.37plastidic pyruvate kinase beta subunit 1 [more]
AT1G32440.11.5e-9942.77plastidial pyruvate kinase 3 [more]
AT5G63680.17.6e-6431.40Pyruvate kinase family protein [more]
AT5G08570.11.6e-6130.81Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 430..446
score: 41.81
coord: 361..385
score: 45.91
coord: 386..410
score: 60.31
coord: 334..360
score: 52.14
coord: 167..183
score: 45.07
coord: 411..429
score: 51.34
coord: 302..316
score: 41.74
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 110..578
e-value: 1.3E-127
score: 424.1
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 71..589
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 453..579
e-value: 5.5E-151
score: 505.2
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 441..587
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 110..455
e-value: 1.6E-91
score: 306.8
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 480..579
e-value: 3.2E-23
score: 82.2
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 178..284
e-value: 5.5E-151
score: 505.2
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 110..452
e-value: 5.5E-151
score: 505.2
NoneNo IPR availablePANTHERPTHR11817:SF14PLASTIDIAL PYRUVATE KINASE 1, CHLOROPLASTICcoord: 71..589
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 332..344
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 179..270
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 96..456

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C014272.1MELO3C014272.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
molecular_function GO:0003824 catalytic activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity