MELO3C012767 (gene) Melon (DHL92) v4

Overview
NameMELO3C012767
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
Description3-methyl-2-oxobutanoate hydroxymethyltransferase
Locationchr07: 14236410 .. 14242378 (+)
RNA-Seq ExpressionMELO3C012767
SyntenyMELO3C012767
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATATTCCGTACTTAAGCCACTCAGTACTTGATGCTCTACGAAGCATGCTCTCAGGCTTCAACCATTTGTTCGTGATTTAGTTGGAGTAACGTTGGTGAGCAATCGATCAATGGCGCTATTCAAGCTTTTAACTATGGCCGCATCTCGAACAAGAAAACAATCCCTCCTCAAAACCCTTCGTTACATGAGCAATGTCCCTGAAAATACGGTCTACAGCGGCCCCACCTCCCAAACTCCTAATCAAAGAGTCACTCTCACCAATCTTAGACAAAAGTACAAGAAAGGTGAACCCATCACCATGGTCACTGCTTACGATTACCCTTCCGGTGTCCATTTGGACATGGCTGGGATTGATATTGCTTTGGTTGGCGATTCGGCTGCCATGGTTGTGCACGGCCACGACACTACGTTACCGATTAGTTTGGATGAAATCCTTGTTCATTGTAGGGCTGTTGCTCGTGGGGCTAGGAGACCTCTTTTGATTGGTGATTTGCCGTTTGGTACTTATGAATCCAGCTCTGCGCAGGTTTTTTTTTCCTCTTTTCCTTTAGGTGTTTGAAGAAATGACAAAGTTAGAGAATGTTTGTTTTTCTGTTGTCGTTTGAATGGGCTGTACGCGGAATTTAAGGGATTGTGAAAATGTCTACTTTTACTTGCTGATGGAGTTATTTAAAAGATTGAAAATGGTAAGTCTTGCATTCATATCAAAATATGTTTTCATTTACTTTCTGGTTAGATTAGGGCATCATAATGTCTCAAAATGCATAAACAGACCCAAATTACTTTTTGTTGGTATTTTGTTGCTTTTCTTTTTACAACCATCACTTGCTGTAAAGTAAATGCTTCACTTTTCCATTTTTATACTTCCTGTTCTCTTGTGACTCCTTTCAAGTGGGGTTTCTACCCTTTTCTTTGTAATTTCATAACACCAATAAAACTGATTCCCATACTAATTATTAAAGGAAAAAAGACATAAACTTGAAACGAACATATCTTGTGGAGTAATTTATTTTGATAATTCGCTAGTTTAGAAATGTGTATATTTTGATGGGAAACTAGAGTGGAATTGGTAGCTGTAATTGAATCGTACTAGCAACCTTTTTTAGGATGTCAAAACCTTAAACAAATCAGTAACTTCTATTGGAGAGGTCGAGCCTGAGGCTAAAGGCATTGTGGCATGTGGGTGTAAACTGCATTCATGGAAGGGCTAGAGAAAATCTCAGTAGAGGATCGACTGATATTTGAGTTTTAGGCATATTATGGAATGGAAACACAGTTTCATGGAAGACCACGTCTCTTGGGATGAAGAACTTTTTGTGCTCAATGTCATACAGCTTGTAAAGCCTTCATGCCATGGGGATAGCCGATGAAGACAGAGGGAACAACTCTGGGATGAACTTGGAGCGATTGTGAGACAGTGAGGATGCATAGCATAAAGAGCCAAAAATTCTTAAAGAGATATAGTCAGGTACCGTGTTATTCAACTTCTGAAATGGAAACTCCCATTGCAATAACATGGATGGAGTTCTATTAATCAAATAAATGGCTGTTAGTATACATTCTCCCCAAAATGTTAAAGGAACTTGAGACTGAAAATATTGTACACGAGCTGTATTCAATATGTGCTGATGTTTCCTTTCAACAGCAGAGTTCTGTTGGGCACATCGACACATGAGTATTGATGTACCACTCCTTTTTTGTTCAAAAAATTCAGTGAATTTCAATTCAGGTGCATTGTCAGATCTCATTTGCTTTATTGTCTTGCCACGTTGAGTCTCGATGAGTTTAAAAAACTGTGGTACAATGAAAACAACATCAGACATGTGTTTGAGCATGAATATCCAAGTGTAATGAGTGGCATCATCAACAATAGTAAGAAAGTAAGGATAACCAGCATGAGTTTGTGTTGAAAATGGTCCCCAAATGTGTACATGAATTAAATCAAATGCATTTGAAGACAAGTGATTATTGGAAGTAAAAGATAACTTCCTTTGTTTTGCCAAAGGACACATTGTACAATGAGATACTTTGTTTACTGTAGAGGATTTCAAATGTAAAAGGTTTTGTAATGAATAAGCAATCAGCAGAGGGATGGCCCAGCTGTTGATTCCATAAGGATGCTTTATTATCAACAATAGCACAAATGGATATATTTGCAGGAAAGTCTGGTCAAGAATGTACAGTCCTTGGAAAAGTTTAGCACTGCCAATCCTTTCTAAGTGTACTTGTCCTAAATGATGCAAGTGTTAGTAGAAAATTGAACAAAAACTGATGAATCCTATGTAATGGCACTCATAGAGAGTAGATTGAAAAATATCAGGCATATATAGAACATTTCTCAAAATAAGTGTGTCAGATAATTTCACAGTATCAGTGCTTTTGACCTTATTGGTGTTTTTTTTTTGGCGAACTAATGGATGCAGAACATGGTTCTCTGTCAGTAAGGAATTCTTCAAAACAAGATATATGTGTGGAAGCTCCTGAGTCAATTATCTAATGAGGCAGAGAGAGTACCTACTATGTGAGTGGTAGAACCACTTGAAGAGGCAGCGAATTGAGCTTGAAGCTGGTTAAGGAGGTTTTGGCACTGTAATAGGGCCTCTGTTGGATTTGTGATGTTGTTCAGATTGGGAGAGAACTCATCGGTCAGTGTTATTACCGAATTTGTACTGTTGGTGGAGGAAGTGCTCGAGTTACTGTTGTTGAGTCGCTGCTGTTTGGGCTTATATTCAGGAAGATAGCCATGGACTTTGTAACACTTATCATTGGTATGTCCTGAGATGTTACAATGAGTGCAAATAGGCCGATCTTTGCGTTGTATATGTGCGGAGTCGTTCTTCGCATTGTTTGAAGAGATAGCAAGTGCAGTGGCTGGAGCAACAGAAGAAAAAGAACCTATTGCTTGTTGTTGTCCTTCTTGAAGAAGAAGGGAGAATGCTCGAGAAACAGAAGGAATAGGTTCTATGAGGAGGAGTTGAGCACGGGCTTGAGCAGAAGACTCATCAAGTCCCATGAGTAAGTCCATAAGGTATCCAATTTAAAGAAAACTAGCAATCTCTTGATTTCCTTCACATCTGCAATTTCCACAAGTACAAGCAGGCCTGTTGAAGTTTAATTCATCAACGAGAGTTTTAAACTTTGTGAAATACATACCCACATAGTCTTGATTTTGAGTGAGGGTTGCGAGAGATCTCTTCAACTGGAAAATTCGAGGAGCATTCTTTCCCTAAAAACGCTCTTTGAGATCAAGCCATATACTTCTTGTCAAATCTGCAAAGAGGATGCTGGCTGAAATCGACTTGGATACTGAGTTCAAGATCCAGGATATGACAATGTTGTTGTTTCTGATCCATGAGGGAAGGCATTTGGCCGAGGAAGAGTTCCATCAACAAATCCGAGCTTGTTTTTGACTGATATTCCCATGATTATTGAACGACTCCAAGAAACATAGCTTTCCTCTATTAACGACTCAATGACTAACACTGAACTTGTGTTGTCATTGTGGTGCAGATACTATGGATTGAGATATTCTTGATTCTGATCAATTGAATGTTGAGGGCCATCAGATGTAGGAGGAACGTTTTCTTGAGGTTTAGACAAGGTGGGCGGAATCGGATATGCAGTTTGGGGGTTCTTGGTAGGCATGGTGGTTTTGGTAGGGGAAGGTGGTTGAGAGGGATCGAAGGAAGGAGAAGTCACTCAGGAGGGAAAATATGCTCTGATACCATATTGGAAGCTAAAAAGAATTATAAATGACTTTCTCACTTTATTGAATGGCTGCCCAATGATATATAAGTACCAATGATAAGAGATTGTTTAACTAACTAACAAATTTTAAATCTCTTAACTAATTGTTTAATAACTTCCCACTTGATGAACATCGATCCAGAATTGTGTTGCCATTATTGACAGGCTGTTGATACGGCAGTGAGAATTCTGAAGGAAGGGGGAATGGATGCAATAAAATTGGAAGGGGGTGCACATTCTAGAATAACAGCAGCAAGAGCTATAGTCGAAGCAGGGATTGCGGTAATGGGGCATGTTGGACTTACTCCACAAGCAATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAATATCGTCAGCGCTGTTAAGGTACCTCCACTCCTCTACTTATCTCTTCCTCTTATTGTACTGTTTGCAGCTATTTCTTGGACACCATTCTGTGGTTATGTTGCACAGTTTTTCGTGTTCATTTTGGCTTTTATGCTGAATAGCATCTTTGCAGGTGGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTTGCTGTCGTCCTAGAATGTGTTCCTCCAGAAGTTGCTGCTGCGGCTACAGCTGCTCTCCGTATTCCCACCATGGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAGTTAGTTGATTGCACATCCATCCTTATATATGCCATCTGTACATACGTACATGTATATATATAAATAAATATATGACCGCTAACTATATTTCCTTTCCTTGAGCTGTTTGTTTGTTTCTCGTTTGATCTGGCATATTATTAATAACTTCAAATTATTTCTTTACCTAGTTGAGGATTTGGAGGTTCTCTCCTGAATGAGAGAAGCATCGAAGGTTGTCCCTGTGGTCCTCTTTTATGGTTTTCAAGTCCTAGCACAATGTAATTGGAAGACAATGGCTGTAACTATTTTAACTGTTTCAAGATTCCCTAAAGGGTGTGGATGCATCAGAATATAGAAGACATTCAAGAATAGTGATTTTTTTTTTCCGGTCAACAGTACACTCACTAACAAGTGGAAATGAAATAACAATTTGATCACATTTGGGTTTGAATGCCGCTCTCGTATTGAAAGGAGAGTGTGAAGAAGAAAATGGACATTGCATTCATTCAATTAGGAGAGTCAGGGTTTATGATTTATTAAAATATTTCAGACAACTTGTTGGATTTTTATTTTTTGCTTTTTTTTAAATAGAAATGTCAGTTTCATCTTCCATACTTGTTTGATAAGGTTGATCGGTGTGTTCTCTTGTTGTACTTGAGAACTTGACAACAATCATAGAGTCGAGTCGAATGCCCTTTGCAGTTGTACTAATTAATTTTCTTAAACTCAGGTGCTAGTTTACCATGATCTTCTCGGCATGCTTCAACACCCTCATCATGCTAAGGTTACCATCAGGCTGCACTTTTGTTTCGTATAGGTTTCTCCATGTTTCTTTATGATGTTGCGACTCCTTTTGTTTATGCCCTTTGCAGGTGACCCCGAAATTCTGTAAGCAATTTGCTCAAATAGGAGATGTCATCAACAAAGCTTTATTAGAATACAGGGAAGAAGTATCTAATCGTTCGTTTCCTGGACCGCACCATAGCCCATATAAGATCAGTGACCCTGATCTGAATGGTTTCTTGAAAGAGTTACAAAATATGGGATTGGATGAGGCAGCATCAGCAGCTGCTGCTAAAGCCAACCGATGATTGAACTCAGGCCACATTTAATTGGCCCGCCTTCCTTGGTCTTGCTTTTTTTTGAGTTTTAAATAATTCAGGGAATTTATAAGGTGGATCAATTTGTTCAAAGAATCAGTAGCTGGAATGCTTCAAACTCTTGTGATCGCAATCAACGTTTTCTTTAGGCAACCAGTTCCTTCTTAGATTTCCTCAATGATGAGCCCTTTCTTTTAGTTAGCTGATATGAAGATGTCTTTGGATTCTGAACAAAATGAAGATATCCAAAGAAAACGGAAAAGGAAGCAAGGCCACAGTCGCATGATGATTCTTTTTCTAACTAAATATTTTAGTGGTGGAATAAGCAAGCAAAAAAAGATATACCTATCTAAACTTAAGTTGTATTTCTTGCATGCAATAAATGCATGCTCCAATAGCTATAGAACAGTACTTAAATGTAATTTAAGTAGCAATGAATGGACCTGAAATAAAAAAAAGATATATCAAAGATAATTAACATGTTTTTTTA

mRNA sequence

ATATTCCGTACTTAAGCCACTCAGTACTTGATGCTCTACGAAGCATGCTCTCAGGCTTCAACCATTTGTTCGTGATTTAGTTGGAGTAACGTTGGTGAGCAATCGATCAATGGCGCTATTCAAGCTTTTAACTATGGCCGCATCTCGAACAAGAAAACAATCCCTCCTCAAAACCCTTCGTTACATGAGCAATGTCCCTGAAAATACGGTCTACAGCGGCCCCACCTCCCAAACTCCTAATCAAAGAGTCACTCTCACCAATCTTAGACAAAAGTACAAGAAAGGTGAACCCATCACCATGGTCACTGCTTACGATTACCCTTCCGGTGTCCATTTGGACATGGCTGGGATTGATATTGCTTTGGTTGGCGATTCGGCTGCCATGGTTGTGCACGGCCACGACACTACGTTACCGATTAGTTTGGATGAAATCCTTGTTCATTGTAGGGCTGTTGCTCGTGGGGCTAGGAGACCTCTTTTGATTGGTGATTTGCCGTTTGGTACTTATGAATCCAGCTCTGCGCAGGTGTTTGAAGAAATGACAAAGTTAGAGAATATTGGGAGAGAACTCATCGGTCAGTGTTATTACCGAATTTGTACTGTTGGTGGAGGAAGTGCTCGAGTTACTGTTGTTGAGTCGCTGCTGTTTGGGCTTATATTCAGGAAGATAGCCATGGACTTTGCTGTTGATACGGCAGTGAGAATTCTGAAGGAAGGGGGAATGGATGCAATAAAATTGGAAGGGGGTGCACATTCTAGAATAACAGCAGCAAGAGCTATAGTCGAAGCAGGGATTGCGGTAATGGGGCATGTTGGACTTACTCCACAAGCAATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAATATCGTCAGCGCTGTTAAGGTGGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTTGCTGTCGTCCTAGAATGTGTTCCTCCAGAAGTTGCTGCTGCGGCTACAGCTGCTCTCCGTATTCCCACCATGGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAAAATGTCAGTTTCATCTTCCATACTTGTTTGATAAGGTGCTAGTTTACCATGATCTTCTCGGCATGCTTCAACACCCTCATCATGCTAAGGTGACCCCGAAATTCTGTAAGCAATTTGCTCAAATAGGAGATGTCATCAACAAAGCTTTATTAGAATACAGGGAAGAAGTATCTAATCGTTCGTTTCCTGGACCGCACCATAGCCCATATAAGATCAGTGACCCTGATCTGAATGGTTTCTTGAAAGAGTTACAAAATATGGGATTGGATGAGGCAGCATCAGCAGCTGCTGCTAAAGCCAACCGATGATTGAACTCAGGCCACATTTAATTGGCCCGCCTTCCTTGGTCTTGCTTTTTTTTGAGTTTTAAATAATTCAGGGAATTTATAAGGTGGATCAATTTGTTCAAAGAATCAGTAGCTGGAATGCTTCAAACTCTTGTGATCGCAATCAACGTTTTCTTTAGGCAACCAGTTCCTTCTTAGATTTCCTCAATGATGAGCCCTTTCTTTTAGTTAGCTGATATGAAGATGTCTTTGGATTCTGAACAAAATGAAGATATCCAAAGAAAACGGAAAAGGAAGCAAGGCCACAGTCGCATGATGATTCTTTTTCTAACTAAATATTTTAGTGGTGGAATAAGCAAGCAAAAAAAGATATACCTATCTAAACTTAAGTTGTATTTCTTGCATGCAATAAATGCATGCTCCAATAGCTATAGAACAGTACTTAAATGTAATTTAAGTAGCAATGAATGGACCTGAAATAAAAAAAAGATATATCAAAGATAATTAACATGTTTTTTTA

Coding sequence (CDS)

ATGGCGCTATTCAAGCTTTTAACTATGGCCGCATCTCGAACAAGAAAACAATCCCTCCTCAAAACCCTTCGTTACATGAGCAATGTCCCTGAAAATACGGTCTACAGCGGCCCCACCTCCCAAACTCCTAATCAAAGAGTCACTCTCACCAATCTTAGACAAAAGTACAAGAAAGGTGAACCCATCACCATGGTCACTGCTTACGATTACCCTTCCGGTGTCCATTTGGACATGGCTGGGATTGATATTGCTTTGGTTGGCGATTCGGCTGCCATGGTTGTGCACGGCCACGACACTACGTTACCGATTAGTTTGGATGAAATCCTTGTTCATTGTAGGGCTGTTGCTCGTGGGGCTAGGAGACCTCTTTTGATTGGTGATTTGCCGTTTGGTACTTATGAATCCAGCTCTGCGCAGGTGTTTGAAGAAATGACAAAGTTAGAGAATATTGGGAGAGAACTCATCGGTCAGTGTTATTACCGAATTTGTACTGTTGGTGGAGGAAGTGCTCGAGTTACTGTTGTTGAGTCGCTGCTGTTTGGGCTTATATTCAGGAAGATAGCCATGGACTTTGCTGTTGATACGGCAGTGAGAATTCTGAAGGAAGGGGGAATGGATGCAATAAAATTGGAAGGGGGTGCACATTCTAGAATAACAGCAGCAAGAGCTATAGTCGAAGCAGGGATTGCGGTAATGGGGCATGTTGGACTTACTCCACAAGCAATTAGTGTTCTTGGAGGATTTAGACCTCAAGGGAAGAATATCGTCAGCGCTGTTAAGGTGGTGGAAACTGCAATCTCTCTACAAGAAGCTGGGTGTTTTGCTGTCGTCCTAGAATGTGTTCCTCCAGAAGTTGCTGCTGCGGCTACAGCTGCTCTCCGTATTCCCACCATGGGCATTGGAGCAGGGCCTTTCTGTAGTGGACAAAAATGTCAGTTTCATCTTCCATACTTGTTTGATAAGGTGCTAGTTTACCATGATCTTCTCGGCATGCTTCAACACCCTCATCATGCTAAGGTGACCCCGAAATTCTGTAAGCAATTTGCTCAAATAGGAGATGTCATCAACAAAGCTTTATTAGAATACAGGGAAGAAGTATCTAATCGTTCGTTTCCTGGACCGCACCATAGCCCATATAAGATCAGTGACCCTGATCTGAATGGTTTCTTGAAAGAGTTACAAAATATGGGATTGGATGAGGCAGCATCAGCAGCTGCTGCTAAAGCCAACCGATGA

Protein sequence

MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLFGLIFRKIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPFCSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR
Homology
BLAST of MELO3C012767 vs. NCBI nr
Match: XP_008447278.1 (PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucumis melo])

HSP 1 Score: 642.5 bits (1656), Expect = 2.5e-180
Identity = 347/411 (84.43%), Postives = 347/411 (84.43%), Query Frame = 0

Query: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLF 180
           RPLLIGDLPFGTYESSSAQ                                         
Sbjct: 121 RPLLIGDLPFGTYESSSAQ----------------------------------------- 180

Query: 181 GLIFRKIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240
                      AVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ
Sbjct: 181 -----------AVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240

Query: 241 AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300
           AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI
Sbjct: 241 AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300

Query: 301 GAGPFCSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 360
           GAGPFCSGQ            VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL
Sbjct: 301 GAGPFCSGQ------------VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 347

Query: 361 EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 412
           EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR
Sbjct: 361 EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 347

BLAST of MELO3C012767 vs. NCBI nr
Match: XP_004139651.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativus] >KGN44587.1 hypothetical protein Csa_016545 [Cucumis sativus])

HSP 1 Score: 621.3 bits (1601), Expect = 5.9e-174
Identity = 336/411 (81.75%), Postives = 339/411 (82.48%), Query Frame = 0

Query: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALFKLLTMA SRTR QSLLKTLRYMSNVPENTVY GPTSQTPNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPI+LDEILVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLF 180
           RPLLIGDLPFGTYESSSAQ                                         
Sbjct: 121 RPLLIGDLPFGTYESSSAQ----------------------------------------- 180

Query: 181 GLIFRKIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240
                      A+DTAVRILKEGGMDAIKLEGG+HSRITAARAIVEAGIAVMGHVGLTPQ
Sbjct: 181 -----------AIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVMGHVGLTPQ 240

Query: 241 AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300
           AISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI
Sbjct: 241 AISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300

Query: 301 GAGPFCSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 360
           GAGPFCSGQ            VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL
Sbjct: 301 GAGPFCSGQ------------VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 347

Query: 361 EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 412
           EYREEVSN SFPGP HSPYKISD D NGFLKELQNMGLDEAASAAAAKANR
Sbjct: 361 EYREEVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

BLAST of MELO3C012767 vs. NCBI nr
Match: XP_038898896.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 597.4 bits (1539), Expect = 9.1e-167
Identity = 323/411 (78.59%), Postives = 331/411 (80.54%), Query Frame = 0

Query: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60
           MA+F L + AASRTR + LLKTLR MSNVPEN VY GPTSQTPN RVTLTNLRQKYKKGE
Sbjct: 1   MAIFNLFSKAASRTRTRPLLKTLRNMSNVPENAVYGGPTSQTPNHRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHG+DTTLPISLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGYDTTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLF 180
           RPLL+GDLPFGTYESSS Q                                         
Sbjct: 121 RPLLVGDLPFGTYESSSVQ----------------------------------------- 180

Query: 181 GLIFRKIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240
                      AVDTAVR+LKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ
Sbjct: 181 -----------AVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240

Query: 241 AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300
           AISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAAT+ALRIPTMGI
Sbjct: 241 AISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATSALRIPTMGI 300

Query: 301 GAGPFCSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 360
           GAGPFCSGQ            VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL 
Sbjct: 301 GAGPFCSGQ------------VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALS 347

Query: 361 EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 412
           EYREEVSN SFPGP HSPYKISD D NGFLKELQNMGLDEAASAAAAKANR
Sbjct: 361 EYREEVSNGSFPGPLHSPYKISDTDFNGFLKELQNMGLDEAASAAAAKANR 347

BLAST of MELO3C012767 vs. NCBI nr
Match: XP_022991968.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 589.3 bits (1518), Expect = 2.5e-164
Identity = 318/411 (77.37%), Postives = 331/411 (80.54%), Query Frame = 0

Query: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALF L+T AA R+  QSLL TLRYMSNVPENTVY GPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLF 180
           RPLLIGDLPFGTYESSS Q                                         
Sbjct: 121 RPLLIGDLPFGTYESSSVQ----------------------------------------- 180

Query: 181 GLIFRKIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240
                      AVDTAVR+LKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ
Sbjct: 181 -----------AVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240

Query: 241 AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300
           AISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPT+GI
Sbjct: 241 AISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGI 300

Query: 301 GAGPFCSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 360
           GAGPFCSGQ            VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL 
Sbjct: 301 GAGPFCSGQ------------VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALS 347

Query: 361 EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 412
           +Y+EEVSN SFPGP HSPYKISD D NGFLKEL++MGLD AASAAAAKAN+
Sbjct: 361 DYKEEVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 347

BLAST of MELO3C012767 vs. NCBI nr
Match: KAG6575974.1 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 586.6 bits (1511), Expect = 1.6e-163
Identity = 317/411 (77.13%), Postives = 330/411 (80.29%), Query Frame = 0

Query: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALF L+T AA R+  QSLL TLR MSNVPENTVY GPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 117 MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 176

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCR+VARGAR
Sbjct: 177 PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 236

Query: 121 RPLLIGDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLF 180
           RPLLIGDLPFGTYESSS Q                                         
Sbjct: 237 RPLLIGDLPFGTYESSSVQ----------------------------------------- 296

Query: 181 GLIFRKIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240
                      AVDTAVR+LKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ
Sbjct: 297 -----------AVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 356

Query: 241 AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300
           AISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPT+GI
Sbjct: 357 AISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGI 416

Query: 301 GAGPFCSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 360
           GAGPFCSGQ            VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL 
Sbjct: 417 GAGPFCSGQ------------VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALS 463

Query: 361 EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 412
           +Y+EEVSN SFPGP HSPYKISD D NGFLKEL++MGLD AASAAAAKANR
Sbjct: 477 DYKEEVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 463

BLAST of MELO3C012767 vs. ExPASy Swiss-Prot
Match: Q9M315 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT2 PE=1 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 7.8e-145
Identity = 271/398 (68.09%), Postives = 304/398 (76.38%), Query Frame = 0

Query: 14  TRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSG 73
           +R    + T+R+MSNVPE+TVY GP  Q  NQRVTLT LRQK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTY 133
           VH+D AGID+ LVGDSAAMVVHG+DTTLPISL+E+LVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLFGLIFRKIAMDFAV 193
           ESS+ Q                                                    AV
Sbjct: 131 ESSTNQ----------------------------------------------------AV 190

Query: 194 DTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGK 253
           DTAVR+LKEGGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVLGGFRPQGK
Sbjct: 191 DTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVLGGFRPQGK 250

Query: 254 NIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPFCSGQKCQF 313
           NI SAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPT+GIGAGPFCSGQ    
Sbjct: 251 NIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPFCSGQ---- 310

Query: 314 HLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNRSFPG 373
                   VLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VINKALLEY+EEVS   FPG
Sbjct: 311 --------VLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPG 344

Query: 374 PHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 412
           P HSPYKIS  DL+GFL ELQ +GLD+AAS AAA A +
Sbjct: 371 PSHSPYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

BLAST of MELO3C012767 vs. ExPASy Swiss-Prot
Match: O82357 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KPHMT1 PE=1 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 3.9e-144
Identity = 271/404 (67.08%), Postives = 310/404 (76.73%), Query Frame = 0

Query: 9   MAASRTRKQSLLK---TLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMV 68
           MA+S TR  S      ++R+MSN+PENTVY GP  Q PNQRVTLT+LRQK+++GEPIT+V
Sbjct: 1   MASSLTRNCSRFSKAISVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVV 60

Query: 69  TAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLI 128
           TAYDYPS VHLD AGID+ LVGDSA+MVVHGHDTTLPISLDE+LVHCRAVARGA+RPLL+
Sbjct: 61  TAYDYPSAVHLDTAGIDVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLV 120

Query: 129 GDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLFGLIFR 188
           GDLPFGTYESSS+Q                                              
Sbjct: 121 GDLPFGTYESSSSQ---------------------------------------------- 180

Query: 189 KIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVL 248
                 AVDTAVR+LKEGGMDAIKLEGG+ SRITAA+AIVEAGIAV+GHVGLTPQAISVL
Sbjct: 181 ------AVDTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGLTPQAISVL 240

Query: 249 GGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPF 308
           GGFRPQG+NI SAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL+IPT+GIGAGPF
Sbjct: 241 GGFRPQGRNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPTIGIGAGPF 300

Query: 309 CSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREE 368
           CSGQ            VLVYHDLLGM+QHPHHAKVTPKFCKQ+A +G+VINKAL+EY+EE
Sbjct: 301 CSGQ------------VLVYHDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEE 340

Query: 369 VSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKA 410
           VS + FPGP HSPYKI+  +L+GFL ELQ +G D+AASAAA  A
Sbjct: 361 VSKKVFPGPSHSPYKITASELDGFLTELQKLGFDKAASAAALAA 340

BLAST of MELO3C012767 vs. ExPASy Swiss-Prot
Match: Q9AWZ8 (3-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT1 PE=2 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 2.1e-129
Identity = 251/398 (63.07%), Postives = 285/398 (71.61%), Query Frame = 0

Query: 24  RYMSNVPENTVYSGPTSQT------------PNQRVTLTNLRQKYKKGEPITMVTAYDYP 83
           R +S+VPE+ VY GP  Q               +RVT+T LR K+++GEPIT+VTAYDYP
Sbjct: 18  RPLSHVPESAVYGGPRPQDVGAAAGAGAGAGATRRVTVTTLRGKHRRGEPITVVTAYDYP 77

Query: 84  SGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFG 143
           S VH+D AGID+ LVGDSAAMVVHGHDTTLPISLD +L HCRAVARGA RPLL+GDLPFG
Sbjct: 78  SAVHVDSAGIDVCLVGDSAAMVVHGHDTTLPISLDVMLEHCRAVARGATRPLLVGDLPFG 137

Query: 144 TYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLFGLIFRKIAMDF 203
            YESSS +                                                    
Sbjct: 138 CYESSSTR---------------------------------------------------- 197

Query: 204 AVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQ 263
           AVD+AVR+LKEGGMDAIKLEGGA SRI+AA+AIVEAGIAVMGHVGLTPQAISVLGGFRPQ
Sbjct: 198 AVDSAVRVLKEGGMDAIKLEGGAPSRISAAKAIVEAGIAVMGHVGLTPQAISVLGGFRPQ 257

Query: 264 GKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPFCSGQKC 323
           GK + SAVKVVETA++LQEAGCF+VVLECVP  VAAAAT+AL+IPT+GIGAGPFCSGQ  
Sbjct: 258 GKTVDSAVKVVETALALQEAGCFSVVLECVPAPVAAAATSALQIPTIGIGAGPFCSGQ-- 317

Query: 324 QFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNRSF 383
                     VLVYHDLLGM+QHPHHAKVTPKFCKQF  +G VINKAL EY++EV  RSF
Sbjct: 318 ----------VLVYHDLLGMMQHPHHAKVTPKFCKQFGNVGHVINKALSEYKQEVETRSF 351

Query: 384 PGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKA 410
           PGP H+PYKI+  D++GF   LQ MGLDEAA+AAAA A
Sbjct: 378 PGPSHTPYKIAAADVDGFANALQKMGLDEAANAAAAAA 351

BLAST of MELO3C012767 vs. ExPASy Swiss-Prot
Match: Q9AWZ7 (3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=KPHMT2 PE=3 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 4.8e-110
Identity = 221/390 (56.67%), Postives = 264/390 (67.69%), Query Frame = 0

Query: 24  RYMSN-VPENTVYSG------PTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSGVHL 83
           R MSN   E  +Y G        S    +RVTL  LR K+++GEPI+MVTAYDYPSGVH+
Sbjct: 54  RAMSNGAAEPAIYGGGGGAQQAASSAAARRVTLATLRGKHRRGEPISMVTAYDYPSGVHV 113

Query: 84  DMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTYESS 143
           D AG DI LVGDSAAMV HGHD TLPISLD ++ HCRAVARGA R  L+GDLPFG+YE+S
Sbjct: 114 DAAGFDICLVGDSAAMVAHGHDNTLPISLDLMIEHCRAVARGAARTFLVGDLPFGSYEAS 173

Query: 144 SAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLFGLIFRKIAMDFAVDTA 203
           +AQ                                                    AV +A
Sbjct: 174 TAQ----------------------------------------------------AVGSA 233

Query: 204 VRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGKNIV 263
           VR++KEGG+++IKLEG A SRI+AARAIV+AGIAVMGH+GLTPQ++S LGGFRPQGK + 
Sbjct: 234 VRVMKEGGVNSIKLEGSAPSRISAARAIVDAGIAVMGHIGLTPQSVSALGGFRPQGKTVE 293

Query: 264 SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPFCSGQKCQFHLP 323
           SAVKVVE A++LQEAGCFAVVLECVP  VAAAAT+AL IPT+GIGAGPFCSGQ       
Sbjct: 294 SAVKVVEAALALQEAGCFAVVLECVPAPVAAAATSALTIPTIGIGAGPFCSGQ------- 353

Query: 324 YLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNRSFPGPHH 383
                VLVYHDLLG  Q   HAKV+PKFCKQ+  IGDVIN+AL +Y++EV  +SFPGP H
Sbjct: 354 -----VLVYHDLLGTFQ-TSHAKVSPKFCKQYGNIGDVINRALSKYKQEVETQSFPGPSH 378

Query: 384 SPYKISDPDLNGFLKELQNMGLDEAASAAA 407
           +PYK++  D++ FL  L+  GL+ AA AAA
Sbjct: 414 TPYKLAATDVDAFLNALKMKGLNVAADAAA 378

BLAST of MELO3C012767 vs. ExPASy Swiss-Prot
Match: B0TC10 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum (strain ATCC 51547 / Ice1) OX=498761 GN=panB PE=3 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 2.3e-59
Identity = 131/333 (39.34%), Postives = 191/333 (57.36%), Query Frame = 0

Query: 45  QRVTLTNLRQKYKKGEPITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPIS 104
           +++TL   ++  K+G+ + M+TAYDYP    +D + I+I LVGDS  MVV G+D+T+P++
Sbjct: 3   KKITLPQCKEMKKQGKRLRMITAYDYPFARLVDESEIEIILVGDSLGMVVLGYDSTVPVT 62

Query: 105 LDEILVHCRAVARGARRPLLIGDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICT 164
           LDE++ HC+ V RGA   L++ D+PFG+Y                               
Sbjct: 63  LDEMIHHCKPVVRGAPNTLVVADMPFGSY------------------------------- 122

Query: 165 VGGGSARVTVVESLLFGLIFRKIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAI 224
                                 ++ + A+  A R+LKE G++A+K+EGG     T  RA+
Sbjct: 123 ---------------------NVSKEDAIRNANRMLKESGIEAVKVEGGTRMAPT-VRAL 182

Query: 225 VEAGIAVMGHVGLTPQAISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPE 284
           V+AGI VMGH+GLTPQ  + LGGF+ QGK   +A +++E A++L+ AG F++V+ECVP  
Sbjct: 183 VDAGIPVMGHIGLTPQTAAQLGGFKVQGKTEDAAQQLLEDALALESAGAFSIVIECVPAG 242

Query: 285 VAAAATAALRIPTMGIGAGPFCSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKF 344
           +A   TA+L IPT+GIGAGP+C+GQ            VLV  DLLG+     + +  PKF
Sbjct: 243 LARTITASLSIPTIGIGAGPYCNGQ------------VLVIQDLLGI-----YDRFVPKF 265

Query: 345 CKQFAQIGDVINKALLEYREEVSNRSFPGPHHS 378
            KQ+AQ G  I  AL +Y  EV++  FPGP HS
Sbjct: 303 VKQYAQTGPAIRAALNDYAREVADGVFPGPEHS 265

BLAST of MELO3C012767 vs. ExPASy TrEMBL
Match: A0A1S3BH28 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103489751 PE=3 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 1.2e-180
Identity = 347/411 (84.43%), Postives = 347/411 (84.43%), Query Frame = 0

Query: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLF 180
           RPLLIGDLPFGTYESSSAQ                                         
Sbjct: 121 RPLLIGDLPFGTYESSSAQ----------------------------------------- 180

Query: 181 GLIFRKIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240
                      AVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ
Sbjct: 181 -----------AVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240

Query: 241 AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300
           AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI
Sbjct: 241 AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300

Query: 301 GAGPFCSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 360
           GAGPFCSGQ            VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL
Sbjct: 301 GAGPFCSGQ------------VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 347

Query: 361 EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 412
           EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR
Sbjct: 361 EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 347

BLAST of MELO3C012767 vs. ExPASy TrEMBL
Match: A0A0A0K8B2 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G338120 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.9e-174
Identity = 336/411 (81.75%), Postives = 339/411 (82.48%), Query Frame = 0

Query: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALFKLLTMA SRTR QSLLKTLRYMSNVPENTVY GPTSQTPNQRVTLTNLRQKYKKGE
Sbjct: 1   MALFKLLTMAVSRTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLRQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPI+LDEILVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPITLDEILVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLF 180
           RPLLIGDLPFGTYESSSAQ                                         
Sbjct: 121 RPLLIGDLPFGTYESSSAQ----------------------------------------- 180

Query: 181 GLIFRKIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240
                      A+DTAVRILKEGGMDAIKLEGG+HSRITAARAIVEAGIAVMGHVGLTPQ
Sbjct: 181 -----------AIDTAVRILKEGGMDAIKLEGGSHSRITAARAIVEAGIAVMGHVGLTPQ 240

Query: 241 AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300
           AISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI
Sbjct: 241 AISVLGGFRPQGKNITSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300

Query: 301 GAGPFCSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 360
           GAGPFCSGQ            VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL
Sbjct: 301 GAGPFCSGQ------------VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 347

Query: 361 EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 412
           EYREEVSN SFPGP HSPYKISD D NGFLKELQNMGLDEAASAAAAKANR
Sbjct: 361 EYREEVSNGSFPGPLHSPYKISDADFNGFLKELQNMGLDEAASAAAAKANR 347

BLAST of MELO3C012767 vs. ExPASy TrEMBL
Match: A0A6J1JXR6 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111488459 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 1.2e-164
Identity = 318/411 (77.37%), Postives = 331/411 (80.54%), Query Frame = 0

Query: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALF L+T AA R+  QSLL TLRYMSNVPENTVY GPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRYMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLF 180
           RPLLIGDLPFGTYESSS Q                                         
Sbjct: 121 RPLLIGDLPFGTYESSSVQ----------------------------------------- 180

Query: 181 GLIFRKIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240
                      AVDTAVR+LKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ
Sbjct: 181 -----------AVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240

Query: 241 AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300
           AISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPT+GI
Sbjct: 241 AISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGI 300

Query: 301 GAGPFCSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 360
           GAGPFCSGQ            VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL 
Sbjct: 301 GAGPFCSGQ------------VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALS 347

Query: 361 EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 412
           +Y+EEVSN SFPGP HSPYKISD D NGFLKEL++MGLD AASAAAAKAN+
Sbjct: 361 DYKEEVSNGSFPGPLHSPYKISDNDYNGFLKELRSMGLDGAASAAAAKANQ 347

BLAST of MELO3C012767 vs. ExPASy TrEMBL
Match: A0A6J1GNW3 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456114 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 7.8e-164
Identity = 317/411 (77.13%), Postives = 330/411 (80.29%), Query Frame = 0

Query: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60
           MALF L+T AA R+  QSLL TLR MSNVPENTVY GPTS TPNQRVTLTNL+QKYKKGE
Sbjct: 1   MALFNLVTKAAVRSTSQSLLNTLRCMSNVPENTVYGGPTSNTPNQRVTLTNLKQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSA+MVVHGHDTTLPI+LDE+LVHCR+VARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSASMVVHGHDTTLPITLDEMLVHCRSVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLF 180
           RPLLIGDLPFGTYESSS Q                                         
Sbjct: 121 RPLLIGDLPFGTYESSSVQ----------------------------------------- 180

Query: 181 GLIFRKIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240
                      AVDTAVR+LKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ
Sbjct: 181 -----------AVDTAVRVLKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240

Query: 241 AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300
           AISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPT+GI
Sbjct: 241 AISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTIGI 300

Query: 301 GAGPFCSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 360
           GAGPFCSGQ            VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKAL 
Sbjct: 301 GAGPFCSGQ------------VLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALS 347

Query: 361 EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 412
           +Y+EEVSN SFPGP HSPYKISD D NGFLKEL++MGLD AASAAAAKANR
Sbjct: 361 DYKEEVSNGSFPGPLHSPYKISDDDYNGFLKELRSMGLDGAASAAAAKANR 347

BLAST of MELO3C012767 vs. ExPASy TrEMBL
Match: A0A1S4DXV9 (3-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103491726 PE=3 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 5.1e-163
Identity = 316/410 (77.07%), Postives = 330/410 (80.49%), Query Frame = 0

Query: 1   MALFKLLTMAASRTRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGE 60
           MA+FKLLT  AS+TR QSLLKTLRYMSNVPENTVY GPTSQTPNQRVTLTNL QKYKKGE
Sbjct: 1   MAVFKLLTWIASQTRTQSLLKTLRYMSNVPENTVYGGPTSQTPNQRVTLTNLIQKYKKGE 60

Query: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGAR 120
           PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGH++TLPISLDE+LVHCRAVARGAR
Sbjct: 61  PITMVTAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHNSTLPISLDEMLVHCRAVARGAR 120

Query: 121 RPLLIGDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLF 180
           RP LI DLPFGTYESS  Q                                         
Sbjct: 121 RPFLIVDLPFGTYESSHVQ----------------------------------------- 180

Query: 181 GLIFRKIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQ 240
                      AVDTAV+ILKEG MDAIKLEGGAHSRITAARAIV+AGIAVMGHVGLTPQ
Sbjct: 181 -----------AVDTAVKILKEGRMDAIKLEGGAHSRITAARAIVDAGIAVMGHVGLTPQ 240

Query: 241 AISVLGGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGI 300
           AISVLGGFRPQGKNI SAVKVVETAISLQEAGCFAVVLECVPPEVAAAAT ALRIPT+GI
Sbjct: 241 AISVLGGFRPQGKNIASAVKVVETAISLQEAGCFAVVLECVPPEVAAAATVALRIPTLGI 300

Query: 301 GAGPFCSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALL 360
           GAGPFCSGQ            VLVYHDLLG+LQHPHHAKVTPKFCKQFAQIGDVIN+AL 
Sbjct: 301 GAGPFCSGQ------------VLVYHDLLGILQHPHHAKVTPKFCKQFAQIGDVINQALS 346

Query: 361 EYREEVSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKAN 411
           EYREEVSN SFPGP H+PYKISD DLNGF+KELQNMGLDEAASAA+AKA+
Sbjct: 361 EYREEVSNGSFPGPLHNPYKISDADLNGFMKELQNMGLDEAASAASAKAS 346

BLAST of MELO3C012767 vs. TAIR 10
Match: AT3G61530.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 515.0 bits (1325), Expect = 5.6e-146
Identity = 271/398 (68.09%), Postives = 304/398 (76.38%), Query Frame = 0

Query: 14  TRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSG 73
           +R    + T+R+MSNVPE+TVY GP  Q  NQRVTLT LRQK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTY 133
           VH+D AGID+ LVGDSAAMVVHG+DTTLPISL+E+LVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLFGLIFRKIAMDFAV 193
           ESS+ Q                                                    AV
Sbjct: 131 ESSTNQ----------------------------------------------------AV 190

Query: 194 DTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGK 253
           DTAVR+LKEGGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVLGGFRPQGK
Sbjct: 191 DTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVLGGFRPQGK 250

Query: 254 NIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPFCSGQKCQF 313
           NI SAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPT+GIGAGPFCSGQ    
Sbjct: 251 NIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPFCSGQ---- 310

Query: 314 HLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNRSFPG 373
                   VLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VINKALLEY+EEVS   FPG
Sbjct: 311 --------VLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPG 344

Query: 374 PHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 412
           P HSPYKIS  DL+GFL ELQ +GLD+AAS AAA A +
Sbjct: 371 PSHSPYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

BLAST of MELO3C012767 vs. TAIR 10
Match: AT3G61530.2 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 515.0 bits (1325), Expect = 5.6e-146
Identity = 271/398 (68.09%), Postives = 304/398 (76.38%), Query Frame = 0

Query: 14  TRKQSLLKTLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMVTAYDYPSG 73
           +R    + T+R+MSNVPE+TVY GP  Q  NQRVTLT LRQK++KGEPITMVTAYDYPS 
Sbjct: 11  SRASRAITTVRFMSNVPEDTVYGGPKPQNSNQRVTLTQLRQKHRKGEPITMVTAYDYPSA 70

Query: 74  VHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLIGDLPFGTY 133
           VH+D AGID+ LVGDSAAMVVHG+DTTLPISL+E+LVHCRAVARGA+RPLL+GDLPFGTY
Sbjct: 71  VHIDTAGIDVCLVGDSAAMVVHGYDTTLPISLEEMLVHCRAVARGAKRPLLVGDLPFGTY 130

Query: 134 ESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLFGLIFRKIAMDFAV 193
           ESS+ Q                                                    AV
Sbjct: 131 ESSTNQ----------------------------------------------------AV 190

Query: 194 DTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVLGGFRPQGK 253
           DTAVR+LKEGGMDAIKLEGG+ SRITAA++IVEAGIAVMGHVGLTPQAISVLGGFRPQGK
Sbjct: 191 DTAVRVLKEGGMDAIKLEGGSPSRITAAKSIVEAGIAVMGHVGLTPQAISVLGGFRPQGK 250

Query: 254 NIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPFCSGQKCQF 313
           NI SAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL IPT+GIGAGPFCSGQ    
Sbjct: 251 NIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALNIPTIGIGAGPFCSGQ---- 310

Query: 314 HLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREEVSNRSFPG 373
                   VLVYHDLLGM+QHPHHAKVTPKFCKQ+AQ+G+VINKALLEY+EEVS   FPG
Sbjct: 311 --------VLVYHDLLGMMQHPHHAKVTPKFCKQYAQVGEVINKALLEYKEEVSKHLFPG 344

Query: 374 PHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKANR 412
           P HSPYKIS  DL+GFL ELQ +GLD+AAS AAA A +
Sbjct: 371 PSHSPYKISSSDLDGFLSELQKLGLDKAASDAAASAEK 344

BLAST of MELO3C012767 vs. TAIR 10
Match: AT2G46110.1 (ketopantoate hydroxymethyltransferase 1 )

HSP 1 Score: 512.7 bits (1319), Expect = 2.8e-145
Identity = 271/404 (67.08%), Postives = 310/404 (76.73%), Query Frame = 0

Query: 9   MAASRTRKQSLLK---TLRYMSNVPENTVYSGPTSQTPNQRVTLTNLRQKYKKGEPITMV 68
           MA+S TR  S      ++R+MSN+PENTVY GP  Q PNQRVTLT+LRQK+++GEPIT+V
Sbjct: 1   MASSLTRNCSRFSKAISVRFMSNLPENTVYGGPKPQNPNQRVTLTHLRQKHRRGEPITVV 60

Query: 69  TAYDYPSGVHLDMAGIDIALVGDSAAMVVHGHDTTLPISLDEILVHCRAVARGARRPLLI 128
           TAYDYPS VHLD AGID+ LVGDSA+MVVHGHDTTLPISLDE+LVHCRAVARGA+RPLL+
Sbjct: 61  TAYDYPSAVHLDTAGIDVCLVGDSASMVVHGHDTTLPISLDEMLVHCRAVARGAKRPLLV 120

Query: 129 GDLPFGTYESSSAQVFEEMTKLENIGRELIGQCYYRICTVGGGSARVTVVESLLFGLIFR 188
           GDLPFGTYESSS+Q                                              
Sbjct: 121 GDLPFGTYESSSSQ---------------------------------------------- 180

Query: 189 KIAMDFAVDTAVRILKEGGMDAIKLEGGAHSRITAARAIVEAGIAVMGHVGLTPQAISVL 248
                 AVDTAVR+LKEGGMDAIKLEGG+ SRITAA+AIVEAGIAV+GHVGLTPQAISVL
Sbjct: 181 ------AVDTAVRVLKEGGMDAIKLEGGSASRITAAKAIVEAGIAVIGHVGLTPQAISVL 240

Query: 249 GGFRPQGKNIVSAVKVVETAISLQEAGCFAVVLECVPPEVAAAATAALRIPTMGIGAGPF 308
           GGFRPQG+NI SAVKVVETA++LQEAGCF+VVLECVPP VAAAAT+AL+IPT+GIGAGPF
Sbjct: 241 GGFRPQGRNIASAVKVVETAMALQEAGCFSVVLECVPPPVAAAATSALKIPTIGIGAGPF 300

Query: 309 CSGQKCQFHLPYLFDKVLVYHDLLGMLQHPHHAKVTPKFCKQFAQIGDVINKALLEYREE 368
           CSGQ            VLVYHDLLGM+QHPHHAKVTPKFCKQ+A +G+VINKAL+EY+EE
Sbjct: 301 CSGQ------------VLVYHDLLGMMQHPHHAKVTPKFCKQYANVGEVINKALMEYKEE 340

Query: 369 VSNRSFPGPHHSPYKISDPDLNGFLKELQNMGLDEAASAAAAKA 410
           VS + FPGP HSPYKI+  +L+GFL ELQ +G D+AASAAA  A
Sbjct: 361 VSKKVFPGPSHSPYKITASELDGFLTELQKLGFDKAASAAALAA 340

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008447278.12.5e-18084.43PREDICTED: 3-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-lik... [more]
XP_004139651.15.9e-17481.753-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial [Cucumis sativ... [more]
XP_038898896.19.1e-16778.593-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Benincas... [more]
XP_022991968.12.5e-16477.373-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial-like [Cucurbit... [more]
KAG6575974.11.6e-16377.133-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial, partial [Cucu... [more]
Match NameE-valueIdentityDescription
Q9M3157.8e-14568.093-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis... [more]
O823573.9e-14467.083-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis... [more]
Q9AWZ82.1e-12963.073-methyl-2-oxobutanoate hydroxymethyltransferase 1, mitochondrial OS=Oryza sativ... [more]
Q9AWZ74.8e-11056.673-methyl-2-oxobutanoate hydroxymethyltransferase 2, mitochondrial OS=Oryza sativ... [more]
B0TC102.3e-5939.343-methyl-2-oxobutanoate hydroxymethyltransferase OS=Heliobacterium modesticaldum... [more]
Match NameE-valueIdentityDescription
A0A1S3BH281.2e-18084.433-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A0A0K8B22.9e-17481.753-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=C... [more]
A0A6J1JXR61.2e-16477.373-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1GNW37.8e-16477.133-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucurbita moschata OX=3662 G... [more]
A0A1S4DXV95.1e-16377.073-methyl-2-oxobutanoate hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT3G61530.15.6e-14668.09Phosphoenolpyruvate carboxylase family protein [more]
AT3G61530.25.6e-14668.09Phosphoenolpyruvate carboxylase family protein [more]
AT2G46110.12.8e-14567.08ketopantoate hydroxymethyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003700Ketopantoate hydroxymethyltransferasePFAMPF02548Pantoate_transfcoord: 160..373
e-value: 6.6E-58
score: 196.1
coord: 46..147
e-value: 2.2E-34
score: 119.0
IPR003700Ketopantoate hydroxymethyltransferasePANTHERPTHR208813-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 164..410
coord: 19..147
IPR003700Ketopantoate hydroxymethyltransferaseHAMAPMF_00156PanBcoord: 47..378
score: 34.989384
IPR003700Ketopantoate hydroxymethyltransferaseCDDcd06557KPHMT-likecoord: 49..372
e-value: 3.87137E-128
score: 367.9
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 157..387
e-value: 5.2E-64
score: 218.1
coord: 44..156
e-value: 1.1E-40
score: 141.6
NoneNo IPR availablePIRSRPIRSR000388-3PIRSR000388-3coord: 158..373
e-value: 3.8E-47
score: 158.6
NoneNo IPR availablePANTHERPTHR20881:SF13-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 19..147
NoneNo IPR availablePANTHERPTHR20881:SF13-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASEcoord: 164..410
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 46..147
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 159..377

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C012767.1MELO3C012767.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015940 pantothenate biosynthetic process
molecular_function GO:0003864 3-methyl-2-oxobutanoate hydroxymethyltransferase activity
molecular_function GO:0003824 catalytic activity