MELO3C009858 (gene) Melon (DHL92) v4

Overview


Name	MELO3C009858
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	Calcium-binding EF hand family protein
Location	chr04: 27568463 .. 27569218 (+)
RNA-Seq Expression	MELO3C009858
Synteny	MELO3C009858

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCAATTCACCACATAACTCAGTCCCCAAACAAAATTCTATATCCATGTTCTTTAGATTAGAGTACCATTTAATTACAATGCAAACGCAGGTAACCAACGCTGTCAAGAGCGTGCAGCTCGACCCGTCACCGCTCGTTTTTGTCGATTGGATATCAAATCTACCAATTTTATTCAACTTTGCAGGCAGAATACATGAGCTGATTAGATCTCTACCTTATTGGTCAATTCTTCAATCACAGTACTCCAAAGAGAAAGCTTTATCACAAAGTAATTGCTCTTCACAATCTGATGATAAAATCATCATCACTACTCAACAAAATTGCTCAATGAGCAGAGAGGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGGAGGGAAGAAAATGATGATGATGATGATGATGAAGAAATTGGGGCAAGTGATGAAATTATAAGAGGTATGTTTGAAGAGAATGAACCAAGTTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAAAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATAGCCAGATTTGATCTCAATAATGATGGTAAAATTGATTTCCATGAATTTGTTAAGTTTATGGAAGTTGCTTTGTGTTAGAATATTTTGCTTCTACTATTATAATTATTTGTAATAACTTTTTTCATTTTTATTACAGACATTGTTCTTGAA

mRNA sequence

TCCAATTCACCACATAACTCAGTCCCCAAACAAAATTCTATATCCATGTTCTTTAGATTAGAGTACCATTTAATTACAATGCAAACGCAGGTAACCAACGCTGTCAAGAGCGTGCAGCTCGACCCGTCACCGCTCGTTTTTGTCGATTGGATATCAAATCTACCAATTTTATTCAACTTTGCAGGCAGAATACATGAGCTGATTAGATCTCTACCTTATTGGTCAATTCTTCAATCACAGTACTCCAAAGAGAAAGCTTTATCACAAAGTAATTGCTCTTCACAATCTGATGATAAAATCATCATCACTACTCAACAAAATTGCTCAATGAGCAGAGAGGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGGAGGGAAGAAAATGATGATGATGATGATGATGAAGAAATTGGGGCAAGTGATGAAATTATAAGAGGTATGTTTGAAGAGAATGAACCAAGTTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAAAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATAGCCAGATTTGATCTCAATAATGATGGTAAAATTGATTTCCATGAATTTGTTAAGTTTATGGAAGTTGCTTTGTGTTAGAATATTTTGCTTCTACTATTATAATTATTTGTAATAACTTTTTTCATTTTTATTACAGACATTGTTCTTGAA

Coding sequence (CDS)

ATGTTCTTTAGATTAGAGTACCATTTAATTACAATGCAAACGCAGGTAACCAACGCTGTCAAGAGCGTGCAGCTCGACCCGTCACCGCTCGTTTTTGTCGATTGGATATCAAATCTACCAATTTTATTCAACTTTGCAGGCAGAATACATGAGCTGATTAGATCTCTACCTTATTGGTCAATTCTTCAATCACAGTACTCCAAAGAGAAAGCTTTATCACAAAGTAATTGCTCTTCACAATCTGATGATAAAATCATCATCACTACTCAACAAAATTGCTCAATGAGCAGAGAGGAAGTGAGATTTGTGATGGAAAAATTAGAGCTTTTTTGGAGGGAAGAAAATGATGATGATGATGATGATGAAGAAATTGGGGCAAGTGATGAAATTATAAGAGGTATGTTTGAAGAGAATGAACCAAGTTTGGAAGAATTGAAGCAAACATTTAATGTGTTTGATAGAAACAAAGATGGTTTCATTGATGAACATGAATTGTTCATAGTTCTGAGCCTGCTTGAATCTAATAAAGGCATTTTCATCCATGATTGCAAAACAATGATAGCCAGATTTGATCTCAATAATGATGGTAAAATTGATTTCCATGAATTTGTTAAGTTTATGGAAGTTGCTTTGTGTTAG

Protein sequence

MFFRLEYHLITMQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALC

Homology

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BLAST of MELO3C009858 vs. NCBI nr
Match: XP_008443616.1 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 399.8 bits (1026), Expect = 1.4e-107
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 12  MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 71
           MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 72  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII 131
           LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII 120

Query: 132 RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 191
           RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD
Sbjct: 121 RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 180

Query: 192 LNNDGKIDFHEFVKFMEVALC 213
           LNNDGKIDFHEFVKFMEVALC
Sbjct: 181 LNNDGKIDFHEFVKFMEVALC 201

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BLAST of MELO3C009858 vs. NCBI nr
Match: KGN59556.1 (hypothetical protein Csa_001548 [Cucumis sativus])

HSP 1 Score: 333.6 bits (854), Expect = 1.3e-87
Identity = 179/212 (84.43%), Postives = 192/212 (90.57%), Query Frame = 0


Query: 1   MFFRLEYHLITMQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWS 60
           MFFRLEY+LITM+T++T+ +KSVQLDPSP VFVD IS LPILFNFA RI EL+RSLP W 
Sbjct: 1   MFFRLEYYLITMETRLTD-IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLP-WP 60

Query: 61  ILQSQYSKEKALSQSNCSSQSDDKIIITTQQNC-SMSREEVRFVMEKLELFWREENDDDD 120
           ILQ  +SKEKALSQ NCSS+S D+IIITTQQNC SMSREEVRFVMEKLELFWREE D   
Sbjct: 61  ILQ-PHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGG 120

Query: 121 DDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIF 180
            D+ IG SDE+IRGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNKGIF
Sbjct: 121 GDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIF 180

Query: 181 IHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 212
           IHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL
Sbjct: 181 IHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 209

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BLAST of MELO3C009858 vs. NCBI nr
Match: XP_004147503.1 (probable calcium-binding protein CML45 [Cucumis sativus])

HSP 1 Score: 313.9 bits (803), Expect = 1.0e-81
Identity = 169/201 (84.08%), Postives = 181/201 (90.05%), Query Frame = 0


Query: 12  MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 71
           M+T++T+ +KSVQLDPSP VFVD IS LPILFNFA RI EL+RSLP W ILQ  +SKEKA
Sbjct: 1   METRLTD-IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLP-WPILQ-PHSKEKA 60

Query: 72  LSQSNCSSQSDDKIIITTQQNC-SMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEI 131
           LSQ NCSS+S D+IIITTQQNC SMSREEVRFVMEKLELFWREE D    D+ IG SDE+
Sbjct: 61  LSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEM 120

Query: 132 IRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 191
           IRGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF
Sbjct: 121 IRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARF 180

Query: 192 DLNNDGKIDFHEFVKFMEVAL 212
           DLNNDGKIDFHEFVKFMEVAL
Sbjct: 181 DLNNDGKIDFHEFVKFMEVAL 198

[Close]

BLAST of MELO3C009858 vs. NCBI nr
Match: XP_038904514.1 (probable calcium-binding protein CML45 [Benincasa hispida])

HSP 1 Score: 234.2 bits (596), Expect = 1.0e-57
Identity = 138/192 (71.88%), Postives = 151/192 (78.65%), Query Frame = 0


Query: 22  SVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQS 81
           +VQL P+PLVFVD  S LPILF FA RIH+LI S+P  S  QS  SKEKA  QSNCSSQS
Sbjct: 9   NVQLIPTPLVFVDRTSILPILFTFADRIHDLI-SVPL-SFFQSP-SKEKASPQSNCSSQS 68

Query: 82  DDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGA--SDEIIRGMFEENE 141
           DD+ +IT Q+NC MSREEVRFVM+KLELF  +    +D DEEIG     E +  MFEENE
Sbjct: 69  DDQ-VITQQKNCLMSREEVRFVMDKLELFCSK----NDGDEEIGECFGGEELTAMFEENE 128

Query: 142 PSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKID 201
           PSLEELKQTFNVFDRN+DGFID  EL IVL LL+SN GIFIHDCK MIARFD NNDGKID
Sbjct: 129 PSLEELKQTFNVFDRNRDGFIDAEELHIVLGLLQSNNGIFIHDCKRMIARFDHNNDGKID 188

Query: 202 FHEFVKFMEVAL 212
           F EFVKFMEVAL
Sbjct: 189 FTEFVKFMEVAL 192

[Close]

BLAST of MELO3C009858 vs. NCBI nr
Match: XP_023528818.1 (probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 183.3 bits (464), Expect = 2.1e-42
Identity = 123/214 (57.48%), Postives = 140/214 (65.42%), Query Frame = 0


Query: 1   MFFRLEYHLITMQTQVT---NAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLP 60
           MFF+LEY L     Q T     + S      PLVF+D  S       FA  IH+LI  + 
Sbjct: 1   MFFKLEYLLTMHNPQPTINLQLIPSTTTTTPPLVFIDRAS------IFADTIHDLISLV- 60

Query: 61  YWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDD 120
            +S  QS   KEKA  QS  +SQS+ K      ++  MSREEV+ VMEKLEL+  EE DD
Sbjct: 61  -FSFFQSP-PKEKAAPQSR-ASQSEGK------EDWLMSREEVKSVMEKLELYCNEEEDD 120

Query: 121 DDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKG 180
           + D  E    DE +RGMFEENEPSLEELKQTFNVFDRN+DGFID  EL  VL LL SN  
Sbjct: 121 EID--ECFGRDE-VRGMFEENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNT 180

Query: 181 IFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 212
           IFIHDCK+MIARFD NND KIDF+EFVKFMEVAL
Sbjct: 181 IFIHDCKSMIARFDHNNDQKIDFNEFVKFMEVAL 195

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BLAST of MELO3C009858 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.1e-21
Identity = 68/179 (37.99%), Postives = 97/179 (54.19%), Query Frame = 0


Query: 32  FVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQ 91
           F  W+S+  I   F  R   L++        ++   +++ L++       DD        
Sbjct: 23  FCRWVSSTRI---FLSRFVPLLQHHQRVFDKKNNKDQQETLTKQEDDDDDDDDDDDDDDD 82

Query: 92  NCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNV 151
           +  +SREE   VM  L LF+    +DD   E+  A +  +  +FEE E SLEE+KQ F+V
Sbjct: 83  DIDISREEAEMVMRSLGLFY----NDDQLQEQYSAKE--VSSLFEEKEASLEEVKQAFDV 142

Query: 152 FDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVA 211
           FD NKDGFID  EL  VL++L   +G ++ +C  MI   D N DGKIDF+EFVKFME +
Sbjct: 143 FDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETS 192

[Close]

BLAST of MELO3C009858 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 6.5e-18
Identity = 69/215 (32.09%), Postives = 106/215 (49.30%), Query Frame = 0


Query: 7   YHLITMQTQVTNAVKSVQLDPSPLV---------FVDWISNLPILFNFAGRIHELIRSLP 66
           Y  ITM++ ++   +S  L   PL          F  W+S   + F+             
Sbjct: 7   YSFITMKSSLSKCKQSSSLS-FPLFGLINFFLIGFFRWVSFAQLFFS------------R 66

Query: 67  YWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDD 126
           +W ++Q Q    +  S+      S          +  + RE+V  VM+ L L      D 
Sbjct: 67  FWPLVQHQQCVSEKKSKDLEFQTSIKHEEYRDDDDDGLCREDVGMVMKSLGL----STDQ 126

Query: 127 DDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKG 186
           +++  +   S + +  +FEE EPSLEE+KQ F+VFD N+DGFID  +L  VL++L   +G
Sbjct: 127 ENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQG 186

Query: 187 IFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALC 213
             + +C+ MI  FD + DG+IDF+ FVKFME   C
Sbjct: 187 SNLENCRRMIRSFDGSKDGRIDFYGFVKFMENNFC 204

[Close]

BLAST of MELO3C009858 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.9e-10
Identity = 43/126 (34.13%), Postives = 74/126 (58.73%), Query Frame = 0


Query: 84  KIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLE 143
           K+++  + +   S EEV+ +++  E  +    ++ ++             + E+NE   +
Sbjct: 67  KVVVPNRADIKTSVEEVKAIIDDSEALYECLIEEGEE------------YLLEKNEMMGK 126

Query: 144 EL-KQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHE 203
           E+ K+ F +FD N+DGFIDE+EL  VLSLL  ++   + +C+ M+  +D N DGKIDF+E
Sbjct: 127 EIVKEAFRLFDENQDGFIDENELKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKIDFYE 179

Query: 204 FVKFME 209
           FVK +E
Sbjct: 187 FVKLIE 179

[Close]

BLAST of MELO3C009858 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.8e-10
Identity = 39/111 (35.14%), Postives = 67/111 (60.36%), Query Frame = 0


Query: 98  EEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKD 157
           +E+  ++EK+++      D   D +E G   E+ + + +E +   E++K+ FNVFD+N D
Sbjct: 40  KELTQMIEKIDV----NGDGCVDIDEFG---ELYKTIMDEEDEEEEDMKEAFNVFDQNGD 99

Query: 158 GFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 209
           GFI   EL  VLS L   +G  + DCK MI + D++ DG++++ EF + M+
Sbjct: 100 GFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

[Close]

BLAST of MELO3C009858 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.4e-09
Identity = 39/106 (36.79%), Postives = 64/106 (60.38%), Query Frame = 0


Query: 103 VMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDE 162
           ++EK++L      D   D EE G    + + + EE +   E++++ FNVFD+N+DGFI  
Sbjct: 45  MIEKIDL----NGDGYVDIEEFGG---LYQTIMEERDEE-EDMREAFNVFDQNRDGFITV 104

Query: 163 HELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 209
            EL  VL+ L   +G  + DCK MI++ D++ DG ++F EF + M+
Sbjct: 105 EELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEFKQMMK 142

[Close]

BLAST of MELO3C009858 vs. ExPASy TrEMBL
Match: A0A1S3B8I1 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 PE=4 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 7.0e-108
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 12  MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 71
           MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA
Sbjct: 1   MQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKA 60

Query: 72  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII 131
           LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII
Sbjct: 61  LSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEII 120

Query: 132 RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 191
           RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD
Sbjct: 121 RGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFD 180

Query: 192 LNNDGKIDFHEFVKFMEVALC 213
           LNNDGKIDFHEFVKFMEVALC
Sbjct: 181 LNNDGKIDFHEFVKFMEVALC 201

[Close]

BLAST of MELO3C009858 vs. ExPASy TrEMBL
Match: A0A0A0LHQ5 (Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G825010 PE=4 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 6.1e-88
Identity = 179/212 (84.43%), Postives = 192/212 (90.57%), Query Frame = 0


Query: 1   MFFRLEYHLITMQTQVTNAVKSVQLDPSPLVFVDWISNLPILFNFAGRIHELIRSLPYWS 60
           MFFRLEY+LITM+T++T+ +KSVQLDPSP VFVD IS LPILFNFA RI EL+RSLP W 
Sbjct: 1   MFFRLEYYLITMETRLTD-IKSVQLDPSPFVFVDRISILPILFNFADRIQELVRSLP-WP 60

Query: 61  ILQSQYSKEKALSQSNCSSQSDDKIIITTQQNC-SMSREEVRFVMEKLELFWREENDDDD 120
           ILQ  +SKEKALSQ NCSS+S D+IIITTQQNC SMSREEVRFVMEKLELFWREE D   
Sbjct: 61  ILQ-PHSKEKALSQCNCSSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGG 120

Query: 121 DDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIF 180
            D+ IG SDE+IRGMFEENEPSLEELKQTFNVFDRN+DGFIDEHELFIVLSLLESNKGIF
Sbjct: 121 GDKGIGESDEMIRGMFEENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIF 180

Query: 181 IHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 212
           IHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL
Sbjct: 181 IHDCKTMIARFDLNNDGKIDFHEFVKFMEVAL 209

[Close]

BLAST of MELO3C009858 vs. ExPASy TrEMBL
Match: A0A6J1J5Q6 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111481990 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 5.6e-41
Identity = 113/185 (61.08%), Postives = 130/185 (70.27%), Query Frame = 0


Query: 27  PSPLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKII 86
           P PLVF+D  S       FA  IH+L+ SL + S  QS   KEKA  QS  +SQS+ K  
Sbjct: 21  PPPLVFIDRAS------IFADTIHDLV-SLAF-SFFQSP-PKEKAAPQSR-ASQSEGK-- 80

Query: 87  ITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELK 146
               ++  MSREEV+ VMEKLEL+  EE DD+ D+       E +RGMFEENEPSLEELK
Sbjct: 81  ----EDWLMSREEVKSVMEKLELYCNEEGDDEIDE---CFGREEVRGMFEENEPSLEELK 140

Query: 147 QTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKF 206
           QTFNVFDRN+DGFID HEL  VL LL SN  IFIHDCK+MIARFD NND KIDF+EFVKF
Sbjct: 141 QTFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNNDEKIDFNEFVKF 186

Query: 207 MEVAL 212
           MEV+L
Sbjct: 201 MEVSL 186

[Close]

BLAST of MELO3C009858 vs. ExPASy TrEMBL
Match: A0A6J1F854 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111441729 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 5.6e-41
Identity = 118/196 (60.20%), Postives = 136/196 (69.39%), Query Frame = 0


Query: 22  SVQLDPS------PLVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQS 81
           ++QL PS      PLVF+D  S       FAG IH+L+ SL + S  QS   K+KA  QS
Sbjct: 9   NLQLIPSTTTTTPPLVFIDRAS------IFAGTIHDLV-SLAF-SFFQSP-PKDKAAPQS 68

Query: 82  NCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMF 141
             +SQS+ K      ++  MSREEV+ VMEKLEL+  EE DD+ D  E    DE +RGMF
Sbjct: 69  R-ASQSESK------EDWLMSREEVKSVMEKLELYCNEEEDDEID--ECFGRDE-VRGMF 128

Query: 142 EENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNND 201
           EENEPSLEELKQTFNVFDRN+DGFID  EL  VL LL SN  IFIHDCK+MIARFD NND
Sbjct: 129 EENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNND 185

Query: 202 GKIDFHEFVKFMEVAL 212
            KIDF+EFVKFMEVAL
Sbjct: 189 QKIDFNEFVKFMEVAL 185

[Close]

BLAST of MELO3C009858 vs. ExPASy TrEMBL
Match: A0A6J1EIK8 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111434428 PE=4 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 8.4e-37
Identity = 104/182 (57.14%), Postives = 124/182 (68.13%), Query Frame = 0


Query: 30  LVFVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKIIITT 89
           L+ VD IS   ILF+F  RIH+LI S P WS+  S   KEKA  Q N S +S+     T 
Sbjct: 16  LLLVDRISISSILFSFVDRIHDLI-SQP-WSLFHSP-PKEKASPQGN-SLESE----ATQ 75

Query: 90  QQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTF 149
            +N S+S+EEV+FVMEKLE F       D++ EE     E I  +F E+EPSLEELKQTF
Sbjct: 76  HKNSSISKEEVKFVMEKLEFFC-----SDEEIEESWLGGEEIGAIFYESEPSLEELKQTF 135

Query: 150 NVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEV 209
           NVFD+N+DG ID  EL  VL LL  N+G+FIHDC  MIARFD N DGKIDF+EFVKFME+
Sbjct: 136 NVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEFVKFMEI 184

Query: 210 AL 212
           AL
Sbjct: 196 AL 184

[Close]

BLAST of MELO3C009858 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.5e-22
Identity = 68/179 (37.99%), Postives = 97/179 (54.19%), Query Frame = 0


Query: 32  FVDWISNLPILFNFAGRIHELIRSLPYWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQ 91
           F  W+S+  I   F  R   L++        ++   +++ L++       DD        
Sbjct: 23  FCRWVSSTRI---FLSRFVPLLQHHQRVFDKKNNKDQQETLTKQEDDDDDDDDDDDDDDD 82

Query: 92  NCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNV 151
           +  +SREE   VM  L LF+    +DD   E+  A +  +  +FEE E SLEE+KQ F+V
Sbjct: 83  DIDISREEAEMVMRSLGLFY----NDDQLQEQYSAKE--VSSLFEEKEASLEEVKQAFDV 142

Query: 152 FDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVA 211
           FD NKDGFID  EL  VL++L   +G ++ +C  MI   D N DGKIDF+EFVKFME +
Sbjct: 143 FDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETS 192

[Close]

BLAST of MELO3C009858 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 92.4 bits (228), Expect = 4.6e-19
Identity = 69/215 (32.09%), Postives = 106/215 (49.30%), Query Frame = 0


Query: 7   YHLITMQTQVTNAVKSVQLDPSPLV---------FVDWISNLPILFNFAGRIHELIRSLP 66
           Y  ITM++ ++   +S  L   PL          F  W+S   + F+             
Sbjct: 7   YSFITMKSSLSKCKQSSSLS-FPLFGLINFFLIGFFRWVSFAQLFFS------------R 66

Query: 67  YWSILQSQYSKEKALSQSNCSSQSDDKIIITTQQNCSMSREEVRFVMEKLELFWREENDD 126
           +W ++Q Q    +  S+      S          +  + RE+V  VM+ L L      D 
Sbjct: 67  FWPLVQHQQCVSEKKSKDLEFQTSIKHEEYRDDDDDGLCREDVGMVMKSLGL----STDQ 126

Query: 127 DDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDEHELFIVLSLLESNKG 186
           +++  +   S + +  +FEE EPSLEE+KQ F+VFD N+DGFID  +L  VL++L   +G
Sbjct: 127 ENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQG 186

Query: 187 IFIHDCKTMIARFDLNNDGKIDFHEFVKFMEVALC 213
             + +C+ MI  FD + DG+IDF+ FVKFME   C
Sbjct: 187 SNLENCRRMIRSFDGSKDGRIDFYGFVKFMENNFC 204

[Close]

BLAST of MELO3C009858 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.1e-11
Identity = 43/126 (34.13%), Postives = 74/126 (58.73%), Query Frame = 0


Query: 84  KIIITTQQNCSMSREEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLE 143
           K+++  + +   S EEV+ +++  E  +    ++ ++             + E+NE   +
Sbjct: 67  KVVVPNRADIKTSVEEVKAIIDDSEALYECLIEEGEE------------YLLEKNEMMGK 126

Query: 144 EL-KQTFNVFDRNKDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHE 203
           E+ K+ F +FD N+DGFIDE+EL  VLSLL  ++   + +C+ M+  +D N DGKIDF+E
Sbjct: 127 EIVKEAFRLFDENQDGFIDENELKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKIDFYE 179

Query: 204 FVKFME 209
           FVK +E
Sbjct: 187 FVKLIE 179

[Close]

BLAST of MELO3C009858 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 66.6 bits (161), Expect = 2.7e-11
Identity = 39/111 (35.14%), Postives = 67/111 (60.36%), Query Frame = 0


Query: 98  EEVRFVMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKD 157
           +E+  ++EK+++      D   D +E G   E+ + + +E +   E++K+ FNVFD+N D
Sbjct: 40  KELTQMIEKIDV----NGDGCVDIDEFG---ELYKTIMDEEDEEEEDMKEAFNVFDQNGD 99

Query: 158 GFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 209
           GFI   EL  VLS L   +G  + DCK MI + D++ DG++++ EF + M+
Sbjct: 100 GFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

[Close]

BLAST of MELO3C009858 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 64.7 bits (156), Expect = 1.0e-10
Identity = 39/106 (36.79%), Postives = 64/106 (60.38%), Query Frame = 0


Query: 103 VMEKLELFWREENDDDDDDEEIGASDEIIRGMFEENEPSLEELKQTFNVFDRNKDGFIDE 162
           ++EK++L      D   D EE G    + + + EE +   E++++ FNVFD+N+DGFI  
Sbjct: 45  MIEKIDL----NGDGYVDIEEFGG---LYQTIMEERDEE-EDMREAFNVFDQNRDGFITV 104

Query: 163 HELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEFVKFME 209
            EL  VL+ L   +G  + DCK MI++ D++ DG ++F EF + M+
Sbjct: 105 EELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEFKQMMK 142

The following BLAST results are available for this feature:

BLAST of MELO3C009858 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008443616.1	1.4e-107	100.00	PREDICTED: probable calcium-binding protein CML30 [Cucumis melo]	[more]
KGN59556.1	1.3e-87	84.43	hypothetical protein Csa_001548 [Cucumis sativus]	[more]
XP_004147503.1	1.0e-81	84.08	probable calcium-binding protein CML45 [Cucumis sativus]	[more]
XP_038904514.1	1.0e-57	71.88	probable calcium-binding protein CML45 [Benincasa hispida]	[more]
XP_023528818.1	2.1e-42	57.48	probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MELO3C009858 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG5	2.1e-21	37.99	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q93Z27	6.5e-18	32.09	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9SN89	2.9e-10	34.13	Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ...	[more]
Q9LNE7	3.8e-10	35.14	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SRR7	1.4e-09	36.79	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]

BLAST of MELO3C009858 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B8I1	7.0e-108	100.00	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103487172 P...	[more]
A0A0A0LHQ5	6.1e-88	84.43	Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G82501...	[more]
A0A6J1J5Q6	5.6e-41	61.08	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114819...	[more]
A0A6J1F854	5.6e-41	60.20	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1EIK8	8.4e-37	57.14	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]

BLAST of MELO3C009858 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29000.1	1.5e-22	37.99	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	4.6e-19	32.09	Calcium-binding EF-hand family protein	[more]
AT3G47480.1	2.1e-11	34.13	Calcium-binding EF-hand family protein	[more]
AT1G05990.1	2.7e-11	35.14	EF hand calcium-binding protein family	[more]
AT3G07490.1	1.0e-10	36.79	ARF-GAP domain 11	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 182..210 e-value: 8.7E-4 score: 28.6 coord: 144..172 e-value: 2.2E-5 score: 33.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 143..208 e-value: 1.6E-9 score: 38.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 140..175 score: 13.760229
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 178..212 score: 11.054376
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 144..207 e-value: 2.26279E-15 score: 66.0321
None	No IPR available	GENE3D	1.10.238.10		coord: 69..210 e-value: 7.0E-19 score: 70.4
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 16..208
None	No IPR available	PANTHER	PTHR10891:SF745	CALCIUM-BINDING PROTEIN CML46-RELATED	coord: 16..208
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 191..203
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 153..165
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 136..207

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C009858.1	MELO3C009858.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding