MELO3C009837 (gene) Melon (DHL92) v4

Overview


Name	MELO3C009837
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	tRNA (Guanosine(18)-2'-O)-methyltransferase
Location	chr04: 27709577 .. 27712355 (-)
RNA-Seq Expression	MELO3C009837
Synteny	MELO3C009837

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTAGGGGAAGAGAGAAAAAAAATCTCTTCTTCTTAGTAACTGCTGGCGTGCTTTCTTCTTACCACTGTCTGAACCATTGAATCTCTTTCGACCAACATCCATGGCGTCCTGCCGAATCGTATCCCGTTCTTCTCTTACTCTGCTCCAAAGCTTCAGTCCCCGAAGAACTCAACGGGTTTTCCCTTCTGTTTTTCTCCCCTCTCTACACCTTCCCTCCTCTCCATTCTTCACTAACACACGTACCGTCTTCTCTCTTTCTCTCTATTGGACCCCAATCTTTACATTTCTTTTTTATTAATCTCTTCTTTACTTTACTTCTCTTTTGATTGCTATATAGTCTCAAGAAGCAACCCTAATGCAGTTTCTTCAAGATGTTTCGCTTCAATTTCCGCCGCTACATCTCCACTAGAACTAGCAGATATTGTGGCTGACGTAGAACCACCGGAGGTTCCTACACAAGACACGGTGGAGCACCTGCTCACGCATTCCGATGACGTTTCTAGGTTGATGAAGATGGAGCGGAGGCCGGTTGAGTTAGATGGAGCTGAATATCCACATAAAGGGCGGTGGTTTCCGTACCTGGATAAGTATAGCTTTGGAAGTTCTTGGTTGAATAGTGGTGAGATTTTGGAGGCCTTGGATTCTCTCATATTGGACACGAGGAAAGAGCGGTTCAAGAACGTGGTGCGGAATAGAAGCTACTCCGTTTGTTTGGTGGTGGAGGGATTGAGCGATTTTGGTAATGTCTCGGCGGCGTTTCGATCTGCTGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAACAACCATGGAGGTGAAATTGTTTTCGAATGCATTTGATTTTTCATTTCTTTCGAGAAAATTGTTTGATTCTGGAATTCGGAAGTTGGTTTGTTTTCAATATGATGGTGGAATTTCATTACTGTTCTTGTGCTTTTACTTTGTTGTTCAATGGCTCATTTGAATTCAATCAATTAGTCCGATGCTCTTGCATATGGTTAGAAGGTTAAACCGATCAAATACGTTTGATTTTGATTGTTAATTGTTAAATATGGATGACAATGTGACACAAATTGAGCTATGGAATTCCTGAAAATGTGCATCTCTTGTATATATTATGTCTTTATATGCATATAATCATTGAAACTTTATACCAAATATCGATTTTAATGTATTATTTTTCGTTGGACAACGCTTAGTTTCATCTGATTTTCCATGTGGAAGTATATGTTCGTATGTTTTTTCTTCCTTCATAGCTATCTTTATCTCGCCGTTTCATTACATTAAGTCATATTTGTCTTGGGCTTGAAACTTTTACCAGTGCTTGTATTCTCATGCTTTGATAAATTAGGGTGCCACATGAGTTCATTCAGCCTACTAAGAATGACAAAAGTTCCCATTGCTTACATTTTCTTTGTGAATCTTTTCTCATCTTTCTTCATCTGACATCATTGCAATTCAATTCAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCTACCTCAGAATGTTTTAAAATTCTAAAATCCCGGGGTTATAGAATAGTCTCTACACATCTAGGAGCGAATGCGGTATTGATTGATTTTGTACTTACCCTTCTTTCACATTGAAATTTTGTAAGCTTGTCCTGACAGCTGAATTGCGATTGTCCTGTTCCAGGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTGGGGAATGAAAGTAGGTAATTATCTTCTTGCTCCAGTAATATGGAGTATGATCAAGTTGATGAACGTTTTTGAATCACTGCAAGTTCGGAGATGTCGGTTAAATTTAGGAAATCATAACTGAATCCACGCTTGAAAATGCCAACAATTCTTTGAAATTGACAATTATCTCATGCTTCAGTTAGCAAATTTCTCCGCATGAATGAGTCGGAGTCTGGAGAAATTATACTTGTAGCTAGTTTAAAGTTTTAAACCTTTACAATATATATTGATTTCTGAGGTTAAGATTTCTTCCTTTTTTTAGGGGAATAAGTAATGAAGCCCTGGAGCTATCAGATTTGCATTGTAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTTTCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTATGCGATCGGACTTCTCGGCTGGTAAGTAACTATAAAAACCGTGTTTGAATACTTGGATCCATCATGCCATAAGGGCTCCTGTCTTTAACTCAATGTCGAAAATAAATATAAAATAAATATATACTGCTTCTATCATACAATTGATGGATGATGATGTTCTTAATGAAACCTTTCAGGGCTGTCATGGAGATCTTACATCAGAAGAAAGACAAATTCTACTCGCGGAGTTCTCTCTCAGGCATAGCAAGAGTGCAATCAGCATTGCGAACGAGTTGGCAAAGCGGAAGGGTTCCGCAATCTCTAGTACGGGGGTATTGCTGTAGAATGCCAAAAGTTTTGGCCGCGTGATGTGTAGGAAATGTTTATGAGCAGCAGAAACACAGGGAAACTTCATTCAAAGTTTTAGACTCAGGAAATCAGTTTATAAAGCCTTTTACATCAATTCATATCCCAAGAACTTTTACATCAATTCATATGCCTCTGTATAATGTTGTTGTAGTTCTCCATTTGACTTGGAGAAACGTTACCTTCTGCTTCATTTGGTAATTTTTTTTTTAATTTAAGCCTATAAATATACTGTGTTTTCATCTCCA

mRNA sequence

ATTAGGGGAAGAGAGAAAAAAAATCTCTTCTTCTTAGTAACTGCTGGCGTGCTTTCTTCTTACCACTGTCTGAACCATTGAATCTCTTTCGACCAACATCCATGGCGTCCTGCCGAATCGTATCCCGTTCTTCTCTTACTCTGCTCCAAAGCTTCAGTCCCCGAAGAACTCAACGGGTTTTCCCTTCTGTTTTTCTCCCCTCTCTACACCTTCCCTCCTCTCCATTCTTCACTAACACACTCTCAAGAAGCAACCCTAATGCAGTTTCTTCAAGATGTTTCGCTTCAATTTCCGCCGCTACATCTCCACTAGAACTAGCAGATATTGTGGCTGACGTAGAACCACCGGAGGTTCCTACACAAGACACGGTGGAGCACCTGCTCACGCATTCCGATGACGTTTCTAGGTTGATGAAGATGGAGCGGAGGCCGGTTGAGTTAGATGGAGCTGAATATCCACATAAAGGGCGGTGGTTTCCGTACCTGGATAAGTATAGCTTTGGAAGTTCTTGGTTGAATAGTGGTGAGATTTTGGAGGCCTTGGATTCTCTCATATTGGACACGAGGAAAGAGCGGTTCAAGAACGTGGTGCGGAATAGAAGCTACTCCGTTTGTTTGGTGGTGGAGGGATTGAGCGATTTTGGTAATGTCTCGGCGGCGTTTCGATCTGCTGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAACAACCATGGAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCTACCTCAGAATGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTGGGGAATGAAAGTAGGGGAATAAGTAATGAAGCCCTGGAGCTATCAGATTTGCATTGTAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTTTCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTATGCGATCGGACTTCTCGGCTGGGCTGTCATGGAGATCTTACATCAGAAGAAAGACAAATTCTACTCGCGGAGTTCTCTCTCAGGCATAGCAAGAGTGCAATCAGCATTGCGAACGAGTTGGCAAAGCGGAAGGGTTCCGCAATCTCTAGTACGGGGGTATTGCTGTAGAATGCCAAAAGTTTTGGCCGCGTGATGTGTAGGAAATGTTTATGAGCAGCAGAAACACAGGGAAACTTCATTCAAAGTTTTAGACTCAGGAAATCAGTTTATAAAGCCTTTTACATCAATTCATATCCCAAGAACTTTTACATCAATTCATATGCCTCTGTATAATGTTGTTGTAGTTCTCCATTTGACTTGGAGAAACGTTACCTTCTGCTTCATTTGGTAATTTTTTTTTTAATTTAAGCCTATAAATATACTGTGTTTTCATCTCCA

Coding sequence (CDS)

ATGGCGTCCTGCCGAATCGTATCCCGTTCTTCTCTTACTCTGCTCCAAAGCTTCAGTCCCCGAAGAACTCAACGGGTTTTCCCTTCTGTTTTTCTCCCCTCTCTACACCTTCCCTCCTCTCCATTCTTCACTAACACACTCTCAAGAAGCAACCCTAATGCAGTTTCTTCAAGATGTTTCGCTTCAATTTCCGCCGCTACATCTCCACTAGAACTAGCAGATATTGTGGCTGACGTAGAACCACCGGAGGTTCCTACACAAGACACGGTGGAGCACCTGCTCACGCATTCCGATGACGTTTCTAGGTTGATGAAGATGGAGCGGAGGCCGGTTGAGTTAGATGGAGCTGAATATCCACATAAAGGGCGGTGGTTTCCGTACCTGGATAAGTATAGCTTTGGAAGTTCTTGGTTGAATAGTGGTGAGATTTTGGAGGCCTTGGATTCTCTCATATTGGACACGAGGAAAGAGCGGTTCAAGAACGTGGTGCGGAATAGAAGCTACTCCGTTTGTTTGGTGGTGGAGGGATTGAGCGATTTTGGTAATGTCTCGGCGGCGTTTCGATCTGCTGATGCGCTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAACAACCATGGAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCTACCTCAGAATGTTTCTGTCTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTGGGGAATGAAAGTAGGGGAATAAGTAATGAAGCCCTGGAGCTATCAGATTTGCATTGTAGTATTCCTATGAATGGCATGGTAGATTCCTTCAACGTTTCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTATGCGATCGGACTTCTCGGCTGGGCTGTCATGGAGATCTTACATCAGAAGAAAGACAAATTCTACTCGCGGAGTTCTCTCTCAGGCATAGCAAGAGTGCAATCAGCATTGCGAACGAGTTGGCAAAGCGGAAGGGTTCCGCAATCTCTAGTACGGGGGTATTGCTGTAG

Protein sequence

MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPSSPFFTNTLSRSNPNAVSSRCFASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNHGGTERIVMLAWEQRNGWTLNFGTLPQNVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGSAISSTGVLL

Homology

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BLAST of MELO3C009837 vs. NCBI nr
Match: XP_008443364.1 (PREDICTED: tRNA (guanosine(18)-2'-O)-methyltransferase [Cucumis melo] >KAA0053795.1 tRNA (guanosine(18)-2'-O)-methyltransferase [Cucumis melo var. makuwa] >TYK25606.1 tRNA (guanosine(18)-2'-O)-methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 621.7 bits (1602), Expect = 3.9e-174
Identity = 330/369 (89.43%), Postives = 336/369 (91.06%), Query Frame = 0


Query: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPSSPFFTNTLSRSNPNAVSSRCF 60
           MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPSSPFFTNTLSRSNPNAVSSRCF
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPSSPFFTNTLSRSNPNAVSSRCF 60

Query: 61  ASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYPH 120
           ASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYPH
Sbjct: 61  ASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYPH 120

Query: 121 KGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSDF 180
           KGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSDF
Sbjct: 121 KGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSDF 180

Query: 181 GNVSAAFRSADALGFQSVHVVSCDASKRC--NNH---GGTERIVMLAWE---------QR 240
           GNVSAAFRSADALGFQSVHVVSCDASKR   N H   G  + + +  W          + 
Sbjct: 181 GNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTSECFKILKS 240

Query: 241 NGWTLNFGTLPQN-VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSF 300
            G+ +    L  N VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSF
Sbjct: 241 RGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSF 300

Query: 301 NVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGS 355
           NVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGS
Sbjct: 301 NVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGS 360

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BLAST of MELO3C009837 vs. NCBI nr
Match: XP_004136796.2 (uncharacterized protein LOC101218911 isoform X1 [Cucumis sativus])

HSP 1 Score: 575.5 bits (1482), Expect = 3.2e-160
Identity = 311/370 (84.05%), Postives = 325/370 (87.84%), Query Frame = 0


Query: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFTNTLSRSNPNAVSSRC 60
           MASCRIVSRSSL LLQS SPRRTQR+F SVFLPSLHLPS S FFTN  SRSNPNAVS+RC
Sbjct: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC 103

Query: 61  FASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYP 120
           FASISAATSPLE+ DIVADVEPPEV TQDTVEHLLTHS+DV+RLMKMERR VELDGAEYP
Sbjct: 104 FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 163

Query: 121 HKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSD 180
           HKGRWFPYLDKYS GSSWLNSGEILEALDSLILD+RKERFKNVVRNRSYSVCLVVEGL+D
Sbjct: 164 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 223

Query: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRC--NNH---GGTERIVMLAWE---------Q 240
           FGNVSAAFRSADALGFQSVHVVSCDASKR   N H   G  + + +  W          +
Sbjct: 224 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 283

Query: 241 RNGWTLNFGTLPQN-VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300
             G+ +    L  N VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS
Sbjct: 284 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 343

Query: 301 FNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 355
           FNVSVAAGILMHHAVC+RTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG
Sbjct: 344 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 403

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BLAST of MELO3C009837 vs. NCBI nr
Match: XP_011652219.1 (uncharacterized protein LOC101218911 isoform X2 [Cucumis sativus] >KAE8651207.1 hypothetical protein Csa_001530 [Cucumis sativus])

HSP 1 Score: 553.1 bits (1424), Expect = 1.7e-153
Identity = 303/370 (81.89%), Postives = 317/370 (85.68%), Query Frame = 0


Query: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFTNTLSRSNPNAVSSRC 60
           MASCRIVSRSSL LLQS SPRRTQR+F SVFLPSLHLPS S FFTN          S+RC
Sbjct: 44  MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRF--------STRC 103

Query: 61  FASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYP 120
           FASISAATSPLE+ DIVADVEPPEV TQDTVEHLLTHS+DV+RLMKMERR VELDGAEYP
Sbjct: 104 FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 163

Query: 121 HKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSD 180
           HKGRWFPYLDKYS GSSWLNSGEILEALDSLILD+RKERFKNVVRNRSYSVCLVVEGL+D
Sbjct: 164 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 223

Query: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRC--NNH---GGTERIVMLAWE---------Q 240
           FGNVSAAFRSADALGFQSVHVVSCDASKR   N H   G  + + +  W          +
Sbjct: 224 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 283

Query: 241 RNGWTLNFGTLPQN-VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300
             G+ +    L  N VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS
Sbjct: 284 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 343

Query: 301 FNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 355
           FNVSVAAGILMHHAVC+RTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG
Sbjct: 344 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 403

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BLAST of MELO3C009837 vs. NCBI nr
Match: XP_038905162.1 (uncharacterized protein LOC120091275 isoform X2 [Benincasa hispida])

HSP 1 Score: 543.5 bits (1399), Expect = 1.3e-150
Identity = 295/373 (79.09%), Postives = 314/373 (84.18%), Query Frame = 0


Query: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFF---TNTLSRSNPNAVS 60
           MASCRI +RSSL LL+S S RR Q +FPSV LPSL L S SPFF   TN LSRSNPNAVS
Sbjct: 1   MASCRIAARSSLILLESCSSRRPQSIFPSVSLPSLPLSSFSPFFTIVTNRLSRSNPNAVS 60

Query: 61  SRCFASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGA 120
           +RCFAS SAATS +E+AD V D EPPE+ TQDTVEHLLTHSDDV+RLMKMERRP ELDGA
Sbjct: 61  TRCFASFSAATSSIEVADAVGDREPPEIHTQDTVEHLLTHSDDVARLMKMERRPFELDGA 120

Query: 121 EYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEG 180
           EYPH+GRWFPYLDK+S GSSWLNSGEILEALD LILD+RKERFKNVVRNRSYSVCLVVEG
Sbjct: 121 EYPHRGRWFPYLDKFSCGSSWLNSGEILEALDPLILDSRKERFKNVVRNRSYSVCLVVEG 180

Query: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRC--NNH---GGTERIVMLAWE------- 240
           LSDFGNVSAAFRSADALGFQSVHVVSCDASKR   N H   G  + + +  W        
Sbjct: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFR 240

Query: 241 --QRNGWTLNFGTLPQN-VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGM 300
             +  G+ +    L  N VSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMNGM
Sbjct: 241 VLKSRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGM 300

Query: 301 VDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAK 355
           VDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAIS+ANE AK
Sbjct: 301 VDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISVANEFAK 360

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BLAST of MELO3C009837 vs. NCBI nr
Match: XP_038905163.1 (uncharacterized protein LOC120091275 isoform X3 [Benincasa hispida])

HSP 1 Score: 525.4 bits (1352), Expect = 3.8e-145
Identity = 286/370 (77.30%), Postives = 307/370 (82.97%), Query Frame = 0


Query: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFTNTLSRSNPNAVSSRC 60
           MASCRI +RSSL LL+S S RR Q +FPSV LPSL L S SPFFT   +R     +S+RC
Sbjct: 1   MASCRIAARSSLILLESCSSRRPQSIFPSVSLPSLPLSSFSPFFTIVTNR-----LSTRC 60

Query: 61  FASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYP 120
           FAS SAATS +E+AD V D EPPE+ TQDTVEHLLTHSDDV+RLMKMERRP ELDGAEYP
Sbjct: 61  FASFSAATSSIEVADAVGDREPPEIHTQDTVEHLLTHSDDVARLMKMERRPFELDGAEYP 120

Query: 121 HKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSD 180
           H+GRWFPYLDK+S GSSWLNSGEILEALD LILD+RKERFKNVVRNRSYSVCLVVEGLSD
Sbjct: 121 HRGRWFPYLDKFSCGSSWLNSGEILEALDPLILDSRKERFKNVVRNRSYSVCLVVEGLSD 180

Query: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRC--NNH---GGTERIVMLAWE---------Q 240
           FGNVSAAFRSADALGFQSVHVVSCDASKR   N H   G  + + +  W          +
Sbjct: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFRVLK 240

Query: 241 RNGWTLNFGTLPQN-VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300
             G+ +    L  N VSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMNGMVDS
Sbjct: 241 SRGYRIVSTHLGTNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNGMVDS 300

Query: 301 FNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 355
           FNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAIS+ANE AKRKG
Sbjct: 301 FNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISVANEFAKRKG 360

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BLAST of MELO3C009837 vs. ExPASy Swiss-Prot
Match: O67577 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=trmH PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.8e-15
Identity = 54/194 (27.84%), Postives = 100/194 (51.55%), Query Frame = 0


Query: 147 LDSLILDTRKERFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDAS 206
           ++ L+L+ R +R + V+  R   + +  + + +  N SA  R+ DA+G   ++    +  
Sbjct: 3   MEYLVLEKRLKRLREVLEKRQKDLIVFADNVKNEHNFSAIVRTCDAVGVLYLYYYHAEGK 62

Query: 207 KRCNNHG---GTERIV-----------MLAWEQRNGWTLNFGTLPQNVSVYDMDWSCPTA 266
           K   N G   G+ + V           +L ++ R    +      ++V+  ++D++ PT 
Sbjct: 63  KAKINEGITQGSHKWVFIEKVDNPVQKLLEFKNRGFQIVATWLSKESVNFREVDYTKPTV 122

Query: 267 IVVGNESRGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR-LGCHGD 326
           +VVGNE +G+S E +E++D    IPM GM  S NVSVA GI+++ A   R  + +     
Sbjct: 123 LVVGNELQGVSPEIVEIADKKIVIPMYGMAQSLNVSVATGIILYEAQRQREEKGMYSRPS 182

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BLAST of MELO3C009837 vs. ExPASy Swiss-Prot
Match: Q72GI1 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=trmH PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.6e-13
Identity = 50/159 (31.45%), Postives = 83/159 (52.20%), Query Frame = 0


Query: 153 DTRKERFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNH 212
           + R+ R + V+R R   + +++E +    N+SA  R+ DA+G    H V+        N 
Sbjct: 6   EARRRRIEEVLRRRQPDLTVLLENVHKPHNLSAILRTCDAVGVLEAHAVNPTGGVPTFNE 65

Query: 213 --GGTERIVML----------AWEQRNGWTLNFGTLPQNVSVY-DMDWSCPTAIVVGNES 272
             GG+ + V L           + +  G+T+    L ++   + ++D++ PTA++ G E 
Sbjct: 66  TSGGSHKWVYLRVHPDLHEAFRFLKERGFTVYATALREDARDFREVDYTKPTAVLFGAEK 125

Query: 273 RGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHA 299
            G+S EAL L+D    IPM GMV S NVSVAA +++  A
Sbjct: 126 WGVSEEALALADGAIKIPMLGMVQSLNVSVAAAVILFEA 164

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BLAST of MELO3C009837 vs. ExPASy Swiss-Prot
Match: Q5SM16 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=trmH PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.6e-13
Identity = 50/159 (31.45%), Postives = 83/159 (52.20%), Query Frame = 0


Query: 153 DTRKERFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNH 212
           + R+ R + V+R R   + +++E +    N+SA  R+ DA+G    H V+        N 
Sbjct: 6   EARRRRIEEVLRRRQPDLTVLLENVHKPHNLSAILRTCDAVGVLEAHAVNPTGGVPTFNE 65

Query: 213 --GGTERIVML----------AWEQRNGWTLNFGTLPQNVSVY-DMDWSCPTAIVVGNES 272
             GG+ + V L           + +  G+T+    L ++   + ++D++ PTA++ G E 
Sbjct: 66  TSGGSHKWVYLRVHPDLHEAFRFLKERGFTVYATALREDARDFREVDYTKPTAVLFGAEK 125

Query: 273 RGISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHA 299
            G+S EAL L+D    IPM GMV S NVSVAA +++  A
Sbjct: 126 WGVSEEALALADGAIKIPMLGMVQSLNVSVAAAVILFEA 164

[Close]

BLAST of MELO3C009837 vs. ExPASy Swiss-Prot
Match: P0AGJ3 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334 GN=trmH PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 6.8e-12
Identity = 65/207 (31.40%), Postives = 96/207 (46.38%), Query Frame = 0


Query: 154 TRKERFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVV----------SC 213
           TR  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V          S 
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASA 63

Query: 214 DASKRCNNHGGTERIV--MLAWEQRNGWTLNFGTLPQN-VSVYDMDWSCPTAIVVGNESR 273
            A         T R +   +A  +  G  +    L  N V   ++D++ PT I++G E  
Sbjct: 64  AAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKT 123

Query: 274 GISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSEER 333
           GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   E+
Sbjct: 124 GITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEAEQ 183

Query: 334 QILLAEFSLRHSKSAISIANELAKRKG 345
           Q LL E           +  ++AKRKG
Sbjct: 184 QRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of MELO3C009837 vs. ExPASy Swiss-Prot
Match: P0AGJ2 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=trmH PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 6.8e-12
Identity = 65/207 (31.40%), Postives = 96/207 (46.38%), Query Frame = 0


Query: 154 TRKERFKNVVRNRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVV----------SC 213
           TR  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V          S 
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVWPGSRMRTMASA 63

Query: 214 DASKRCNNHGGTERIV--MLAWEQRNGWTLNFGTLPQN-VSVYDMDWSCPTAIVVGNESR 273
            A         T R +   +A  +  G  +    L  N V   ++D++ PT I++G E  
Sbjct: 64  AAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQEKT 123

Query: 274 GISNEALELSDLHCSIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSEER 333
           GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   E+
Sbjct: 124 GITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEAEQ 183

Query: 334 QILLAEFSLRHSKSAISIANELAKRKG 345
           Q LL E           +  ++AKRKG
Sbjct: 184 QRLLFE-------GGYPVLAKVAKRKG 203

[Close]

BLAST of MELO3C009837 vs. ExPASy TrEMBL
Match: A0A5A7UJ80 (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007650 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 1.9e-174
Identity = 330/369 (89.43%), Postives = 336/369 (91.06%), Query Frame = 0


Query: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPSSPFFTNTLSRSNPNAVSSRCF 60
           MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPSSPFFTNTLSRSNPNAVSSRCF
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPSSPFFTNTLSRSNPNAVSSRCF 60

Query: 61  ASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYPH 120
           ASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYPH
Sbjct: 61  ASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYPH 120

Query: 121 KGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSDF 180
           KGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSDF
Sbjct: 121 KGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSDF 180

Query: 181 GNVSAAFRSADALGFQSVHVVSCDASKRC--NNH---GGTERIVMLAWE---------QR 240
           GNVSAAFRSADALGFQSVHVVSCDASKR   N H   G  + + +  W          + 
Sbjct: 181 GNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTSECFKILKS 240

Query: 241 NGWTLNFGTLPQN-VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSF 300
            G+ +    L  N VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSF
Sbjct: 241 RGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSF 300

Query: 301 NVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGS 355
           NVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGS
Sbjct: 301 NVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGS 360

[Close]

BLAST of MELO3C009837 vs. ExPASy TrEMBL
Match: A0A1S3B8M3 (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486969 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 1.9e-174
Identity = 330/369 (89.43%), Postives = 336/369 (91.06%), Query Frame = 0


Query: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPSSPFFTNTLSRSNPNAVSSRCF 60
           MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPSSPFFTNTLSRSNPNAVSSRCF
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPSSPFFTNTLSRSNPNAVSSRCF 60

Query: 61  ASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYPH 120
           ASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYPH
Sbjct: 61  ASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYPH 120

Query: 121 KGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSDF 180
           KGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSDF
Sbjct: 121 KGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSDF 180

Query: 181 GNVSAAFRSADALGFQSVHVVSCDASKRC--NNH---GGTERIVMLAWE---------QR 240
           GNVSAAFRSADALGFQSVHVVSCDASKR   N H   G  + + +  W          + 
Sbjct: 181 GNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTSECFKILKS 240

Query: 241 NGWTLNFGTLPQN-VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSF 300
            G+ +    L  N VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSF
Sbjct: 241 RGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDSF 300

Query: 301 NVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGS 355
           NVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGS
Sbjct: 301 NVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKGS 360

[Close]

BLAST of MELO3C009837 vs. ExPASy TrEMBL
Match: A0A0A0LC15 (SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824750 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.6e-160
Identity = 311/370 (84.05%), Postives = 325/370 (87.84%), Query Frame = 0


Query: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFTNTLSRSNPNAVSSRC 60
           MASCRIVSRSSL LLQS SPRRTQR+F SVFLPSLHLPS S FFTN  SRSNPNAVS+RC
Sbjct: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFTNRFSRSNPNAVSTRC 60

Query: 61  FASISAATSPLELADIVADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDGAEYP 120
           FASISAATSPLE+ DIVADVEPPEV TQDTVEHLLTHS+DV+RLMKMERR VELDGAEYP
Sbjct: 61  FASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGAEYP 120

Query: 121 HKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVEGLSD 180
           HKGRWFPYLDKYS GSSWLNSGEILEALDSLILD+RKERFKNVVRNRSYSVCLVVEGL+D
Sbjct: 121 HKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEGLTD 180

Query: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRC--NNH---GGTERIVMLAWE---------Q 240
           FGNVSAAFRSADALGFQSVHVVSCDASKR   N H   G  + + +  W          +
Sbjct: 181 FGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFKILK 240

Query: 241 RNGWTLNFGTLPQN-VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300
             G+ +    L  N VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS
Sbjct: 241 SRGYRIVSTHLGANAVSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNGMVDS 300

Query: 301 FNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 355
           FNVSVAAGILMHHAVC+RTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG
Sbjct: 301 FNVSVAAGILMHHAVCNRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELAKRKG 360

[Close]

BLAST of MELO3C009837 vs. ExPASy TrEMBL
Match: A0A6J1F0V5 (uncharacterized protein LOC111441136 OS=Cucurbita moschata OX=3662 GN=LOC111441136 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.5e-134
Identity = 270/374 (72.19%), Postives = 299/374 (79.95%), Query Frame = 0


Query: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFT---NTLSRSNPNAVS 60
           MASCRI  R SL LL S SPR TQR+ PSV   S  L S SPFFT   N  SR+NP AVS
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 61  SRCFASISAATSPLELADIV-ADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDG 120
            RCF+S SAA S +E+ D V +D+EPPE+ TQDTVEHLLTHSDDV+RLMKMERRP+ LDG
Sbjct: 61  IRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 AEYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVE 180
            EYP +GRWFPYLDK+S  SSWLNSGE+L+ALD LILD+RKERFKNVVR+RSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRC--NNH---GGTERIVMLAWE------ 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKR   N H   G  + + +  W       
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 ---QRNGWTLNFGTLPQN-VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNG 300
              +  G+ +    L  N VSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHCSIPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCSIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELA 355
           MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTS ERQILLAEFSLRH+K+AIS+A+E A
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

[Close]

BLAST of MELO3C009837 vs. ExPASy TrEMBL
Match: A0A6J1HYK4 (uncharacterized protein LOC111468788 OS=Cucurbita maxima OX=3661 GN=LOC111468788 PE=3 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.5e-134
Identity = 267/369 (72.36%), Postives = 298/369 (80.76%), Query Frame = 0


Query: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFT---NTLSRSNPNAVS 60
           MASCRI  R SL LL S S R TQ + PSV  PS  L S SPFFT   N  SR+NP AV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60

Query: 61  SRCFASISAATSPLELADIV-ADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDG 120
           +RCF+S SAA S +E+ D V +D+EPPE+ TQDTVEHLLTHSDDV+RLMKMERRP+ LDG
Sbjct: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 AEYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVE 180
           AEYPH+GRWFPYLDK+S  +SWLNSGE+L+ALD LILD+RKERFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRC--NNH---GGTERIVMLAWE------ 240
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKR   N H   G  + + +  W       
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECF 240

Query: 241 ---QRNGWTLNFGTLPQN-VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIPMNG 300
              +  G+ +    L  N VSVYDMDWSCPTAIVVGNESRGIS+EALELSDLHC+IPMNG
Sbjct: 241 RILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNG 300

Query: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIANELA 350
           MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTS ERQILLAEFSLRH+K+AIS+A+E A
Sbjct: 301 MVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYA 360

[Close]

BLAST of MELO3C009837 vs. TAIR 10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 302.8 bits (774), Expect = 3.7e-82
Identity = 190/366 (51.91%), Postives = 240/366 (65.57%), Query Frame = 0


Query: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPSSPFFT----NTLSRSNPNA-- 60
           M SC+ + R+S++             FP V LP+ +  S  F +    + LS  +PN+  
Sbjct: 1   MNSCKSLIRASIS------------SFPFVPLPNPNF-SITFISVRAFSPLSVLHPNSSC 60

Query: 61  -VSSRCFASISAATSPLELADIVADVEPPEVPTQDTVEHLL-THSDDVSRLMKMERRPVE 120
            V++R     + A SP  L +     E P    +DTV+ LL T+  + S LMKMERR   
Sbjct: 61  IVTARRTFHGAVALSPESLTE-----ESP----KDTVKGLLTTNRGEASSLMKMERR-CS 120

Query: 121 LDGAEYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCL 180
           L   E   +G WFPY D++  G   L+S E+LEA+   +++ R +RF+ VV NRSYSVCL
Sbjct: 121 LSNGEGDCRGSWFPYEDRFRCGEVHLSSREVLEAVSPHMMEERTDRFRRVVENRSYSVCL 180

Query: 181 VVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCNNH-----GGTERIVMLAWE--- 240
           VVEGLSDFGN+SAAFRSADALG QSVHVVSCD+SKR N +     G  + + +  W+   
Sbjct: 181 VVEGLSDFGNISAAFRSADALGIQSVHVVSCDSSKRYNGNRHVSMGAEKWLDIEFWDTPK 240

Query: 241 ------QRNGWTLNFGTLPQN-VSVYDMDWSCPTAIVVGNESRGISNEALELSDLHCSIP 300
                 +  G+ +    L  + VS+YDMDWSCPTAIVVGNE RGIS+EALELSDL CSIP
Sbjct: 241 ECFKVLKSRGYRIATTHLGMDTVSIYDMDWSCPTAIVVGNEGRGISDEALELSDLRCSIP 300

Query: 301 MNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSEERQILLAEFSLRHSKSAISIAN 344
           MNGMVDSFNVSVAAGILMHHAV DRT+RLG HGDL+  E++IL+AEFSLRHS+S+I IA 
Sbjct: 301 MNGMVDSFNVSVAAGILMHHAVSDRTTRLGSHGDLSEAEKEILMAEFSLRHSRSSICIAY 343

[Close]

BLAST of MELO3C009837 vs. TAIR 10
Match: AT2G19870.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 43.5 bits (101), Expect = 4.1e-04
Identity = 40/173 (23.12%), Postives = 76/173 (43.93%), Query Frame = 0


Query: 168 YSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKR----CNNHGGTERIVMLAW 227
           YS+ + ++ ++D  N+ A  RSA   G   V V + +++           G+  ++ L +
Sbjct: 416 YSLWVALDEVTDPQNLGAIIRSAYFFGATGVVVCAKNSAPLSAVVSKASAGSLEVMELRY 475

Query: 228 ----------EQRNGWTLNFGTL-PQNVSVYDMDWSCPTAIVVGNESRG----ISNEALE 287
                        NGW +  G++ P+ V++ ++    PT +V+GNE  G    +     +
Sbjct: 476 CKNMMQFLEASAENGWRVVGGSVSPKAVALNEVLPGSPTILVLGNEGTGLRPLVERSCTD 535

Query: 288 LSDLHCSIPMNG-----------------MVDSFNVSVAAGILMHHAVCDRTS 305
           L  +  ++P                     V+S NVSVAAG+ +HH + ++ S
Sbjct: 536 LVRISGNMPNEVAVTESDDAEGEGFRSFLAVESLNVSVAAGLFLHHLIGNKAS 588

The following BLAST results are available for this feature:

BLAST of MELO3C009837 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008443364.1	3.9e-174	89.43	PREDICTED: tRNA (guanosine(18)-2'-O)-methyltransferase [Cucumis melo] >KAA005379...	[more]
XP_004136796.2	3.2e-160	84.05	uncharacterized protein LOC101218911 isoform X1 [Cucumis sativus]	[more]
XP_011652219.1	1.7e-153	81.89	uncharacterized protein LOC101218911 isoform X2 [Cucumis sativus] >KAE8651207.1 ...	[more]
XP_038905162.1	1.3e-150	79.09	uncharacterized protein LOC120091275 isoform X2 [Benincasa hispida]	[more]
XP_038905163.1	3.8e-145	77.30	uncharacterized protein LOC120091275 isoform X3 [Benincasa hispida]	[more]

BLAST of MELO3C009837 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O67577	3.8e-15	27.84	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=...	[more]
Q72GI1	3.6e-13	31.45	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC...	[more]
Q5SM16	3.6e-13	31.45	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC...	[more]
P0AGJ3	6.8e-12	31.40	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334...	[more]
P0AGJ2	6.8e-12	31.40	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=...	[more]

BLAST of MELO3C009837 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UJ80	1.9e-174	89.43	tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3B8M3	1.9e-174	89.43	tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486...	[more]
A0A0A0LC15	1.6e-160	84.05	SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824...	[more]
A0A6J1F0V5	1.5e-134	72.19	uncharacterized protein LOC111441136 OS=Cucurbita moschata OX=3662 GN=LOC1114411...	[more]
A0A6J1HYK4	2.5e-134	72.36	uncharacterized protein LOC111468788 OS=Cucurbita maxima OX=3661 GN=LOC111468788...	[more]

BLAST of MELO3C009837 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15390.1	3.7e-82	51.91	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT2G19870.1	4.1e-04	23.12	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 170..296 e-value: 1.1E-22 score: 80.8
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 146..337 e-value: 2.2E-42 score: 146.6
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	PANTHER	PTHR43453	RRNA METHYLASE-LIKE	coord: 1..347
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	CDD	cd18092	SpoU-like_TrmH	coord: 155..302 e-value: 2.88615E-51 score: 166.103
None	No IPR available	PANTHER	PTHR43453:SF1	RRNA METHYLASE-LIKE PROTEIN	coord: 1..347
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 151..307

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C009837.1	MELO3C009837.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006396	RNA processing
biological_process	GO:0030488	tRNA methylation
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008173	RNA methyltransferase activity