MELO3C007688 (gene) Melon (DHL92) v4

Overview


Name	MELO3C007688
Type	gene
Organism	Cucumis melo cv. DHL92 (Melon (DHL92) v4)
Description	tRNA-dihydrouridine synthase
Location	chr08: 4647889 .. 4652337 (+)
RNA-Seq Expression	MELO3C007688
Synteny	MELO3C007688

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATTTAACCAATGGTTAACTGTCGGGCATGAGTAGCGATCGAGTCCAAATTTCCTAAACCTAAGTTAGAGTCTCGTCCTTAGTTTTCCGGCCCAAAGTTCACAAGAAGAGAAGACGAAGAAATTGTTGCCGATTTTCTAGTCCGACCGTCTCCGTTTAAGATGGATTACGAGAACAAACTCGTCCTAGCTCCCATGGTTCGCGTTGTAAGTTACACCAAATTATAATATTTGTCGCTTGAGTTGCTCTCATTACTTGATCGAGTTTACCTTCTAATCTTAGTTTTTGTAAAAAAAATTGCCGCTCGATTGTCTACATCTAGATGCCAATCATTTCGAATAGTTTGAAGCTAGATTAGTAAATCACTCAATCGTAGTCTTTTCTTGCAAAATACCCGCCAGATTGTTACCATTTAGGTTGCTGATCAAAGTTATGGAGAATTGTGAATATGATGAGATTATGTGTTTCGAATTACAAAACATGTTTTTTTCATGGTGTTTTCTGTTATTGGCCCTTCATGTTTTCAGGGAACGTTACCGTTTAGATTATTAGCTTCACAATACGGTGCAGACATTACCTATGGCGAGGAAATTATTGACCATAAGATGATCAAATGCGAGCGTAGATTAAATGGTAGTGGTACTTTTCTCCTCTGTATTTTTTAAAAATTGAAGTTATTGGTTTTTAATTGTACTCTCACACCAAATGCAATATTTATCAAATAGCAGTGTTACTCTATTCATACGAAGTCTGTTTCACCCAGAAAAATGATATATTTGAATTCACCTGTAGAGGGAGATTTTCGCCGTCGTATGCATATTGGCAATAAGTTACTGTGTTCATTTATTGATAATTATGGGTCTGTTTGTTTAATGTTATCCTCTATCCATTTTTTTCAATGTTGTATTTCAAGTCATAACAATTTGTCGGAATTTTTATTTTTCGTCTATAAATCTGGCTTCAATTGGAAGTTAGAAGGAAAGATGTGATTAGTTAGCGGCTTAAGAGATGAAGGTTGCATTAAGCATATTTTCTTCCCTTTTCTTTACCAATGATGGTCTAGTGAACTTCCTGTACTTCTCTTCATCCAGAGCATGTAGGTTCAACTGATTTTGTTGAGAAGGGGACAAACAATGTAGTTTTCAGGACCTGCAGCCTAGAAAGAAAACATGTAGTTTTTCAAATGGGTACTTCTAATGCTGCGAGGGCTCTAAGTGCTGCTCAGTTAGTGTAAGTTTGTTACTTCCATTTTTCCCTTCATTACGCAATTGGCGGTCTTATGAATTTTGGTGGTGGTTATCCTCACTTCATACTTTATATACATACACGTGTCATATGTGAGTGGTGTGCCAGCTTATGCACACCTTGCTCTGTACTATATTCTTACTATTATTATTTATCCTTCAATTTATTGTAATGAGCTCTGTTATGGATTGAACTTAATCTTAGTGGCATTTTAAAAAGAATATATTTGTTTATTTACTTGTTCGATTAATGTAATGATGTGATTTATCTCTTAGTAGCCTCTTTTGTGGTCACTTTTTAATGGCAATTTTTTTCGCATTATTTATTTTAATGTGATCATCTTGATAACCATTCGGGGTTCATTTGGCTTCGTTGATTATGTCTCATACTCTTTCATTGCGGTTCTTCTGTTCAATTTCTTGCAACATGAAATCTATAATTGATTAGGCGTACATTGACGATCAGCAACAGTATGGAGTCAAGTAGTTGCTATGATGACAGATAAAAGGCTTATTTTCTTGATACCCTTAAATGTTCACTAATTAAGTGAAAGATTGCTGTGAGCAGTTGCACCATATCACTAGGCTCTCTACGGATTACTTCTGTGTTAGGAGATAATTGGGTACTTAATTGGTGTAGAATGCTTCTTTTTCTAATGAAACCATATTTGTATAATTGTTTGAAAATTTTATTTTTGTAGGTGTCGAGACGTTGCTGCCATAGATATCAATATGGGTTGCCCTAAGTCATTTTCTATCAGTGGGGGCATGGGTGCGGCACTGTTGAATAAACCAGACCTTATTCATGATGTATGCAATTCATCTCCTTACGGATTGATATAATTTCTGATTTATTCTCCCTCTTTTGTATTTCTGGCAATATTACTTTCCTACTCACCTTTTATTTACTTGTTTTTTCAGATTTTGACAACATTGAGGAGAAACTTGGACGTTCTAATTACCTGTAAGATTCGACTTTTAAATTCACCTCAGGATACAGTGGAGTTAGCTAGACGAATAGAAACTACTGGTGTTTCAGCTCTTGGTGTTCATGGAAGGTGATCGTCAAGTTTTTGCTGTTTCCTTCTATTTAAGATAGTTACGATTTATGCCAGAAGAATTTAGTTGTAGAATAAGGTCAGTGGGTTGTCCCTAAGCAAGTTGGTCTGGACACTCATGTCTATTAAAGAAACGAAATTTAGTTTTTTTCCTTCTCCGCCCAAGGCAGAGGGTTGAGGAAACCCCCACCCACAAAACTAATAAAGGAGAGCCCGCCAATTGTTAATAGTCAATAGAAGAATATCATTACAACAGAGGACATTCATTTGCTACAAAACATTTCGATATACTTTTGTGCTTTAAGAAGGGCAGTTACTTAGATGTCTCTTGCATTTGAGGCCTAAAAATTTGTAATTCATTCTAGTCCTAAAGATAGTAGGATTGAGAGATTGCTAGGAAGTAAGCAGAATAATGAATATATAGTACCAAGCATGCTCAGAGGGAAAAATTTAAAACTATGGTGAATATTATGCTTTGTTGTTTCTTTCATTTTTTTCCTCAACAATAGTGGTTCTTTTTATCTCTCTCTCTCTCTCACACACACACGCACAAAAGAATTTGCTGCACTGGGAGAGTCCACTTGTATAAAAGGTTGCTGGGTATGGGTATTTTATACCATTTTAGGTTGGCATTGAATTGTGTAATTTGTATTATAATAATGAAAAAAGGACAGAGAACTGAGCTGCGTTGCCACAGCTGAAAACAAAAGAAAGTGAAAGAATAAACTAGATTTGATCAGACCTTTGAGTTCCAAATGATACTTTTGTTAGCTTTGCATGAATGCTTTGTTTTCATTCTATTTTAATTGGTTTTTGTAGTCATGCTCGATAACTTGCAGAAGAGTTGCAGACAGACCAAGAGATCCAGCTAAATGGAGTGAGATTGCAGATGTTGTAGCAGCATTGACTATTCCTGTTATTGCAAATGGTGATATCTTTGAGTATGATGATTTTCAACGGATCAAAGTTCATACAGGTATGTTATTACAGAAGGCACTGTTTTTGTTTTGTGTGTGTGTGTGCGGGAGGAGGGGCGGTACTACTAATTGGAACTCTTTAAATCTGATTGTACCTCATTCACTAAGAGGCTGCTTTTGCCTGGCTTGATCTCTTTCATTTCTTTTACTGGTCTTCTCGTAAACAAAATAGACAACAGCCGTTTTTACTAGATACACTCATTTATCCTTTGATATACCAACCAGTGAAGCAATATGGCGATAGCGAGTTAGTTTTGAACTAGAGGTAGAGGTCCAAAATGTCTTATAAGTTACCCAGCTACCGTGATGTACTGTCAGAAAATAGTCTTGTGAGGATTATTAAGGAATGCACAAGCCTCAAGCACGATATTTCTGACCATTGATCGGGAAAATCCAGTTATCCAATAATTGCCGAATGTGGAAGAAACAATTTGGAAAAAATTTCAGTTCTTAACCTACAAGGCTGTGAAATAGTTTCAATGCTTTTTGAATTAGCAAATAAACTAATTATGGTGTTGAAAATCCATATGGACGTCTTTTTGAATCATCAACCTTGGTGTTACTGTTACTTCAACTTGTTAACCATGTTGAATGGTGTAAACACATGGTCTTGATCTGTGCGTTCCTTTGGTGGAAGAGAGTGTGTGGTTAAATTTAGTTGACATGAATGAACTATAGTAATGTTGTAATTCATCACGGATGAAGCTGCTTGGGCCGACAGAGATGCTATTTCAAGCAGTTTAGGCTTTTACTTGTCTGTCCATTCTCTCTTAAGGTGCTTCTTCACTGATGGTTGCTCGAGGAGCTCTTTGGAATGCTTCAATCTTCTCTCCAGAAGGCAAATTACCATGGGAAGATGTGAAAAGAGAATATGTTAGGAAGGTGAGATACTTATCATGCACACAGTCAATTCAGTCAATTAATTAATTAATGAGAGTGAATGCTTTAGAGTTTTATAGTTCCCTCGCTAGATTTTACGTATGTAGATACACACACACAAACAGAACTGAAGTGATTAAATTCTTGTGTTGGACTCAGAGCATCTTGTGGGATAATGATATCAAAAGTACAAAGTATACGCTAAAAGAAATGATAATGCATCATTCTTGTCTTGAACTGCCAGAAGGAAAGGCCGTGATAAAATCTGAATCCCTTGCAGACCTAGCGTAA

mRNA sequence

AAATTTAACCAATGGTTAACTGTCGGGCATGAGTAGCGATCGAGTCCAAATTTCCTAAACCTAAGTTAGAGTCTCGTCCTTAGTTTTCCGGCCCAAAGTTCACAAGAAGAGAAGACGAAGAAATTGTTGCCGATTTTCTAGTCCGACCGTCTCCGTTTAAGATGGATTACGAGAACAAACTCGTCCTAGCTCCCATGGTTCGCGTTGGAACGTTACCGTTTAGATTATTAGCTTCACAATACGGTGCAGACATTACCTATGGCGAGGAAATTATTGACCATAAGATGATCAAATGCGAGCGTAGATTAAATGAGCATGTAGGTTCAACTGATTTTGTTGAGAAGGGGACAAACAATGTAGTTTTCAGGACCTGCAGCCTAGAAAGAAAACATGTAGTTTTTCAAATGGGTACTTCTAATGCTGCGAGGGCTCTAAGTGCTGCTCAGTTAGTGTGTCGAGACGTTGCTGCCATAGATATCAATATGGGTTGCCCTAAGTCATTTTCTATCAGTGGGGGCATGGGTGCGGCACTGTTGAATAAACCAGACCTTATTCATGATATTTTGACAACATTGAGGAGAAACTTGGACGTTCTAATTACCTGTAAGATTCGACTTTTAAATTCACCTCAGGATACAGTGGAGTTAGCTAGACGAATAGAAACTACTGGTGTTTCAGCTCTTGGTGTTCATGGAAGAAGAGTTGCAGACAGACCAAGAGATCCAGCTAAATGGAGTGAGATTGCAGATGTTGTAGCAGCATTGACTATTCCTGTTATTGCAAATGGTGATATCTTTGAGTATGATGATTTTCAACGGATCAAAGTTCATACAGGTGCTTCTTCACTGATGGTTGCTCGAGGAGCTCTTTGGAATGCTTCAATCTTCTCTCCAGAAGGCAAATTACCATGGGAAGATGTGAAAAGAGAATATGTTAGGAAGAGCATCTTGTGGGATAATGATATCAAAAGTACAAAGTATACGCTAAAAGAAATGATAATGCATCATTCTTGTCTTGAACTGCCAGAAGGAAAGGCCGTGATAAAATCTGAATCCCTTGCAGACCTAGCGTAA

Coding sequence (CDS)

ATGGATTACGAGAACAAACTCGTCCTAGCTCCCATGGTTCGCGTTGGAACGTTACCGTTTAGATTATTAGCTTCACAATACGGTGCAGACATTACCTATGGCGAGGAAATTATTGACCATAAGATGATCAAATGCGAGCGTAGATTAAATGAGCATGTAGGTTCAACTGATTTTGTTGAGAAGGGGACAAACAATGTAGTTTTCAGGACCTGCAGCCTAGAAAGAAAACATGTAGTTTTTCAAATGGGTACTTCTAATGCTGCGAGGGCTCTAAGTGCTGCTCAGTTAGTGTGTCGAGACGTTGCTGCCATAGATATCAATATGGGTTGCCCTAAGTCATTTTCTATCAGTGGGGGCATGGGTGCGGCACTGTTGAATAAACCAGACCTTATTCATGATATTTTGACAACATTGAGGAGAAACTTGGACGTTCTAATTACCTGTAAGATTCGACTTTTAAATTCACCTCAGGATACAGTGGAGTTAGCTAGACGAATAGAAACTACTGGTGTTTCAGCTCTTGGTGTTCATGGAAGAAGAGTTGCAGACAGACCAAGAGATCCAGCTAAATGGAGTGAGATTGCAGATGTTGTAGCAGCATTGACTATTCCTGTTATTGCAAATGGTGATATCTTTGAGTATGATGATTTTCAACGGATCAAAGTTCATACAGGTGCTTCTTCACTGATGGTTGCTCGAGGAGCTCTTTGGAATGCTTCAATCTTCTCTCCAGAAGGCAAATTACCATGGGAAGATGTGAAAAGAGAATATGTTAGGAAGAGCATCTTGTGGGATAATGATATCAAAAGTACAAAGTATACGCTAAAAGAAATGATAATGCATCATTCTTGTCTTGAACTGCCAGAAGGAAAGGCCGTGATAAAATCTGAATCCCTTGCAGACCTAGCGTAA

Protein sequence

MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGMGAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRRVADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLADLA

Homology

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BLAST of MELO3C007688 vs. NCBI nr
Match: KAA0036369.1 (tRNA-dihydrouridine(20) synthase [Cucumis melo var. makuwa] >TYK12764.1 tRNA-dihydrouridine(20) synthase [Cucumis melo var. makuwa])

HSP 1 Score: 609.4 bits (1570), Expect = 1.7e-170
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120
           KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300

Query: 301 DLA 304
           DLA
Sbjct: 301 DLA 303

[Close]

BLAST of MELO3C007688 vs. NCBI nr
Match: XP_008440492.1 (PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+]-like [Cucumis melo])

HSP 1 Score: 609.4 bits (1570), Expect = 1.7e-170
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120
           KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300

Query: 301 DLA 304
           DLA
Sbjct: 301 DLA 303

[Close]

BLAST of MELO3C007688 vs. NCBI nr
Match: XP_004143428.1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucumis sativus])

HSP 1 Score: 601.7 bits (1550), Expect = 3.6e-168
Identity = 297/303 (98.02%), Postives = 302/303 (99.67%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDYENK VLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE
Sbjct: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120
           KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVC+DVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNL++LITCKIRLLNSPQDTVELARRIE+TGVSALGVHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300

Query: 301 DLA 304
           DLA
Sbjct: 301 DLA 303

[Close]

BLAST of MELO3C007688 vs. NCBI nr
Match: XP_038880913.1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Benincasa hispida])

HSP 1 Score: 582.0 bits (1499), Expect = 2.9e-162
Identity = 286/303 (94.39%), Postives = 298/303 (98.35%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLA+QYGADITYGEEIIDHKMIKCERRLNEH+GSTDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRLNEHLGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120
           KGTNNVVFRTCSLERK VVFQMGTSNAARALSAAQ++C+DVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTNNVVFRTCSLERKRVVFQMGTSNAARALSAAQILCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNLD+ ITCKIRLL S QDTVELARRIETTGVSALGVHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLDIPITCKIRLLKSSQDTVELARRIETTGVSALGVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAALTIPVIANGDIFE+DDFQRIKVHTGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEFDDFQRIKVHTGASSMMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIK TK+TLKEMIMH+SCLELPEGKA+IKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKGTKHTLKEMIMHYSCLELPEGKALIKSESLA 300

Query: 301 DLA 304
           D+A
Sbjct: 301 DIA 303

[Close]

BLAST of MELO3C007688 vs. NCBI nr
Match: XP_022132400.1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Momordica charantia])

HSP 1 Score: 560.8 bits (1444), Expect = 7.0e-156
Identity = 276/303 (91.09%), Postives = 293/303 (96.70%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           M+YENKL+LAPMVRVGTLPFRLLA+QYGADITYGEEIIDHKM+KCERRLNE VGSTDFVE
Sbjct: 1   MEYENKLILAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMVKCERRLNERVGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120
           KGT+NVVFRTCSLER  VVFQMGTSNA RAL+AAQ+VC+DVAA+DINMGCPKSFSISGGM
Sbjct: 61  KGTDNVVFRTCSLERSQVVFQMGTSNAVRALTAAQIVCQDVAAVDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNL++ ITCKIRLL+S QDTVELARRIET GVSAL VHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLNIPITCKIRLLSSSQDTVELARRIETIGVSALVVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAAL+IPVIANGDIFEY DFQR+KV TGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALSIPVIANGDIFEYADFQRVKVDTGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIKSTK+TLKEMIMH+SCLELPEGKAVIKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSESLA 300

Query: 301 DLA 304
           DLA
Sbjct: 301 DLA 303

[Close]

BLAST of MELO3C007688 vs. ExPASy Swiss-Prot
Match: Q9NX74 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Homo sapiens OX=9606 GN=DUS2 PE=1 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.4e-79
Identity = 156/304 (51.32%), Postives = 208/304 (68.42%), Query Frame = 0


Query: 3   YENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKG 62
           Y NKL+LAPMVRVGTLP RLLA  YGADI Y EE+ID KMI+C+R +NE + + DFV   
Sbjct: 10  YHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAP- 69

Query: 63  TNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGMGA 122
            + VVFRTC  E+  VVFQMGTS+A RAL+ A+LV  DVA ID+NMGCPK +S  GGMGA
Sbjct: 70  DDRVVFRTCEREQNRVVFQMGTSDAERALAVARLVENDVAGIDVNMGCPKQYSTKGGMGA 129

Query: 123 ALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRRVA 182
           ALL+ PD I  IL+TL +     +TCKIR+L S +DT+ L +RIE TG++A+ VHGR+  
Sbjct: 130 ALLSDPDKIEKILSTLVKGTRRPVTCKIRILPSLEDTLSLVKRIERTGIAAIAVHGRKRE 189

Query: 183 DRPRDPAKWSEIADVVAALTIPVIANG----DIFEYDDFQRIKVHTGASSLMVARGALWN 242
           +RP+ P     I  +   L+IPVIANG     I +Y D +  +  T ASS+MVAR A+WN
Sbjct: 190 ERPQHPVSCEVIKAIADTLSIPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAAMWN 249

Query: 243 ASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSES 302
            SIF  EG  P E+V ++Y+R ++ +DN   +TKY L +M+     LE P+G+ +  ++S
Sbjct: 250 PSIFLKEGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQ--LESPQGRLLHAAQS 309

[Close]

BLAST of MELO3C007688 vs. ExPASy Swiss-Prot
Match: Q9D7B1 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Mus musculus OX=10090 GN=Dus2 PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 2.7e-78
Identity = 154/304 (50.66%), Postives = 207/304 (68.09%), Query Frame = 0


Query: 3   YENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKG 62
           Y NKL+LAPMVRVGTLP RLLA  YGADI Y EE+ID KM++C+R +NE + + DFV   
Sbjct: 10  YHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMLQCKRVVNEVLSTVDFVAP- 69

Query: 63  TNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGMGA 122
            + VVFRTC  E+  VVFQMGTS+A RAL+ A+LV  DVA ID+NMGCPK +S  GGMGA
Sbjct: 70  DDRVVFRTCEREQSRVVFQMGTSDAERALAVARLVENDVAGIDVNMGCPKEYSTKGGMGA 129

Query: 123 ALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRRVA 182
           ALL+ PD I  IL+TL +     +TCKIR+L S +DT+ L +RIE TG+SA+ VHGR   
Sbjct: 130 ALLSDPDKIEKILSTLVKGTHRPVTCKIRILPSLEDTLNLVKRIERTGISAIAVHGRNRD 189

Query: 183 DRPRDPAKWSEIADVVAALTIPVIANG----DIFEYDDFQRIKVHTGASSLMVARGALWN 242
           +RP+ P     I  +   L+IPVIANG     I ++ D +  +  T ASS+MVAR A+WN
Sbjct: 190 ERPQHPVSCEVIRAIAETLSIPVIANGGSHDHIQQHVDIEDFRQATAASSVMVARAAMWN 249

Query: 243 ASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSES 302
            SIF  +G  P E+V ++Y+R ++ +DN   +TKY L +M+     LE P+G+ +  ++S
Sbjct: 250 PSIFLKDGLRPLEEVMQKYIRYAVQYDNHYTNTKYCLCQMLREQ--LESPQGRLLHAAQS 309

[Close]

BLAST of MELO3C007688 vs. ExPASy Swiss-Prot
Match: O74731 (tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dus2 PE=3 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.3e-53
Identity = 112/289 (38.75%), Postives = 172/289 (59.52%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIK---CERRLNEHVGSTD 60
           ++Y NK+ LAPMVR+G LP RLLA +YGA++ +G EI+D  ++     ER +N+ +   D
Sbjct: 4   LNYSNKVCLAPMVRIGELPMRLLALRYGANLVWGPEIVDKALLSGTPVERVVNDRINCID 63

Query: 61  FVEKGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSIS 120
           FV+  +N V+FR   LE   ++FQ+G+++   A+ AA+LV  DVA ID+N GCPK FS+ 
Sbjct: 64  FVKPPSNKVLFRVHPLEANRLIFQLGSASPELAVEAAKLVANDVAGIDLNCGCPKHFSVH 123

Query: 121 GGMGAALLNKPDLIHDILTTLRRNL----DVLITCKIRLLNSPQDTVELARRIETTGVSA 180
            GMGA LL   D +  IL  L   +     + I+CKIRLL + +DT++L  RI  TGV A
Sbjct: 124 AGMGAGLLKNQDRLVSILDALVNEIGKPYKISISCKIRLLETKEDTLKLVERICDTGVRA 183

Query: 181 LGVHGRRVADRPRDPAKWSEIADVVAAL---TIPVIANGDIFEYDDFQRIKVHTGASSLM 240
           + VH R    R  +PA  S ++++V       + ++ NGD+  Y+D   +    G   ++
Sbjct: 184 ITVHCRTTPMRNTEPADRSYLSEIVGVCRNKDVSILVNGDVLSYNDGLDVIEKYGVDGVL 243

Query: 241 VARGALWNASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMI 280
           +AR A  N S F  EG L    V  E+++ ++  DN+  +TKY L +++
Sbjct: 244 IARAAERNVSCFRIEGPLSSFKVAEEFLKMALEVDNNFGNTKYCLNQIM 292

[Close]

BLAST of MELO3C007688 vs. ExPASy Swiss-Prot
Match: P53720 (tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SMM1 PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 3.2e-42
Identity = 112/298 (37.58%), Postives = 169/298 (56.71%), Query Frame = 0


Query: 3   YENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDF---- 62
           Y  KLVLAPMVR G LP RL+A  +GAD+ +  EIID K+I+C R+ N  + + D+    
Sbjct: 4   YAGKLVLAPMVRAGELPTRLMALAHGADLVWSPEIIDKKLIQCVRKENTALQTVDYVVPS 63

Query: 63  -VEKGTNNVVFRTC-SLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSI 122
            V+     +VFRT   LE   ++FQ+G+++ A A  AA  V  DV+ IDIN GCPK FSI
Sbjct: 64  KVQTRPETLVFRTYPKLESSKLIFQIGSASPALATQAALKVINDVSGIDINAGCPKHFSI 123

Query: 123 SGGMGAALLNKPDLIHDILTTLRRNL----DVLITCKIRLLNSPQDTVELARRIETTGVS 182
             GMG+ALL  PD +  IL  L +N+       I+ KIRLL++ QDT++L +R+  TG++
Sbjct: 124 HSGMGSALLRTPDTLCLILKELVKNVGNPHSKPISVKIRLLDTKQDTLQLVKRLCATGIT 183

Query: 183 ALGVHGRRVADRPRDPA---KWSEIADVVAALTIPVIANGDIFEYDDFQRIKV-HTGASS 242
            L VH R+   R R+       +EI ++  A  + +I NG I +   F  ++  H   ++
Sbjct: 184 NLTVHCRKTEMRNREQPITDYIAEIYEICQANNVSLIVNGAIRDRSHFHDLQANHWKNTN 243

Query: 243 L--MVARGALWNASIFSPEGK-----LPWEDVKREYVRKSILWDNDIKSTKYTLKEMI 280
           +  M+A  A  + ++F    K       W    RE+++ +  +DN I +TKY L  ++
Sbjct: 244 IGGMIAECAERDPTVFDHTSKPSEDGPSWVVACREFIQWATKFDNHIGNTKYMLSRIV 301

[Close]

BLAST of MELO3C007688 vs. ExPASy Swiss-Prot
Match: Q32M08 (tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS=Mus musculus OX=10090 GN=Dus4l PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 4.7e-30
Identity = 84/258 (32.56%), Postives = 129/258 (50.00%), Query Frame = 0


Query: 8   VLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKGTNNVV 67
           V APMVR   L FR L  +Y  D+ Y   II                + DFV        
Sbjct: 30  VCAPMVRYSKLAFRTLVRKYSCDLCYTPMII----------------AADFVRSIKARDS 89

Query: 68  FRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGMGAALLNK 127
             T +     ++ Q   ++A     AA LVC     IDIN GCP+ ++++ G GA L+NK
Sbjct: 90  EFTTNQGDCPLIVQFAANDARLLSDAALLVCPYANGIDINCGCPQRWAMADGYGACLINK 149

Query: 128 PDLIHDILTTLRRNLD---VLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRRVADR 187
           P+L+HD++  +R  ++     ++ KIR+ +    T++L R+ E TGVS + VHGR V +R
Sbjct: 150 PELVHDMVRQVRNRVESPRFSVSIKIRIHDDLARTIDLCRKAEATGVSWITVHGRTVEER 209

Query: 188 PRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNASIFSP 247
              P  +  I  +   ++IP++ANGDI    + + +   TG   +MVARG L N ++F+ 
Sbjct: 210 -HQPVHYDAIKMIKENVSIPIVANGDIRSLKEAENVWQMTGTDGVMVARGLLANPAMFAG 269

Query: 248 EGKLP------WEDVKRE 257
             + P      W D+  E
Sbjct: 270 YEETPLKCIWDWVDISLE 270

[Close]

BLAST of MELO3C007688 vs. ExPASy TrEMBL
Match: A0A5D3CNX9 (tRNA-dihydrouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003530 PE=3 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 8.3e-171
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120
           KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300

Query: 301 DLA 304
           DLA
Sbjct: 301 DLA 303

[Close]

BLAST of MELO3C007688 vs. ExPASy TrEMBL
Match: A0A1S3B193 (tRNA-dihydrouridine synthase OS=Cucumis melo OX=3656 GN=LOC103484905 PE=3 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 8.3e-171
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120
           KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300

Query: 301 DLA 304
           DLA
Sbjct: 301 DLA 303

[Close]

BLAST of MELO3C007688 vs. ExPASy TrEMBL
Match: A0A0A0KGL3 (tRNA-dihydrouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G497190 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 1.7e-168
Identity = 297/303 (98.02%), Postives = 302/303 (99.67%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDYENK VLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE
Sbjct: 1   MDYENKFVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120
           KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVC+DVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNL++LITCKIRLLNSPQDTVELARRIE+TGVSALGVHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLNILITCKIRLLNSPQDTVELARRIESTGVSALGVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSMMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300

Query: 301 DLA 304
           DLA
Sbjct: 301 DLA 303

[Close]

BLAST of MELO3C007688 vs. ExPASy TrEMBL
Match: A0A6J1BSD4 (tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005266 PE=4 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.4e-156
Identity = 276/303 (91.09%), Postives = 293/303 (96.70%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           M+YENKL+LAPMVRVGTLPFRLLA+QYGADITYGEEIIDHKM+KCERRLNE VGSTDFVE
Sbjct: 1   MEYENKLILAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMVKCERRLNERVGSTDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120
           KGT+NVVFRTCSLER  VVFQMGTSNA RAL+AAQ+VC+DVAA+DINMGCPKSFSISGGM
Sbjct: 61  KGTDNVVFRTCSLERSQVVFQMGTSNAVRALTAAQIVCQDVAAVDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180
           GAALLNKPDLIHDILTTLRRNL++ ITCKIRLL+S QDTVELARRIET GVSAL VHGRR
Sbjct: 121 GAALLNKPDLIHDILTTLRRNLNIPITCKIRLLSSSQDTVELARRIETIGVSALVVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAAL+IPVIANGDIFEY DFQR+KV TGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALSIPVIANGDIFEYADFQRVKVDTGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSPEGKLPWEDVKREYVRKSILWDNDIKSTK+TLKEMIMH+SCLELPEGKAVIKSESLA
Sbjct: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSESLA 300

Query: 301 DLA 304
           DLA
Sbjct: 301 DLA 303

[Close]

BLAST of MELO3C007688 vs. ExPASy TrEMBL
Match: A0A6J1KSI8 (tRNA-dihydrouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111497209 PE=3 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 2.9e-155
Identity = 274/303 (90.43%), Postives = 291/303 (96.04%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDYENKLVLAPMVRVGTLPFRLLA+QYGADITYGEEIIDHKMIKCERR+NEHVG+ DFVE
Sbjct: 1   MDYENKLVLAPMVRVGTLPFRLLAAQYGADITYGEEIIDHKMIKCERRINEHVGAVDFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120
           KGT+NVVFRTCSLE+  VVFQMGTSNA RALSAAQLVC+DVAAIDINMGCPKSFSISGGM
Sbjct: 61  KGTDNVVFRTCSLEKNQVVFQMGTSNAVRALSAAQLVCQDVAAIDINMGCPKSFSISGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180
           GAALL KPDLIHDILTTLRRNLD+ ITCKIRLLNS QDTVELARRIETTGVSAL VHGRR
Sbjct: 121 GAALLKKPDLIHDILTTLRRNLDIPITCKIRLLNSSQDTVELARRIETTGVSALAVHGRR 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240
           VADRPRDPAKWSEIADVVAAL++PVIANGDIFEY+DF  IKV TGASS+MVARGALWNAS
Sbjct: 181 VADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGASSVMVARGALWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFS +GKLPWE+VKREYVRKSILWDNDIKSTK+TLKEMIMH+SCLELPEGKAVIKS++LA
Sbjct: 241 IFSEDGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLA 300

Query: 301 DLA 304
           DLA
Sbjct: 301 DLA 303

[Close]

BLAST of MELO3C007688 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 491.5 bits (1264), Expect = 4.9e-139
Identity = 232/303 (76.57%), Postives = 271/303 (89.44%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           MDY+NKLVLAPMVRVGTL FR+LA++YGADITYGEEIIDHK++KCERRLN   G+++FVE
Sbjct: 1   MDYQNKLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVE 60

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGM 120
           KGT+NVVF TC  E+  VVFQMGTS+A RAL A+++VC DVA IDINMGCPK+FSI GGM
Sbjct: 61  KGTDNVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGM 120

Query: 121 GAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR 180
           GAALL+KP+LIHDIL TL+RNLDV +TCKIRLL SP DTVELARRIE  GV AL VHGR+
Sbjct: 121 GAALLSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHGRK 180

Query: 181 VADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNAS 240
           +ADRPRDPAKW EIADVVAAL+IPVIANGD+ EYDDF RIK  TGA+S+MVARGA+WNAS
Sbjct: 181 IADRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNAS 240

Query: 241 IFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAVIKSESLA 300
           IFSP+GK  WEDVK++Y+RKSILW+ND+KSTKYT+KEMI HHSCLELPEGK++ K+++L 
Sbjct: 241 IFSPKGKSHWEDVKKKYLRKSILWNNDVKSTKYTIKEMIAHHSCLELPEGKSINKADTLE 300

Query: 301 DLA 304
           DLA
Sbjct: 301 DLA 303

[Close]

BLAST of MELO3C007688 vs. TAIR 10
Match: AT3G49640.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 400.2 bits (1027), Expect = 1.5e-111
Identity = 189/250 (75.60%), Postives = 222/250 (88.80%), Query Frame = 0


Query: 54  GSTDFVEKGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKS 113
           G+++FVEKGT+NVVF TC  E+  VVFQMGTS+A RAL A+++VC DVA IDINMGCPK+
Sbjct: 15  GTSEFVEKGTDNVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKA 74

Query: 114 FSISGGMGAALLNKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSA 173
           FSI GGMGAALL+KP+LIHDIL TL+RNLDV +TCKIRLL SP DTVELARRIE  GV A
Sbjct: 75  FSIQGGMGAALLSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPA 134

Query: 174 LGVHGRRVADRPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVAR 233
           L VHGR++ADRPRDPAKW EIADVVAAL+IPVIANGD+ EYDDF RIK  TGA+S+MVAR
Sbjct: 135 LAVHGRKIADRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVAR 194

Query: 234 GALWNASIFSPEGKLPWEDVKREYVRKSILWDNDIKSTKYTLKEMIMHHSCLELPEGKAV 293
           GA+WNASIFSP+GK  WEDVK++Y+RKSILW+ND+KSTKYT+KEMI HHSCLELPEGK++
Sbjct: 195 GAMWNASIFSPKGKSHWEDVKKKYLRKSILWNNDVKSTKYTIKEMIAHHSCLELPEGKSI 254

Query: 294 IKSESLADLA 304
            K+++L DLA
Sbjct: 255 NKADTLEDLA 264

[Close]

BLAST of MELO3C007688 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 2.9e-27
Identity = 78/247 (31.58%), Postives = 120/247 (48.58%), Query Frame = 0


Query: 6   KLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVEKGTNN 65
           K ++APMV    LPFRLL  +YGA   Y   +      + E+  N+              
Sbjct: 89  KYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETEKYRNQE------------- 148

Query: 66  VVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRDVAAIDINMGCPKSFSISGGMGAALL 125
             F TC  +R   V Q   ++    L AA+ V      +DIN+GCP+  +  G  GA L+
Sbjct: 149 --FTTCKEDRPLFV-QFCANDPDTLLEAAKRVEPYCDYVDINLGCPQRIARRGNYGAFLM 208

Query: 126 NKPDLIHDILTTLRRNLDVLITCKIRLLNSPQDTVELARRIETTGVSALGVHGRR--VAD 185
           +   L+  ++  L +NL+V ++CKIR+  + +DT++ A+ +E  G S L VHGR     D
Sbjct: 209 DNLPLVKSLVEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDAGCSLLAVHGRTRDEKD 268

Query: 186 RPRDPAKWSEIADVVAALTIPVIANGDIFEYDDFQRIKVHTGASSLMVARGALWNASIFS 245
             +  A WS I +V  A+ IPV+ANG++   +D       TG   ++ A   L N + F+
Sbjct: 269 GKKFRADWSAIKEVKNAMRIPVLANGNVRCIEDVDNCIKETGVEGVLSAETLLENPAAFA 319

Query: 246 PEGKLPW 251
                 W
Sbjct: 329 GFRTAEW 319

[Close]

BLAST of MELO3C007688 vs. TAIR 10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 105.5 bits (262), Expect = 7.5e-23
Identity = 85/290 (29.31%), Postives = 129/290 (44.48%), Query Frame = 0


Query: 1   MDYENKLVLAPMVRVGTLPFRLLASQYGADITYGEEIIDHKMIKCERRLNEHVGSTDFVE 60
           +D+ +KL LAP+  VG LPFR L    GAD+T GE      M  C   L         + 
Sbjct: 334 IDFRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGE------MAMCTNLLQGQASEWALLR 393

Query: 61  KGTNNVVFRTCSLERKHVVFQMGTSNAARALSAAQLVCRD--VAAIDINMGCPKSFSISG 120
           + ++  +F            Q+  S         +L+ R+  V  IDINMGCP    ++ 
Sbjct: 394 RHSSEDLFGV----------QICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNK 453

Query: 121 GMGAALLNKPDLIHDILTTLRRNLDVLITCKIR--LLNSPQDTVELARRIETTGVSALGV 180
             G+ALLNKP  + +I+      ++  IT K+R            L   I   G +A+ +
Sbjct: 454 SAGSALLNKPLRMKNIVEVSSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTI 513

Query: 181 HGRRVADRPRDPAKWSEIADVV--AALTIPVIANGDIFEYDDFQRIKVH-TGASSLMVAR 240
           HGR    R    A W  I      A   + VI NGD++ Y D+ + K      SS M+AR
Sbjct: 514 HGRSRQQRYSKSADWDYIYQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCPELSSCMIAR 573

Query: 241 GALWNASIFSP-EGKLPWE-------DVKREYVRKSIL-WDNDIKSTKYT 275
           GAL    IF+  + +  W+       ++ +++VR  +  W +D K  + T
Sbjct: 574 GALIKPWIFTEIKEQRHWDITSGERLNIMKDFVRFGLQHWGSDTKGVETT 607

The following BLAST results are available for this feature:

BLAST of MELO3C007688 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0036369.1	1.7e-170	100.00	tRNA-dihydrouridine(20) synthase [Cucumis melo var. makuwa] >TYK12764.1 tRNA-dih...	[more]
XP_008440492.1	1.7e-170	100.00	PREDICTED: tRNA-dihydrouridine(20) synthase [NAD(P)+]-like [Cucumis melo]	[more]
XP_004143428.1	3.6e-168	98.02	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucumis sativus]	[more]
XP_038880913.1	2.9e-162	94.39	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Benincasa hispida]	[more]
XP_022132400.1	7.0e-156	91.09	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Momordica charantia]	[more]

BLAST of MELO3C007688 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9NX74	1.4e-79	51.32	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Homo sapiens OX=9606 GN=DUS2 ...	[more]
Q9D7B1	2.7e-78	50.66	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like OS=Mus musculus OX=10090 GN=Dus2...	[more]
O74731	2.3e-53	38.75	tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Schizosaccharomyces pombe (strain ...	[more]
P53720	3.2e-42	37.58	tRNA-dihydrouridine(20) synthase [NAD(P)+] OS=Saccharomyces cerevisiae (strain A...	[more]
Q32M08	4.7e-30	32.56	tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like OS=Mus musculus OX=10090 GN...	[more]

BLAST of MELO3C007688 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CNX9	8.3e-171	100.00	tRNA-dihydrouridine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3B193	8.3e-171	100.00	tRNA-dihydrouridine synthase OS=Cucumis melo OX=3656 GN=LOC103484905 PE=3 SV=1	[more]
A0A0A0KGL3	1.7e-168	98.02	tRNA-dihydrouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G497190 PE=3 SV=...	[more]
A0A6J1BSD4	3.4e-156	91.09	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 OS=Momordica charanti...	[more]
A0A6J1KSI8	2.9e-155	90.43	tRNA-dihydrouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111497209 PE=3 SV...	[more]

BLAST of MELO3C007688 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G49640.1	4.9e-139	76.57	Aldolase-type TIM barrel family protein	[more]
AT3G49640.2	1.5e-111	75.60	Aldolase-type TIM barrel family protein	[more]
AT5G67220.1	2.9e-27	31.58	FMN-linked oxidoreductases superfamily protein	[more]
AT4G38890.1	7.5e-23	29.31	FMN-linked oxidoreductases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001269	tRNA-dihydrouridine synthase	PIRSF	PIRSF006621	Dus	coord: 1..293 e-value: 4.5E-56 score: 188.1
IPR035587	DUS-like, FMN-binding domain	PFAM	PF01207	Dus	coord: 8..256 e-value: 2.3E-54 score: 184.6
IPR035587	DUS-like, FMN-binding domain	CDD	cd02801	DUS_like_FMN	coord: 6..250 e-value: 1.1094E-94 score: 277.839
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 1..248 e-value: 1.5E-74 score: 252.3
None	No IPR available	PIRSR	PIRSR006621-1	PIRSR006621-1	coord: 3..256 e-value: 6.1E-56 score: 187.4
None	No IPR available	PANTHER	PTHR45936	TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE	coord: 1..302
None	No IPR available	SUPERFAMILY	51395	FMN-linked oxidoreductases	coord: 5..281
IPR018517	tRNA-dihydrouridine synthase, conserved site	PROSITE	PS01136	UPF0034	coord: 104..122

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C007688.1	MELO3C007688.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008033	tRNA processing
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0017150	tRNA dihydrouridine synthase activity