MELO3C007311.jh1 (gene) Melon (Harukei-3) v1.41

BLAST of MELO3C007311.jh1 vs. NCBI nr
Match: XP_008439896.1 (PREDICTED: sphingosine kinase 1 [Cucumis melo] >KAA0052712.1 sphingosine kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1001 bits (2587), Expect = 0.0
Identity = 491/491 (100.00%), Postives = 491/491 (100.00%), Query Frame = 0

Query: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60
           MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR
Sbjct: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60

Query: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120
           IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN
Sbjct: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120

Query: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180
           PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG
Sbjct: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180

Query: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240
           ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240

Query: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300
           CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG
Sbjct: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300

Query: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360
           GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD
Sbjct: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360

Query: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP 420
           GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP
Sbjct: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP 420

Query: 421 FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ 480
           FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ
Sbjct: 421 FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ 480

Query: 481 GLATLFSPCRN 491
           GLATLFSPCRN
Sbjct: 481 GLATLFSPCRN 491

BLAST of MELO3C007311.jh1 vs. NCBI nr
Match: XP_004134960.1 (sphingosine kinase 1 [Cucumis sativus] >KGN49199.1 hypothetical protein Csa_004342 [Cucumis sativus])

HSP 1 Score: 969 bits (2506), Expect = 0.0
Identity = 475/491 (96.74%), Postives = 481/491 (97.96%), Query Frame = 0

Query: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60
           MDQS DSD ILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLS+DKEVLGFSMDGPKIR
Sbjct: 1   MDQSGDSDFILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSVDKEVLGFSMDGPKIR 60

Query: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120
           IKALVED GGLRCFG+SGALVRKEFVFQPLSEESRALWCLKLRECIDL+GRPKKLFVLVN
Sbjct: 61  IKALVEDHGGLRCFGSSGALVRKEFVFQPLSEESRALWCLKLRECIDLIGRPKKLFVLVN 120

Query: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180
           PFGGKG GSKIYRDEVKPILEDAEI+VTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG
Sbjct: 121 PFGGKGTGSKIYRDEVKPILEDAEIDVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180

Query: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240
           ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTA NATLAIVRGHK
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTACNATLAIVRGHK 240

Query: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300
           CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG
Sbjct: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300

Query: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360
           GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDG PIS QRHGYEGPNINLKDLEWRKFD
Sbjct: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGEPISIQRHGYEGPNINLKDLEWRKFD 360

Query: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP 420
           GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDC KLSLLSLMTELNNGKHVKSP
Sbjct: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCSKLSLLSLMTELNNGKHVKSP 420

Query: 421 FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ 480
           FVTYIKVKAF+LKPGTRV+EPSKEGIIDADGEILALGKGTLTT QKTLMNYDEL ISVHQ
Sbjct: 421 FVTYIKVKAFILKPGTRVKEPSKEGIIDADGEILALGKGTLTTSQKTLMNYDELLISVHQ 480

Query: 481 GLATLFSPCRN 491
           GLATLFSPCRN
Sbjct: 481 GLATLFSPCRN 491

BLAST of MELO3C007311.jh1 vs. NCBI nr
Match: XP_038883381.1 (sphingosine kinase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 929 bits (2402), Expect = 0.0
Identity = 448/491 (91.24%), Postives = 469/491 (95.52%), Query Frame = 0

Query: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60
           MDQS DS+S+LNHRVLLPDG VTP+ILT+DGWL+WSEK QRRLS++KEVLGFSMDGPKIR
Sbjct: 1   MDQSGDSESVLNHRVLLPDGIVTPLILTSDGWLRWSEKGQRRLSVEKEVLGFSMDGPKIR 60

Query: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120
           IKALVEDRGGLRCFG++GAL RKEFVFQPLSEESRALWCLKLRECIDLLGRPK+LFVLVN
Sbjct: 61  IKALVEDRGGLRCFGSNGALTRKEFVFQPLSEESRALWCLKLRECIDLLGRPKRLFVLVN 120

Query: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180
           PFGGK AG KIY D VKPILEDAEI+VT+QETKYQRHAEEVAYSLD T YDGIVCVSGDG
Sbjct: 121 PFGGKRAGCKIYLDNVKPILEDAEIDVTMQETKYQRHAEEVAYSLDLTKYDGIVCVSGDG 180

Query: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240
           ILVEVINGLLRRDDW DAIK PLGVVPAGTGNGM KSLLH  GDPCTA NATLAIVRGHK
Sbjct: 181 ILVEVINGLLRRDDWADAIKMPLGVVPAGTGNGMAKSLLHLTGDPCTACNATLAIVRGHK 240

Query: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300
           CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLD YALQRIISLRHY+G
Sbjct: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDFYALQRIISLRHYKG 300

Query: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360
           G+SFVPAPGFEDYGEPTRYDYETASVVEVDKSDG PI+ QRHGYEGPNINLKDL+WRKFD
Sbjct: 301 GISFVPAPGFEDYGEPTRYDYETASVVEVDKSDGEPINIQRHGYEGPNINLKDLDWRKFD 360

Query: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP 420
           GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMT+LNNGKHVKSP
Sbjct: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTQLNNGKHVKSP 420

Query: 421 FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ 480
           FVTYIKVKAF+LKPGTRV+EPSKEGIID DGEILA G+GTLTT QKTLMNYD+LQISVHQ
Sbjct: 421 FVTYIKVKAFILKPGTRVKEPSKEGIIDVDGEILAFGRGTLTTNQKTLMNYDKLQISVHQ 480

Query: 481 GLATLFSPCRN 491
           GLATLFSPCRN
Sbjct: 481 GLATLFSPCRN 491

BLAST of MELO3C007311.jh1 vs. NCBI nr
Match: TYK13112.1 (sphingosine kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 904 bits (2336), Expect = 0.0
Identity = 456/497 (91.75%), Postives = 466/497 (93.76%), Query Frame = 0

Query: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60
           MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR
Sbjct: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60

Query: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120
           IKALVEDRGG RCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN
Sbjct: 61  IKALVEDRGGFRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120

Query: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180
           PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG
Sbjct: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180

Query: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240
           ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240

Query: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300
           CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG
Sbjct: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300

Query: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360
           GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD
Sbjct: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360

Query: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGF---LDLII---IRDCPKLSLLSLMTELNNG 420
           GPFISVWLHNVPWGAENTLAAPDAK    +   L +II   +++ P  +  S++ E  + 
Sbjct: 361 GPFISVWLHNVPWGAENTLAAPDAKDPPIWMPRLKVIIEPPVKENPIPNDSSIVFE-KSS 420

Query: 421 KHVKSPFVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDEL 480
                P     +VKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDEL
Sbjct: 421 LESNDPN----RVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDEL 480

Query: 481 QISVHQGLATLFSPCRN 491
           QISVHQGLATLFSPCRN
Sbjct: 481 QISVHQGLATLFSPCRN 492

BLAST of MELO3C007311.jh1 vs. NCBI nr
Match: XP_022978376.1 (sphingosine kinase 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 892 bits (2306), Expect = 0.0
Identity = 430/491 (87.58%), Postives = 460/491 (93.69%), Query Frame = 0

Query: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60
           MDQS DS  ILNHRVLLPDG+VTPMILT DG L+W E+ QR LS+DKEVLGFS DGP IR
Sbjct: 1   MDQSGDSKPILNHRVLLPDGSVTPMILTADGCLRWLEEGQRSLSVDKEVLGFSTDGPNIR 60

Query: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120
           IKALVED GGL CFG+SG LVRKEFVFQPLSEESRALWCLKLREC+DLLGRPK+LFV VN
Sbjct: 61  IKALVEDLGGLLCFGSSGGLVRKEFVFQPLSEESRALWCLKLRECVDLLGRPKRLFVFVN 120

Query: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180
           PFGG+ AG KIY D+VKPIL+DAEI VTLQETKYQRHAEEVAYSLD T YDGIVCVSGDG
Sbjct: 121 PFGGRRAGYKIYLDDVKPILDDAEIVVTLQETKYQRHAEEVAYSLDLTEYDGIVCVSGDG 180

Query: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240
           ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGM KSLLHSIGDPCTA NATLAI+RGHK
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMAKSLLHSIGDPCTACNATLAIIRGHK 240

Query: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300
           CSLDVATISQGE KHF+VLMLAWGL+ADIDIESEKYRWMGSARLD YALQRIISLRHY+G
Sbjct: 241 CSLDVATISQGEVKHFSVLMLAWGLIADIDIESEKYRWMGSARLDFYALQRIISLRHYKG 300

Query: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360
           GVSFVPAPGFEDYGEPTRYDYETASVV+VDKSDG   + +RHGYEGPNINLKDL WRKFD
Sbjct: 301 GVSFVPAPGFEDYGEPTRYDYETASVVDVDKSDGETDNIRRHGYEGPNINLKDLNWRKFD 360

Query: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP 420
           GPFISVWLHNVPWGAENT+AAPDAKMSDGFLDLIIIRDCPKLSLL+LMTELNNG+HVKSP
Sbjct: 361 GPFISVWLHNVPWGAENTMAAPDAKMSDGFLDLIIIRDCPKLSLLALMTELNNGRHVKSP 420

Query: 421 FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ 480
           FVTYIKVKAF+L PGTRVEEP++EGIIDADGE+LALG+ T+T+GQKTLMNYD+LQIS+HQ
Sbjct: 421 FVTYIKVKAFILTPGTRVEEPNREGIIDADGEVLALGRETITSGQKTLMNYDKLQISMHQ 480

Query: 481 GLATLFSPCRN 491
           GLATLFSPCR+
Sbjct: 481 GLATLFSPCRD 491

BLAST of MELO3C007311.jh1 vs. ExPASy Swiss-Prot
Match: Q8L7L1 (Sphingosine kinase 1 OS=Arabidopsis thaliana OX=3702 GN=SPHK1 PE=1 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 4.3e-174
Identity = 292/482 (60.58%), Postives = 374/482 (77.59%), Query Frame = 0

Query: 7   SDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIRIKALVE 66
           S +I++ RVL+ +G VTP+ LT +G L+ +E  +R+ ++ KE+L F ++G K+R+K LVE
Sbjct: 14  SPAIISDRVLV-NGVVTPLTLTAEGELRSTESGRRKSTLAKEILSFVVEGNKVRVKTLVE 73

Query: 67  DRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVNPFGGKG 126
             GG+ C G++G   R +FVF+PLS+ESR LW  K  + +  LGRPKKL V VNPFGGK 
Sbjct: 74  KGGGICCRGSAGDYARNDFVFEPLSDESRKLWSDKFHQHLVSLGRPKKLLVFVNPFGGKK 133

Query: 127 AGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDGILVEVI 186
              KI+++EVKP+ EDA I + +QETKYQ HA+E+  S+D + YDGIVCVSGDGILVEV+
Sbjct: 134 TARKIFQEEVKPLFEDANIQLEIQETKYQLHAKEIVRSMDVSKYDGIVCVSGDGILVEVV 193

Query: 187 NGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHKCSLDVA 246
           NGLL R+DW  AIK P+G+VPAG+GNGM+KSLL  +G PC+A +AT++I+RG   SLDVA
Sbjct: 194 NGLLEREDWKTAIKLPIGMVPAGSGNGMIKSLLEPVGLPCSATSATISIIRGRTRSLDVA 253

Query: 247 TISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRGGVSFVP 306
           TISQG  K F+VLMLAWGLVADIDIESEK+RWMGSAR DIY LQRII LR Y G + FVP
Sbjct: 254 TISQGTTKFFSVLMLAWGLVADIDIESEKFRWMGSARFDIYGLQRIICLRQYHGRILFVP 313

Query: 307 APGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFDGPFISV 366
           APGFE YG+         +   +DK      S +   Y+GP+  L++L+WR+  GPF+SV
Sbjct: 314 APGFESYGQ--------RASCSIDKEPSG--SDKTLVYQGPDSKLENLDWREMKGPFVSV 373

Query: 367 WLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSPFVTYIK 426
           WLHNVPWGAENTLAAPDAK SDGFLDLI+++DCPKL+LLSLMT+L++G HV+SP+ +Y+K
Sbjct: 374 WLHNVPWGAENTLAAPDAKFSDGFLDLIVMKDCPKLALLSLMTKLSDGTHVQSPYASYLK 433

Query: 427 VKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQGLATLF 486
           VKAFVL+PG R++EP KEGIID+DGE+LA G+ +    QK LM+YD+LQISV QGLATLF
Sbjct: 434 VKAFVLEPGARIDEPDKEGIIDSDGEVLARGRKSYKCDQKALMSYDKLQISVDQGLATLF 484

Query: 487 SP 489
           SP
Sbjct: 494 SP 484

BLAST of MELO3C007311.jh1 vs. ExPASy Swiss-Prot
Match: F2Y4A3 (Sphingosine kinase 2 OS=Arabidopsis thaliana OX=3702 GN=SPHK2 PE=1 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 8.7e-167
Identity = 278/476 (58.40%), Postives = 365/476 (76.68%), Query Frame = 0

Query: 15  VLLPDGTVTPMILTTDGWLQWSEKSQRR-LSIDKEVLGFSMDGPKIRIKALVE-DRGGLR 74
           ++  DG +  + LT DG L+W+E   R+ LS+ K+VLGF ++G +IR+KA+VE + GG+ 
Sbjct: 16  IVFIDGELAMLTLTADGELRWTEYGLRQYLSMKKDVLGFIVEGKQIRVKAVVEKEAGGIC 75

Query: 75  CFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVNPFGGKGAGSKIY 134
           C    G  VRK+FVF+PL +++   WC KLR+ +D LGRPK+L V VNPFGGK +  +I+
Sbjct: 76  CGQFGGDFVRKDFVFEPLIDQNG--WCYKLRQYLDSLGRPKRLLVFVNPFGGKKSAREIF 135

Query: 135 RDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDGILVEVINGLLRR 194
             EVKP+ EDA++ + +QETKYQ HA+E   S+D + YDGIVCVSGDGILVEV+NGLL R
Sbjct: 136 VKEVKPLFEDADVQLEIQETKYQLHAKEFVKSMDVSKYDGIVCVSGDGILVEVVNGLLER 195

Query: 195 DDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHKCSLDVATISQGE 254
            DW +A+K P+G+VPAGTGNGM+KSLL ++G  C A +AT++I+RGHK S+DVATI+QG 
Sbjct: 196 ADWRNALKLPIGMVPAGTGNGMIKSLLDTVGLRCCANSATISIIRGHKRSVDVATIAQGN 255

Query: 255 AKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRGGVSFVPAPGFED 314
            K F+VLMLAWGL+ADIDIESEK+RWMGSAR+D YALQRII LR Y G + F+PAPGFE 
Sbjct: 256 TKFFSVLMLAWGLIADIDIESEKFRWMGSARIDFYALQRIICLRRYNGRILFLPAPGFEG 315

Query: 315 YGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFDGPFISVWLHNVP 374
           YG+P      + S+ +        +S +  GY+GP    +DLEWR+  GPF+++WLHNVP
Sbjct: 316 YGQPA-----SCSLYQEPH-----VSDKEVGYQGPETKFEDLEWREMKGPFVTIWLHNVP 375

Query: 375 WGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSPFVTYIKVKAFVL 434
           WG+ENTL AP AK SDG+LDLI++++CPKL LLSLM + ++G HV+SP++ YIKVKAFVL
Sbjct: 376 WGSENTLTAPAAKFSDGYLDLIVLKNCPKLVLLSLMRQTSSGTHVESPYIVYIKVKAFVL 435

Query: 435 KPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQGLATLFSP 489
           +PG  V+EP KEGIID+DGE+LA GK T    QK LM+YD+LQ++V QGLATLFSP
Sbjct: 436 EPGALVDEPDKEGIIDSDGEVLARGKRTYKCDQKALMSYDKLQVTVDQGLATLFSP 479

BLAST of MELO3C007311.jh1 vs. ExPASy Swiss-Prot
Match: Q86KF9 (Sphingosine kinase A OS=Dictyostelium discoideum OX=44689 GN=sgkA PE=2 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 1.8e-50
Identity = 132/443 (29.80%), Postives = 210/443 (47.40%), Query Frame = 0

Query: 111 RPKKLFVLVNPFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNY 170
           R +++ V++NP  GK     I++D +  + +D++I V    TK   HA+++ Y  +   Y
Sbjct: 179 RERRIRVILNPKSGKKMSDSIFKD-INELFKDSKIFVKKTVTKGPDHAKKIGYKFNLKKY 238

Query: 171 DGIVCVSGDGILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARN 230
           D IV +SGDG+  E INGLL R D+  A K PL ++P GTGNG+  S+   + DP    +
Sbjct: 239 DTIVFISGDGLFHEFINGLLSRTDFEQARKIPLALIPGGTGNGIACSI--GLQDP---MS 298

Query: 231 ATLAIVRGHKCSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQ 290
             LA++RG    LDV+ I QG+ K  ++L L WG+V+D+DIESEKYR +G  RL + A  
Sbjct: 299 CALAVIRGFTKPLDVSVIQQGDKKWCSILSLTWGIVSDVDIESEKYRALGDVRLILGAAL 358

Query: 291 RIISLRHYRGGVSFVPA-----------PGFEDYGEPTRYDYETASVVEV---------- 350
           RI++LR YRG + ++PA           P      E    D   +++  V          
Sbjct: 359 RILNLRIYRGKIWYLPALELNKTEIAKIPKCSYSCEICESDNPISTIENVISQQQQNNTT 418

Query: 351 --------------------------------------DKSDGVPISTQRHGYEGPNI-- 410
                                                  K + + I  + H ++  ++  
Sbjct: 419 TNQLRRSSLNNSSNGFSPKLNTSGSISNGNDDELRSMFSKEENIKIENEIHDHQNDDVLP 478

Query: 411 ---------------------NLKDLE-------WRKFDGPFISVWLHNVPWGAENTLAA 462
                                 LK+ +       W+  +G FI +    V   A + +A+
Sbjct: 479 LLKSSSDQHFIVPPTKLPNQQFLKETKEELFAKGWKVLEGEFIGIVASTVSHLASDFIAS 538

BLAST of MELO3C007311.jh1 vs. ExPASy Swiss-Prot
Match: Q9NYA1 (Sphingosine kinase 1 OS=Homo sapiens OX=9606 GN=SPHK1 PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 7.1e-44
Identity = 119/358 (33.24%), Postives = 187/358 (52.23%), Query Frame = 0

Query: 108 LLGRPKKLFVLVNPFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDF 167
           +L RP ++ VL+NP GGKG   +++R  V+P+L +AEI+ TL  T+ + HA E+  S + 
Sbjct: 10  VLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEEL 69

Query: 168 TNYDGIVCVSGDGILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIG---- 227
             +D +V +SGDG++ EV+NGL+ R DW  AI+ PL  +PAG+GN +  SL H  G    
Sbjct: 70  GRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQV 129

Query: 228 -DPCTARNATLAIVRGHKCSLDVATISQGEA-KHFTVLMLAWGLVADIDIESEKYRWMGS 287
            +     N TL + R     +++ ++      + F+VL LAWG +AD+D+ESEKYR +G 
Sbjct: 130 TNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGE 189

Query: 288 ARLDIYALQRIISLRHYRGGVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQR 347
            R  +    R+ +LR YRG ++++P       G  T      AS V V +    P+    
Sbjct: 190 MRFTLGTFLRLAALRTYRGRLAYLPV---GRVGSKT-----PASPVVVQQG---PVDAHL 249

Query: 348 HGYEGPNINLKDLEWRKF-DGPFISVW-LHNVPWGAENTLAAPDAKMSDGFLDLIIIR-D 407
              E P        W    D  F+ V  L +   G+E   AAP  + + G + L  +R  
Sbjct: 250 VPLEEP----VPSHWTVVPDEDFVLVLALLHSHLGSE-MFAAPMGRCAAGVMHLFYVRAG 309

Query: 408 CPKLSLLSLMTELNNGKHV--KSPFVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEIL 455
             +  LL L   +  G+H+  + P++ Y+ V AF      R+E    +G+   DGE++
Sbjct: 310 VSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAF------RLEPKDGKGVFAVDGELM 345

BLAST of MELO3C007311.jh1 vs. ExPASy Swiss-Prot
Match: Q8CI15 (Sphingosine kinase 1 OS=Mus musculus OX=10090 GN=Sphk1 PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 5.1e-42
Identity = 113/359 (31.48%), Postives = 182/359 (50.70%), Query Frame = 0

Query: 108 LLGRPKKLFVLVNPFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDF 167
           LL RP ++ VL+NP GGKG   ++++  V+P LE+AEI   L  T+ + HA E+  + + 
Sbjct: 10  LLPRPCRVLVLLNPQGGKGKALQLFQSRVQPFLEEAEITFKLILTERKNHARELVCAEEL 69

Query: 168 TNYDGIVCVSGDGILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCT 227
            ++D +  +SGDG++ EV+NGL+ R DW  AI+ PL  +P G+GN +  S+ H  G    
Sbjct: 70  GHWDALAVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPGGSGNALAASVNHYAGYEQV 129

Query: 228 AR-----NATLAIVRGHKCSLDVATISQGEA-KHFTVLMLAWGLVADIDIESEKYRWMGS 287
                  N TL + R     +++ ++      + ++VL L+WG VAD+D+ESEKYR +G 
Sbjct: 130 TNEDLLINCTLLLCRRRLSPMNLLSLHTASGLRLYSVLSLSWGFVADVDLESEKYRRLGE 189

Query: 288 ARLDIYALQRIISLRHYRGGVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQR 347
            R  +    R+ SLR Y+G ++++P          T      AS + V K    P+ T  
Sbjct: 190 IRFTVGTFFRLASLRIYQGQLAYLPV--------GTVASKRPASTL-VQKG---PVDTHL 249

Query: 348 HGYEGPNINLKDLEWR---KFDGPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIR- 407
              E P        W    + D   + V LH     +    AAP  +   G + L  +R 
Sbjct: 250 VPLEEP----VPSHWTVVPEQDFVLVLVLLHT--HLSSELFAAPMGRCEAGVMHLFYVRA 309

Query: 408 DCPKLSLLSLMTELNNGKHVK--SPFVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEIL 455
              + +LL L   +  GKH++   P++ ++ V AF      R+E  S+ G+   DGE++
Sbjct: 310 GVSRAALLRLFLAMQKGKHMELDCPYLVHVPVVAF------RLEPRSQRGVFSVDGELM 344

BLAST of MELO3C007311.jh1 vs. ExPASy TrEMBL
Match: A0A5A7UAC8 (Sphingosine kinase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G003250 PE=4 SV=1)

HSP 1 Score: 1001 bits (2587), Expect = 0.0
Identity = 491/491 (100.00%), Postives = 491/491 (100.00%), Query Frame = 0

Query: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60
           MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR
Sbjct: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60

Query: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120
           IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN
Sbjct: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120

Query: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180
           PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG
Sbjct: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180

Query: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240
           ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240

Query: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300
           CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG
Sbjct: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300

Query: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360
           GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD
Sbjct: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360

Query: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP 420
           GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP
Sbjct: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP 420

Query: 421 FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ 480
           FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ
Sbjct: 421 FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ 480

Query: 481 GLATLFSPCRN 491
           GLATLFSPCRN
Sbjct: 481 GLATLFSPCRN 491

BLAST of MELO3C007311.jh1 vs. ExPASy TrEMBL
Match: A0A1S3B0H4 (sphingosine kinase 1 OS=Cucumis melo OX=3656 GN=LOC103484546 PE=4 SV=1)

HSP 1 Score: 1001 bits (2587), Expect = 0.0
Identity = 491/491 (100.00%), Postives = 491/491 (100.00%), Query Frame = 0

Query: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60
           MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR
Sbjct: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60

Query: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120
           IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN
Sbjct: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120

Query: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180
           PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG
Sbjct: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180

Query: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240
           ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240

Query: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300
           CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG
Sbjct: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300

Query: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360
           GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD
Sbjct: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360

Query: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP 420
           GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP
Sbjct: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP 420

Query: 421 FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ 480
           FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ
Sbjct: 421 FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ 480

Query: 481 GLATLFSPCRN 491
           GLATLFSPCRN
Sbjct: 481 GLATLFSPCRN 491

BLAST of MELO3C007311.jh1 vs. ExPASy TrEMBL
Match: A0A0A0KK35 (DAGKc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G517070 PE=4 SV=1)

HSP 1 Score: 969 bits (2506), Expect = 0.0
Identity = 475/491 (96.74%), Postives = 481/491 (97.96%), Query Frame = 0

Query: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60
           MDQS DSD ILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLS+DKEVLGFSMDGPKIR
Sbjct: 1   MDQSGDSDFILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSVDKEVLGFSMDGPKIR 60

Query: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120
           IKALVED GGLRCFG+SGALVRKEFVFQPLSEESRALWCLKLRECIDL+GRPKKLFVLVN
Sbjct: 61  IKALVEDHGGLRCFGSSGALVRKEFVFQPLSEESRALWCLKLRECIDLIGRPKKLFVLVN 120

Query: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180
           PFGGKG GSKIYRDEVKPILEDAEI+VTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG
Sbjct: 121 PFGGKGTGSKIYRDEVKPILEDAEIDVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180

Query: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240
           ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTA NATLAIVRGHK
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTACNATLAIVRGHK 240

Query: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300
           CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG
Sbjct: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300

Query: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360
           GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDG PIS QRHGYEGPNINLKDLEWRKFD
Sbjct: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGEPISIQRHGYEGPNINLKDLEWRKFD 360

Query: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP 420
           GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDC KLSLLSLMTELNNGKHVKSP
Sbjct: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCSKLSLLSLMTELNNGKHVKSP 420

Query: 421 FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ 480
           FVTYIKVKAF+LKPGTRV+EPSKEGIIDADGEILALGKGTLTT QKTLMNYDEL ISVHQ
Sbjct: 421 FVTYIKVKAFILKPGTRVKEPSKEGIIDADGEILALGKGTLTTSQKTLMNYDELLISVHQ 480

Query: 481 GLATLFSPCRN 491
           GLATLFSPCRN
Sbjct: 481 GLATLFSPCRN 491

BLAST of MELO3C007311.jh1 vs. ExPASy TrEMBL
Match: A0A5D3CP30 (Sphingosine kinase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007220 PE=4 SV=1)

HSP 1 Score: 904 bits (2336), Expect = 0.0
Identity = 456/497 (91.75%), Postives = 466/497 (93.76%), Query Frame = 0

Query: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60
           MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR
Sbjct: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60

Query: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120
           IKALVEDRGG RCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN
Sbjct: 61  IKALVEDRGGFRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120

Query: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180
           PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG
Sbjct: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180

Query: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240
           ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240

Query: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300
           CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG
Sbjct: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300

Query: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360
           GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD
Sbjct: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360

Query: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGF---LDLII---IRDCPKLSLLSLMTELNNG 420
           GPFISVWLHNVPWGAENTLAAPDAK    +   L +II   +++ P  +  S++ E  + 
Sbjct: 361 GPFISVWLHNVPWGAENTLAAPDAKDPPIWMPRLKVIIEPPVKENPIPNDSSIVFE-KSS 420

Query: 421 KHVKSPFVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDEL 480
                P     +VKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDEL
Sbjct: 421 LESNDPN----RVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDEL 480

Query: 481 QISVHQGLATLFSPCRN 491
           QISVHQGLATLFSPCRN
Sbjct: 481 QISVHQGLATLFSPCRN 492

BLAST of MELO3C007311.jh1 vs. ExPASy TrEMBL
Match: A0A6J1IMH9 (sphingosine kinase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478387 PE=4 SV=1)

HSP 1 Score: 892 bits (2306), Expect = 0.0
Identity = 430/491 (87.58%), Postives = 460/491 (93.69%), Query Frame = 0

Query: 1   MDQSEDSDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIR 60
           MDQS DS  ILNHRVLLPDG+VTPMILT DG L+W E+ QR LS+DKEVLGFS DGP IR
Sbjct: 1   MDQSGDSKPILNHRVLLPDGSVTPMILTADGCLRWLEEGQRSLSVDKEVLGFSTDGPNIR 60

Query: 61  IKALVEDRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVN 120
           IKALVED GGL CFG+SG LVRKEFVFQPLSEESRALWCLKLREC+DLLGRPK+LFV VN
Sbjct: 61  IKALVEDLGGLLCFGSSGGLVRKEFVFQPLSEESRALWCLKLRECVDLLGRPKRLFVFVN 120

Query: 121 PFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDG 180
           PFGG+ AG KIY D+VKPIL+DAEI VTLQETKYQRHAEEVAYSLD T YDGIVCVSGDG
Sbjct: 121 PFGGRRAGYKIYLDDVKPILDDAEIVVTLQETKYQRHAEEVAYSLDLTEYDGIVCVSGDG 180

Query: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHK 240
           ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGM KSLLHSIGDPCTA NATLAI+RGHK
Sbjct: 181 ILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMAKSLLHSIGDPCTACNATLAIIRGHK 240

Query: 241 CSLDVATISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRG 300
           CSLDVATISQGE KHF+VLMLAWGL+ADIDIESEKYRWMGSARLD YALQRIISLRHY+G
Sbjct: 241 CSLDVATISQGEVKHFSVLMLAWGLIADIDIESEKYRWMGSARLDFYALQRIISLRHYKG 300

Query: 301 GVSFVPAPGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFD 360
           GVSFVPAPGFEDYGEPTRYDYETASVV+VDKSDG   + +RHGYEGPNINLKDL WRKFD
Sbjct: 301 GVSFVPAPGFEDYGEPTRYDYETASVVDVDKSDGETDNIRRHGYEGPNINLKDLNWRKFD 360

Query: 361 GPFISVWLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSP 420
           GPFISVWLHNVPWGAENT+AAPDAKMSDGFLDLIIIRDCPKLSLL+LMTELNNG+HVKSP
Sbjct: 361 GPFISVWLHNVPWGAENTMAAPDAKMSDGFLDLIIIRDCPKLSLLALMTELNNGRHVKSP 420

Query: 421 FVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQ 480
           FVTYIKVKAF+L PGTRVEEP++EGIIDADGE+LALG+ T+T+GQKTLMNYD+LQIS+HQ
Sbjct: 421 FVTYIKVKAFILTPGTRVEEPNREGIIDADGEVLALGRETITSGQKTLMNYDKLQISMHQ 480

Query: 481 GLATLFSPCRN 491
           GLATLFSPCR+
Sbjct: 481 GLATLFSPCRD 491

BLAST of MELO3C007311.jh1 vs. TAIR 10
Match: AT4G21540.1 (sphingosine kinase 1 )

HSP 1 Score: 612.5 bits (1578), Expect = 3.1e-175
Identity = 292/482 (60.58%), Postives = 374/482 (77.59%), Query Frame = 0

Query: 7   SDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIRIKALVE 66
           S +I++ RVL+ +G VTP+ LT +G L+ +E  +R+ ++ KE+L F ++G K+R+K LVE
Sbjct: 14  SPAIISDRVLV-NGVVTPLTLTAEGELRSTESGRRKSTLAKEILSFVVEGNKVRVKTLVE 73

Query: 67  DRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVNPFGGKG 126
             GG+ C G++G   R +FVF+PLS+ESR LW  K  + +  LGRPKKL V VNPFGGK 
Sbjct: 74  KGGGICCRGSAGDYARNDFVFEPLSDESRKLWSDKFHQHLVSLGRPKKLLVFVNPFGGKK 133

Query: 127 AGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDGILVEVI 186
              KI+++EVKP+ EDA I + +QETKYQ HA+E+  S+D + YDGIVCVSGDGILVEV+
Sbjct: 134 TARKIFQEEVKPLFEDANIQLEIQETKYQLHAKEIVRSMDVSKYDGIVCVSGDGILVEVV 193

Query: 187 NGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHKCSLDVA 246
           NGLL R+DW  AIK P+G+VPAG+GNGM+KSLL  +G PC+A +AT++I+RG   SLDVA
Sbjct: 194 NGLLEREDWKTAIKLPIGMVPAGSGNGMIKSLLEPVGLPCSATSATISIIRGRTRSLDVA 253

Query: 247 TISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRGGVSFVP 306
           TISQG  K F+VLMLAWGLVADIDIESEK+RWMGSAR DIY LQRII LR Y G + FVP
Sbjct: 254 TISQGTTKFFSVLMLAWGLVADIDIESEKFRWMGSARFDIYGLQRIICLRQYHGRILFVP 313

Query: 307 APGFEDYGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFDGPFISV 366
           APGFE YG+         +   +DK      S +   Y+GP+  L++L+WR+  GPF+SV
Sbjct: 314 APGFESYGQ--------RASCSIDKEPSG--SDKTLVYQGPDSKLENLDWREMKGPFVSV 373

Query: 367 WLHNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSPFVTYIK 426
           WLHNVPWGAENTLAAPDAK SDGFLDLI+++DCPKL+LLSLMT+L++G HV+SP+ +Y+K
Sbjct: 374 WLHNVPWGAENTLAAPDAKFSDGFLDLIVMKDCPKLALLSLMTKLSDGTHVQSPYASYLK 433

Query: 427 VKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQGLATLF 486
           VKAFVL+PG R++EP KEGIID+DGE+LA G+ +    QK LM+YD+LQISV QGLATLF
Sbjct: 434 VKAFVLEPGARIDEPDKEGIIDSDGEVLARGRKSYKCDQKALMSYDKLQISVDQGLATLF 484

Query: 487 SP 489
           SP
Sbjct: 494 SP 484

BLAST of MELO3C007311.jh1 vs. TAIR 10
Match: AT4G21534.1 (Diacylglycerol kinase family protein )

HSP 1 Score: 588.2 bits (1515), Expect = 6.2e-168
Identity = 278/476 (58.40%), Postives = 365/476 (76.68%), Query Frame = 0

Query: 15  VLLPDGTVTPMILTTDGWLQWSEKSQRR-LSIDKEVLGFSMDGPKIRIKALVE-DRGGLR 74
           ++  DG +  + LT DG L+W+E   R+ LS+ K+VLGF ++G +IR+KA+VE + GG+ 
Sbjct: 16  IVFIDGELAMLTLTADGELRWTEYGLRQYLSMKKDVLGFIVEGKQIRVKAVVEKEAGGIC 75

Query: 75  CFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVNPFGGKGAGSKIY 134
           C    G  VRK+FVF+PL +++   WC KLR+ +D LGRPK+L V VNPFGGK +  +I+
Sbjct: 76  CGQFGGDFVRKDFVFEPLIDQNG--WCYKLRQYLDSLGRPKRLLVFVNPFGGKKSAREIF 135

Query: 135 RDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDGILVEVINGLLRR 194
             EVKP+ EDA++ + +QETKYQ HA+E   S+D + YDGIVCVSGDGILVEV+NGLL R
Sbjct: 136 VKEVKPLFEDADVQLEIQETKYQLHAKEFVKSMDVSKYDGIVCVSGDGILVEVVNGLLER 195

Query: 195 DDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHKCSLDVATISQGE 254
            DW +A+K P+G+VPAGTGNGM+KSLL ++G  C A +AT++I+RGHK S+DVATI+QG 
Sbjct: 196 ADWRNALKLPIGMVPAGTGNGMIKSLLDTVGLRCCANSATISIIRGHKRSVDVATIAQGN 255

Query: 255 AKHFTVLMLAWGLVADIDIESEKYRWMGSARLDIYALQRIISLRHYRGGVSFVPAPGFED 314
            K F+VLMLAWGL+ADIDIESEK+RWMGSAR+D YALQRII LR Y G + F+PAPGFE 
Sbjct: 256 TKFFSVLMLAWGLIADIDIESEKFRWMGSARIDFYALQRIICLRRYNGRILFLPAPGFEG 315

Query: 315 YGEPTRYDYETASVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFDGPFISVWLHNVP 374
           YG+P      + S+ +        +S +  GY+GP    +DLEWR+  GPF+++WLHNVP
Sbjct: 316 YGQPA-----SCSLYQEPH-----VSDKEVGYQGPETKFEDLEWREMKGPFVTIWLHNVP 375

Query: 375 WGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSPFVTYIKVKAFVL 434
           WG+ENTL AP AK SDG+LDLI++++CPKL LLSLM + ++G HV+SP++ YIKVKAFVL
Sbjct: 376 WGSENTLTAPAAKFSDGYLDLIVLKNCPKLVLLSLMRQTSSGTHVESPYIVYIKVKAFVL 435

Query: 435 KPGTRVEEPSKEGIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQGLATLFSP 489
           +PG  V+EP KEGIID+DGE+LA GK T    QK LM+YD+LQ++V QGLATLFSP
Sbjct: 436 EPGALVDEPDKEGIIDSDGEVLARGKRTYKCDQKALMSYDKLQVTVDQGLATLFSP 479

BLAST of MELO3C007311.jh1 vs. TAIR 10
Match: AT4G21540.2 (sphingosine kinase 1 )

HSP 1 Score: 386.7 bits (992), Expect = 2.7e-107
Identity = 184/284 (64.79%), Postives = 227/284 (79.93%), Query Frame = 0

Query: 205 VVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHKCSLDVATISQGEAKHFTVLMLAWG 264
           +VPAG+GNGM+KSLL  +G PC+A +AT++I+RG   SLDVATISQG  K F+VLMLAWG
Sbjct: 1   MVPAGSGNGMIKSLLEPVGLPCSATSATISIIRGRTRSLDVATISQGTTKFFSVLMLAWG 60

Query: 265 LVADIDIESEKYRWMGSARLDIYALQRIISLRHYRGGVSFVPAPGFEDYGEPTRYDYETA 324
           LVADIDIESEK+RWMGSAR DIY LQRII LR Y G + FVPAPGFE YG+         
Sbjct: 61  LVADIDIESEKFRWMGSARFDIYGLQRIICLRQYHGRILFVPAPGFESYGQ--------R 120

Query: 325 SVVEVDKSDGVPISTQRHGYEGPNINLKDLEWRKFDGPFISVWLHNVPWGAENTLAAPDA 384
           +   +DK      S +   Y+GP+  L++L+WR+  GPF+SVWLHNVPWGAENTLAAPDA
Sbjct: 121 ASCSIDKEPSG--SDKTLVYQGPDSKLENLDWREMKGPFVSVWLHNVPWGAENTLAAPDA 180

Query: 385 KMSDGFLDLIIIRDCPKLSLLSLMTELNNGKHVKSPFVTYIKVKAFVLKPGTRVEEPSKE 444
           K SDGFLDLI+++DCPKL+LLSLMT+L++G HV+SP+ +Y+KVKAFVL+PG R++EP KE
Sbjct: 181 KFSDGFLDLIVMKDCPKLALLSLMTKLSDGTHVQSPYASYLKVKAFVLEPGARIDEPDKE 240

Query: 445 GIIDADGEILALGKGTLTTGQKTLMNYDELQISVHQGLATLFSP 489
           GIID+DGE+LA G+ +    QK LM+YD+LQISV QGLATLFSP
Sbjct: 241 GIIDSDGEVLARGRKSYKCDQKALMSYDKLQISVDQGLATLFSP 274

BLAST of MELO3C007311.jh1 vs. TAIR 10
Match: AT4G21540.3 (sphingosine kinase 1 )

HSP 1 Score: 346.7 bits (888), Expect = 3.1e-95
Identity = 164/276 (59.42%), Postives = 215/276 (77.90%), Query Frame = 0

Query: 7   SDSILNHRVLLPDGTVTPMILTTDGWLQWSEKSQRRLSIDKEVLGFSMDGPKIRIKALVE 66
           S +I++ RVL+ +G VTP+ LT +G L+ +E  +R+ ++ KE+L F ++G K+R+K LVE
Sbjct: 14  SPAIISDRVLV-NGVVTPLTLTAEGELRSTESGRRKSTLAKEILSFVVEGNKVRVKTLVE 73

Query: 67  DRGGLRCFGNSGALVRKEFVFQPLSEESRALWCLKLRECIDLLGRPKKLFVLVNPFGGKG 126
             GG+ C G++G   R +FVF+PLS+ESR LW  K  + +  LGRPKKL V VNPFGGK 
Sbjct: 74  KGGGICCRGSAGDYARNDFVFEPLSDESRKLWSDKFHQHLVSLGRPKKLLVFVNPFGGKK 133

Query: 127 AGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFTNYDGIVCVSGDGILVEVI 186
              KI+++EVKP+ EDA I + +QETKYQ HA+E+  S+D + YDGIVCVSGDGILVEV+
Sbjct: 134 TARKIFQEEVKPLFEDANIQLEIQETKYQLHAKEIVRSMDVSKYDGIVCVSGDGILVEVV 193

Query: 187 NGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNATLAIVRGHKCSLDVA 246
           NGLL R+DW  AIK P+G+VPAG+GNGM+KSLL  +G PC+A +AT++I+RG   SLDVA
Sbjct: 194 NGLLEREDWKTAIKLPIGMVPAGSGNGMIKSLLEPVGLPCSATSATISIIRGRTRSLDVA 253

Query: 247 TISQGEAKHFTVLMLAWGLVADIDIESEKYRWMGSA 283
           TISQG  K F+VLMLAWGLVADIDIESEK+RWMGS+
Sbjct: 254 TISQGTTKFFSVLMLAWGLVADIDIESEKFRWMGSS 288

BLAST of MELO3C007311.jh1 vs. TAIR 10
Match: AT5G23450.1 (long-chain base (LCB) kinase 1 )

HSP 1 Score: 112.1 bits (279), Expect = 1.3e-24
Identity = 68/198 (34.34%), Postives = 111/198 (56.06%), Query Frame = 0

Query: 114 KLFVLVNPFGGKGAGSKIYRDEVKPILEDAEINVTLQETKYQRHAEEVAYSLDFT-NYDG 173
           K+ V++NP  G G   K++ + V+PI + A I + + +T    HA E+A ++D     DG
Sbjct: 249 KMLVILNPRSGHGRSIKVFHNVVEPIFKLAGIKMEVVKTTKAGHARELASTVDINLCSDG 308

Query: 174 IVCVSGDGILVEVINGLLRRDDWVDAIKTPLGVVPAGTGNGMVKSLLHSIGDPCTARNAT 233
           I+CV GDGI+ EV+NGLL R +  + +  P+G+VPAG+ N +V ++L  + DP    +A 
Sbjct: 309 IICVGGDGIINEVLNGLLTRSNPKEGVSIPIGIVPAGSDNSLVWTVL-GVRDPI---SAA 368

Query: 234 LAIVRGHKCSLDVATIS--QGEAKHFTVLMLAWGLVADIDIESEKY-RWMGSARLDIYAL 293
           L+IV+G   + DV  +        HF + +  +G V+D+   SEKY +  G  R  +   
Sbjct: 369 LSIVKGGLTATDVFAVEWIHTGIIHFGMTVSYYGFVSDVLELSEKYQKRFGPLRYFVAGF 428

Query: 294 QRIISLRHYRGGVSFVPA 308
            + + L  Y   V ++PA
Sbjct: 429 LKFMCLPKYSYEVEYLPA 442

HSP 2 Score: 57.4 bits (137), Expect = 3.8e-08
Identity = 31/111 (27.93%), Postives = 56/111 (50.45%), Query Frame = 0

Query: 355 EWRKFDGPFISVWL--HNVPWGAENTLAAPDAKMSDGFLDLIIIRDCPKLSLLSLMTELN 414
           +W    G F+ + +  H       + + AP+++  DG +D++++  C +L LL     L 
Sbjct: 641 KWVSRKGHFLGIMVCNHACRTVQSSQVVAPNSEHDDGTMDMLLVHGCGRLRLLRFFILLQ 700

Query: 415 NGKHVKSPFVTYIKVKAFVLKPGTRVEEPSKEGIIDADGEILALGKGTLTT 464
            G+H+  P+V  +KVK+  +K G    +    GI   DGE+ AL    ++T
Sbjct: 701 TGRHLSLPYVECVKVKSVKIKAGKNTHDSC--GI---DGELFALHGEVIST 746