MELO3C006613.jh1 (gene) Melon (Harukei-3) v1.41

BLAST of MELO3C006613.jh1 vs. NCBI nr
Match: XP_008439375.1 (PREDICTED: aspartic proteinase CDR1-like [Cucumis melo])

HSP 1 Score: 896 bits (2316), Expect = 0.0
Identity = 438/440 (99.55%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRL 60
           MILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRL
Sbjct: 1   MILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRL 60

Query: 61  SYLYYINKLSENTLENDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS 120
           SYLYYINKLSENTL+NDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS
Sbjct: 61  SYLYYINKLSENTLDNDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS 120

Query: 121 NCNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDN 180
           NCNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDN
Sbjct: 121 NCNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDN 180

Query: 181 KATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQL 240
           KATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQL
Sbjct: 181 KATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQL 240

Query: 241 GIKKFSYCLVPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHF 300
           GIKKFSYCLVPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHF
Sbjct: 241 GIKKFSYCLVPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHF 300

Query: 301 DGVFDVYDVRDGWIIDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELA 360
           DGVFDVYDVRDGWIIDTGITYSSLETDAFDSLLAKFL LKNFPQRKNDPKDRFELCFELA
Sbjct: 301 DGVFDVYDVRDGWIIDTGITYSSLETDAFDSLLAKFLALKNFPQRKNDPKDRFELCFELA 360

Query: 361 NANDLESFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYH 420
           NANDLESFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYH
Sbjct: 361 NANDLESFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYH 420

Query: 421 VGYDLEAQVISFAPVDCADS 440
           VGYDLEAQVISFAPVDCADS
Sbjct: 421 VGYDLEAQVISFAPVDCADS 440

BLAST of MELO3C006613.jh1 vs. NCBI nr
Match: KAA0049446.1 (aspartic proteinase CDR1-like [Cucumis melo var. makuwa] >TYK16125.1 aspartic proteinase CDR1-like [Cucumis melo var. makuwa])

HSP 1 Score: 872 bits (2253), Expect = 0.0
Identity = 424/426 (99.53%), Postives = 425/426 (99.77%), Query Frame = 0

Query: 15  MHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTL 74
           MHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTL
Sbjct: 1   MHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTL 60

Query: 75  ENDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPT 134
           +NDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPT
Sbjct: 61  DNDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPT 120

Query: 135 TKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGF 194
           TKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGF
Sbjct: 121 TKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGF 180

Query: 195 DTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNS 254
           DTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNS
Sbjct: 181 DTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNS 240

Query: 255 LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYDVRDGWI 314
           LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYDVRDGWI
Sbjct: 241 LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYDVRDGWI 300

Query: 315 IDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELANANDLESFPDATVH 374
           IDTGITYSSLETDAFDSLLAKFL LKNFPQRKNDPKDRFELCFELANANDLESFPDATVH
Sbjct: 301 IDTGITYSSLETDAFDSLLAKFLALKNFPQRKNDPKDRFELCFELANANDLESFPDATVH 360

Query: 375 FDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAP 434
           FDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAP
Sbjct: 361 FDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAP 420

Query: 435 VDCADS 440
           VDCADS
Sbjct: 421 VDCADS 426

BLAST of MELO3C006613.jh1 vs. NCBI nr
Match: XP_004134471.3 (aspartic proteinase CDR1 [Cucumis sativus] >KAE8650377.1 hypothetical protein Csa_011170 [Cucumis sativus])

HSP 1 Score: 862 bits (2227), Expect = 3.42e-315
Identity = 420/439 (95.67%), Postives = 429/439 (97.72%), Query Frame = 0

Query: 2   ILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLS 61
           IL+LLFTI ESKGMHMVSNEVGFTARLIHHDSPLSPFYNH MT TARIEATVHRSRSRL+
Sbjct: 11  ILFLLFTIFESKGMHMVSNEVGFTARLIHHDSPLSPFYNHTMTDTARIEATVHRSRSRLN 70

Query: 62  YLYYINKLSENTLENDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSN 121
           YLYYINKLSEN L+NDVSLSPTLVNEGGEYLMSFNIGNP SQVMGFLDTSNGLIWVQCSN
Sbjct: 71  YLYYINKLSENALDNDVSLSPTLVNEGGEYLMSFNIGNPSSQVMGFLDTSNGLIWVQCSN 130

Query: 122 CNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNK 181
           CNSQCEPEKRG TTKFLSSKSFTYEMEPCGSNFCNSLTGF+TCNSSDKWCKYRLVYGDNK
Sbjct: 131 CNSQCEPEKRGLTTKFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYGDNK 190

Query: 182 ATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLG 241
           ATSGILSSDSFGFDTSDG LVDVGFLNFGCSEAPLTGDEQSYTG VGLNQTPLSLISQLG
Sbjct: 191 ATSGILSSDSFGFDTSDGMLVDVGFLNFGCSEAPLTGDEQSYTGNVGLNQTPLSLISQLG 250

Query: 242 IKKFSYCLVPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFD 301
           IKKFSYCLVPFN+LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFD
Sbjct: 251 IKKFSYCLVPFNNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFD 310

Query: 302 GVFDVYDVRDGWIIDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELAN 361
           GVFDVY+VRDGWIIDTGITYSSLETDAFDSLLAKFLTLK+FPQRK+DPK+RFELCFEL N
Sbjct: 311 GVFDVYEVRDGWIIDTGITYSSLETDAFDSLLAKFLTLKDFPQRKDDPKERFELCFELQN 370

Query: 362 ANDLESFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHV 421
           ANDLESFPD TVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHV
Sbjct: 371 ANDLESFPDVTVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHV 430

Query: 422 GYDLEAQVISFAPVDCADS 440
           GYDLEAQVISFAPVDCADS
Sbjct: 431 GYDLEAQVISFAPVDCADS 449

BLAST of MELO3C006613.jh1 vs. NCBI nr
Match: XP_023528351.1 (aspartic proteinase CDR1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 746 bits (1927), Expect = 1.66e-269
Identity = 369/440 (83.86%), Postives = 398/440 (90.45%), Query Frame = 0

Query: 1   MILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRL 60
           M+ +L  TILES   HMV  EVGFTARLIH DSP+SPFY+H MT TA+IEATVHRSRSRL
Sbjct: 10  MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRL 69

Query: 61  SYLYYINKLSENTLENDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS 120
           +YLYY N LS+NTL+ND+SLSPTLV+EGGEYLMSFNIGNPPSQVMGF DTSNGLIWVQCS
Sbjct: 70  NYLYY-NMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCS 129

Query: 121 NCNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDN 180
           +CNSQCEPEK GP TKFL SKSFTYEMEPCGSN CNSLTGF+TCNSSD+WCKYRLVY DN
Sbjct: 130 DCNSQCEPEK-GPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDN 189

Query: 181 KATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQL 240
             TSG LSSDSF FDT+DGK VDVG+LNFGCSEAPLTG  QSY G VGLNQTPLSLISQL
Sbjct: 190 SETSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQL 249

Query: 241 GIKKFSYCLVPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHF 300
           GIKKFSYCLVPFN LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGIS+G D+P+ 
Sbjct: 250 GIKKFSYCLVPFN-LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNL 309

Query: 301 DGVFDVYDVRDGWIIDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELA 360
           DGVFDVYDVRDGWIID+G TYSSLETDAFD LLAKF+TL +  Q+K DP++RFELCF  A
Sbjct: 310 DGVFDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCF-AA 369

Query: 361 NANDLESFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYH 420
           NAND+E+FPD TVH DGA+LILNVESTFVKIEDDGI CLALLRSGSPVSILGNFQLQNYH
Sbjct: 370 NANDMETFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYH 429

Query: 421 VGYDLEAQVISFAPVDCADS 440
           VGYDLEAQV+SFAPV+CADS
Sbjct: 430 VGYDLEAQVVSFAPVNCADS 445

BLAST of MELO3C006613.jh1 vs. NCBI nr
Match: KAG6582237.1 (Aspartic proteinase CDR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 740 bits (1911), Expect = 3.25e-267
Identity = 368/440 (83.64%), Postives = 391/440 (88.86%), Query Frame = 0

Query: 1   MILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRL 60
           M+ +L  TILES   HMV  EVGFTARLIH DSPLSPFY+H MT TARIEATVHRSRSRL
Sbjct: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPLSPFYDHVMTNTARIEATVHRSRSRL 60

Query: 61  SYLYYINKLSENTLENDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS 120
           +YLYY N LS  TL+ND+SLSPTLV+EGGEYLMSFNIGNP SQVMGF DTSNGLIWVQCS
Sbjct: 61  NYLYY-NMLSRKTLDNDLSLSPTLVHEGGEYLMSFNIGNPSSQVMGFADTSNGLIWVQCS 120

Query: 121 NCNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDN 180
           +CNS CEPEK GP TKFL SKSFTYEMEPCGSN CNSLTGF+TCNSSD+WCKYRLVY DN
Sbjct: 121 DCNSHCEPEK-GPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDN 180

Query: 181 KATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQL 240
             TSG LSSDSF FDT+DGK VDVG+LNFGCSEAPL G  QSY G VGLNQTPLSLISQL
Sbjct: 181 SETSGTLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLIGGMQSYMGSVGLNQTPLSLISQL 240

Query: 241 GIKKFSYCLVPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHF 300
           GIKKFSYCLVPFN LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGIS+G D+P+ 
Sbjct: 241 GIKKFSYCLVPFN-LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGTDDPNL 300

Query: 301 DGVFDVYDVRDGWIIDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELA 360
           DGVFDVYDVRDGWIID+G TYSSLETDAFD LLAKF TL    Q+K DP++RFELCF  A
Sbjct: 301 DGVFDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFNTLPELQQKKEDPRNRFELCF-AA 360

Query: 361 NANDLESFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYH 420
           NAND+E+FPD TVH DGA+LILNVESTFVKIEDDGI CLALLRSGSPVSILGNFQLQNYH
Sbjct: 361 NANDMETFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYH 420

Query: 421 VGYDLEAQVISFAPVDCADS 440
           VGYDLEAQV+SFAPVDCADS
Sbjct: 421 VGYDLEAQVVSFAPVDCADS 436

BLAST of MELO3C006613.jh1 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.8e-59
Identity = 153/430 (35.58%), Postives = 226/430 (52.56%), Query Frame = 0

Query: 21  EVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTLENDVSL 80
           ++GFTA LIH DSP SPFYN   T + R+   +HRS +R+   ++  K  +NT +  + L
Sbjct: 28  KLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRV--FHFTEK--DNTPQPQIDL 87

Query: 81  SPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS---NCNSQCEPEKRGPTTKF 140
           +    +  GEYLM+ +IG PP  +M   DT + L+W QC+   +C +Q +P        F
Sbjct: 88  T----SNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDP-------LF 147

Query: 141 LSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFDTS 200
               S TY+   C S+ C +L    +C+++D  C Y L YGDN  T G ++ D+    +S
Sbjct: 148 DPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSS 207

Query: 201 DGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIK---KFSYCLVPFNS 260
           D + + +  +  GC         +  +G VGL   P+SLI QLG     KFSYCLVP  S
Sbjct: 208 DTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFSYCLVPLTS 267

Query: 261 -LGSTSKMYFGSLPVTSGG---QTPLLYPNSDA--YYVKVLGISIGNDEPHFDGVFDVYD 320
               TSK+ FG+  + SG     TPL+   S    YY+ +  IS+G+ +  + G  D   
Sbjct: 268 KKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSG-SDSES 327

Query: 321 VRDGWIIDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELANANDLESF 380
                IID+G T + L T+ F S L   +      ++K DP+    LC+  +   DL+  
Sbjct: 328 SEGNIIIDSGTTLTLLPTE-FYSELEDAVASSIDAEKKQDPQSGLSLCY--SATGDLK-V 387

Query: 381 PDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQ 439
           P  T+HFDGAD+ L+  + FV++ +D + C A  R     SI GN    N+ VGYD  ++
Sbjct: 388 PVITMHFDGADVKLDSSNAFVQVSED-LVCFA-FRGSPSFSIYGNVAQMNFLVGYDTVSK 435

BLAST of MELO3C006613.jh1 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 5.2e-54
Identity = 155/465 (33.33%), Postives = 230/465 (49.46%), Query Frame = 0

Query: 1   MILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRL 60
           +  +L F++  S   H       F+  LIH DSPLSP YN  +T T R+ A   RS SR 
Sbjct: 7   LCFFLFFSVTLSSSGH----PKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRS 66

Query: 61  SYLYYINKLSENTLENDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS 120
               + ++LS+  L++       L+   GE+ MS  IG PP +V    DT + L WVQC 
Sbjct: 67  R--RFNHQLSQTDLQSG------LIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCK 126

Query: 121 NCNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGF-KTCNSSDKWCKYRLVYGD 180
            C  QC  E  GP   F   KS TY+ EPC S  C +L+   + C+ S+  CKYR  YGD
Sbjct: 127 PC-QQCYKE-NGPI--FDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGD 186

Query: 181 NKATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQ 240
              + G +++++   D++ G  V      FGC        +++ +G +GL    LSLISQ
Sbjct: 187 QSFSKGDVATETVSIDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQ 246

Query: 241 LG---IKKFSYCL--------------VPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSD 300
           LG    KKFSYCL              +  NS+ S+     G +      + PL Y    
Sbjct: 247 LGSSISKKFSYCLSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTY---- 306

Query: 301 AYYVKVLGISIGNDE---------PHFDGVFDVYDVRDGWIIDTGITYSSLETDAFDSLL 360
            YY+ +  IS+G  +         P+ DG+    +     IID+G T + LE   FD   
Sbjct: 307 -YYLTLEAISVGKKKIPYTGSSYNPNDDGILS--ETSGNIIIDSGTTLTLLEAGFFDKFS 366

Query: 361 AKFLTLKNFPQRKNDPKDRFELCFELANANDLESFPDATVHFDGADLILNVESTFVKIED 420
           +         +R +DP+     CF+  +A      P+ TVHF GAD+ L+  + FVK+ +
Sbjct: 367 SAVEESVTGAKRVSDPQGLLSHCFKSGSAE--IGLPEITVHFTGADVRLSPINAFVKLSE 426

Query: 421 DGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAPVDCA 439
           D + CL+++ + + V+I GNF   ++ VGYDLE + +SF  +DC+
Sbjct: 427 D-MVCLSMVPT-TEVAIYGNFAQMDFLVGYDLETRTVSFQHMDCS 444

BLAST of MELO3C006613.jh1 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 6.8e-46
Identity = 135/426 (31.69%), Postives = 197/426 (46.24%), Query Frame = 0

Query: 23  GFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTLENDVSLSP 82
           GF   L H DS         +T    +E  + R   RL  L       E  L     +  
Sbjct: 40  GFQIMLEHVDS------GKNLTKFQLLERAIERGSRRLQRL-------EAMLNGPSGVET 99

Query: 83  TLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPTTKFLSSKS 142
           ++    GEYLM+ +IG P       +DT + LIW QC  C +QC  +    T  F    S
Sbjct: 100 SVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPC-TQCFNQS---TPIFNPQGS 159

Query: 143 FTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFDTSDGKLV 202
            ++   PC S  C +L+   TC  S+ +C+Y   YGD   T G + +++  F +     V
Sbjct: 160 SSFSTLPCSSQLCQALSS-PTC--SNNFCQYTYGYGDGSETQGSMGTETLTFGS-----V 219

Query: 203 DVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNSLGSTSKMY 262
            +  + FGC E      + +  G VG+ + PLSL SQL + KFSYC+ P  S  + S + 
Sbjct: 220 SIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGS-STPSNLL 279

Query: 263 FGSL--PVTSGGQTPLLYPNSDA---YYVKVLGISIGNDEPHFDG---VFDVYDVRDGWI 322
            GSL   VT+G     L  +S     YY+ + G+S+G+     D      +  +   G I
Sbjct: 280 LGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGII 339

Query: 323 IDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELANANDLESFPDATVH 382
           ID+G T +    +A+ S+  +F++  N P   N     F+LCF+  +       P   +H
Sbjct: 340 IDSGTTLTYFVNNAYQSVRQEFISQINLPV-VNGSSSGFDLCFQTPSDPSNLQIPTFVMH 399

Query: 383 FDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAP 441
           FDG DL L  E+ F+    +G+ CLA+  S   +SI GN Q QN  V YD    V+SFA 
Sbjct: 400 FDGGDLELPSENYFIS-PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFAS 437

BLAST of MELO3C006613.jh1 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 9.2e-43
Identity = 119/360 (33.06%), Postives = 177/360 (49.17%), Query Frame = 0

Query: 89  GEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPTTKFLSSKSFTYEME 148
           GEYLM+  IG P S     +DT + LIW QC  C +QC  +   PT  F    S ++   
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPC-TQCFSQ---PTPIFNPQDSSSFSTL 153

Query: 149 PCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFDTSDGKLVDVGFLN 208
           PC S +C  L   +TCN+++  C+Y   YGD   T G +++++F F+TS      V  + 
Sbjct: 154 PCESQYCQDLPS-ETCNNNE--CQYTYGYGDGSTTQGYMATETFTFETS-----SVPNIA 213

Query: 209 FGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNSLGSTSKMYFGSLP- 268
           FGC E      + +  G +G+   PLSL SQLG+ +FSYC+  + S  S S +  GS   
Sbjct: 214 FGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGS-SSPSTLALGSAAS 273

Query: 269 -VTSGG-QTPLLYP--NSDAYYVKVLGISIGNDEPHF-DGVFDVY-DVRDGWIIDTGITY 328
            V  G   T L++   N   YY+ + GI++G D        F +  D   G IID+G T 
Sbjct: 274 GVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTL 333

Query: 329 SSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELANANDLESFPDATVHFDGADLI 388
           + L  DA++++   F    N P   ++       CF+  +       P+ ++ FDG  L 
Sbjct: 334 TYLPQDAYNAVAQAFTDQINLP-TVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGGVLN 393

Query: 389 LNVESTFVKIEDDGIFCLALLRSGS-PVSILGNFQLQNYHVGYDLEAQVISFAPVDCADS 441
           L  ++  +    +G+ CLA+  S    +SI GN Q Q   V YDL+   +SF P  C  S
Sbjct: 394 LGEQNILIS-PAEGVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQCGAS 438

BLAST of MELO3C006613.jh1 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.9e-36
Identity = 120/366 (32.79%), Postives = 174/366 (47.54%), Query Frame = 0

Query: 89  GEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS---NCNSQCEPEKRGPTTKFLSSKSFTY 148
           GEY     +G P   V   LDT + ++W+QC+    C SQ +P        F   KS TY
Sbjct: 140 GEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDP-------IFDPRKSKTY 199

Query: 149 EMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFDTSDGKLVDVG 208
              PC S  C  L     CN+  K C Y++ YGD   T G  S+++  F  +  K V + 
Sbjct: 200 ATIPCSSPHCRRLDS-AGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVAL- 259

Query: 209 FLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIK---KFSYCLVPFNSLGSTSKMY 268
               GC      G      G +GL +  LS   Q G +   KFSYCLV  ++    S + 
Sbjct: 260 ----GCGH-DNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVV 319

Query: 269 FGSLPVTSGGQ-TPLL-YPNSDA-YYVKVLGISIGNDEPH--FDGVFDVYDVRDGW-IID 328
           FG+  V+   + TPLL  P  D  YYV +LGIS+G          +F +  + +G  IID
Sbjct: 320 FGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIID 379

Query: 329 TGITYSSLETDAF----DSLLAKFLTLKNFPQRKNDPKDRFELCFELANANDLESFPDAT 388
           +G + + L   A+    D+      TLK  P         F+ CF+L+N N+++  P   
Sbjct: 380 SGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPD-----FSLFDTCFDLSNMNEVK-VPTVV 439

Query: 389 VHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISF 439
           +HF GAD+ L   +  + ++ +G FC A   +   +SI+GN Q Q + V YDL +  + F
Sbjct: 440 LHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGF 485

BLAST of MELO3C006613.jh1 vs. ExPASy TrEMBL
Match: A0A1S3AZA6 (aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC103484191 PE=3 SV=1)

HSP 1 Score: 896 bits (2316), Expect = 0.0
Identity = 438/440 (99.55%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRL 60
           MILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRL
Sbjct: 1   MILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRL 60

Query: 61  SYLYYINKLSENTLENDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS 120
           SYLYYINKLSENTL+NDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS
Sbjct: 61  SYLYYINKLSENTLDNDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS 120

Query: 121 NCNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDN 180
           NCNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDN
Sbjct: 121 NCNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDN 180

Query: 181 KATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQL 240
           KATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQL
Sbjct: 181 KATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQL 240

Query: 241 GIKKFSYCLVPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHF 300
           GIKKFSYCLVPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHF
Sbjct: 241 GIKKFSYCLVPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHF 300

Query: 301 DGVFDVYDVRDGWIIDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELA 360
           DGVFDVYDVRDGWIIDTGITYSSLETDAFDSLLAKFL LKNFPQRKNDPKDRFELCFELA
Sbjct: 301 DGVFDVYDVRDGWIIDTGITYSSLETDAFDSLLAKFLALKNFPQRKNDPKDRFELCFELA 360

Query: 361 NANDLESFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYH 420
           NANDLESFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYH
Sbjct: 361 NANDLESFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYH 420

Query: 421 VGYDLEAQVISFAPVDCADS 440
           VGYDLEAQVISFAPVDCADS
Sbjct: 421 VGYDLEAQVISFAPVDCADS 440

BLAST of MELO3C006613.jh1 vs. ExPASy TrEMBL
Match: A0A5D3CXD4 (Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00490 PE=3 SV=1)

HSP 1 Score: 872 bits (2253), Expect = 0.0
Identity = 424/426 (99.53%), Postives = 425/426 (99.77%), Query Frame = 0

Query: 15  MHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTL 74
           MHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTL
Sbjct: 1   MHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTL 60

Query: 75  ENDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPT 134
           +NDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPT
Sbjct: 61  DNDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPT 120

Query: 135 TKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGF 194
           TKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGF
Sbjct: 121 TKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGF 180

Query: 195 DTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNS 254
           DTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNS
Sbjct: 181 DTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNS 240

Query: 255 LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYDVRDGWI 314
           LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYDVRDGWI
Sbjct: 241 LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYDVRDGWI 300

Query: 315 IDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELANANDLESFPDATVH 374
           IDTGITYSSLETDAFDSLLAKFL LKNFPQRKNDPKDRFELCFELANANDLESFPDATVH
Sbjct: 301 IDTGITYSSLETDAFDSLLAKFLALKNFPQRKNDPKDRFELCFELANANDLESFPDATVH 360

Query: 375 FDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAP 434
           FDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAP
Sbjct: 361 FDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAP 420

Query: 435 VDCADS 440
           VDCADS
Sbjct: 421 VDCADS 426

BLAST of MELO3C006613.jh1 vs. ExPASy TrEMBL
Match: A0A0A0L7U3 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G151520 PE=3 SV=1)

HSP 1 Score: 842 bits (2176), Expect = 4.53e-308
Identity = 409/426 (96.01%), Postives = 417/426 (97.89%), Query Frame = 0

Query: 15  MHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTL 74
           MHMVSNEVGFTARLIHHDSPLSPFYNH MT TARIEATVHRSRSRL+YLYYINKLSEN L
Sbjct: 1   MHMVSNEVGFTARLIHHDSPLSPFYNHTMTDTARIEATVHRSRSRLNYLYYINKLSENAL 60

Query: 75  ENDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPT 134
           +NDVSLSPTLVNEGGEYLMSFNIGNP SQVMGFLDTSNGLIWVQCSNCNSQCEPEKRG T
Sbjct: 61  DNDVSLSPTLVNEGGEYLMSFNIGNPSSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGLT 120

Query: 135 TKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGF 194
           TKFLSSKSFTYEMEPCGSNFCNSLTGF+TCNSSDKWCKYRLVYGDNKATSGILSSDSFGF
Sbjct: 121 TKFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYGDNKATSGILSSDSFGF 180

Query: 195 DTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNS 254
           DTSDG LVDVGFLNFGCSEAPLTGDEQSYTG VGLNQTPLSLISQLGIKKFSYCLVPFN+
Sbjct: 181 DTSDGMLVDVGFLNFGCSEAPLTGDEQSYTGNVGLNQTPLSLISQLGIKKFSYCLVPFNN 240

Query: 255 LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYDVRDGWI 314
           LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVY+VRDGWI
Sbjct: 241 LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYEVRDGWI 300

Query: 315 IDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELANANDLESFPDATVH 374
           IDTGITYSSLETDAFDSLLAKFLTLK+FPQRK+DPK+RFELCFEL NANDLESFPD TVH
Sbjct: 301 IDTGITYSSLETDAFDSLLAKFLTLKDFPQRKDDPKERFELCFELQNANDLESFPDVTVH 360

Query: 375 FDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAP 434
           FDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAP
Sbjct: 361 FDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAP 420

Query: 435 VDCADS 440
           VDCADS
Sbjct: 421 VDCADS 426

BLAST of MELO3C006613.jh1 vs. ExPASy TrEMBL
Match: A0A6J1GWK9 (aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111457819 PE=3 SV=1)

HSP 1 Score: 736 bits (1900), Expect = 1.04e-265
Identity = 366/440 (83.18%), Postives = 390/440 (88.64%), Query Frame = 0

Query: 1   MILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRL 60
           M+ +L  TILES   HMV  +VGFTARLIH DSPLSPFYNH MT TARIEATVHRSRSRL
Sbjct: 10  MMFFLSLTILESTARHMVPTDVGFTARLIHRDSPLSPFYNHVMTNTARIEATVHRSRSRL 69

Query: 61  SYLYYINKLSENTLENDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS 120
           +YLYY N LS  TL+ND+SLSPTLV+EGGEYLMSFNIGNP SQVMGF DTSNGLIWVQCS
Sbjct: 70  NYLYY-NMLSRKTLDNDLSLSPTLVHEGGEYLMSFNIGNPSSQVMGFADTSNGLIWVQCS 129

Query: 121 NCNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDN 180
           +CNS C+ EK GP TK L SKSFTYEMEPCGSN CNSLTGF+TCNSSD+WCKYRLVY DN
Sbjct: 130 DCNSHCDAEK-GPFTKLLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDN 189

Query: 181 KATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQL 240
             TSG LSSDSF FDT+DGK VDVG+LNFGCSEAPLTG  QSY G VGLNQTPLSLISQL
Sbjct: 190 SETSGTLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQL 249

Query: 241 GIKKFSYCLVPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHF 300
           GIKKFSYCLVPFN LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGIS+G D+P+ 
Sbjct: 250 GIKKFSYCLVPFN-LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNL 309

Query: 301 DGVFDVYDVRDGWIIDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELA 360
           DGVFDVYDVRDGWIID+G TYSSLETDAFD LLAKF TL    Q+K DP++RFELCF  A
Sbjct: 310 DGVFDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFNTLPELQQKKEDPRNRFELCF-AA 369

Query: 361 NANDLESFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYH 420
           NAND+E+FPD TVH DGA+LILNVESTFVKIEDDGI CLALLRSGSPVSILGNFQLQNYH
Sbjct: 370 NANDMETFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYH 429

Query: 421 VGYDLEAQVISFAPVDCADS 440
           VGYDLEAQV+SFAPVDCADS
Sbjct: 430 VGYDLEAQVVSFAPVDCADS 445

BLAST of MELO3C006613.jh1 vs. ExPASy TrEMBL
Match: A0A6J1IXB1 (aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111479389 PE=3 SV=1)

HSP 1 Score: 734 bits (1896), Expect = 4.23e-265
Identity = 366/440 (83.18%), Postives = 396/440 (90.00%), Query Frame = 0

Query: 1   MILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRL 60
           M+ +L FTILES   HMV  EVGFTARLIH DSPLSPFY+H M+ TA IEAT+HRSRSRL
Sbjct: 10  MMFFLSFTILESTARHMVPTEVGFTARLIHRDSPLSPFYDHVMSYTAWIEATIHRSRSRL 69

Query: 61  SYLYYINKLSENTLENDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS 120
           +YLYY N LS++TL+ND+SLSPTLV+EGGEYLMSFNIGNPPSQVMGF DTSNGLIWVQCS
Sbjct: 70  NYLYY-NMLSKDTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCS 129

Query: 121 NCNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDN 180
           +CNSQCEPEK GP TKFL SKSFTYEMEPCGSN CNSLTGF+TCNSSD+ CKYRLVY DN
Sbjct: 130 DCNSQCEPEK-GPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRSCKYRLVYEDN 189

Query: 181 KATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQL 240
             TSG LSSDSF FDT+DGK VDVG+LNFGCSEAPLTG  QSY G VGLNQTPLSLISQL
Sbjct: 190 SETSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQL 249

Query: 241 GIKKFSYCLVPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHF 300
           GIKKFSYCLVPFN LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGIS+G D+P+ 
Sbjct: 250 GIKKFSYCLVPFN-LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNL 309

Query: 301 DGVFDVYDVRDGWIIDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELA 360
           +GVFDVYDVRDGWIID+G TYSSLETDAFD LLAKF+TL +  Q+K DP++RFELCF  A
Sbjct: 310 EGVFDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCF-AA 369

Query: 361 NANDLESFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYH 420
           NAND+E+FP  TVHFDGA+LILNVESTFVKIEDDGI CLALLRSGSPVSILGNFQLQN H
Sbjct: 370 NANDMETFPGVTVHFDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNCH 429

Query: 421 VGYDLEAQVISFAPVDCADS 440
           VGYDLEAQV+SFAPVDCADS
Sbjct: 430 VGYDLEAQVVSFAPVDCADS 445

BLAST of MELO3C006613.jh1 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 231.5 bits (589), Expect = 1.3e-60
Identity = 153/430 (35.58%), Postives = 226/430 (52.56%), Query Frame = 0

Query: 21  EVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTLENDVSL 80
           ++GFTA LIH DSP SPFYN   T + R+   +HRS +R+   ++  K  +NT +  + L
Sbjct: 28  KLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRV--FHFTEK--DNTPQPQIDL 87

Query: 81  SPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS---NCNSQCEPEKRGPTTKF 140
           +    +  GEYLM+ +IG PP  +M   DT + L+W QC+   +C +Q +P        F
Sbjct: 88  T----SNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDP-------LF 147

Query: 141 LSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFDTS 200
               S TY+   C S+ C +L    +C+++D  C Y L YGDN  T G ++ D+    +S
Sbjct: 148 DPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSS 207

Query: 201 DGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIK---KFSYCLVPFNS 260
           D + + +  +  GC         +  +G VGL   P+SLI QLG     KFSYCLVP  S
Sbjct: 208 DTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFSYCLVPLTS 267

Query: 261 -LGSTSKMYFGSLPVTSGG---QTPLLYPNSDA--YYVKVLGISIGNDEPHFDGVFDVYD 320
               TSK+ FG+  + SG     TPL+   S    YY+ +  IS+G+ +  + G  D   
Sbjct: 268 KKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSG-SDSES 327

Query: 321 VRDGWIIDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELANANDLESF 380
                IID+G T + L T+ F S L   +      ++K DP+    LC+  +   DL+  
Sbjct: 328 SEGNIIIDSGTTLTLLPTE-FYSELEDAVASSIDAEKKQDPQSGLSLCY--SATGDLK-V 387

Query: 381 PDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQ 439
           P  T+HFDGAD+ L+  + FV++ +D + C A  R     SI GN    N+ VGYD  ++
Sbjct: 388 PVITMHFDGADVKLDSSNAFVQVSED-LVCFA-FRGSPSFSIYGNVAQMNFLVGYDTVSK 435

BLAST of MELO3C006613.jh1 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 213.4 bits (542), Expect = 3.7e-55
Identity = 155/465 (33.33%), Postives = 230/465 (49.46%), Query Frame = 0

Query: 1   MILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRL 60
           +  +L F++  S   H       F+  LIH DSPLSP YN  +T T R+ A   RS SR 
Sbjct: 7   LCFFLFFSVTLSSSGH----PKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRS 66

Query: 61  SYLYYINKLSENTLENDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS 120
               + ++LS+  L++       L+   GE+ MS  IG PP +V    DT + L WVQC 
Sbjct: 67  R--RFNHQLSQTDLQSG------LIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCK 126

Query: 121 NCNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGF-KTCNSSDKWCKYRLVYGD 180
            C  QC  E  GP   F   KS TY+ EPC S  C +L+   + C+ S+  CKYR  YGD
Sbjct: 127 PC-QQCYKE-NGPI--FDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGD 186

Query: 181 NKATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQ 240
              + G +++++   D++ G  V      FGC        +++ +G +GL    LSLISQ
Sbjct: 187 QSFSKGDVATETVSIDSASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQ 246

Query: 241 LG---IKKFSYCL--------------VPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSD 300
           LG    KKFSYCL              +  NS+ S+     G +      + PL Y    
Sbjct: 247 LGSSISKKFSYCLSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTY---- 306

Query: 301 AYYVKVLGISIGNDE---------PHFDGVFDVYDVRDGWIIDTGITYSSLETDAFDSLL 360
            YY+ +  IS+G  +         P+ DG+    +     IID+G T + LE   FD   
Sbjct: 307 -YYLTLEAISVGKKKIPYTGSSYNPNDDGILS--ETSGNIIIDSGTTLTLLEAGFFDKFS 366

Query: 361 AKFLTLKNFPQRKNDPKDRFELCFELANANDLESFPDATVHFDGADLILNVESTFVKIED 420
           +         +R +DP+     CF+  +A      P+ TVHF GAD+ L+  + FVK+ +
Sbjct: 367 SAVEESVTGAKRVSDPQGLLSHCFKSGSAE--IGLPEITVHFTGADVRLSPINAFVKLSE 426

Query: 421 DGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAPVDCA 439
           D + CL+++ + + V+I GNF   ++ VGYDLE + +SF  +DC+
Sbjct: 427 D-MVCLSMVPT-TEVAIYGNFAQMDFLVGYDLETRTVSFQHMDCS 444

BLAST of MELO3C006613.jh1 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 205.3 bits (521), Expect = 1.0e-52
Identity = 147/439 (33.49%), Postives = 216/439 (49.20%), Query Frame = 0

Query: 19  SNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTLENDV 78
           +N    T  LIH DSP SP YN   T + R+ A   RS SR                   
Sbjct: 24  ANRENLTVELIHRDSPHSPLYNPHHTVSDRLNAAFLRSISR-----------SRRFTTKT 83

Query: 79  SLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPTTKFL 138
            L   L++ GGEY MS +IG PPS+V    DT + L WVQC  C  QC  +    +  F 
Sbjct: 84  DLQSGLISNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQCKPC-QQCYKQN---SPLFD 143

Query: 139 SSKSFTYEMEPCGSNFCNSLTGFKT-CNSSDKWCKYRLVYGDNKATSGILSSDSFGFDTS 198
             KS TY+ E C S  C +L+  +  C+ S   CKYR  YGDN  T G +++++   D+S
Sbjct: 144 KKKSSTYKTESCDSKTCQALSEHEEGCDESKDICKYRYSYGDNSFTKGDVATETISIDSS 203

Query: 199 DGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGI---KKFSYCLV-PFN 258
            G  V      FGC        E++ +G +GL   PLSL+SQLG    KKFSYCL     
Sbjct: 204 SGSSVSFPGTVFGCGYNNGGTFEETGSGIIGLGGGPLSLVSQLGSSIGKKFSYCLSHTAA 263

Query: 259 SLGSTSKMYFGSLPVTSGGQ-------TPLLYPNSDAYYVKVL-GISIGNDE-PHFDGVF 318
           +   TS +  G+  + S          TPL+  + + YY   L  +++G  + P+  G +
Sbjct: 264 TTNGTSVINLGTNSIPSNPSKDSATLTTPLIQKDPETYYFLTLEAVTVGKTKLPYTGGGY 323

Query: 319 DV---YDVRDG-WIIDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELA 378
            +      R G  IID+G T + L++  +D             +R +DP+     CF+  
Sbjct: 324 GLNGKSSKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGAKRVSDPQGLLTHCFK-- 383

Query: 379 NANDLE-SFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNY 438
            + D E   P  T+HF  AD+ L+  + FVK+ +D + CL+++ + + V+I GN    ++
Sbjct: 384 -SGDKEIGLPAITMHFTNADVKLSPINAFVKLNEDTV-CLSMIPT-TEVAIYGNMVQMDF 442

BLAST of MELO3C006613.jh1 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 196.8 bits (499), Expect = 3.6e-50
Identity = 142/431 (32.95%), Postives = 215/431 (49.88%), Query Frame = 0

Query: 23  GFTARLIHHDSPLSPFYNHAMTGTARIEATVHRS-RSRLSYLYYINKLSENTLENDVSLS 82
           GFT  LIH DSP SPFYN A T + R+   + RS RS L +         N   +  S  
Sbjct: 25  GFTIDLIHRDSPKSPFYNSAETSSQRMRNAIRRSARSTLQF--------SNDDASPNSPQ 84

Query: 83  PTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPTTKFLSSK 142
             + +  GEYLM+ +IG PP  ++   DT + LIW QC    + CE   +  +  F   +
Sbjct: 85  SFITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQC----NPCEDCYQQTSPLFDPKE 144

Query: 143 SFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFDTSDGKL 202
           S TY    C S+ C +L    +C++ +  C Y + YGDN  T G ++ D+    +S  + 
Sbjct: 145 SSTYRKVSCSSSQCRALED-ASCSTDENTCSYTITYGDNSYTKGDVAVDTVTMGSSGRRP 204

Query: 203 VDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIK---KFSYCLVPFNS-LGS 262
           V +  +  GC        + + +G +GL     SL+SQL      KFSYCLVPF S  G 
Sbjct: 205 VSLRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQLRKSINGKFSYCLVPFTSETGL 264

Query: 263 TSKMYFGSLPVTSGG---QTPLLYPNSDAYY-VKVLGISIGNDEPHFDGVFDVYDVRDG- 322
           TSK+ FG+  + SG     T ++  +   YY + +  IS+G+ +  F     ++   +G 
Sbjct: 265 TSKINFGTNGIVSGDGVVSTSMVKKDPATYYFLNLEAISVGSKKIQFTST--IFGTGEGN 324

Query: 323 WIIDTGITYSSLETDAF---DSLLAKFLTLKNFPQRKNDPKDRFELCFELANANDLESF- 382
            +ID+G T + L ++ +   +S++A   T+K   +R  DP     LC+      D  SF 
Sbjct: 325 IVIDSGTTLTLLPSNFYYELESVVAS--TIK--AERVQDPDGILSLCY-----RDSSSFK 384

Query: 383 -PDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEA 439
            PD TVHF G D+ L   +TFV + +D + C A   +   ++I GN    N+ VGYD  +
Sbjct: 385 VPDITVHFKGGDVKLGNLNTFVAVSED-VSCFA-FAANEQLTIFGNLAQMNFLVGYDTVS 429

BLAST of MELO3C006613.jh1 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 193.0 bits (489), Expect = 5.2e-49
Identity = 141/432 (32.64%), Postives = 210/432 (48.61%), Query Frame = 0

Query: 23  GFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTLENDVSLSP 82
           GF   L H DS         +T   +I+  ++R   RL+ L  +  L+  +  +D +   
Sbjct: 44  GFRLSLRHVDS------GKNLTKIQKIQRGINRGFHRLNRLGAVAVLAVASKPDDTNNIK 103

Query: 83  TLVNEG-GEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPTTKFLSSK 142
              + G GE+LM  +IGNP  +    +DT + LIW QC  C ++C  +   PT  F   K
Sbjct: 104 APTHGGSGEFLMELSIGNPAVKYSAIVDTGSDLIWTQCKPC-TECFDQ---PTPIFDPEK 163

Query: 143 SFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFDTSDGKL 202
           S +Y    C S  CN+L     CN     C+Y   YGD  +T G+L++++F F+  +   
Sbjct: 164 SSSYSKVGCSSGLCNALPR-SNCNEDKDACEYLYTYGDYSSTRGLLATETFTFEDEN--- 223

Query: 203 VDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNSLGSTSKM 262
             +  + FGC            +G VGL + PLSLISQL   KFSYCL       ++S +
Sbjct: 224 -SISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQLKETKFSYCLTSIEDSEASSSL 283

Query: 263 YFGSLP---VTSGG--------QTPLLYPNSDA---YYVKVLGISIGNDEPHFD-GVFDV 322
           + GSL    V   G        +T  L  N D    YY+++ GI++G      +   F++
Sbjct: 284 FIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQGITVGAKRLSVEKSTFEL 343

Query: 323 -YDVRDGWIIDTGITYSSLETDAFDSLLAKFLTLKNFPQRKNDPKDRFELCFELANANDL 382
             D   G IID+G T + LE  AF  L  +F +  + P   +      +LCF+L +A   
Sbjct: 344 AEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLPV-DDSGSTGLDLCFKLPDAAKN 403

Query: 383 ESFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDL 438
            + P    HF GADL L  E+  V     G+ CLA + S + +SI GN Q QN++V +DL
Sbjct: 404 IAVPKMIFHFKGADLELPGENYMVADSSTGVLCLA-MGSSNGMSIFGNVQQQNFNVLHDL 458