MELO3C006236 (gene) Melon (DHL92) v4

BLAST of MELO3C006236 vs. NCBI nr
Match: XP_008438233.1 (PREDICTED: E3 ubiquitin-protein ligase PUB23-like [Cucumis melo])

HSP 1 Score: 815.5 bits (2105), Expect = 2.2e-232
Identity = 415/415 (100.00%), Postives = 415/415 (100.00%), Query Frame = 0

Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60
           MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120
           FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120

Query: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180
           GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240
           EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF
Sbjct: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300
           PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 416
           LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE
Sbjct: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

BLAST of MELO3C006236 vs. NCBI nr
Match: KAA0049079.1 (E3 ubiquitin-protein ligase PUB23-like [Cucumis melo var. makuwa] >TYK17484.1 E3 ubiquitin-protein ligase PUB23-like [Cucumis melo var. makuwa])

HSP 1 Score: 814.3 bits (2102), Expect = 4.8e-232
Identity = 414/415 (99.76%), Postives = 415/415 (100.00%), Query Frame = 0

Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60
           MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120
           FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRS+IEMND
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSMIEMND 120

Query: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180
           GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240
           EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF
Sbjct: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300
           PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 416
           LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE
Sbjct: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

BLAST of MELO3C006236 vs. NCBI nr
Match: XP_004134357.1 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN56649.1 hypothetical protein Csa_010227 [Cucumis sativus])

HSP 1 Score: 797.0 bits (2057), Expect = 7.9e-227
Identity = 402/415 (96.87%), Postives = 409/415 (98.55%), Query Frame = 0

Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60
           MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGA+D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60

Query: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120
           FVITPNL LKRLILAWKINDSA TPATALEEVSSLLATVESSPFKVSSLRKLRS++EMND
Sbjct: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120

Query: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180
           GMKSEF+RLSGIGIVVNVVIQILIDCSDFSTL+TCEEALGVLCQFPISNEDKLFDEMYKR
Sbjct: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240
           EELMKSI VV+QRGSAEGRFYAVKILRNIAKNTNYNWGS+MEEQ IDFFKPLLELVSDEF
Sbjct: 181 EELMKSIAVVVQRGSAEGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300
           PTKASSCALDVMI ILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 PTKASSCALDVMIEILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 416
           LLMILHMDS GVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE
Sbjct: 361 LLMILHMDSTGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

BLAST of MELO3C006236 vs. NCBI nr
Match: XP_038902365.1 (E3 ubiquitin-protein ligase PUB23-like [Benincasa hispida])

HSP 1 Score: 768.8 bits (1984), Expect = 2.3e-218
Identity = 388/415 (93.49%), Postives = 404/415 (97.35%), Query Frame = 0

Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60
           M+SDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTL+AG +D
Sbjct: 1   MESDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLSAGDED 60

Query: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120
           FVITPNLTLKRLILAWKI DS+S+PATALEEV SLLATVESSPFKVSSLRKLRS+IEM+D
Sbjct: 61  FVITPNLTLKRLILAWKITDSSSSPATALEEVRSLLATVESSPFKVSSLRKLRSMIEMSD 120

Query: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180
           GMKSEF+R +GIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPIS+EDKLFDEM KR
Sbjct: 121 GMKSEFIRSNGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISDEDKLFDEMSKR 180

Query: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240
           EELMKS+ +VLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQ IDFFKPLLELVSDEF
Sbjct: 181 EELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQGIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300
            TKASSCALDVMI IL+SSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 ATKASSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVS VATK+GVKILLWISSFHPRERVV+EMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSNVATKMGVKILLWISSFHPRERVVEEMMACGAVKK 360

Query: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 416
           LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGN WRRSPCFPSELKNYLKFVND+
Sbjct: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNSWRRSPCFPSELKNYLKFVNDD 415

BLAST of MELO3C006236 vs. NCBI nr
Match: XP_023528267.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 730.3 bits (1884), Expect = 9.1e-207
Identity = 366/415 (88.19%), Postives = 392/415 (94.46%), Query Frame = 0

Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYNK+TCPATMQT+A G +D
Sbjct: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72

Query: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120
           FV+TPNLTLKR+ILAWKIN+S+S+PATA+++V SLLATVESSPFKVSSLRKLRS+IEM D
Sbjct: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132

Query: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180
            +KSEF+RL+GIGIVVNVV+QILIDCSDFSTLE CEEALGVL QFPISNEDKLFDEM KR
Sbjct: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDKLFDEMSKR 192

Query: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240
           EELMKS+ +VLQRGSAEGRFYAVKILRNIAKNTNYNWG VMEEQ ID FKPLLELVSDEF
Sbjct: 193 EELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEF 252

Query: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300
           PTK+SSCALDVMI IL+SSKRSRVKAIEAGAVWILIELLPES RSKCERMLQILQSLCEC
Sbjct: 253 PTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCERMLQILQSLCEC 312

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVS VATKIGVKILLWISSFHP+ERVV+EMMACGAVKK
Sbjct: 313 AEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILLWISSFHPKERVVEEMMACGAVKK 372

Query: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 416
           LLMILHM   GVG GGRSSTKEKVIKILKMHG+ WRRSPCFP ELK YLK VND+
Sbjct: 373 LLMILHMAGGGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKYYLKLVNDD 427

BLAST of MELO3C006236 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.9e-54
Identity = 144/416 (34.62%), Postives = 214/416 (51.44%), Query Frame = 0

Query: 4   DFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADDFVI 63
           + PP F CPIS+E+M+DPV VSTG+TYDR SIEKWLF   K +CP T Q +     D  +
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDIT----DADL 70

Query: 64  TPNLTLKRLILAW-KINDS-------ASTPATALEEVSSLLA-TVESSPFKVSSLRKLRS 123
           TPN TL+RLI +W  +N S          P     E+  L+  +  S   +V  L++LR 
Sbjct: 71  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 130

Query: 124 IIEMNDGMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNED-KL 183
           I+  N   K           + N+V       +D       +EAL +L     S    K 
Sbjct: 131 IVSENATNKRCLEAAGVPEFLANIV------SNDSENGSLTDEALNLLYHLETSETVLKN 190

Query: 184 FDEMYKREELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLL 243
                K   ++KS+T ++QRG  E R YA  +L+NI +  +      ++ +    F  ++
Sbjct: 191 LLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEV---FTEVV 250

Query: 244 ELVSDEFPTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPE----SGRSKCER 303
           +++ D    KA+  A+ +++ I     R+R KA+EAG + ++IELL +    S R   E 
Sbjct: 251 QILDDRISQKATKAAMHILVNICPWG-RNRHKAVEAGVISVIIELLMDESFTSERRGPEM 310

Query: 304 MLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVV 363
            + +L  LC+CAEGR   + HG  IA V KKIL VS  A+   V++LL +  F     ++
Sbjct: 311 AMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALL 370

Query: 364 DEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSEL 406
            EM+  G V KL ++L      V  GG+  TKEK  ++LK+H   W+ SPC P  +
Sbjct: 371 HEMLQLGVVAKLCLVLQ-----VSCGGK--TKEKAKELLKLHARVWKDSPCLPKNM 405

BLAST of MELO3C006236 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.9e-50
Identity = 144/439 (32.80%), Postives = 220/439 (50.11%), Query Frame = 0

Query: 4   DFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADDFVI 63
           + P  F CPIS+++M+DPV VSTG+TYDR+SIEKWLF+  K +CP T Q +     +  +
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVIT----ETDL 65

Query: 64  TPNLTLKRLILAW-KINDS-------ASTPATALEEVSSLLATVESSPF-KVSSLRKLRS 123
           TPN TL+RLI +W  +N S          P     E+  L+    SS   +V  L++LR 
Sbjct: 66  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 125

Query: 124 IIEMNDGMKSEFVRLSGIGIVVNVVIQI---------------LIDCSDFSTLET----- 183
           I+  N   K           + N+V                  L D    + LE      
Sbjct: 126 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 185

Query: 184 ---CEEALGVLCQFPISNED-KLFDEMYKREELMKSITVVLQRGSAEGRFYAVKILRNIA 243
               +EAL VL     S    K      K   L+K++T ++QRG  E R YA  +L+ + 
Sbjct: 186 RSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLL 245

Query: 244 KNTNYNWGSVMEEQAIDFFKPLLELVSDEFPTKASSCALDVMIGILSSSKRSRVKAIEAG 303
           +  +     ++E    + F  +++++ D+   KA+  A+ +++ I     R+R KA+E G
Sbjct: 246 EVADPMQIILLER---ELFGEVIQILHDQISHKATRSAMQILV-ITCPWGRNRHKAVEGG 305

Query: 304 AVWILIELLPE----SGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSY 363
            + ++IELL +    S R   E  + +L  LC+CAEGR   + HG  IA V+KKIL VS 
Sbjct: 306 TISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQ 365

Query: 364 VATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVIK 406
           + ++  V++LL +  F     ++ EM+  G V KL ++L + S G      + TKEK  +
Sbjct: 366 ITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQV-SCG------NKTKEKAKE 425

BLAST of MELO3C006236 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 7.1e-37
Identity = 138/449 (30.73%), Postives = 218/449 (48.55%), Query Frame = 0

Query: 4   DFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADDFVI 63
           + P  F CPIS+E+M+DPVT  +G+TYDR++I KWL      +CP T Q L   +D   +
Sbjct: 9   EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSD---L 68

Query: 64  TPNLTLKRLILAWKINDSA------STP------ATALEEVSSLLATVESSPFKVSSLRK 123
           TPN  L+RLI  W + +        STP         +EE+ +L    + +  +  +L+K
Sbjct: 69  TPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQK 128

Query: 124 LRSIIEMNDGMKSEFVRLSGI--GIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPI-S 183
           L  ++ M DG     +   G+   +++ VV     D      ++  +E+L +L    I S
Sbjct: 129 L-EVLAM-DGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPS 188

Query: 184 NEDKLFDEMYKREELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVME------ 243
           N+ K    + + + +M+S+T VL +     + Y + +LRN+   T Y    ++E      
Sbjct: 189 NDAKTI--LMENDRVMESLTWVLHQEDFLSKAYTIVLLRNL---TEYTSSHIVERLNPEI 248

Query: 244 -EQAIDFFKPLLELVSDEFPT----------------------------KASSCALDVMI 303
            +  I F K ++  V+   PT                            +A + AL +++
Sbjct: 249 FKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILL 308

Query: 304 GILSSSKRSRVKAIEAGAVWILIEL-LPESGRSK-CERMLQILQSLCECAEGRLALVEHG 363
              SS  R+R   ++ GAV  LIEL +  +G  +  E ML +L  LC CA GR  ++ H 
Sbjct: 309 E-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHR 368

Query: 364 MGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSVG 401
            GIA VTK++L VS  A    + IL  +S F P   VV+EM+  G V+KL  +L MD   
Sbjct: 369 GGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDC-- 428

BLAST of MELO3C006236 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 9.0e-32
Identity = 120/408 (29.41%), Postives = 193/408 (47.30%), Query Frame = 0

Query: 6   PPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADDFVITP 65
           P  FRCPIS+ELM+DPVTV TG TYDR SIE W+   N  TCP T   L+    DF + P
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS----DFTLIP 74

Query: 66  NLTLKRLILAWKINDSAS------TP---------ATALEEVSSLLATVESSPFKVSSLR 125
           N TL+RLI  W + + ++      TP            L + S++  T  S   + ++LR
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 134

Query: 126 KLRSIIEMNDGMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNE 185
           +LR     +D  +      +   I++ ++       S+ ++ E   E+L +L   PI+ E
Sbjct: 135 RLRGFARDSDKNRVLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPIT-E 194

Query: 186 DKLFDEMYKREELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYN--WGSVMEEQAIDF 245
              F  +      ++ +T +L   S E R  A  ++  ++  T      GS+   +++  
Sbjct: 195 PNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV-- 254

Query: 246 FKPLLELVSDEFPTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIE-LLPESGRSKC 305
           F+ +L+L+ +   ++ +       +  L S K +R  AI AGA  ILI+ L  +  R   
Sbjct: 255 FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDT 314

Query: 306 ERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRER 365
           ER L  ++ LC   EG  A  EH + +  + K IL VS  AT+     LL + +   R R
Sbjct: 315 ERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEERWR 374

Query: 366 VVDEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKW 396
             +E    G V +LL+++  +            K+K  K+LK+  + W
Sbjct: 375 --EEAAGAGVVVQLLLMVQSECT-------ERAKKKAQKLLKLLRDSW 400

BLAST of MELO3C006236 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 7.4e-26
Identity = 113/406 (27.83%), Postives = 188/406 (46.31%), Query Frame = 0

Query: 6   PPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADDFVITP 65
           P  FRCPIS++LM DPVT+STG TYDR SI+ W+   N  TCP T   L+    DF + P
Sbjct: 15  PYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALS----DFTLIP 74

Query: 66  NLTLKRLILAWKINDSAS------TP---------ATALEEVSSLLATVESSPFKVSSLR 125
           N TL+RLI  W + + ++      TP          + L + S++  T  S   + +++R
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIR 134

Query: 126 KLRSIIEMNDGMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNE 185
           +LR +   ++  +      +   I+V ++    I+ +  S+ E   E+L +L    ++  
Sbjct: 135 RLRGLARDSEKNRVLIAGHNAREILVRILFAD-IETTSLSS-ELVSESLALLVLLHMTET 194

Query: 186 DKLFDEMYKREELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFK 245
           +   + +      +  +T +L   S E R  A  ++  +          ++   +   F+
Sbjct: 195 E--CEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFE 254

Query: 246 PLLELVSDEFPTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIE-LLPESGRSKCER 305
            +L+L+ +   ++ +       I  L   K++R  AI AGA  ILI+ L  +  R   ER
Sbjct: 255 GVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDTER 314

Query: 306 MLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVV 365
            L  ++ LC   EG  A  EH + +  + K IL VS  AT+     LL + +    ER  
Sbjct: 315 GLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCT--AEERCR 374

Query: 366 DEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKW 396
           DE  A G V +LL+++  D            K K   +LK+  + W
Sbjct: 375 DEAAAAGLVTQLLLLVQSDCT-------ERAKRKAQMLLKLLRDSW 402

BLAST of MELO3C006236 vs. ExPASy TrEMBL
Match: A0A1S3AWJ2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103483401 PE=4 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.0e-232
Identity = 415/415 (100.00%), Postives = 415/415 (100.00%), Query Frame = 0

Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60
           MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120
           FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120

Query: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180
           GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240
           EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF
Sbjct: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300
           PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 416
           LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE
Sbjct: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

BLAST of MELO3C006236 vs. ExPASy TrEMBL
Match: A0A5D3D2G2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003490 PE=4 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 2.3e-232
Identity = 414/415 (99.76%), Postives = 415/415 (100.00%), Query Frame = 0

Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60
           MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60

Query: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120
           FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRS+IEMND
Sbjct: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSMIEMND 120

Query: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180
           GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR
Sbjct: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240
           EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF
Sbjct: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300
           PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 416
           LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE
Sbjct: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

BLAST of MELO3C006236 vs. ExPASy TrEMBL
Match: A0A0A0L6J2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G127120 PE=4 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 3.8e-227
Identity = 402/415 (96.87%), Postives = 409/415 (98.55%), Query Frame = 0

Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60
           MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGA+D
Sbjct: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGAED 60

Query: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120
           FVITPNL LKRLILAWKINDSA TPATALEEVSSLLATVESSPFKVSSLRKLRS++EMND
Sbjct: 61  FVITPNLNLKRLILAWKINDSALTPATALEEVSSLLATVESSPFKVSSLRKLRSMVEMND 120

Query: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180
           GMKSEF+RLSGIGIVVNVVIQILIDCSDFSTL+TCEEALGVLCQFPISNEDKLFDEMYKR
Sbjct: 121 GMKSEFIRLSGIGIVVNVVIQILIDCSDFSTLDTCEEALGVLCQFPISNEDKLFDEMYKR 180

Query: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240
           EELMKSI VV+QRGSAEGRFYAVKILRNIAKNTNYNWGS+MEEQ IDFFKPLLELVSDEF
Sbjct: 181 EELMKSIAVVVQRGSAEGRFYAVKILRNIAKNTNYNWGSIMEEQGIDFFKPLLELVSDEF 240

Query: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300
           PTKASSCALDVMI ILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC
Sbjct: 241 PTKASSCALDVMIEILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK
Sbjct: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360

Query: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 416
           LLMILHMDS GVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE
Sbjct: 361 LLMILHMDSTGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 415

BLAST of MELO3C006236 vs. ExPASy TrEMBL
Match: A0A6J1IYW3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111479714 PE=4 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 4.4e-207
Identity = 365/415 (87.95%), Postives = 392/415 (94.46%), Query Frame = 0

Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYNK+TCPATMQT+A G +D
Sbjct: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72

Query: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120
           FV+TPNLTLKR+ILAWKIN+S+S+PATA+++V SLLATVESSPFKVSSLRKLRS+IEM D
Sbjct: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132

Query: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180
            +KSEF+RL+GIGIVVNVV+QILIDCSDFSTLE CEEALGVL QFPISNED+LFDEM KR
Sbjct: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDELFDEMSKR 192

Query: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240
           EELMKS+ +VLQRGSAEGRFYAVKILRNIAKNTNYNWG VMEEQ ID FKPLLELVSDEF
Sbjct: 193 EELMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEF 252

Query: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300
           PTK+SSCALDVMI IL+SSKRSRVKAIEAGAVWILIELLPES RSKCERMLQILQSLCEC
Sbjct: 253 PTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCERMLQILQSLCEC 312

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVS VATKIGVKIL WISSFHP+ERVV+EMMACGAVKK
Sbjct: 313 AEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILFWISSFHPKERVVEEMMACGAVKK 372

Query: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 416
           LLMILHM   GVG GGRSSTKEKVIKILKMHG+ WRRSPCFP ELKNYLK VND+
Sbjct: 373 LLMILHMACSGVGGGGRSSTKEKVIKILKMHGSSWRRSPCFPCELKNYLKLVNDD 427

BLAST of MELO3C006236 vs. ExPASy TrEMBL
Match: A0A6J1E9J6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432079 PE=4 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 2.2e-206
Identity = 365/415 (87.95%), Postives = 391/415 (94.22%), Query Frame = 0

Query: 1   MDSDFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADD 60
           MDSDFPPLFRCPISMELMEDPVT+STGVTY+RKSIEKWLFTYNK+TCPATMQT+A G +D
Sbjct: 13  MDSDFPPLFRCPISMELMEDPVTISTGVTYERKSIEKWLFTYNKRTCPATMQTVAVGEND 72

Query: 61  FVITPNLTLKRLILAWKINDSASTPATALEEVSSLLATVESSPFKVSSLRKLRSIIEMND 120
           FV+TPNLTLKR+ILAWKIN+S+S+PATA+++V SLLATVESSPFKVSSLRKLRS+IEM D
Sbjct: 73  FVMTPNLTLKRVILAWKINESSSSPATAIDQVQSLLATVESSPFKVSSLRKLRSMIEMRD 132

Query: 121 GMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNEDKLFDEMYKR 180
            +KSEF+RL+GIGIVVNVV+QILIDCSDFSTLE CEEALGVL QFPISNEDKLFDEM KR
Sbjct: 133 EVKSEFIRLNGIGIVVNVVVQILIDCSDFSTLEACEEALGVLSQFPISNEDKLFDEMSKR 192

Query: 181 EELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLLELVSDEF 240
           EE MKS+ +VLQRGSAEGRFYAVKILRNIAKNTNYNWG VMEEQ ID FKPLLELVSDEF
Sbjct: 193 EEWMKSVAIVLQRGSAEGRFYAVKILRNIAKNTNYNWGFVMEEQGIDLFKPLLELVSDEF 252

Query: 241 PTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPESGRSKCERMLQILQSLCEC 300
           PTK+SSCALDVMI IL+SSKRSRVKAIEAGAVWILIELLPES RSKCERMLQILQSLCEC
Sbjct: 253 PTKSSSCALDVMIEILASSKRSRVKAIEAGAVWILIELLPESSRSKCERMLQILQSLCEC 312

Query: 301 AEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKK 360
           AEGRLALVEHGMGIAAVTKKILNVS VATKIGVKILLWISSFHP+ERVV+EMMACGAVKK
Sbjct: 313 AEGRLALVEHGMGIAAVTKKILNVSNVATKIGVKILLWISSFHPKERVVEEMMACGAVKK 372

Query: 361 LLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSELKNYLKFVNDE 416
           LLMILHM S GVG GGRSSTKEKVIKILKMHG+ WRR PCFP ELK YLK VND+
Sbjct: 373 LLMILHMASGGVGGGGRSSTKEKVIKILKMHGSSWRRGPCFPCELKYYLKLVNDD 427

BLAST of MELO3C006236 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 214.2 bits (544), Expect = 2.0e-55
Identity = 144/416 (34.62%), Postives = 214/416 (51.44%), Query Frame = 0

Query: 4   DFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADDFVI 63
           + PP F CPIS+E+M+DPV VSTG+TYDR SIEKWLF   K +CP T Q +     D  +
Sbjct: 11  EIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDIT----DADL 70

Query: 64  TPNLTLKRLILAW-KINDS-------ASTPATALEEVSSLLA-TVESSPFKVSSLRKLRS 123
           TPN TL+RLI +W  +N S          P     E+  L+  +  S   +V  L++LR 
Sbjct: 71  TPNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQ 130

Query: 124 IIEMNDGMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNED-KL 183
           I+  N   K           + N+V       +D       +EAL +L     S    K 
Sbjct: 131 IVSENATNKRCLEAAGVPEFLANIV------SNDSENGSLTDEALNLLYHLETSETVLKN 190

Query: 184 FDEMYKREELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFKPLL 243
                K   ++KS+T ++QRG  E R YA  +L+NI +  +      ++ +    F  ++
Sbjct: 191 LLNNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEV---FTEVV 250

Query: 244 ELVSDEFPTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIELLPE----SGRSKCER 303
           +++ D    KA+  A+ +++ I     R+R KA+EAG + ++IELL +    S R   E 
Sbjct: 251 QILDDRISQKATKAAMHILVNICPWG-RNRHKAVEAGVISVIIELLMDESFTSERRGPEM 310

Query: 304 MLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVV 363
            + +L  LC+CAEGR   + HG  IA V KKIL VS  A+   V++LL +  F     ++
Sbjct: 311 AMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALL 370

Query: 364 DEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKWRRSPCFPSEL 406
            EM+  G V KL ++L      V  GG+  TKEK  ++LK+H   W+ SPC P  +
Sbjct: 371 HEMLQLGVVAKLCLVLQ-----VSCGGK--TKEKAKELLKLHARVWKDSPCLPKNM 405

BLAST of MELO3C006236 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 201.4 bits (511), Expect = 1.4e-51
Identity = 144/439 (32.80%), Postives = 220/439 (50.11%), Query Frame = 0

Query: 4   DFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADDFVI 63
           + P  F CPIS+++M+DPV VSTG+TYDR+SIEKWLF+  K +CP T Q +     +  +
Sbjct: 6   EIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVIT----ETDL 65

Query: 64  TPNLTLKRLILAW-KINDS-------ASTPATALEEVSSLLATVESSPF-KVSSLRKLRS 123
           TPN TL+RLI +W  +N S          P     E+  L+    SS   +V  L++LR 
Sbjct: 66  TPNHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQ 125

Query: 124 IIEMNDGMKSEFVRLSGIGIVVNVVIQI---------------LIDCSDFSTLET----- 183
           I+  N   K           + N+V                  L D    + LE      
Sbjct: 126 IVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSS 185

Query: 184 ---CEEALGVLCQFPISNED-KLFDEMYKREELMKSITVVLQRGSAEGRFYAVKILRNIA 243
               +EAL VL     S    K      K   L+K++T ++QRG  E R YA  +L+ + 
Sbjct: 186 RSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLL 245

Query: 244 KNTNYNWGSVMEEQAIDFFKPLLELVSDEFPTKASSCALDVMIGILSSSKRSRVKAIEAG 303
           +  +     ++E    + F  +++++ D+   KA+  A+ +++ I     R+R KA+E G
Sbjct: 246 EVADPMQIILLER---ELFGEVIQILHDQISHKATRSAMQILV-ITCPWGRNRHKAVEGG 305

Query: 304 AVWILIELLPE----SGRSKCERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSY 363
            + ++IELL +    S R   E  + +L  LC+CAEGR   + HG  IA V+KKIL VS 
Sbjct: 306 TISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQ 365

Query: 364 VATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVIK 406
           + ++  V++LL +  F     ++ EM+  G V KL ++L + S G      + TKEK  +
Sbjct: 366 ITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQV-SCG------NKTKEKAKE 425

BLAST of MELO3C006236 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 156.4 bits (394), Expect = 5.1e-38
Identity = 138/449 (30.73%), Postives = 218/449 (48.55%), Query Frame = 0

Query: 4   DFPPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADDFVI 63
           + P  F CPIS+E+M+DPVT  +G+TYDR++I KWL      +CP T Q L   +D   +
Sbjct: 23  EIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPLDSD---L 82

Query: 64  TPNLTLKRLILAWKINDSA------STP------ATALEEVSSLLATVESSPFKVSSLRK 123
           TPN  L+RLI  W + +        STP         +EE+ +L    + +  +  +L+K
Sbjct: 83  TPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQK 142

Query: 124 LRSIIEMNDGMKSEFVRLSGI--GIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPI-S 183
           L  ++ M DG     +   G+   +++ VV     D      ++  +E+L +L    I S
Sbjct: 143 L-EVLAM-DGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPS 202

Query: 184 NEDKLFDEMYKREELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVME------ 243
           N+ K    + + + +M+S+T VL +     + Y + +LRN+   T Y    ++E      
Sbjct: 203 NDAKTI--LMENDRVMESLTWVLHQEDFLSKAYTIVLLRNL---TEYTSSHIVERLNPEI 262

Query: 244 -EQAIDFFKPLLELVSDEFPT----------------------------KASSCALDVMI 303
            +  I F K ++  V+   PT                            +A + AL +++
Sbjct: 263 FKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILL 322

Query: 304 GILSSSKRSRVKAIEAGAVWILIEL-LPESGRSK-CERMLQILQSLCECAEGRLALVEHG 363
              SS  R+R   ++ GAV  LIEL +  +G  +  E ML +L  LC CA GR  ++ H 
Sbjct: 323 E-TSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHR 382

Query: 364 MGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVVDEMMACGAVKKLLMILHMDSVG 401
            GIA VTK++L VS  A    + IL  +S F P   VV+EM+  G V+KL  +L MD   
Sbjct: 383 GGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDC-- 442

BLAST of MELO3C006236 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 139.4 bits (350), Expect = 6.4e-33
Identity = 120/408 (29.41%), Postives = 193/408 (47.30%), Query Frame = 0

Query: 6   PPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADDFVITP 65
           P  FRCPIS+ELM+DPVTV TG TYDR SIE W+   N  TCP T   L+    DF + P
Sbjct: 15  PYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS----DFTLIP 74

Query: 66  NLTLKRLILAWKINDSAS------TP---------ATALEEVSSLLATVESSPFKVSSLR 125
           N TL+RLI  W + + ++      TP            L + S++  T  S   + ++LR
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 134

Query: 126 KLRSIIEMNDGMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNE 185
           +LR     +D  +      +   I++ ++       S+ ++ E   E+L +L   PI+ E
Sbjct: 135 RLRGFARDSDKNRVLIAAHNATEILIKILF------SETTSSELVSESLALLVMLPIT-E 194

Query: 186 DKLFDEMYKREELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYN--WGSVMEEQAIDF 245
              F  +      ++ +T +L   S E R  A  ++  ++  T      GS+   +++  
Sbjct: 195 PNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV-- 254

Query: 246 FKPLLELVSDEFPTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIE-LLPESGRSKC 305
           F+ +L+L+ +   ++ +       +  L S K +R  AI AGA  ILI+ L  +  R   
Sbjct: 255 FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDT 314

Query: 306 ERMLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRER 365
           ER L  ++ LC   EG  A  EH + +  + K IL VS  AT+     LL + +   R R
Sbjct: 315 ERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEERWR 374

Query: 366 VVDEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKW 396
             +E    G V +LL+++  +            K+K  K+LK+  + W
Sbjct: 375 --EEAAGAGVVVQLLLMVQSECT-------ERAKKKAQKLLKLLRDSW 400

BLAST of MELO3C006236 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 119.8 bits (299), Expect = 5.3e-27
Identity = 113/406 (27.83%), Postives = 188/406 (46.31%), Query Frame = 0

Query: 6   PPLFRCPISMELMEDPVTVSTGVTYDRKSIEKWLFTYNKKTCPATMQTLAAGADDFVITP 65
           P  FRCPIS++LM DPVT+STG TYDR SI+ W+   N  TCP T   L+    DF + P
Sbjct: 15  PYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGN-TTCPVTRVALS----DFTLIP 74

Query: 66  NLTLKRLILAWKINDSAS------TP---------ATALEEVSSLLATVESSPFKVSSLR 125
           N TL+RLI  W + + ++      TP          + L + S++  T  S   + +++R
Sbjct: 75  NHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIR 134

Query: 126 KLRSIIEMNDGMKSEFVRLSGIGIVVNVVIQILIDCSDFSTLETCEEALGVLCQFPISNE 185
           +LR +   ++  +      +   I+V ++    I+ +  S+ E   E+L +L    ++  
Sbjct: 135 RLRGLARDSEKNRVLIAGHNAREILVRILFAD-IETTSLSS-ELVSESLALLVLLHMTET 194

Query: 186 DKLFDEMYKREELMKSITVVLQRGSAEGRFYAVKILRNIAKNTNYNWGSVMEEQAIDFFK 245
           +   + +      +  +T +L   S E R  A  ++  +          ++   +   F+
Sbjct: 195 E--CEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFE 254

Query: 246 PLLELVSDEFPTKASSCALDVMIGILSSSKRSRVKAIEAGAVWILIE-LLPESGRSKCER 305
            +L+L+ +   ++ +       I  L   K++R  AI AGA  ILI+ L  +  R   ER
Sbjct: 255 GVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDTER 314

Query: 306 MLQILQSLCECAEGRLALVEHGMGIAAVTKKILNVSYVATKIGVKILLWISSFHPRERVV 365
            L  ++ LC   EG  A  EH + +  + K IL VS  AT+     LL + +    ER  
Sbjct: 315 GLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCT--AEERCR 374

Query: 366 DEMMACGAVKKLLMILHMDSVGVGDGGRSSTKEKVIKILKMHGNKW 396
           DE  A G V +LL+++  D            K K   +LK+  + W
Sbjct: 375 DEAAAAGLVTQLLLLVQSDCT-------ERAKRKAQMLLKLLRDSW 402