MELO3C005483 (gene) Melon (DHL92) v4

Overview
NameMELO3C005483
Typegene
OrganismCucumis melo cv. DHL92 (Melon (DHL92) v4)
DescriptionProtein NRT1/ PTR FAMILY 5.10-like
Locationchr09: 20623664 .. 20626856 (+)
RNA-Seq ExpressionMELO3C005483
SyntenyMELO3C005483
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTGAGTGAAGGGAAAGTGATTGGAGAAAGGGGTAGTTAACGGTATTATTGTGGAAGTTGAGGGAACTTATGGACTAAATAAATGAATCTAAAGTGAAGATTAGAAAGATATATTTTATTACTCGGAGGATCCGCGACGACTCAGTTCTGATCCATTTCTCTCTCCCGCCCCTTTTTTCGCCACCATCCCCCACCCTCCCGATTTCTTCTCCGACGACCAACTCTGGAATCTCCAAACTCACGCCCACGCTGCATTGTTCTTTTCTCCGGCGGATTTCTCTTCAGCTAGCTTCAAGGCGGCGGTGCTACTCAAGCTCACGACAGTGACGACAGAAACCCGCGCCTTTCACCCCTCAACTGGCAGTGACAAGGCTTTTCTTGACCGATTATAAACCGGACACCGCTTTTGCCCTCAATTCAGTTTGTACAGAGCTTGAAGAAGACAAAACGGCGAAATCGAACTTATTCGATTAAGAACCAAACTAAATTTGATGCTGTTTGCCGTCTTTTAGTAGATTCGTACTTCCAGCAGCAATCACGAGTCAATTTTGGTAAGATTTGGATACTGAAGTTGTGTTTAAACCTTTTCAATCTCGACTAGAGTAGGTTAGACGTTCTTTAACCTTTGGAAAGTTTCTGTATGCAGTTTTTTTTGACAATTTGTCATTTGTTAAGCCAAGAGATACCATAGAACTATACGTTTTTTGTTTTTCTCGTGATGGCTACCGAAAATTCAAGGTTTTGAGCAAATTATTGATTTCGGTTTGGCTTTTGCCAATAAAATTGGATGAATGGTCTGATAATGAGATTGATGCAATGATAGAAATTGGAGGAAATGATTTTGCTAATGCAATTATATGCGGCCTTTATCTCAGTTGGATATACAAAACATGGACTAAGGAGTGTTCAAAGTTCATTTGGTAAGATTCGTACTTGCAAGCGTTTGTAAGTAATAAGTGTAAAATGGAGGTTCCTCTATTGGATGAGACGGTGGAGGGTGCAGTTGATTACAATGGCAACCCAGTCTGTAGATCCAATTCCGGCGGCTGGAGATCTGCCTCCCTCATTATAGGTAAATGTAAATCATCATTAGCGTCTTCTTTTTCTTCTCTTTCCCTATGACCCTATCCAATTTATGTATCCTGTCCGATTGTTATAAGGAGTGGAGGTTGCTGAGAGATTTGCCTACTATGGAGTTTCCTCCAACTTGATTAACTTCTTGACGGACCAGCTTCAGCAGTCAACGGTCACGGCAGCTAAGAATGTCAATGCCTGGTCCGGTACCGCCTCCTTGCTACCTTTACTCGGCGCCTTTCTGGCTGATTCATTCCTAGGACGATACTGGACTATTGTTCTTTCCTCTGCCCTCTACATTCTGGTTCCTCCACTACTTTATTCTCTTGTTTTGTGTGCTTCTCCTTTTCCATTCCCATTTCGCAACTATAAAGTTTTACAAGATTATGCAAGTTTCTGGGTAGGGATATGATAATTTAGGATTATTTTTTCTTTTGTTGTTTCTGGAATTTAGGGCCTTGGGTTGTTGACCATGTCTGCATTGATATCTTCTCCAAGCACTTCTGCCTGCCAACAGACTGAAAAATCCTTACCATGTTCTCCCAATCTTTTCCAAGTTATTTTGTTTTTCTTCTCCTTATATTTGGTCGGATTGGCTCAAGGGGGACACAAGCCTTGCGTCCAAGCTTTTGGAGCCGACCAATTTGATGGACAACATCCAGAAGAGAGCATATCTAAAAGCTCTTTCTTCAATTGGTGGTATTTTGGTATCTCTTTGGCCACTTTTGCAACTGTTAATATCTTGAACTATGTGCAAGATAACCTCAGCTGGAGTCTTGGTTTTGGAATTCCTTGTATTGCTATGGTTTTAGCACTTGTAGTCTTCTTCCTTGGAACTAGGACGTACAGGTTCAGTAACAGAGGGGATGAGGAAAACCCATTTGTAAGAATTGGACGGGTGTTTATCACTGCAGTAAAAAATTGGCGAGTGAATTCTTCAGAAATGGCTCGTGAAGAGGAAATCCGTGGCCTTTTACCTCACCATAGCTCTAAACAGTTGAGGTAAATTAGATGTCTAAAACAAGGGTAACGTTGGCTTAGAAACAATTTTAATATGTATGTATTATTGTAACACTTTTCTTTACTGGATTCTATAATTTGGTAGGTTTCTCGATAAGGCTTTGATCGCACCTAATAGTTTGAAGGAAGACGGTCGTGCATGTAGCATCAGTGAAGTTGAGGAAGCAAAAGCAGTCCTGAGGCTTGTTCCGATATGGGTGACATGCTTAGCATTTGCTATTGTATTCTCTCAGTCATCAACTTTCTTTACAAAACAAGGAGTCACAATGGATAGATCAATAGCTTTTGGGTTTGAAGTACCGGCTGCTTCACTTCAATCTTTTATCAGTCTAGCCATTGTCATTTCCCTTATGATATATGATCGCATACTCATTCCGATTGCAAGAAATTTCACTGGGAAACCTTCTGGAATAACAACGCTACAAAGAATTGGATTTGGGATGCTACTATCTGCTATTTCCATGGTCATTGCTGCATTAGTTGAGATTAAAAGGCTTAAAACAGCTCAAGAATATGGTTTGGTTGATCTGCCTAAGGCGACTGTTCCTTTGAGTATATGGTGGCTGGTTCCTCAGTATGTTCTGTTTGGAGTTGCCGATGCTTTCACCATGGTTGGGTTGCAGGAGTTTTTTTATGACCAAGTTCCGAGTGGATTAAGAAGCATTGGTCTCTCCTTGTATTTGAGTATCTTTGGGATTGGCAACTTTTTAAGCAGCTTTCTCATCTCTGCCATTGAAAAATTAACTAGTGGGAATGGCAAACAGAGTTGGTTTGACAATAATCTCAACAAAGCTCATCTCGATTACTTCTACTGGTTACTTGTTGGACTTAGTGCTCTTGGGTTGGCTGCTTTCTTATTTTCTACCAGAACTTACATCTATAACAAGGGGAACACCATGTAAGTATAATTTGGTAGGGTGTATTTGGTCTGAGGAGTTAGAGAAATAGGAGTTGGGAAGTGAAAGTAGTAAGCTCCAATTTCGTTTGTTCTAAGGGAATTTGTTGGTCTCACTATCGAAAAACATCAATTTTGTATATTATCTACTATTTAAGTTGTGGGTGGAGAGTTCCCGAATCCCTAGATTTCAGGATTCCTTAG

mRNA sequence

ACTGAGTGAAGGGAAAGTGATTGGAGAAAGGGGTAGTTAACGGTATTATTGTGGAAGTTGAGGGAACTTATGGACTAAATAAATGAATCTAAAGTGAAGATTAGAAAGATATATTTTATTACTCGGAGGATCCGCGACGACTCAGTTCTGATCCATTTCTCTCTCCCGCCCCTTTTTTCGCCACCATCCCCCACCCTCCCGATTTCTTCTCCGACGACCAACTCTGGAATCTCCAAACTCACGCCCACGCTGCATTGTTCTTTTCTCCGGCGGATTTCTCTTCAGCTAGCTTCAAGGCGGCGGTGCTACTCAAGCTCACGACAGTGACGACAGAAACCCGCGCCTTTCACCCCTCAACTGGCAGTGACAAGGCTTTTCTTGACCGATTATAAACCGGACACCGCTTTTGCCCTCAATTCAGTTTGTACAGAGCTTGAAGAAGACAAAACGGCGAAATCGAACTTATTCGATTAAGAACCAAACTAAATTTGATGCTGTTTGCCGTCTTTTAGTAGATTCGTACTTCCAGCAGCAATCACGAGTCAATTTTGGTAAGATTTGGATACTGAAGTTGTGTTTAAACCTTTTCAATCTCGACTAGAGTAGTTGGATATACAAAACATGGACTAAGGAGTGTTCAAAGTTCATTTGGTAAGATTCGTACTTGCAAGCGTTTGTAAGTAATAAGTGTAAAATGGAGGTTCCTCTATTGGATGAGACGGTGGAGGGTGCAGTTGATTACAATGGCAACCCAGTCTGTAGATCCAATTCCGGCGGCTGGAGATCTGCCTCCCTCATTATAGGAGTGGAGGTTGCTGAGAGATTTGCCTACTATGGAGTTTCCTCCAACTTGATTAACTTCTTGACGGACCAGCTTCAGCAGTCAACGGTCACGGCAGCTAAGAATGTCAATGCCTGGTCCGGTACCGCCTCCTTGCTACCTTTACTCGGCGCCTTTCTGGCTGATTCATTCCTAGGACGATACTGGACTATTGTTCTTTCCTCTGCCCTCTACATTCTGGTTCCTCCACTACTTTATTCTCTTGTTTTGTGTGCTTCTCCTTTTCCATTCCCATTTCGCAACTATAAAGTTTTACAAGATTATGCAAGTTTCTGGGGCCTTGGGTTGTTGACCATGTCTGCATTGATATCTTCTCCAAGCACTTCTGCCTGCCAACAGACTGAAAAATCCTTACCATGTTCTCCCAATCTTTTCCAAGTTATTTTGTTTTTCTTCTCCTTATATTTGGTCGGATTGGCTCAAGGGGGACACAAGCCTTGCGTCCAAGCTTTTGGAGCCGACCAATTTGATGGACAACATCCAGAAGAGAGCATATCTAAAAGCTCTTTCTTCAATTGGTGGTATTTTGGTATCTCTTTGGCCACTTTTGCAACTGTTAATATCTTGAACTATGTGCAAGATAACCTCAGCTGGAGTCTTGGTTTTGGAATTCCTTGTATTGCTATGGTTTTAGCACTTGTAGTCTTCTTCCTTGGAACTAGGACGTACAGGTTCAGTAACAGAGGGGATGAGGAAAACCCATTTGTAAGAATTGGACGGGTGTTTATCACTGCAGTAAAAAATTGGCGAGTGAATTCTTCAGAAATGGCTCGTGAAGAGGAAATCCGTGGCCTTTTACCTCACCATAGCTCTAAACAGTTGAGGTTTCTCGATAAGGCTTTGATCGCACCTAATAGTTTGAAGGAAGACGGTCGTGCATGTAGCATCAGTGAAGTTGAGGAAGCAAAAGCAGTCCTGAGGCTTGTTCCGATATGGGTGACATGCTTAGCATTTGCTATTGTATTCTCTCAGTCATCAACTTTCTTTACAAAACAAGGAGTCACAATGGATAGATCAATAGCTTTTGGGTTTGAAGTACCGGCTGCTTCACTTCAATCTTTTATCAGTCTAGCCATTGTCATTTCCCTTATGATATATGATCGCATACTCATTCCGATTGCAAGAAATTTCACTGGGAAACCTTCTGGAATAACAACGCTACAAAGAATTGGATTTGGGATGCTACTATCTGCTATTTCCATGGTCATTGCTGCATTAGTTGAGATTAAAAGGCTTAAAACAGCTCAAGAATATGGTTTGGTTGATCTGCCTAAGGCGACTGTTCCTTTGAGTATATGGTGGCTGGTTCCTCAGTATGTTCTGTTTGGAGTTGCCGATGCTTTCACCATGGTTGGGTTGCAGGAGTTTTTTTATGACCAAGTTCCGAGTGGATTAAGAAGCATTGGTCTCTCCTTGTATTTGAGTATCTTTGGGATTGGCAACTTTTTAAGCAGCTTTCTCATCTCTGCCATTGAAAAATTAACTAGTGGGAATGGCAAACAGAGTTGGTTTGACAATAATCTCAACAAAGCTCATCTCGATTACTTCTACTGGTTACTTGTTGGACTTAGTGCTCTTGGGTTGGCTGCTTTCTTATTTTCTACCAGAACTTACATCTATAACAAGGGGAACACCATTTGTGGGTGGAGAGTTCCCGAATCCCTAGATTTCAGGATTCCTTAG

Coding sequence (CDS)

ATGGAGGTTCCTCTATTGGATGAGACGGTGGAGGGTGCAGTTGATTACAATGGCAACCCAGTCTGTAGATCCAATTCCGGCGGCTGGAGATCTGCCTCCCTCATTATAGGAGTGGAGGTTGCTGAGAGATTTGCCTACTATGGAGTTTCCTCCAACTTGATTAACTTCTTGACGGACCAGCTTCAGCAGTCAACGGTCACGGCAGCTAAGAATGTCAATGCCTGGTCCGGTACCGCCTCCTTGCTACCTTTACTCGGCGCCTTTCTGGCTGATTCATTCCTAGGACGATACTGGACTATTGTTCTTTCCTCTGCCCTCTACATTCTGGTTCCTCCACTACTTTATTCTCTTGTTTTGTGTGCTTCTCCTTTTCCATTCCCATTTCGCAACTATAAAGTTTTACAAGATTATGCAAGTTTCTGGGGCCTTGGGTTGTTGACCATGTCTGCATTGATATCTTCTCCAAGCACTTCTGCCTGCCAACAGACTGAAAAATCCTTACCATGTTCTCCCAATCTTTTCCAAGTTATTTTGTTTTTCTTCTCCTTATATTTGGTCGGATTGGCTCAAGGGGGACACAAGCCTTGCGTCCAAGCTTTTGGAGCCGACCAATTTGATGGACAACATCCAGAAGAGAGCATATCTAAAAGCTCTTTCTTCAATTGGTGGTATTTTGGTATCTCTTTGGCCACTTTTGCAACTGTTAATATCTTGAACTATGTGCAAGATAACCTCAGCTGGAGTCTTGGTTTTGGAATTCCTTGTATTGCTATGGTTTTAGCACTTGTAGTCTTCTTCCTTGGAACTAGGACGTACAGGTTCAGTAACAGAGGGGATGAGGAAAACCCATTTGTAAGAATTGGACGGGTGTTTATCACTGCAGTAAAAAATTGGCGAGTGAATTCTTCAGAAATGGCTCGTGAAGAGGAAATCCGTGGCCTTTTACCTCACCATAGCTCTAAACAGTTGAGGTTTCTCGATAAGGCTTTGATCGCACCTAATAGTTTGAAGGAAGACGGTCGTGCATGTAGCATCAGTGAAGTTGAGGAAGCAAAAGCAGTCCTGAGGCTTGTTCCGATATGGGTGACATGCTTAGCATTTGCTATTGTATTCTCTCAGTCATCAACTTTCTTTACAAAACAAGGAGTCACAATGGATAGATCAATAGCTTTTGGGTTTGAAGTACCGGCTGCTTCACTTCAATCTTTTATCAGTCTAGCCATTGTCATTTCCCTTATGATATATGATCGCATACTCATTCCGATTGCAAGAAATTTCACTGGGAAACCTTCTGGAATAACAACGCTACAAAGAATTGGATTTGGGATGCTACTATCTGCTATTTCCATGGTCATTGCTGCATTAGTTGAGATTAAAAGGCTTAAAACAGCTCAAGAATATGGTTTGGTTGATCTGCCTAAGGCGACTGTTCCTTTGAGTATATGGTGGCTGGTTCCTCAGTATGTTCTGTTTGGAGTTGCCGATGCTTTCACCATGGTTGGGTTGCAGGAGTTTTTTTATGACCAAGTTCCGAGTGGATTAAGAAGCATTGGTCTCTCCTTGTATTTGAGTATCTTTGGGATTGGCAACTTTTTAAGCAGCTTTCTCATCTCTGCCATTGAAAAATTAACTAGTGGGAATGGCAAACAGAGTTGGTTTGACAATAATCTCAACAAAGCTCATCTCGATTACTTCTACTGGTTACTTGTTGGACTTAGTGCTCTTGGGTTGGCTGCTTTCTTATTTTCTACCAGAACTTACATCTATAACAAGGGGAACACCATTTGTGGGTGGAGAGTTCCCGAATCCCTAGATTTCAGGATTCCTTAG

Protein sequence

MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLCASPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFFFSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNYVQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRVNSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPIWVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILIPIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAIEKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNTICGWRVPESLDFRIP
Homology
BLAST of MELO3C005483 vs. NCBI nr
Match: XP_008437003.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo] >XP_008437004.1 PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo] >KAA0043473.1 protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 562/595 (94.45%), Postives = 563/595 (94.62%), Query Frame = 0

Query: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLC 120
           LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYIL           
Sbjct: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYIL----------- 120

Query: 121 ASPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF 180
                                GLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF
Sbjct: 121 ---------------------GLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF 180

Query: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY 240
           FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY
Sbjct: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY 240

Query: 241 VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV 300
           VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV
Sbjct: 241 VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV 300

Query: 301 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 360
           NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI
Sbjct: 301 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 360

Query: 361 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 420
           WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI
Sbjct: 361 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 420

Query: 421 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480
           PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS
Sbjct: 421 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480

Query: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540
           IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI
Sbjct: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540

Query: 541 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNTI 596
           EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNT+
Sbjct: 541 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNTM 563

BLAST of MELO3C005483 vs. NCBI nr
Match: XP_031741607.1 (protein NRT1/ PTR FAMILY 5.10 isoform X2 [Cucumis sativus])

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 554/594 (93.27%), Postives = 562/594 (94.61%), Query Frame = 0

Query: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           MEVPLLDETVEGAV YNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEVPLLDETVEGAVGYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLC 120
           LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILV PLLY LVLC
Sbjct: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVLPLLYPLVLC 120

Query: 121 ASPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF 180
           A  F   F   K    Y    GLGLLTMSALI SPSTSACQQTEKSLPCSPNLFQVILFF
Sbjct: 121 AFFFHSQFPTIKF---YKRMQGLGLLTMSALIPSPSTSACQQTEKSLPCSPNLFQVILFF 180

Query: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY 240
           FSLYLVGLAQGGHKPCVQAFGADQFDGQHP+ES SKSSFFNWWYFGISLATFATVNILNY
Sbjct: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPKESRSKSSFFNWWYFGISLATFATVNILNY 240

Query: 241 VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV 300
           VQDNLSWSLGFGIPCIAMVLALVVF LGTRTYRFSNRGDEENPFVRIGRVF+TAVKNWRV
Sbjct: 241 VQDNLSWSLGFGIPCIAMVLALVVFLLGTRTYRFSNRGDEENPFVRIGRVFVTAVKNWRV 300

Query: 301 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 360
           NSSEMA EEEIRGLLPHHSSKQLRFLDKALI PNSLKEDGRACSISEVEEAKAVLRLVPI
Sbjct: 301 NSSEMAHEEEIRGLLPHHSSKQLRFLDKALITPNSLKEDGRACSISEVEEAKAVLRLVPI 360

Query: 361 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 420
           WV CLAFAIVFSQSSTFF KQGVTMDRSI FGFEVP+ASLQSFISL+IVISL+IYDRILI
Sbjct: 361 WVACLAFAIVFSQSSTFFIKQGVTMDRSITFGFEVPSASLQSFISLSIVISLLIYDRILI 420

Query: 421 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480
           PIARNFTGKPSGIT LQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVD PKATVPLS
Sbjct: 421 PIARNFTGKPSGITMLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDQPKATVPLS 480

Query: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540
           IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI
Sbjct: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540

Query: 541 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNT 595
           EKLTSG+GKQSWFDNNLNKAHLDYFYWLLVGLS +GLAAF+F  RTYIYNKGNT
Sbjct: 541 EKLTSGDGKQSWFDNNLNKAHLDYFYWLLVGLSVIGLAAFVFCARTYIYNKGNT 591

BLAST of MELO3C005483 vs. NCBI nr
Match: XP_031741606.1 (protein NRT1/ PTR FAMILY 5.10 isoform X1 [Cucumis sativus] >KAE8647915.1 hypothetical protein Csa_000486 [Cucumis sativus])

HSP 1 Score: 1049.7 bits (2713), Expect = 1.0e-302
Identity = 540/594 (90.91%), Postives = 548/594 (92.26%), Query Frame = 0

Query: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           MEVPLLDETVEGAV YNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEVPLLDETVEGAVGYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLC 120
           LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYIL           
Sbjct: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYIL----------- 120

Query: 121 ASPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF 180
                                GLGLLTMSALI SPSTSACQQTEKSLPCSPNLFQVILFF
Sbjct: 121 ---------------------GLGLLTMSALIPSPSTSACQQTEKSLPCSPNLFQVILFF 180

Query: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY 240
           FSLYLVGLAQGGHKPCVQAFGADQFDGQHP+ES SKSSFFNWWYFGISLATFATVNILNY
Sbjct: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPKESRSKSSFFNWWYFGISLATFATVNILNY 240

Query: 241 VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV 300
           VQDNLSWSLGFGIPCIAMVLALVVF LGTRTYRFSNRGDEENPFVRIGRVF+TAVKNWRV
Sbjct: 241 VQDNLSWSLGFGIPCIAMVLALVVFLLGTRTYRFSNRGDEENPFVRIGRVFVTAVKNWRV 300

Query: 301 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 360
           NSSEMA EEEIRGLLPHHSSKQLRFLDKALI PNSLKEDGRACSISEVEEAKAVLRLVPI
Sbjct: 301 NSSEMAHEEEIRGLLPHHSSKQLRFLDKALITPNSLKEDGRACSISEVEEAKAVLRLVPI 360

Query: 361 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 420
           WV CLAFAIVFSQSSTFF KQGVTMDRSI FGFEVP+ASLQSFISL+IVISL+IYDRILI
Sbjct: 361 WVACLAFAIVFSQSSTFFIKQGVTMDRSITFGFEVPSASLQSFISLSIVISLLIYDRILI 420

Query: 421 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480
           PIARNFTGKPSGIT LQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVD PKATVPLS
Sbjct: 421 PIARNFTGKPSGITMLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDQPKATVPLS 480

Query: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540
           IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI
Sbjct: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540

Query: 541 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNT 595
           EKLTSG+GKQSWFDNNLNKAHLDYFYWLLVGLS +GLAAF+F  RTYIYNKGNT
Sbjct: 541 EKLTSGDGKQSWFDNNLNKAHLDYFYWLLVGLSVIGLAAFVFCARTYIYNKGNT 562

BLAST of MELO3C005483 vs. NCBI nr
Match: XP_038877113.1 (protein NRT1/ PTR FAMILY 5.10 [Benincasa hispida] >XP_038877114.1 protein NRT1/ PTR FAMILY 5.10 [Benincasa hispida] >XP_038877115.1 protein NRT1/ PTR FAMILY 5.10 [Benincasa hispida])

HSP 1 Score: 983.0 bits (2540), Expect = 1.1e-282
Identity = 505/594 (85.02%), Postives = 531/594 (89.39%), Query Frame = 0

Query: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           ME  LLDETVEGAVDYNG+PVCRSNSGGWRSA+LIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEALLLDETVEGAVDYNGHPVCRSNSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLC 120
           LQQST TAAKNVNAWSGTASLLPLLGAFLADSFLGRY TIVLSSALYIL           
Sbjct: 61  LQQSTATAAKNVNAWSGTASLLPLLGAFLADSFLGRYRTIVLSSALYIL----------- 120

Query: 121 ASPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF 180
                                GLGLLTMSA++ SPS SACQQT+KSLPCSPNL +VILFF
Sbjct: 121 ---------------------GLGLLTMSAMLPSPSISACQQTDKSLPCSPNLVRVILFF 180

Query: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY 240
           FSLYLV L+QGGHKPCVQAFGADQFDGQHP+ES +KSSFFNWWYFGISLATF TV IL+Y
Sbjct: 181 FSLYLVALSQGGHKPCVQAFGADQFDGQHPKESKAKSSFFNWWYFGISLATFPTVCILSY 240

Query: 241 VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV 300
           VQDNLSWSLGFGIPCIAMV ALV+F LGTRTYRFS+RGDEENPFVRIGRVFI AV+NWRV
Sbjct: 241 VQDNLSWSLGFGIPCIAMVFALVIFLLGTRTYRFSSRGDEENPFVRIGRVFIIAVRNWRV 300

Query: 301 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 360
           NSSE+AREEEIRGLLPHH+SKQ RFL+KALI P+S KED   CSISEVEEAKAVLRLVPI
Sbjct: 301 NSSEIAREEEIRGLLPHHNSKQFRFLNKALIVPDSSKEDSHVCSISEVEEAKAVLRLVPI 360

Query: 361 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 420
           W+TCLA+A+VFSQSSTFFTKQGVTMDRS+  GF+VPAASLQSFIS+AIV SL IYDRILI
Sbjct: 361 WLTCLAYAVVFSQSSTFFTKQGVTMDRSLILGFKVPAASLQSFISVAIVTSLPIYDRILI 420

Query: 421 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480
           PIARNFTGKPSGIT LQRIGFGMLLS ISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS
Sbjct: 421 PIARNFTGKPSGITMLQRIGFGMLLSVISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480

Query: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540
           IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLIS I
Sbjct: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISVI 540

Query: 541 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNT 595
           EKLTSG+GKQSW D+NLNKAHLDYFYWLLVGLSA+GLAAFL S RTYIYNK NT
Sbjct: 541 EKLTSGDGKQSWLDSNLNKAHLDYFYWLLVGLSAIGLAAFLCSARTYIYNKANT 562

BLAST of MELO3C005483 vs. NCBI nr
Match: XP_022973964.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita maxima])

HSP 1 Score: 960.3 bits (2481), Expect = 8.0e-276
Identity = 493/594 (83.00%), Postives = 525/594 (88.38%), Query Frame = 0

Query: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           ME PLL++TVEGAVDYNG PV RS+SGGWRSA+LIIGVEVAERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLC 120
           L QST TAA+NVNAWSG ASLLPLLGAFLADSFLGRY TI LS+ LYIL           
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYIL----------- 120

Query: 121 ASPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF 180
                                GLGLLT+SA + SP  S CQQTEKSLPCSP LFQVILFF
Sbjct: 121 ---------------------GLGLLTVSATLHSPRISGCQQTEKSLPCSPGLFQVILFF 180

Query: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY 240
            SLY+V  AQGGHKPCVQAFGADQFDGQHPEES +KSSFFNWWYF ISLATFATVNILNY
Sbjct: 181 SSLYVVAFAQGGHKPCVQAFGADQFDGQHPEESKAKSSFFNWWYFCISLATFATVNILNY 240

Query: 241 VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV 300
           VQDNLSWSLGFGIPCI+MVLALVVF LGTRTYRF NRGDEENPFVRIGRVFITAV+NWRV
Sbjct: 241 VQDNLSWSLGFGIPCISMVLALVVFLLGTRTYRFINRGDEENPFVRIGRVFITAVRNWRV 300

Query: 301 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 360
           NSSE+A EEEIRGLLPHHSS+Q RFL+KALI PNS KE G  CSISEVEEAKAVLRLVPI
Sbjct: 301 NSSEIAHEEEIRGLLPHHSSQQFRFLNKALIVPNSSKEGGHTCSISEVEEAKAVLRLVPI 360

Query: 361 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 420
           WVTCLA+AIVFSQSSTFFTKQGVTM+RSI  GF+VPAASLQSFISL+IV+SL+IYDRILI
Sbjct: 361 WVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPGFDVPAASLQSFISLSIVVSLLIYDRILI 420

Query: 421 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480
           PIARNFTGKPSGIT LQRIGFGMLLSA+SMVIAALVEIKRL+TAQEYGLVDLPKATVPLS
Sbjct: 421 PIARNFTGKPSGITMLQRIGFGMLLSALSMVIAALVEIKRLETAQEYGLVDLPKATVPLS 480

Query: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540
           IWWLVPQYVLFGVADAFTMVGLQEFFYDQ PSGLRSIGLSLYLSIFG+GNFLSSFLISAI
Sbjct: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQAPSGLRSIGLSLYLSIFGVGNFLSSFLISAI 540

Query: 541 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNT 595
           EKL+SG+GK+SWF++NLNKAHLDYFYWLL GLSA+GLAAFL   RTYIY+KGNT
Sbjct: 541 EKLSSGDGKESWFNSNLNKAHLDYFYWLLAGLSAIGLAAFLCFARTYIYSKGNT 562

BLAST of MELO3C005483 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 5.5e-203
Identity = 369/591 (62.44%), Postives = 447/591 (75.63%), Query Frame = 0

Query: 4   PLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLQQ 63
           PLL  T    VDY   P  +S+SGGWRSA  IIGVEVAERFAYYG+SSNLI +LT  L Q
Sbjct: 13  PLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQ 72

Query: 64  STVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLCASP 123
           ST  AA NVNAWSGTASLLPLLGAF+ADSFLGR+ TI+ +SALYI+              
Sbjct: 73  STAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIV-------------- 132

Query: 124 FPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFFFSL 183
                             GLG+LT+SA+I S     C+ +     CSP  FQVI FF +L
Sbjct: 133 ------------------GLGVLTLSAMIPSD----CKVSNLLSSCSPR-FQVITFFSAL 192

Query: 184 YLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNYVQD 243
           YLV LAQGGHKPCVQAFGADQFD + PEE  +KSSFFNWWYFG+   T  T+ +LNY+QD
Sbjct: 193 YLVALAQGGHKPCVQAFGADQFDEKEPEECKAKSSFFNWWYFGMCFGTLTTLWVLNYIQD 252

Query: 244 NLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRVNSS 303
           NLSW+LGFGIPCIAMV+ALVV  LGT TYRFS R ++++PFVRIG V++ AVKNW V++ 
Sbjct: 253 NLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRREDQSPFVRIGNVYVAAVKNWSVSAL 312

Query: 304 EMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPIWVT 363
           ++A  EE  GL+   SS+Q  FL+KAL+A N       +CSI E+EEAK+VLRL PIW+T
Sbjct: 313 DVAAAEERLGLVSCSSSQQFSFLNKALVAKNG------SCSIDELEEAKSVLRLAPIWLT 372

Query: 364 CLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILIPIA 423
           CL +A+VF+QS TFFTKQG TM+RSI  G+++  A+LQSFISL+IVI + IYDR+LIPIA
Sbjct: 373 CLVYAVVFAQSPTFFTKQGATMERSITPGYKISPATLQSFISLSIVIFIPIYDRVLIPIA 432

Query: 424 RNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLSIWW 483
           R+FT KP GIT LQRIG G+ LS ++MV+AALVE+KRLKTA +YGLVD P ATVP+S+WW
Sbjct: 433 RSFTHKPGGITMLQRIGTGIFLSFLAMVVAALVEMKRLKTAADYGLVDSPDATVPMSVWW 492

Query: 484 LVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAIEKL 543
           LVPQYVLFG+ D F MVGLQEFFYDQVP+ LRS+GL+LYLSIFGIGNFLSSF+IS IEK 
Sbjct: 493 LVPQYVLFGITDVFAMVGLQEFFYDQVPNELRSVGLALYLSIFGIGNFLSSFMISIIEKA 552

Query: 544 TSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNT 595
           TS +G+ SWF NNLN+AHLDYFYWLL  LS +GLA++L+  ++Y+  + +T
Sbjct: 553 TSQSGQASWFANNLNQAHLDYFYWLLACLSFIGLASYLYVAKSYVSKRLDT 556

BLAST of MELO3C005483 vs. ExPASy Swiss-Prot
Match: Q0WSZ6 (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 604.0 bits (1556), Expect = 1.9e-171
Identity = 310/593 (52.28%), Postives = 413/593 (69.65%), Query Frame = 0

Query: 5   LLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLQQS 64
           L +E V  AVD+ G    RS +G WR+A  IIGVEVAERFA YG+ SNLI++LT  L QS
Sbjct: 9   LQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQS 68

Query: 65  TVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLCASPF 124
           T  AA NVNAWSG +++LPLLGAF+AD+FLGRY TI+++S +Y+L               
Sbjct: 69  TAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVL--------------- 128

Query: 125 PFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFFFSLY 184
                            GL  LT+SA +   +T         +  SP+ F   LFFFSLY
Sbjct: 129 -----------------GLAFLTLSAFLIPNNT--------EVTSSPSSFLNALFFFSLY 188

Query: 185 LVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNYVQDN 244
           LV + Q GHKPCVQAFGADQFD ++P+E+  +SSFFNWWY  +       + ++ Y+Q+N
Sbjct: 189 LVAIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFNWWYLSMCAGIGLAILVVVYIQEN 248

Query: 245 LSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEE--NPFVRIGRVFITAVKNWRVNS 304
           +SW+LGFGIPC+ MV++LV+F LG ++YRFS    EE  NPF RIGRVF  A KN R+NS
Sbjct: 249 VSWALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEEETNPFTRIGRVFFVAFKNQRLNS 308

Query: 305 SEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPIWV 364
           S++ + E I       S ++L FL+KAL+ PN   E   AC   +VE+A A++RL+P+W+
Sbjct: 309 SDLCKVELIEANRSQESPEELSFLNKALLVPNDSDEGEVACKSRDVEDATALVRLIPVWL 368

Query: 365 TCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILIPI 424
           T LA+AI F+Q  TFFTKQGVTM+R+I  G E+P ASLQ  IS++IV+ + IYDR+L+PI
Sbjct: 369 TTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPASLQVLISISIVLFVPIYDRVLVPI 428

Query: 425 ARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLSIW 484
            R+ T  P GITTL+RIG GM+L+ ++MV+AALVE KRL+TA+EYGL+D PK T+P+SIW
Sbjct: 429 GRSITKDPCGITTLKRIGTGMVLATLTMVVAALVESKRLETAKEYGLIDQPKTTLPMSIW 488

Query: 485 WLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAIEK 544
           WL PQY+L G+AD  T+VG+QEFFY QVP+ LRS+GL++YLS  G+G+ LSS LI  I+ 
Sbjct: 489 WLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGLAIYLSAMGVGSLLSSLLIYLIDL 548

Query: 545 LTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNTI 596
            T G+   SWF++NLN+AHLDYFYWLL  +SA+G   FLF +++YIY + + +
Sbjct: 549 ATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAVGFFTFLFISKSYIYRRVDVV 561

BLAST of MELO3C005483 vs. ExPASy Swiss-Prot
Match: Q9C7U1 (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 1.9e-171
Identity = 322/590 (54.58%), Postives = 415/590 (70.34%), Query Frame = 0

Query: 7   DETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLQQSTV 66
           +E VE +VD+ GNP  RS+SG W+S+   +  EVAE+FAY+G++SNLI + T+ L +ST 
Sbjct: 16  NENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTA 75

Query: 67  TAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLCASPFPF 126
            AA NVN W GTA+ LPL+   +ADSFLGR+ TI+L+S+ YI+                 
Sbjct: 76  VAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIM----------------- 135

Query: 127 PFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFFFSLYLV 186
                          GLGLLT SA I  PS    Q+T +S  C   + +VI+FF +LYL+
Sbjct: 136 ---------------GLGLLTFSATI--PSLCNDQETRES--CVSQV-KVIIFFCALYLI 195

Query: 187 GLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNYVQDNLS 246
            L +GG K C++AFGADQFD Q P ES +KSS+FNW YF IS+    T  + NYVQ+NLS
Sbjct: 196 ALGEGGFKVCLRAFGADQFDEQDPNESKAKSSYFNWLYFAISIGILTTRLVTNYVQENLS 255

Query: 247 WSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGD------EENPFVRIGRVFITAVKNWRV 306
           W+LG+ IPC++M+LAL +F LG +TYRFS  G+       +NPFVRIGRVF+ A +N R 
Sbjct: 256 WALGYAIPCLSMMLALFLFLLGIKTYRFSTGGEGRQGKKHDNPFVRIGRVFVAAARNRRQ 315

Query: 307 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 366
             S+         LLP+ S+K+ RFLD+A+I          +C   EVEEAKAVL L+PI
Sbjct: 316 TPSDTCL------LLPNESTKKFRFLDRAVI----------SCDSYEVEEAKAVLSLIPI 375

Query: 367 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 426
           W+  L F IVF+QS TFFTKQG TMDRSI+   +VPAA+LQ FISLAI++ + IYDR+ +
Sbjct: 376 WLCSLVFGIVFAQSPTFFTKQGSTMDRSISSTLQVPAATLQCFISLAILVFIPIYDRLFV 435

Query: 427 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 486
           PIAR+ T KP+GITTLQRI  G+ LS ISMVIAALVE+KRLKTA+++GLVD PKATVP+S
Sbjct: 436 PIARSITRKPAGITTLQRISTGIFLSIISMVIAALVEMKRLKTARDHGLVDSPKATVPMS 495

Query: 487 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 546
           + WL+PQY+LFGV+D FTMVGLQEFFY +VP  LRS+GL+LYLSI GIGNFLSSF++S I
Sbjct: 496 VCWLIPQYILFGVSDVFTMVGLQEFFYGEVPPQLRSMGLALYLSIIGIGNFLSSFMVSVI 552

Query: 547 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYN 591
           E+ TS +G+ SWF NNLN+AHLDYFYWLL  LS+L     ++  ++Y+YN
Sbjct: 556 EEATSQSGQVSWFSNNLNQAHLDYFYWLLACLSSLAFIFTVYFAKSYLYN 552

BLAST of MELO3C005483 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 602.1 bits (1551), Expect = 7.2e-171
Identity = 310/592 (52.36%), Postives = 410/592 (69.26%), Query Frame = 0

Query: 2   EVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 61
           E+ L +E V  AVD+ G    RSN+G WR+A  IIGVEVAERFAYYG+ SNLI++LT  L
Sbjct: 6   EISLQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPL 65

Query: 62  QQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLCA 121
            +ST  AA NVNAWSG A+LLP+LGAF+AD+FLGRY TI++SS +Y+L            
Sbjct: 66  GESTAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVL------------ 125

Query: 122 SPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFFF 181
                               GL  LT+SA +   +T     T          F  +LFFF
Sbjct: 126 --------------------GLAFLTLSAFLIPNTTEVTSSTSS--------FLNVLFFF 185

Query: 182 SLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNYV 241
           SLYLV + Q GHKPCVQAFGADQFD +  +E   +SSFFNWWY  +S      + ++ Y+
Sbjct: 186 SLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWYLSLSAGICFAILVVVYI 245

Query: 242 QDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEE--NPFVRIGRVFITAVKNWR 301
           Q+  SW+ GFGIPC+ MV++LV+F  G R YR+S R  EE  NPF RIGRVF  A+KN R
Sbjct: 246 QEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEINPFTRIGRVFFVALKNQR 305

Query: 302 VNSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVP 361
           ++SS++ + E    L  + S ++  F +KAL+ PN   +   A   S+VE+A A++RL+P
Sbjct: 306 LSSSDLCKVE----LEANTSPEKQSFFNKALLVPNDSSQGENASKSSDVEDATALIRLIP 365

Query: 362 IWVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRIL 421
           +W T LA+AI ++Q  TFFTKQGVTMDR+I  G ++P ASLQ FI ++IV+ + IYDR+ 
Sbjct: 366 VWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPPASLQVFIGISIVLFVPIYDRVF 425

Query: 422 IPIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPL 481
           +PIAR  T +P GITTL+RIG G++LS I+MVIAALVE KRL+TA+E+GL+D P+AT+P+
Sbjct: 426 VPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKRLETAKEHGLIDQPEATLPM 485

Query: 482 SIWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISA 541
           SIWWL+PQY+L G+AD +T+VG+QEFFY QVP+ LRSIGL+LYLS  G+G+ LSS LIS 
Sbjct: 486 SIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLALYLSALGVGSLLSSLLISL 545

Query: 542 IEKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNK 592
           I+  T G+   SWF++NLN+AHLDYFYWLL  +SA+G   FLF +++YIY +
Sbjct: 546 IDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGFFTFLFISKSYIYRR 553

BLAST of MELO3C005483 vs. ExPASy Swiss-Prot
Match: Q9SK96 (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 7.0e-166
Identity = 307/598 (51.34%), Postives = 408/598 (68.23%), Query Frame = 0

Query: 2   EVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 61
           E  L++++V  +VD+ G P  +S++GGWRSA  IIGVEV ERFAY+G+ SNLI +LT  L
Sbjct: 7   EAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPL 66

Query: 62  QQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLCA 121
            QST TAA NVN WSGTAS+LP+LGAF+AD++LGRY TIV++S +YIL            
Sbjct: 67  GQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYIL------------ 126

Query: 122 SPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFFF 181
                               GLGLLT+S+++     S  +Q  ++    P  +  ILFF 
Sbjct: 127 --------------------GLGLLTLSSILILMGLSEQRQHNRNASAKPFFWVNILFFC 186

Query: 182 SLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNYV 241
           SLYLV + QGGHKPCVQAFGADQFD   P+E IS+ SFFNWW+  +S     ++ ++ YV
Sbjct: 187 SLYLVAIGQGGHKPCVQAFGADQFDVGDPKERISRGSFFNWWFLSLSAGITLSIIVVVYV 246

Query: 242 QDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEE---NPFVRIGRVFITAVKNW 301
           QDN++W+LGFGIPC+ MV+AL +F  G +TYR+  RGD E   N F RIGRVF+ A KN 
Sbjct: 247 QDNVNWALGFGIPCLFMVMALALFLFGRKTYRYP-RGDREGKNNAFARIGRVFLVAFKNR 306

Query: 302 RVNSS-----EMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKA 361
           ++  +     E+   ++ +G        QL FL KAL+      E    CS  +VE+A A
Sbjct: 307 KLKLTHSGQLEVGSYKKCKG--------QLEFLAKALLPGEGGVE---PCSSRDVEDAMA 366

Query: 362 VLRLVPIWVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLM 421
           ++RL+PIW+T +   I ++Q +TFFTKQGVT+DR I  GFE+P AS Q+ I L+I IS+ 
Sbjct: 367 LVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKILPGFEIPPASFQALIGLSIFISVP 426

Query: 422 IYDRILIPIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLP 481
            Y+R+ +P+AR  T KPSGIT LQRIG GM+LS+++MV+AALVE+KRL+TA+E+GLVD P
Sbjct: 427 TYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLNMVVAALVEMKRLETAKEHGLVDRP 486

Query: 482 KATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLS 541
            AT+P+SIWW VPQY+L G+ D F++VG QEFFYDQVP+ LRSIGL+L LS  G+ +FLS
Sbjct: 487 DATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQVPTELRSIGLALSLSAMGLASFLS 546

Query: 542 SFLISAIEKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNK 592
            FLI+ I   T  NG  SWF+ NLN+AH+DYFYWLL   +A+G  AFL  +R Y+Y +
Sbjct: 547 GFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLLAAFTAIGFLAFLLLSRLYVYRR 560

BLAST of MELO3C005483 vs. ExPASy TrEMBL
Match: A0A1S3ATI3 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103482564 PE=3 SV=1)

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 562/595 (94.45%), Postives = 563/595 (94.62%), Query Frame = 0

Query: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLC 120
           LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYIL           
Sbjct: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYIL----------- 120

Query: 121 ASPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF 180
                                GLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF
Sbjct: 121 ---------------------GLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF 180

Query: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY 240
           FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY
Sbjct: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY 240

Query: 241 VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV 300
           VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV
Sbjct: 241 VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV 300

Query: 301 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 360
           NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI
Sbjct: 301 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 360

Query: 361 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 420
           WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI
Sbjct: 361 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 420

Query: 421 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480
           PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS
Sbjct: 421 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480

Query: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540
           IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI
Sbjct: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540

Query: 541 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNTI 596
           EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNT+
Sbjct: 541 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNTM 563

BLAST of MELO3C005483 vs. ExPASy TrEMBL
Match: A0A5A7TJ76 (Protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00110 PE=3 SV=1)

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 562/595 (94.45%), Postives = 563/595 (94.62%), Query Frame = 0

Query: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLC 120
           LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYIL           
Sbjct: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYIL----------- 120

Query: 121 ASPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF 180
                                GLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF
Sbjct: 121 ---------------------GLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF 180

Query: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY 240
           FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY
Sbjct: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY 240

Query: 241 VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV 300
           VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV
Sbjct: 241 VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV 300

Query: 301 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 360
           NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI
Sbjct: 301 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 360

Query: 361 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 420
           WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI
Sbjct: 361 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 420

Query: 421 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480
           PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS
Sbjct: 421 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480

Query: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540
           IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI
Sbjct: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540

Query: 541 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNTI 596
           EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNT+
Sbjct: 541 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNTM 563

BLAST of MELO3C005483 vs. ExPASy TrEMBL
Match: A0A6J1IG54 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111472583 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.9e-276
Identity = 493/594 (83.00%), Postives = 525/594 (88.38%), Query Frame = 0

Query: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           ME PLL++TVEGAVDYNG PV RS+SGGWRSA+LIIGVEVAERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLC 120
           L QST TAA+NVNAWSG ASLLPLLGAFLADSFLGRY TI LS+ LYIL           
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYIL----------- 120

Query: 121 ASPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF 180
                                GLGLLT+SA + SP  S CQQTEKSLPCSP LFQVILFF
Sbjct: 121 ---------------------GLGLLTVSATLHSPRISGCQQTEKSLPCSPGLFQVILFF 180

Query: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY 240
            SLY+V  AQGGHKPCVQAFGADQFDGQHPEES +KSSFFNWWYF ISLATFATVNILNY
Sbjct: 181 SSLYVVAFAQGGHKPCVQAFGADQFDGQHPEESKAKSSFFNWWYFCISLATFATVNILNY 240

Query: 241 VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV 300
           VQDNLSWSLGFGIPCI+MVLALVVF LGTRTYRF NRGDEENPFVRIGRVFITAV+NWRV
Sbjct: 241 VQDNLSWSLGFGIPCISMVLALVVFLLGTRTYRFINRGDEENPFVRIGRVFITAVRNWRV 300

Query: 301 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 360
           NSSE+A EEEIRGLLPHHSS+Q RFL+KALI PNS KE G  CSISEVEEAKAVLRLVPI
Sbjct: 301 NSSEIAHEEEIRGLLPHHSSQQFRFLNKALIVPNSSKEGGHTCSISEVEEAKAVLRLVPI 360

Query: 361 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 420
           WVTCLA+AIVFSQSSTFFTKQGVTM+RSI  GF+VPAASLQSFISL+IV+SL+IYDRILI
Sbjct: 361 WVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPGFDVPAASLQSFISLSIVVSLLIYDRILI 420

Query: 421 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480
           PIARNFTGKPSGIT LQRIGFGMLLSA+SMVIAALVEIKRL+TAQEYGLVDLPKATVPLS
Sbjct: 421 PIARNFTGKPSGITMLQRIGFGMLLSALSMVIAALVEIKRLETAQEYGLVDLPKATVPLS 480

Query: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540
           IWWLVPQYVLFGVADAFTMVGLQEFFYDQ PSGLRSIGLSLYLSIFG+GNFLSSFLISAI
Sbjct: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQAPSGLRSIGLSLYLSIFGVGNFLSSFLISAI 540

Query: 541 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNT 595
           EKL+SG+GK+SWF++NLNKAHLDYFYWLL GLSA+GLAAFL   RTYIY+KGNT
Sbjct: 541 EKLSSGDGKESWFNSNLNKAHLDYFYWLLAGLSAIGLAAFLCFARTYIYSKGNT 562

BLAST of MELO3C005483 vs. ExPASy TrEMBL
Match: A0A6J1EHW2 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432710 PE=3 SV=1)

HSP 1 Score: 953.0 bits (2462), Expect = 6.1e-274
Identity = 490/594 (82.49%), Postives = 522/594 (87.88%), Query Frame = 0

Query: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           ME PLL++TVE AVDYNG PV RS+SGGWRSA+LIIGVEVAERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVECAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLC 120
           L QST TAA+NVNAWSG ASLLPLLGAFLADSFLGRY TI LS+ LYIL           
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYIL----------- 120

Query: 121 ASPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF 180
                                GLGLLT+SA + SPS S CQQTEKSLPCSP LFQVILFF
Sbjct: 121 ---------------------GLGLLTVSATLHSPSISGCQQTEKSLPCSPGLFQVILFF 180

Query: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY 240
           FSLY+V  AQGGHKPCVQAFGADQFD QHPEES +KSSFFNWWYF ISLATFATVNILNY
Sbjct: 181 FSLYVVAFAQGGHKPCVQAFGADQFDAQHPEESKAKSSFFNWWYFCISLATFATVNILNY 240

Query: 241 VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV 300
           VQDNLSWSLGFGIPCI+MVLALVVF LGTRTYRF NRGDEENPFVRIGRVFITAV+NWRV
Sbjct: 241 VQDNLSWSLGFGIPCISMVLALVVFLLGTRTYRFINRGDEENPFVRIGRVFITAVRNWRV 300

Query: 301 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 360
           NSSE+A EEEIRGLLPHHSS+Q RFL+KAL+ PNS KE G  C+ISEVEEAKAVLRLVPI
Sbjct: 301 NSSEIAHEEEIRGLLPHHSSQQFRFLNKALLVPNSSKEGGHTCTISEVEEAKAVLRLVPI 360

Query: 361 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 420
           WVTCLA+AIVFSQSSTFFTKQGVTM+RSI  GF+VPAASLQSFISL+IV+SL IYDRILI
Sbjct: 361 WVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPGFDVPAASLQSFISLSIVVSLPIYDRILI 420

Query: 421 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480
           PIARNFTGKPSGIT LQRIGFGMLLSA+SMVIAALVEIKRL+TAQEYGLVDLPKATVPLS
Sbjct: 421 PIARNFTGKPSGITMLQRIGFGMLLSALSMVIAALVEIKRLETAQEYGLVDLPKATVPLS 480

Query: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540
           IWWLVPQYVLFGVADAFTMVGLQEFFYDQ PSGLRSIGLSLYLSIFG+GNFLSSFLISAI
Sbjct: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQAPSGLRSIGLSLYLSIFGVGNFLSSFLISAI 540

Query: 541 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNT 595
           E L+SG+GK+SWF+NNLNKAHLDYFYWLL GLSA+GLAAFL   RTYIY+K NT
Sbjct: 541 ENLSSGDGKESWFNNNLNKAHLDYFYWLLAGLSAIGLAAFLCFARTYIYSKKNT 562

BLAST of MELO3C005483 vs. ExPASy TrEMBL
Match: A0A6J1EC56 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432731 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 2.0e-272
Identity = 491/609 (80.62%), Postives = 522/609 (85.71%), Query Frame = 0

Query: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60
           ME PLL++TVEGAVDY+G PV RS+SGGWRSA+LIIGVEVAERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYHGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLC 120
           L QST TAA+NVN WS  ASLLPLLGAFLADSFLGRY TI LSS LYIL           
Sbjct: 61  LGQSTATAAENVNVWSAAASLLPLLGAFLADSFLGRYRTIALSSVLYIL----------- 120

Query: 121 ASPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFF 180
                                GLGLLT+SA + SPS S CQQTEKSLPCSP LFQVILFF
Sbjct: 121 ---------------------GLGLLTVSATLHSPSISGCQQTEKSLPCSPGLFQVILFF 180

Query: 181 FSLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNY 240
            S+Y+V  AQGGHKPCVQAFGADQFD QHPEES +KSSFFNWWYF ISLATFATVNILNY
Sbjct: 181 SSVYIVAFAQGGHKPCVQAFGADQFDAQHPEESKAKSSFFNWWYFCISLATFATVNILNY 240

Query: 241 VQDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRV 300
           VQDNLSWSLGFGIPCI+MVLALVVF LGTRTYRF NRGDEENPFVRIGRVFITAV+NWRV
Sbjct: 241 VQDNLSWSLGFGIPCISMVLALVVFLLGTRTYRFINRGDEENPFVRIGRVFITAVRNWRV 300

Query: 301 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 360
           NSSE+A EEEI GLLPHHSS+Q RFL+KALI P S KE G  C+ISEVEEAKAVLRLVPI
Sbjct: 301 NSSEIAHEEEICGLLPHHSSQQFRFLNKALIVPYSSKEGGHTCTISEVEEAKAVLRLVPI 360

Query: 361 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 420
           WVTCLA+AIVFSQSSTFFTKQGVTM+RSI  GF+VPAASLQSFI L+IV+SL IYDRILI
Sbjct: 361 WVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPGFDVPAASLQSFIGLSIVVSLPIYDRILI 420

Query: 421 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 480
           PIARNFTGKPSGIT LQRIGFGMLLSA+SMVIAALVEIKRL+TAQEYGLVDLPKATVPLS
Sbjct: 421 PIARNFTGKPSGITMLQRIGFGMLLSALSMVIAALVEIKRLETAQEYGLVDLPKATVPLS 480

Query: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 540
           IWWLVPQYVLFGVADAFTMVGLQEFFYDQ PSGLRSIGLSLYLSIFG+GNFLSSFLISAI
Sbjct: 481 IWWLVPQYVLFGVADAFTMVGLQEFFYDQAPSGLRSIGLSLYLSIFGVGNFLSSFLISAI 540

Query: 541 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNT------ 600
           E L+SG+GK+SWF+NNLNKAHLDYFYWLL GLSA+GLAAFL   RTYIY KGNT      
Sbjct: 541 ENLSSGDGKESWFNNNLNKAHLDYFYWLLAGLSAVGLAAFLCFARTYIYGKGNTTLFITA 577

Query: 601 ICGWRVPES 604
           +  WRV  S
Sbjct: 601 VRNWRVNSS 577

BLAST of MELO3C005483 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 708.8 bits (1828), Expect = 3.9e-204
Identity = 369/591 (62.44%), Postives = 447/591 (75.63%), Query Frame = 0

Query: 4   PLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLQQ 63
           PLL  T    VDY   P  +S+SGGWRSA  IIGVEVAERFAYYG+SSNLI +LT  L Q
Sbjct: 13  PLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQ 72

Query: 64  STVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLCASP 123
           ST  AA NVNAWSGTASLLPLLGAF+ADSFLGR+ TI+ +SALYI+              
Sbjct: 73  STAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIV-------------- 132

Query: 124 FPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFFFSL 183
                             GLG+LT+SA+I S     C+ +     CSP  FQVI FF +L
Sbjct: 133 ------------------GLGVLTLSAMIPSD----CKVSNLLSSCSPR-FQVITFFSAL 192

Query: 184 YLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNYVQD 243
           YLV LAQGGHKPCVQAFGADQFD + PEE  +KSSFFNWWYFG+   T  T+ +LNY+QD
Sbjct: 193 YLVALAQGGHKPCVQAFGADQFDEKEPEECKAKSSFFNWWYFGMCFGTLTTLWVLNYIQD 252

Query: 244 NLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEENPFVRIGRVFITAVKNWRVNSS 303
           NLSW+LGFGIPCIAMV+ALVV  LGT TYRFS R ++++PFVRIG V++ AVKNW V++ 
Sbjct: 253 NLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRREDQSPFVRIGNVYVAAVKNWSVSAL 312

Query: 304 EMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPIWVT 363
           ++A  EE  GL+   SS+Q  FL+KAL+A N       +CSI E+EEAK+VLRL PIW+T
Sbjct: 313 DVAAAEERLGLVSCSSSQQFSFLNKALVAKNG------SCSIDELEEAKSVLRLAPIWLT 372

Query: 364 CLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILIPIA 423
           CL +A+VF+QS TFFTKQG TM+RSI  G+++  A+LQSFISL+IVI + IYDR+LIPIA
Sbjct: 373 CLVYAVVFAQSPTFFTKQGATMERSITPGYKISPATLQSFISLSIVIFIPIYDRVLIPIA 432

Query: 424 RNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLSIWW 483
           R+FT KP GIT LQRIG G+ LS ++MV+AALVE+KRLKTA +YGLVD P ATVP+S+WW
Sbjct: 433 RSFTHKPGGITMLQRIGTGIFLSFLAMVVAALVEMKRLKTAADYGLVDSPDATVPMSVWW 492

Query: 484 LVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAIEKL 543
           LVPQYVLFG+ D F MVGLQEFFYDQVP+ LRS+GL+LYLSIFGIGNFLSSF+IS IEK 
Sbjct: 493 LVPQYVLFGITDVFAMVGLQEFFYDQVPNELRSVGLALYLSIFGIGNFLSSFMISIIEKA 552

Query: 544 TSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNT 595
           TS +G+ SWF NNLN+AHLDYFYWLL  LS +GLA++L+  ++Y+  + +T
Sbjct: 553 TSQSGQASWFANNLNQAHLDYFYWLLACLSFIGLASYLYVAKSYVSKRLDT 556

BLAST of MELO3C005483 vs. TAIR 10
Match: AT1G72140.1 (Major facilitator superfamily protein )

HSP 1 Score: 604.0 bits (1556), Expect = 1.3e-172
Identity = 322/590 (54.58%), Postives = 415/590 (70.34%), Query Frame = 0

Query: 7   DETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLQQSTV 66
           +E VE +VD+ GNP  RS+SG W+S+   +  EVAE+FAY+G++SNLI + T+ L +ST 
Sbjct: 16  NENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTA 75

Query: 67  TAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLCASPFPF 126
            AA NVN W GTA+ LPL+   +ADSFLGR+ TI+L+S+ YI+                 
Sbjct: 76  VAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIM----------------- 135

Query: 127 PFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFFFSLYLV 186
                          GLGLLT SA I  PS    Q+T +S  C   + +VI+FF +LYL+
Sbjct: 136 ---------------GLGLLTFSATI--PSLCNDQETRES--CVSQV-KVIIFFCALYLI 195

Query: 187 GLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNYVQDNLS 246
            L +GG K C++AFGADQFD Q P ES +KSS+FNW YF IS+    T  + NYVQ+NLS
Sbjct: 196 ALGEGGFKVCLRAFGADQFDEQDPNESKAKSSYFNWLYFAISIGILTTRLVTNYVQENLS 255

Query: 247 WSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGD------EENPFVRIGRVFITAVKNWRV 306
           W+LG+ IPC++M+LAL +F LG +TYRFS  G+       +NPFVRIGRVF+ A +N R 
Sbjct: 256 WALGYAIPCLSMMLALFLFLLGIKTYRFSTGGEGRQGKKHDNPFVRIGRVFVAAARNRRQ 315

Query: 307 NSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPI 366
             S+         LLP+ S+K+ RFLD+A+I          +C   EVEEAKAVL L+PI
Sbjct: 316 TPSDTCL------LLPNESTKKFRFLDRAVI----------SCDSYEVEEAKAVLSLIPI 375

Query: 367 WVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILI 426
           W+  L F IVF+QS TFFTKQG TMDRSI+   +VPAA+LQ FISLAI++ + IYDR+ +
Sbjct: 376 WLCSLVFGIVFAQSPTFFTKQGSTMDRSISSTLQVPAATLQCFISLAILVFIPIYDRLFV 435

Query: 427 PIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLS 486
           PIAR+ T KP+GITTLQRI  G+ LS ISMVIAALVE+KRLKTA+++GLVD PKATVP+S
Sbjct: 436 PIARSITRKPAGITTLQRISTGIFLSIISMVIAALVEMKRLKTARDHGLVDSPKATVPMS 495

Query: 487 IWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAI 546
           + WL+PQY+LFGV+D FTMVGLQEFFY +VP  LRS+GL+LYLSI GIGNFLSSF++S I
Sbjct: 496 VCWLIPQYILFGVSDVFTMVGLQEFFYGEVPPQLRSMGLALYLSIIGIGNFLSSFMVSVI 552

Query: 547 EKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYN 591
           E+ TS +G+ SWF NNLN+AHLDYFYWLL  LS+L     ++  ++Y+YN
Sbjct: 556 EEATSQSGQVSWFSNNLNQAHLDYFYWLLACLSSLAFIFTVYFAKSYLYN 552

BLAST of MELO3C005483 vs. TAIR 10
Match: AT1G72125.1 (Major facilitator superfamily protein )

HSP 1 Score: 604.0 bits (1556), Expect = 1.3e-172
Identity = 310/593 (52.28%), Postives = 413/593 (69.65%), Query Frame = 0

Query: 5   LLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQLQQS 64
           L +E V  AVD+ G    RS +G WR+A  IIGVEVAERFA YG+ SNLI++LT  L QS
Sbjct: 9   LQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQS 68

Query: 65  TVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLCASPF 124
           T  AA NVNAWSG +++LPLLGAF+AD+FLGRY TI+++S +Y+L               
Sbjct: 69  TAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVL--------------- 128

Query: 125 PFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFFFSLY 184
                            GL  LT+SA +   +T         +  SP+ F   LFFFSLY
Sbjct: 129 -----------------GLAFLTLSAFLIPNNT--------EVTSSPSSFLNALFFFSLY 188

Query: 185 LVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNYVQDN 244
           LV + Q GHKPCVQAFGADQFD ++P+E+  +SSFFNWWY  +       + ++ Y+Q+N
Sbjct: 189 LVAIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFNWWYLSMCAGIGLAILVVVYIQEN 248

Query: 245 LSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEE--NPFVRIGRVFITAVKNWRVNS 304
           +SW+LGFGIPC+ MV++LV+F LG ++YRFS    EE  NPF RIGRVF  A KN R+NS
Sbjct: 249 VSWALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEEETNPFTRIGRVFFVAFKNQRLNS 308

Query: 305 SEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVPIWV 364
           S++ + E I       S ++L FL+KAL+ PN   E   AC   +VE+A A++RL+P+W+
Sbjct: 309 SDLCKVELIEANRSQESPEELSFLNKALLVPNDSDEGEVACKSRDVEDATALVRLIPVWL 368

Query: 365 TCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRILIPI 424
           T LA+AI F+Q  TFFTKQGVTM+R+I  G E+P ASLQ  IS++IV+ + IYDR+L+PI
Sbjct: 369 TTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPASLQVLISISIVLFVPIYDRVLVPI 428

Query: 425 ARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPLSIW 484
            R+ T  P GITTL+RIG GM+L+ ++MV+AALVE KRL+TA+EYGL+D PK T+P+SIW
Sbjct: 429 GRSITKDPCGITTLKRIGTGMVLATLTMVVAALVESKRLETAKEYGLIDQPKTTLPMSIW 488

Query: 485 WLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISAIEK 544
           WL PQY+L G+AD  T+VG+QEFFY QVP+ LRS+GL++YLS  G+G+ LSS LI  I+ 
Sbjct: 489 WLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGLAIYLSAMGVGSLLSSLLIYLIDL 548

Query: 545 LTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNKGNTI 596
            T G+   SWF++NLN+AHLDYFYWLL  +SA+G   FLF +++YIY + + +
Sbjct: 549 ATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSAVGFFTFLFISKSYIYRRVDVV 561

BLAST of MELO3C005483 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 602.1 bits (1551), Expect = 5.1e-172
Identity = 310/592 (52.36%), Postives = 410/592 (69.26%), Query Frame = 0

Query: 2   EVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 61
           E+ L +E V  AVD+ G    RSN+G WR+A  IIGVEVAERFAYYG+ SNLI++LT  L
Sbjct: 6   EISLQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPL 65

Query: 62  QQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLCA 121
            +ST  AA NVNAWSG A+LLP+LGAF+AD+FLGRY TI++SS +Y+L            
Sbjct: 66  GESTAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVL------------ 125

Query: 122 SPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFFF 181
                               GL  LT+SA +   +T     T          F  +LFFF
Sbjct: 126 --------------------GLAFLTLSAFLIPNTTEVTSSTSS--------FLNVLFFF 185

Query: 182 SLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNYV 241
           SLYLV + Q GHKPCVQAFGADQFD +  +E   +SSFFNWWY  +S      + ++ Y+
Sbjct: 186 SLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWYLSLSAGICFAILVVVYI 245

Query: 242 QDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEE--NPFVRIGRVFITAVKNWR 301
           Q+  SW+ GFGIPC+ MV++LV+F  G R YR+S R  EE  NPF RIGRVF  A+KN R
Sbjct: 246 QEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEINPFTRIGRVFFVALKNQR 305

Query: 302 VNSSEMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKAVLRLVP 361
           ++SS++ + E    L  + S ++  F +KAL+ PN   +   A   S+VE+A A++RL+P
Sbjct: 306 LSSSDLCKVE----LEANTSPEKQSFFNKALLVPNDSSQGENASKSSDVEDATALIRLIP 365

Query: 362 IWVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLMIYDRIL 421
           +W T LA+AI ++Q  TFFTKQGVTMDR+I  G ++P ASLQ FI ++IV+ + IYDR+ 
Sbjct: 366 VWFTTLAYAIPYAQYMTFFTKQGVTMDRTILPGVKIPPASLQVFIGISIVLFVPIYDRVF 425

Query: 422 IPIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLPKATVPL 481
           +PIAR  T +P GITTL+RIG G++LS I+MVIAALVE KRL+TA+E+GL+D P+AT+P+
Sbjct: 426 VPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKRLETAKEHGLIDQPEATLPM 485

Query: 482 SIWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLSSFLISA 541
           SIWWL+PQY+L G+AD +T+VG+QEFFY QVP+ LRSIGL+LYLS  G+G+ LSS LIS 
Sbjct: 486 SIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLALYLSALGVGSLLSSLLISL 545

Query: 542 IEKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNK 592
           I+  T G+   SWF++NLN+AHLDYFYWLL  +SA+G   FLF +++YIY +
Sbjct: 546 IDLATGGDAGNSWFNSNLNRAHLDYFYWLLAIVSAVGFFTFLFISKSYIYRR 553

BLAST of MELO3C005483 vs. TAIR 10
Match: AT1G22550.1 (Major facilitator superfamily protein )

HSP 1 Score: 585.5 bits (1508), Expect = 5.0e-167
Identity = 307/598 (51.34%), Postives = 408/598 (68.23%), Query Frame = 0

Query: 2   EVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQL 61
           E  L++++V  +VD+ G P  +S++GGWRSA  IIGVEV ERFAY+G+ SNLI +LT  L
Sbjct: 7   EAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPL 66

Query: 62  QQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILVPPLLYSLVLCA 121
            QST TAA NVN WSGTAS+LP+LGAF+AD++LGRY TIV++S +YIL            
Sbjct: 67  GQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYIL------------ 126

Query: 122 SPFPFPFRNYKVLQDYASFWGLGLLTMSALISSPSTSACQQTEKSLPCSPNLFQVILFFF 181
                               GLGLLT+S+++     S  +Q  ++    P  +  ILFF 
Sbjct: 127 --------------------GLGLLTLSSILILMGLSEQRQHNRNASAKPFFWVNILFFC 186

Query: 182 SLYLVGLAQGGHKPCVQAFGADQFDGQHPEESISKSSFFNWWYFGISLATFATVNILNYV 241
           SLYLV + QGGHKPCVQAFGADQFD   P+E IS+ SFFNWW+  +S     ++ ++ YV
Sbjct: 187 SLYLVAIGQGGHKPCVQAFGADQFDVGDPKERISRGSFFNWWFLSLSAGITLSIIVVVYV 246

Query: 242 QDNLSWSLGFGIPCIAMVLALVVFFLGTRTYRFSNRGDEE---NPFVRIGRVFITAVKNW 301
           QDN++W+LGFGIPC+ MV+AL +F  G +TYR+  RGD E   N F RIGRVF+ A KN 
Sbjct: 247 QDNVNWALGFGIPCLFMVMALALFLFGRKTYRYP-RGDREGKNNAFARIGRVFLVAFKNR 306

Query: 302 RVNSS-----EMAREEEIRGLLPHHSSKQLRFLDKALIAPNSLKEDGRACSISEVEEAKA 361
           ++  +     E+   ++ +G        QL FL KAL+      E    CS  +VE+A A
Sbjct: 307 KLKLTHSGQLEVGSYKKCKG--------QLEFLAKALLPGEGGVE---PCSSRDVEDAMA 366

Query: 362 VLRLVPIWVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFGFEVPAASLQSFISLAIVISLM 421
           ++RL+PIW+T +   I ++Q +TFFTKQGVT+DR I  GFE+P AS Q+ I L+I IS+ 
Sbjct: 367 LVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKILPGFEIPPASFQALIGLSIFISVP 426

Query: 422 IYDRILIPIARNFTGKPSGITTLQRIGFGMLLSAISMVIAALVEIKRLKTAQEYGLVDLP 481
            Y+R+ +P+AR  T KPSGIT LQRIG GM+LS+++MV+AALVE+KRL+TA+E+GLVD P
Sbjct: 427 TYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLNMVVAALVEMKRLETAKEHGLVDRP 486

Query: 482 KATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQVPSGLRSIGLSLYLSIFGIGNFLS 541
            AT+P+SIWW VPQY+L G+ D F++VG QEFFYDQVP+ LRSIGL+L LS  G+ +FLS
Sbjct: 487 DATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQVPTELRSIGLALSLSAMGLASFLS 546

Query: 542 SFLISAIEKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGLSALGLAAFLFSTRTYIYNK 592
            FLI+ I   T  NG  SWF+ NLN+AH+DYFYWLL   +A+G  AFL  +R Y+Y +
Sbjct: 547 GFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLLAAFTAIGFLAFLLLSRLYVYRR 560

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008437003.10.0e+0094.45PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo] >XP_008437004.1 PRE... [more]
XP_031741607.10.0e+0093.27protein NRT1/ PTR FAMILY 5.10 isoform X2 [Cucumis sativus][more]
XP_031741606.11.0e-30290.91protein NRT1/ PTR FAMILY 5.10 isoform X1 [Cucumis sativus] >KAE8647915.1 hypothe... [more]
XP_038877113.11.1e-28285.02protein NRT1/ PTR FAMILY 5.10 [Benincasa hispida] >XP_038877114.1 protein NRT1/ ... [more]
XP_022973964.18.0e-27683.00protein NRT1/ PTR FAMILY 5.10-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q0WP015.5e-20362.44Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV... [more]
Q0WSZ61.9e-17152.28Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV... [more]
Q9C7U11.9e-17154.58Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV... [more]
Q8VZE27.2e-17152.36Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV... [more]
Q9SK967.0e-16651.34Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A1S3ATI30.0e+0094.45protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103482564 PE=3 ... [more]
A0A5A7TJ760.0e+0094.45Protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A6J1IG543.9e-27683.00protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111472583 P... [more]
A0A6J1EHW26.1e-27482.49protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432710... [more]
A0A6J1EC562.0e-27280.62protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432731... [more]
Match NameE-valueIdentityDescription
AT1G22540.13.9e-20462.44Major facilitator superfamily protein [more]
AT1G72140.11.3e-17254.58Major facilitator superfamily protein [more]
AT1G72125.11.3e-17252.28Major facilitator superfamily protein [more]
AT1G72120.15.1e-17252.36Major facilitator superfamily protein [more]
AT1G22550.15.0e-16751.34Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (DHL92) v4
Date Performed: 2022-08-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 5..594
e-value: 3.9E-192
score: 641.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 24..585
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 140..552
e-value: 2.6E-90
score: 303.1
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 16..114
coord: 140..592
NoneNo IPR availablePANTHERPTHR11654:SF510FACILITATOR SUPERFAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATEDcoord: 16..114
coord: 140..592
IPR044739NRT1/PTR familyCDDcd17417MFS_NPF5coord: 29..589
e-value: 0.0
score: 676.294

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C005483.1MELO3C005483.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042938 dipeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity