MC11g_new0473 (gene) Bitter gourd (Dali-11) v1

BLAST of MC11g_new0473 vs. NCBI nr
Match: XP_022153214.1 (uncharacterized protein LOC111020765 [Momordica charantia])

HSP 1 Score: 157 bits (398), Expect = 9.00e-45
Identity = 80/179 (44.69%), Postives = 114/179 (63.69%), Query Frame = 0

Query: 27  SAALPRDFYSKLWNSGSPKKVNVLSWILMFGKVNTAEIMQNH-----LLVFFC---VNSS 86
           +A++P++F++ LW + SP++VNV  WIL  GK+NTA+I+Q       LL  FC     S 
Sbjct: 21  NASMPKEFFAALWKTKSPRRVNVFCWILFQGKLNTADIIQKKSPSDALLPSFCCLCTKSG 80

Query: 87  ENQLHTFFFCPFAATCWSAFFGIFNLPWVMDKSVSNLVFQLLFGL-HMNPRATLLWVNGL 146
           E+  H FF C FA+ CW+  F  FN+ W  D    + V+QLL G  H++     LW+N +
Sbjct: 81  EDHDHLFFHCYFASKCWNLLFHQFNVDWCFDLKAGDNVYQLLHGPPHLSSSVRFLWLNVV 140

Query: 147 KALIYKLWFKRNQRTFEGLKRHPLECFHIVKFKASQWCALSNLFVNYSPSMIDSNWDAF 196
           KAL+ +LWF+RN R FE  +R   E F+  KFKAS WC+L + F+++SPSMI +NW AF
Sbjct: 141 KALLSELWFERNSRLFEEKRRLFDESFYSAKFKASLWCSLVDSFLHHSPSMIYANWGAF 199

BLAST of MC11g_new0473 vs. NCBI nr
Match: XP_038903695.1 (uncharacterized protein LOC120090219 [Benincasa hispida])

HSP 1 Score: 137 bits (345), Expect = 1.74e-35
Identity = 71/188 (37.77%), Postives = 104/188 (55.32%), Query Frame = 0

Query: 19  SLCNSKAPSAALPRDFYSKLWNSGSPKKVNVLSWILMFGKVNTAEIMQNH--------LL 78
           SL N   P + L +  +S +W + SP++VN+L WI++FG++N AE++Q           +
Sbjct: 128 SLKNHLTPFSPLEKPIFSTIWKTKSPRRVNILIWIMLFGQLNVAEVLQKKQPTPSLSPTV 187

Query: 79  VFFCVNSSENQLHTFFFCPFAATCWSAFFGIFNLPWVMDKSVSNLVFQLLFGLHMNPRAT 138
             FC++ SE  LH FF CP+++ CW+     FNLP  +     + VFQLL     +    
Sbjct: 188 CPFCLHHSEVSLHLFFTCPYSSWCWNKLLCFFNLPLTLCNDFKSNVFQLLARPTSHKSTR 247

Query: 139 LLWVNGLKALIYKLWFKRNQRTFEGLKRHPLECFHIVKFKASQWCALSNLFVNYSPSMID 198
           LLW N +KAL+  LWF+RNQR F        +     + +AS WC LS+ F  YS S  +
Sbjct: 248 LLWCNAVKALLADLWFERNQRIFYNKATSCQDRLEAARRQASSWCLLSDPFRAYSLSDFN 307

BLAST of MC11g_new0473 vs. NCBI nr
Match: TYK21876.1 (hypothetical protein E5676_scaffold494G00090 [Cucumis melo var. makuwa])

HSP 1 Score: 126 bits (316), Expect = 1.07e-30
Identity = 64/185 (34.59%), Postives = 99/185 (53.51%), Query Frame = 0

Query: 19  SLCNSKAPSAALPRDFYSKLWNSGSPKKVNVLSWILMFGKVNTAEIMQNHLLVF------ 78
           SL      ++ + +  +S +  SGSP+++N+L WI++F  VN++EI+Q    +       
Sbjct: 198 SLSTHLKTASPMDKRLFSAILQSGSPRRINILIWIMVFRFVNSSEILQKKSPINVSPSIC 257

Query: 79  -FCVNSSENQLHTFFFCPFAATCWSAFFGIFNLPWVMDKSVSNLVFQLLFGLHMNPRATL 138
             C+ +S+N  H F +CP ++  W   F +FNL W  D S+S  V QLL G ++     +
Sbjct: 258 PLCLKASKNLPHIFLYCPVSSFGWERIFSLFNLVWNFDSSLSASVIQLLSGSNLPKTPRI 317

Query: 139 LWVNGLKALIYKLWFKRNQRTFEGLKRHPLECFHIVKFKASQWCALSNLFVNYSPSMIDS 196
           +W    KAL+ ++W +RNQR F    R   E  H     A+ WC+L   FVNYS   I  
Sbjct: 318 IWEILSKALLIEIWIERNQRIFHDKARQRAEIMHAADLNAAAWCSLRKEFVNYSIQDICL 377

BLAST of MC11g_new0473 vs. NCBI nr
Match: KAA0035739.1 (hypothetical protein E6C27_scaffold403G00100 [Cucumis melo var. makuwa])

HSP 1 Score: 122 bits (305), Expect = 8.98e-29
Identity = 63/184 (34.24%), Postives = 98/184 (53.26%), Query Frame = 0

Query: 19  SLCNSKAPSAALPRDFYSKLWNSGSPKKVNVLSWILMFGKVNTAEIMQNHLLVF------ 78
           SL      ++ + +  +S +  SGSP+++N+L WI++F  V ++EI+Q    +       
Sbjct: 198 SLSTHLKTASPMDKRLFSAILQSGSPRRINILIWIMVFRFVISSEILQKKSPINVSPSIC 257

Query: 79  -FCVNSSENQLHTFFFCPFAATCWSAFFGIFNLPWVMDKSVSNLVFQLLFGLHMNPRATL 138
             C+ +S+N  H F +CP ++  W   F +FNL W  D S+S  V QLL G ++     +
Sbjct: 258 PLCLKASKNLPHIFLYCPVSSFGWERIFSLFNLVWNFDSSLSASVIQLLSGSNLPKTPRI 317

Query: 139 LWVNGLKALIYKLWFKRNQRTFEGLKRHPLECFHIVKFKASQWCALSNLFVNYSPSMIDS 195
           +W    KAL+ ++W +RNQR F    R   E  H     A+ WC+L   FVNYS   I  
Sbjct: 318 IWEILSKALLIEIWIERNQRIFHDKARQRAEIMHAADLNAAAWCSLRKEFVNYSIQDICL 377

BLAST of MC11g_new0473 vs. NCBI nr
Match: RVX11717.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 87.8 bits (216), Expect = 2.07e-17
Identity = 52/183 (28.42%), Postives = 82/183 (44.81%), Query Frame = 0

Query: 19  SLCNSKAPSAALPRDFYSKLWNSGSPKKVNVLSWILMFGKVNTAEIMQNHL--------L 78
           +L     P   LP  F   LW+S  P KV VL+W++  GKVNT + +Q            
Sbjct: 35  ALSRDSNPLMFLPAKF---LWSSKVPSKVKVLAWLVAHGKVNTNDKLQLRRPYKALCPQW 94

Query: 79  VFFCVNSSENQLHTFFFCPFAATCWSAFFGIFNLPWVMDKSVSNLVFQLLFGLHMNPRAT 138
              C  + E+  H F  CP     W   F +  + WV  +S+ +++     GL  + R  
Sbjct: 95  CILCKGNGESIDHLFLHCPVTIGLWHKLFNLVGVIWVPPRSIEDMLVISFKGLGNSVRGK 154

Query: 139 LLWVNGLKALIYKLWFKRNQRTFEGLKRHPLECFHIVKFKASQWCALSNLFVNYSPSMID 193
           +LW      LI+ +W +RN R FE   R     + +++F +S W + +  F     S++ 
Sbjct: 155 ILWQIACLTLIWMVWQERNNRIFEDKGRTEEVVWDLIRFYSSLWASCTEAFRGVPLSILQ 214

BLAST of MC11g_new0473 vs. ExPASy TrEMBL
Match: A0A6J1DIE2 (uncharacterized protein LOC111020765 OS=Momordica charantia OX=3673 GN=LOC111020765 PE=4 SV=1)

HSP 1 Score: 157 bits (398), Expect = 4.36e-45
Identity = 80/179 (44.69%), Postives = 114/179 (63.69%), Query Frame = 0

Query: 27  SAALPRDFYSKLWNSGSPKKVNVLSWILMFGKVNTAEIMQNH-----LLVFFC---VNSS 86
           +A++P++F++ LW + SP++VNV  WIL  GK+NTA+I+Q       LL  FC     S 
Sbjct: 21  NASMPKEFFAALWKTKSPRRVNVFCWILFQGKLNTADIIQKKSPSDALLPSFCCLCTKSG 80

Query: 87  ENQLHTFFFCPFAATCWSAFFGIFNLPWVMDKSVSNLVFQLLFGL-HMNPRATLLWVNGL 146
           E+  H FF C FA+ CW+  F  FN+ W  D    + V+QLL G  H++     LW+N +
Sbjct: 81  EDHDHLFFHCYFASKCWNLLFHQFNVDWCFDLKAGDNVYQLLHGPPHLSSSVRFLWLNVV 140

Query: 147 KALIYKLWFKRNQRTFEGLKRHPLECFHIVKFKASQWCALSNLFVNYSPSMIDSNWDAF 196
           KAL+ +LWF+RN R FE  +R   E F+  KFKAS WC+L + F+++SPSMI +NW AF
Sbjct: 141 KALLSELWFERNSRLFEEKRRLFDESFYSAKFKASLWCSLVDSFLHHSPSMIYANWGAF 199

BLAST of MC11g_new0473 vs. ExPASy TrEMBL
Match: A0A5D3DE60 (zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold494G00090 PE=4 SV=1)

HSP 1 Score: 126 bits (316), Expect = 5.17e-31
Identity = 64/185 (34.59%), Postives = 99/185 (53.51%), Query Frame = 0

Query: 19  SLCNSKAPSAALPRDFYSKLWNSGSPKKVNVLSWILMFGKVNTAEIMQNHLLVF------ 78
           SL      ++ + +  +S +  SGSP+++N+L WI++F  VN++EI+Q    +       
Sbjct: 198 SLSTHLKTASPMDKRLFSAILQSGSPRRINILIWIMVFRFVNSSEILQKKSPINVSPSIC 257

Query: 79  -FCVNSSENQLHTFFFCPFAATCWSAFFGIFNLPWVMDKSVSNLVFQLLFGLHMNPRATL 138
             C+ +S+N  H F +CP ++  W   F +FNL W  D S+S  V QLL G ++     +
Sbjct: 258 PLCLKASKNLPHIFLYCPVSSFGWERIFSLFNLVWNFDSSLSASVIQLLSGSNLPKTPRI 317

Query: 139 LWVNGLKALIYKLWFKRNQRTFEGLKRHPLECFHIVKFKASQWCALSNLFVNYSPSMIDS 196
           +W    KAL+ ++W +RNQR F    R   E  H     A+ WC+L   FVNYS   I  
Sbjct: 318 IWEILSKALLIEIWIERNQRIFHDKARQRAEIMHAADLNAAAWCSLRKEFVNYSIQDICL 377

BLAST of MC11g_new0473 vs. ExPASy TrEMBL
Match: A0A5A7T2Y0 (zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold403G00100 PE=4 SV=1)

HSP 1 Score: 122 bits (305), Expect = 4.35e-29
Identity = 63/184 (34.24%), Postives = 98/184 (53.26%), Query Frame = 0

Query: 19  SLCNSKAPSAALPRDFYSKLWNSGSPKKVNVLSWILMFGKVNTAEIMQNHLLVF------ 78
           SL      ++ + +  +S +  SGSP+++N+L WI++F  V ++EI+Q    +       
Sbjct: 198 SLSTHLKTASPMDKRLFSAILQSGSPRRINILIWIMVFRFVISSEILQKKSPINVSPSIC 257

Query: 79  -FCVNSSENQLHTFFFCPFAATCWSAFFGIFNLPWVMDKSVSNLVFQLLFGLHMNPRATL 138
             C+ +S+N  H F +CP ++  W   F +FNL W  D S+S  V QLL G ++     +
Sbjct: 258 PLCLKASKNLPHIFLYCPVSSFGWERIFSLFNLVWNFDSSLSASVIQLLSGSNLPKTPRI 317

Query: 139 LWVNGLKALIYKLWFKRNQRTFEGLKRHPLECFHIVKFKASQWCALSNLFVNYSPSMIDS 195
           +W    KAL+ ++W +RNQR F    R   E  H     A+ WC+L   FVNYS   I  
Sbjct: 318 IWEILSKALLIEIWIERNQRIFHDKARQRAEIMHAADLNAAAWCSLRKEFVNYSIQDICL 377

BLAST of MC11g_new0473 vs. ExPASy TrEMBL
Match: A0A438JRY4 (Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDp000001_837 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.00e-17
Identity = 52/183 (28.42%), Postives = 82/183 (44.81%), Query Frame = 0

Query: 19  SLCNSKAPSAALPRDFYSKLWNSGSPKKVNVLSWILMFGKVNTAEIMQNHL--------L 78
           +L     P   LP  F   LW+S  P KV VL+W++  GKVNT + +Q            
Sbjct: 35  ALSRDSNPLMFLPAKF---LWSSKVPSKVKVLAWLVAHGKVNTNDKLQLRRPYKALCPQW 94

Query: 79  VFFCVNSSENQLHTFFFCPFAATCWSAFFGIFNLPWVMDKSVSNLVFQLLFGLHMNPRAT 138
              C  + E+  H F  CP     W   F +  + WV  +S+ +++     GL  + R  
Sbjct: 95  CILCKGNGESIDHLFLHCPVTIGLWHKLFNLVGVIWVPPRSIEDMLVISFKGLGNSVRGK 154

Query: 139 LLWVNGLKALIYKLWFKRNQRTFEGLKRHPLECFHIVKFKASQWCALSNLFVNYSPSMID 193
           +LW      LI+ +W +RN R FE   R     + +++F +S W + +  F     S++ 
Sbjct: 155 ILWQIACLTLIWMVWQERNNRIFEDKGRTEEVVWDLIRFYSSLWASCTEAFRGVPLSILQ 214

BLAST of MC11g_new0473 vs. ExPASy TrEMBL
Match: A0A438F6T1 (zf-RVT domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_085247 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 2.43e-17
Identity = 56/197 (28.43%), Postives = 88/197 (44.67%), Query Frame = 0

Query: 5   QQLCEKIGDLGSRCSLCNSKAPSAALPRDFYSKLWNSGSPKKVNVLSWILMFGKVNTAEI 64
           Q+L   I  +    S  NS+A S +    F   LW+S  P KV  L+W++  GKVNT + 
Sbjct: 118 QRLMSSISSVFFSPSSSNSRAWSLSSSAKF---LWSSKVPSKVKALAWLVAHGKVNTNDK 177

Query: 65  MQ--------NHLLVFFCVNSSENQLHTFFFCPFAATCWSAFFGIFNLPWVMDKSVSNLV 124
           +Q        +      C  + E+  H F  CP     W   F +  L WV  +S+ +++
Sbjct: 178 LQLRRPYKVLSPQWCILCKGNGESIDHLFLHCPITIGLWHRLFNLVGLVWVPPRSLEDML 237

Query: 125 FQLLFGLHMNPRATLLWVNGLKALIYKLWFKRNQRTFEGLKRHPLECFHIVKFKASQWCA 184
                GL  + R   LW      LI+ +W +RN R FE   R     + +++F +S W +
Sbjct: 238 VITFKGLGNSLRGKTLWQIACLTLIWMVWQERNNRIFEDKGRTEEMVWDLIRFYSSLWAS 297

Query: 185 LSNLFVNYSPSMIDSNW 193
            +  F     S++  NW
Sbjct: 298 CTEAFRGVPLSVLQLNW 311