MC11g_new0429 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC11g_new0429
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDNA-directed RNA polymerase III subunit RPC9
LocationMC11: 17389775 .. 17392818 (-)
RNA-Seq ExpressionMC11g_new0429
SyntenyMC11g_new0429
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGTACCCAACAATCAATTTACAGCCAAATTCTGTTCTGGCTTATTATAATTGCTAGTAGATCTAATGGATATTGTTCAATGATTAATGCTTGATGTTTGGGTAAAAGCATTGATTGATTATGTTTAATATGAAGCATAATGGCATGGTCATGGTTTAGTATATTGAAATGAACTTGTTTAATAGTATTTGCTTTTGTGGGTTATGTAGCATGTTGGATTATTGAGAGATAAATGGTGCATTTTCATGACTTAGAAAACATATTATAGGGTGCATATGTGAGCTGTTATGAAGTAAATGGGTGAATATTAGGGTCTAGGTCGATTTATCACTATTGGGTATTAAGGTCTCGGGTTCAAATGGTCAATGGTTGAGGCTTTGAGTCTAGCTGGAAGGGGCACTATTGGGACCTAAGGTATAAATTGTCATTGGTTGTGGGGGTTTGAGATGTGAGTTATGATATTGAAAAAATTGATGGGTGAGCAGGACCTTGGGTGAAATAGTAAGGGCCAATACATAACTATTGGGTATCGAGATCTCGAGTAAAAATGGACGGAGGTCAATACGTCAGTCTTGGATAAGATGAGTGTCAATGTCTCAAATAAAAAGGGTGAGGGTAGATGCGATGACTTTAGCTGAGAGGCAAATATTAGGATCTTGGATTTGAATGGTCAAGGGTCGATGTGGTTCGAGGTGTTGAATAAAAATGGTCAGGGTGGTCGAACGCTAGTTCTTTAGAGAAGTGTCGAGACCATGAATAAAAATTTTCAGGATCGATGTGACTCGAAAGATTAAGGCGGGGAGGAATAAGAAGAAATTGAAAAAAGTTTGAAAGAGTTAGAGACTCTAGTGAAAATTATGACGAGAGAATCTATCATTGAGTTTGTTGACAAGTCCAAAAAGTATGATCTCGCGAAAGCTGAAATTCTCAATATCATTAACTTGCGGCCAATTACTCCGGTTGAGATATTTCCGGTATTTTCCCCCTTTCTTTCTCCCTTCCATGACAGTAATTCTTCACTTTATTTTCTTCGTTTATTTTACATATTAATTCTATGCTGTTCCACCATGTTTATATTTTGTGTGTGATCTCTGTTCTAAACTCATATTTCCTTTTCCCATGTGGATGAGGCCATTTGTTTTTCATTGGTGCCTTTAGATTTTTAGAAGAGTGTTTACATGATATAGGAAGTCAAATATCAAATGTTAATTTGCAGTTTTATTGTTTTGTAATAGAATTATGTAGTCTTTGTGGCATTTCCCCTTTTTTGTTGACATTCCAAAAGCCCTAAATTCTCTAGGTATGGAGCACATTTGTCTGGTTATTAAGAACTTGGTGGAGTGGATGTCATAAATTGTTGTTGAGGTTCAACTTGGGTATTTGGCTGGAGAGGAACACTAGAATTTTCAGGGGTGGAAAAATTTGTGGACTCTGTTTGGGATCTTATTAGGTTTAGTACTTCTTTGTGGGGTTCGGTCTCTAAGGCGTTTTGTAATTATCAGCTAGGTGGTATTCTTTTGGATTGGGCTATTTTCGCTCGTAGGGCTGTTTTTTTTGTATGCTCTTCTTGTTCTTTTCATTTTTCTAAGTGAAAACGTGATTTCTTATTAAAAAAAAATATTAAGGTTCAACTGTAGTCATCTTAATAAGTTAAACTTAAAGCTATGAGACTTGGGCTTTCGGAGAGGCTTTTTTGGCATAGAGGTAGACGGTTGAGGCTAGTGCAGAAGCTGGATACTACGAAATTAGGGGAGGGCGTATTCCTTTCAAATAATGTTTATTAGTTTGGCTTTCAGCTTGGGGCAGTTTTCATCAGCTTCAAAAAAAGCAGACTTAGCAGTTTCATCACCAACAAGAATACACCTCCTCCTCTGTTGACTCACACACATTGGACTTTGACATGGTCGATGTATGGGCGATGAGAAAGGGGTTGTTCTGAAGCATATGTACTCCTTGTACATCTTGGTTGTAAAAAATGATTGGAACGGTAGGCATTTTAGAGTACGAGATATGTACTCTGTGCCTTGCCTCTCTGACCAAGGCCTTCCGTTTATGGAACTGTGCTTCGAGAGAAATTTTGTGGAATCTTTGGCTTGAAAAAGATCTCATTTGAAGGTAAGTCGTCATCTTTTGATTCGTTCCGTAACAGTGTACATACCTTTGTCTCTTGGTGGTGCCATGAAAATAGAAAATTCTTTTGTGACCTCTCTATATGGATATGTAGGATTGGAGAGCCATTCTTTTGGGGGTTCATCCCCGCCCTTAAGCTGTTCTTTTTGTTCTTTTCTTTGCTGAATGACGTATTTTCTTATCCAAAACCATTAAGGTGTAACATTAACTGTGATTCATTACTTTTGTTTTTAGATTATAGAGGACAGTGATGTTCGCATGGGAGATGATGGAATTCAAGAGCTTACAGAGATGGTGGAGCAGGTGTTTCCTCCGCCTCAAACTCAACCAAAGTAAGGCAAAGAGGCCGAAGGCATGGAGGAACAGGAAGTCGGAGAGCCTGCCGATGGAGAACCAATGGAAGAAACTACTTGAACCCAGTTCGAAAATTAGATGATATATCGGTACATAACAATTCTCAGCGTTCAGAAGAGCTTTCACAAAGGACTTGGTTCTGTTCTGCATCCTTCAAGCATTAAGTACTCATAATGTGCAGATATGGGTTTTGAGATGCAAGATGAAACTGAGAGTGACAGTTCCCAAAAAAAATAAAATGGGAAATCAAAGTCAAAGAGAGCTTTTGGGTGTTAAAGTTAAACAATGGAAATAGCTAGGCTTCTTTCTAAATGTAATCTAATATTTTTAGGTAGGGAAGAAAGATGAAGCCGAGATTTTTGTTTCCCTGGGTTACTCCCATTTCCTCAAAGCTAGCAGAGGCCACACACATAGGCAGTAGTATGTATAAGTTTGCAAAAGGTCGTGACTCGGAATGGTCATGAATTTGAATCTTTACTTCATATGTTCTACTGGAAAAATCAATATGCATCATCGATTAACTTAACTTACTCATCGATCAATTTTAACAATTCATTCAT

mRNA sequence

ATGACGAGAGAATCTATCATTGAGTTTGTTGACAAGTCCAAAAAGTATGATCTCGCGAAAGCTGAAATTCTCAATATCATTAACTTGCGGCCAATTACTCCGGTTGAGATATTTCCGCTTGGGGCAGTTTTCATCAGCTTCAAAAAAAGCAGACTTAGCAGTTTCATCACCAACAAGAATACACCTCCTCCTCTGTTGACTCACACACATTGGACTTTGACATGGTCGATGTATGGGCGATGA

Coding sequence (CDS)

ATGACGAGAGAATCTATCATTGAGTTTGTTGACAAGTCCAAAAAGTATGATCTCGCGAAAGCTGAAATTCTCAATATCATTAACTTGCGGCCAATTACTCCGGTTGAGATATTTCCGCTTGGGGCAGTTTTCATCAGCTTCAAAAAAAGCAGACTTAGCAGTTTCATCACCAACAAGAATACACCTCCTCCTCTGTTGACTCACACACATTGGACTTTGACATGGTCGATGTATGGGCGATGA

Protein sequence

MTRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPLGAVFISFKKSRLSSFITNKNTPPPLLTHTHWTLTWSMYGR
Homology
BLAST of MC11g_new0429 vs. NCBI nr
Match: XP_023000556.1 (DNA-directed RNA polymerase III subunit RPC9-like [Cucurbita maxima])

HSP 1 Score: 75.5 bits (184), Expect = 5.47e-15
Identity = 36/39 (92.31%), Postives = 39/39 (100.00%), Query Frame = 0

Query: 2  TRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPL 40
          TRE+IIEFVDKSKKYDLAKAEILNI+NLRPITPVEIFP+
Sbjct: 56 TREAIIEFVDKSKKYDLAKAEILNIVNLRPITPVEIFPI 94

BLAST of MC11g_new0429 vs. NCBI nr
Match: KAG7026218.1 (DNA-directed RNA polymerase III subunit RPC9 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 75.5 bits (184), Expect = 5.73e-15
Identity = 36/39 (92.31%), Postives = 39/39 (100.00%), Query Frame = 0

Query: 2  TRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPL 40
          TRE+IIEFVDKSKKYDLAKAEILNI+NLRPITPVEIFP+
Sbjct: 56 TREAIIEFVDKSKKYDLAKAEILNIVNLRPITPVEIFPI 94

BLAST of MC11g_new0429 vs. NCBI nr
Match: XP_022930221.1 (DNA-directed RNA polymerase III subunit RPC9-like [Cucurbita moschata])

HSP 1 Score: 75.5 bits (184), Expect = 5.73e-15
Identity = 36/39 (92.31%), Postives = 39/39 (100.00%), Query Frame = 0

Query: 2  TRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPL 40
          TRE+IIEFVDKSKKYDLAKAEILNI+NLRPITPVEIFP+
Sbjct: 56 TREAIIEFVDKSKKYDLAKAEILNIVNLRPITPVEIFPI 94

BLAST of MC11g_new0429 vs. NCBI nr
Match: XP_023006759.1 (uncharacterized protein LOC111499396 [Cucurbita maxima] >XP_023006760.1 uncharacterized protein LOC111499396 [Cucurbita maxima])

HSP 1 Score: 74.3 bits (181), Expect = 1.69e-14
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 0

Query: 2  TRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPL 40
          TRE+IIEFVDKSKKYDLAKAE+LNI+NLRPITPVEIFP+
Sbjct: 56 TREAIIEFVDKSKKYDLAKAELLNILNLRPITPVEIFPI 94

BLAST of MC11g_new0429 vs. NCBI nr
Match: XP_023548749.1 (uncharacterized protein LOC111807313 [Cucurbita pepo subsp. pepo] >KAG6574960.1 DNA-directed RNA polymerase III subunit RPC9, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013528.1 DNA-directed RNA polymerase III subunit RPC9 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 74.3 bits (181), Expect = 1.69e-14
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 0

Query: 2  TRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPL 40
          TRE+IIEFVDKSKKYDLAKAE+LNI+NLRPITPVEIFP+
Sbjct: 56 TREAIIEFVDKSKKYDLAKAELLNILNLRPITPVEIFPI 94

BLAST of MC11g_new0429 vs. ExPASy TrEMBL
Match: A0A6J1KG58 (DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita maxima OX=3661 GN=LOC111494797 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.65e-15
Identity = 36/39 (92.31%), Postives = 39/39 (100.00%), Query Frame = 0

Query: 2  TRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPL 40
          TRE+IIEFVDKSKKYDLAKAEILNI+NLRPITPVEIFP+
Sbjct: 56 TREAIIEFVDKSKKYDLAKAEILNIVNLRPITPVEIFPI 94

BLAST of MC11g_new0429 vs. ExPASy TrEMBL
Match: A0A6J1EQC1 (DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita moschata OX=3662 GN=LOC111436740 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.77e-15
Identity = 36/39 (92.31%), Postives = 39/39 (100.00%), Query Frame = 0

Query: 2  TRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPL 40
          TRE+IIEFVDKSKKYDLAKAEILNI+NLRPITPVEIFP+
Sbjct: 56 TREAIIEFVDKSKKYDLAKAEILNIVNLRPITPVEIFPI 94

BLAST of MC11g_new0429 vs. ExPASy TrEMBL
Match: A0A6J1L324 (DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita maxima OX=3661 GN=LOC111499396 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 8.18e-15
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 0

Query: 2  TRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPL 40
          TRE+IIEFVDKSKKYDLAKAE+LNI+NLRPITPVEIFP+
Sbjct: 56 TREAIIEFVDKSKKYDLAKAELLNILNLRPITPVEIFPI 94

BLAST of MC11g_new0429 vs. ExPASy TrEMBL
Match: A0A6J1H2X6 (DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita moschata OX=3662 GN=LOC111459926 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 8.18e-15
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 0

Query: 2  TRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPL 40
          TRE+IIEFVDKSKKYDLAKAE+LNI+NLRPITPVEIFP+
Sbjct: 56 TREAIIEFVDKSKKYDLAKAELLNILNLRPITPVEIFPI 94

BLAST of MC11g_new0429 vs. ExPASy TrEMBL
Match: A0A6J1CCS4 (DNA-directed RNA polymerase III subunit RPC9 OS=Momordica charantia OX=3673 GN=LOC111009544 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 2.30e-14
Identity = 36/38 (94.74%), Postives = 37/38 (97.37%), Query Frame = 0

Query: 3  RESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPL 40
          RESIIEFVDKSKKYDLAKAEILNIINLRP TPVEIFP+
Sbjct: 57 RESIIEFVDKSKKYDLAKAEILNIINLRPTTPVEIFPI 94

BLAST of MC11g_new0429 vs. TAIR 10
Match: AT5G62950.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 52.0 bits (123), Expect = 2.6e-07
Identity = 26/39 (66.67%), Postives = 31/39 (79.49%), Query Frame = 0

Query: 2  TRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPL 41
          TRESI +F DK K + LAKAEILNIINLRP + VE+ P+
Sbjct: 56 TRESINKFADKCKDFKLAKAEILNIINLRPSSIVELLPI 94

BLAST of MC11g_new0429 vs. TAIR 10
Match: AT5G62950.3 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 52.0 bits (123), Expect = 2.6e-07
Identity = 26/39 (66.67%), Postives = 31/39 (79.49%), Query Frame = 0

Query: 2  TRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPL 41
          TRESI +F DK K + LAKAEILNIINLRP + VE+ P+
Sbjct: 56 TRESINKFADKCKDFKLAKAEILNIINLRPSSIVELLPI 94

BLAST of MC11g_new0429 vs. TAIR 10
Match: AT5G62950.2 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 51.6 bits (122), Expect = 3.4e-07
Identity = 26/38 (68.42%), Postives = 30/38 (78.95%), Query Frame = 0

Query: 2  TRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFP 40
          TRESI +F DK K + LAKAEILNIINLRP + VE+ P
Sbjct: 56 TRESINKFADKCKDFKLAKAEILNIINLRPSSIVELLP 93

BLAST of MC11g_new0429 vs. TAIR 10
Match: AT3G28956.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 45.4 bits (106), Expect = 2.4e-05
Identity = 23/39 (58.97%), Postives = 29/39 (74.36%), Query Frame = 0

Query: 2  TRESIIEFVDKSKKYDLAKAEILNIINLRPITPVEIFPL 41
          TRES+ +  DK K + LAKAEILNIINL P + VE+ P+
Sbjct: 53 TRESVNKSADKCKDFKLAKAEILNIINLWPSSIVELLPI 91

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_023000556.15.47e-1592.31DNA-directed RNA polymerase III subunit RPC9-like [Cucurbita maxima][more]
KAG7026218.15.73e-1592.31DNA-directed RNA polymerase III subunit RPC9 [Cucurbita argyrosperma subsp. argy... [more]
XP_022930221.15.73e-1592.31DNA-directed RNA polymerase III subunit RPC9-like [Cucurbita moschata][more]
XP_023006759.11.69e-1489.74uncharacterized protein LOC111499396 [Cucurbita maxima] >XP_023006760.1 uncharac... [more]
XP_023548749.11.69e-1489.74uncharacterized protein LOC111807313 [Cucurbita pepo subsp. pepo] >KAG6574960.1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1KG582.65e-1592.31DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1EQC12.77e-1592.31DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1L3248.18e-1589.74DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1H2X68.18e-1589.74DNA-directed RNA polymerase III subunit RPC9 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1CCS42.30e-1494.74DNA-directed RNA polymerase III subunit RPC9 OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G62950.12.6e-0766.67RNA polymerase II, Rpb4, core protein [more]
AT5G62950.32.6e-0766.67RNA polymerase II, Rpb4, core protein [more]
AT5G62950.23.4e-0768.42RNA polymerase II, Rpb4, core protein [more]
AT3G28956.12.4e-0558.97RNA polymerase II, Rpb4, core protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038324Rpb4/RPC9 superfamilyGENE3D1.20.1250.40coord: 1..54
e-value: 1.9E-9
score: 39.6
IPR010997HRDC-like superfamilySUPERFAMILY47819HRDC-likecoord: 2..39

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC11g_new0429.1MC11g_new0429.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006384 transcription initiation from RNA polymerase III promoter
biological_process GO:0044237 cellular metabolic process
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0000166 nucleotide binding