MC11g_new0172 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC11g_new0172
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Carbohydrate-binding X8 domain superfamily protein
Location	MC11: 6646195 .. 6647544 (-)
RNA-Seq Expression	MC11g_new0172
Synteny	MC11g_new0172

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATGATCAAAAGTGTTATTTAACATTTTTTATTTTTAATTTTTAAGAAAAGGAGAGTTATTAAAAGCCTCCATGAACGTGCACCTCGATCCGATGGTCAAGAAAAGTAAATTTTTTGTGAAAATAAAAGAAAGTCGAAGAGAAAGTGGGAGAAAAAGAAGAAGCTGCAACTGCAAGGAGGAAAAATCCACAAAAAGCGTCGCCATCATGTGCCCGTCCTTTGAGCCTCCTTCAATCTCTTCGCTTTCCGTTTTTCTATTTCTTTTCTCCCGAGAAGATTTCGATTCCCCCACCTACCCTAACGGCTCCGCATCGCATTACTACTGGAATTTGTAGCCGTTACCATGGACACCCGACGATTTTCCTGCTATAAATCCTCCTCCATCCTCTGATTTTGGCGATCACCAAATCCTCCGCCCTCGATTTCACGTATCTGTAGCTCTTTCTTCGATTTCAGATCTGGTTGTTGCAGAAATCAGGTACGATTAATGGCGGCGGCGGCGGCTCTCTGCTTTTCGCTTCTGTTCCTCTCTTTCCTCTCAGGTGCGTTAATTTGCGCAGTTTTCGATTCGAGGAATCAACTGAATCTGAATGAATCATTGCTGAATTTCCTTCTCTCTACTTCCGATTTGTGTTTCAGAAGGAGATTTGCTGGCGGTGAATGGAGAGGTAATTCTAATTCTCGTCGAATTGCAGATCAGAGCATGAACAGAATTAGGTTGAAAACTGAATTGATTTTAATTTTTCCCCCTCCATGCGATATGAAATTTGCAGAAGACGTGGTGCGTCGCGAGGCCGTCGTCGGATAAAGCTACGCTACTGGCGAATATCAATTACGCTTGCTCGCAGGTGGATTGCAGGATAATGCAGAAGGGATGCCCCTGCTTTTATCCTGATAATCTGATCAACGGCGCCTCCGTAGCCATGAATCTCTATTATCATTCCAGAGGGAAGAATCAGTGGAACTGTGATTTCAGAGGCTCTGCTCTCATTGTCATCACTGATCCAAGTGAGTCCGCTTCTTTGATTGATCTTTGGAATTTTTGTTCCTGTTCGTTCTTCATCTTTTTTGATTTGTGGTTCTAATTTTGCAGGTTATGGAAACTGCATTTACCCTTGAAATTATATATAGATTATAAAGTTTGCTCTGATTTTTATTGAAGATTCGATGTAAGTGAGAAAGAGATAGGATACTGAATTAATGAAAGCTACTACATCAGAAATTTGGGCTTTAGCATCAATTATTTATTGTTAACTTTTTTTTTTTTAAAAATTCAACAATAACATCTAATATTAATGTGCCTTGTCCCCTGAGTTATAATTTTTCTTTTTAATCTGTTTAGATCGTGT

mRNA sequence

ATGGCGGCGGCGGCGGCTCTCTGCTTTTCGCTTCTGTTCCTCTCTTTCCTCTCAGAAGGAGATTTGCTGGCGGTGAATGGAGAGAAGACGTGGTGCGTCGCGAGGCCGTCGTCGGATAAAGCTACGCTACTGGCGAATATCAATTACGCTTGCTCGCAGGTGGATTGCAGGATAATGCAGAAGGGATGCCCCTGCTTTTATCCTGATAATCTGATCAACGGCGCCTCCGTAGCCATGAATCTCTATTATCATTCCAGAGGGAAGAATCAGTGGAACTGTGATTTCAGAGGCTCTGCTCTCATTGTCATCACTGATCCAAGTTATGGAAACTGCATTTACCCTTGA

Coding sequence (CDS)

ATGGCGGCGGCGGCGGCTCTCTGCTTTTCGCTTCTGTTCCTCTCTTTCCTCTCAGAAGGAGATTTGCTGGCGGTGAATGGAGAGAAGACGTGGTGCGTCGCGAGGCCGTCGTCGGATAAAGCTACGCTACTGGCGAATATCAATTACGCTTGCTCGCAGGTGGATTGCAGGATAATGCAGAAGGGATGCCCCTGCTTTTATCCTGATAATCTGATCAACGGCGCCTCCGTAGCCATGAATCTCTATTATCATTCCAGAGGGAAGAATCAGTGGAACTGTGATTTCAGAGGCTCTGCTCTCATTGTCATCACTGATCCAAGTTATGGAAACTGCATTTACCCTTGA

Protein sequence

MAAAAALCFSLLFLSFLSEGDLLAVNGEKTWCVARPSSDKATLLANINYACSQVDCRIMQKGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP

Homology

[Close]

BLAST of MC11g_new0172 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 3.1e-19
Identity = 41/86 (47.67%), Postives = 53/86 (61.63%), Query Frame = 0


Query: 30  TWCVARPSSDKATLLANINYACSQ-VDCRIMQKGCPCFYPDNLINGASVAMNLYYHSRGK 89
           +WCV +P      L  NINYAC Q +DC  +Q G  CF P+ +   A+  MNLYY S G+
Sbjct: 371 SWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQSAGR 430

Query: 90  NQWNCDFRGSALIVITDPSYGNCIYP 115
           N WNCDF  +A +  T+PSYG C +P
Sbjct: 431 NSWNCDFSQTATLTNTNPSYGACNFP 456

[Close]

BLAST of MC11g_new0172 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.2e-18
Identity = 40/90 (44.44%), Postives = 54/90 (60.00%), Query Frame = 0


Query: 24  AVNGEKTWCVARPSSDKATLLANINYACSQVDCRIMQKGCPCFYPDNLINGASVAMNLYY 83
           A +G   WCVA+  ++   L  NINYAC  VDC+ +Q G  CF P++L   AS  MN YY
Sbjct: 370 APSGGGKWCVAKDGANGTDLQNNINYACGFVDCKPIQSGGACFSPNSLQAHASYVMNAYY 429

Query: 84  HSRGKNQWNCDFRGSALIVITDPSYGNCIY 114
            + G     CDF+G+ ++  +DPSYG C Y
Sbjct: 430 QANGHTDLACDFKGTGIVTSSDPSYGGCKY 459

[Close]

BLAST of MC11g_new0172 vs. ExPASy Swiss-Prot
Match: Q84V39 (Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.9e-16
Identity = 38/89 (42.70%), Postives = 55/89 (61.80%), Query Frame = 0


Query: 27  GEKTWCVARPSSDKATLLANINYACSQ--VDCRIMQKGCPCFYPDNLINGASVAMNLYYH 86
           G K WCV +  +  A L +NI+Y CSQ  +DC  +Q    CF P+ +   AS AMN +Y 
Sbjct: 33  GNKKWCVPKAEATDAQLQSNIDYVCSQSGMDCGPIQANGACFNPNTVRAHASYAMNSWYQ 92

Query: 87  SRGKNQWNCDFRGSALIVITDPSYGNCIY 114
           S+G+N ++CDF G+  I  +DPS G+C +
Sbjct: 93  SKGRNDFDCDFSGTGAITSSDPSNGSCSF 121

[Close]

BLAST of MC11g_new0172 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 4.2e-16
Identity = 36/89 (40.45%), Postives = 51/89 (57.30%), Query Frame = 0


Query: 27  GEKTWCVARPSSDKATLLANINYAC--SQVDCRIMQKGCPCFYPDNLINGASVAMNLYYH 86
           G K WC+A   +    L   +++AC    VDC  +Q   PCF PD +++ AS A N YY 
Sbjct: 387 GTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYYQ 446

Query: 87  SRGKNQWNCDFRGSALIVITDPSYGNCIY 114
             G +  +C F G+++ V  DPSYGNC+Y
Sbjct: 447 QSGASSIDCSFNGASVEVDKDPSYGNCLY 475

[Close]

BLAST of MC11g_new0172 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 83.6 bits (205), Expect = 1.6e-15
Identity = 36/88 (40.91%), Postives = 55/88 (62.50%), Query Frame = 0


Query: 29  KTWCVARPSSDKATLLANINYAC--SQVDCRIMQKGCPCFYPDNLINGASVAMNLYYHSR 88
           +T+C+A    D  TL A +++AC   + +C  +Q G  C+ P+N+   AS A N YY   
Sbjct: 379 QTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKE 438

Query: 89  GKNQWNCDFRGSALIVITDPSYGNCIYP 115
           G+   +CDF+G A+I  TDPS+G+CI+P
Sbjct: 439 GRASGSCDFKGVAMITTTDPSHGSCIFP 466

[Close]

BLAST of MC11g_new0172 vs. NCBI nr
Match: XP_022137818.1 (glucan endo-1,3-beta-D-glucosidase [Momordica charantia])

HSP 1 Score: 241 bits (615), Expect = 1.90e-80
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MAAAAALCFSLLFLSFLSEGDLLAVNGEKTWCVARPSSDKATLLANINYACSQVDCRIMQ 60
           MAAAAALCFSLLFLSFLSEGDLLAVNGEKTWCVARPSSDKATLLANINYACSQVDCRIMQ
Sbjct: 1   MAAAAALCFSLLFLSFLSEGDLLAVNGEKTWCVARPSSDKATLLANINYACSQVDCRIMQ 60

Query: 61  KGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114
           KGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP
Sbjct: 61  KGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114

[Close]

BLAST of MC11g_new0172 vs. NCBI nr
Match: XP_022941530.1 (major pollen allergen Ole e 10-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 202 bits (514), Expect = 4.56e-65
Identity = 97/113 (85.84%), Postives = 102/113 (90.27%), Query Frame = 0


Query: 2   AAAAALCFSLLFLSFLSEGDLLAVNGEKTWCVARPSSDKATLLANINYACSQVDCRIMQK 61
           AAA AL F LLFLS    GDLLAVNG +TWCVARPSSD ATLLANINYACSQVDCRIMQK
Sbjct: 4   AAAVALSFLLLFLS----GDLLAVNGYQTWCVARPSSDHATLLANINYACSQVDCRIMQK 63

Query: 62  GCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114
           GCPCFYPDNLIN AS+AMNLYY+S+GKNQWNCDFRGS LIVITDPSYGNC+YP
Sbjct: 64  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 112

[Close]

BLAST of MC11g_new0172 vs. NCBI nr
Match: XP_022941529.1 (glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata] >KAG7037466.1 Glucan endo-1,3-beta-D-glucosidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 201 bits (510), Expect = 1.92e-64
Identity = 98/114 (85.96%), Postives = 103/114 (90.35%), Query Frame = 0


Query: 2   AAAAALCFSLLFLSFLSEGDLLAVNG-EKTWCVARPSSDKATLLANINYACSQVDCRIMQ 61
           AAA AL F LLFLS    GDLLAVNG +KTWCVARPSSD ATLLANINYACSQVDCRIMQ
Sbjct: 4   AAAVALSFLLLFLS----GDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQ 63

Query: 62  KGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114
           KGCPCFYPDNLIN AS+AMNLYY+S+GKNQWNCDFRGS LIVITDPSYGNC+YP
Sbjct: 64  KGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 113

[Close]

BLAST of MC11g_new0172 vs. NCBI nr
Match: KAG6607951.1 (Glucan endo-1,3-beta-D-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 200 bits (508), Expect = 3.88e-64
Identity = 97/114 (85.09%), Postives = 103/114 (90.35%), Query Frame = 0


Query: 2   AAAAALCFSLLFLSFLSEGDLLAVNG-EKTWCVARPSSDKATLLANINYACSQVDCRIMQ 61
           AAA A+ F LLFLS    GDLLAVNG +KTWCVARPSSD ATLLANINYACSQVDCRIMQ
Sbjct: 4   AAAVAISFLLLFLS----GDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQ 63

Query: 62  KGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114
           KGCPCFYPDNLIN AS+AMNLYY+S+GKNQWNCDFRGS LIVITDPSYGNC+YP
Sbjct: 64  KGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 113

[Close]

BLAST of MC11g_new0172 vs. NCBI nr
Match: XP_023525199.1 (major pollen allergen Ole e 10-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 197 bits (502), Expect = 3.29e-63
Identity = 94/114 (82.46%), Postives = 101/114 (88.60%), Query Frame = 0


Query: 1   MAAAAALCFSLLFLSFLSEGDLLAVNGEKTWCVARPSSDKATLLANINYACSQVDCRIMQ 60
           +A A  L F LLFLS    GDLLAVNG +TWCVARPSSD ATLLANI+YACSQVDCRIMQ
Sbjct: 5   VAGAVTLSFLLLFLS----GDLLAVNGYQTWCVARPSSDHATLLANIDYACSQVDCRIMQ 64

Query: 61  KGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114
           KGCPCFYPDNLIN AS+AMNLYY+S+GKNQWNCDFRGS LIVITDPSYGNC+YP
Sbjct: 65  KGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 114

[Close]

BLAST of MC11g_new0172 vs. ExPASy TrEMBL
Match: A0A6J1CBF4 (glucan endo-1,3-beta-D-glucosidase OS=Momordica charantia OX=3673 GN=LOC111009159 PE=4 SV=1)

HSP 1 Score: 241 bits (615), Expect = 9.21e-81
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MAAAAALCFSLLFLSFLSEGDLLAVNGEKTWCVARPSSDKATLLANINYACSQVDCRIMQ 60
           MAAAAALCFSLLFLSFLSEGDLLAVNGEKTWCVARPSSDKATLLANINYACSQVDCRIMQ
Sbjct: 1   MAAAAALCFSLLFLSFLSEGDLLAVNGEKTWCVARPSSDKATLLANINYACSQVDCRIMQ 60

Query: 61  KGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114
           KGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP
Sbjct: 61  KGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114

[Close]

BLAST of MC11g_new0172 vs. ExPASy TrEMBL
Match: A0A6J1FTX7 (major pollen allergen Ole e 10-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446803 PE=4 SV=1)

HSP 1 Score: 202 bits (514), Expect = 2.21e-65
Identity = 97/113 (85.84%), Postives = 102/113 (90.27%), Query Frame = 0


Query: 2   AAAAALCFSLLFLSFLSEGDLLAVNGEKTWCVARPSSDKATLLANINYACSQVDCRIMQK 61
           AAA AL F LLFLS    GDLLAVNG +TWCVARPSSD ATLLANINYACSQVDCRIMQK
Sbjct: 4   AAAVALSFLLLFLS----GDLLAVNGYQTWCVARPSSDHATLLANINYACSQVDCRIMQK 63

Query: 62  GCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114
           GCPCFYPDNLIN AS+AMNLYY+S+GKNQWNCDFRGS LIVITDPSYGNC+YP
Sbjct: 64  GCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 112

[Close]

BLAST of MC11g_new0172 vs. ExPASy TrEMBL
Match: A0A6J1FNL6 (glucan endo-1,3-beta-glucosidase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446803 PE=4 SV=1)

HSP 1 Score: 201 bits (510), Expect = 9.30e-65
Identity = 98/114 (85.96%), Postives = 103/114 (90.35%), Query Frame = 0


Query: 2   AAAAALCFSLLFLSFLSEGDLLAVNG-EKTWCVARPSSDKATLLANINYACSQVDCRIMQ 61
           AAA AL F LLFLS    GDLLAVNG +KTWCVARPSSD ATLLANINYACSQVDCRIMQ
Sbjct: 4   AAAVALSFLLLFLS----GDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQ 63

Query: 62  KGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114
           KGCPCFYPDNLIN AS+AMNLYY+S+GKNQWNCDFRGS LIVITDPSYGNC+YP
Sbjct: 64  KGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 113

[Close]

BLAST of MC11g_new0172 vs. ExPASy TrEMBL
Match: A0A5A7VF23 (Glucan endo-1,3-beta-D-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G004150 PE=4 SV=1)

HSP 1 Score: 197 bits (502), Expect = 1.65e-63
Identity = 88/111 (79.28%), Postives = 101/111 (90.99%), Query Frame = 0


Query: 4   AAALCFSLLFLSFLSEGDLLAVNGEKTWCVARPSSDKATLLANINYACSQVDCRIMQKGC 63
           AA+L F LLFLSFLS  DLLA+N +KTWC+ +PSSD+ATLLANINYAC+QVDCRIMQKGC
Sbjct: 5   AASLSFLLLFLSFLSGRDLLALNAQKTWCIPKPSSDQATLLANINYACAQVDCRIMQKGC 64

Query: 64  PCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114
           PC YPD LIN A++AM+LYYHS+GKNQWNCDFRGS L++ITDPSYGNCIYP
Sbjct: 65  PCSYPDTLINRAAIAMSLYYHSKGKNQWNCDFRGSGLVIITDPSYGNCIYP 115

[Close]

BLAST of MC11g_new0172 vs. ExPASy TrEMBL
Match: A0A1S3BUY7 (glucan endo-1,3-beta-D-glucosidase OS=Cucumis melo OX=3656 GN=LOC103493468 PE=4 SV=1)

HSP 1 Score: 197 bits (502), Expect = 1.65e-63
Identity = 88/111 (79.28%), Postives = 101/111 (90.99%), Query Frame = 0


Query: 4   AAALCFSLLFLSFLSEGDLLAVNGEKTWCVARPSSDKATLLANINYACSQVDCRIMQKGC 63
           AA+L F LLFLSFLS  DLLA+N +KTWC+ +PSSD+ATLLANINYAC+QVDCRIMQKGC
Sbjct: 5   AASLSFLLLFLSFLSGRDLLALNAQKTWCIPKPSSDQATLLANINYACAQVDCRIMQKGC 64

Query: 64  PCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114
           PC YPD LIN A++AM+LYYHS+GKNQWNCDFRGS L++ITDPSYGNCIYP
Sbjct: 65  PCSYPDTLINRAAIAMSLYYHSKGKNQWNCDFRGSGLVIITDPSYGNCIYP 115

[Close]

BLAST of MC11g_new0172 vs. TAIR 10
Match: AT1G78520.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 151.0 bits (380), Expect = 5.8e-37
Identity = 68/113 (60.18%), Postives = 85/113 (75.22%), Query Frame = 0


Query: 3   AAAALCFS-LLFLSFLSEGDLLAVNGE-KTWCVARPSSDKATLLANINYACSQVDCRIMQ 62
           A A +C S L+FL  +SE + + VN E KTWCVA+PSSD+  L  NIN+ACS VDCR++ 
Sbjct: 2   AKAWICLSFLIFLYLVSERNFINVNAETKTWCVAKPSSDQVALQDNINFACSHVDCRVLL 61

Query: 63  KGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIY 114
            GCPC+ P NLIN AS+AMNLYY + G+N WNC+F+ S LI IT+PSYGNC Y
Sbjct: 62  SGCPCYSPSNLINHASIAMNLYYQANGRNYWNCNFKNSGLITITNPSYGNCYY 114

[Close]

BLAST of MC11g_new0172 vs. TAIR 10
Match: AT2G43670.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 129.0 bits (323), Expect = 2.4e-30
Identity = 59/115 (51.30%), Postives = 81/115 (70.43%), Query Frame = 0


Query: 3   AAAALCFSLLFLSFLSEGDLLAV----NGEKTWCVARPSSDKATLLANINYACSQVDCRI 62
           A   LCF ++FL    EG+ + V         WCVA+PS+D   L  NIN+ACS++DC+I
Sbjct: 4   AQICLCF-IIFLYLWPEGNFIKVTKADRSAGDWCVAKPSTDNERLQENINFACSKIDCQI 63

Query: 63  MQKGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIY 114
           + +G  C+ PD++I+ ASVAMNLYY ++G++ WNC+F GS LI ITDPSYG+CIY
Sbjct: 64  ISEGGACYLPDSIISRASVAMNLYYQAQGRHFWNCNFEGSGLIGITDPSYGSCIY 117

[Close]

BLAST of MC11g_new0172 vs. TAIR 10
Match: AT2G43660.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 1.8e-22
Identity = 49/115 (42.61%), Postives = 68/115 (59.13%), Query Frame = 0


Query: 5   AALCFSLLFLSFLSEGDLLAVN----GEKTWCVARPSSDKATLLANINYAC--SQVDCRI 64
           A + F  + L  +S G  + VN    G+ +WCVA+P +    L+ N+N  C  S V C +
Sbjct: 4   AQIWFPFIILLCISSGSFMRVNAQAPGQGSWCVAKPGTPIKQLVKNLNNVCSNSSVHCEV 63

Query: 65  MQKGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIY 114
           + +G  C+ P NL N ASV MNLYY ++G+    CDF GS +I +TDPSY  CIY
Sbjct: 64  VSEGGACYDPINLYNSASVVMNLYYQNQGRQYSKCDFEGSGIISVTDPSYECCIY 118

[Close]

BLAST of MC11g_new0172 vs. TAIR 10
Match: AT3G28250.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 101.3 bits (251), Expect = 5.3e-22
Identity = 42/77 (54.55%), Postives = 54/77 (70.13%), Query Frame = 0


Query: 31  WCVARPSSDKATLLANINYACSQVDCRIMQKGCPCFYPDNLINGASVAMNLYYHSRGKNQ 90
           WCVA+P +    L+ N+NYACS VDC+I+     C+ PDN+ N ASV MNLYY + G+N 
Sbjct: 3   WCVAKPGTLTEQLINNLNYACSIVDCQIISTRGACYSPDNIYNMASVVMNLYYQAEGRNF 62

Query: 91  WNCDFRGSALIVITDPS 108
           WNC+F  S L+ ITDPS
Sbjct: 63  WNCNFGDSGLVAITDPS 79

[Close]

BLAST of MC11g_new0172 vs. TAIR 10
Match: AT2G43660.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 6.9e-22
Identity = 47/109 (43.12%), Postives = 65/109 (59.63%), Query Frame = 0


Query: 11  LLFLSFLSEGDLLAVN----GEKTWCVARPSSDKATLLANINYAC--SQVDCRIMQKGCP 70
           ++ L   S G  + VN    G+ +WCVA+P +    L+ N+N  C  S V C ++ +G  
Sbjct: 11  IILLCISSVGSFMRVNAQAPGQGSWCVAKPGTPIKQLVKNLNNVCSNSSVHCEVVSEGGA 70

Query: 71  CFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIY 114
           C+ P NL N ASV MNLYY ++G+    CDF GS +I +TDPSY  CIY
Sbjct: 71  CYDPINLYNSASVVMNLYYQNQGRQYSKCDFEGSGIISVTDPSYECCIY 119

The following BLAST results are available for this feature:

BLAST of MC11g_new0172 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94G86	3.1e-19	47.67	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]
P52409	1.2e-18	44.44	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]
Q84V39	1.9e-16	42.70	Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
Q8VYE5	4.2e-16	40.45	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
O65399	1.6e-15	40.91	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]

BLAST of MC11g_new0172 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137818.1	1.90e-80	100.00	glucan endo-1,3-beta-D-glucosidase [Momordica charantia]	[more]
XP_022941530.1	4.56e-65	85.84	major pollen allergen Ole e 10-like isoform X2 [Cucurbita moschata]	[more]
XP_022941529.1	1.92e-64	85.96	glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata] >KAG703746...	[more]
KAG6607951.1	3.88e-64	85.09	Glucan endo-1,3-beta-D-glucosidase, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_023525199.1	3.29e-63	82.46	major pollen allergen Ole e 10-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC11g_new0172 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CBF4	9.21e-81	100.00	glucan endo-1,3-beta-D-glucosidase OS=Momordica charantia OX=3673 GN=LOC11100915...	[more]
A0A6J1FTX7	2.21e-65	85.84	major pollen allergen Ole e 10-like isoform X2 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1FNL6	9.30e-65	85.96	glucan endo-1,3-beta-glucosidase-like isoform X1 OS=Cucurbita moschata OX=3662 G...	[more]
A0A5A7VF23	1.65e-63	79.28	Glucan endo-1,3-beta-D-glucosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BUY7	1.65e-63	79.28	glucan endo-1,3-beta-D-glucosidase OS=Cucumis melo OX=3656 GN=LOC103493468 PE=4 ...	[more]

BLAST of MC11g_new0172 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78520.1	5.8e-37	60.18	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G43670.1	2.4e-30	51.30	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G43660.1	1.8e-22	42.61	Carbohydrate-binding X8 domain superfamily protein	[more]
AT3G28250.1	5.3e-22	54.55	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G43660.2	6.9e-22	43.12	Carbohydrate-binding X8 domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 30..113 e-value: 3.9E-36 score: 136.0
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 30..99 e-value: 1.3E-18 score: 67.4
None	No IPR available	GENE3D	1.20.58.1040		coord: 25..111 e-value: 7.1E-10 score: 41.1
None	No IPR available	PANTHER	PTHR31044:SF38	MAJOR POLLEN ALLERGEN OLE E 10-LIKE	coord: 6..114
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 6..114

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC11g_new0172.1	MC11g_new0172.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0016787	hydrolase activity